Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0001959

Physical Location & Seq

Genomic Coordinates Reverse (-)

7881180 .. 7888963

Gene Structure 7784 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016583

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1872 bp

ATGTCAAAGGAAGGTGGACTCGATGCAATTGAGACTTATATCTTCTGGAATGCTCATGAACCAGTTTGTCGTGAATACTATTTTATTGGTAATCTTGACCTGGTTAGGTTTGTCAAAACGATTCAACATGAAGGACTGTATGCTGTTCTTCAGATTGGTCCATATCTTTGTGCTGAATGGAATTATGGGTTAAATGAGATGAAGAATTTCACTACATTGATGGTGGATATGATGAAAAAAGAGAAACTATTTGCATGTCAAGGAGGCCCTATCATTATTGCTCCAGGTACATATGCTTATATATATCATTGTACTGGCTCATGGTTCATATCGATCTATCTTCTATTGAAAATGAATGGGAATGTGGAATCATACTACGGAGATGCTGGAAAAGCATACATTAATTGGTCTGCAAATTTTGCTGAATCACTGAACATCGGAGTTCCATGGATTATAAACACATGTAATGGCTGGTATTGTGACACATTCAAGCCAAGTAATCCTAGGCCTAACACTCCAAAGATGTGGACTGAAAATTGGACTGGCTGGACGTTTGCAGTAGCAAGGTTTTTCCAATTTGGAGGCACATTCCAAAACTATTACATGATAATTAGTACATTATATCATGGTGGAACAAACTTTGGTAGAACAGCAGCTTCATACATGACCACCTTGTATGATTATGATGCCCCTCTTGATGAGTATGGCCATTTGAATCAACCAAAATGGGGGCATTTGAAAGAGCTTCATGAGGTTTTGAAATCCATGGAATACACTTTCACCTATGGCAATATTACCACCACAGAATTGGTTAACTCGATTTTAGCTGCATTTTTGGCAATGCAAATAACAGTTCAGATACTACGTACAATCACTTTCCAAGGAAACACATACACCATTCCTGCTTGGTCTGTTAATATTCTTCCTGATTGCGAAAAAGAAGCATACAAAAATGTAAACACCCAAACTACAGTGAAAGTAAAAAGTGTTAACAAGGCTGTGGATGAACCTGAATCCCTTCATTGGTCATGGAGGCCCGAGAAGACGGATGACTCCCAGCTTCTTGGAAAAGGTGAAGTTGTGGAAAATCATCTTATGGAACAAAAAGATGCTGCAAATGATGCTAGTGATTATCTTTGGCACATGACAAAGGATGATCCAATTTTGCGTGGGAACATGACACTGAGGATAAATGAAACTGTTTACATTCTTCATGCTTATGTTAATGGAGAACATGTTGGTTCTCAATGGGCTGCTTATAACGTGTTCAACTACGTTTTCGAGAAACCTATCAGGTTGGTTCCCGGAGTGAACACAATTTCATTGCTCAGTGCTACTGTTGGATTTCCGAATTATGGAGGTGGATTTGAGGAAAACAAAACTGGAGTACCTGGTCCGGCCGGTTCAATCGAACACAAATGGTCATACAAGGTTGTACTCCATGGCTTGAAAGATGAGCTCTTTACTTCTGCTTCTCGTTTTGCAACTAAATGGTCAGTTGAAGGATTACCTGTACGTAAACAGGATGATGACATGGGCCATGCTTGGGTGAACGGGCATAGCATTGGGCGATATTGGCCAAGCTACTTGGCTCCAGAGGATGGTTGTAGTGTTGAAGCTTGTGACTACCGTGGTGCATACGACAATAACAAAGATGATGATGACAACACATTGATCTTGTTCGAGGAATTTGGTGACAACCCATCTTATGTGAATTTTCAAATGATCAGAGTTGGAACGATTTGTGCAAATGCATATAAGGTTGGGTTCGGGTCGGCCAGGCTCGGTTTTGAGCCGAACCCGACTTCGACCGGGAATTTGTGGTTGGGCCTAATTTTGGACCGACAATTGTTTCTAAATGGGTTGGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BetaGal_dom4_5 Gal_Lectin Glyco_hydro_35

Protein Analysis

624

Amino Acids

70.6

Weight (kDa)

5.32

Isoelectric Point (pI)

29.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	2 - 63	3.3e-22	Glycosyl hydrolases family 35
Glyco_hydro_35	PF01301	65 - 251	7.1e-21	Glycosyl hydrolases family 35
GHD	PF17834	290 - 317	2.3e-08	Beta-sandwich domain in beta galactosidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001959)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31740 AT2G04060 AT5G20710 AT5G20710
fragaria_vesca	FvH4_1g09120 FvH4_1g09190 FvH4_1g09190 FvH4_1g26380
malus_domestica	MD02G1096400.v1.1 MD03G1276000.v1.1 MD15G1220900.v1.1 MD15G1221000.v1.1
prunus_persica	Prupe.7G194500_v2.0.a1
pyrus_communis	pycom02g07640
rosa_chinensis	RchiOBHm_Chr2g0095511 RchiOBHm_Chr2g0095591 RchiOBHm_Chr5g0030701
rosa_laevigata	RLG00000016574 RLG00000016582 RLG00000016583
rosa_multiflora	Rmu_co8462209.1_g000001 Rmu_sc0003238.1_g000025 Rmu_sc0003238.1_g000028 Rmu_sc0004719.1_g000009
rosa_roxburghii	Rroxscaffold_2G00146240
rosa_rugosa	Rorug02G0051800 Rorug02G0052600 Rorug05G0120200
rosa_samantha	Rh2AG099800 Rh2AG100400 Rh2BG101100 Rh2BG102200 Rh2CG103300 Rh2CG104600 Rh2DG100700 Rh2DG101500 Rh5AG211800 Rh5BG210700 Rh5CG234400 Rh5CG234500 Rh5DG216100
rosa_wichuraiana	Rw2G007570 Rw2G007630 Rw2G007640 Rw5G019420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	455, 1260
AccB7I	CCANNNNNTGG	1 cut(s)	1601
AclWI	GGATC	1 cut(s)	1151
AcoI	YGGCCR	4 cut(s)	706, 1398, 1577, 1776
AcsI	RAATTY	5 cut(s)	205, 415, 1688, 1714, 1816
AcuI	CTGAAG	1 cut(s)	134
AfaI	GTAC	7 cut(s)	289, 313, 616, 868, 1389, 1437, 1515
AfiI	CCNNNNNNNGG	6 cut(s)	728, 1023, 1355, 1546, 1601, 1837
AflIII	ACRYGT	3 cut(s)	461, 1234, 1263
AjnI	CCWGG	4 cut(s)	99, 283, 1390, 1778
AjuI	GAANNNNNNNTTGG	2 cut(s)	1808, 1840
AluBI	AGCT	7 cut(s)	656, 745, 827, 1060, 1459, 1584, 1619
AluI	AGCT	7 cut(s)	656, 745, 827, 1060, 1459, 1584, 1619
Alw21I	GWGCWC	1 cut(s)	1461
Alw26I	GTCTC	1 cut(s)	26
AlwI	GGATC	1 cut(s)	1151
Ama87I	CYCGRG	1 cut(s)	1037
AoxI	GGCC	9 cut(s)	265, 506, 706, 1034, 1398, 1537, 1577, 1776, 1828
ApeKI	GCWGC	4 cut(s)	653, 827, 1112, 1253
ApoI	RAATTY	5 cut(s)	205, 415, 1688, 1714, 1816
ArsI	GACNNNNNNTTYG	2 cut(s)	1801, 1833
AseI	ATTAAT	1 cut(s)	402
Asp700I	GAANNNNTTC	2 cut(s)	206, 1017
AspA2I	CCTAGG	1 cut(s)	503
AspS9I	GGNCC	7 cut(s)	158, 266, 1035, 1394, 1537, 1828, 1840
AsuC2I	CCSGG	2 cut(s)	1305, 1813
AsuHPI	GGTGA	4 cut(s)	772, 1085, 1561, 1706
AvaI	CYCGRG	1 cut(s)	1037
AvaII	GGWCC	3 cut(s)	158, 1394, 1840
AvrII	CCTAGG	1 cut(s)	503
BaeI	ACNNNNGTAYC	2 cut(s)	1371, 1404
BalI	TGGCCA	2 cut(s)	708, 1579
BanII	GRGCYC	1 cut(s)	1461
BbsI	GAAGAC	1 cut(s)	1049
Bbv12I	GWGCWC	1 cut(s)	1461
BbvI	GCAGC	4 cut(s)	665, 814, 1099, 1240
BccI	CCATC	3 cut(s)	214, 1595, 1711
BciT130I	CCWGG	4 cut(s)	101, 285, 1392, 1780
BclI	TGATCA	1 cut(s)	1725
BcnI	CCSGG	2 cut(s)	1305, 1813
BcoDI	GTCTC	1 cut(s)	26
BfaI	CTAG	2 cut(s)	504, 1125
BfmI	CTRYAG	1 cut(s)	969
BisI	GCNGC	4 cut(s)	654, 828, 1113, 1254
BlnI	CCTAGG	1 cut(s)	503
BlsI	GCNGC	4 cut(s)	655, 829, 1114, 1255
Bme1390I	CCNGG	6 cut(s)	101, 285, 1305, 1392, 1780, 1813
Bme18I	GGWCC	3 cut(s)	158, 1394, 1840
BmeT110I	CYCGRG	1 cut(s)	1037
BmgT120I	GGNCC	7 cut(s)	158, 266, 1035, 1394, 1537, 1828, 1840
BmiI	GGNNCC	2 cut(s)	1302, 1593
BmrFI	CCNGG	6 cut(s)	101, 285, 1305, 1392, 1780, 1813
BmsI	GCATC	5 cut(s)	13, 373, 676, 1099, 1111
BpiI	GAAGAC	1 cut(s)	1049
BpmI	CTGGAG	3 cut(s)	267, 1404, 1578
BpuMI	CCSGG	2 cut(s)	1305, 1813
Bsa29I	ATCGAT	1 cut(s)	332
BsaAI	YACGTR	2 cut(s)	866, 1517
BsaJI	CCNNGG	6 cut(s)	446, 503, 765, 880, 1441, 1630
BsaXI	ACNNNNNCTCC	2 cut(s)	1221, 1251
Bsc4I	CCNNNNNNNGG	6 cut(s)	728, 1023, 1355, 1546, 1601, 1837
Bse118I	RCCGGY	1 cut(s)	1400
Bse1I	ACTGG	4 cut(s)	62, 319, 547, 1387
Bse3DI	GCAATG	2 cut(s)	846, 1322
BseBI	CCWGG	4 cut(s)	101, 285, 1392, 1780
BseCI	ATCGAT	1 cut(s)	332
BseDI	CCNNGG	6 cut(s)	446, 503, 765, 880, 1441, 1630
BseGI	GGATG	5 cut(s)	1009, 1054, 1159, 1531, 1606
BseLI	CCNNNNNNNGG	6 cut(s)	728, 1023, 1355, 1546, 1601, 1837
BseMI	GCAATG	2 cut(s)	846, 1322
BseMII	CTCAG	2 cut(s)	1175, 1342
BseNI	ACTGG	4 cut(s)	62, 319, 547, 1387
BseX3I	CGGCCG	1 cut(s)	1398
BseXI	GCAGC	4 cut(s)	665, 814, 1099, 1240
BseYI	CCCAGC	1 cut(s)	1056
Bsh1285I	CGRYCG	2 cut(s)	1401, 1812
BshFI	GGCC	9 cut(s)	267, 508, 708, 1036, 1400, 1539, 1579, 1778, 1830
BshVI	ATCGAT	1 cut(s)	332
BsiEI	CGRYCG	2 cut(s)	1401, 1812
BsiHKAI	GWGCWC	1 cut(s)	1461
BsiHKCI	CYCGRG	1 cut(s)	1037
BsiSI	CCGG	4 cut(s)	1305, 1397, 1401, 1812
BslI	CCNNNNNNNGG	6 cut(s)	728, 1023, 1355, 1546, 1601, 1837
BsmAI	GTCTC	1 cut(s)	26
BsmI	GAATGC	1 cut(s)	55
BsnI	GGCC	9 cut(s)	267, 508, 708, 1036, 1400, 1539, 1579, 1778, 1830
BsoBI	CYCGRG	1 cut(s)	1037
Bsp1286I	GDGCHC	1 cut(s)	1461
Bsp143I	GATC	4 cut(s)	333, 1156, 1674, 1725
Bsp19I	CCATGG	3 cut(s)	446, 765, 1441
BspANI	GGCC	9 cut(s)	267, 508, 708, 1036, 1400, 1539, 1579, 1778, 1830
BspCNI	CTCAG	2 cut(s)	1176, 1341
BspDI	ATCGAT	1 cut(s)	332
BspHI	TCATGA	2 cut(s)	55, 748
BspLI	GGNNCC	2 cut(s)	1302, 1593
BspPI	GGATC	1 cut(s)	1151
BsrDI	GCAATG	2 cut(s)	846, 1322
BsrFI	RCCGGY	1 cut(s)	1400
BsrI	ACTGG	4 cut(s)	62, 319, 547, 1387
BssAI	RCCGGY	1 cut(s)	1400
BssECI	CCNNGG	6 cut(s)	446, 503, 765, 880, 1441, 1630
BssMI	GATC	4 cut(s)	333, 1156, 1674, 1725
BssT1I	CCWWGG	5 cut(s)	446, 503, 765, 880, 1441
Bst2UI	CCWGG	4 cut(s)	101, 285, 1392, 1780
Bst4CI	ACNGT	6 cut(s)	138, 853, 973, 1201, 1339, 1631
BstAPI	GCANNNNNTGC	2 cut(s)	419, 1121
BstBAI	YACGTR	2 cut(s)	866, 1517
BstDEI	CTNAG	2 cut(s)	1184, 1328
BstDSI	CCRYGG	4 cut(s)	446, 765, 1441, 1630
BstF5I	GGATG	5 cut(s)	1009, 1054, 1159, 1531, 1606
BstKTI	GATC	4 cut(s)	336, 1159, 1677, 1728
BstMAI	GTCTC	1 cut(s)	26
BstMBI	GATC	4 cut(s)	333, 1156, 1674, 1725
BstMCI	CGRYCG	2 cut(s)	1401, 1812
BstMWI	GCNNNNNNNGC	5 cut(s)	392, 419, 836, 1121, 1576
BstNI	CCWGG	4 cut(s)	101, 285, 1392, 1780
BstNSI	RCATGY	3 cut(s)	258, 465, 1238
BstSCI	CCNGG	6 cut(s)	99, 283, 1303, 1390, 1778, 1811
BstSFI	CTRYAG	1 cut(s)	969
BstSNI	TACGTA	2 cut(s)	866, 1517
BstV1I	GCAGC	4 cut(s)	665, 814, 1099, 1240
BstV2I	GAAGAC	1 cut(s)	1049
BstXI	CCANNNNNNTGG	3 cut(s)	525, 808, 1064
BstZI	CGGCCG	1 cut(s)	1398
Bsu15I	ATCGAT	1 cut(s)	332
BsuRI	GGCC	9 cut(s)	267, 508, 708, 1036, 1400, 1539, 1579, 1778, 1830
BsuTUI	ATCGAT	1 cut(s)	332
BtgI	CCRYGG	4 cut(s)	446, 765, 1441, 1630
BtsCI	GGATG	5 cut(s)	1009, 1054, 1159, 1531, 1606
BtsIMutI	CAGTG	4 cut(s)	428, 978, 1181, 1336
CciI	TCATGA	2 cut(s)	55, 748
Cfr10I	RCCGGY	1 cut(s)	1400
Cfr13I	GGNCC	7 cut(s)	158, 266, 1035, 1394, 1537, 1828, 1840
ClaI	ATCGAT	1 cut(s)	332
CsiI	ACCWGGT	2 cut(s)	99, 1390
Csp6I	GTAC	7 cut(s)	288, 312, 615, 867, 1388, 1436, 1514
CviQI	GTAC	7 cut(s)	288, 312, 615, 867, 1388, 1436, 1514
DdeI	CTNAG	2 cut(s)	1184, 1328
DpnI	GATC	4 cut(s)	335, 1158, 1676, 1727
DpnII	GATC	4 cut(s)	333, 1156, 1674, 1725
EaeI	YGGCCR	4 cut(s)	706, 1398, 1577, 1776
EagI	CGGCCG	1 cut(s)	1398
Ecl136II	GAGCTC	1 cut(s)	1459
EclXI	CGGCCG	1 cut(s)	1398
Eco105I	TACGTA	2 cut(s)	866, 1517
Eco130I	CCWWGG	5 cut(s)	446, 503, 765, 880, 1441
Eco147I	AGGCCT	1 cut(s)	508
Eco24I	GRGCYC	1 cut(s)	1461
Eco47I	GGWCC	3 cut(s)	158, 1394, 1840
Eco52I	CGGCCG	1 cut(s)	1398
Eco53kI	GAGCTC	1 cut(s)	1459
Eco57I	CTGAAG	1 cut(s)	134
Eco88I	CYCGRG	1 cut(s)	1037
EcoICRI	GAGCTC	1 cut(s)	1459
EcoO109I	RGGNCCY	1 cut(s)	266
EcoRII	CCWGG	4 cut(s)	99, 283, 1390, 1778
EcoT14I	CCWWGG	5 cut(s)	446, 503, 765, 880, 1441
EcoT22I	ATGCAT	1 cut(s)	1756
EcoT38I	GRGCYC	1 cut(s)	1461
ErhI	CCWWGG	5 cut(s)	446, 503, 765, 880, 1441
FauNDI	CATATG	1 cut(s)	292
FbaI	TGATCA	1 cut(s)	1725
Fnu4HI	GCNGC	4 cut(s)	654, 828, 1113, 1254
FokI	GGATG	5 cut(s)	1016, 1061, 1166, 1538, 1613
FriOI	GRGCYC	1 cut(s)	1461
Fsp4HI	GCNGC	4 cut(s)	654, 828, 1113, 1254
FspBI	CTAG	2 cut(s)	504, 1125
GluI	GCNGC	4 cut(s)	654, 828, 1113, 1254
GsaI	CCCAGC	1 cut(s)	1060
GsuI	CTGGAG	3 cut(s)	267, 1404, 1578
HaeIII	GGCC	9 cut(s)	267, 508, 708, 1036, 1400, 1539, 1579, 1778, 1830
HapII	CCGG	4 cut(s)	1305, 1397, 1401, 1812
HincII	GTYRAC	2 cut(s)	814, 991
HindII	GTYRAC	2 cut(s)	814, 991
HindIII	AAGCTT	1 cut(s)	1617
HinfI	GANTC	7 cut(s)	18, 121, 368, 425, 715, 1013, 1052
HpaI	GTTAAC	2 cut(s)	814, 991
HpaII	CCGG	4 cut(s)	1305, 1397, 1401, 1812
HphI	GGTGA	4 cut(s)	772, 1085, 1561, 1706
Hpy166II	GTNNAC	9 cut(s)	17, 528, 814, 958, 991, 1204, 1312, 1520, 1552
Hpy188I	TCNGA	5 cut(s)	153, 440, 858, 1350, 1730
Hpy188III	TCNNGA	9 cut(s)	46, 56, 71, 95, 695, 749, 926, 1282, 1595
Hpy8I	GTNNAC	9 cut(s)	17, 528, 814, 958, 991, 1204, 1312, 1520, 1552
HpyAV	CCTTC	4 cut(s)	5, 125, 1028, 1496
HpyCH4III	ACNGT	6 cut(s)	138, 853, 973, 1201, 1339, 1631
HpyCH4IV	ACGT	5 cut(s)	551, 865, 1263, 1275, 1516
HpyF10VI	GCNNNNNNNGC	5 cut(s)	392, 419, 836, 1121, 1576
HpyF3I	CTNAG	2 cut(s)	1184, 1328
HpySE526I	ACGT	5 cut(s)	551, 865, 1263, 1275, 1516
Ksp22I	TGATCA	1 cut(s)	1725
KspAI	GTTAAC	2 cut(s)	814, 991
Kzo9I	GATC	4 cut(s)	333, 1156, 1674, 1725
LmnI	GCTCC	2 cut(s)	286, 1597
Lsp1109I	GCAGC	4 cut(s)	665, 814, 1099, 1240
LweI	GCATC	5 cut(s)	13, 373, 676, 1099, 1111
MabI	ACCWGGT	2 cut(s)	99, 1390
MaeI	CTAG	2 cut(s)	504, 1125
MaeII	ACGT	5 cut(s)	551, 865, 1263, 1275, 1516
MaeIII	GTNAC	3 cut(s)	479, 1622, 1694
MalI	GATC	4 cut(s)	335, 1158, 1676, 1727
MboI	GATC	4 cut(s)	333, 1156, 1674, 1725
MboII	GAAGA	7 cut(s)	34, 140, 214, 332, 914, 1054, 1202
MfeI	CAATTG	2 cut(s)	27, 1847
MhlI	GDGCHC	1 cut(s)	1461
MlsI	TGGCCA	2 cut(s)	708, 1579
MluNI	TGGCCA	2 cut(s)	708, 1579
MlyI	GAGTC	2 cut(s)	12, 1046
MmeI	TCCRAC	2 cut(s)	1321, 1714
Mox20I	TGGCCA	2 cut(s)	708, 1579
Mph1103I	ATGCAT	1 cut(s)	1756
MroXI	GAANNNNTTC	2 cut(s)	206, 1017
MscI	TGGCCA	2 cut(s)	708, 1579
MseI	TTAA	6 cut(s)	191, 402, 813, 915, 990, 1224
MslI	CAYNNNNRTG	1 cut(s)	1218
Msp20I	TGGCCA	2 cut(s)	708, 1579
MspI	CCGG	4 cut(s)	1305, 1397, 1401, 1812
MspR9I	CCNGG	6 cut(s)	101, 285, 1305, 1392, 1780, 1813
MunI	CAATTG	2 cut(s)	27, 1847
Mva1269I	GAATGC	1 cut(s)	55
MvaI	CCWGG	4 cut(s)	101, 285, 1392, 1780
MwoI	GCNNNNNNNGC	5 cut(s)	392, 419, 836, 1121, 1576
NciI	CCSGG	2 cut(s)	1305, 1813
NcoI	CCATGG	3 cut(s)	446, 765, 1441
NdeI	CATATG	1 cut(s)	292
NdeII	GATC	4 cut(s)	333, 1156, 1674, 1725
NlaIV	GGNNCC	2 cut(s)	1302, 1593
NmuCI	GTSAC	3 cut(s)	479, 1622, 1694
NsiI	ATGCAT	1 cut(s)	1756
NspI	RCATGY	3 cut(s)	258, 465, 1238
PagI	TCATGA	2 cut(s)	55, 748
PceI	AGGCCT	1 cut(s)	508
PciI	ACATGT	2 cut(s)	461, 1234
PctI	GAATGC	1 cut(s)	55
PdmI	GAANNNNTTC	2 cut(s)	206, 1017
PfeI	GAWTC	5 cut(s)	121, 368, 425, 715, 1013
PflMI	CCANNNNNTGG	1 cut(s)	1601
PfoI	TCCNGGA	1 cut(s)	1303
PkrI	GCNGC	4 cut(s)	655, 829, 1114, 1255
PleI	GAGTC	2 cut(s)	12, 1046
PpsI	GAGTC	2 cut(s)	12, 1046
Ppu21I	YACGTR	2 cut(s)	866, 1517
PscI	ACATGT	2 cut(s)	461, 1234
PshBI	ATTAAT	1 cut(s)	402
PsiI	TTATAA	2 cut(s)	455, 1260
Psp124BI	GAGCTC	1 cut(s)	1461
Psp6I	CCWGG	4 cut(s)	99, 283, 1390, 1778
PspFI	CCCAGC	1 cut(s)	1056
PspGI	CCWGG	4 cut(s)	99, 283, 1390, 1778
PspN4I	GGNNCC	2 cut(s)	1302, 1593
PspPI	GGNCC	7 cut(s)	158, 266, 1035, 1394, 1537, 1828, 1840
RsaI	GTAC	7 cut(s)	289, 313, 616, 868, 1389, 1437, 1515
RsaNI	GTAC	7 cut(s)	288, 312, 615, 867, 1388, 1436, 1514
RseI	CAYNNNNRTG	1 cut(s)	1218
SacI	GAGCTC	1 cut(s)	1461
SaqAI	TTAA	6 cut(s)	191, 402, 813, 915, 990, 1224
SatI	GCNGC	4 cut(s)	654, 828, 1113, 1254
Sau3AI	GATC	4 cut(s)	333, 1156, 1674, 1725
Sau96I	GGNCC	7 cut(s)	158, 266, 1035, 1394, 1537, 1828, 1840
SchI	GAGTC	2 cut(s)	12, 1046
ScrFI	CCNGG	6 cut(s)	101, 285, 1305, 1392, 1780, 1813
SduI	GDGCHC	1 cut(s)	1461
SexAI	ACCWGGT	2 cut(s)	99, 1390
SfaNI	GCATC	5 cut(s)	13, 373, 676, 1099, 1111
SfcI	CTRYAG	1 cut(s)	969
SinI	GGWCC	3 cut(s)	158, 1394, 1840
SmiMI	CAYNNNNRTG	1 cut(s)	1218
SnaBI	TACGTA	2 cut(s)	866, 1517
SseBI	AGGCCT	1 cut(s)	508
SspI	AATATT	2 cut(s)	793, 919
SspMI	CTAG	2 cut(s)	504, 1125
SstI	GAGCTC	1 cut(s)	1461
StuI	AGGCCT	1 cut(s)	508
StyD4I	CCNGG	6 cut(s)	99, 283, 1303, 1390, 1778, 1811
StyI	CCWWGG	5 cut(s)	446, 503, 765, 880, 1441
TaaI	ACNGT	6 cut(s)	138, 853, 973, 1201, 1339, 1631
TaiI	ACGT	5 cut(s)	554, 868, 1266, 1278, 1519
TaqI	TCGA	7 cut(s)	21, 332, 818, 1281, 1410, 1683, 1808
TaqII	GACCGA	1 cut(s)	1857
TatI	WGTACW	3 cut(s)	311, 614, 1435
TfiI	GAWTC	5 cut(s)	121, 368, 425, 715, 1013
Tru1I	TTAA	6 cut(s)	191, 402, 813, 915, 990, 1224
Tru9I	TTAA	6 cut(s)	191, 402, 813, 915, 990, 1224
TscAI	CASTG	4 cut(s)	435, 978, 1188, 1336
TseFI	GTSAC	3 cut(s)	479, 1622, 1694
TseI	GCWGC	4 cut(s)	653, 827, 1112, 1253
Tsp45I	GTSAC	3 cut(s)	479, 1622, 1694
TspGWI	ACGGA	2 cut(s)	393, 1061
TspRI	CASTG	4 cut(s)	435, 978, 1188, 1336
Van91I	CCANNNNNTGG	1 cut(s)	1601
VpaK11BI	GGWCC	3 cut(s)	158, 1394, 1840
VspI	ATTAAT	1 cut(s)	402
XapI	RAATTY	5 cut(s)	205, 415, 1688, 1714, 1816
XceI	RCATGY	3 cut(s)	258, 465, 1238
XcmI	CCANNNNNNNNNTGG	1 cut(s)	805
XmaJI	CCTAGG	1 cut(s)	503
XmnI	GAANNNNTTC	2 cut(s)	206, 1017
XspI	CTAG	2 cut(s)	504, 1125
Zsp2I	ATGCAT	1 cut(s)	1756

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.