Rroxscaffold_2G00146240

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001959
Physical Location & Seq
Reverse (-)
84183188 .. 84188973
5786 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00146240.1

Sequence Viewer

Length: 2532 bp
ATGGCCGGACGCGCCGCCACGCGCCGCCACAAGCGGCGGTCAAACCGGTGGTCAACGGCGGCGGTCACCGCCGGATCAACCACCTCGGATGGCAAAGTGACCAACTACAAACTTGTTGGAAATAAAGACCTCGTTGCTAATGCTACTGACGTTTCTTATGATGGCAGAGCCATCACCATTGATGGCAAACGAAGGATTTTGAATTCTGGTTCCATACATTATCCTCGAAGTACACCACAAATGTGGCCAGACTTGATTAGGAAGTCAAAGGAAGGTGGACTCGACGCAATTGAGACTTATGTCTTCTGGAATGCGCATGAACCAGTTCGTCGTGAATACGATTTTACTGGTAATCTTGACCTGGTTAGGTTTATCAAAACGATTCAAGATGAAGGACTTTATGCTGTTCTTCGGATTGGTCCATATGTTTGTGCTGAATGGAATTATGGAGGACTTCCTGTTTGGCTCCATAACCTGCCAAACTGTCAGATTCGAACAGCAAACGATGTTTTCATGAATGAGATGAAGAATTTCACTACATTGATCGTGGATATGATGAAAAAAGAGAAACTATTTGCATCTCAAGGAGGCCCTATCATTATTGCTCAGATTGAAAATGAATATGGGAATGTGGAATCATACTATGGAGATGCTGGAAAAGCATACATTAACTGGTCTGCAAACTTTGCTGAATCACTAAACATCGGAGTTCCATGGATCATGTGTCAACAAAAAGATGCTCCCGCATCAATGATAAACACATGTAATGGCTGGTATTGTGACACATTTAAGCCAAGTAACCCCAACACGCCTAAGATGTGGACTGAAAATTGGACTGGCTGGTTCAAGAGCTGGGGTGGTTTAGACCCACTCAGAACTGCTGAGGACGTTGCCTTTGCAGTAGCAAGGTTTTTTTTTCAATATGGAGGCACATTCCAAAACTATTACATGTATCATGGTGGAACAAACTTTGGTAGAACAGCAGCTTCATACATTACCACCTCGTATGATTATGATGCCCCTCTTGATGAGTATGGCCATTTGAGTCAACCAAAATGGGGGCATTTGAAAGAGCTTCATGAGGTTTTGAAATCCATGGAATATACTCTCACCTATGGCAATATTACCACCACAGAATTGGGTAACTCTGTCTCGACCACTGTGTTTGCGACAAATGAGTCGAGTAGCTGCATTTTGGGCAATGCAAATAGCAGTTCAGATGCTACAATCACTTTCCAAGGAAACACATACACCATTCCTGCTTGGTCTGTTAGTATTCTTCCAGATTGCAAAAAAGAAGCATACAACACTGCTAAGGTAAACACCCAAACTACAGTGAAAGTAAAAAGTGTCAACAAGGCTGAGGATGAACCTGAATCCCTTCATTGGTCATGGAGGCCCGAGAAGACGGATGACACCCAGCTTCTTGGAAAAGGTGACGTTGTGGCAAGTCATCTTATGGAACAAAAAGAGGCTGCAAATGATGCTAGTGATTATCTTTGGTACATGACAAGTGTTCAACTCAGTAAGGATGATCCAATTTTGAGTGGGAACATGACATTGAGGATAAATGAAACTGGTTACATTCTTCATGCTTATGTAAATGGAGAACATGTTGGTTCTCAATGGGCTGCTTATAACGTCTTCAACTACGTTTTCGAGAAACCTATCAAGTTGGTTCCCGGAGTGAACACAATTTCATTGCTCAGTGCTACTGTTGGATTTCCGAATTATGGAGGTGGATTTGAGGACATCAAAACTGGAGTACCTGGTCCTGTTCAATTGGTTGGACAAAGTGGAGATGAAACTGTGATCAAGGATTTGTCAGAACACAAGTGGTCATACAAGGTTGGACTCCATGGCTTGAAAGACGAGCTCTTTAGCTCTGCTTCTCGTTTTGCAAATAAATGGTCAGTTGAAGGTTTACCAGTAAACAGGATGATGACATGGTATAAGACAACTTTCAAAGCTCCCCTAGGAACTGAACCAGTCGCGGTGGACTTACACGGGTTGGGTAAGGGACATGCTTGGGTGAACGGGCATAGCATTGGGCGATATTGGCCAAGCTACTTGGCTCCAGAGGATGGTTGTAGTGTTGAAGCTTGTGACTACCGTGGTGCATACGACAATAACAAGTGTGTCTTCAACTGTATCATGTTCCGCGATCCTTTTTTGCAAGATGATGAGAACACGTTGGTCTTGTTTGAGGAATTTGGTGGCAACCCATCTTATGTGAATTTCCAGACAATCAGAGTTGGAACTATTTGTGCAAATGCATATGAGAATAATACATTGGAATTGGCATGCCAAGGTCGCCCCATTTCAGCAATCAAATTTGCTAGCTTTGGAAAACCTGAAGGGACTTGTGGATCATTTAAGAAAGGTACCTGTGATGGCACAACTGCTTTGTCCATCCTTCAAAAGGAATGTGTTGGAAAAGAGTCGTGCTCAATTGACGTATCAGAAAAGAAGTTCGGTTCCACAAATTGTGGCGATATTGTGAAGAGGCTGGCCGTGGAAGCAGTTTGTTAG

Protein Analysis

843

Amino Acids

93.73

Weight (kDa)

5.86

Isoelectric Point (pI)

32.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 58 - 361 1e-117 Glycosyl hydrolases family 35
Glyco_hydro_42 PF02449 79 - 228 8.7e-07 Beta-galactosidase
GHD PF17834 386 - 440 8.7e-22 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 648 - 689 2.8e-17 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 768 - 843 3.4e-18 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1638
AasI GACNNNNNNGTC 1 cut(s) 1177
Acc16I TGCGCA 1 cut(s) 315
Acc36I ACCTGC 1 cut(s) 483
Acc65I GGTACC 1 cut(s) 2384
AccB1I GGYRCC 1 cut(s) 2384
AccB7I CCANNNNNTGG 1 cut(s) 2084
AccII CGCG 4 cut(s) 12, 22, 1994, 2163
AclWI GGATC 5 cut(s) 82, 725, 1529, 2159, 2377
AcoI YGGCCR 5 cut(s) 3, 245, 1036, 2060, 2511
AcsI RAATTY 5 cut(s) 202, 529, 2210, 2236, 2333
AcuI CTGAAG 1 cut(s) 2376
AfaI GTAC 4 cut(s) 232, 1505, 1767, 2386
AfiI CCNNNNNNNGG 5 cut(s) 1058, 1386, 1733, 2084, 2422
AflIII ACRYGT 4 cut(s) 761, 948, 1612, 2190
AgeI ACCGGT 1 cut(s) 45
AjnI CCWGG 2 cut(s) 360, 1768
AjuI GAANNNNNNNTTGG 2 cut(s) 2276, 2308
AleI CACNNNNGTG 1 cut(s) 241
Alw21I GWGCWC 2 cut(s) 1878, 2450
Alw26I GTCTC 2 cut(s) 287, 1156
AlwI GGATC 5 cut(s) 82, 725, 1529, 2159, 2377
Ama87I CYCGRG 1 cut(s) 1400
AoxI GGCC 7 cut(s) 3, 245, 589, 1036, 1397, 2060, 2511
ApeKI GCWGC 4 cut(s) 983, 1188, 1475, 1631
ApoI RAATTY 5 cut(s) 202, 529, 2210, 2236, 2333
ArsI GACNNNNNNTTYG 8 cut(s) 35, 67, 444, 476, 878, 910, 1895, 1927
AsiGI ACCGGT 1 cut(s) 45
Asp700I GAANNNNTTC 3 cut(s) 324, 530, 1380
Asp718I GGTACC 1 cut(s) 2384
AspA2I CCTAGG 1 cut(s) 1975
AspLEI GCGC 3 cut(s) 14, 24, 316
AspS9I GGNCC 4 cut(s) 419, 590, 1398, 1772
AsuC2I CCSGG 1 cut(s) 1683
AsuHPI GGTGA 5 cut(s) 58, 166, 1102, 1448, 2044
AsuII TTCGAA 1 cut(s) 493
AsuNHI GCTAGC 1 cut(s) 2339
AvaI CYCGRG 1 cut(s) 1400
AvaII GGWCC 2 cut(s) 419, 1772
AvrII CCTAGG 1 cut(s) 1975
BaeI ACNNNNGTAYC 2 cut(s) 1749, 1782
BalI TGGCCA 3 cut(s) 247, 1038, 2062
BanI GGYRCC 1 cut(s) 2384
BanII GRGCYC 1 cut(s) 1878
BbsI GAAGAC 4 cut(s) 295, 1412, 1636, 2134
Bbv12I GWGCWC 2 cut(s) 1878, 2450
BbvCI CCTCAGC 2 cut(s) 882, 1362
BbvI GCAGC 4 cut(s) 995, 1175, 1462, 1618
BccI CCATC 8 cut(s) 83, 155, 176, 179, 2078, 2233, 2387, 2420
BceAI ACGGC 2 cut(s) 72, 2498
BciT130I CCWGG 2 cut(s) 362, 1770
BclI TGATCA 1 cut(s) 1812
BcnI CCSGG 1 cut(s) 1683
BcoDI GTCTC 2 cut(s) 287, 1156
BfaI CTAG 3 cut(s) 1488, 1976, 2340
BfmI CTRYAG 1 cut(s) 1332
BfuAI ACCTGC 1 cut(s) 483
BisI GCNGC 8 cut(s) 15, 25, 35, 60, 984, 1189, 1476, 1632
BlnI CCTAGG 1 cut(s) 1975
BlsI GCNGC 8 cut(s) 16, 26, 36, 61, 985, 1190, 1477, 1633
Bme1390I CCNGG 3 cut(s) 362, 1683, 1770
Bme18I GGWCC 2 cut(s) 419, 1772
BmeT110I CYCGRG 1 cut(s) 1400
BmgT120I GGNCC 4 cut(s) 419, 590, 1398, 1772
BmiI GGNNCC 6 cut(s) 211, 467, 1680, 2076, 2386, 2479
BmrFI CCNGG 3 cut(s) 362, 1683, 1770
BmsI GCATC 7 cut(s) 587, 640, 727, 755, 1006, 1210, 1474
BmtI GCTAGC 1 cut(s) 2343
BoxI GACNNNNGTC 1 cut(s) 299
BpiI GAAGAC 4 cut(s) 295, 1412, 1636, 2134
BplI GAGNNNNNCTC 2 cut(s) 2432, 2464
BpmI CTGGAG 2 cut(s) 1782, 2061
Bpu10I CCTNAGC 3 cut(s) 882, 1314, 1362
Bpu14I TTCGAA 1 cut(s) 493
BpuEI CTTGAG 1 cut(s) 567
BpuMI CCSGG 1 cut(s) 1683
BsaJI CCNNGG 9 cut(s) 84, 713, 1095, 1237, 1858, 1975, 2113, 2308, 2514
BsaWI WCCGGW 1 cut(s) 45
BsaXI ACNNNNNCTCC 2 cut(s) 1599, 1629
Bsc4I CCNNNNNNNGG 5 cut(s) 1058, 1386, 1733, 2084, 2422
Bse118I RCCGGY 1 cut(s) 45
Bse1I ACTGG 8 cut(s) 323, 352, 677, 841, 1582, 1765, 1928, 1988
Bse3DI GCAATG 2 cut(s) 1207, 1700
BseBI CCWGG 2 cut(s) 362, 1770
BseDI CCNNGG 9 cut(s) 84, 713, 1095, 1237, 1858, 1975, 2113, 2308, 2514
BseGI GGATG 7 cut(s) 94, 1372, 1417, 1537, 1944, 2089, 2412
BseLI CCNNNNNNNGG 5 cut(s) 1058, 1386, 1733, 2084, 2422
BseMI GCAATG 2 cut(s) 1207, 1700
BseMII CTCAG 6 cut(s) 620, 873, 886, 1353, 1537, 1720
BseNI ACTGG 8 cut(s) 323, 352, 677, 841, 1582, 1765, 1928, 1988
BseXI GCAGC 4 cut(s) 995, 1175, 1462, 1618
BseYI CCCAGC 2 cut(s) 852, 1419
Bsh1236I CGCG 4 cut(s) 12, 22, 1994, 2163
BshFI GGCC 7 cut(s) 5, 247, 591, 1038, 1399, 2062, 2513
BshNI GGYRCC 1 cut(s) 2384
BshTI ACCGGT 1 cut(s) 45
BsiHKAI GWGCWC 2 cut(s) 1878, 2450
BsiHKCI CYCGRG 1 cut(s) 1400
BsiSI CCGG 4 cut(s) 6, 46, 72, 1683
BslFI GGGAC 2 cut(s) 2034, 2374
BslI CCNNNNNNNGG 5 cut(s) 1058, 1386, 1733, 2084, 2422
BsmAI GTCTC 2 cut(s) 287, 1156
BsmFI GGGAC 2 cut(s) 2034, 2374
BsmI GAATGC 1 cut(s) 316
BsnI GGCC 7 cut(s) 5, 247, 591, 1038, 1399, 2062, 2513
BsoBI CYCGRG 1 cut(s) 1400
Bsp119I TTCGAA 1 cut(s) 493
Bsp1286I GDGCHC 2 cut(s) 1878, 2450
Bsp143I GATC 7 cut(s) 74, 543, 717, 1534, 1812, 2164, 2369
Bsp19I CCATGG 3 cut(s) 713, 1095, 1858
BspANI GGCC 7 cut(s) 5, 247, 591, 1038, 1399, 2062, 2513
BspCNI CTCAG 6 cut(s) 619, 874, 885, 1354, 1536, 1719
BspFNI CGCG 4 cut(s) 12, 22, 1994, 2163
BspHI TCATGA 2 cut(s) 513, 1078
BspLI GGNNCC 6 cut(s) 211, 467, 1680, 2076, 2386, 2479
BspMI ACCTGC 1 cut(s) 483
BspOI GCTAGC 1 cut(s) 2343
BspPI GGATC 5 cut(s) 82, 725, 1529, 2159, 2377
BspT104I TTCGAA 1 cut(s) 493
BspT107I GGYRCC 1 cut(s) 2384
BsrDI GCAATG 2 cut(s) 1207, 1700
BsrFI RCCGGY 1 cut(s) 45
BsrI ACTGG 8 cut(s) 323, 352, 677, 841, 1582, 1765, 1928, 1988
BssAI RCCGGY 1 cut(s) 45
BssECI CCNNGG 9 cut(s) 84, 713, 1095, 1237, 1858, 1975, 2113, 2308, 2514
BssMI GATC 7 cut(s) 74, 543, 717, 1534, 1812, 2164, 2369
BssT1I CCWWGG 6 cut(s) 713, 1095, 1237, 1858, 1975, 2308
Bst2UI CCWGG 2 cut(s) 362, 1770
Bst4CI ACNGT 7 cut(s) 485, 1162, 1336, 1717, 1810, 2114, 2150
Bst6I CTCTTC 1 cut(s) 2498
BstAPI GCANNNNNTGC 2 cut(s) 686, 1484
BstBI TTCGAA 1 cut(s) 493
BstC8I GCNNGC 3 cut(s) 2305, 2341, 2511
BstDEI CTNAG 8 cut(s) 606, 813, 872, 882, 1314, 1362, 1523, 1706
BstDSI CCRYGG 5 cut(s) 713, 1095, 1858, 2113, 2514
BstEII GGTNACC 1 cut(s) 64
BstENI CCTNNNNNAGG 1 cut(s) 2420
BstF5I GGATG 7 cut(s) 94, 1372, 1417, 1537, 1944, 2089, 2412
BstFNI CGCG 4 cut(s) 12, 22, 1994, 2163
BstHHI GCGC 3 cut(s) 14, 24, 316
BstKTI GATC 7 cut(s) 77, 546, 720, 1537, 1815, 2167, 2372
BstMAI GTCTC 2 cut(s) 287, 1156
BstMBI GATC 7 cut(s) 74, 543, 717, 1534, 1812, 2164, 2369
BstMWI GCNNNNNNNGC 9 cut(s) 11, 68, 659, 686, 1197, 1484, 2059, 2313, 2519
BstNI CCWGG 2 cut(s) 362, 1770
BstNSI RCATGY 5 cut(s) 765, 952, 1616, 2027, 2307
BstPAI GACNNNNGTC 1 cut(s) 299
BstPI GGTNACC 1 cut(s) 64
BstSCI CCNGG 3 cut(s) 360, 1681, 1768
BstSFI CTRYAG 1 cut(s) 1332
BstUI CGCG 4 cut(s) 12, 22, 1994, 2163
BstV1I GCAGC 4 cut(s) 995, 1175, 1462, 1618
BstV2I GAAGAC 4 cut(s) 295, 1412, 1636, 2134
BstXI CCANNNNNNTGG 3 cut(s) 243, 1138, 1427
BsuRI GGCC 7 cut(s) 5, 247, 591, 1038, 1399, 2062, 2513
BtgI CCRYGG 5 cut(s) 713, 1095, 1858, 2113, 2514
BtsCI GGATG 7 cut(s) 94, 1372, 1417, 1537, 1944, 2089, 2412
BtsI GCAGTG 1 cut(s) 1308
BtsIMutI CAGTG 4 cut(s) 1158, 1308, 1341, 1714
BveI ACCTGC 1 cut(s) 483
Cac8I GCNNGC 3 cut(s) 2305, 2341, 2511
CciI TCATGA 2 cut(s) 513, 1078
CfoI GCGC 3 cut(s) 14, 24, 316
Cfr10I RCCGGY 1 cut(s) 45
Cfr13I GGNCC 4 cut(s) 419, 590, 1398, 1772
CseI GACGC 2 cut(s) 18, 293
CsiI ACCWGGT 2 cut(s) 360, 1768
Csp6I GTAC 4 cut(s) 231, 1504, 1766, 2385
CspAI ACCGGT 1 cut(s) 45
CspCI CAANNNNNGTGG 2 cut(s) 1147, 1182
CviQI GTAC 4 cut(s) 231, 1504, 1766, 2385
DdeI CTNAG 8 cut(s) 606, 813, 872, 882, 1314, 1362, 1523, 1706
DpnI GATC 7 cut(s) 76, 545, 719, 1536, 1814, 2166, 2371
DpnII GATC 7 cut(s) 74, 543, 717, 1534, 1812, 2164, 2369
DrdI GACNNNNNNGTC 1 cut(s) 1177
DseDI GACNNNNNNGTC 1 cut(s) 1177
EaeI YGGCCR 5 cut(s) 3, 245, 1036, 2060, 2511
Eam1104I CTCTTC 1 cut(s) 2498
EarI CTCTTC 1 cut(s) 2498
Ecl136II GAGCTC 1 cut(s) 1876
Eco130I CCWWGG 6 cut(s) 713, 1095, 1237, 1858, 1975, 2308
Eco24I GRGCYC 1 cut(s) 1878
Eco47I GGWCC 2 cut(s) 419, 1772
Eco53kI GAGCTC 1 cut(s) 1876
Eco57I CTGAAG 1 cut(s) 2376
Eco88I CYCGRG 1 cut(s) 1400
Eco91I GGTNACC 1 cut(s) 64
EcoICRI GAGCTC 1 cut(s) 1876
EcoNI CCTNNNNNAGG 1 cut(s) 2420
EcoO109I RGGNCCY 1 cut(s) 590
EcoO65I GGTNACC 1 cut(s) 64
EcoRI GAATTC 1 cut(s) 202
EcoRII CCWGG 2 cut(s) 360, 1768
EcoT14I CCWWGG 6 cut(s) 713, 1095, 1237, 1858, 1975, 2308
EcoT22I ATGCAT 1 cut(s) 2278
EcoT38I GRGCYC 1 cut(s) 1878
ErhI CCWWGG 6 cut(s) 713, 1095, 1237, 1858, 1975, 2308
FalI AAGNNNNNCTT 2 cut(s) 2126, 2158
FaqI GGGAC 2 cut(s) 2034, 2374
FauI CCCGC 1 cut(s) 751
FauNDI CATATG 2 cut(s) 424, 2278
FbaI TGATCA 1 cut(s) 1812
Fnu4HI GCNGC 8 cut(s) 15, 25, 35, 60, 984, 1189, 1476, 1632
FokI GGATG 7 cut(s) 101, 1379, 1424, 1544, 1951, 2096, 2399
FriOI GRGCYC 1 cut(s) 1878
Fsp4HI GCNGC 8 cut(s) 15, 25, 35, 60, 984, 1189, 1476, 1632
FspAI RTGCGCAY 1 cut(s) 315
FspBI CTAG 3 cut(s) 1488, 1976, 2340
FspI TGCGCA 1 cut(s) 315
GlaI GCGC 3 cut(s) 13, 23, 315
GluI GCNGC 8 cut(s) 15, 25, 35, 60, 984, 1189, 1476, 1632
GsaI CCCAGC 2 cut(s) 856, 1423
GsuI CTGGAG 2 cut(s) 1782, 2061
HaeIII GGCC 7 cut(s) 5, 247, 591, 1038, 1399, 2062, 2513
HapII CCGG 4 cut(s) 6, 46, 72, 1683
HgaI GACGC 2 cut(s) 18, 293
HhaI GCGC 3 cut(s) 14, 24, 316
Hin6I GCGC 3 cut(s) 12, 22, 314
HinP1I GCGC 3 cut(s) 12, 22, 314
HincII GTYRAC 4 cut(s) 54, 728, 1049, 1354
HindII GTYRAC 4 cut(s) 54, 728, 1049, 1354
HindIII AAGCTT 1 cut(s) 2100
HpaII CCGG 4 cut(s) 6, 46, 72, 1683
HphI GGTGA 5 cut(s) 58, 166, 1102, 1448, 2044
Hpy99I CGWCG 2 cut(s) 287, 333
HpyAV CCTTC 7 cut(s) 186, 266, 386, 1391, 1913, 2351, 2426
HpyCH4III ACNGT 7 cut(s) 485, 1162, 1336, 1717, 1810, 2114, 2150
HpyCH4IV ACGT 7 cut(s) 150, 888, 1440, 1641, 1653, 2192, 2457
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 68, 659, 686, 1197, 1484, 2059, 2313, 2519
HpyF3I CTNAG 8 cut(s) 606, 813, 872, 882, 1314, 1362, 1523, 1706
HpySE526I ACGT 7 cut(s) 150, 888, 1440, 1641, 1653, 2192, 2457
HspAI GCGC 3 cut(s) 12, 22, 314
KpnI GGTACC 1 cut(s) 2388
Ksp22I TGATCA 1 cut(s) 1812
Kzo9I GATC 7 cut(s) 74, 543, 717, 1534, 1812, 2164, 2369
LmnI GCTCC 4 cut(s) 471, 745, 1975, 2080
Lsp1109I GCAGC 4 cut(s) 995, 1175, 1462, 1618
LweI GCATC 7 cut(s) 587, 640, 727, 755, 1006, 1210, 1474
MabI ACCWGGT 2 cut(s) 360, 1768
MaeI CTAG 3 cut(s) 1488, 1976, 2340
MaeII ACGT 7 cut(s) 150, 888, 1440, 1641, 1653, 2192, 2457
MaeIII GTNAC 8 cut(s) 64, 97, 779, 797, 1142, 1436, 1580, 2105
MalI GATC 7 cut(s) 76, 545, 719, 1536, 1814, 2166, 2371
MboI GATC 7 cut(s) 74, 543, 717, 1534, 1812, 2164, 2369
MboII GAAGA 9 cut(s) 295, 401, 538, 1271, 1417, 1580, 1636, 2134, 2515
MfeI CAATTG 3 cut(s) 288, 1781, 2451
MhlI GDGCHC 2 cut(s) 1878, 2450
MlsI TGGCCA 3 cut(s) 247, 1038, 2062
MluNI TGGCCA 3 cut(s) 247, 1038, 2062
MlyI GAGTC 5 cut(s) 273, 1054, 1187, 1848, 2450
MmeI TCCRAC 6 cut(s) 97, 1699, 1768, 1831, 2236, 2413
Mox20I TGGCCA 3 cut(s) 247, 1038, 2062
Mph1103I ATGCAT 1 cut(s) 2278
MroXI GAANNNNTTC 3 cut(s) 324, 530, 1380
MscI TGGCCA 3 cut(s) 247, 1038, 2062
MseI TTAA 3 cut(s) 669, 789, 2376
MslI CAYNNNNRTG 2 cut(s) 241, 1596
Msp20I TGGCCA 3 cut(s) 247, 1038, 2062
MspI CCGG 4 cut(s) 6, 46, 72, 1683
MspR9I CCNGG 3 cut(s) 362, 1683, 1770
MunI CAATTG 3 cut(s) 288, 1781, 2451
Mva1269I GAATGC 1 cut(s) 316
MvaI CCWGG 2 cut(s) 362, 1770
MvnI CGCG 4 cut(s) 12, 22, 1994, 2163
MwoI GCNNNNNNNGC 9 cut(s) 11, 68, 659, 686, 1197, 1484, 2059, 2313, 2519
NciI CCSGG 1 cut(s) 1683
NcoI CCATGG 3 cut(s) 713, 1095, 1858
NdeI CATATG 2 cut(s) 424, 2278
NdeII GATC 7 cut(s) 74, 543, 717, 1534, 1812, 2164, 2369
NheI GCTAGC 1 cut(s) 2339
NlaIV GGNNCC 6 cut(s) 211, 467, 1680, 2076, 2386, 2479
NmuCI GTSAC 5 cut(s) 64, 97, 779, 1436, 2105
NsbI TGCGCA 1 cut(s) 315
NsiI ATGCAT 1 cut(s) 2278
NspI RCATGY 5 cut(s) 765, 952, 1616, 2027, 2307
NspV TTCGAA 1 cut(s) 493
OliI CACNNNNGTG 1 cut(s) 241
PaeI GCATGC 1 cut(s) 2307
PagI TCATGA 2 cut(s) 513, 1078
PciI ACATGT 3 cut(s) 761, 948, 1612
PctI GAATGC 1 cut(s) 316
PdmI GAANNNNTTC 3 cut(s) 324, 530, 1380
PfeI GAWTC 5 cut(s) 382, 490, 635, 692, 1376
PflMI CCANNNNNTGG 1 cut(s) 2084
PfoI TCCNGGA 1 cut(s) 1681
PinAI ACCGGT 1 cut(s) 45
PkrI GCNGC 8 cut(s) 16, 26, 36, 61, 985, 1190, 1477, 1633
PleI GAGTC 5 cut(s) 273, 1053, 1186, 1848, 2449
PpsI GAGTC 5 cut(s) 273, 1053, 1186, 1848, 2449
PscI ACATGT 3 cut(s) 761, 948, 1612
PshAI GACNNNNGTC 1 cut(s) 299
PsiI TTATAA 1 cut(s) 1638
Psp124BI GAGCTC 1 cut(s) 1878
Psp6I CCWGG 2 cut(s) 360, 1768
PspEI GGTNACC 1 cut(s) 64
PspFI CCCAGC 2 cut(s) 852, 1419
PspGI CCWGG 2 cut(s) 360, 1768
PspN4I GGNNCC 6 cut(s) 211, 467, 1680, 2076, 2386, 2479
PspPI GGNCC 4 cut(s) 419, 590, 1398, 1772
RsaI GTAC 4 cut(s) 232, 1505, 1767, 2386
RsaNI GTAC 4 cut(s) 231, 1504, 1766, 2385
RseI CAYNNNNRTG 2 cut(s) 241, 1596
SacI GAGCTC 1 cut(s) 1878
SaqAI TTAA 3 cut(s) 669, 789, 2376
SatI GCNGC 8 cut(s) 15, 25, 35, 60, 984, 1189, 1476, 1632
Sau3AI GATC 7 cut(s) 74, 543, 717, 1534, 1812, 2164, 2369
Sau96I GGNCC 4 cut(s) 419, 590, 1398, 1772
SchI GAGTC 5 cut(s) 273, 1054, 1187, 1848, 2450
ScrFI CCNGG 3 cut(s) 362, 1683, 1770
SduI GDGCHC 2 cut(s) 1878, 2450
SexAI ACCWGGT 2 cut(s) 360, 1768
SfaNI GCATC 7 cut(s) 587, 640, 727, 755, 1006, 1210, 1474
SfcI CTRYAG 1 cut(s) 1332
SfuI TTCGAA 1 cut(s) 493
SinI GGWCC 2 cut(s) 419, 1772
SmiMI CAYNNNNRTG 2 cut(s) 241, 1596
SmlI CTYRAG 1 cut(s) 582
SmoI CTYRAG 1 cut(s) 582
SphI GCATGC 1 cut(s) 2307
SspI AATATT 1 cut(s) 1123
SspMI CTAG 3 cut(s) 1488, 1976, 2340
SstI GAGCTC 1 cut(s) 1878
StyD4I CCNGG 3 cut(s) 360, 1681, 1768
StyI CCWWGG 6 cut(s) 713, 1095, 1237, 1858, 1975, 2308
TaaI ACNGT 7 cut(s) 485, 1162, 1336, 1717, 1810, 2114, 2150
TaiI ACGT 7 cut(s) 153, 891, 1443, 1644, 1656, 2195, 2460
TaqI TCGA 6 cut(s) 226, 282, 493, 1154, 1181, 1659
TatI WGTACW 1 cut(s) 230
TauI GCSGC 4 cut(s) 17, 27, 37, 62
TfiI GAWTC 5 cut(s) 382, 490, 635, 692, 1376
Tru1I TTAA 3 cut(s) 669, 789, 2376
Tru9I TTAA 3 cut(s) 669, 789, 2376
TscAI CASTG 4 cut(s) 1165, 1315, 1341, 1714
TseFI GTSAC 5 cut(s) 64, 97, 779, 1436, 2105
TseI GCWGC 4 cut(s) 983, 1188, 1475, 1631
Tsp45I GTSAC 5 cut(s) 64, 97, 779, 1436, 2105
TspGWI ACGGA 1 cut(s) 1424
TspRI CASTG 4 cut(s) 1165, 1315, 1341, 1714
Van91I CCANNNNNTGG 1 cut(s) 2084
VpaK11BI GGWCC 2 cut(s) 419, 1772
XagI CCTNNNNNAGG 1 cut(s) 2420
XapI RAATTY 5 cut(s) 202, 529, 2210, 2236, 2333
XceI RCATGY 5 cut(s) 765, 952, 1616, 2027, 2307
XcmI CCANNNNNNNNNTGG 1 cut(s) 1135
XmaJI CCTAGG 1 cut(s) 1975
XmnI GAANNNNTTC 3 cut(s) 324, 530, 1380
XspI CTAG 3 cut(s) 1488, 1976, 2340
Zsp2I ATGCAT 1 cut(s) 2278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.