RLG00000006317

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001444
Physical Location & Seq
Reverse (-)
6212419 .. 6214365
1947 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006317

Sequence Viewer

Length: 1512 bp
ATGTTTTTACGCATCCAATCGAGTATTAACCCATCTTCTTCTCTACACCTCCAGCAATCTTCATGTATTTTTACCTTCCGGGGCACAATGCATGGGGGCCTTTGTTCATCATATATTGAAGTTAAGCAGATCCGAATACCAGATCGAAGGCAAATGAGGAAGATGAAGAAGAGGGTTTTGGTAGGATTGGCTTCTTTGTGCTTGTGTTTCATTGTATTCCATATCAGTGTCTCATTAACCTCCAGATTTCCTAAGAGTGCAGCATTTTCAGCAGAGACTCGAGAAATAACACGTGGGATATTGGAAATTCATGAACAAATCCATCACCCTCCACAGAAAAGTCCGATCATTTGCCACCGCACCAGTAAACATTACGATATCTGCTCGATCAACGGCCCAACAGTCTTGGATCCAACCACTTCCACATTCTTCACTATGGACTCATCCCAACTTGCATCCCTGGAAGTGGAAAGAATCAGACCTTACCCTCGAAAATTCGAAGATTTCATAATGGCCCAGATCAAGGACCTCACACTTGCTGTTGGCCCACGAAGCCCACCGTGTAAAGTCCAACACAATGCTCCTGCCCTAGTATTCGCTGCCGGTGGATACACCGGAAACTTTTGGCATGACTTCAATGATGGATTTATCCCGCTCTTTATCACCGCCAACACACACTTCCCAGATCAAGACTTCGTGATCGTGGTTTCCCAAGCTCCAGGGTGGTGGGTTTATAAGTATGCCGATTTACTACGCATATTCACCAAGCACCCTATTATTACCATGGGAAATGATGCCACCACCCATTGTTTTCCTTCTGCCAATCTAGGCCTTATATCACATGGTTTCATGACCATAAACCAAACACTAATACCTCGTGCAAAAACGTTTCTGCATTTTCGTGGGCTCCTAGACAAAGCATATAAGCGAAGGTCCCAACCTCAAATTCTCCCCTCTGAGCGGACAAAACCCCGGCCACGCCTTGTTTTGGCGAGCCGGAATCATGCAACTGGACGCAGGATCGTTAACCAGGTTCAAGTAGTTCGTTTGGTAAAGAAAGTGGGGTTTGATGTGATTGTGTTTGAGCCTAAGGCCAATAACTCATTGCATGAGTCTTATGCGTTACTTAATTCAAGCCATGCTTTTGTTGGGGTACATGGCGCTGCACTTACACACTCATTGTTCCTCCGGCCGGGTTCAGTGTTCGTACAGGTCGTGCCGATTGGGGTGGACTGGGCGGCGAATGCGTGTTTCGGGAGAGTGGCGAAGGGGCTGAGGTTGGAGTATTTTGAGTACAAGATCGGAGTGAAAGAGAGTAGCTTGGTGGACAAGTATGGTAATGATAGTTTGGTGGTAAAAGATCCATTTGGTCTTCAGAAAATGGGGTGGCGTGCTGAGGTTATGGACATTTACTTGAAGGAGCAGAATGTGAAGCTGGATTTGGTTAGGTTCAAGGGGTATTTGAAGCAAGCGTTTAAGAAGGCTAAGAAATTTATGGACAACAATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

504

Amino Acids

56.89

Weight (kDa)

9.84

Isoelectric Point (pI)

42.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 293 - 403 4.6e-14 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 735
AccBSI CCGCTC 2 cut(s) 655, 961
AciI CCGC 5 cut(s) 358, 653, 666, 961, 1238
AclI AACGTT 1 cut(s) 887
AclWI GGATC 5 cut(s) 124, 404, 417, 1028, 1355
AcoI YGGCCR 2 cut(s) 974, 1190
AcsI RAATTY 4 cut(s) 306, 494, 945, 1490
AcuI CTGAAG 1 cut(s) 1358
AcvI CACGTG 1 cut(s) 293
AfaI GTAC 3 cut(s) 1155, 1209, 1295
AfiI CCNNNNNNNGG 5 cut(s) 466, 523, 960, 988, 1192
AflIII ACRYGT 1 cut(s) 290
AgsI TTSAA 7 cut(s) 119, 637, 1037, 1134, 1417, 1453, 1465
AjnI CCWGG 3 cut(s) 459, 718, 1029
AjuI GAANNNNNNNTTGG 8 cut(s) 161, 193, 662, 694, 930, 962, 1424, 1456
AluBI AGCT 3 cut(s) 716, 1320, 1435
AluI AGCT 3 cut(s) 716, 1320, 1435
Alw26I GTCTC 2 cut(s) 235, 269
AlwI GGATC 5 cut(s) 124, 404, 417, 1028, 1355
Ama87I CYCGRG 1 cut(s) 279
AoxI GGCC 8 cut(s) 97, 394, 513, 544, 829, 974, 1092, 1190
ApeKI GCWGC 3 cut(s) 260, 599, 1163
ApoI RAATTY 4 cut(s) 306, 494, 945, 1490
AspLEI GCGC 1 cut(s) 1163
AspS9I GGNCC 6 cut(s) 97, 395, 514, 526, 545, 933
AsuC2I CCSGG 3 cut(s) 80, 973, 1194
AsuHPI GGTGA 3 cut(s) 317, 655, 754
AsuII TTCGAA 1 cut(s) 498
AvaI CYCGRG 1 cut(s) 279
AvaII GGWCC 2 cut(s) 526, 933
AxyI CCTNAGG 1 cut(s) 1089
BaeGI GKGCMC 1 cut(s) 86
BamHI GGATCC 1 cut(s) 409
BanII GRGCYC 1 cut(s) 909
BauI CACGAG 1 cut(s) 876
BbrPI CACGTG 1 cut(s) 293
BbsI GAAGAC 1 cut(s) 1364
BbvCI CCTCAGC 2 cut(s) 1274, 1395
BbvI GCAGC 3 cut(s) 272, 586, 1150
BccI CCATC 3 cut(s) 40, 330, 635
BceAI ACGGC 1 cut(s) 409
BciT130I CCWGG 3 cut(s) 461, 720, 1031
BciVI GTATCC 1 cut(s) 602
BcnI CCSGG 3 cut(s) 80, 973, 1194
BcoDI GTCTC 2 cut(s) 235, 269
BfaI CTAG 3 cut(s) 590, 827, 911
BfoI RGCGCY 1 cut(s) 1164
BfuI GTATCC 1 cut(s) 602
BisI GCNGC 4 cut(s) 261, 600, 1164, 1239
BlsI GCNGC 4 cut(s) 262, 601, 1165, 1240
Bme1390I CCNGG 6 cut(s) 80, 461, 720, 973, 1031, 1194
Bme18I GGWCC 2 cut(s) 526, 933
BmeT110I CYCGRG 1 cut(s) 279
BmgT120I GGNCC 6 cut(s) 97, 395, 514, 526, 545, 933
BmiI GGNNCC 4 cut(s) 98, 411, 908, 935
BmrFI CCNGG 6 cut(s) 80, 461, 720, 973, 1031, 1194
BmrI ACTGGG 1 cut(s) 1243
BmsI GCATC 3 cut(s) 21, 464, 784
BmuI ACTGGG 1 cut(s) 1243
BpiI GAAGAC 1 cut(s) 1364
BpmI CTGGAG 3 cut(s) 35, 226, 702
Bpu10I CCTNAGC 2 cut(s) 1274, 1395
Bpu14I TTCGAA 1 cut(s) 498
BpuMI CCSGG 3 cut(s) 80, 973, 1194
BsaAI YACGTR 1 cut(s) 293
BsaJI CCNNGG 5 cut(s) 79, 459, 719, 783, 971
BsaWI WCCGGW 1 cut(s) 614
Bsc4I CCNNNNNNNGG 5 cut(s) 466, 523, 960, 988, 1192
Bse118I RCCGGY 1 cut(s) 602
Bse1I ACTGG 3 cut(s) 363, 1015, 1238
Bse21I CCTNAGG 1 cut(s) 1089
Bse3DI GCAATG 1 cut(s) 1103
BseBI CCWGG 3 cut(s) 461, 720, 1031
BseDI CCNNGG 5 cut(s) 79, 459, 719, 783, 971
BseGI GGATG 3 cut(s) 12, 443, 455
BseLI CCNNNNNNNGG 5 cut(s) 466, 523, 960, 988, 1192
BseMI GCAATG 1 cut(s) 1103
BseMII CTCAG 3 cut(s) 948, 1265, 1386
BseNI ACTGG 3 cut(s) 363, 1015, 1238
BseSI GKGCMC 1 cut(s) 86
BseX3I CGGCCG 1 cut(s) 1190
BseXI GCAGC 3 cut(s) 272, 586, 1150
BsgI GTGCAG 2 cut(s) 279, 1149
Bsh1285I CGRYCG 1 cut(s) 1193
BshFI GGCC 8 cut(s) 99, 396, 515, 546, 831, 976, 1094, 1192
BsiEI CGRYCG 1 cut(s) 1193
BsiHKCI CYCGRG 1 cut(s) 279
BsiSI CCGG 7 cut(s) 79, 603, 615, 973, 997, 1189, 1193
BslFI GGGAC 1 cut(s) 919
BslI CCNNNNNNNGG 5 cut(s) 466, 523, 960, 988, 1192
BsmAI GTCTC 2 cut(s) 235, 269
BsmFI GGGAC 1 cut(s) 919
BsmI GAATGC 1 cut(s) 1249
BsnI GGCC 8 cut(s) 99, 396, 515, 546, 831, 976, 1094, 1192
BsoBI CYCGRG 1 cut(s) 279
Bsp119I TTCGAA 1 cut(s) 498
Bsp1286I GDGCHC 2 cut(s) 86, 909
Bsp19I CCATGG 1 cut(s) 783
BspACI CCGC 5 cut(s) 358, 653, 666, 961, 1238
BspANI GGCC 8 cut(s) 99, 396, 515, 546, 831, 976, 1094, 1192
BspCNI CTCAG 3 cut(s) 949, 1266, 1387
BspHI TCATGA 2 cut(s) 310, 849
BspLI GGNNCC 4 cut(s) 98, 411, 908, 935
BspPI GGATC 5 cut(s) 124, 404, 417, 1028, 1355
BspT104I TTCGAA 1 cut(s) 498
BsrBI CCGCTC 2 cut(s) 655, 961
BsrDI GCAATG 1 cut(s) 1103
BsrFI RCCGGY 1 cut(s) 602
BsrI ACTGG 3 cut(s) 363, 1015, 1238
BssAI RCCGGY 1 cut(s) 602
BssECI CCNNGG 5 cut(s) 79, 459, 719, 783, 971
BssSI CACGAG 1 cut(s) 876
BssT1I CCWWGG 1 cut(s) 783
Bst2BI CACGAG 1 cut(s) 876
Bst2UI CCWGG 3 cut(s) 461, 720, 1031
Bst4CI ACNGT 2 cut(s) 403, 561
Bst6I CTCTTC 1 cut(s) 164
BstBAI YACGTR 1 cut(s) 293
BstBI TTCGAA 1 cut(s) 498
BstC8I GCNNGC 3 cut(s) 994, 1392, 1470
BstDEI CTNAG 6 cut(s) 252, 957, 1089, 1274, 1395, 1485
BstDSI CCRYGG 1 cut(s) 783
BstF5I GGATG 3 cut(s) 12, 443, 455
BstH2I RGCGCY 1 cut(s) 1164
BstHHI GCGC 1 cut(s) 1163
BstMAI GTCTC 2 cut(s) 235, 269
BstMCI CGRYCG 1 cut(s) 1193
BstMWI GCNNNNNNNGC 3 cut(s) 269, 552, 1244
BstNI CCWGG 3 cut(s) 461, 720, 1031
BstSCI CCNGG 6 cut(s) 78, 459, 718, 971, 1029, 1192
BstSLI GKGCMC 1 cut(s) 86
BstV1I GCAGC 3 cut(s) 272, 586, 1150
BstV2I GAAGAC 1 cut(s) 1364
BstX2I RGATCY 3 cut(s) 129, 409, 1360
BstXI CCANNNNNNTGG 1 cut(s) 726
BstYI RGATCY 3 cut(s) 129, 409, 1360
BstZI CGGCCG 1 cut(s) 1190
Bsu36I CCTNAGG 1 cut(s) 1089
BsuI GTATCC 1 cut(s) 602
BsuRI GGCC 8 cut(s) 99, 396, 515, 546, 831, 976, 1094, 1192
BtgI CCRYGG 1 cut(s) 783
BtsCI GGATG 3 cut(s) 12, 443, 455
BtsIMutI CAGTG 2 cut(s) 232, 1206
Cac8I GCNNGC 3 cut(s) 994, 1392, 1470
CciI TCATGA 2 cut(s) 310, 849
CfoI GCGC 1 cut(s) 1163
Cfr10I RCCGGY 1 cut(s) 602
Cfr13I GGNCC 6 cut(s) 97, 395, 514, 526, 545, 933
CseI GACGC 1 cut(s) 1023
CsiI ACCWGGT 1 cut(s) 1029
Csp6I GTAC 3 cut(s) 1154, 1208, 1294
CviQI GTAC 3 cut(s) 1154, 1208, 1294
DdeI CTNAG 6 cut(s) 252, 957, 1089, 1274, 1395, 1485
EaeI YGGCCR 2 cut(s) 974, 1190
EagI CGGCCG 1 cut(s) 1190
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
EclXI CGGCCG 1 cut(s) 1190
Eco130I CCWWGG 1 cut(s) 783
Eco147I AGGCCT 1 cut(s) 831
Eco24I GRGCYC 1 cut(s) 909
Eco32I GATATC 1 cut(s) 379
Eco47I GGWCC 2 cut(s) 526, 933
Eco52I CGGCCG 1 cut(s) 1190
Eco57I CTGAAG 1 cut(s) 1358
Eco72I CACGTG 1 cut(s) 293
Eco81I CCTNAGG 1 cut(s) 1089
Eco88I CYCGRG 1 cut(s) 279
EcoO109I RGGNCCY 3 cut(s) 97, 526, 933
EcoRII CCWGG 3 cut(s) 459, 718, 1029
EcoRV GATATC 1 cut(s) 379
EcoT14I CCWWGG 1 cut(s) 783
EcoT22I ATGCAT 1 cut(s) 93
EcoT38I GRGCYC 1 cut(s) 909
ErhI CCWWGG 1 cut(s) 783
FalI AAGNNNNNCTT 2 cut(s) 1126, 1158
FaqI GGGAC 1 cut(s) 919
FauI CCCGC 1 cut(s) 660
Fnu4HI GCNGC 4 cut(s) 261, 600, 1164, 1239
FokI GGATG 2 cut(s) 430, 442
FriOI GRGCYC 1 cut(s) 909
Fsp4HI GCNGC 4 cut(s) 261, 600, 1164, 1239
FspBI CTAG 3 cut(s) 590, 827, 911
GlaI GCGC 1 cut(s) 1162
GluI GCNGC 4 cut(s) 261, 600, 1164, 1239
GsuI CTGGAG 3 cut(s) 35, 226, 702
HaeII RGCGCY 1 cut(s) 1164
HaeIII GGCC 8 cut(s) 99, 396, 515, 546, 831, 976, 1094, 1192
HapII CCGG 7 cut(s) 79, 603, 615, 973, 997, 1189, 1193
HgaI GACGC 1 cut(s) 1023
HhaI GCGC 1 cut(s) 1163
Hin6I GCGC 1 cut(s) 1161
HinP1I GCGC 1 cut(s) 1161
HincII GTYRAC 1 cut(s) 1027
HindII GTYRAC 1 cut(s) 1027
HinfI GANTC 5 cut(s) 277, 440, 474, 1000, 1112
HpaI GTTAAC 1 cut(s) 1027
HpaII CCGG 7 cut(s) 79, 603, 615, 973, 997, 1189, 1193
HphI GGTGA 3 cut(s) 317, 655, 754
Hpy166II GTNNAC 4 cut(s) 368, 1027, 1231, 1327
Hpy188I TCNGA 6 cut(s) 134, 345, 479, 958, 1304, 1377
Hpy188III TCNNGA 7 cut(s) 243, 281, 311, 689, 697, 850, 1255
Hpy8I GTNNAC 4 cut(s) 368, 1027, 1231, 1327
HpyAV CCTTC 7 cut(s) 85, 141, 825, 924, 1261, 1411, 1474
HpyCH4III ACNGT 2 cut(s) 403, 561
HpyCH4IV ACGT 2 cut(s) 292, 887
HpyCH4V TGCA 8 cut(s) 91, 260, 455, 881, 895, 1007, 1108, 1166
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 552, 1244
HpyF3I CTNAG 6 cut(s) 252, 957, 1089, 1274, 1395, 1485
HpySE526I ACGT 2 cut(s) 292, 887
HspAI GCGC 1 cut(s) 1161
KspAI GTTAAC 1 cut(s) 1027
LmnI GCTCC 4 cut(s) 586, 721, 912, 1420
Lsp1109I GCAGC 3 cut(s) 272, 586, 1150
LweI GCATC 3 cut(s) 21, 464, 784
MabI ACCWGGT 1 cut(s) 1029
MaeI CTAG 3 cut(s) 590, 827, 911
MaeII ACGT 2 cut(s) 292, 887
MaeIII GTNAC 1 cut(s) 1122
MbiI CCGCTC 2 cut(s) 655, 961
MboII GAAGA 9 cut(s) 27, 30, 51, 172, 178, 181, 421, 512, 1364
MflI RGATCY 3 cut(s) 129, 409, 1360
MhlI GDGCHC 2 cut(s) 86, 909
MluCI AATT 5 cut(s) 306, 494, 945, 1129, 1490
MlyI GAGTC 3 cut(s) 271, 434, 1121
MmeI TCCRAC 3 cut(s) 437, 595, 1260
Mph1103I ATGCAT 1 cut(s) 93
MseI TTAA 6 cut(s) 27, 123, 236, 1026, 1128, 1476
MslI CAYNNNNRTG 2 cut(s) 225, 900
MspI CCGG 7 cut(s) 79, 603, 615, 973, 997, 1189, 1193
MspR9I CCNGG 6 cut(s) 80, 461, 720, 973, 1031, 1194
Mva1269I GAATGC 1 cut(s) 1249
MvaI CCWGG 3 cut(s) 461, 720, 1031
MwoI GCNNNNNNNGC 3 cut(s) 269, 552, 1244
NciI CCSGG 3 cut(s) 80, 973, 1194
NcoI CCATGG 1 cut(s) 783
NlaIV GGNNCC 4 cut(s) 98, 411, 908, 935
NsiI ATGCAT 1 cut(s) 93
NspV TTCGAA 1 cut(s) 498
PaeR7I CTCGAG 1 cut(s) 279
PagI TCATGA 2 cut(s) 310, 849
PceI AGGCCT 1 cut(s) 831
PcsI WCGNNNNNNNCGW 1 cut(s) 1212
PctI GAATGC 1 cut(s) 1249
PfeI GAWTC 2 cut(s) 474, 1000
PkrI GCNGC 4 cut(s) 262, 601, 1165, 1240
PleI GAGTC 3 cut(s) 271, 434, 1120
PmaCI CACGTG 1 cut(s) 293
PmlI CACGTG 1 cut(s) 293
PpsI GAGTC 3 cut(s) 271, 434, 1120
Ppu21I YACGTR 1 cut(s) 293
PpuMI RGGWCCY 2 cut(s) 526, 933
PsiI TTATAA 1 cut(s) 735
Psp1406I AACGTT 1 cut(s) 887
Psp5II RGGWCCY 2 cut(s) 526, 933
Psp6I CCWGG 3 cut(s) 459, 718, 1029
PspCI CACGTG 1 cut(s) 293
PspGI CCWGG 3 cut(s) 459, 718, 1029
PspN4I GGNNCC 4 cut(s) 98, 411, 908, 935
PspPI GGNCC 6 cut(s) 97, 395, 514, 526, 545, 933
PspPPI RGGWCCY 2 cut(s) 526, 933
PsuI RGATCY 3 cut(s) 129, 409, 1360
RsaI GTAC 3 cut(s) 1155, 1209, 1295
RsaNI GTAC 3 cut(s) 1154, 1208, 1294
RseI CAYNNNNRTG 2 cut(s) 225, 900
SaqAI TTAA 6 cut(s) 27, 123, 236, 1026, 1128, 1476
SatI GCNGC 4 cut(s) 261, 600, 1164, 1239
Sau96I GGNCC 6 cut(s) 97, 395, 514, 526, 545, 933
SchI GAGTC 3 cut(s) 271, 434, 1121
ScrFI CCNGG 6 cut(s) 80, 461, 720, 973, 1031, 1194
SduI GDGCHC 2 cut(s) 86, 909
SexAI ACCWGGT 1 cut(s) 1029
SfaNI GCATC 3 cut(s) 21, 464, 784
Sfr274I CTCGAG 1 cut(s) 279
SfuI TTCGAA 1 cut(s) 498
SinI GGWCC 2 cut(s) 526, 933
SlaI CTCGAG 1 cut(s) 279
SmiMI CAYNNNNRTG 2 cut(s) 225, 900
SmlI CTYRAG 1 cut(s) 279
SmoI CTYRAG 1 cut(s) 279
Sse9I AATT 5 cut(s) 306, 494, 945, 1129, 1490
SseBI AGGCCT 1 cut(s) 831
SsiI CCGC 5 cut(s) 358, 653, 666, 961, 1238
SspMI CTAG 3 cut(s) 590, 827, 911
StuI AGGCCT 1 cut(s) 831
StyD4I CCNGG 6 cut(s) 78, 459, 718, 971, 1029, 1192
StyI CCWWGG 1 cut(s) 783
TaaI ACNGT 2 cut(s) 403, 561
TaiI ACGT 2 cut(s) 295, 890
TaqI TCGA 6 cut(s) 20, 145, 280, 386, 490, 498
TasI AATT 5 cut(s) 306, 494, 945, 1129, 1490
TatI WGTACW 1 cut(s) 1293
TauI GCSGC 1 cut(s) 1241
TfiI GAWTC 2 cut(s) 474, 1000
Tru1I TTAA 6 cut(s) 27, 123, 236, 1026, 1128, 1476
Tru9I TTAA 6 cut(s) 27, 123, 236, 1026, 1128, 1476
TscAI CASTG 2 cut(s) 232, 1206
TseI GCWGC 3 cut(s) 260, 599, 1163
TspDTI ATGAA 8 cut(s) 51, 96, 179, 199, 299, 327, 496, 838
TspRI CASTG 2 cut(s) 232, 1206
VpaK11BI GGWCC 2 cut(s) 526, 933
XapI RAATTY 4 cut(s) 306, 494, 945, 1490
XcmI CCANNNNNNNNNTGG 1 cut(s) 1145
XhoI CTCGAG 1 cut(s) 279
XspI CTAG 3 cut(s) 590, 827, 911
Zsp2I ATGCAT 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.