RchiOBHm_Chr4g0438911

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001444
Physical Location & Seq
Forward (+)
61442658 .. 61443974
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40699

Sequence Viewer

Length: 1317 bp
ATGTACATTATTTTAGATATTTCAAGGTGCATTAACCTTCAACATAAATGTTTCTTGTTAATGGTTGTAGATATCTTCAAACGCTTAGCACCAAGATCAAATACTAGCGGCCGGAACCCTATCAATTCGACACGTTTGCCATCTTCGACATCAACAAAGATTACATGCACCCGTTCTCACAAATACTACGATTTCTGTTTCATCGACGGCCCAACAGTTCTGGACCCCACAACCTCCACGTTATTTGTAATGGACTCCACGGGCCCAACCTTGGTGGAAAAAATCAAGCCCTACCCACGAAAACATGAGAATTCGACAATGTCGAGAATCAAAGAGCTCACCCTAACATCAGGCCCACCAGGCCCAAAATGCCAGGTCCAACACAATGCCCCGGCAATTGTATTCAGTGCGGGCGGGTACACCGGAAACTTCTTCCATGATTTCAATGATGGGTTCATCCCCCTCTACATCATAGTCAATTCGATTCTTTACGATCAAGATCCCGTGCTCGTGATTTCCAAAGCACGTGATTGGTGGCTTCAGAGGTACTCAGATTTGCTACAAGTTTTTAGCGAGCACCCAATTATAAACCTTGATAATGATACTGCCACCCATTGTTTTCGTTCTGCCACCGTGGGTCTAATTTCTCATGGATTTTTGACCATAAACCCCAAATCGTTAAAGACCCCCAAGACACTGCCTCATTTCCATGCCATCCTAGGCAAAGCCTTTGGCCAAACTGCCCATCGCTTCTACCGTCAGCGCCACCACAATGAGCGGCCACGCATTGTGTTAATTAGTCGAACCGTAGGCGTCGGCCGAGCAATCTTGAATCAAGAGGAAGCGATGGAAGTGGCAGACAGAATAGGGTTCGAGGTGATTGTTTATGAGCCAAGTTCAAGGACCGATTTGCATGTAGCATATGACTTGCTTAGTAATAGCCATGCAGTGGTTGGTGTTCATGGTGCGGCCCTTACTCACTTGTTGTTCATGCGGCCTGGGACGGTGTTCATGCAAATTGTGCCGCTAGGGACTAATTGGTTGGCAAAAGTGTGCTATGAAAATCCAGGTAGGGCAATGGGGCTGGAGTACATTGACTATAGGATTACTGCGGAGGAGAGCAGCTTGTTAGAAACTTACGGAAAGGATGACATTGTGGTTAAGAATCCGGGAGCTAAGATTGGTACTAATTGGTCCATGGAGATTATGAAGATTTATCTCAAGGAACAGAATGTCAGGTTGGATTTGGATAGGTTTTCTTGGTTTTTGAAGGCCGCTTATGACAAGGCCAAGAAGTTTATGGACAAGGAAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

438

Amino Acids

49.65

Weight (kDa)

8.97

Isoelectric Point (pI)

25.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 160 - 337 2.3e-14 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 587
AccB7I CCANNNNNTGG 1 cut(s) 949
AccBSI CCGCTC 1 cut(s) 778
AciI CCGC 9 cut(s) 108, 410, 414, 778, 968, 994, 1025, 1112, 1275
AclWI GGATC 1 cut(s) 494
AcoI YGGCCR 4 cut(s) 109, 733, 779, 817
AcsI RAATTY 1 cut(s) 310
AcuI CTGAAG 1 cut(s) 524
AcvI CACGTG 1 cut(s) 527
AcyI GRCGYC 1 cut(s) 813
AfaI GTAC 5 cut(s) 5, 419, 548, 1091, 1186
AfiI CCNNNNNNNGG 2 cut(s) 271, 949
AflIII ACRYGT 1 cut(s) 131
AgsI TTSAA 7 cut(s) 24, 41, 79, 445, 832, 900, 1270
AjnI CCWGG 4 cut(s) 358, 372, 997, 1066
AjuI GAANNNNNNNTTGG 2 cut(s) 1223, 1255
AluBI AGCT 3 cut(s) 337, 1125, 1175
AluI AGCT 3 cut(s) 337, 1125, 1175
Alw21I GWGCWC 3 cut(s) 339, 510, 579
AlwI GGATC 1 cut(s) 494
ApaI GGGCCC 1 cut(s) 266
ApeKI GCWGC 1 cut(s) 1122
ApoI RAATTY 1 cut(s) 310
AspA2I CCTAGG 1 cut(s) 718
AspLEI GCGC 1 cut(s) 765
AsuC2I CCSGG 2 cut(s) 392, 1170
AsuHPI GGTGA 2 cut(s) 331, 889
AvaII GGWCC 4 cut(s) 223, 376, 903, 1194
AvrII CCTAGG 1 cut(s) 718
BaeGI GKGCMC 1 cut(s) 266
BalI TGGCCA 1 cut(s) 735
BanII GRGCYC 2 cut(s) 266, 339
BauI CACGAG 1 cut(s) 509
BbrPI CACGTG 1 cut(s) 527
Bbv12I GWGCWC 3 cut(s) 339, 510, 579
BbvI GCAGC 1 cut(s) 1134
BccI CCATC 5 cut(s) 148, 443, 722, 753, 841
BceAI ACGGC 1 cut(s) 223
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 4 cut(s) 360, 374, 999, 1068
BcnI CCSGG 2 cut(s) 392, 1170
BfaI CTAG 3 cut(s) 105, 719, 1028
BfmI CTRYAG 1 cut(s) 1099
BfoI RGCGCY 1 cut(s) 766
BglI GCCNNNNNGGC 1 cut(s) 360
BisI GCNGC 7 cut(s) 109, 779, 969, 995, 1025, 1123, 1275
BlnI CCTAGG 1 cut(s) 718
BlpI GCTNAGC 1 cut(s) 85
BlsI GCNGC 7 cut(s) 110, 780, 970, 996, 1026, 1124, 1276
Bme1390I CCNGG 6 cut(s) 360, 374, 392, 999, 1068, 1170
Bme18I GGWCC 4 cut(s) 223, 376, 903, 1194
BmiI GGNNCC 3 cut(s) 116, 225, 264
BmrFI CCNGG 6 cut(s) 360, 374, 392, 999, 1068, 1170
BpmI CTGGAG 1 cut(s) 1106
Bpu1102I GCTNAGC 1 cut(s) 85
BpuEI CTTGAG 1 cut(s) 1205
BpuMI CCSGG 2 cut(s) 392, 1170
BsaAI YACGTR 1 cut(s) 527
BsaBI GATNNNNATC 1 cut(s) 498
BsaHI GRCGYC 1 cut(s) 813
BsaJI CCNNGG 7 cut(s) 258, 270, 390, 633, 718, 998, 1197
BsaWI WCCGGW 1 cut(s) 422
Bsc4I CCNNNNNNNGG 2 cut(s) 271, 949
Bse3DI GCAATG 1 cut(s) 1083
Bse8I GATNNNNATC 1 cut(s) 498
BseBI CCWGG 4 cut(s) 360, 374, 999, 1068
BseDI CCNNGG 7 cut(s) 258, 270, 390, 633, 718, 998, 1197
BseGI GGATG 3 cut(s) 456, 714, 1153
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 2 cut(s) 271, 949
BseMI GCAATG 1 cut(s) 1083
BseMII CTCAG 1 cut(s) 564
BseRI GAGGAG 1 cut(s) 1130
BseSI GKGCMC 1 cut(s) 266
BseX3I CGGCCG 2 cut(s) 109, 817
BseXI GCAGC 1 cut(s) 1134
Bsh1285I CGRYCG 2 cut(s) 112, 820
BsiEI CGRYCG 2 cut(s) 112, 820
BsiHKAI GWGCWC 3 cut(s) 339, 510, 579
BsiSI CCGG 4 cut(s) 112, 392, 423, 1169
BslFI GGGAC 2 cut(s) 1015, 1045
BslI CCNNNNNNNGG 2 cut(s) 271, 949
BsmFI GGGAC 2 cut(s) 1015, 1045
Bsp120I GGGCCC 1 cut(s) 262
Bsp1286I GDGCHC 4 cut(s) 266, 339, 510, 579
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 95, 493, 499
Bsp1720I GCTNAGC 1 cut(s) 85
Bsp19I CCATGG 1 cut(s) 1197
BspACI CCGC 9 cut(s) 108, 410, 414, 778, 968, 994, 1025, 1112, 1275
BspCNI CTCAG 1 cut(s) 563
BspLI GGNNCC 3 cut(s) 116, 225, 264
BspPI GGATC 1 cut(s) 494
BsrBI CCGCTC 1 cut(s) 778
BsrDI GCAATG 1 cut(s) 1083
BsrGI TGTACA 1 cut(s) 3
BssECI CCNNGG 7 cut(s) 258, 270, 390, 633, 718, 998, 1197
BssMI GATC 3 cut(s) 95, 493, 499
BssNI GRCGYC 1 cut(s) 813
BssSI CACGAG 1 cut(s) 509
BssT1I CCWWGG 3 cut(s) 270, 718, 1197
Bst2BI CACGAG 1 cut(s) 509
Bst2UI CCWGG 4 cut(s) 360, 374, 999, 1068
Bst4CI ACNGT 5 cut(s) 217, 634, 758, 808, 1006
BstACI GRCGYC 1 cut(s) 813
BstAPI GCANNNNNTGC 1 cut(s) 1021
BstAUI TGTACA 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 527
BstC8I GCNNGC 2 cut(s) 412, 575
BstDEI CTNAG 4 cut(s) 85, 550, 932, 1176
BstDSI CCRYGG 3 cut(s) 258, 633, 1197
BstF5I GGATG 3 cut(s) 456, 714, 1153
BstH2I RGCGCY 1 cut(s) 766
BstHHI GCGC 1 cut(s) 765
BstKTI GATC 3 cut(s) 98, 496, 502
BstMBI GATC 3 cut(s) 95, 493, 499
BstMCI CGRYCG 2 cut(s) 112, 820
BstMWI GCNNNNNNNGC 3 cut(s) 360, 369, 1021
BstNI CCWGG 4 cut(s) 360, 374, 999, 1068
BstNSI RCATGY 2 cut(s) 168, 917
BstSCI CCNGG 6 cut(s) 358, 372, 390, 997, 1066, 1168
BstSFI CTRYAG 1 cut(s) 1099
BstSLI GKGCMC 1 cut(s) 266
BstV1I GCAGC 1 cut(s) 1134
BstX2I RGATCY 1 cut(s) 499
BstYI RGATCY 1 cut(s) 499
BstZI CGGCCG 2 cut(s) 109, 817
BtgI CCRYGG 3 cut(s) 258, 633, 1197
BtgZI GCGATG 2 cut(s) 731, 860
BtsCI GGATG 3 cut(s) 456, 714, 1153
BtsI GCAGTG 2 cut(s) 695, 954
BtsIMutI CAGTG 3 cut(s) 412, 695, 954
Cac8I GCNNGC 2 cut(s) 412, 575
CfoI GCGC 1 cut(s) 765
CseI GACGC 1 cut(s) 802
Csp6I GTAC 5 cut(s) 4, 418, 547, 1090, 1185
CspCI CAANNNNNGTGG 4 cut(s) 226, 255, 261, 290
CviQI GTAC 5 cut(s) 4, 418, 547, 1090, 1185
DdeI CTNAG 4 cut(s) 85, 550, 932, 1176
DpnI GATC 3 cut(s) 97, 495, 501
DpnII GATC 3 cut(s) 95, 493, 499
EaeI YGGCCR 4 cut(s) 109, 733, 779, 817
EagI CGGCCG 2 cut(s) 109, 817
Ecl136II GAGCTC 1 cut(s) 337
EclXI CGGCCG 2 cut(s) 109, 817
Eco130I CCWWGG 3 cut(s) 270, 718, 1197
Eco24I GRGCYC 2 cut(s) 266, 339
Eco32I GATATC 1 cut(s) 73
Eco47I GGWCC 4 cut(s) 223, 376, 903, 1194
Eco52I CGGCCG 2 cut(s) 109, 817
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 1 cut(s) 524
Eco72I CACGTG 1 cut(s) 527
EcoICRI GAGCTC 1 cut(s) 337
EcoRI GAATTC 1 cut(s) 310
EcoRII CCWGG 4 cut(s) 358, 372, 997, 1066
EcoRV GATATC 1 cut(s) 73
EcoT14I CCWWGG 3 cut(s) 270, 718, 1197
EcoT38I GRGCYC 2 cut(s) 266, 339
ErhI CCWWGG 3 cut(s) 270, 718, 1197
FaqI GGGAC 2 cut(s) 1015, 1045
FauI CCCGC 2 cut(s) 403, 407
FauNDI CATATG 1 cut(s) 922
Fnu4HI GCNGC 7 cut(s) 109, 779, 969, 995, 1025, 1123, 1275
FokI GGATG 3 cut(s) 443, 701, 1160
FriOI GRGCYC 2 cut(s) 266, 339
Fsp4HI GCNGC 7 cut(s) 109, 779, 969, 995, 1025, 1123, 1275
FspBI CTAG 3 cut(s) 105, 719, 1028
GlaI GCGC 1 cut(s) 764
GluI GCNGC 7 cut(s) 109, 779, 969, 995, 1025, 1123, 1275
GsuI CTGGAG 1 cut(s) 1106
HaeII RGCGCY 1 cut(s) 766
HapII CCGG 4 cut(s) 112, 392, 423, 1169
HgaI GACGC 1 cut(s) 802
HhaI GCGC 1 cut(s) 765
Hin1I GRCGYC 1 cut(s) 813
Hin6I GCGC 1 cut(s) 763
HinP1I GCGC 1 cut(s) 763
HinfI GANTC 5 cut(s) 254, 327, 484, 832, 1165
HpaII CCGG 4 cut(s) 112, 392, 423, 1169
HphI GGTGA 2 cut(s) 331, 889
Hpy166II GTNNAC 1 cut(s) 420
Hpy188I TCNGA 2 cut(s) 543, 553
Hpy188III TCNNGA 6 cut(s) 221, 324, 497, 511, 829, 836
Hpy8I GTNNAC 1 cut(s) 420
Hpy99I CGWCG 2 cut(s) 209, 818
HpyAV CCTTC 3 cut(s) 47, 1264, 1304
HpyCH4III ACNGT 5 cut(s) 217, 634, 758, 808, 1006
HpyCH4IV ACGT 3 cut(s) 133, 239, 526
HpyCH4V TGCA 5 cut(s) 30, 168, 913, 947, 1015
HpyF10VI GCNNNNNNNGC 3 cut(s) 360, 369, 1021
HpyF3I CTNAG 4 cut(s) 85, 550, 932, 1176
HpySE526I ACGT 3 cut(s) 133, 239, 526
Hsp92I GRCGYC 1 cut(s) 813
HspAI GCGC 1 cut(s) 763
Kzo9I GATC 3 cut(s) 95, 493, 499
LmnI GCTCC 1 cut(s) 1172
Lsp1109I GCAGC 1 cut(s) 1134
MaeI CTAG 3 cut(s) 105, 719, 1028
MaeII ACGT 3 cut(s) 133, 239, 526
MalI GATC 3 cut(s) 97, 495, 501
MbiI CCGCTC 1 cut(s) 778
MboI GATC 3 cut(s) 95, 493, 499
MboII GAAGA 4 cut(s) 67, 135, 424, 1222
MfeI CAATTG 1 cut(s) 396
MflI RGATCY 1 cut(s) 499
MhlI GDGCHC 4 cut(s) 266, 339, 510, 579
MlsI TGGCCA 1 cut(s) 735
MluNI TGGCCA 1 cut(s) 735
MlyI GAGTC 1 cut(s) 248
MmeI TCCRAC 2 cut(s) 403, 1221
MnlI CCTC 7 cut(s) 244, 473, 537, 711, 832, 868, 1108
Mox20I TGGCCA 1 cut(s) 735
MscI TGGCCA 1 cut(s) 735
MseI TTAA 5 cut(s) 33, 59, 680, 794, 1161
MslI CAYNNNNRTG 2 cut(s) 708, 771
Msp20I TGGCCA 1 cut(s) 735
MspI CCGG 4 cut(s) 112, 392, 423, 1169
MspR9I CCNGG 6 cut(s) 360, 374, 392, 999, 1068, 1170
MunI CAATTG 1 cut(s) 396
MvaI CCWGG 4 cut(s) 360, 374, 999, 1068
MwoI GCNNNNNNNGC 3 cut(s) 360, 369, 1021
NciI CCSGG 2 cut(s) 392, 1170
NcoI CCATGG 1 cut(s) 1197
NdeI CATATG 1 cut(s) 922
NdeII GATC 3 cut(s) 95, 493, 499
NlaIV GGNNCC 3 cut(s) 116, 225, 264
NmeAIII GCCGAG 1 cut(s) 845
NspI RCATGY 2 cut(s) 168, 917
PcsI WCGNNNNNNNCGW 2 cut(s) 320, 754
PfeI GAWTC 4 cut(s) 327, 484, 832, 1165
PflFI GACNNNGTC 1 cut(s) 319
PflMI CCANNNNNTGG 1 cut(s) 949
PfoI TCCNGGA 1 cut(s) 1168
PkrI GCNGC 7 cut(s) 110, 780, 970, 996, 1026, 1124, 1276
PleI GAGTC 1 cut(s) 248
PmaCI CACGTG 1 cut(s) 527
PmlI CACGTG 1 cut(s) 527
PpsI GAGTC 1 cut(s) 248
Ppu21I YACGTR 1 cut(s) 527
PsiI TTATAA 1 cut(s) 587
Psp124BI GAGCTC 1 cut(s) 339
Psp6I CCWGG 4 cut(s) 358, 372, 997, 1066
PspCI CACGTG 1 cut(s) 527
PspGI CCWGG 4 cut(s) 358, 372, 997, 1066
PspN4I GGNNCC 3 cut(s) 116, 225, 264
PspOMI GGGCCC 1 cut(s) 262
PsuI RGATCY 1 cut(s) 499
PsyI GACNNNGTC 1 cut(s) 319
RsaI GTAC 5 cut(s) 5, 419, 548, 1091, 1186
RsaNI GTAC 5 cut(s) 4, 418, 547, 1090, 1185
RseI CAYNNNNRTG 2 cut(s) 708, 771
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 5 cut(s) 33, 59, 680, 794, 1161
SatI GCNGC 7 cut(s) 109, 779, 969, 995, 1025, 1123, 1275
Sau3AI GATC 3 cut(s) 95, 493, 499
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 6 cut(s) 360, 374, 392, 999, 1068, 1170
SduI GDGCHC 4 cut(s) 266, 339, 510, 579
SfcI CTRYAG 1 cut(s) 1099
SfiI GGCCNNNNNGGCC 1 cut(s) 360
SinI GGWCC 4 cut(s) 223, 376, 903, 1194
SmiMI CAYNNNNRTG 2 cut(s) 708, 771
SmlI CTYRAG 1 cut(s) 1220
SmoI CTYRAG 1 cut(s) 1220
SsiI CCGC 9 cut(s) 108, 410, 414, 778, 968, 994, 1025, 1112, 1275
SspMI CTAG 3 cut(s) 105, 719, 1028
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 6 cut(s) 358, 372, 390, 997, 1066, 1168
StyI CCWWGG 3 cut(s) 270, 718, 1197
TaaI ACNGT 5 cut(s) 217, 634, 758, 808, 1006
TaiI ACGT 3 cut(s) 136, 242, 529
TaqI TCGA 8 cut(s) 128, 146, 204, 314, 323, 482, 802, 873
TaqII GACCGA 1 cut(s) 920
TatI WGTACW 2 cut(s) 3, 1089
TauI GCSGC 6 cut(s) 111, 781, 971, 997, 1027, 1277
TfiI GAWTC 4 cut(s) 327, 484, 832, 1165
Tru1I TTAA 5 cut(s) 33, 59, 680, 794, 1161
Tru9I TTAA 5 cut(s) 33, 59, 680, 794, 1161
TscAI CASTG 3 cut(s) 412, 702, 954
TseI GCWGC 1 cut(s) 1122
TspDTI ATGAA 7 cut(s) 190, 445, 950, 979, 1000, 1074, 1223
TspGWI ACGGA 1 cut(s) 1155
TspRI CASTG 3 cut(s) 412, 702, 954
Tth111I GACNNNGTC 1 cut(s) 319
Van91I CCANNNNNTGG 1 cut(s) 949
VpaK11BI GGWCC 4 cut(s) 223, 376, 903, 1194
XapI RAATTY 1 cut(s) 310
XceI RCATGY 2 cut(s) 168, 917
XcmI CCANNNNNNNNNTGG 2 cut(s) 950, 1297
XmaJI CCTAGG 1 cut(s) 718
XspI CTAG 3 cut(s) 105, 719, 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.