Rh4DG370900

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001444
Physical Location & Seq
Forward (+)
58976137 .. 58977216
1080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG370900.1

Sequence Viewer

Length: 1080 bp
ATGAACTCTACCGAAACTCCATTGGTAGAAAGAATCAGACCCTATCCTCGAAAATTCGAAGACCTCATAATGGCTGATATCAAGAACTTCACACTCACTGTCGGACCACGAATCCCAATGTGTAGAGTTCAACACAATGCTCCTGCGCTAGTATTCACCGCCGGTGGATACACCGGAAACTTTTGGCATGACTTCAACGATGGATTCATCCCTCTCTTTATCACCGCCAACACCATATTCCCAGATCAAGACTTCGTCATTGTGGTTTCCGAAGCTCCCAAGTGGTGGCCTAGTAAGTATGCCGATTTACTACGCATGTTCACCAATCATCCTATTGTCAACCTAGGAAGTGATACCACCACCCATTGTTTTCCTTCTGCCAAGCTAGGCCTTATATCACATGGCTTCATGATCATAAACCAAACATTAATACCCAACTCAAAGACATACATGAATTTTCGTGAGCTCCTTGGCAAAGCCTATAACCAACTAGCCCAAATCCAAACCAAAATTTTCACCTCTAAGCCCACAAATCCTCGGCCCAGACTTGTTTTGGCGAGCCGACGTCAGGCCACTGGACGCACGATCATGAACCAAGCCCAAGTTATTCGTTTGATCAAGAAAGTAGGTTTTGATGTGGTGGTATTTGAGCCTAAAACCAAAACCTCATTGCAAGAATCTTATGCATTACTGAATTCAAGCCATGCTTTTGTTGGGGTTCATGGAGCTGCACTTACGCACTCATTGTTCCTCCGGCCGGGCGCGGTGTTCGTGCAGGTGGTGCCAATAGGAATAGGCTGGGCGGCGGAAGCGTTTTTCGGGAGAGTAGCAAAAGGGTTGAATTTGGAGTATATTGAGTACAAAATTGGTGTGGAAGAGAGTAGCTTGGTGGACAGGTTTGGAAAGGACAGTTTGTTGGTTAGGGATCCATTTGCTCTTCAAAAAAGGGGCTGGCCCCCGGAGGTTATGAACACTTACTTGAAAGAACAGAATGTGAAACTGGATTTGGTTAGGTTCAAGAGATATTTGAAGAAAGCTTTTAAGAAAGCTAAGAGATTTATGGACAATGAGGGTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

359

Amino Acids

40.79

Weight (kDa)

9.81

Isoelectric Point (pI)

24.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 62 - 258 9.4e-16 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 766
AatII GACGTC 1 cut(s) 568
Acc36I ACCTGC 1 cut(s) 766
AccB1I GGYRCC 1 cut(s) 781
AccB7I CCANNNNNTGG 1 cut(s) 285
AccII CGCG 1 cut(s) 764
AciI CCGC 5 cut(s) 159, 225, 764, 803, 806
AclWI GGATC 2 cut(s) 920, 933
AcoI YGGCCR 1 cut(s) 755
AcsI RAATTY 5 cut(s) 53, 454, 510, 694, 841
AcyI GRCGYC 1 cut(s) 565
AfaI GTAC 1 cut(s) 860
AfiI CCNNNNNNNGG 4 cut(s) 70, 285, 568, 757
AgsI TTSAA 8 cut(s) 131, 196, 699, 841, 941, 982, 1018, 1030
AjuI GAANNNNNNNTTGG 4 cut(s) 221, 253, 989, 1021
AluBI AGCT 7 cut(s) 275, 385, 466, 728, 885, 1037, 1049
AluI AGCT 7 cut(s) 275, 385, 466, 728, 885, 1037, 1049
Alw21I GWGCWC 1 cut(s) 468
AlwI GGATC 2 cut(s) 920, 933
AoxI GGCC 6 cut(s) 287, 388, 539, 570, 755, 953
ApeKI GCWGC 1 cut(s) 728
ApoI RAATTY 5 cut(s) 53, 454, 510, 694, 841
AseI ATTAAT 1 cut(s) 428
AspA2I CCTAGG 1 cut(s) 343
AspLEI GCGC 2 cut(s) 148, 764
AspS9I GGNCC 3 cut(s) 104, 540, 954
AsuC2I CCSGG 2 cut(s) 759, 959
AsuHPI GGTGA 4 cut(s) 148, 214, 313, 508
AsuII TTCGAA 1 cut(s) 57
AvaII GGWCC 1 cut(s) 104
AvrII CCTAGG 1 cut(s) 343
BamHI GGATCC 1 cut(s) 925
BanI GGYRCC 1 cut(s) 781
BanII GRGCYC 1 cut(s) 468
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 468
BbvI GCAGC 1 cut(s) 715
BccI CCATC 1 cut(s) 194
BciVI GTATCC 1 cut(s) 161
BclI TGATCA 2 cut(s) 411, 615
BcnI CCSGG 2 cut(s) 759, 959
BfaI CTAG 5 cut(s) 149, 291, 344, 386, 491
BfuAI ACCTGC 1 cut(s) 766
BfuI GTATCC 1 cut(s) 161
BisI GCNGC 2 cut(s) 729, 804
BlnI CCTAGG 1 cut(s) 343
BlsI GCNGC 2 cut(s) 730, 805
Bme1390I CCNGG 2 cut(s) 759, 959
Bme18I GGWCC 1 cut(s) 104
BmgT120I GGNCC 3 cut(s) 104, 540, 954
BmiI GGNNCC 3 cut(s) 783, 927, 956
BmrFI CCNGG 2 cut(s) 759, 959
BpiI GAAGAC 1 cut(s) 66
Bpu14I TTCGAA 1 cut(s) 57
BpuMI CCSGG 2 cut(s) 759, 959
BsaHI GRCGYC 1 cut(s) 565
BsaJI CCNNGG 4 cut(s) 343, 469, 536, 957
BsaWI WCCGGW 1 cut(s) 173
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 4 cut(s) 70, 285, 568, 757
Bse118I RCCGGY 1 cut(s) 161
Bse1I ACTGG 2 cut(s) 580, 1005
Bse3DI GCAATG 1 cut(s) 668
BseDI CCNNGG 4 cut(s) 343, 469, 536, 957
BseGI GGATG 2 cut(s) 207, 328
BseLI CCNNNNNNNGG 4 cut(s) 70, 285, 568, 757
BseMI GCAATG 1 cut(s) 668
BseNI ACTGG 2 cut(s) 580, 1005
BseX3I CGGCCG 1 cut(s) 755
BseXI GCAGC 1 cut(s) 715
BseYI CCCAGC 1 cut(s) 798
BsgI GTGCAG 2 cut(s) 714, 794
Bsh1236I CGCG 1 cut(s) 764
Bsh1285I CGRYCG 1 cut(s) 758
BshFI GGCC 6 cut(s) 289, 390, 541, 572, 757, 955
BshNI GGYRCC 1 cut(s) 781
BsiEI CGRYCG 1 cut(s) 758
BsiHKAI GWGCWC 1 cut(s) 468
BsiSI CCGG 5 cut(s) 162, 174, 754, 758, 959
BslI CCNNNNNNNGG 4 cut(s) 70, 285, 568, 757
BsnI GGCC 6 cut(s) 289, 390, 541, 572, 757, 955
Bsp119I TTCGAA 1 cut(s) 57
Bsp1286I GDGCHC 1 cut(s) 468
Bsp143I GATC 5 cut(s) 244, 411, 585, 615, 925
BspACI CCGC 5 cut(s) 159, 225, 764, 803, 806
BspANI GGCC 6 cut(s) 289, 390, 541, 572, 757, 955
BspFNI CGCG 1 cut(s) 764
BspHI TCATGA 2 cut(s) 408, 588
BspLI GGNNCC 3 cut(s) 783, 927, 956
BspMI ACCTGC 1 cut(s) 766
BspPI GGATC 2 cut(s) 920, 933
BspQI GCTCTTC 1 cut(s) 942
BspT104I TTCGAA 1 cut(s) 57
BspT107I GGYRCC 1 cut(s) 781
BsrDI GCAATG 1 cut(s) 668
BsrFI RCCGGY 1 cut(s) 161
BsrI ACTGG 2 cut(s) 580, 1005
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 4 cut(s) 343, 469, 536, 957
BssMI GATC 5 cut(s) 244, 411, 585, 615, 925
BssNI GRCGYC 1 cut(s) 565
BssT1I CCWWGG 2 cut(s) 343, 469
Bst4CI ACNGT 2 cut(s) 100, 911
Bst6I CTCTTC 2 cut(s) 870, 942
BstACI GRCGYC 1 cut(s) 565
BstAPI GCANNNNNTGC 1 cut(s) 781
BstBI TTCGAA 1 cut(s) 57
BstC8I GCNNGC 2 cut(s) 559, 953
BstDEI CTNAG 2 cut(s) 522, 1050
BstF5I GGATG 2 cut(s) 207, 328
BstFNI CGCG 1 cut(s) 764
BstHHI GCGC 2 cut(s) 148, 764
BstKTI GATC 5 cut(s) 247, 414, 588, 618, 928
BstMBI GATC 5 cut(s) 244, 411, 585, 615, 925
BstMCI CGRYCG 1 cut(s) 758
BstMWI GCNNNNNNNGC 2 cut(s) 781, 809
BstNSI RCATGY 1 cut(s) 319
BstSCI CCNGG 2 cut(s) 757, 957
BstUI CGCG 1 cut(s) 764
BstV1I GCAGC 1 cut(s) 715
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 1 cut(s) 925
BstYI RGATCY 1 cut(s) 925
BstZI CGGCCG 1 cut(s) 755
BsuI GTATCC 1 cut(s) 161
BsuRI GGCC 6 cut(s) 289, 390, 541, 572, 757, 955
BtsCI GGATG 2 cut(s) 207, 328
BtsIMutI CAGTG 2 cut(s) 96, 573
BveI ACCTGC 1 cut(s) 766
Cac8I GCNNGC 2 cut(s) 559, 953
CciI TCATGA 2 cut(s) 408, 588
CfoI GCGC 2 cut(s) 148, 764
Cfr10I RCCGGY 1 cut(s) 161
Cfr13I GGNCC 3 cut(s) 104, 540, 954
CseI GACGC 1 cut(s) 588
Csp6I GTAC 1 cut(s) 859
CviAII CATG 8 cut(s) 188, 316, 401, 409, 451, 589, 704, 722
CviQI GTAC 1 cut(s) 859
DdeI CTNAG 2 cut(s) 522, 1050
DpnI GATC 5 cut(s) 246, 413, 587, 617, 927
DpnII GATC 5 cut(s) 244, 411, 585, 615, 925
EaeI YGGCCR 1 cut(s) 755
EagI CGGCCG 1 cut(s) 755
Eam1104I CTCTTC 2 cut(s) 870, 942
EarI CTCTTC 2 cut(s) 870, 942
EciI GGCGGA 1 cut(s) 821
Ecl136II GAGCTC 1 cut(s) 466
EclXI CGGCCG 1 cut(s) 755
Eco130I CCWWGG 2 cut(s) 343, 469
Eco147I AGGCCT 1 cut(s) 390
Eco24I GRGCYC 1 cut(s) 468
Eco32I GATATC 1 cut(s) 79
Eco47I GGWCC 1 cut(s) 104
Eco52I CGGCCG 1 cut(s) 755
Eco53kI GAGCTC 1 cut(s) 466
EcoICRI GAGCTC 1 cut(s) 466
EcoRI GAATTC 1 cut(s) 694
EcoRV GATATC 1 cut(s) 79
EcoT14I CCWWGG 2 cut(s) 343, 469
EcoT22I ATGCAT 1 cut(s) 688
EcoT38I GRGCYC 1 cut(s) 468
ErhI CCWWGG 2 cut(s) 343, 469
FaeI CATG 8 cut(s) 191, 319, 404, 412, 454, 592, 707, 725
FalI AAGNNNNNCTT 2 cut(s) 691, 723
FatI CATG 8 cut(s) 187, 315, 400, 408, 450, 588, 703, 721
FbaI TGATCA 2 cut(s) 411, 615
Fnu4HI GCNGC 2 cut(s) 729, 804
FokI GGATG 2 cut(s) 194, 315
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 2 cut(s) 729, 804
FspBI CTAG 5 cut(s) 149, 291, 344, 386, 491
GlaI GCGC 2 cut(s) 147, 763
GluI GCNGC 2 cut(s) 729, 804
GsaI CCCAGC 1 cut(s) 802
HaeIII GGCC 6 cut(s) 289, 390, 541, 572, 757, 955
HapII CCGG 5 cut(s) 162, 174, 754, 758, 959
HgaI GACGC 1 cut(s) 588
HhaI GCGC 2 cut(s) 148, 764
Hin1I GRCGYC 1 cut(s) 565
Hin1II CATG 8 cut(s) 191, 319, 404, 412, 454, 592, 707, 725
Hin6I GCGC 2 cut(s) 146, 762
HinP1I GCGC 2 cut(s) 146, 762
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 1 cut(s) 1035
HinfI GANTC 4 cut(s) 33, 111, 204, 677
HpaII CCGG 5 cut(s) 162, 174, 754, 758, 959
HphI GGTGA 4 cut(s) 148, 214, 313, 508
Hpy166II GTNNAC 3 cut(s) 321, 340, 892
Hpy188I TCNGA 3 cut(s) 38, 104, 271
Hpy188III TCNNGA 8 cut(s) 82, 248, 409, 461, 589, 619, 820, 1018
Hpy8I GTNNAC 3 cut(s) 321, 340, 892
Hpy99I CGWCG 1 cut(s) 567
HpyAV CCTTC 1 cut(s) 384
HpyCH4III ACNGT 2 cut(s) 100, 911
HpyCH4IV ACGT 1 cut(s) 565
HpyCH4V TGCA 4 cut(s) 673, 686, 731, 775
HpyF10VI GCNNNNNNNGC 2 cut(s) 781, 809
HpyF3I CTNAG 2 cut(s) 522, 1050
HpySE526I ACGT 1 cut(s) 565
Hsp92I GRCGYC 1 cut(s) 565
Hsp92II CATG 8 cut(s) 191, 319, 404, 412, 454, 592, 707, 725
HspAI GCGC 2 cut(s) 146, 762
Ksp22I TGATCA 2 cut(s) 411, 615
Kzo9I GATC 5 cut(s) 244, 411, 585, 615, 925
LguI GCTCTTC 1 cut(s) 942
LmnI GCTCC 4 cut(s) 145, 280, 471, 725
Lsp1109I GCAGC 1 cut(s) 715
MaeI CTAG 5 cut(s) 149, 291, 344, 386, 491
MaeII ACGT 1 cut(s) 565
MalI GATC 5 cut(s) 246, 413, 587, 617, 927
MboI GATC 5 cut(s) 244, 411, 585, 615, 925
MboII GAAGA 4 cut(s) 71, 887, 929, 1042
MflI RGATCY 1 cut(s) 925
MhlI GDGCHC 1 cut(s) 468
MluCI AATT 6 cut(s) 53, 454, 510, 694, 841, 864
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 9 cut(s) 57, 74, 222, 529, 546, 676, 761, 955, 1063
Mph1103I ATGCAT 1 cut(s) 688
MseI TTAA 2 cut(s) 428, 1041
MslI CAYNNNNRTG 1 cut(s) 587
MspI CCGG 5 cut(s) 162, 174, 754, 758, 959
MspR9I CCNGG 2 cut(s) 759, 959
MvnI CGCG 1 cut(s) 764
MwoI GCNNNNNNNGC 2 cut(s) 781, 809
NciI CCSGG 2 cut(s) 759, 959
NdeII GATC 5 cut(s) 244, 411, 585, 615, 925
NlaIII CATG 8 cut(s) 191, 319, 404, 412, 454, 592, 707, 725
NlaIV GGNNCC 3 cut(s) 783, 927, 956
NmeAIII GCCGAG 1 cut(s) 517
NsiI ATGCAT 1 cut(s) 688
NspI RCATGY 1 cut(s) 319
NspV TTCGAA 1 cut(s) 57
PagI TCATGA 2 cut(s) 408, 588
PaqCI CACCTGC 1 cut(s) 766
PceI AGGCCT 1 cut(s) 390
PciSI GCTCTTC 1 cut(s) 942
PfeI GAWTC 4 cut(s) 33, 111, 204, 677
PflFI GACNNNGTC 1 cut(s) 254
PflMI CCANNNNNTGG 1 cut(s) 285
PkrI GCNGC 2 cut(s) 730, 805
PshBI ATTAAT 1 cut(s) 428
Psp124BI GAGCTC 1 cut(s) 468
PspFI CCCAGC 1 cut(s) 798
PspN4I GGNNCC 3 cut(s) 783, 927, 956
PspPI GGNCC 3 cut(s) 104, 540, 954
PsuI RGATCY 1 cut(s) 925
PsyI GACNNNGTC 1 cut(s) 254
RsaI GTAC 1 cut(s) 860
RsaNI GTAC 1 cut(s) 859
RseI CAYNNNNRTG 1 cut(s) 587
SacI GAGCTC 1 cut(s) 468
SapI GCTCTTC 1 cut(s) 942
SaqAI TTAA 2 cut(s) 428, 1041
SatI GCNGC 2 cut(s) 729, 804
Sau3AI GATC 5 cut(s) 244, 411, 585, 615, 925
Sau96I GGNCC 3 cut(s) 104, 540, 954
ScrFI CCNGG 2 cut(s) 759, 959
SduI GDGCHC 1 cut(s) 468
SfuI TTCGAA 1 cut(s) 57
SgrAI CRCCGGYG 1 cut(s) 161
SinI GGWCC 1 cut(s) 104
SmiMI CAYNNNNRTG 1 cut(s) 587
Sse9I AATT 6 cut(s) 53, 454, 510, 694, 841, 864
SseBI AGGCCT 1 cut(s) 390
SsiI CCGC 5 cut(s) 159, 225, 764, 803, 806
SspMI CTAG 5 cut(s) 149, 291, 344, 386, 491
SstI GAGCTC 1 cut(s) 468
StuI AGGCCT 1 cut(s) 390
StyD4I CCNGG 2 cut(s) 757, 957
StyI CCWWGG 2 cut(s) 343, 469
TaaI ACNGT 2 cut(s) 100, 911
TaiI ACGT 1 cut(s) 568
TaqI TCGA 2 cut(s) 49, 57
TasI AATT 6 cut(s) 53, 454, 510, 694, 841, 864
TatI WGTACW 1 cut(s) 858
TauI GCSGC 1 cut(s) 806
TfiI GAWTC 4 cut(s) 33, 111, 204, 677
Tru1I TTAA 2 cut(s) 428, 1041
Tru9I TTAA 2 cut(s) 428, 1041
TscAI CASTG 2 cut(s) 103, 580
TseI GCWGC 1 cut(s) 728
TspDTI ATGAA 7 cut(s) 17, 196, 397, 467, 605, 710, 983
TspRI CASTG 2 cut(s) 103, 580
Tth111I GACNNNGTC 1 cut(s) 254
Van91I CCANNNNNTGG 1 cut(s) 285
VpaK11BI GGWCC 1 cut(s) 104
VspI ATTAAT 1 cut(s) 428
XapI RAATTY 5 cut(s) 53, 454, 510, 694, 841
XceI RCATGY 1 cut(s) 319
XcmI CCANNNNNNNNNTGG 2 cut(s) 550, 710
XmaJI CCTAGG 1 cut(s) 343
XspI CTAG 5 cut(s) 149, 291, 344, 386, 491
ZraI GACGTC 1 cut(s) 566
Zsp2I ATGCAT 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.