FvH4_4g30540

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001444
Physical Location & Seq
Forward (+)
30107093 .. 30109662
2570 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30540.t1

Sequence Viewer

Length: 1380 bp
ATGAAAAAGAAAGAGTCGATAGCGGCGGTTTTGGTGGTGTCGCTCTTGGTGATGTTGTTTGTTTGCCACATCAATCTGTCGGAAAATTTGAAGTTGAACTTCGAACTCATTCAATCACTTGCAACAACTCCAAAACCAGGTATCTTCAAACGATTAGCACCGAGAGGGCAGGACCCTATCGAATCGACACATTTGCAATCTTCATCATCGACAATGATCAGATGCACCCGTTCCGATGATTACTACGATTTCTGTTACATCAACGGCCCAACAGTTCTGGACCCAACAACCTCCACCTTATTCGTAATGGATGCCACAGGCCCAACCGTGGTGGAGAAAATCAAGCCCTACCCACGGAAACACGAGAATTCAACAATGTCGAGAATCAAAGAGTTCACCCTCACCTCCGGCCCACCAGGCCCAAAATGCCAGATCCAACACAATGCCCCGGCCATCGTATTCAGTGCGGGCGGGTACACCGGAAACTTCTTCCATGATTTCAACGATGGGTTCATCCCCCTGTACATCATCGTCAATTCTATCCTCTACGATCAAGTCCCCGTCCTTGCGATTTCCAGAGCACGTGATTGGTGGATTCAGAGGTACTCAGATTTGCTAAACGTGTTTAGCGAGCACCCAATCATAAACCTTGATAATGATACTGCCACACATTGTTTTCGTTCTGCCACCGTGGGCCTAATTGCTCATGGGTTTTTGACCGTAAACCCCAAAAAGTTGAAGACCCCCAAAACACTACCACATTTCCATGCCATGCTAGGAAAAGCCTTTGGCCAAAGGGAAACCGCACAACGATTCCACCTTCAGCGCCACCACAACGATCGGCCACGCCTCGTGTTCATTAGCCGAAACGTAAGCGTGGGCCGAGCAATCTTGAATCAAGAGGAAGCAATGGACTTGGCAGAAAGAATAGGGTTTGAGGTGATTGTTTATGAGCCAAGTTCAAGGACTAGTTTGCATGCAGCATATGACTTGCTTAATAATAGCCATGCGGTGGTTGGTGTTCATGGTGCGGCCCTTACTCACTTGTTGTTCATGCGGCCGGGGACCGTGTTCATGCAAATTGTGCCGCTAGGGACTAAGTGGATAGCAAAATGGTGCTATGAAAATCCAGCTAGAGCAATGGGGCTGCAGTACATTGACTATAGGATTACCGCGGAGGAGAGCAGCTTGGCTGAAACATACGGCAAGGATAACATTGTGGTTAAGGATCCGGCCGGTAAGATTGGTCGGAATTGGTCCGTGGAGTTTATGAAGATTTATCTCAAGGAACAGAATGTCAGGTTGGATTTGGACAGGTTTTCTTGGTTTTTGAAGGCCGCTTATGACAAGGCCAAAAGGTTTATGGAGAAGGAAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

460

Amino Acids

52.03

Weight (kDa)

9.09

Isoelectric Point (pI)

29.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 164 - 358 9.3e-16 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1012
AccII CGCG 1 cut(s) 1175
AclWI GGATC 3 cut(s) 427, 1223, 1236
AcoI YGGCCR 5 cut(s) 450, 790, 842, 1058, 1233
AcsI RAATTY 2 cut(s) 85, 367
AcuI CTGAAG 1 cut(s) 806
AcvI CACGTG 1 cut(s) 584
AfaI GTAC 4 cut(s) 476, 524, 605, 1154
AfiI CCNNNNNNNGG 4 cut(s) 137, 328, 354, 1012
AflIII ACRYGT 1 cut(s) 621
AhlI ACTAGT 1 cut(s) 968
AjnI CCWGG 2 cut(s) 136, 415
AjuI GAANNNNNNNTTGG 2 cut(s) 1286, 1318
AluBI AGCT 2 cut(s) 1133, 1188
AluI AGCT 2 cut(s) 1133, 1188
Alw21I GWGCWC 2 cut(s) 583, 636
AlwI GGATC 3 cut(s) 427, 1223, 1236
ApeKI GCWGC 3 cut(s) 980, 1147, 1185
ApoI RAATTY 2 cut(s) 85, 367
Asp700I GAANNNNTTC 1 cut(s) 108
AspLEI GCGC 1 cut(s) 828
AsuC2I CCSGG 2 cut(s) 449, 1062
AsuHPI GGTGA 4 cut(s) 61, 388, 394, 952
AsuII TTCGAA 1 cut(s) 102
AvaII GGWCC 4 cut(s) 172, 280, 1065, 1257
BalI TGGCCA 1 cut(s) 792
BamHI GGATCC 1 cut(s) 1228
BauI CACGAG 2 cut(s) 362, 851
BbrPI CACGTG 1 cut(s) 584
BbsI GAAGAC 1 cut(s) 746
Bbv12I GWGCWC 2 cut(s) 583, 636
BbvI GCAGC 3 cut(s) 992, 1134, 1197
BccI CCATC 2 cut(s) 461, 500
BceAI ACGGC 2 cut(s) 280, 1219
BcgI CGANNNNNNTGC 2 cut(s) 175, 209
BciT130I CCWGG 2 cut(s) 138, 417
BclI TGATCA 1 cut(s) 216
BcnI CCSGG 2 cut(s) 449, 1062
BcuI ACTAGT 1 cut(s) 968
BfaI CTAG 4 cut(s) 776, 969, 1091, 1134
BfmI CTRYAG 2 cut(s) 1148, 1162
BfoI RGCGCY 1 cut(s) 829
BglI GCCNNNNNGGC 1 cut(s) 417
BisI GCNGC 8 cut(s) 24, 981, 1032, 1058, 1088, 1148, 1186, 1338
BlsI GCNGC 8 cut(s) 25, 982, 1033, 1059, 1089, 1149, 1187, 1339
Bme1390I CCNGG 4 cut(s) 138, 417, 449, 1062
Bme18I GGWCC 4 cut(s) 172, 280, 1065, 1257
BmiI GGNNCC 4 cut(s) 174, 282, 1066, 1230
BmrFI CCNGG 4 cut(s) 138, 417, 449, 1062
BmsI GCATC 2 cut(s) 212, 301
BpiI GAAGAC 1 cut(s) 746
Bpu14I TTCGAA 1 cut(s) 102
BpuEI CTTGAG 1 cut(s) 1268
BpuMI CCSGG 2 cut(s) 449, 1062
BsaAI YACGTR 1 cut(s) 584
BsaJI CCNNGG 7 cut(s) 327, 353, 447, 690, 1061, 1173, 1260
BsaWI WCCGGW 1 cut(s) 479
Bsc4I CCNNNNNNNGG 4 cut(s) 137, 328, 354, 1012
Bse118I RCCGGY 1 cut(s) 1235
Bse3DI GCAATG 2 cut(s) 915, 1146
BseBI CCWGG 2 cut(s) 138, 417
BseDI CCNNGG 7 cut(s) 327, 353, 447, 690, 1061, 1173, 1260
BseGI GGATG 2 cut(s) 316, 513
BseLI CCNNNNNNNGG 4 cut(s) 137, 328, 354, 1012
BseMI GCAATG 2 cut(s) 915, 1146
BseMII CTCAG 1 cut(s) 621
BseRI GAGGAG 1 cut(s) 1193
BseX3I CGGCCG 2 cut(s) 1058, 1233
BseXI GCAGC 3 cut(s) 992, 1134, 1197
Bsh1236I CGCG 1 cut(s) 1175
Bsh1285I CGRYCG 3 cut(s) 841, 1061, 1236
BsiEI CGRYCG 3 cut(s) 841, 1061, 1236
BsiHKAI GWGCWC 2 cut(s) 583, 636
BsiSI CCGG 6 cut(s) 408, 449, 480, 1061, 1232, 1236
BslFI GGGAC 3 cut(s) 542, 1078, 1108
BslI CCNNNNNNNGG 4 cut(s) 137, 328, 354, 1012
BsmFI GGGAC 3 cut(s) 542, 1078, 1108
Bsp119I TTCGAA 1 cut(s) 102
Bsp1286I GDGCHC 2 cut(s) 583, 636
Bsp1407I TGTACA 1 cut(s) 522
Bsp143I GATC 5 cut(s) 216, 432, 550, 838, 1228
BspCNI CTCAG 1 cut(s) 620
BspFNI CGCG 1 cut(s) 1175
BspLI GGNNCC 4 cut(s) 174, 282, 1066, 1230
BspMAI CTGCAG 1 cut(s) 1152
BspPI GGATC 3 cut(s) 427, 1223, 1236
BspT104I TTCGAA 1 cut(s) 102
BsrDI GCAATG 2 cut(s) 915, 1146
BsrFI RCCGGY 1 cut(s) 1235
BsrGI TGTACA 1 cut(s) 522
BssAI RCCGGY 1 cut(s) 1235
BssECI CCNNGG 7 cut(s) 327, 353, 447, 690, 1061, 1173, 1260
BssMI GATC 5 cut(s) 216, 432, 550, 838, 1228
BssSI CACGAG 2 cut(s) 362, 851
Bst2BI CACGAG 2 cut(s) 362, 851
Bst2UI CCWGG 2 cut(s) 138, 417
Bst4CI ACNGT 5 cut(s) 274, 328, 691, 721, 1069
BstAPI GCANNNNNTGC 1 cut(s) 1084
BstAUI TGTACA 1 cut(s) 522
BstBAI YACGTR 1 cut(s) 584
BstBI TTCGAA 1 cut(s) 102
BstC8I GCNNGC 3 cut(s) 469, 632, 978
BstDEI CTNAG 2 cut(s) 607, 1098
BstDSI CCRYGG 5 cut(s) 327, 353, 690, 1173, 1260
BstF5I GGATG 2 cut(s) 316, 513
BstFNI CGCG 1 cut(s) 1175
BstH2I RGCGCY 1 cut(s) 829
BstHHI GCGC 1 cut(s) 828
BstKTI GATC 5 cut(s) 219, 435, 553, 841, 1231
BstMBI GATC 5 cut(s) 216, 432, 550, 838, 1228
BstMCI CGRYCG 3 cut(s) 841, 1061, 1236
BstMWI GCNNNNNNNGC 3 cut(s) 417, 426, 1084
BstNI CCWGG 2 cut(s) 138, 417
BstNSI RCATGY 1 cut(s) 980
BstSCI CCNGG 4 cut(s) 136, 415, 447, 1060
BstSFI CTRYAG 2 cut(s) 1148, 1162
BstUI CGCG 1 cut(s) 1175
BstV1I GCAGC 3 cut(s) 992, 1134, 1197
BstV2I GAAGAC 1 cut(s) 746
BstX2I RGATCY 2 cut(s) 432, 1228
BstYI RGATCY 2 cut(s) 432, 1228
BstZI CGGCCG 2 cut(s) 1058, 1233
BtgI CCRYGG 5 cut(s) 327, 353, 690, 1173, 1260
BtsCI GGATG 2 cut(s) 316, 513
BtsIMutI CAGTG 1 cut(s) 469
Cac8I GCNNGC 3 cut(s) 469, 632, 978
CfoI GCGC 1 cut(s) 828
Cfr10I RCCGGY 1 cut(s) 1235
Cfr42I CCGCGG 1 cut(s) 1176
CsiI ACCWGGT 1 cut(s) 136
Csp6I GTAC 4 cut(s) 475, 523, 604, 1153
CspCI CAANNNNNGTGG 2 cut(s) 312, 347
CviAII CATG 9 cut(s) 494, 707, 767, 772, 977, 1007, 1025, 1054, 1075
CviQI GTAC 4 cut(s) 475, 523, 604, 1153
DdeI CTNAG 2 cut(s) 607, 1098
DpnI GATC 5 cut(s) 218, 434, 552, 840, 1230
DpnII GATC 5 cut(s) 216, 432, 550, 838, 1228
EaeI YGGCCR 5 cut(s) 450, 790, 842, 1058, 1233
EagI CGGCCG 2 cut(s) 1058, 1233
EclXI CGGCCG 2 cut(s) 1058, 1233
Eco47I GGWCC 4 cut(s) 172, 280, 1065, 1257
Eco52I CGGCCG 2 cut(s) 1058, 1233
Eco57I CTGAAG 1 cut(s) 806
Eco72I CACGTG 1 cut(s) 584
EcoO109I RGGNCCY 1 cut(s) 172
EcoRI GAATTC 1 cut(s) 367
EcoRII CCWGG 2 cut(s) 136, 415
FaeI CATG 9 cut(s) 497, 710, 770, 775, 980, 1010, 1028, 1057, 1078
FalI AAGNNNNNCTT 2 cut(s) 83, 115
FaqI GGGAC 3 cut(s) 542, 1078, 1108
FatI CATG 9 cut(s) 493, 706, 766, 771, 976, 1006, 1024, 1053, 1074
FauI CCCGC 2 cut(s) 460, 464
FauNDI CATATG 1 cut(s) 985
FbaI TGATCA 1 cut(s) 216
Fnu4HI GCNGC 8 cut(s) 24, 981, 1032, 1058, 1088, 1148, 1186, 1338
FokI GGATG 2 cut(s) 323, 500
Fsp4HI GCNGC 8 cut(s) 24, 981, 1032, 1058, 1088, 1148, 1186, 1338
FspBI CTAG 4 cut(s) 776, 969, 1091, 1134
GlaI GCGC 1 cut(s) 827
GluI GCNGC 8 cut(s) 24, 981, 1032, 1058, 1088, 1148, 1186, 1338
HaeII RGCGCY 1 cut(s) 829
HapII CCGG 6 cut(s) 408, 449, 480, 1061, 1232, 1236
HhaI GCGC 1 cut(s) 828
Hin1II CATG 9 cut(s) 497, 710, 770, 775, 980, 1010, 1028, 1057, 1078
Hin6I GCGC 1 cut(s) 826
HinP1I GCGC 1 cut(s) 826
HinfI GANTC 6 cut(s) 14, 182, 384, 595, 813, 895
HpaII CCGG 6 cut(s) 408, 449, 480, 1061, 1232, 1236
HphI GGTGA 4 cut(s) 61, 388, 394, 952
Hpy166II GTNNAC 3 cut(s) 396, 477, 724
Hpy188I TCNGA 6 cut(s) 82, 221, 235, 600, 610, 1251
Hpy188III TCNNGA 5 cut(s) 278, 381, 576, 892, 899
Hpy8I GTNNAC 3 cut(s) 396, 477, 724
HpyAV CCTTC 4 cut(s) 830, 1327, 1363, 1367
HpyCH4III ACNGT 5 cut(s) 274, 328, 691, 721, 1069
HpyCH4IV ACGT 3 cut(s) 583, 621, 870
HpyCH4V TGCA 7 cut(s) 122, 196, 225, 976, 980, 1078, 1150
HpyF10VI GCNNNNNNNGC 3 cut(s) 417, 426, 1084
HpyF3I CTNAG 2 cut(s) 607, 1098
HpySE526I ACGT 3 cut(s) 583, 621, 870
Hsp92II CATG 9 cut(s) 497, 710, 770, 775, 980, 1010, 1028, 1057, 1078
HspAI GCGC 1 cut(s) 826
Ksp22I TGATCA 1 cut(s) 216
KspI CCGCGG 1 cut(s) 1176
Kzo9I GATC 5 cut(s) 216, 432, 550, 838, 1228
Lsp1109I GCAGC 3 cut(s) 992, 1134, 1197
LweI GCATC 2 cut(s) 212, 301
MabI ACCWGGT 1 cut(s) 136
MaeI CTAG 4 cut(s) 776, 969, 1091, 1134
MaeII ACGT 3 cut(s) 583, 621, 870
MaeIII GTNAC 1 cut(s) 254
MalI GATC 5 cut(s) 218, 434, 552, 840, 1230
MboI GATC 5 cut(s) 216, 432, 550, 838, 1228
MboII GAAGA 5 cut(s) 136, 192, 481, 751, 1285
MflI RGATCY 2 cut(s) 432, 1228
MhlI GDGCHC 2 cut(s) 583, 636
MlsI TGGCCA 1 cut(s) 792
MluCI AATT 6 cut(s) 85, 367, 535, 699, 1080, 1252
MluNI TGGCCA 1 cut(s) 792
MlyI GAGTC 1 cut(s) 23
MmeI TCCRAC 4 cut(s) 60, 460, 1229, 1284
Mox20I TGGCCA 1 cut(s) 792
MroXI GAANNNNTTC 1 cut(s) 108
MscI TGGCCA 1 cut(s) 792
MseI TTAA 2 cut(s) 996, 1224
MslI CAYNNNNRTG 1 cut(s) 765
Msp20I TGGCCA 1 cut(s) 792
MspA1I CMGCKG 1 cut(s) 1175
MspI CCGG 6 cut(s) 408, 449, 480, 1061, 1232, 1236
MspR9I CCNGG 4 cut(s) 138, 417, 449, 1062
MvaI CCWGG 2 cut(s) 138, 417
MvnI CGCG 1 cut(s) 1175
MwoI GCNNNNNNNGC 3 cut(s) 417, 426, 1084
NciI CCSGG 2 cut(s) 449, 1062
NdeI CATATG 1 cut(s) 985
NdeII GATC 5 cut(s) 216, 432, 550, 838, 1228
NlaIII CATG 9 cut(s) 497, 710, 770, 775, 980, 1010, 1028, 1057, 1078
NlaIV GGNNCC 4 cut(s) 174, 282, 1066, 1230
NmeAIII GCCGAG 1 cut(s) 908
NspI RCATGY 1 cut(s) 980
NspV TTCGAA 1 cut(s) 102
PaeI GCATGC 1 cut(s) 980
PcsI WCGNNNNNNNCGW 1 cut(s) 627
PdmI GAANNNNTTC 1 cut(s) 108
PfeI GAWTC 5 cut(s) 182, 384, 595, 813, 895
PflMI CCANNNNNTGG 1 cut(s) 1012
PkrI GCNGC 8 cut(s) 25, 982, 1033, 1059, 1089, 1149, 1187, 1339
Ple19I CGATCG 1 cut(s) 841
PleI GAGTC 1 cut(s) 22
PmaCI CACGTG 1 cut(s) 584
PmlI CACGTG 1 cut(s) 584
PpsI GAGTC 1 cut(s) 22
Ppu21I YACGTR 1 cut(s) 584
PpuMI RGGWCCY 1 cut(s) 172
Psp5II RGGWCCY 1 cut(s) 172
Psp6I CCWGG 2 cut(s) 136, 415
PspCI CACGTG 1 cut(s) 584
PspGI CCWGG 2 cut(s) 136, 415
PspN4I GGNNCC 4 cut(s) 174, 282, 1066, 1230
PspPPI RGGWCCY 1 cut(s) 172
PstI CTGCAG 1 cut(s) 1152
PsuI RGATCY 2 cut(s) 432, 1228
PvuI CGATCG 1 cut(s) 841
RsaI GTAC 4 cut(s) 476, 524, 605, 1154
RsaNI GTAC 4 cut(s) 475, 523, 604, 1153
RseI CAYNNNNRTG 1 cut(s) 765
SacII CCGCGG 1 cut(s) 1176
SaqAI TTAA 2 cut(s) 996, 1224
SatI GCNGC 8 cut(s) 24, 981, 1032, 1058, 1088, 1148, 1186, 1338
Sau3AI GATC 5 cut(s) 216, 432, 550, 838, 1228
SchI GAGTC 1 cut(s) 23
ScrFI CCNGG 4 cut(s) 138, 417, 449, 1062
SduI GDGCHC 2 cut(s) 583, 636
SexAI ACCWGGT 1 cut(s) 136
SfaNI GCATC 2 cut(s) 212, 301
SfcI CTRYAG 2 cut(s) 1148, 1162
SfiI GGCCNNNNNGGCC 1 cut(s) 417
Sfr303I CCGCGG 1 cut(s) 1176
SfuI TTCGAA 1 cut(s) 102
SgrBI CCGCGG 1 cut(s) 1176
SinI GGWCC 4 cut(s) 172, 280, 1065, 1257
SmiMI CAYNNNNRTG 1 cut(s) 765
SmlI CTYRAG 1 cut(s) 1283
SmoI CTYRAG 1 cut(s) 1283
SpeI ACTAGT 1 cut(s) 968
SphI GCATGC 1 cut(s) 980
Sse9I AATT 6 cut(s) 85, 367, 535, 699, 1080, 1252
SspMI CTAG 4 cut(s) 776, 969, 1091, 1134
StyD4I CCNGG 4 cut(s) 136, 415, 447, 1060
TaaI ACNGT 5 cut(s) 274, 328, 691, 721, 1069
TaiI ACGT 3 cut(s) 586, 624, 873
TaqI TCGA 6 cut(s) 17, 102, 180, 185, 209, 380
TasI AATT 6 cut(s) 85, 367, 535, 699, 1080, 1252
TatI WGTACW 2 cut(s) 522, 1152
TauI GCSGC 5 cut(s) 26, 1034, 1060, 1090, 1340
TfiI GAWTC 5 cut(s) 182, 384, 595, 813, 895
Tru1I TTAA 2 cut(s) 996, 1224
Tru9I TTAA 2 cut(s) 996, 1224
TscAI CASTG 1 cut(s) 469
TseI GCWGC 3 cut(s) 980, 1147, 1185
TspDTI ATGAA 9 cut(s) 17, 192, 502, 847, 1013, 1042, 1063, 1137, 1286
TspGWI ACGGA 2 cut(s) 370, 1249
TspRI CASTG 1 cut(s) 469
Van91I CCANNNNNTGG 1 cut(s) 1012
VpaK11BI GGWCC 4 cut(s) 172, 280, 1065, 1257
XapI RAATTY 2 cut(s) 85, 367
XceI RCATGY 1 cut(s) 980
XcmI CCANNNNNNNNNTGG 2 cut(s) 1013, 1360
XmnI GAANNNNTTC 1 cut(s) 108
XspI CTAG 4 cut(s) 776, 969, 1091, 1134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.