AT2G04060

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001959
Physical Location & Seq
Reverse (-)
1342137 .. 1345164
3028 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G04060.1

Sequence Viewer

Length: 1410 bp
ATGACTAATTCTCTTGATGTTGGTGTTCCATGGATCATGTGTCAACAAGATGATGCTCCTCAACCAATGTATCATGGACATACCAACTTTGATAGAACCGCGGGTGGTCCGTTCATCACAACAACATATGATTACGATGCTCCTCTTGACGAATTTGGTAATTTGAACCAACCAAAATACGGACACCTGAAACAGCTTCATGATGTTTTTCATGCTATGGAGAAAACTCTCACTTATGGAAATATTTCCACCGCTGATTTTGGAAATTTAGTCATGACAACGGTTTATCAAACAGAAGAAGGATCAAGTTGTTTCATTGGAAACGTGAATGCAAAAATCAATTTCCAAGGAACATCTTATGATGTCCCGGCTTGGTATGTGAGCATTTTACCGGATTGCAAAACTGAGTCTTATAATACCGCCAAGCGAATGAAGCTGAGAACGAGCCTTCGATTTAAAAATGTAAGCAATGACGAAAGTGATTTTCTTTGGTACATGACTACGGTTAATCTCAAAGAACAAGATCCGGCTTGGGGAAAAAATATGTCTCTTCGTATCAACAGTACTGCTCATGTTCTTCACGGTTTTGTCAATGGACAACATACTGGTAATTATCGCGTTGAAAATGGAAAGTTTCACTATGTTTTTGAGCAAGACGCGAAGTTCAACCCTGGTGTTAATGTCATTACCCTCCTTAGCGTAACCGTAGACCTTCCGAACTATGGTGCTTTCTTCGAAAACGTCCCGGCTGGAATCACTGGACCCGTTTTTATCATTGGAAGAAATGGTGATGAAACTGTTGTAAAATATTTGTCTACTCATAATGGAGCTACAAAACTGACTATTTTCAAAGCTCCTTTGGGAAGTGAACCAGTTGTGGTCGATCTTTTGGGATTTGGAAAAGGAAAGGCTTCGATCAATGAAAATTACACTGGCCGTTACTGGCCAGATCCACCTGTTCTGTTACGGTTTTCCACCCATCTCTTGCATAACTGGGTTCTAGCTTCCTCTCCATATCGCTACGTAATGTCTGAGCTCATCTCTCAATATGCGAAAAACCTAGCAATCATCCTCAAGCATCTAAGCAATATTACCCTTGCTCGAACCCGTGTCCAAGCACTTTCTCGTGTAGTTCTCTTCGGGCCAGTGAGATTCACGACTTTGACCACTTTTACAACGCCACTTGGTCTCTTGACAATGGCTATATGTTCATCCATTGACTCGTCTTTGGAAGAATTGTCGATAATCTTTGATCTCACATGTACCAAAATGCTTTATAGCTTTTGGCTCAAAGCTCTCAAAGAACTCTTGTCGATTAAACCTATCGATCCTTTTATCTATCTTTTTATCTACCTTATGTTGGCTTTGTGGAATGCTCCTTATGGTTGTATTGTTAACTTCTGGATCTGA

Protein Analysis

469

Amino Acids

52.85

Weight (kDa)

6.67

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 23 - 68 1.4e-12 Glycosyl hydrolases family 35
GHD PF17834 94 - 142 1.8e-15 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 163 - 227 6.7e-06 Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd PF21467 281 - 319 4.5e-06 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 414
AccI GTMKAC 2 cut(s) 708, 815
AccII CGCG 3 cut(s) 101, 618, 659
AciI CCGC 4 cut(s) 99, 101, 252, 420
AclWI GGATC 5 cut(s) 41, 310, 518, 944, 1322
AcoI YGGCCR 2 cut(s) 934, 944
AcsI RAATTY 2 cut(s) 152, 265
AfaI GTAC 3 cut(s) 494, 565, 1264
AfiI CCNNNNNNNGG 4 cut(s) 179, 533, 722, 1360
AflIII ACRYGT 1 cut(s) 1259
AgsI TTSAA 4 cut(s) 166, 623, 667, 850
AjnI CCWGG 1 cut(s) 670
AluBI AGCT 8 cut(s) 196, 436, 830, 854, 1004, 1036, 1281, 1295
AluI AGCT 8 cut(s) 196, 436, 830, 854, 1004, 1036, 1281, 1295
Alw21I GWGCWC 1 cut(s) 1038
Alw26I GTCTC 2 cut(s) 552, 1193
AlwI GGATC 5 cut(s) 41, 310, 518, 944, 1322
AoxI GGCC 3 cut(s) 934, 944, 1142
ApoI RAATTY 2 cut(s) 152, 265
Asp700I GAANNNNTTC 2 cut(s) 244, 910
AspS9I GGNCC 3 cut(s) 107, 761, 1142
AsuC2I CCSGG 2 cut(s) 368, 746
AsuHPI GGTGA 1 cut(s) 800
AsuII TTCGAA 1 cut(s) 735
AvaII GGWCC 2 cut(s) 107, 761
BaeI ACNNNNGTAYC 2 cut(s) 53, 86
BalI TGGCCA 1 cut(s) 946
BanII GRGCYC 1 cut(s) 1038
BauI CACGAG 1 cut(s) 1125
Bbv12I GWGCWC 1 cut(s) 1038
BccI CCATC 1 cut(s) 987
BceAI ACGGC 1 cut(s) 921
BciT130I CCWGG 1 cut(s) 672
BcnI CCSGG 2 cut(s) 368, 746
BcoDI GTCTC 2 cut(s) 552, 1193
BfaI CTAG 2 cut(s) 1001, 1061
BmcAI AGTACT 1 cut(s) 565
Bme1390I CCNGG 3 cut(s) 368, 672, 746
Bme18I GGWCC 2 cut(s) 107, 761
BmgT120I GGNCC 3 cut(s) 107, 761, 1142
BmiI GGNNCC 1 cut(s) 763
BmrFI CCNGG 3 cut(s) 368, 672, 746
BmrI ACTGGG 1 cut(s) 1003
BmsI GCATC 3 cut(s) 43, 127, 1087
BmuI ACTGGG 1 cut(s) 1003
BplI GAGNNNNNCTC 2 cut(s) 1025, 1057
Bpu10I CCTNAGC 1 cut(s) 695
Bpu14I TTCGAA 1 cut(s) 735
BpuEI CTTGAG 1 cut(s) 1058
BpuMI CCSGG 2 cut(s) 368, 746
Bsa29I ATCGAT 1 cut(s) 1326
BsaAI YACGTR 1 cut(s) 1024
BsaI GGTCTC 1 cut(s) 1193
BsaJI CCNNGG 4 cut(s) 29, 99, 346, 670
BsaWI WCCGGW 1 cut(s) 391
Bsc4I CCNNNNNNNGG 4 cut(s) 179, 533, 722, 1360
Bse1I ACTGG 7 cut(s) 610, 763, 872, 937, 947, 998, 1145
Bse3DI GCAATG 1 cut(s) 475
BseBI CCWGG 1 cut(s) 672
BseCI ATCGAT 1 cut(s) 1326
BseDI CCNNGG 4 cut(s) 29, 99, 346, 670
BseGI GGATG 2 cut(s) 1068, 1211
BseLI CCNNNNNNNGG 4 cut(s) 179, 533, 722, 1360
BseMI GCAATG 1 cut(s) 475
BseMII CTCAG 3 cut(s) 396, 428, 1023
BseNI ACTGG 7 cut(s) 610, 763, 872, 937, 947, 998, 1145
BseRI GAGGAG 2 cut(s) 48, 132
Bsh1236I CGCG 3 cut(s) 101, 618, 659
BshFI GGCC 3 cut(s) 936, 946, 1144
BshVI ATCGAT 1 cut(s) 1326
BsiHKAI GWGCWC 1 cut(s) 1038
BsiSI CCGG 4 cut(s) 368, 392, 527, 746
BslFI GGGAC 2 cut(s) 350, 728
BslI CCNNNNNNNGG 4 cut(s) 179, 533, 722, 1360
BsmAI GTCTC 2 cut(s) 552, 1193
BsmFI GGGAC 2 cut(s) 350, 728
BsmI GAATGC 2 cut(s) 334, 1378
BsnI GGCC 3 cut(s) 936, 946, 1144
Bso31I GGTCTC 1 cut(s) 1193
Bsp119I TTCGAA 1 cut(s) 735
Bsp1286I GDGCHC 1 cut(s) 1038
Bsp143I GATC 9 cut(s) 33, 302, 523, 883, 915, 949, 1252, 1327, 1404
Bsp19I CCATGG 1 cut(s) 29
BspACI CCGC 4 cut(s) 99, 101, 252, 420
BspANI GGCC 3 cut(s) 936, 946, 1144
BspCNI CTCAG 3 cut(s) 397, 429, 1024
BspDI ATCGAT 1 cut(s) 1326
BspFNI CGCG 3 cut(s) 101, 618, 659
BspHI TCATGA 2 cut(s) 199, 273
BspLI GGNNCC 1 cut(s) 763
BspPI GGATC 5 cut(s) 41, 310, 518, 944, 1322
BspT104I TTCGAA 1 cut(s) 735
BspTNI GGTCTC 1 cut(s) 1193
BsrDI GCAATG 1 cut(s) 475
BsrI ACTGG 7 cut(s) 610, 763, 872, 937, 947, 998, 1145
BssECI CCNNGG 4 cut(s) 29, 99, 346, 670
BssMI GATC 9 cut(s) 33, 302, 523, 883, 915, 949, 1252, 1327, 1404
BssSI CACGAG 1 cut(s) 1125
BssT1I CCWWGG 2 cut(s) 29, 346
Bst2BI CACGAG 1 cut(s) 1125
Bst2UI CCWGG 1 cut(s) 672
Bst4CI ACNGT 7 cut(s) 283, 505, 563, 584, 706, 799, 969
Bst6I CTCTTC 2 cut(s) 555, 1142
BstBAI YACGTR 1 cut(s) 1024
BstBI TTCGAA 1 cut(s) 735
BstDEI CTNAG 5 cut(s) 405, 437, 695, 1032, 1082
BstDSI CCRYGG 2 cut(s) 29, 99
BstF5I GGATG 2 cut(s) 1068, 1211
BstFNI CGCG 3 cut(s) 101, 618, 659
BstKTI GATC 9 cut(s) 36, 305, 526, 886, 918, 952, 1255, 1330, 1407
BstMAI GTCTC 2 cut(s) 552, 1193
BstMBI GATC 9 cut(s) 33, 302, 523, 883, 915, 949, 1252, 1327, 1404
BstMWI GCNNNNNNNGC 1 cut(s) 433
BstNI CCWGG 1 cut(s) 672
BstNSI RCATGY 1 cut(s) 1263
BstSCI CCNGG 3 cut(s) 366, 670, 744
BstSNI TACGTA 1 cut(s) 1024
BstUI CGCG 3 cut(s) 101, 618, 659
BstX2I RGATCY 3 cut(s) 523, 949, 1404
BstYI RGATCY 3 cut(s) 523, 949, 1404
Bsu15I ATCGAT 1 cut(s) 1326
BsuRI GGCC 3 cut(s) 936, 946, 1144
BsuTUI ATCGAT 1 cut(s) 1326
BtgI CCRYGG 2 cut(s) 29, 99
BtsCI GGATG 2 cut(s) 1068, 1211
BtsIMutI CAGTG 3 cut(s) 756, 930, 1152
CciI TCATGA 2 cut(s) 199, 273
Cfr13I GGNCC 3 cut(s) 107, 761, 1142
Cfr42I CCGCGG 1 cut(s) 102
ClaI ATCGAT 1 cut(s) 1326
CseI GACGC 1 cut(s) 665
Csp6I GTAC 3 cut(s) 493, 564, 1263
CviAII CATG 9 cut(s) 30, 37, 74, 200, 212, 274, 496, 572, 1260
CviQI GTAC 3 cut(s) 493, 564, 1263
DdeI CTNAG 5 cut(s) 405, 437, 695, 1032, 1082
DpnI GATC 9 cut(s) 35, 304, 525, 885, 917, 951, 1254, 1329, 1406
DpnII GATC 9 cut(s) 33, 302, 523, 883, 915, 949, 1252, 1327, 1404
DraI TTTAAA 1 cut(s) 457
EaeI YGGCCR 2 cut(s) 934, 944
Eam1104I CTCTTC 2 cut(s) 555, 1142
EarI CTCTTC 2 cut(s) 555, 1142
Ecl136II GAGCTC 1 cut(s) 1036
Eco105I TACGTA 1 cut(s) 1024
Eco130I CCWWGG 2 cut(s) 29, 346
Eco24I GRGCYC 1 cut(s) 1038
Eco31I GGTCTC 1 cut(s) 1193
Eco47I GGWCC 2 cut(s) 107, 761
Eco53kI GAGCTC 1 cut(s) 1036
EcoICRI GAGCTC 1 cut(s) 1036
EcoRII CCWGG 1 cut(s) 670
EcoT14I CCWWGG 2 cut(s) 29, 346
EcoT38I GRGCYC 1 cut(s) 1038
ErhI CCWWGG 2 cut(s) 29, 346
FaeI CATG 9 cut(s) 33, 40, 77, 203, 215, 277, 499, 575, 1263
FaqI GGGAC 2 cut(s) 350, 728
FatI CATG 9 cut(s) 29, 36, 73, 199, 211, 273, 495, 571, 1259
FauI CCCGC 1 cut(s) 94
FauNDI CATATG 1 cut(s) 127
FblI GTMKAC 2 cut(s) 708, 815
FokI GGATG 2 cut(s) 1055, 1198
FriOI GRGCYC 1 cut(s) 1038
FspBI CTAG 2 cut(s) 1001, 1061
HaeIII GGCC 3 cut(s) 936, 946, 1144
HapII CCGG 4 cut(s) 368, 392, 527, 746
HgaI GACGC 1 cut(s) 665
Hin1II CATG 9 cut(s) 33, 40, 77, 203, 215, 277, 499, 575, 1263
HincII GTYRAC 2 cut(s) 44, 1396
HindII GTYRAC 2 cut(s) 44, 1396
HinfI GANTC 4 cut(s) 407, 753, 1152, 1220
HpaI GTTAAC 1 cut(s) 1396
HpaII CCGG 4 cut(s) 368, 392, 527, 746
HphI GGTGA 1 cut(s) 800
Hpy166II GTNNAC 5 cut(s) 44, 709, 816, 869, 1396
Hpy188I TCNGA 3 cut(s) 717, 1033, 1409
Hpy188III TCNNGA 7 cut(s) 14, 146, 200, 274, 1156, 1192, 1402
Hpy8I GTNNAC 5 cut(s) 44, 709, 816, 869, 1396
HpyAV CCTTC 3 cut(s) 293, 458, 722
HpyCH4III ACNGT 7 cut(s) 283, 505, 563, 584, 706, 799, 969
HpyCH4IV ACGT 3 cut(s) 324, 741, 1023
HpyCH4V TGCA 3 cut(s) 332, 399, 988
HpyF10VI GCNNNNNNNGC 1 cut(s) 433
HpyF3I CTNAG 5 cut(s) 405, 437, 695, 1032, 1082
HpySE526I ACGT 3 cut(s) 324, 741, 1023
Hsp92II CATG 9 cut(s) 33, 40, 77, 203, 215, 277, 499, 575, 1263
KspAI GTTAAC 1 cut(s) 1396
KspI CCGCGG 1 cut(s) 102
Kzo9I GATC 9 cut(s) 33, 302, 523, 883, 915, 949, 1252, 1327, 1404
LmnI GCTCC 5 cut(s) 61, 145, 827, 859, 1381
LweI GCATC 3 cut(s) 43, 127, 1087
MaeI CTAG 2 cut(s) 1001, 1061
MaeII ACGT 3 cut(s) 324, 741, 1023
MaeIII GTNAC 3 cut(s) 700, 938, 963
MalI GATC 9 cut(s) 35, 304, 525, 885, 917, 951, 1254, 1329, 1406
MboI GATC 9 cut(s) 33, 302, 523, 883, 915, 949, 1252, 1327, 1404
MboII GAAGA 7 cut(s) 308, 542, 569, 724, 792, 1129, 1244
MflI RGATCY 3 cut(s) 523, 949, 1404
MhlI GDGCHC 1 cut(s) 1038
MlsI TGGCCA 1 cut(s) 946
MluCI AATT 8 cut(s) 7, 152, 160, 265, 340, 610, 925, 1235
MluNI TGGCCA 1 cut(s) 946
MlyI GAGTC 2 cut(s) 416, 1214
MnlI CCTC 5 cut(s) 69, 153, 701, 1018, 1082
Mox20I TGGCCA 1 cut(s) 946
MroXI GAANNNNTTC 2 cut(s) 244, 910
MscI TGGCCA 1 cut(s) 946
MseI TTAA 5 cut(s) 456, 507, 678, 1317, 1395
Msp20I TGGCCA 1 cut(s) 946
MspA1I CMGCKG 2 cut(s) 101, 254
MspI CCGG 4 cut(s) 368, 392, 527, 746
MspR9I CCNGG 3 cut(s) 368, 672, 746
Mva1269I GAATGC 2 cut(s) 334, 1378
MvaI CCWGG 1 cut(s) 672
MvnI CGCG 3 cut(s) 101, 618, 659
MwoI GCNNNNNNNGC 1 cut(s) 433
NciI CCSGG 2 cut(s) 368, 746
NcoI CCATGG 1 cut(s) 29
NdeI CATATG 1 cut(s) 127
NdeII GATC 9 cut(s) 33, 302, 523, 883, 915, 949, 1252, 1327, 1404
NlaIII CATG 9 cut(s) 33, 40, 77, 203, 215, 277, 499, 575, 1263
NlaIV GGNNCC 1 cut(s) 763
NspI RCATGY 1 cut(s) 1263
NspV TTCGAA 1 cut(s) 735
PagI TCATGA 2 cut(s) 199, 273
PciI ACATGT 1 cut(s) 1259
PctI GAATGC 2 cut(s) 334, 1378
PdmI GAANNNNTTC 2 cut(s) 244, 910
PfeI GAWTC 2 cut(s) 753, 1152
PleI GAGTC 2 cut(s) 415, 1214
PpsI GAGTC 2 cut(s) 415, 1214
Ppu21I YACGTR 1 cut(s) 1024
PscI ACATGT 1 cut(s) 1259
PsiI TTATAA 1 cut(s) 414
Psp124BI GAGCTC 1 cut(s) 1038
Psp6I CCWGG 1 cut(s) 670
PspGI CCWGG 1 cut(s) 670
PspN4I GGNNCC 1 cut(s) 763
PspPI GGNCC 3 cut(s) 107, 761, 1142
PsuI RGATCY 3 cut(s) 523, 949, 1404
RsaI GTAC 3 cut(s) 494, 565, 1264
RsaNI GTAC 3 cut(s) 493, 564, 1263
SacI GAGCTC 1 cut(s) 1038
SacII CCGCGG 1 cut(s) 102
SaqAI TTAA 5 cut(s) 456, 507, 678, 1317, 1395
Sau3AI GATC 9 cut(s) 33, 302, 523, 883, 915, 949, 1252, 1327, 1404
Sau96I GGNCC 3 cut(s) 107, 761, 1142
ScaI AGTACT 1 cut(s) 565
SchI GAGTC 2 cut(s) 416, 1214
ScrFI CCNGG 3 cut(s) 368, 672, 746
SduI GDGCHC 1 cut(s) 1038
SfaNI GCATC 3 cut(s) 43, 127, 1087
Sfr303I CCGCGG 1 cut(s) 102
SfuI TTCGAA 1 cut(s) 735
SgrBI CCGCGG 1 cut(s) 102
SinI GGWCC 2 cut(s) 107, 761
SmlI CTYRAG 1 cut(s) 1073
SmoI CTYRAG 1 cut(s) 1073
SnaBI TACGTA 1 cut(s) 1024
Sse9I AATT 8 cut(s) 7, 152, 160, 265, 340, 610, 925, 1235
SsiI CCGC 4 cut(s) 99, 101, 252, 420
SspI AATATT 3 cut(s) 244, 809, 1090
SspMI CTAG 2 cut(s) 1001, 1061
SstI GAGCTC 1 cut(s) 1038
StyD4I CCNGG 3 cut(s) 366, 670, 744
StyI CCWWGG 2 cut(s) 29, 346
TaaI ACNGT 7 cut(s) 283, 505, 563, 584, 706, 799, 969
TaiI ACGT 3 cut(s) 327, 744, 1026
TaqI TCGA 8 cut(s) 451, 735, 882, 914, 1102, 1241, 1313, 1326
TasI AATT 8 cut(s) 7, 152, 160, 265, 340, 610, 925, 1235
TatI WGTACW 1 cut(s) 563
TfiI GAWTC 2 cut(s) 753, 1152
Tru1I TTAA 5 cut(s) 456, 507, 678, 1317, 1395
Tru9I TTAA 5 cut(s) 456, 507, 678, 1317, 1395
TscAI CASTG 3 cut(s) 763, 937, 1152
TspDTI ATGAA 8 cut(s) 103, 188, 200, 304, 446, 807, 936, 1200
TspGWI ACGGA 2 cut(s) 99, 195
TspRI CASTG 3 cut(s) 763, 937, 1152
VpaK11BI GGWCC 2 cut(s) 107, 761
XapI RAATTY 2 cut(s) 152, 265
XceI RCATGY 1 cut(s) 1263
XmiI GTMKAC 2 cut(s) 708, 815
XmnI GAANNNNTTC 2 cut(s) 244, 910
XspI CTAG 2 cut(s) 1001, 1061
ZrmI AGTACT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.