Rh4BG376300

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001444
Physical Location & Seq
Forward (+)
53199078 .. 53200157
1080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG376300.1

Sequence Viewer

Length: 1080 bp
ATGGACTCATCCCAACTTGCATCCCTGGAAGTGGAAAGAATCCGACCTTACCCTCGAAAATTCGAAGATTTCGTAATGGCCCAGATCAAGGACCTCACACTCGCTGTTGGCCCACGAAGCCCACCGTGTAAAGTCCAACACAATGCTCCGGCCCTAGTATTCGCCGCCGGTGGATACACCGGAAACTTTTGGCATGACTTCAATGATGGATTTATCCCGCTCTTTATCACTGCCAACACACTCTTCCCAGATCAAGACTTCGTGATCGTGGTTTCCCAAGCTCCAAAGTGGTGGGTTTATAAGTATGCCGATTTACTACCCATATTCACCAAGCACCCTATTATTACCATGGGAAATGATGCCACCACCCATTGTTTTCCTTCTGCCAATCTAGGCCTTATATCACATGGTTTCATGACTATAAACCAAACACTAATACCTCGTGCAAAAACGTTTCTGCATTTTCGTGGGCTCCTAGACAACGCATATAAGCGAAGGTCCCAACCTCAAATTCTCTCCTCTGATCAGCAGACAAAACCCCGGCCACGCCTTGTTTTGGCGAGCCGGAATCATGCAACTGGACGCAGGATTGTTAACCAGGTTCAAGTAGTTCGTTTGGTAAAGAAAGTGGGGTTTGATGTGATTGTGTTTGAGCCTAAGGCCAATAACTCATTGCATGAGTCTTATGCGTTACTTAATTCAAGCCATGCTTTTGTTGGGGTACATGGCGCTGCACTTACACACTCATTGTTCCTCCGCCCGGGTTCAGTGTTCGTACAGGTGGTGCCGATTGGGGTGGACTGGGCGGCGAATGCATGTTTCGGGGGAGTGGCGAAGGGGCTGAGGTTGGAGTATTTTGAGTACAAGATCGGAGTGAAAGAGAGTAGCTTGCTGGACAAGTATGGTAATGATAGTTTGGTGGTAAAAGATCCATTTGGTCTTCAGAAAATGGGGTGGCGTGCTGAGGTTATGGACATTTACTTGAAGGAGCAGAATGTGAAGCTGGATTTGGTTAGGTTCAAGGGGTATTTGAAGCAAGCGTTTAACAAGGCTAAGAAATTTATGGACAACAATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

359

Amino Acids

40.39

Weight (kDa)

9.62

Isoelectric Point (pI)

32.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 84 - 259 1.9e-14 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 300
AccB1I GGYRCC 1 cut(s) 784
AccBSI CCGCTC 1 cut(s) 220
AciI CCGC 4 cut(s) 165, 218, 757, 806
AclI AACGTT 1 cut(s) 452
AclWI GGATC 1 cut(s) 923
AcoI YGGCCR 1 cut(s) 542
AcsI RAATTY 3 cut(s) 59, 510, 1058
AcuI CTGAAG 1 cut(s) 926
AfaI GTAC 3 cut(s) 723, 777, 863
AfiI CCNNNNNNNGG 4 cut(s) 31, 88, 556, 760
AgsI TTSAA 6 cut(s) 202, 605, 702, 985, 1021, 1033
AjnI CCWGG 2 cut(s) 24, 597
AjuI GAANNNNNNNTTGG 6 cut(s) 227, 259, 495, 527, 992, 1024
AluBI AGCT 3 cut(s) 281, 888, 1003
AluI AGCT 3 cut(s) 281, 888, 1003
AlwI GGATC 1 cut(s) 923
Ama87I CYCGRG 1 cut(s) 760
AoxI GGCC 6 cut(s) 78, 109, 150, 394, 542, 660
ApeKI GCWGC 1 cut(s) 731
ApoI RAATTY 3 cut(s) 59, 510, 1058
AspLEI GCGC 1 cut(s) 731
AspS9I GGNCC 5 cut(s) 79, 91, 110, 151, 498
AsuC2I CCSGG 3 cut(s) 541, 761, 762
AsuHPI GGTGA 1 cut(s) 319
AsuII TTCGAA 1 cut(s) 63
AvaI CYCGRG 1 cut(s) 760
AvaII GGWCC 2 cut(s) 91, 498
AxyI CCTNAGG 1 cut(s) 657
BanI GGYRCC 1 cut(s) 784
BanII GRGCYC 1 cut(s) 474
BauI CACGAG 1 cut(s) 441
BbsI GAAGAC 1 cut(s) 932
BbvCI CCTCAGC 2 cut(s) 842, 963
BbvI GCAGC 1 cut(s) 718
BccI CCATC 1 cut(s) 200
BciT130I CCWGG 2 cut(s) 26, 599
BciVI GTATCC 1 cut(s) 167
BclI TGATCA 1 cut(s) 523
BcnI CCSGG 3 cut(s) 541, 761, 762
BfaI CTAG 3 cut(s) 155, 392, 476
BfoI RGCGCY 1 cut(s) 732
BfuI GTATCC 1 cut(s) 167
BisI GCNGC 3 cut(s) 165, 732, 807
BlsI GCNGC 3 cut(s) 166, 733, 808
Bme1390I CCNGG 5 cut(s) 26, 541, 599, 761, 762
Bme18I GGWCC 2 cut(s) 91, 498
BmeT110I CYCGRG 1 cut(s) 760
BmgT120I GGNCC 5 cut(s) 79, 91, 110, 151, 498
BmiI GGNNCC 3 cut(s) 473, 500, 786
BmrFI CCNGG 5 cut(s) 26, 541, 599, 761, 762
BmrI ACTGGG 1 cut(s) 811
BmsI GCATC 2 cut(s) 29, 349
BmuI ACTGGG 1 cut(s) 811
BpiI GAAGAC 1 cut(s) 932
Bpu10I CCTNAGC 2 cut(s) 842, 963
Bpu14I TTCGAA 1 cut(s) 63
BpuMI CCSGG 3 cut(s) 541, 761, 762
BsaJI CCNNGG 4 cut(s) 24, 348, 539, 760
BsaWI WCCGGW 1 cut(s) 179
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 88, 556, 760
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 2 cut(s) 583, 806
Bse21I CCTNAGG 1 cut(s) 657
Bse3DI GCAATG 1 cut(s) 671
BseBI CCWGG 2 cut(s) 26, 599
BseDI CCNNGG 4 cut(s) 24, 348, 539, 760
BseGI GGATG 2 cut(s) 8, 20
BseLI CCNNNNNNNGG 4 cut(s) 31, 88, 556, 760
BseMI GCAATG 1 cut(s) 671
BseMII CTCAG 2 cut(s) 833, 954
BseNI ACTGG 2 cut(s) 583, 806
BseRI GAGGAG 1 cut(s) 508
BseXI GCAGC 1 cut(s) 718
BsgI GTGCAG 1 cut(s) 717
BshFI GGCC 6 cut(s) 80, 111, 152, 396, 544, 662
BshNI GGYRCC 1 cut(s) 784
BsiHKCI CYCGRG 1 cut(s) 760
BsiSI CCGG 6 cut(s) 149, 168, 180, 541, 565, 761
BslFI GGGAC 1 cut(s) 484
BslI CCNNNNNNNGG 4 cut(s) 31, 88, 556, 760
BsmFI GGGAC 1 cut(s) 484
BsmI GAATGC 1 cut(s) 817
BsnI GGCC 6 cut(s) 80, 111, 152, 396, 544, 662
BsoBI CYCGRG 1 cut(s) 760
Bsp119I TTCGAA 1 cut(s) 63
Bsp1286I GDGCHC 1 cut(s) 474
Bsp143I GATC 6 cut(s) 84, 250, 264, 523, 867, 928
Bsp19I CCATGG 1 cut(s) 348
BspACI CCGC 4 cut(s) 165, 218, 757, 806
BspANI GGCC 6 cut(s) 80, 111, 152, 396, 544, 662
BspCNI CTCAG 2 cut(s) 834, 955
BspHI TCATGA 1 cut(s) 414
BspLI GGNNCC 3 cut(s) 473, 500, 786
BspPI GGATC 1 cut(s) 923
BspT104I TTCGAA 1 cut(s) 63
BspT107I GGYRCC 1 cut(s) 784
BsrBI CCGCTC 1 cut(s) 220
BsrDI GCAATG 1 cut(s) 671
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 2 cut(s) 583, 806
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 4 cut(s) 24, 348, 539, 760
BssMI GATC 6 cut(s) 84, 250, 264, 523, 867, 928
BssSI CACGAG 1 cut(s) 441
BssT1I CCWWGG 1 cut(s) 348
Bst2BI CACGAG 1 cut(s) 441
Bst2UI CCWGG 2 cut(s) 26, 599
Bst4CI ACNGT 1 cut(s) 126
Bst6I CTCTTC 1 cut(s) 248
BstBI TTCGAA 1 cut(s) 63
BstC8I GCNNGC 4 cut(s) 562, 890, 960, 1038
BstDEI CTNAG 4 cut(s) 657, 842, 963, 1053
BstDSI CCRYGG 1 cut(s) 348
BstF5I GGATG 2 cut(s) 8, 20
BstH2I RGCGCY 1 cut(s) 732
BstHHI GCGC 1 cut(s) 731
BstKTI GATC 6 cut(s) 87, 253, 267, 526, 870, 931
BstMBI GATC 6 cut(s) 84, 250, 264, 523, 867, 928
BstMWI GCNNNNNNNGC 2 cut(s) 117, 812
BstNI CCWGG 2 cut(s) 26, 599
BstNSI RCATGY 1 cut(s) 819
BstSCI CCNGG 5 cut(s) 24, 539, 597, 759, 760
BstV1I GCAGC 1 cut(s) 718
BstV2I GAAGAC 1 cut(s) 932
BstX2I RGATCY 1 cut(s) 928
BstXI CCANNNNNNTGG 1 cut(s) 291
BstYI RGATCY 1 cut(s) 928
Bsu36I CCTNAGG 1 cut(s) 657
BsuI GTATCC 1 cut(s) 167
BsuRI GGCC 6 cut(s) 80, 111, 152, 396, 544, 662
BtgI CCRYGG 1 cut(s) 348
BtsCI GGATG 2 cut(s) 8, 20
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 2 cut(s) 228, 774
Cac8I GCNNGC 4 cut(s) 562, 890, 960, 1038
CciI TCATGA 1 cut(s) 414
CfoI GCGC 1 cut(s) 731
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 5 cut(s) 79, 91, 110, 151, 498
Cfr9I CCCGGG 1 cut(s) 760
CseI GACGC 1 cut(s) 591
CsiI ACCWGGT 1 cut(s) 597
Csp6I GTAC 3 cut(s) 722, 776, 862
CviAII CATG 9 cut(s) 194, 349, 407, 415, 572, 677, 707, 725, 816
CviQI GTAC 3 cut(s) 722, 776, 862
DdeI CTNAG 4 cut(s) 657, 842, 963, 1053
DpnI GATC 6 cut(s) 86, 252, 266, 525, 869, 930
DpnII GATC 6 cut(s) 84, 250, 264, 523, 867, 928
EaeI YGGCCR 1 cut(s) 542
Eam1104I CTCTTC 1 cut(s) 248
EarI CTCTTC 1 cut(s) 248
EciI GGCGGA 1 cut(s) 746
Eco130I CCWWGG 1 cut(s) 348
Eco147I AGGCCT 1 cut(s) 396
Eco24I GRGCYC 1 cut(s) 474
Eco47I GGWCC 2 cut(s) 91, 498
Eco57I CTGAAG 1 cut(s) 926
Eco81I CCTNAGG 1 cut(s) 657
Eco88I CYCGRG 1 cut(s) 760
EcoO109I RGGNCCY 2 cut(s) 91, 498
EcoRII CCWGG 2 cut(s) 24, 597
EcoT14I CCWWGG 1 cut(s) 348
EcoT22I ATGCAT 1 cut(s) 817
EcoT38I GRGCYC 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 9 cut(s) 197, 352, 410, 418, 575, 680, 710, 728, 819
FalI AAGNNNNNCTT 2 cut(s) 694, 726
FaqI GGGAC 1 cut(s) 484
FatI CATG 9 cut(s) 193, 348, 406, 414, 571, 676, 706, 724, 815
FauI CCCGC 1 cut(s) 225
FbaI TGATCA 1 cut(s) 523
Fnu4HI GCNGC 3 cut(s) 165, 732, 807
FokI GGATG 1 cut(s) 7
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 3 cut(s) 165, 732, 807
FspBI CTAG 3 cut(s) 155, 392, 476
GlaI GCGC 1 cut(s) 730
GluI GCNGC 3 cut(s) 165, 732, 807
HaeII RGCGCY 1 cut(s) 732
HaeIII GGCC 6 cut(s) 80, 111, 152, 396, 544, 662
HapII CCGG 6 cut(s) 149, 168, 180, 541, 565, 761
HgaI GACGC 1 cut(s) 591
HhaI GCGC 1 cut(s) 731
Hin1II CATG 9 cut(s) 197, 352, 410, 418, 575, 680, 710, 728, 819
Hin6I GCGC 1 cut(s) 729
HinP1I GCGC 1 cut(s) 729
HincII GTYRAC 1 cut(s) 595
HindII GTYRAC 1 cut(s) 595
HinfI GANTC 4 cut(s) 5, 39, 568, 680
HpaI GTTAAC 1 cut(s) 595
HpaII CCGG 6 cut(s) 149, 168, 180, 541, 565, 761
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 2 cut(s) 595, 799
Hpy188I TCNGA 4 cut(s) 44, 523, 872, 945
Hpy188III TCNNGA 3 cut(s) 254, 262, 415
Hpy8I GTNNAC 2 cut(s) 595, 799
HpyAV CCTTC 4 cut(s) 390, 489, 829, 979
HpyCH4III ACNGT 1 cut(s) 126
HpyCH4IV ACGT 1 cut(s) 452
HpyCH4V TGCA 7 cut(s) 20, 446, 460, 575, 676, 734, 815
HpyF10VI GCNNNNNNNGC 2 cut(s) 117, 812
HpyF3I CTNAG 4 cut(s) 657, 842, 963, 1053
HpySE526I ACGT 1 cut(s) 452
Hsp92II CATG 9 cut(s) 197, 352, 410, 418, 575, 680, 710, 728, 819
HspAI GCGC 1 cut(s) 729
Ksp22I TGATCA 1 cut(s) 523
KspAI GTTAAC 1 cut(s) 595
Kzo9I GATC 6 cut(s) 84, 250, 264, 523, 867, 928
LmnI GCTCC 4 cut(s) 151, 286, 477, 988
Lsp1109I GCAGC 1 cut(s) 718
LweI GCATC 2 cut(s) 29, 349
MabI ACCWGGT 1 cut(s) 597
MaeI CTAG 3 cut(s) 155, 392, 476
MaeII ACGT 1 cut(s) 452
MaeIII GTNAC 1 cut(s) 690
MalI GATC 6 cut(s) 86, 252, 266, 525, 869, 930
MbiI CCGCTC 1 cut(s) 220
MboI GATC 6 cut(s) 84, 250, 264, 523, 867, 928
MboII GAAGA 3 cut(s) 77, 235, 932
MflI RGATCY 1 cut(s) 928
MhlI GDGCHC 1 cut(s) 474
MluCI AATT 4 cut(s) 59, 510, 697, 1058
MlyI GAGTC 1 cut(s) 689
MmeI TCCRAC 3 cut(s) 67, 160, 828
MnlI CCTC 8 cut(s) 63, 104, 450, 516, 529, 764, 837, 958
Mph1103I ATGCAT 1 cut(s) 817
MseI TTAA 3 cut(s) 594, 696, 1044
MslI CAYNNNNRTG 1 cut(s) 465
MspI CCGG 6 cut(s) 149, 168, 180, 541, 565, 761
MspR9I CCNGG 5 cut(s) 26, 541, 599, 761, 762
Mva1269I GAATGC 1 cut(s) 817
MvaI CCWGG 2 cut(s) 26, 599
MwoI GCNNNNNNNGC 2 cut(s) 117, 812
NciI CCSGG 3 cut(s) 541, 761, 762
NcoI CCATGG 1 cut(s) 348
NdeII GATC 6 cut(s) 84, 250, 264, 523, 867, 928
NlaIII CATG 9 cut(s) 197, 352, 410, 418, 575, 680, 710, 728, 819
NlaIV GGNNCC 3 cut(s) 473, 500, 786
NsiI ATGCAT 1 cut(s) 817
NspI RCATGY 1 cut(s) 819
NspV TTCGAA 1 cut(s) 63
PagI TCATGA 1 cut(s) 414
PceI AGGCCT 1 cut(s) 396
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PctI GAATGC 1 cut(s) 817
PfeI GAWTC 2 cut(s) 39, 568
PkrI GCNGC 3 cut(s) 166, 733, 808
PleI GAGTC 1 cut(s) 688
PpsI GAGTC 1 cut(s) 688
PpuMI RGGWCCY 2 cut(s) 91, 498
PsiI TTATAA 1 cut(s) 300
Psp1406I AACGTT 1 cut(s) 452
Psp5II RGGWCCY 2 cut(s) 91, 498
Psp6I CCWGG 2 cut(s) 24, 597
PspGI CCWGG 2 cut(s) 24, 597
PspN4I GGNNCC 3 cut(s) 473, 500, 786
PspPI GGNCC 5 cut(s) 79, 91, 110, 151, 498
PspPPI RGGWCCY 2 cut(s) 91, 498
PsuI RGATCY 1 cut(s) 928
RsaI GTAC 3 cut(s) 723, 777, 863
RsaNI GTAC 3 cut(s) 722, 776, 862
RseI CAYNNNNRTG 1 cut(s) 465
SaqAI TTAA 3 cut(s) 594, 696, 1044
SatI GCNGC 3 cut(s) 165, 732, 807
Sau3AI GATC 6 cut(s) 84, 250, 264, 523, 867, 928
Sau96I GGNCC 5 cut(s) 79, 91, 110, 151, 498
SchI GAGTC 1 cut(s) 689
ScrFI CCNGG 5 cut(s) 26, 541, 599, 761, 762
SduI GDGCHC 1 cut(s) 474
SexAI ACCWGGT 1 cut(s) 597
SfaNI GCATC 2 cut(s) 29, 349
SfuI TTCGAA 1 cut(s) 63
SgrAI CRCCGGYG 1 cut(s) 167
SinI GGWCC 2 cut(s) 91, 498
SmaI CCCGGG 1 cut(s) 762
SmiMI CAYNNNNRTG 1 cut(s) 465
Sse9I AATT 4 cut(s) 59, 510, 697, 1058
SseBI AGGCCT 1 cut(s) 396
SsiI CCGC 4 cut(s) 165, 218, 757, 806
SspMI CTAG 3 cut(s) 155, 392, 476
StuI AGGCCT 1 cut(s) 396
StyD4I CCNGG 5 cut(s) 24, 539, 597, 759, 760
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 1 cut(s) 126
TaiI ACGT 1 cut(s) 455
TaqI TCGA 2 cut(s) 55, 63
TasI AATT 4 cut(s) 59, 510, 697, 1058
TatI WGTACW 1 cut(s) 861
TauI GCSGC 2 cut(s) 167, 809
TfiI GAWTC 2 cut(s) 39, 568
Tru1I TTAA 3 cut(s) 594, 696, 1044
Tru9I TTAA 3 cut(s) 594, 696, 1044
TscAI CASTG 2 cut(s) 235, 774
TseI GCWGC 1 cut(s) 731
TspDTI ATGAA 1 cut(s) 403
TspMI CCCGGG 1 cut(s) 760
TspRI CASTG 2 cut(s) 235, 774
VpaK11BI GGWCC 2 cut(s) 91, 498
XapI RAATTY 3 cut(s) 59, 510, 1058
XceI RCATGY 1 cut(s) 819
XcmI CCANNNNNNNNNTGG 1 cut(s) 713
XmaI CCCGGG 1 cut(s) 760
XspI CTAG 3 cut(s) 155, 392, 476
Zsp2I ATGCAT 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.