Rroxscaffold_5G00379930

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001444
Physical Location & Seq
Forward (+)
60137818 .. 60140364
2547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00379930.1

Sequence Viewer

Length: 1386 bp
ATGAACAAGAAAGAGTCAATAACAGCGATTTTAGGCGTGTCGCTCTTGGCGGTGGTGTTTGTTTGCCACATTAATCTCTCAGCAAATTTGAGGTTCAACTTCGAACTCATTCACTCACTTGCAACTACTCCAAAACCAGATATCTTCAAACGCTTAGCACCGAGATCAAATAATAGCGGCAGGAATCCTATCAATTCGACACGTTTGCCAGCTTCGACATCAACAGAGGTTAGATGCACCCGTTCTCACAAATACTACGATTTCTGTTTCATCGACGGCCCAACAGTTCTGGACGCCACAACCTCCACGTTATTTGTAATGGACCCCACAGGCCCAACCTTGGTAGAAAAAATCAAGCCCTACCCACGAAAACATGAGAATTCGACAATGTCGGGAATCAAAGAGCTCACCCTCACATCAGGCCCACCAGGCCCAAAATGCCAAGTCCAACACAATGCCCCGGCAATTGTATTCAGTGCAGGAGGGTACACCGGAAACTTCTTCCATGATTTCAATGATGGGTTCATCCCCCTCTACATCATAGTCAATTCGATTCTCTACGATCAAGATCCCGTGCTAGTGATTTCCAAAGCACGTGATTGGTGGCTTCAGAGGTACTCAGATTTGCTACAGGTTTTTAGCGAGCACCCAATTATAAACCTTGATAATGATACTGCCACCCATTGTTTTCGTTCTGCCACCGTGGGTCTAATTTCTCATGGATTTTTGACCATAAGCCCCAAATTGTTAAAGACCCCCAAGACACTGCCTCATTTCCATGCCATCCTAGGCAAAGCCTTTGGCCAAACTGCCCAACGCTTCTACCGTCAGCGCCACCACAATGAGCGGCCACGCATAGTGTTAATTAGTCGAACCGTAGGCGTCGGCCGAGCAATCTTGAATCAAGAGGAAGCGATGGAAGTGGCAGACAGAATAGGGTTTGAGGTGATTGTTTATGAGCCAAGTTCAAGGACCGACTTGCATGTAGCATATGACTTGCTCAGTAATAGCCATGCAGTGGTTGGTGTTCATGGTGCGGCCCTTACTCACTTGTTGTTCATGCGGCCGGGGACGGTGTTCATGCAAATTGTGCCGCTAGGGACTAATTGGTTGGCAAAAGTGTGCTATGAAAATCCAGGTAGGGCAATGGGGCTGGAGTACATTGACTACAGGATTACGGCGGAGGAGAGCAGCTTGGTAGAAACTTACGGAAAGGATGACATTGTGGTTAAGAATCCGGCAGCTAAGATTGGTCCTAATTGGTCCATGGAGATTATGAAGATTTATCTCAAGGAACAGAATGTCAGGTTGGATTTGGATAGGTTTTCATGGTTTTTGAAGGCCGCTTATGACAAGGCCAAGAAGTTTATGGACAAGGAAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

461

Amino Acids

51.84

Weight (kDa)

8.93

Isoelectric Point (pI)

27.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 184 - 360 4.4e-14 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 656
AccB7I CCANNNNNTGG 1 cut(s) 1018
AccBSI CCGCTC 1 cut(s) 847
AciI CCGC 8 cut(s) 50, 177, 847, 1037, 1063, 1094, 1181, 1344
AclWI GGATC 1 cut(s) 563
AcoI YGGCCR 4 cut(s) 802, 848, 886, 1064
AcsI RAATTY 2 cut(s) 85, 379
AcuI CTGAAG 1 cut(s) 593
AcvI CACGTG 1 cut(s) 596
AcyI GRCGYC 2 cut(s) 294, 882
AfaI GTAC 3 cut(s) 488, 617, 1160
AfiI CCNNNNNNNGG 2 cut(s) 340, 1018
AflIII ACRYGT 1 cut(s) 200
AgsI TTSAA 6 cut(s) 97, 148, 514, 901, 969, 1339
AjnI CCWGG 2 cut(s) 427, 1135
AjuI GAANNNNNNNTTGG 2 cut(s) 1292, 1324
AluBI AGCT 4 cut(s) 212, 406, 1194, 1244
AluI AGCT 4 cut(s) 212, 406, 1194, 1244
Alw21I GWGCWC 2 cut(s) 408, 648
AlwI GGATC 1 cut(s) 563
ApeKI GCWGC 2 cut(s) 1191, 1241
ApoI RAATTY 2 cut(s) 85, 379
AseI ATTAAT 1 cut(s) 72
Asp700I GAANNNNTTC 1 cut(s) 108
AspA2I CCTAGG 1 cut(s) 787
AspLEI GCGC 1 cut(s) 834
AspS9I GGNCC 9 cut(s) 278, 322, 332, 422, 431, 972, 1039, 1253, 1263
AsuC2I CCSGG 2 cut(s) 461, 1068
AsuHPI GGTGA 2 cut(s) 400, 958
AsuII TTCGAA 1 cut(s) 102
AvaII GGWCC 4 cut(s) 322, 972, 1253, 1263
AvrII CCTAGG 1 cut(s) 787
BalI TGGCCA 1 cut(s) 804
BanII GRGCYC 1 cut(s) 408
BbrPI CACGTG 1 cut(s) 596
Bbv12I GWGCWC 2 cut(s) 408, 648
BbvI GCAGC 2 cut(s) 1203, 1253
BccI CCATC 3 cut(s) 512, 791, 910
BceAI ACGGC 2 cut(s) 292, 1194
BcgI CGANNNNNNTGC 2 cut(s) 187, 221
BciT130I CCWGG 2 cut(s) 429, 1137
BcnI CCSGG 2 cut(s) 461, 1068
BfaI CTAG 3 cut(s) 578, 788, 1097
BfmI CTRYAG 2 cut(s) 629, 1168
BfoI RGCGCY 1 cut(s) 835
BglI GCCNNNNNGGC 1 cut(s) 429
BisI GCNGC 8 cut(s) 178, 848, 1038, 1064, 1094, 1192, 1242, 1344
BlnI CCTAGG 1 cut(s) 787
BlpI GCTNAGC 1 cut(s) 154
BlsI GCNGC 8 cut(s) 179, 849, 1039, 1065, 1095, 1193, 1243, 1345
Bme1390I CCNGG 4 cut(s) 429, 461, 1068, 1137
Bme18I GGWCC 4 cut(s) 322, 972, 1253, 1263
BmgT120I GGNCC 9 cut(s) 278, 322, 332, 422, 431, 972, 1039, 1253, 1263
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 4 cut(s) 429, 461, 1068, 1137
BmsI GCATC 1 cut(s) 224
BpmI CTGGAG 1 cut(s) 1175
Bpu1102I GCTNAGC 1 cut(s) 154
Bpu14I TTCGAA 1 cut(s) 102
BpuEI CTTGAG 1 cut(s) 1274
BpuMI CCSGG 2 cut(s) 461, 1068
BsaAI YACGTR 1 cut(s) 596
BsaBI GATNNNNATC 1 cut(s) 567
BsaHI GRCGYC 2 cut(s) 294, 882
BsaJI CCNNGG 6 cut(s) 339, 459, 702, 787, 1067, 1266
BsaWI WCCGGW 1 cut(s) 491
Bsc4I CCNNNNNNNGG 2 cut(s) 340, 1018
Bse3DI GCAATG 1 cut(s) 1152
Bse8I GATNNNNATC 1 cut(s) 567
BseBI CCWGG 2 cut(s) 429, 1137
BseDI CCNNGG 6 cut(s) 339, 459, 702, 787, 1067, 1266
BseGI GGATG 3 cut(s) 525, 783, 1222
BseJI GATNNNNATC 1 cut(s) 567
BseLI CCNNNNNNNGG 2 cut(s) 340, 1018
BseMI GCAATG 1 cut(s) 1152
BseMII CTCAG 3 cut(s) 93, 633, 1015
BseRI GAGGAG 1 cut(s) 1199
BseX3I CGGCCG 2 cut(s) 886, 1064
BseXI GCAGC 2 cut(s) 1203, 1253
BsgI GTGCAG 1 cut(s) 498
Bsh1285I CGRYCG 2 cut(s) 889, 1067
BsiEI CGRYCG 2 cut(s) 889, 1067
BsiHKAI GWGCWC 2 cut(s) 408, 648
BsiSI CCGG 4 cut(s) 461, 492, 1067, 1238
BslFI GGGAC 2 cut(s) 1084, 1114
BslI CCNNNNNNNGG 2 cut(s) 340, 1018
BsmFI GGGAC 2 cut(s) 1084, 1114
Bsp119I TTCGAA 1 cut(s) 102
Bsp1286I GDGCHC 2 cut(s) 408, 648
Bsp143I GATC 3 cut(s) 164, 562, 568
Bsp1720I GCTNAGC 1 cut(s) 154
Bsp19I CCATGG 1 cut(s) 1266
BspACI CCGC 8 cut(s) 50, 177, 847, 1037, 1063, 1094, 1181, 1344
BspCNI CTCAG 3 cut(s) 92, 632, 1014
BspLI GGNNCC 1 cut(s) 324
BspPI GGATC 1 cut(s) 563
BspT104I TTCGAA 1 cut(s) 102
BsrBI CCGCTC 1 cut(s) 847
BsrDI GCAATG 1 cut(s) 1152
BssECI CCNNGG 6 cut(s) 339, 459, 702, 787, 1067, 1266
BssMI GATC 3 cut(s) 164, 562, 568
BssNI GRCGYC 2 cut(s) 294, 882
BssT1I CCWWGG 3 cut(s) 339, 787, 1266
Bst2UI CCWGG 2 cut(s) 429, 1137
Bst4CI ACNGT 5 cut(s) 286, 703, 827, 877, 1075
BstACI GRCGYC 2 cut(s) 294, 882
BstAPI GCANNNNNTGC 1 cut(s) 1090
BstBAI YACGTR 1 cut(s) 596
BstBI TTCGAA 1 cut(s) 102
BstC8I GCNNGC 2 cut(s) 210, 644
BstDEI CTNAG 5 cut(s) 79, 154, 619, 1001, 1245
BstDSI CCRYGG 2 cut(s) 702, 1266
BstF5I GGATG 3 cut(s) 525, 783, 1222
BstH2I RGCGCY 1 cut(s) 835
BstHHI GCGC 1 cut(s) 834
BstKTI GATC 3 cut(s) 167, 565, 571
BstMBI GATC 3 cut(s) 164, 562, 568
BstMCI CGRYCG 2 cut(s) 889, 1067
BstMWI GCNNNNNNNGC 3 cut(s) 429, 438, 1090
BstNI CCWGG 2 cut(s) 429, 1137
BstNSI RCATGY 1 cut(s) 986
BstSCI CCNGG 4 cut(s) 427, 459, 1066, 1135
BstSFI CTRYAG 2 cut(s) 629, 1168
BstV1I GCAGC 2 cut(s) 1203, 1253
BstX2I RGATCY 1 cut(s) 568
BstYI RGATCY 1 cut(s) 568
BstZI CGGCCG 2 cut(s) 886, 1064
BtgI CCRYGG 2 cut(s) 702, 1266
BtgZI GCGATG 1 cut(s) 929
BtsCI GGATG 3 cut(s) 525, 783, 1222
BtsI GCAGTG 2 cut(s) 764, 1023
BtsIMutI CAGTG 3 cut(s) 481, 764, 1023
Cac8I GCNNGC 2 cut(s) 210, 644
CfoI GCGC 1 cut(s) 834
Cfr13I GGNCC 9 cut(s) 278, 322, 332, 422, 431, 972, 1039, 1253, 1263
CseI GACGC 2 cut(s) 302, 871
Csp6I GTAC 3 cut(s) 487, 616, 1159
CspCI CAANNNNNGTGG 2 cut(s) 295, 330
CviQI GTAC 3 cut(s) 487, 616, 1159
DdeI CTNAG 5 cut(s) 79, 154, 619, 1001, 1245
DpnI GATC 3 cut(s) 166, 564, 570
DpnII GATC 3 cut(s) 164, 562, 568
EaeI YGGCCR 4 cut(s) 802, 848, 886, 1064
EagI CGGCCG 2 cut(s) 886, 1064
EciI GGCGGA 1 cut(s) 1196
Ecl136II GAGCTC 1 cut(s) 406
EclXI CGGCCG 2 cut(s) 886, 1064
Eco130I CCWWGG 3 cut(s) 339, 787, 1266
Eco24I GRGCYC 1 cut(s) 408
Eco32I GATATC 1 cut(s) 142
Eco47I GGWCC 4 cut(s) 322, 972, 1253, 1263
Eco52I CGGCCG 2 cut(s) 886, 1064
Eco53kI GAGCTC 1 cut(s) 406
Eco57I CTGAAG 1 cut(s) 593
Eco72I CACGTG 1 cut(s) 596
EcoICRI GAGCTC 1 cut(s) 406
EcoRI GAATTC 1 cut(s) 379
EcoRII CCWGG 2 cut(s) 427, 1135
EcoRV GATATC 1 cut(s) 142
EcoT14I CCWWGG 3 cut(s) 339, 787, 1266
EcoT38I GRGCYC 1 cut(s) 408
ErhI CCWWGG 3 cut(s) 339, 787, 1266
FaqI GGGAC 2 cut(s) 1084, 1114
FauNDI CATATG 1 cut(s) 991
Fnu4HI GCNGC 8 cut(s) 178, 848, 1038, 1064, 1094, 1192, 1242, 1344
FokI GGATG 3 cut(s) 512, 770, 1229
FriOI GRGCYC 1 cut(s) 408
Fsp4HI GCNGC 8 cut(s) 178, 848, 1038, 1064, 1094, 1192, 1242, 1344
FspBI CTAG 3 cut(s) 578, 788, 1097
GlaI GCGC 1 cut(s) 833
GluI GCNGC 8 cut(s) 178, 848, 1038, 1064, 1094, 1192, 1242, 1344
GsuI CTGGAG 1 cut(s) 1175
HaeII RGCGCY 1 cut(s) 835
HapII CCGG 4 cut(s) 461, 492, 1067, 1238
HgaI GACGC 2 cut(s) 302, 871
HhaI GCGC 1 cut(s) 834
Hin1I GRCGYC 2 cut(s) 294, 882
Hin6I GCGC 1 cut(s) 832
HinP1I GCGC 1 cut(s) 832
HinfI GANTC 6 cut(s) 14, 184, 396, 553, 901, 1234
HpaII CCGG 4 cut(s) 461, 492, 1067, 1238
HphI GGTGA 2 cut(s) 400, 958
Hpy166II GTNNAC 1 cut(s) 489
Hpy188I TCNGA 2 cut(s) 612, 622
Hpy188III TCNNGA 5 cut(s) 290, 393, 566, 898, 905
Hpy8I GTNNAC 1 cut(s) 489
Hpy99I CGWCG 2 cut(s) 278, 887
HpyAV CCTTC 2 cut(s) 1333, 1373
HpyCH4III ACNGT 5 cut(s) 286, 703, 827, 877, 1075
HpyCH4IV ACGT 3 cut(s) 202, 308, 595
HpyCH4V TGCA 6 cut(s) 122, 237, 479, 982, 1016, 1084
HpyF10VI GCNNNNNNNGC 3 cut(s) 429, 438, 1090
HpyF3I CTNAG 5 cut(s) 79, 154, 619, 1001, 1245
HpySE526I ACGT 3 cut(s) 202, 308, 595
Hsp92I GRCGYC 2 cut(s) 294, 882
HspAI GCGC 1 cut(s) 832
Kzo9I GATC 3 cut(s) 164, 562, 568
Lsp1109I GCAGC 2 cut(s) 1203, 1253
LweI GCATC 1 cut(s) 224
MaeI CTAG 3 cut(s) 578, 788, 1097
MaeII ACGT 3 cut(s) 202, 308, 595
MalI GATC 3 cut(s) 166, 564, 570
MbiI CCGCTC 1 cut(s) 847
MboI GATC 3 cut(s) 164, 562, 568
MboII GAAGA 3 cut(s) 136, 493, 1291
MfeI CAATTG 1 cut(s) 465
MflI RGATCY 1 cut(s) 568
MhlI GDGCHC 2 cut(s) 408, 648
MlsI TGGCCA 1 cut(s) 804
MluNI TGGCCA 1 cut(s) 804
MlyI GAGTC 1 cut(s) 23
MmeI TCCRAC 2 cut(s) 472, 1290
Mox20I TGGCCA 1 cut(s) 804
MroXI GAANNNNTTC 1 cut(s) 108
MscI TGGCCA 1 cut(s) 804
MseI TTAA 4 cut(s) 72, 749, 863, 1230
MslI CAYNNNNRTG 2 cut(s) 777, 840
Msp20I TGGCCA 1 cut(s) 804
MspI CCGG 4 cut(s) 461, 492, 1067, 1238
MspR9I CCNGG 4 cut(s) 429, 461, 1068, 1137
MunI CAATTG 1 cut(s) 465
MvaI CCWGG 2 cut(s) 429, 1137
MwoI GCNNNNNNNGC 3 cut(s) 429, 438, 1090
NciI CCSGG 2 cut(s) 461, 1068
NcoI CCATGG 1 cut(s) 1266
NdeI CATATG 1 cut(s) 991
NdeII GATC 3 cut(s) 164, 562, 568
NlaIV GGNNCC 1 cut(s) 324
NmeAIII GCCGAG 1 cut(s) 914
NspI RCATGY 1 cut(s) 986
NspV TTCGAA 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 823
PdmI GAANNNNTTC 1 cut(s) 108
PfeI GAWTC 5 cut(s) 184, 396, 553, 901, 1234
PflFI GACNNNGTC 1 cut(s) 388
PflMI CCANNNNNTGG 1 cut(s) 1018
PkrI GCNGC 8 cut(s) 179, 849, 1039, 1065, 1095, 1193, 1243, 1345
PleI GAGTC 1 cut(s) 22
PmaCI CACGTG 1 cut(s) 596
PmlI CACGTG 1 cut(s) 596
PpsI GAGTC 1 cut(s) 22
Ppu21I YACGTR 1 cut(s) 596
PshBI ATTAAT 1 cut(s) 72
PsiI TTATAA 1 cut(s) 656
Psp124BI GAGCTC 1 cut(s) 408
Psp6I CCWGG 2 cut(s) 427, 1135
PspCI CACGTG 1 cut(s) 596
PspGI CCWGG 2 cut(s) 427, 1135
PspN4I GGNNCC 1 cut(s) 324
PspPI GGNCC 9 cut(s) 278, 322, 332, 422, 431, 972, 1039, 1253, 1263
PsuI RGATCY 1 cut(s) 568
PsyI GACNNNGTC 1 cut(s) 388
RsaI GTAC 3 cut(s) 488, 617, 1160
RsaNI GTAC 3 cut(s) 487, 616, 1159
RseI CAYNNNNRTG 2 cut(s) 777, 840
SacI GAGCTC 1 cut(s) 408
SaqAI TTAA 4 cut(s) 72, 749, 863, 1230
SatI GCNGC 8 cut(s) 178, 848, 1038, 1064, 1094, 1192, 1242, 1344
Sau3AI GATC 3 cut(s) 164, 562, 568
Sau96I GGNCC 9 cut(s) 278, 322, 332, 422, 431, 972, 1039, 1253, 1263
SchI GAGTC 1 cut(s) 23
ScrFI CCNGG 4 cut(s) 429, 461, 1068, 1137
SduI GDGCHC 2 cut(s) 408, 648
SfaNI GCATC 1 cut(s) 224
SfcI CTRYAG 2 cut(s) 629, 1168
SfiI GGCCNNNNNGGCC 1 cut(s) 429
SfuI TTCGAA 1 cut(s) 102
SinI GGWCC 4 cut(s) 322, 972, 1253, 1263
SmiMI CAYNNNNRTG 2 cut(s) 777, 840
SmlI CTYRAG 1 cut(s) 1289
SmoI CTYRAG 1 cut(s) 1289
SsiI CCGC 8 cut(s) 50, 177, 847, 1037, 1063, 1094, 1181, 1344
SspMI CTAG 3 cut(s) 578, 788, 1097
SstI GAGCTC 1 cut(s) 408
StyD4I CCNGG 4 cut(s) 427, 459, 1066, 1135
StyI CCWWGG 3 cut(s) 339, 787, 1266
TaaI ACNGT 5 cut(s) 286, 703, 827, 877, 1075
TaiI ACGT 3 cut(s) 205, 311, 598
TaqI TCGA 7 cut(s) 102, 197, 215, 273, 383, 551, 871
TaqII GACCGA 1 cut(s) 989
TatI WGTACW 1 cut(s) 1158
TauI GCSGC 6 cut(s) 180, 850, 1040, 1066, 1096, 1346
TfiI GAWTC 5 cut(s) 184, 396, 553, 901, 1234
Tru1I TTAA 4 cut(s) 72, 749, 863, 1230
Tru9I TTAA 4 cut(s) 72, 749, 863, 1230
TscAI CASTG 3 cut(s) 481, 771, 1023
TseI GCWGC 2 cut(s) 1191, 1241
TspDTI ATGAA 9 cut(s) 17, 259, 514, 1019, 1048, 1069, 1143, 1292, 1317
TspGWI ACGGA 1 cut(s) 1224
TspRI CASTG 3 cut(s) 481, 771, 1023
Tth111I GACNNNGTC 1 cut(s) 388
Van91I CCANNNNNTGG 1 cut(s) 1018
VpaK11BI GGWCC 4 cut(s) 322, 972, 1253, 1263
VspI ATTAAT 1 cut(s) 72
XapI RAATTY 2 cut(s) 85, 379
XceI RCATGY 1 cut(s) 986
XcmI CCANNNNNNNNNTGG 2 cut(s) 1019, 1366
XmaJI CCTAGG 1 cut(s) 787
XmnI GAANNNNTTC 1 cut(s) 108
XspI CTAG 3 cut(s) 578, 788, 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.