Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0001959

Physical Location & Seq

Genomic Coordinates Forward (+)

8086560 .. 8090438

Gene Structure 3879 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG100700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1962 bp

ATGGCACTCCCCAAGAGTTTTGCGTTTTCTCTTTGTTTCTGTCTTTCTGTGATCCTCTGCAGCCTCGTTGCTAATGCTACTGACGTTTCTTATGATGGCAGAGCCATCACCATTGATGGCAAACGAAGGATTTTGAATTCTGGTTCCATACATTATCCTCGAAGTACACCACAAATGTGGCCAGACTTGATTAGGAAGTCAAAGGAAGGTGGACTCGATGCAATTGAGACTTATGTCTTCTGGAATGCGCATGAACCAGTTCGTCGTGAATACGATTTTACTGGTAATCTTGACCTGGTTAGGTTTATCAAAACGATTCAAGATGAAGGACTTTATGCTGTTCTTCGGATTGGTCCATATTATCATGGTGGAACAAACTTTGGTAGAACAGCAGCTTCATACATTACCACCTCGTATGATTATGATGCCCCTCTTGATGAGTATGGCCATCTGAGTCAACCAAAATGGGGGCATTTGAAAGAACTTCATGAGGTTTTGAAATCCATGGAATATACTCTCACCTATGGCAATATTACCACCACAGAATTGGGTAACTCTGTCTCAACCACTGTGTTTGCGACAAATGAGTCAAGTAGCTGCATTTTTGGCAATGCAAATAGCAGTTCAGATGCTACAATCACTTTCCAAGGAAACACATACACCATTCCTGCTTGGTCTGTTAGTATTCTTCCTGATTGCAAAAAAGAAGCATACAACACTGCTAAGGTAAACACCCAAACTACAGTGAAAGTAAAAAGTGTCAACAAGGCTGAGGATGAACCTGAATCCCTTCATTGGTCATGGAGGCCCGAGAAGACGGATGACACCCAGCTTCTTGGAAAAGGTGAAGTTGTGGCAAGTCGTCTTATGGAACAAAAAGAGGCTGCAAATGATGCTAGTGATTATCTTTGGTACATGACAAGTGTTCAACTCAGTAAGGATGATCCAATTTTGAGTGGGAACATGACATTGAGGATAAATGAAACTGGTTACATTCTTCATGCTTATGTTAATGGAGAACATGTTGGTTCTCAATGGGCTGCTTATAACGTCTTCAACTACGTTTTCGAGAAACCTATCAAGTTGGTTCCCGGAGTGAACACAATTTCATTGCTCAGTGCTACTGTTGGATTTCCCAATTATGGAGGTGGATTTGAGGACATCAAAACTGGAGTAGCTGGTCCTGTTCAATTGGTTGGACAAAGTGGAGATGAAACTGTGATCAAGGATTTGTCACAACACAAATGGTCATACAAGGTTGGACTCCATGGCTTGAAAGACGAGCTCTTTAGCTCTGCTTCTCGTTTTGCAAATAAATGGTCAGTTGAAGGTTTACCAGTAAACAGAACGATGACATGGTATAAGACAACTTTCAAAGCTCCCCTAGGAACTGAACCAGTCGTCGTGGACTTACACGGGTTGGGTAAGGGACATGCTTGGGTGAACGGGCATAGCATTGGGCGATATTGGCCAAGCTACTTGGCTCCAGAGGATGGTTGTAGTGTTGAAGCTTGTGACTACCGTGGTGCATACGACAATAACAAGTGTGTCTTCAACTGTGGCAAACCTACTCAAAGATGGTATCATGTTCCGCGATCCTTTTTGCAAGATGATGAGAACACATTGGTCTTGTTTGAGGAATTTGGTGGCAACCCATCTTATGTGAATTTCCAGACAATCAGAGTTGGAACTATTTGTGCAAATGCATATGAGAATCATACATTGGAATTGGCATGCCAAGGTCGCCCCATTTCAGCAATCAAATTTGCTAGCTTTGGAAAACCTGAAGGGACTTGTGGATCATTTAAGAATGGCACCTGTGATGGCACAACTGCTTTGTCCATCCTTCAAAAGGAATGTGTTGGAAAAGAGTCGTGCTCAATTGATGTATCAGAAAAGAAGTTCGGTTCCACAAATTGTGGCGATATTGTGAAGAGGCTGGCCGTGGAAGCAGTTTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BetaGal_dom4_5 Gal_Lectin Glyco_hydro_35

Protein Analysis

653

Amino Acids

72.2

Weight (kDa)

5.86

Isoelectric Point (pI)

32.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	36 - 120	8.6e-36	Glycosyl hydrolases family 35
Glyco_hydro_35	PF01301	121 - 164	2.5e-13	Glycosyl hydrolases family 35
GHD	PF17834	189 - 243	1.2e-21	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	451 - 540	3.7e-25	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	578 - 653	2.8e-18	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001959)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31740 AT2G04060 AT5G20710 AT5G20710
fragaria_vesca	FvH4_1g09120 FvH4_1g09190 FvH4_1g09190 FvH4_1g26380
malus_domestica	MD02G1096400.v1.1 MD03G1276000.v1.1 MD15G1220900.v1.1 MD15G1221000.v1.1
prunus_persica	Prupe.7G194500_v2.0.a1
pyrus_communis	pycom02g07640
rosa_chinensis	RchiOBHm_Chr2g0095511 RchiOBHm_Chr2g0095591 RchiOBHm_Chr5g0030701
rosa_laevigata	RLG00000016574 RLG00000016582 RLG00000016583
rosa_multiflora	Rmu_co8462209.1_g000001 Rmu_sc0003238.1_g000025 Rmu_sc0003238.1_g000028 Rmu_sc0004719.1_g000009
rosa_roxburghii	Rroxscaffold_2G00146240
rosa_rugosa	Rorug02G0051800 Rorug02G0052600 Rorug05G0120200
rosa_samantha	Rh2AG099800 Rh2AG100400 Rh2BG101100 Rh2BG102200 Rh2CG103300 Rh2CG104600 Rh2DG100700 Rh2DG101500 Rh5AG211800 Rh5BG210700 Rh5CG234400 Rh5CG234500 Rh5DG216100
rosa_wichuraiana	Rw2G007570 Rw2G007630 Rw2G007640 Rw5G019420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1047
AasI	GACNNNNNNGTC	1 cut(s)	586
Acc16I	TGCGCA	1 cut(s)	249
AccB1I	GGYRCC	1 cut(s)	1814
AccB7I	CCANNNNNTGG	1 cut(s)	1493
AccII	CGCG	1 cut(s)	1594
AciI	CCGC	1 cut(s)	1592
AclWI	GGATC	4 cut(s)	46, 938, 1590, 1807
AcoI	YGGCCR	4 cut(s)	179, 445, 1469, 1941
AcsI	RAATTY	4 cut(s)	136, 1640, 1666, 1763
AcuI	CTGAAG	1 cut(s)	1806
AfaI	GTAC	2 cut(s)	166, 914
AfiI	CCNNNNNNNGG	5 cut(s)	467, 795, 1142, 1493, 1852
AflIII	ACRYGT	1 cut(s)	1021
AjnI	CCWGG	1 cut(s)	294
AjuI	GAANNNNNNNTTGG	2 cut(s)	1706, 1738
AleI	CACNNNNGTG	1 cut(s)	175
Alw21I	GWGCWC	2 cut(s)	1287, 1880
Alw26I	GTCTC	2 cut(s)	221, 565
AlwI	GGATC	4 cut(s)	46, 938, 1590, 1807
Ama87I	CYCGRG	1 cut(s)	809
AoxI	GGCC	5 cut(s)	179, 445, 806, 1469, 1941
ApeKI	GCWGC	5 cut(s)	60, 392, 597, 884, 1040
ApoI	RAATTY	4 cut(s)	136, 1640, 1666, 1763
ArsI	GACNNNNNNTTYG	2 cut(s)	1304, 1336
Asp700I	GAANNNNTTC	2 cut(s)	258, 789
AspA2I	CCTAGG	1 cut(s)	1384
AspLEI	GCGC	1 cut(s)	250
AspS9I	GGNCC	3 cut(s)	353, 807, 1181
AsuC2I	CCSGG	1 cut(s)	1092
AsuHPI	GGTGA	4 cut(s)	100, 511, 857, 1453
AsuNHI	GCTAGC	1 cut(s)	1769
AvaI	CYCGRG	1 cut(s)	809
AvaII	GGWCC	2 cut(s)	353, 1181
AvrII	CCTAGG	1 cut(s)	1384
BalI	TGGCCA	3 cut(s)	181, 447, 1471
BanI	GGYRCC	1 cut(s)	1814
BanII	GRGCYC	1 cut(s)	1287
BbsI	GAAGAC	4 cut(s)	229, 821, 1045, 1543
Bbv12I	GWGCWC	2 cut(s)	1287, 1880
BbvCI	CCTCAGC	1 cut(s)	771
BbvI	GCAGC	5 cut(s)	72, 404, 584, 871, 1027
BccI	CCATC	9 cut(s)	89, 110, 113, 456, 1487, 1572, 1663, 1817, 1850
BceAI	ACGGC	1 cut(s)	1928
BciT130I	CCWGG	1 cut(s)	296
BclI	TGATCA	1 cut(s)	1221
BcnI	CCSGG	1 cut(s)	1092
BcoDI	GTCTC	2 cut(s)	221, 565
BfaI	CTAG	3 cut(s)	897, 1385, 1770
BfmI	CTRYAG	2 cut(s)	58, 741
BisI	GCNGC	5 cut(s)	61, 393, 598, 885, 1041
BlnI	CCTAGG	1 cut(s)	1384
BlsI	GCNGC	5 cut(s)	62, 394, 599, 886, 1042
Bme1390I	CCNGG	2 cut(s)	296, 1092
Bme18I	GGWCC	2 cut(s)	353, 1181
BmeT110I	CYCGRG	1 cut(s)	809
BmgT120I	GGNCC	3 cut(s)	353, 807, 1181
BmiI	GGNNCC	5 cut(s)	145, 1089, 1485, 1816, 1909
BmrFI	CCNGG	2 cut(s)	296, 1092
BmsI	GCATC	4 cut(s)	208, 415, 619, 883
BmtI	GCTAGC	1 cut(s)	1773
BoxI	GACNNNNGTC	1 cut(s)	233
BpiI	GAAGAC	4 cut(s)	229, 821, 1045, 1543
BplI	GAGNNNNNCTC	2 cut(s)	1862, 1894
BpmI	CTGGAG	2 cut(s)	1191, 1470
Bpu10I	CCTNAGC	2 cut(s)	723, 771
BpuMI	CCSGG	1 cut(s)	1092
BsaJI	CCNNGG	7 cut(s)	504, 646, 1267, 1384, 1522, 1738, 1944
BsaXI	ACNNNNNCTCC	2 cut(s)	1008, 1038
Bsc4I	CCNNNNNNNGG	5 cut(s)	467, 795, 1142, 1493, 1852
Bse1I	ACTGG	6 cut(s)	257, 286, 991, 1174, 1337, 1397
Bse3DI	GCAATG	2 cut(s)	616, 1109
BseBI	CCWGG	1 cut(s)	296
BseDI	CCNNGG	7 cut(s)	504, 646, 1267, 1384, 1522, 1738, 1944
BseGI	GGATG	5 cut(s)	781, 826, 946, 1498, 1842
BseLI	CCNNNNNNNGG	5 cut(s)	467, 795, 1142, 1493, 1852
BseMI	GCAATG	2 cut(s)	616, 1109
BseMII	CTCAG	4 cut(s)	443, 762, 946, 1129
BseNI	ACTGG	6 cut(s)	257, 286, 991, 1174, 1337, 1397
BseXI	GCAGC	5 cut(s)	72, 404, 584, 871, 1027
BseYI	CCCAGC	1 cut(s)	828
Bsh1236I	CGCG	1 cut(s)	1594
BshFI	GGCC	5 cut(s)	181, 447, 808, 1471, 1943
BshNI	GGYRCC	1 cut(s)	1814
BsiHKAI	GWGCWC	2 cut(s)	1287, 1880
BsiHKCI	CYCGRG	1 cut(s)	809
BsiSI	CCGG	1 cut(s)	1092
BslFI	GGGAC	2 cut(s)	1443, 1804
BslI	CCNNNNNNNGG	5 cut(s)	467, 795, 1142, 1493, 1852
BsmAI	GTCTC	2 cut(s)	221, 565
BsmFI	GGGAC	2 cut(s)	1443, 1804
BsmI	GAATGC	1 cut(s)	250
BsnI	GGCC	5 cut(s)	181, 447, 808, 1471, 1943
BsoBI	CYCGRG	1 cut(s)	809
Bsp1286I	GDGCHC	2 cut(s)	1287, 1880
Bsp143I	GATC	5 cut(s)	51, 943, 1221, 1595, 1799
Bsp19I	CCATGG	2 cut(s)	504, 1267
BspACI	CCGC	1 cut(s)	1592
BspANI	GGCC	5 cut(s)	181, 447, 808, 1471, 1943
BspCNI	CTCAG	4 cut(s)	444, 763, 945, 1128
BspFNI	CGCG	1 cut(s)	1594
BspHI	TCATGA	1 cut(s)	487
BspLI	GGNNCC	5 cut(s)	145, 1089, 1485, 1816, 1909
BspMAI	CTGCAG	1 cut(s)	62
BspOI	GCTAGC	1 cut(s)	1773
BspPI	GGATC	4 cut(s)	46, 938, 1590, 1807
BspT107I	GGYRCC	1 cut(s)	1814
BsrDI	GCAATG	2 cut(s)	616, 1109
BsrI	ACTGG	6 cut(s)	257, 286, 991, 1174, 1337, 1397
BssECI	CCNNGG	7 cut(s)	504, 646, 1267, 1384, 1522, 1738, 1944
BssMI	GATC	5 cut(s)	51, 943, 1221, 1595, 1799
BssT1I	CCWWGG	5 cut(s)	504, 646, 1267, 1384, 1738
Bst2UI	CCWGG	1 cut(s)	296
Bst4CI	ACNGT	6 cut(s)	571, 745, 1126, 1219, 1523, 1559
Bst6I	CTCTTC	1 cut(s)	1928
BstAPI	GCANNNNNTGC	1 cut(s)	893
BstC8I	GCNNGC	3 cut(s)	1735, 1771, 1941
BstDEI	CTNAG	5 cut(s)	452, 723, 771, 932, 1115
BstDSI	CCRYGG	4 cut(s)	504, 1267, 1522, 1944
BstENI	CCTNNNNNAGG	1 cut(s)	1850
BstF5I	GGATG	5 cut(s)	781, 826, 946, 1498, 1842
BstFNI	CGCG	1 cut(s)	1594
BstHHI	GCGC	1 cut(s)	250
BstKTI	GATC	5 cut(s)	54, 946, 1224, 1598, 1802
BstMAI	GTCTC	2 cut(s)	221, 565
BstMBI	GATC	5 cut(s)	51, 943, 1221, 1595, 1799
BstMWI	GCNNNNNNNGC	5 cut(s)	606, 893, 1468, 1743, 1949
BstNI	CCWGG	1 cut(s)	296
BstNSI	RCATGY	3 cut(s)	1025, 1436, 1737
BstPAI	GACNNNNGTC	1 cut(s)	233
BstSCI	CCNGG	2 cut(s)	294, 1090
BstSFI	CTRYAG	2 cut(s)	58, 741
BstUI	CGCG	1 cut(s)	1594
BstV1I	GCAGC	5 cut(s)	72, 404, 584, 871, 1027
BstV2I	GAAGAC	4 cut(s)	229, 821, 1045, 1543
BstXI	CCANNNNNNTGG	3 cut(s)	177, 547, 836
BsuRI	GGCC	5 cut(s)	181, 447, 808, 1471, 1943
BtgI	CCRYGG	4 cut(s)	504, 1267, 1522, 1944
BtsCI	GGATG	5 cut(s)	781, 826, 946, 1498, 1842
BtsI	GCAGTG	1 cut(s)	717
BtsIMutI	CAGTG	4 cut(s)	567, 717, 750, 1123
Cac8I	GCNNGC	3 cut(s)	1735, 1771, 1941
CciI	TCATGA	1 cut(s)	487
CfoI	GCGC	1 cut(s)	250
Cfr13I	GGNCC	3 cut(s)	353, 807, 1181
CsiI	ACCWGGT	1 cut(s)	294
Csp6I	GTAC	2 cut(s)	165, 913
CspCI	CAANNNNNGTGG	2 cut(s)	556, 591
CviQI	GTAC	2 cut(s)	165, 913
DdeI	CTNAG	5 cut(s)	452, 723, 771, 932, 1115
DpnI	GATC	5 cut(s)	53, 945, 1223, 1597, 1801
DpnII	GATC	5 cut(s)	51, 943, 1221, 1595, 1799
DrdI	GACNNNNNNGTC	1 cut(s)	586
DseDI	GACNNNNNNGTC	1 cut(s)	586
EaeI	YGGCCR	4 cut(s)	179, 445, 1469, 1941
Eam1104I	CTCTTC	1 cut(s)	1928
EarI	CTCTTC	1 cut(s)	1928
Ecl136II	GAGCTC	1 cut(s)	1285
Eco130I	CCWWGG	5 cut(s)	504, 646, 1267, 1384, 1738
Eco24I	GRGCYC	1 cut(s)	1287
Eco47I	GGWCC	2 cut(s)	353, 1181
Eco53kI	GAGCTC	1 cut(s)	1285
Eco57I	CTGAAG	1 cut(s)	1806
Eco88I	CYCGRG	1 cut(s)	809
EcoICRI	GAGCTC	1 cut(s)	1285
EcoNI	CCTNNNNNAGG	1 cut(s)	1850
EcoRI	GAATTC	1 cut(s)	136
EcoRII	CCWGG	1 cut(s)	294
EcoT14I	CCWWGG	5 cut(s)	504, 646, 1267, 1384, 1738
EcoT22I	ATGCAT	1 cut(s)	1708
EcoT38I	GRGCYC	1 cut(s)	1287
ErhI	CCWWGG	5 cut(s)	504, 646, 1267, 1384, 1738
FalI	AAGNNNNNCTT	2 cut(s)	1535, 1567
FaqI	GGGAC	2 cut(s)	1443, 1804
FauNDI	CATATG	1 cut(s)	1708
FbaI	TGATCA	1 cut(s)	1221
Fnu4HI	GCNGC	5 cut(s)	61, 393, 598, 885, 1041
FokI	GGATG	5 cut(s)	788, 833, 953, 1505, 1829
FriOI	GRGCYC	1 cut(s)	1287
Fsp4HI	GCNGC	5 cut(s)	61, 393, 598, 885, 1041
FspAI	RTGCGCAY	1 cut(s)	249
FspBI	CTAG	3 cut(s)	897, 1385, 1770
FspI	TGCGCA	1 cut(s)	249
GlaI	GCGC	1 cut(s)	249
GluI	GCNGC	5 cut(s)	61, 393, 598, 885, 1041
GsaI	CCCAGC	1 cut(s)	832
GsuI	CTGGAG	2 cut(s)	1191, 1470
HaeIII	GGCC	5 cut(s)	181, 447, 808, 1471, 1943
HapII	CCGG	1 cut(s)	1092
HhaI	GCGC	1 cut(s)	250
Hin6I	GCGC	1 cut(s)	248
HinP1I	GCGC	1 cut(s)	248
HincII	GTYRAC	2 cut(s)	458, 763
HindII	GTYRAC	2 cut(s)	458, 763
HindIII	AAGCTT	1 cut(s)	1509
HinfI	GANTC	8 cut(s)	213, 316, 454, 587, 785, 1263, 1714, 1871
HpaII	CCGG	1 cut(s)	1092
HphI	GGTGA	4 cut(s)	100, 511, 857, 1453
Hpy188I	TCNGA	5 cut(s)	348, 453, 628, 1682, 1894
Hpy99I	CGWCG	2 cut(s)	267, 1406
HpyAV	CCTTC	7 cut(s)	120, 200, 320, 800, 1322, 1781, 1856
HpyCH4III	ACNGT	6 cut(s)	571, 745, 1126, 1219, 1523, 1559
HpyCH4IV	ACGT	3 cut(s)	84, 1050, 1062
HpyF10VI	GCNNNNNNNGC	5 cut(s)	606, 893, 1468, 1743, 1949
HpyF3I	CTNAG	5 cut(s)	452, 723, 771, 932, 1115
HpySE526I	ACGT	3 cut(s)	84, 1050, 1062
HspAI	GCGC	1 cut(s)	248
Ksp22I	TGATCA	1 cut(s)	1221
Kzo9I	GATC	5 cut(s)	51, 943, 1221, 1595, 1799
LmnI	GCTCC	2 cut(s)	1384, 1489
Lsp1109I	GCAGC	5 cut(s)	72, 404, 584, 871, 1027
LweI	GCATC	4 cut(s)	208, 415, 619, 883
MabI	ACCWGGT	1 cut(s)	294
MaeI	CTAG	3 cut(s)	897, 1385, 1770
MaeII	ACGT	3 cut(s)	84, 1050, 1062
MaeIII	GTNAC	4 cut(s)	551, 989, 1233, 1514
MalI	GATC	5 cut(s)	53, 945, 1223, 1597, 1801
MboI	GATC	5 cut(s)	51, 943, 1221, 1595, 1799
MboII	GAAGA	8 cut(s)	229, 335, 680, 826, 989, 1045, 1543, 1945
MfeI	CAATTG	3 cut(s)	222, 1190, 1881
MhlI	GDGCHC	2 cut(s)	1287, 1880
MlsI	TGGCCA	3 cut(s)	181, 447, 1471
MluNI	TGGCCA	3 cut(s)	181, 447, 1471
MlyI	GAGTC	5 cut(s)	207, 463, 596, 1257, 1880
MmeI	TCCRAC	5 cut(s)	1108, 1177, 1240, 1666, 1843
Mox20I	TGGCCA	3 cut(s)	181, 447, 1471
Mph1103I	ATGCAT	1 cut(s)	1708
MroXI	GAANNNNTTC	2 cut(s)	258, 789
MscI	TGGCCA	3 cut(s)	181, 447, 1471
MseI	TTAA	2 cut(s)	1011, 1806
MslI	CAYNNNNRTG	2 cut(s)	175, 1005
Msp20I	TGGCCA	3 cut(s)	181, 447, 1471
MspI	CCGG	1 cut(s)	1092
MspR9I	CCNGG	2 cut(s)	296, 1092
MunI	CAATTG	3 cut(s)	222, 1190, 1881
Mva1269I	GAATGC	1 cut(s)	250
MvaI	CCWGG	1 cut(s)	296
MvnI	CGCG	1 cut(s)	1594
MwoI	GCNNNNNNNGC	5 cut(s)	606, 893, 1468, 1743, 1949
NciI	CCSGG	1 cut(s)	1092
NcoI	CCATGG	2 cut(s)	504, 1267
NdeI	CATATG	1 cut(s)	1708
NdeII	GATC	5 cut(s)	51, 943, 1221, 1595, 1799
NheI	GCTAGC	1 cut(s)	1769
NlaIV	GGNNCC	5 cut(s)	145, 1089, 1485, 1816, 1909
NmuCI	GTSAC	2 cut(s)	1233, 1514
NsbI	TGCGCA	1 cut(s)	249
NsiI	ATGCAT	1 cut(s)	1708
NspI	RCATGY	3 cut(s)	1025, 1436, 1737
OliI	CACNNNNGTG	1 cut(s)	175
PaeI	GCATGC	1 cut(s)	1737
PagI	TCATGA	1 cut(s)	487
PciI	ACATGT	1 cut(s)	1021
PctI	GAATGC	1 cut(s)	250
PdmI	GAANNNNTTC	2 cut(s)	258, 789
PfeI	GAWTC	3 cut(s)	316, 785, 1714
PflMI	CCANNNNNTGG	1 cut(s)	1493
PfoI	TCCNGGA	1 cut(s)	1090
PkrI	GCNGC	5 cut(s)	62, 394, 599, 886, 1042
PleI	GAGTC	5 cut(s)	207, 462, 595, 1257, 1879
PpsI	GAGTC	5 cut(s)	207, 462, 595, 1257, 1879
PscI	ACATGT	1 cut(s)	1021
PshAI	GACNNNNGTC	1 cut(s)	233
PsiI	TTATAA	1 cut(s)	1047
Psp124BI	GAGCTC	1 cut(s)	1287
Psp6I	CCWGG	1 cut(s)	294
PspFI	CCCAGC	1 cut(s)	828
PspGI	CCWGG	1 cut(s)	294
PspN4I	GGNNCC	5 cut(s)	145, 1089, 1485, 1816, 1909
PspPI	GGNCC	3 cut(s)	353, 807, 1181
PstI	CTGCAG	1 cut(s)	62
RsaI	GTAC	2 cut(s)	166, 914
RsaNI	GTAC	2 cut(s)	165, 913
RseI	CAYNNNNRTG	2 cut(s)	175, 1005
SacI	GAGCTC	1 cut(s)	1287
SaqAI	TTAA	2 cut(s)	1011, 1806
SatI	GCNGC	5 cut(s)	61, 393, 598, 885, 1041
Sau3AI	GATC	5 cut(s)	51, 943, 1221, 1595, 1799
Sau96I	GGNCC	3 cut(s)	353, 807, 1181
SchI	GAGTC	5 cut(s)	207, 463, 596, 1257, 1880
ScrFI	CCNGG	2 cut(s)	296, 1092
SduI	GDGCHC	2 cut(s)	1287, 1880
SexAI	ACCWGGT	1 cut(s)	294
SfaNI	GCATC	4 cut(s)	208, 415, 619, 883
SfcI	CTRYAG	2 cut(s)	58, 741
SinI	GGWCC	2 cut(s)	353, 1181
SmiMI	CAYNNNNRTG	2 cut(s)	175, 1005
SphI	GCATGC	1 cut(s)	1737
SsiI	CCGC	1 cut(s)	1592
SspI	AATATT	1 cut(s)	532
SspMI	CTAG	3 cut(s)	897, 1385, 1770
SstI	GAGCTC	1 cut(s)	1287
StyD4I	CCNGG	2 cut(s)	294, 1090
StyI	CCWWGG	5 cut(s)	504, 646, 1267, 1384, 1738
TaaI	ACNGT	6 cut(s)	571, 745, 1126, 1219, 1523, 1559
TaiI	ACGT	3 cut(s)	87, 1053, 1065
TaqI	TCGA	3 cut(s)	160, 216, 1068
TatI	WGTACW	1 cut(s)	164
TfiI	GAWTC	3 cut(s)	316, 785, 1714
Tru1I	TTAA	2 cut(s)	1011, 1806
Tru9I	TTAA	2 cut(s)	1011, 1806
TscAI	CASTG	4 cut(s)	574, 724, 750, 1123
TseFI	GTSAC	2 cut(s)	1233, 1514
TseI	GCWGC	5 cut(s)	60, 392, 597, 884, 1040
Tsp45I	GTSAC	2 cut(s)	1233, 1514
TspGWI	ACGGA	1 cut(s)	833
TspRI	CASTG	4 cut(s)	574, 724, 750, 1123
Van91I	CCANNNNNTGG	1 cut(s)	1493
VpaK11BI	GGWCC	2 cut(s)	353, 1181
XagI	CCTNNNNNAGG	1 cut(s)	1850
XapI	RAATTY	4 cut(s)	136, 1640, 1666, 1763
XceI	RCATGY	3 cut(s)	1025, 1436, 1737
XcmI	CCANNNNNNNNNTGG	1 cut(s)	544
XmaJI	CCTAGG	1 cut(s)	1384
XmnI	GAANNNNTTC	2 cut(s)	258, 789
XspI	CTAG	3 cut(s)	897, 1385, 1770
Zsp2I	ATGCAT	1 cut(s)	1708

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.