Rh4BG376200

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001444
Physical Location & Seq
Forward (+)
53188856 .. 53191223
2368 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG376200.1

Sequence Viewer

Length: 1377 bp
ATGAAGACGAAGAAGAGGGTTCTGGCAGCAGTGGCTGCTTTGTGCTTGTTCTTCATCGTATTCCAGATCAGTGTCTCATCGACCTCCAGATTTCTTAATAATGCAGCATTTCCAGCTAAGACTCAAGAAACAACTCGTGTGGAATCAAAGCTGGTTAGTAAACAACTCCATCACCCACCGGAGTCTTTGGAACTTCGTTTGGAGAGTCCGATCATCTGTGACCGAACCTCTATACGTTATGATCTCTGCTCAATCAACGGCCCAACAGTTTTGGATCCGACCACATCGACTTTCTTCACTATGAATTCTACCGAACCACCATTGGTAGAGAGAATCAGACCCTATCCTCGAAAGTTCGAAGACCTCATAATGGCTGATATCAAGAACTTCACACTCACTGCCGGACCACGAATCCCAATGTGTAGAGTTCAACACAATGCTCCTGCGCTAGTATTCACCGCCGGTGGATACACCGGAAACTTTTGGCATGACTTCAACGATGGATTCATCCCTCTCTTTATCACCGCCAACACCATATTCCCAGATCAAGACTTCGTCATTGTGGTTTCCGAAGCTCCCAAGTGGTGGCCTAGTAAGTATGCCGATTTGCTACGCATGTTCACCAATCATCCTATTGTTAACCTAGGAAGTGATACCACCACCCATTGTTTTCCTTCTGCCAAGCTAGGCCTTATATCACATGGCTTCATGATCATAAACCAAACATTAATACCCAACTCAAAGACATACATGAATTTTCGTGGGCTCCTTGGCAAAGCCTATAACCAACTAGCCCAAATCCAAACCAAAATTTTCACCTCTAAGCCCACAAATCCTCGGCCCAGACTTGTTTTGGCGAGCCGACGTCATGCCACTGGACGCACGATCATGAACCAAGCCCAAGTTATTCGTTTGATCAAGAAAGTAGGTTTTGATGTGGTGGTGTTTGAGCCTAAAACCAAAACCTCATTGCTAGAATCTTATGCATTACTGAATTCAAGCCATGCTTTTGTTGGGGTTCATGGAGCTGCACTTACGCACTCATTGTTCCTCCGGCCGGGTGCGGTGTTCGTGCAGGTGGTGCCAATAGGAATAGGCTGGGCGGCGGAAGCGTTTTTCGGGAGAGTAGCAAAAGGGTTGAATTTGGAGTATATTGAGTACAAAATTGGTGTGGAAGAGAGTAGCTTGGTGGACAAGTATGGGAAGGACAGTTTGTTGGTTAGGGATCCATTTGCTCTTCAAAAAAGGGGCTGGCCCCCGGAGGTTATGAACACTTACTTGAAAGAACAGAATGTGAAACTGGATTTGGTTAGGTTCAAGAGATATTTGAAGAAAGCTTTTAAGAAAGCTAAGAGATTTATGGACAATGAGGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

458

Amino Acids

51.67

Weight (kDa)

9.78

Isoelectric Point (pI)

28.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 162 - 358 6e-15 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1066
AatII GACGTC 1 cut(s) 868
Acc36I ACCTGC 1 cut(s) 1066
AccB1I GGYRCC 1 cut(s) 1081
AccB7I CCANNNNNTGG 1 cut(s) 585
AciI CCGC 5 cut(s) 459, 525, 1064, 1103, 1106
AclWI GGATC 4 cut(s) 269, 282, 1220, 1233
AcoI YGGCCR 1 cut(s) 1055
AcsI RAATTY 5 cut(s) 304, 754, 810, 994, 1141
AcyI GRCGYC 1 cut(s) 865
AfaI GTAC 1 cut(s) 1160
AfiI CCNNNNNNNGG 3 cut(s) 370, 585, 1057
AgsI TTSAA 8 cut(s) 431, 496, 999, 1141, 1241, 1282, 1318, 1330
AjuI GAANNNNNNNTTGG 4 cut(s) 521, 553, 1289, 1321
AluBI AGCT 8 cut(s) 116, 151, 575, 685, 1028, 1185, 1337, 1349
AluI AGCT 8 cut(s) 116, 151, 575, 685, 1028, 1185, 1337, 1349
Alw26I GTCTC 1 cut(s) 79
AlwI GGATC 4 cut(s) 269, 282, 1220, 1233
AlwNI CAGNNNCTG 1 cut(s) 35
AoxI GGCC 6 cut(s) 259, 587, 688, 839, 1055, 1253
ApeKI GCWGC 4 cut(s) 26, 35, 104, 1028
ApoI RAATTY 5 cut(s) 304, 754, 810, 994, 1141
AseI ATTAAT 1 cut(s) 728
AspA2I CCTAGG 1 cut(s) 643
AspLEI GCGC 1 cut(s) 448
AspS9I GGNCC 4 cut(s) 260, 404, 840, 1254
AsuC2I CCSGG 2 cut(s) 1059, 1259
AsuHPI GGTGA 5 cut(s) 164, 448, 514, 613, 808
AsuII TTCGAA 1 cut(s) 357
AvaII GGWCC 1 cut(s) 404
AvrII CCTAGG 1 cut(s) 643
BamHI GGATCC 2 cut(s) 274, 1225
BanI GGYRCC 1 cut(s) 1081
BanII GRGCYC 1 cut(s) 768
BauI CACGAG 1 cut(s) 135
BbsI GAAGAC 2 cut(s) 11, 366
BbvI GCAGC 4 cut(s) 22, 38, 116, 1015
BccI CCATC 2 cut(s) 177, 494
BceAI ACGGC 1 cut(s) 274
BciVI GTATCC 1 cut(s) 461
BclI TGATCA 2 cut(s) 711, 915
BcnI CCSGG 2 cut(s) 1059, 1259
BcoDI GTCTC 1 cut(s) 79
BfaI CTAG 6 cut(s) 449, 591, 644, 686, 791, 974
BfuAI ACCTGC 1 cut(s) 1066
BfuI GTATCC 1 cut(s) 461
BisI GCNGC 5 cut(s) 27, 36, 105, 1029, 1104
BlnI CCTAGG 1 cut(s) 643
BlsI GCNGC 5 cut(s) 28, 37, 106, 1030, 1105
Bme1390I CCNGG 2 cut(s) 1059, 1259
Bme18I GGWCC 1 cut(s) 404
BmgT120I GGNCC 4 cut(s) 260, 404, 840, 1254
BmiI GGNNCC 5 cut(s) 276, 767, 1083, 1227, 1256
BmrFI CCNGG 2 cut(s) 1059, 1259
BpiI GAAGAC 2 cut(s) 11, 366
BpmI CTGGAG 1 cut(s) 70
Bpu14I TTCGAA 1 cut(s) 357
BpuEI CTTGAG 1 cut(s) 108
BpuMI CCSGG 2 cut(s) 1059, 1259
BsaHI GRCGYC 1 cut(s) 865
BsaJI CCNNGG 4 cut(s) 643, 769, 836, 1257
BsaWI WCCGGW 2 cut(s) 178, 473
Bsc4I CCNNNNNNNGG 3 cut(s) 370, 585, 1057
Bse118I RCCGGY 1 cut(s) 461
Bse1I ACTGG 2 cut(s) 880, 1305
Bse3DI GCAATG 1 cut(s) 968
BseDI CCNNGG 4 cut(s) 643, 769, 836, 1257
BseGI GGATG 2 cut(s) 507, 628
BseLI CCNNNNNNNGG 3 cut(s) 370, 585, 1057
BseMI GCAATG 1 cut(s) 968
BseNI ACTGG 2 cut(s) 880, 1305
BseX3I CGGCCG 1 cut(s) 1055
BseXI GCAGC 4 cut(s) 22, 38, 116, 1015
BseYI CCCAGC 1 cut(s) 1098
BsgI GTGCAG 2 cut(s) 1014, 1094
Bsh1285I CGRYCG 1 cut(s) 1058
BshFI GGCC 6 cut(s) 261, 589, 690, 841, 1057, 1255
BshNI GGYRCC 1 cut(s) 1081
BsiEI CGRYCG 1 cut(s) 1058
BsiSI CCGG 7 cut(s) 179, 402, 462, 474, 1054, 1058, 1259
BslI CCNNNNNNNGG 3 cut(s) 370, 585, 1057
BsmAI GTCTC 1 cut(s) 79
BsnI GGCC 6 cut(s) 261, 589, 690, 841, 1057, 1255
Bsp119I TTCGAA 1 cut(s) 357
Bsp1286I GDGCHC 1 cut(s) 768
Bsp143I GATC 9 cut(s) 66, 210, 241, 274, 544, 711, 885, 915, 1225
BspACI CCGC 5 cut(s) 459, 525, 1064, 1103, 1106
BspANI GGCC 6 cut(s) 261, 589, 690, 841, 1057, 1255
BspHI TCATGA 2 cut(s) 708, 888
BspLI GGNNCC 5 cut(s) 276, 767, 1083, 1227, 1256
BspMI ACCTGC 1 cut(s) 1066
BspPI GGATC 4 cut(s) 269, 282, 1220, 1233
BspQI GCTCTTC 1 cut(s) 1242
BspT104I TTCGAA 1 cut(s) 357
BspT107I GGYRCC 1 cut(s) 1081
BsrDI GCAATG 1 cut(s) 968
BsrFI RCCGGY 1 cut(s) 461
BsrI ACTGG 2 cut(s) 880, 1305
BssAI RCCGGY 1 cut(s) 461
BssECI CCNNGG 4 cut(s) 643, 769, 836, 1257
BssMI GATC 9 cut(s) 66, 210, 241, 274, 544, 711, 885, 915, 1225
BssNI GRCGYC 1 cut(s) 865
BssSI CACGAG 1 cut(s) 135
BssT1I CCWWGG 2 cut(s) 643, 769
Bst2BI CACGAG 1 cut(s) 135
Bst4CI ACNGT 2 cut(s) 268, 1211
Bst6I CTCTTC 3 cut(s) 8, 1170, 1242
BstACI GRCGYC 1 cut(s) 865
BstAPI GCANNNNNTGC 2 cut(s) 35, 1081
BstBI TTCGAA 1 cut(s) 357
BstC8I GCNNGC 2 cut(s) 859, 1253
BstDEI CTNAG 3 cut(s) 117, 822, 1350
BstF5I GGATG 2 cut(s) 507, 628
BstHHI GCGC 1 cut(s) 448
BstKTI GATC 9 cut(s) 69, 213, 244, 277, 547, 714, 888, 918, 1228
BstMAI GTCTC 1 cut(s) 79
BstMBI GATC 9 cut(s) 66, 210, 241, 274, 544, 711, 885, 915, 1225
BstMCI CGRYCG 1 cut(s) 1058
BstMWI GCNNNNNNNGC 5 cut(s) 32, 35, 113, 1081, 1109
BstNSI RCATGY 1 cut(s) 619
BstSCI CCNGG 2 cut(s) 1057, 1257
BstV1I GCAGC 4 cut(s) 22, 38, 116, 1015
BstV2I GAAGAC 2 cut(s) 11, 366
BstX2I RGATCY 2 cut(s) 274, 1225
BstYI RGATCY 2 cut(s) 274, 1225
BstZI CGGCCG 1 cut(s) 1055
BsuI GTATCC 1 cut(s) 461
BsuRI GGCC 6 cut(s) 261, 589, 690, 841, 1057, 1255
BtsCI GGATG 2 cut(s) 507, 628
BtsI GCAGTG 2 cut(s) 36, 396
BtsIMutI CAGTG 4 cut(s) 36, 76, 396, 873
BveI ACCTGC 1 cut(s) 1066
Cac8I GCNNGC 2 cut(s) 859, 1253
CaiI CAGNNNCTG 1 cut(s) 35
CciI TCATGA 2 cut(s) 708, 888
CfoI GCGC 1 cut(s) 448
Cfr10I RCCGGY 1 cut(s) 461
Cfr13I GGNCC 4 cut(s) 260, 404, 840, 1254
CseI GACGC 1 cut(s) 888
Csp6I GTAC 1 cut(s) 1159
CspCI CAANNNNNGTGG 2 cut(s) 120, 155
CviAII CATG 9 cut(s) 488, 616, 701, 709, 751, 869, 889, 1004, 1022
CviQI GTAC 1 cut(s) 1159
DdeI CTNAG 3 cut(s) 117, 822, 1350
DpnI GATC 9 cut(s) 68, 212, 243, 276, 546, 713, 887, 917, 1227
DpnII GATC 9 cut(s) 66, 210, 241, 274, 544, 711, 885, 915, 1225
EaeI YGGCCR 1 cut(s) 1055
EagI CGGCCG 1 cut(s) 1055
Eam1104I CTCTTC 3 cut(s) 8, 1170, 1242
EarI CTCTTC 3 cut(s) 8, 1170, 1242
EciI GGCGGA 1 cut(s) 1121
EclXI CGGCCG 1 cut(s) 1055
Eco130I CCWWGG 2 cut(s) 643, 769
Eco147I AGGCCT 1 cut(s) 690
Eco24I GRGCYC 1 cut(s) 768
Eco32I GATATC 1 cut(s) 379
Eco47I GGWCC 1 cut(s) 404
Eco52I CGGCCG 1 cut(s) 1055
EcoRI GAATTC 2 cut(s) 304, 994
EcoRV GATATC 1 cut(s) 379
EcoT14I CCWWGG 2 cut(s) 643, 769
EcoT22I ATGCAT 1 cut(s) 988
EcoT38I GRGCYC 1 cut(s) 768
ErhI CCWWGG 2 cut(s) 643, 769
FaeI CATG 9 cut(s) 491, 619, 704, 712, 754, 872, 892, 1007, 1025
FalI AAGNNNNNCTT 2 cut(s) 991, 1023
FatI CATG 9 cut(s) 487, 615, 700, 708, 750, 868, 888, 1003, 1021
FbaI TGATCA 2 cut(s) 711, 915
Fnu4HI GCNGC 5 cut(s) 27, 36, 105, 1029, 1104
FokI GGATG 2 cut(s) 494, 615
FriOI GRGCYC 1 cut(s) 768
Fsp4HI GCNGC 5 cut(s) 27, 36, 105, 1029, 1104
FspBI CTAG 6 cut(s) 449, 591, 644, 686, 791, 974
GlaI GCGC 1 cut(s) 447
GluI GCNGC 5 cut(s) 27, 36, 105, 1029, 1104
GsaI CCCAGC 1 cut(s) 1102
GsuI CTGGAG 1 cut(s) 70
HaeIII GGCC 6 cut(s) 261, 589, 690, 841, 1057, 1255
HapII CCGG 7 cut(s) 179, 402, 462, 474, 1054, 1058, 1259
HgaI GACGC 1 cut(s) 888
HhaI GCGC 1 cut(s) 448
Hin1I GRCGYC 1 cut(s) 865
Hin1II CATG 9 cut(s) 491, 619, 704, 712, 754, 872, 892, 1007, 1025
Hin6I GCGC 1 cut(s) 446
HinP1I GCGC 1 cut(s) 446
HincII GTYRAC 1 cut(s) 640
HindII GTYRAC 1 cut(s) 640
HindIII AAGCTT 1 cut(s) 1335
HinfI GANTC 8 cut(s) 121, 143, 182, 205, 333, 411, 504, 977
HpaI GTTAAC 1 cut(s) 640
HpaII CCGG 7 cut(s) 179, 402, 462, 474, 1054, 1058, 1259
HphI GGTGA 5 cut(s) 164, 448, 514, 613, 808
Hpy166II GTNNAC 4 cut(s) 161, 621, 640, 1192
Hpy188I TCNGA 4 cut(s) 210, 279, 338, 571
Hpy8I GTNNAC 4 cut(s) 161, 621, 640, 1192
Hpy99I CGWCG 1 cut(s) 867
HpyAV CCTTC 2 cut(s) 684, 1198
HpyCH4III ACNGT 2 cut(s) 268, 1211
HpyCH4IV ACGT 2 cut(s) 235, 865
HpyCH4V TGCA 4 cut(s) 104, 986, 1031, 1075
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 35, 113, 1081, 1109
HpyF3I CTNAG 3 cut(s) 117, 822, 1350
HpySE526I ACGT 2 cut(s) 235, 865
Hsp92I GRCGYC 1 cut(s) 865
Hsp92II CATG 9 cut(s) 491, 619, 704, 712, 754, 872, 892, 1007, 1025
HspAI GCGC 1 cut(s) 446
Ksp22I TGATCA 2 cut(s) 711, 915
KspAI GTTAAC 1 cut(s) 640
Kzo9I GATC 9 cut(s) 66, 210, 241, 274, 544, 711, 885, 915, 1225
LguI GCTCTTC 1 cut(s) 1242
LmnI GCTCC 4 cut(s) 445, 580, 771, 1025
Lsp1109I GCAGC 4 cut(s) 22, 38, 116, 1015
MaeI CTAG 6 cut(s) 449, 591, 644, 686, 791, 974
MaeII ACGT 2 cut(s) 235, 865
MaeIII GTNAC 1 cut(s) 218
MalI GATC 9 cut(s) 68, 212, 243, 276, 546, 713, 887, 917, 1227
MboI GATC 9 cut(s) 66, 210, 241, 274, 544, 711, 885, 915, 1225
MboII GAAGA 9 cut(s) 16, 22, 25, 43, 286, 371, 1187, 1229, 1342
MflI RGATCY 2 cut(s) 274, 1225
MhlI GDGCHC 1 cut(s) 768
MluCI AATT 6 cut(s) 304, 754, 810, 994, 1141, 1164
MlyI GAGTC 3 cut(s) 115, 191, 214
MmeI TCCRAC 1 cut(s) 302
Mph1103I ATGCAT 1 cut(s) 988
MseI TTAA 4 cut(s) 96, 639, 728, 1341
MslI CAYNNNNRTG 1 cut(s) 887
MspI CCGG 7 cut(s) 179, 402, 462, 474, 1054, 1058, 1259
MspR9I CCNGG 2 cut(s) 1059, 1259
MwoI GCNNNNNNNGC 5 cut(s) 32, 35, 113, 1081, 1109
NciI CCSGG 2 cut(s) 1059, 1259
NdeII GATC 9 cut(s) 66, 210, 241, 274, 544, 711, 885, 915, 1225
NlaIII CATG 9 cut(s) 491, 619, 704, 712, 754, 872, 892, 1007, 1025
NlaIV GGNNCC 5 cut(s) 276, 767, 1083, 1227, 1256
NmeAIII GCCGAG 1 cut(s) 817
NmuCI GTSAC 1 cut(s) 218
NsiI ATGCAT 1 cut(s) 988
NspI RCATGY 1 cut(s) 619
NspV TTCGAA 1 cut(s) 357
PagI TCATGA 2 cut(s) 708, 888
PaqCI CACCTGC 1 cut(s) 1066
PceI AGGCCT 1 cut(s) 690
PciSI GCTCTTC 1 cut(s) 1242
PfeI GAWTC 5 cut(s) 143, 333, 411, 504, 977
PflFI GACNNNGTC 1 cut(s) 554
PflMI CCANNNNNTGG 1 cut(s) 585
PkrI GCNGC 5 cut(s) 28, 37, 106, 1030, 1105
PleI GAGTC 3 cut(s) 115, 190, 213
PpsI GAGTC 3 cut(s) 115, 190, 213
PshBI ATTAAT 1 cut(s) 728
PspFI CCCAGC 1 cut(s) 1098
PspN4I GGNNCC 5 cut(s) 276, 767, 1083, 1227, 1256
PspPI GGNCC 4 cut(s) 260, 404, 840, 1254
PstNI CAGNNNCTG 1 cut(s) 35
PsuI RGATCY 2 cut(s) 274, 1225
PsyI GACNNNGTC 1 cut(s) 554
RsaI GTAC 1 cut(s) 1160
RsaNI GTAC 1 cut(s) 1159
RseI CAYNNNNRTG 1 cut(s) 887
SapI GCTCTTC 1 cut(s) 1242
SaqAI TTAA 4 cut(s) 96, 639, 728, 1341
SatI GCNGC 5 cut(s) 27, 36, 105, 1029, 1104
Sau3AI GATC 9 cut(s) 66, 210, 241, 274, 544, 711, 885, 915, 1225
Sau96I GGNCC 4 cut(s) 260, 404, 840, 1254
SchI GAGTC 3 cut(s) 115, 191, 214
ScrFI CCNGG 2 cut(s) 1059, 1259
SduI GDGCHC 1 cut(s) 768
SfuI TTCGAA 1 cut(s) 357
SgrAI CRCCGGYG 1 cut(s) 461
SinI GGWCC 1 cut(s) 404
SmiMI CAYNNNNRTG 1 cut(s) 887
SmlI CTYRAG 1 cut(s) 123
SmoI CTYRAG 1 cut(s) 123
Sse9I AATT 6 cut(s) 304, 754, 810, 994, 1141, 1164
SseBI AGGCCT 1 cut(s) 690
SsiI CCGC 5 cut(s) 459, 525, 1064, 1103, 1106
SspMI CTAG 6 cut(s) 449, 591, 644, 686, 791, 974
StuI AGGCCT 1 cut(s) 690
StyD4I CCNGG 2 cut(s) 1057, 1257
StyI CCWWGG 2 cut(s) 643, 769
TaaI ACNGT 2 cut(s) 268, 1211
TaiI ACGT 2 cut(s) 238, 868
TaqI TCGA 4 cut(s) 80, 287, 349, 357
TaqII GACCGA 1 cut(s) 237
TasI AATT 6 cut(s) 304, 754, 810, 994, 1141, 1164
TatI WGTACW 1 cut(s) 1158
TauI GCSGC 1 cut(s) 1106
TfiI GAWTC 5 cut(s) 143, 333, 411, 504, 977
Tru1I TTAA 4 cut(s) 96, 639, 728, 1341
Tru9I TTAA 4 cut(s) 96, 639, 728, 1341
TscAI CASTG 4 cut(s) 36, 76, 403, 880
TseFI GTSAC 1 cut(s) 218
TseI GCWGC 4 cut(s) 26, 35, 104, 1028
Tsp45I GTSAC 1 cut(s) 218
TspDTI ATGAA 9 cut(s) 17, 43, 317, 496, 697, 767, 905, 1010, 1283
TspRI CASTG 4 cut(s) 36, 76, 403, 880
Tth111I GACNNNGTC 1 cut(s) 554
Van91I CCANNNNNTGG 1 cut(s) 585
VpaK11BI GGWCC 1 cut(s) 404
VspI ATTAAT 1 cut(s) 728
XapI RAATTY 5 cut(s) 304, 754, 810, 994, 1141
XceI RCATGY 1 cut(s) 619
XcmI CCANNNNNNNNNTGG 2 cut(s) 850, 1010
XmaJI CCTAGG 1 cut(s) 643
XspI CTAG 6 cut(s) 449, 591, 644, 686, 791, 974
ZraI GACGTC 1 cut(s) 866
Zsp2I ATGCAT 1 cut(s) 988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.