pycom16g05720

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001444
Physical Location & Seq
Forward (+)
3670752 .. 3672452
1701 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g05720.1

Sequence Viewer

Length: 1398 bp
ATGAAGAAGACAATTTCCTGCAGGGCTGCGACGGTGGCCTTGGCGGCCTTGTGTTTGTTTTTCATCGTGTTCGAAATCAGCGTCTCTTCAACCTCCAGATTTCTTAAATATGCTGCTACTACTACTGCTACATATTCAGCCAAGACTCAAGAGGAAGGGAATCATGAAAAAACGAAACTGATCAATCGGCCAAACTCTAACCCTTCAGAATACTTGCAACTTCCTGTGAAAAGACGAATCATCTGCGACCGTACAGATGTACGCTATGATCTTTGTTCAATCAACGGTCCAACTGTCTTGGACCCAAACACCTTCACATTCTTCACTATGGGCTCAGCCCAACCCCCACAGGTGGAAAAAGTCCAGCCCTACCCTCGAAAGTTCGAAGGCTTCATAATGCCTCACATCAAGAACCTCACCCTCACTTCCGGCCCACAAAGCCCACCATGTAAAGTCCGGCACAGTGTTCCGGCCCTAGTATTCAGTGCGGGAGGGTACACCGGAAACTTCTTCCATGATTTCAATGACGGGTTCATTCCTCTCTTCATCACCGTCAACACAATCTTCCCTGATCAAGAGATCGTGATCGTGGTTTCTGAAGCTCCCAATTGGTGGCCTAGTAAGTATGCAGATCTACTAGCAATGTTCACCAAGTACCCCATCATTATACTAAAAAATGCTACTGCCACCCATTGTTTTCCCTCAGCCACCATAGGCCTCATTTCACATGGTTTCATGACCATAAACGAAACATTACTACCAAATCCAAAATCGTTCATGGATTTTCGGGTCCTCCTAGAAAAAGCCTATGGCCCAAAAGCCCAGCCCAAAGTCTTGACATCCAAGCCCACAAGGTCACGCCCGAGACTCGTGTTGGCCAACCGCGAAATGGCACACGCGCGCGTGATTATGAACCAAAAACAATTGATTAGGTTAATTAAAAAAGTGGGTTTTGAAGTGATTATGTTTAATCCCAAGAGGATGACCCCGTTGCATGAATCTTATGCGTTATTGAATTCAAGCCATGCTATGATTGGGGTCCATGGAGCTGCATTGACGCACTCTTTTTTTCTTCGACCCGGTTCTGTGTTTGTTCAAGTGGTTCCGATTGGGATTGAATGGGGTGCATATGCTTTTTTTGGGAGGATGGCTAGGGCTTTGGATTTGGGATATTCGGAGTATAAGATTGGTGTGAACGAGAGTAGCTTGGTCGACAAGTATGGGAAGGAAAATTTGTTGCTAAAGGACCCATTTGCCCTTCAGAAAACGGGTTGGCCACCTGAGGTTATGAACATTTACCTCAAGGAGCAAAACGTCAAACTGGATTTGGAAAGATTTAACAGTTGTTTGAAGAAGGCTTATACAAAGGCTACCAGATTTATGGAGAAAAATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

466

Amino Acids

52.38

Weight (kDa)

9.6

Isoelectric Point (pI)

35.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 258 - 365 2e-13 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 612
AccI GTMKAC 1 cut(s) 1212
AccII CGCG 4 cut(s) 885, 899, 901, 903
AciI CCGC 3 cut(s) 44, 488, 883
AcoI YGGCCR 3 cut(s) 188, 876, 1274
AcsI RAATTY 2 cut(s) 1015, 1231
AcuI CTGAAG 3 cut(s) 189, 618, 1244
AfaI GTAC 4 cut(s) 253, 261, 497, 656
AfiI CCNNNNNNNGG 3 cut(s) 352, 612, 889
AgsI TTSAA 9 cut(s) 90, 279, 523, 956, 1015, 1020, 1097, 1118, 1351
AluBI AGCT 3 cut(s) 602, 1049, 1206
AluI AGCT 3 cut(s) 602, 1049, 1206
Alw26I GTCTC 2 cut(s) 88, 859
Ama87I CYCGRG 1 cut(s) 862
ApeKI GCWGC 3 cut(s) 26, 113, 1049
ApoI RAATTY 2 cut(s) 1015, 1231
ArsI GACNNNNNNTTYG 2 cut(s) 66, 98
AspLEI GCGC 2 cut(s) 901, 903
AspS9I GGNCC 8 cut(s) 287, 301, 431, 472, 790, 812, 1039, 1246
AsuC2I CCSGG 1 cut(s) 1080
AsuHPI GGTGA 3 cut(s) 409, 541, 640
AsuII TTCGAA 2 cut(s) 72, 384
AvaI CYCGRG 1 cut(s) 862
AvaII GGWCC 5 cut(s) 287, 301, 790, 1039, 1246
AxyI CCTNAGG 1 cut(s) 1281
BalI TGGCCA 2 cut(s) 878, 1276
BanII GRGCYC 1 cut(s) 335
BauI CACGAG 1 cut(s) 869
BbsI GAAGAC 1 cut(s) 14
BbvCI CCTCAGC 1 cut(s) 703
BbvI GCAGC 3 cut(s) 13, 100, 1036
BccI CCATC 2 cut(s) 668, 1141
BcgI CGANNNNNNTGC 2 cut(s) 225, 259
BclI TGATCA 2 cut(s) 180, 571
BcnI CCSGG 1 cut(s) 1080
BcoDI GTCTC 2 cut(s) 88, 859
BfaI CTAG 5 cut(s) 476, 618, 638, 797, 1152
BfmI CTRYAG 1 cut(s) 19
BglI GCCNNNNNGGC 1 cut(s) 44
BglII AGATCT 1 cut(s) 631
BisI GCNGC 4 cut(s) 27, 45, 114, 1050
BlpI GCTNAGC 1 cut(s) 334
BlsI GCNGC 4 cut(s) 28, 46, 115, 1051
Bme1390I CCNGG 1 cut(s) 1080
Bme18I GGWCC 5 cut(s) 287, 301, 790, 1039, 1246
BmeT110I CYCGRG 1 cut(s) 862
BmgT120I GGNCC 8 cut(s) 287, 301, 431, 472, 790, 812, 1039, 1246
BmiI GGNNCC 5 cut(s) 303, 791, 1040, 1104, 1248
BmrFI CCNGG 1 cut(s) 1080
BpiI GAAGAC 1 cut(s) 14
BpmI CTGGAG 1 cut(s) 79
Bpu10I CCTNAGC 1 cut(s) 703
Bpu1102I GCTNAGC 1 cut(s) 334
Bpu14I TTCGAA 2 cut(s) 72, 384
BpuEI CTTGAG 2 cut(s) 132, 1286
BpuMI CCSGG 1 cut(s) 1080
BsaBI GATNNNNATC 1 cut(s) 584
BsaJI CCNNGG 2 cut(s) 39, 1042
BsaWI WCCGGW 1 cut(s) 500
Bsc4I CCNNNNNNNGG 3 cut(s) 352, 612, 889
Bse1I ACTGG 1 cut(s) 1326
Bse21I CCTNAGG 1 cut(s) 1281
Bse3DI GCAATG 1 cut(s) 648
Bse8I GATNNNNATC 1 cut(s) 584
BseDI CCNNGG 2 cut(s) 39, 1042
BseGI GGATG 3 cut(s) 839, 987, 1152
BseJI GATNNNNATC 1 cut(s) 584
BseLI CCNNNNNNNGG 3 cut(s) 352, 612, 889
BseMI GCAATG 1 cut(s) 648
BseMII CTCAG 3 cut(s) 348, 717, 1272
BseNI ACTGG 1 cut(s) 1326
BsePI GCGCGC 1 cut(s) 899
BseXI GCAGC 3 cut(s) 13, 100, 1036
BseYI CCCAGC 1 cut(s) 822
Bsh1236I CGCG 4 cut(s) 885, 899, 901, 903
Bsh1285I CGRYCG 1 cut(s) 250
BsiEI CGRYCG 1 cut(s) 250
BsiHKCI CYCGRG 1 cut(s) 862
BsiSI CCGG 5 cut(s) 429, 457, 470, 501, 1080
BslI CCNNNNNNNGG 3 cut(s) 352, 612, 889
BsmAI GTCTC 2 cut(s) 88, 859
BsmBI CGTCTC 1 cut(s) 88
BsoBI CYCGRG 1 cut(s) 862
Bsp119I TTCGAA 2 cut(s) 72, 384
Bsp1286I GDGCHC 1 cut(s) 335
Bsp143I GATC 6 cut(s) 180, 268, 571, 579, 585, 631
Bsp1720I GCTNAGC 1 cut(s) 334
Bsp19I CCATGG 1 cut(s) 1042
BspACI CCGC 3 cut(s) 44, 488, 883
BspCNI CTCAG 3 cut(s) 347, 716, 1273
BspFNI CGCG 4 cut(s) 885, 899, 901, 903
BspHI TCATGA 2 cut(s) 163, 735
BspLI GGNNCC 5 cut(s) 303, 791, 1040, 1104, 1248
BspMAI CTGCAG 1 cut(s) 23
BspT104I TTCGAA 2 cut(s) 72, 384
BsrDI GCAATG 1 cut(s) 648
BsrI ACTGG 1 cut(s) 1326
BssECI CCNNGG 2 cut(s) 39, 1042
BssHII GCGCGC 1 cut(s) 899
BssMI GATC 6 cut(s) 180, 268, 571, 579, 585, 631
BssSI CACGAG 1 cut(s) 869
BssT1I CCWWGG 2 cut(s) 39, 1042
Bst2BI CACGAG 1 cut(s) 869
Bst4CI ACNGT 7 cut(s) 34, 251, 287, 295, 464, 553, 1343
Bst6I CTCTTC 2 cut(s) 91, 548
BstBI TTCGAA 2 cut(s) 72, 384
BstC8I GCNNGC 1 cut(s) 901
BstDEI CTNAG 3 cut(s) 334, 703, 1281
BstDSI CCRYGG 1 cut(s) 1042
BstF5I GGATG 3 cut(s) 839, 987, 1152
BstFNI CGCG 4 cut(s) 885, 899, 901, 903
BstHHI GCGC 2 cut(s) 901, 903
BstKTI GATC 6 cut(s) 183, 271, 574, 582, 588, 634
BstMAI GTCTC 2 cut(s) 88, 859
BstMBI GATC 6 cut(s) 180, 268, 571, 579, 585, 631
BstMCI CGRYCG 1 cut(s) 250
BstMWI GCNNNNNNNGC 3 cut(s) 35, 44, 438
BstSCI CCNGG 1 cut(s) 1078
BstSFI CTRYAG 1 cut(s) 19
BstUI CGCG 4 cut(s) 885, 899, 901, 903
BstV1I GCAGC 3 cut(s) 13, 100, 1036
BstV2I GAAGAC 1 cut(s) 14
BstX2I RGATCY 1 cut(s) 631
BstXI CCANNNNNNTGG 1 cut(s) 1381
BstYI RGATCY 1 cut(s) 631
Bsu36I CCTNAGG 1 cut(s) 1281
BtgI CCRYGG 1 cut(s) 1042
BtsCI GGATG 3 cut(s) 839, 987, 1152
BtsIMutI CAGTG 2 cut(s) 469, 490
Cac8I GCNNGC 1 cut(s) 901
CciI TCATGA 2 cut(s) 163, 735
CfoI GCGC 2 cut(s) 901, 903
Cfr13I GGNCC 8 cut(s) 287, 301, 431, 472, 790, 812, 1039, 1246
CseI GACGC 2 cut(s) 70, 1066
Csp6I GTAC 4 cut(s) 252, 260, 496, 655
CviAII CATG 9 cut(s) 164, 447, 515, 728, 736, 778, 995, 1025, 1043
CviQI GTAC 4 cut(s) 252, 260, 496, 655
DdeI CTNAG 3 cut(s) 334, 703, 1281
DpnI GATC 6 cut(s) 182, 270, 573, 581, 587, 633
DpnII GATC 6 cut(s) 180, 268, 571, 579, 585, 631
EaeI YGGCCR 3 cut(s) 188, 876, 1274
Eam1104I CTCTTC 2 cut(s) 91, 548
EarI CTCTTC 2 cut(s) 91, 548
Eco130I CCWWGG 2 cut(s) 39, 1042
Eco147I AGGCCT 1 cut(s) 717
Eco24I GRGCYC 1 cut(s) 335
Eco47I GGWCC 5 cut(s) 287, 301, 790, 1039, 1246
Eco57I CTGAAG 3 cut(s) 189, 618, 1244
Eco81I CCTNAGG 1 cut(s) 1281
Eco88I CYCGRG 1 cut(s) 862
EcoO109I RGGNCCY 2 cut(s) 790, 1246
EcoRI GAATTC 1 cut(s) 1015
EcoT14I CCWWGG 2 cut(s) 39, 1042
EcoT38I GRGCYC 1 cut(s) 335
ErhI CCWWGG 2 cut(s) 39, 1042
Esp3I CGTCTC 1 cut(s) 88
FaeI CATG 9 cut(s) 167, 450, 518, 731, 739, 781, 998, 1028, 1046
FatI CATG 9 cut(s) 163, 446, 514, 727, 735, 777, 994, 1024, 1042
FauI CCCGC 1 cut(s) 481
FauNDI CATATG 1 cut(s) 1129
FbaI TGATCA 2 cut(s) 180, 571
FblI GTMKAC 1 cut(s) 1212
Fnu4HI GCNGC 4 cut(s) 27, 45, 114, 1050
FokI GGATG 3 cut(s) 826, 994, 1159
FriOI GRGCYC 1 cut(s) 335
Fsp4HI GCNGC 4 cut(s) 27, 45, 114, 1050
FspBI CTAG 5 cut(s) 476, 618, 638, 797, 1152
GlaI GCGC 2 cut(s) 900, 902
GluI GCNGC 4 cut(s) 27, 45, 114, 1050
GsaI CCCAGC 1 cut(s) 826
GsuI CTGGAG 1 cut(s) 79
HapII CCGG 5 cut(s) 429, 457, 470, 501, 1080
HgaI GACGC 2 cut(s) 70, 1066
HhaI GCGC 2 cut(s) 901, 903
Hin1II CATG 9 cut(s) 167, 450, 518, 731, 739, 781, 998, 1028, 1046
Hin6I GCGC 2 cut(s) 899, 901
HinP1I GCGC 2 cut(s) 899, 901
HincII GTYRAC 2 cut(s) 556, 1213
HindII GTYRAC 2 cut(s) 556, 1213
HinfI GANTC 5 cut(s) 145, 160, 237, 867, 998
HpaII CCGG 5 cut(s) 429, 457, 470, 501, 1080
HphI GGTGA 3 cut(s) 409, 541, 640
Hpy166II GTNNAC 5 cut(s) 498, 556, 648, 1195, 1213
Hpy188I TCNGA 5 cut(s) 208, 598, 1107, 1177, 1263
Hpy188III TCNNGA 8 cut(s) 96, 149, 164, 409, 575, 583, 736, 835
Hpy8I GTNNAC 5 cut(s) 498, 556, 648, 1195, 1213
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 7 cut(s) 149, 213, 322, 380, 1219, 1268, 1348
HpyCH4III ACNGT 7 cut(s) 34, 251, 287, 295, 464, 553, 1343
HpyCH4IV ACGT 1 cut(s) 1314
HpyCH4V TGCA 6 cut(s) 21, 217, 629, 994, 1052, 1127
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 44, 438
HpyF3I CTNAG 3 cut(s) 334, 703, 1281
HpySE526I ACGT 1 cut(s) 1314
Hsp92II CATG 9 cut(s) 167, 450, 518, 731, 739, 781, 998, 1028, 1046
HspAI GCGC 2 cut(s) 899, 901
Ksp22I TGATCA 2 cut(s) 180, 571
Kzo9I GATC 6 cut(s) 180, 268, 571, 579, 585, 631
LmnI GCTCC 3 cut(s) 607, 1046, 1306
Lsp1109I GCAGC 3 cut(s) 13, 100, 1036
MaeI CTAG 5 cut(s) 476, 618, 638, 797, 1152
MaeII ACGT 1 cut(s) 1314
MaeIII GTNAC 1 cut(s) 855
MalI GATC 6 cut(s) 182, 270, 573, 581, 587, 633
MauBI CGCGCGCG 1 cut(s) 899
MboI GATC 6 cut(s) 180, 268, 571, 579, 585, 631
MboII GAAGA 9 cut(s) 16, 19, 78, 313, 502, 535, 556, 1064, 1363
MfeI CAATTG 2 cut(s) 607, 923
MflI RGATCY 1 cut(s) 631
MhlI GDGCHC 1 cut(s) 335
MlsI TGGCCA 2 cut(s) 878, 1276
MluCI AATT 6 cut(s) 12, 607, 923, 936, 1015, 1231
MluNI TGGCCA 2 cut(s) 878, 1276
MlyI GAGTC 2 cut(s) 139, 861
MmeI TCCRAC 1 cut(s) 314
Mox20I TGGCCA 2 cut(s) 878, 1276
MscI TGGCCA 2 cut(s) 878, 1276
MseI TTAA 6 cut(s) 105, 935, 939, 969, 1338, 1396
Msp20I TGGCCA 2 cut(s) 878, 1276
MspI CCGG 5 cut(s) 429, 457, 470, 501, 1080
MspR9I CCNGG 1 cut(s) 1080
MunI CAATTG 2 cut(s) 607, 923
MvnI CGCG 4 cut(s) 885, 899, 901, 903
MwoI GCNNNNNNNGC 3 cut(s) 35, 44, 438
NciI CCSGG 1 cut(s) 1080
NcoI CCATGG 1 cut(s) 1042
NdeI CATATG 1 cut(s) 1129
NdeII GATC 6 cut(s) 180, 268, 571, 579, 585, 631
NlaIII CATG 9 cut(s) 167, 450, 518, 731, 739, 781, 998, 1028, 1046
NlaIV GGNNCC 5 cut(s) 303, 791, 1040, 1104, 1248
NmuCI GTSAC 1 cut(s) 855
NspV TTCGAA 2 cut(s) 72, 384
PacI TTAATTAA 1 cut(s) 939
PagI TCATGA 2 cut(s) 163, 735
PauI GCGCGC 1 cut(s) 899
PceI AGGCCT 1 cut(s) 717
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PfeI GAWTC 3 cut(s) 160, 237, 998
PflMI CCANNNNNTGG 1 cut(s) 612
PkrI GCNGC 4 cut(s) 28, 46, 115, 1051
PleI GAGTC 2 cut(s) 139, 861
PpsI GAGTC 2 cut(s) 139, 861
PpuMI RGGWCCY 2 cut(s) 790, 1246
Psp5II RGGWCCY 2 cut(s) 790, 1246
PspFI CCCAGC 1 cut(s) 822
PspN4I GGNNCC 5 cut(s) 303, 791, 1040, 1104, 1248
PspPI GGNCC 8 cut(s) 287, 301, 431, 472, 790, 812, 1039, 1246
PspPPI RGGWCCY 2 cut(s) 790, 1246
PstI CTGCAG 1 cut(s) 23
PsuI RGATCY 1 cut(s) 631
PteI GCGCGC 1 cut(s) 899
RsaI GTAC 4 cut(s) 253, 261, 497, 656
RsaNI GTAC 4 cut(s) 252, 260, 496, 655
SalI GTCGAC 1 cut(s) 1211
SaqAI TTAA 6 cut(s) 105, 935, 939, 969, 1338, 1396
SatI GCNGC 4 cut(s) 27, 45, 114, 1050
Sau3AI GATC 6 cut(s) 180, 268, 571, 579, 585, 631
Sau96I GGNCC 8 cut(s) 287, 301, 431, 472, 790, 812, 1039, 1246
SbfI CCTGCAGG 1 cut(s) 23
SchI GAGTC 2 cut(s) 139, 861
ScrFI CCNGG 1 cut(s) 1080
SdaI CCTGCAGG 1 cut(s) 23
SduI GDGCHC 1 cut(s) 335
SfcI CTRYAG 1 cut(s) 19
SfiI GGCCNNNNNGGCC 1 cut(s) 44
SfuI TTCGAA 2 cut(s) 72, 384
SinI GGWCC 5 cut(s) 287, 301, 790, 1039, 1246
SmlI CTYRAG 2 cut(s) 147, 1301
SmoI CTYRAG 2 cut(s) 147, 1301
Sse8387I CCTGCAGG 1 cut(s) 23
Sse9I AATT 6 cut(s) 12, 607, 923, 936, 1015, 1231
SseBI AGGCCT 1 cut(s) 717
SsiI CCGC 3 cut(s) 44, 488, 883
SspMI CTAG 5 cut(s) 476, 618, 638, 797, 1152
StuI AGGCCT 1 cut(s) 717
StyD4I CCNGG 1 cut(s) 1078
StyI CCWWGG 2 cut(s) 39, 1042
TaaI ACNGT 7 cut(s) 34, 251, 287, 295, 464, 553, 1343
TaiI ACGT 1 cut(s) 1317
TaqI TCGA 5 cut(s) 72, 376, 384, 1075, 1212
TasI AATT 6 cut(s) 12, 607, 923, 936, 1015, 1231
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 3 cut(s) 160, 237, 998
Tru1I TTAA 6 cut(s) 105, 935, 939, 969, 1338, 1396
Tru9I TTAA 6 cut(s) 105, 935, 939, 969, 1338, 1396
TscAI CASTG 2 cut(s) 469, 490
TseFI GTSAC 1 cut(s) 855
TseI GCWGC 3 cut(s) 26, 113, 1049
Tsp45I GTSAC 1 cut(s) 855
TspRI CASTG 2 cut(s) 469, 490
Van91I CCANNNNNTGG 1 cut(s) 612
VpaK11BI GGWCC 5 cut(s) 287, 301, 790, 1039, 1246
XapI RAATTY 2 cut(s) 1015, 1231
XcmI CCANNNNNNNNNTGG 2 cut(s) 886, 1031
XmiI GTMKAC 1 cut(s) 1212
XspI CTAG 5 cut(s) 476, 618, 638, 797, 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.