Rh5CG234500

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001959
Physical Location & Seq
Forward (+)
24395126 .. 24399702
4577 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG234500.1

Sequence Viewer

Length: 2499 bp
ATGCCTTCAAATGGGTTTTTCTTTTTTGTTTCTGCTCTCTTACTGTGTCAACTGTGTTCCGCCCTGCCCGTGGAGGTCGATTACAATGGAAGAGCCATCACAATTGATGGTGATAGAAGAATCATAATCTCTGGCTCTATTCACTACCCAAGGAGTACTCCTGAAATGTGGCCTGACTTGATCCAAAAAGCAAAAGATGGAGGCCTTAATGCTATTGATACTTACGTCTTTTGGAACGCACATGAATCTATGCGTCGGCAGTACGATTTCTCAGGAAACTTGGACTTGGTGAGATTCATTCAGACCATCCAAAAGGCAGGGCTCTATGCAATTCTTCGGATTGGACCATATGTTTGTGCAGAATGGAATTATGGTGGTTTTCCTGTGTGGTTGCATAACATTCCAGGCATCAAACTGAGGACAAACAATGATATCTATAAGAATGAGATGCAAACCTTTACAACTTTGATAGTGGACATGATGAAGCATGAGAATCTTTTTGCTTCACAAGGCGGACCTGTTATAATTTCTCAGATTGAAAATGAGTATGGGAATGTTATTGGCCCGTACGGCAACCAAGGAAAGCAATATCTACAATGGTGTGCTGATTTAGCTGAATCTTACCAGATTGGTGTACCATGGATTATGTGTCAACAGAAAGATGCTCCACAACCAATTATTAATACCTGTAATGGCTGGTATTGTGATCAATTTCAACCTTCCTACAATACTAGTCCGAAGATGTGGACAGAAAATTGGACTGGATGGTTCAAGAATTGGGGCATGCAAGATCCACATAGAACTGCAGAGGATCTTGCCTTTTCCGTTGCACGATTCTTTCAATTACAAGGCACCTTCCAAAACTATTATATGTACCATGGAGGAACAAATTTTGGTCGGTCAGCAGGTGGTCCTTACATCACCACATCATACGATTATGATGCACCATTGGATGAATATGGAAATTTGAATCAACCCAAGTGGGGGCATCTTAAAGAGCTTCATAACCTCATTAGATCAATGGAGAAGGTTCTGACTTATGGTTTCGTAAGAGAGGTTGAATACGGAGAGAGTTCGCATGTAACAGTTTACAGTTATGGGGGAAATCGGTCCTGTTTCATTAGTAACGCAAACAGAACCACTGATATAACAATTAACTTTGAGAACTTTATGTATTCTGTTCCTGCTTGGTCTGTGACTATACTTCCTGATTGCCATACCGATGTGTACAACACTGCTCAGGTTAATGTGCAAACATCAATAATGGAGAAGGTGCTAAACCAAGCCGATACCAGGGTAGGGGAACCCTATGAGTTGATTTGGGGGTGGAGGCCTGAGAACCTCAACGAAAATGGCTTAGTTCTGCATAGCAATCTCACTACTAATCAGCTGCTGGATCAAAAAGTAGTCACGAATGATACCAGTGATTACTTATGGTACATCACCAGTCTGGATCACAACGCTATTGATCCCAATTGGACCAGCAAAGAACTTACGCTAAGAGTGAATAGCAGAGGACATATCCTCCATGCTTTTATTAATGGCGAACACATAGGGACTGAAGTTTCTGGACAAGAGTTCAATCCTTTTACGTTAGAGAGAAGAATTGAGCTACAGCATGACAAGAATGATTTATCTCTTCTCAGTGTCACTGTTGGCTTGAAACATTACAATCCATTTTTTGACGAAACAGATGTTGGGATTCATGGTCCTGTTCAACTGATTGGCAAGTACAAGAATGGTACTGAGGTGATAAAGGACTTGAGCAAAAATGAGTGGACATATAAGGTTGGACTTGCTGGTGAACAAAAGGGACTTTATCAAATTACTGGTCATGCTGCTAGTTTTCGCTGGACTACAAATAATCTTCCAACAGATAGGATGTTTGTTTGGTACAAGACTATCTTCAAAGCTCCTCTTGGAACCGATCCGGTTGTGGTCGACTTAAGGGGTCTAGGAAAGGGTCATGCTTGGGTGAATGGCCGAAGCATTGGCAGGTATTGGACAAGTTACACTGCCGATGAAAATGGTTGTACAGAAAAATGCGACTATCGTGGAACTTACAGCAATAGGAAGTGCTTGACTAATTGTGGGAAATCTTCCCAGAGATGGTACCATATTCCGCGATCTTTCCTCCAAGACGATAACAATGCACTAATTCTGTTCGAAGAGTTTGGAGGCAATCCATTTAATGTTAAGTTCCAGACTGTAACTGTTGCTAAAGCTTGCGCAAATGTTTACGAAGGTAAGGTACTGCAGTTGTCATGCCAAGGAGGAAGAGTTCTATCAAATGTTAGGGTTGCCATCTTCGGTGATGCACAAGGCGCTTGCAGTAGATCCTTCAGGAAAGGCCAGTGTGAATCTCCGACAGCTTTATCATACATCCAAAAGGCATGCATTGGCAAAGTGGAATGTTATATCGATGTTTCTGAGACGACTCTTGGACCAACGGGCTGCAAACACATAAATCGCCTTGCTGTTGAAGTCGATTGTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gal_Lectin Glyco_hydro_35

Protein Analysis

832

Amino Acids

94.53

Weight (kDa)

6.08

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 33 - 336 2.8e-120 Glycosyl hydrolases family 35
GHD PF17834 361 - 415 1.4e-19 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 630 - 717 6.6e-22 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 754 - 831 1.4e-12 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 524
AarI CACCTGC 1 cut(s) 896
Acc16I TGCGCA 1 cut(s) 2230
Acc36I ACCTGC 2 cut(s) 896, 1986
Acc65I GGTACC 1 cut(s) 2112
AccB1I GGYRCC 2 cut(s) 851, 2112
AccI GTMKAC 1 cut(s) 1941
AccII CGCG 1 cut(s) 2125
AciI CCGC 3 cut(s) 60, 513, 2123
AclWI GGATC 8 cut(s) 175, 785, 819, 1404, 1461, 1463, 1922, 2331
AcoI YGGCCR 1 cut(s) 1981
AcsI RAATTY 2 cut(s) 889, 964
AcuI CTGAAG 2 cut(s) 1581, 2326
AfiI CCNNNNNNNGG 7 cut(s) 11, 70, 983, 984, 1293, 1299, 2109
AflII CTTAAG 1 cut(s) 1945
AhlI ACTAGT 1 cut(s) 731
AjnI CCWGG 2 cut(s) 403, 1292
AjuI GAANNNNNNNTTGG 2 cut(s) 1680, 1712
AloI GAACNNNNNNTCC 2 cut(s) 2265, 2297
AluBI AGCT 7 cut(s) 614, 1000, 1390, 1612, 1913, 2225, 2372
AluI AGCT 7 cut(s) 614, 1000, 1390, 1612, 1913, 2225, 2372
Alw26I GTCTC 1 cut(s) 2426
AlwI GGATC 8 cut(s) 175, 785, 819, 1404, 1461, 1463, 1922, 2331
AlwNI CAGNNNCTG 1 cut(s) 1393
AoxI GGCC 6 cut(s) 170, 202, 562, 1331, 1981, 2350
ApeKI GCWGC 3 cut(s) 1390, 1838, 2454
ApoI RAATTY 2 cut(s) 889, 964
ArsI GACNNNNNNTTYG 4 cut(s) 336, 368, 1816, 1848
AseI ATTAAT 2 cut(s) 681, 1539
Asp718I GGTACC 1 cut(s) 2112
AspLEI GCGC 2 cut(s) 2231, 2327
AspS9I GGNCC 8 cut(s) 344, 515, 563, 911, 1110, 1479, 1709, 2444
AsuHPI GGTGA 8 cut(s) 122, 301, 913, 1435, 1762, 1814, 1987, 2324
AsuII TTCGAA 1 cut(s) 2166
AvaII GGWCC 7 cut(s) 344, 515, 911, 1110, 1479, 1709, 2444
BaeI ACNNNNGTAYC 8 cut(s) 210, 243, 1281, 1314, 1734, 1767, 2243, 2276
BanI GGYRCC 2 cut(s) 851, 2112
BanII GRGCYC 1 cut(s) 324
BarI GAAGNNNNNNTAC 2 cut(s) 2235, 2267
BbvI GCAGC 3 cut(s) 1377, 1825, 2441
BccI CCATC 7 cut(s) 101, 104, 191, 314, 759, 2103, 2312
BceAI ACGGC 1 cut(s) 586
BcgI CGANNNNNNTGC 4 cut(s) 923, 957, 2132, 2166
BciT130I CCWGG 2 cut(s) 405, 1294
BclI TGATCA 1 cut(s) 706
BcoDI GTCTC 1 cut(s) 2426
BcuI ACTAGT 1 cut(s) 731
BfaI CTAG 4 cut(s) 732, 1842, 1955, 2497
BfmI CTRYAG 3 cut(s) 804, 1613, 2255
BfoI RGCGCY 1 cut(s) 2328
BfrI CTTAAG 1 cut(s) 1945
BfuAI ACCTGC 2 cut(s) 896, 1986
BglI GCCNNNNNGGC 1 cut(s) 570
BisI GCNGC 3 cut(s) 1391, 1839, 2455
BlsI GCNGC 3 cut(s) 1392, 1840, 2456
BmcAI AGTACT 1 cut(s) 157
Bme1390I CCNGG 2 cut(s) 405, 1294
Bme18I GGWCC 7 cut(s) 344, 515, 911, 1110, 1479, 1709, 2444
BmgT120I GGNCC 8 cut(s) 344, 515, 563, 911, 1110, 1479, 1709, 2444
BmiI GGNNCC 4 cut(s) 853, 1305, 1924, 2114
BmrFI CCNGG 2 cut(s) 405, 1294
BmsI GCATC 6 cut(s) 417, 438, 652, 931, 997, 2305
Bpu10I CCTNAGC 1 cut(s) 1239
Bpu14I TTCGAA 1 cut(s) 2166
BpuEI CTTGAG 1 cut(s) 1783
Bsa29I ATCGAT 1 cut(s) 2421
BsaJI CCNNGG 7 cut(s) 69, 149, 577, 638, 877, 1293, 2269
BsaWI WCCGGW 1 cut(s) 1930
BsaXI ACNNNNNCTCC 4 cut(s) 1509, 1539, 2265, 2295
Bsc4I CCNNNNNNNGG 7 cut(s) 11, 70, 983, 984, 1293, 1299, 2109
Bse1I ACTGG 5 cut(s) 766, 1422, 1446, 1834, 2353
BseBI CCWGG 2 cut(s) 405, 1294
BseCI ATCGAT 1 cut(s) 2421
BseDI CCNNGG 7 cut(s) 69, 149, 577, 638, 877, 1293, 2269
BseGI GGATG 5 cut(s) 306, 770, 958, 1887, 2382
BseLI CCNNNNNNNGG 7 cut(s) 11, 70, 983, 984, 1293, 1299, 2109
BseMII CTCAG 8 cut(s) 285, 407, 545, 1253, 1326, 1657, 1737, 2421
BseNI ACTGG 5 cut(s) 766, 1422, 1446, 1834, 2353
BseRI GAGGAG 1 cut(s) 1905
BseXI GCAGC 3 cut(s) 1377, 1825, 2441
BsgI GTGCAG 1 cut(s) 378
Bsh1236I CGCG 1 cut(s) 2125
BshFI GGCC 6 cut(s) 172, 204, 564, 1333, 1983, 2352
BshNI GGYRCC 2 cut(s) 851, 2112
BshVI ATCGAT 1 cut(s) 2421
BsiSI CCGG 1 cut(s) 1931
BsiWI CGTACG 1 cut(s) 567
BslFI GGGAC 2 cut(s) 1570, 1827
BslI CCNNNNNNNGG 7 cut(s) 11, 70, 983, 984, 1293, 1299, 2109
BsmAI GTCTC 1 cut(s) 2426
BsmBI CGTCTC 1 cut(s) 2426
BsmFI GGGAC 2 cut(s) 1570, 1827
BsnI GGCC 6 cut(s) 172, 204, 564, 1333, 1983, 2352
Bsp119I TTCGAA 1 cut(s) 2166
Bsp1286I GDGCHC 1 cut(s) 324
Bsp1407I TGTACA 2 cut(s) 1227, 2033
Bsp19I CCATGG 2 cut(s) 638, 877
BspACI CCGC 3 cut(s) 60, 513, 2123
BspANI GGCC 6 cut(s) 172, 204, 564, 1333, 1983, 2352
BspCNI CTCAG 8 cut(s) 284, 408, 544, 1252, 1327, 1656, 1738, 2422
BspDI ATCGAT 1 cut(s) 2421
BspFNI CGCG 1 cut(s) 2125
BspLI GGNNCC 4 cut(s) 853, 1305, 1924, 2114
BspMAI CTGCAG 2 cut(s) 808, 2259
BspMI ACCTGC 2 cut(s) 896, 1986
BspPI GGATC 8 cut(s) 175, 785, 819, 1404, 1461, 1463, 1922, 2331
BspQI GCTCTTC 1 cut(s) 85
BspT104I TTCGAA 1 cut(s) 2166
BspT107I GGYRCC 2 cut(s) 851, 2112
BspTI CTTAAG 1 cut(s) 1945
BsrGI TGTACA 2 cut(s) 1227, 2033
BsrI ACTGG 5 cut(s) 766, 1422, 1446, 1834, 2353
BssECI CCNNGG 7 cut(s) 69, 149, 577, 638, 877, 1293, 2269
BssT1I CCWWGG 5 cut(s) 149, 577, 638, 877, 2269
Bst2UI CCWGG 2 cut(s) 405, 1294
Bst4CI ACNGT 7 cut(s) 45, 54, 1087, 1094, 1654, 2209, 2215
Bst6I CTCTTC 4 cut(s) 85, 1644, 2163, 2272
BstAFI CTTAAG 1 cut(s) 1945
BstAUI TGTACA 2 cut(s) 1227, 2033
BstBI TTCGAA 1 cut(s) 2166
BstC8I GCNNGC 4 cut(s) 785, 2227, 2329, 2395
BstDSI CCRYGG 3 cut(s) 69, 638, 877
BstF5I GGATG 5 cut(s) 306, 770, 958, 1887, 2382
BstFNI CGCG 1 cut(s) 2125
BstH2I RGCGCY 1 cut(s) 2328
BstHHI GCGC 2 cut(s) 2231, 2327
BstMAI GTCTC 1 cut(s) 2426
BstMWI GCNNNNNNNGC 3 cut(s) 570, 611, 2324
BstNI CCWGG 2 cut(s) 405, 1294
BstNSI RCATGY 3 cut(s) 787, 1082, 2397
BstSCI CCNGG 2 cut(s) 403, 1292
BstSFI CTRYAG 3 cut(s) 804, 1613, 2255
BstUI CGCG 1 cut(s) 2125
BstV1I GCAGC 3 cut(s) 1377, 1825, 2441
BstX2I RGATCY 3 cut(s) 790, 811, 2336
BstYI RGATCY 3 cut(s) 790, 811, 2336
Bsu15I ATCGAT 1 cut(s) 2421
BsuRI GGCC 6 cut(s) 172, 204, 564, 1333, 1983, 2352
BsuTUI ATCGAT 1 cut(s) 2421
BtgI CCRYGG 3 cut(s) 69, 638, 877
BtsCI GGATG 5 cut(s) 306, 770, 958, 1887, 2382
BtsI GCAGTG 2 cut(s) 1233, 2013
BtsIMutI CAGTG 7 cut(s) 1140, 1233, 1429, 1650, 1651, 2013, 2360
BveI ACCTGC 2 cut(s) 896, 1986
Cac8I GCNNGC 4 cut(s) 785, 2227, 2329, 2395
CaiI CAGNNNCTG 1 cut(s) 1393
CfoI GCGC 2 cut(s) 2231, 2327
Cfr13I GGNCC 8 cut(s) 344, 515, 563, 911, 1110, 1479, 1709, 2444
ClaI ATCGAT 1 cut(s) 2421
CseI GACGC 1 cut(s) 242
CspCI CAANNNNNGTGG 2 cut(s) 962, 997
EaeI YGGCCR 1 cut(s) 1981
Eam1104I CTCTTC 4 cut(s) 85, 1644, 2163, 2272
EarI CTCTTC 4 cut(s) 85, 1644, 2163, 2272
EciI GGCGGA 2 cut(s) 49, 528
Eco130I CCWWGG 5 cut(s) 149, 577, 638, 877, 2269
Eco147I AGGCCT 2 cut(s) 204, 1333
Eco24I GRGCYC 1 cut(s) 324
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 7 cut(s) 344, 515, 911, 1110, 1479, 1709, 2444
Eco57I CTGAAG 2 cut(s) 1581, 2326
EcoRII CCWGG 2 cut(s) 403, 1292
EcoRV GATATC 1 cut(s) 433
EcoT14I CCWWGG 5 cut(s) 149, 577, 638, 877, 2269
EcoT22I ATGCAT 1 cut(s) 2399
EcoT38I GRGCYC 1 cut(s) 324
ErhI CCWWGG 5 cut(s) 149, 577, 638, 877, 2269
Esp3I CGTCTC 1 cut(s) 2426
FalI AAGNNNNNCTT 4 cut(s) 1889, 1921, 1902, 1934
FaqI GGGAC 2 cut(s) 1570, 1827
FauNDI CATATG 1 cut(s) 349
FbaI TGATCA 1 cut(s) 706
FblI GTMKAC 1 cut(s) 1941
Fnu4HI GCNGC 3 cut(s) 1391, 1839, 2455
FokI GGATG 5 cut(s) 293, 777, 965, 1894, 2369
FriOI GRGCYC 1 cut(s) 324
Fsp4HI GCNGC 3 cut(s) 1391, 1839, 2455
FspBI CTAG 4 cut(s) 732, 1842, 1955, 2497
FspI TGCGCA 1 cut(s) 2230
GlaI GCGC 2 cut(s) 2230, 2326
GluI GCNGC 3 cut(s) 1391, 1839, 2455
HaeII RGCGCY 1 cut(s) 2328
HaeIII GGCC 6 cut(s) 172, 204, 564, 1333, 1983, 2352
HapII CCGG 1 cut(s) 1931
HgaI GACGC 1 cut(s) 242
HhaI GCGC 2 cut(s) 2231, 2327
Hin6I GCGC 2 cut(s) 2229, 2325
HinP1I GCGC 2 cut(s) 2229, 2325
HincII GTYRAC 3 cut(s) 50, 653, 1942
HindII GTYRAC 3 cut(s) 50, 653, 1942
HindIII AAGCTT 1 cut(s) 2223
HpaII CCGG 1 cut(s) 1931
HphI GGTGA 8 cut(s) 122, 301, 913, 1435, 1762, 1814, 1987, 2324
Hpy188I TCNGA 7 cut(s) 303, 339, 534, 738, 1035, 2367, 2431
Hpy188III TCNNGA 9 cut(s) 161, 273, 772, 1208, 1411, 1451, 1569, 2203, 2344
Hpy99I CGWCG 1 cut(s) 258
HpyAV CCTTC 7 cut(s) 15, 729, 865, 1021, 1264, 2237, 2350
HpyCH4III ACNGT 7 cut(s) 45, 54, 1087, 1094, 1654, 2209, 2215
HpyCH4IV ACGT 2 cut(s) 225, 1592
HpyF10VI GCNNNNNNNGC 3 cut(s) 570, 611, 2324
HpySE526I ACGT 2 cut(s) 225, 1592
HspAI GCGC 2 cut(s) 2229, 2325
KpnI GGTACC 1 cut(s) 2116
Ksp22I TGATCA 1 cut(s) 706
LguI GCTCTTC 1 cut(s) 85
LmnI GCTCC 2 cut(s) 670, 1918
Lsp1109I GCAGC 3 cut(s) 1377, 1825, 2441
LweI GCATC 6 cut(s) 417, 438, 652, 931, 997, 2305
MaeI CTAG 4 cut(s) 732, 1842, 1955, 2497
MaeII ACGT 2 cut(s) 225, 1592
MaeIII GTNAC 8 cut(s) 1081, 1124, 1195, 1408, 1648, 2009, 2209, 2492
MfeI CAATTG 2 cut(s) 102, 1474
MflI RGATCY 3 cut(s) 790, 811, 2336
MhlI GDGCHC 1 cut(s) 324
MlyI GAGTC 1 cut(s) 2431
MmeI TCCRAC 3 cut(s) 1771, 1895, 2390
Mph1103I ATGCAT 1 cut(s) 2399
MseI TTAA 9 cut(s) 207, 681, 993, 1155, 1245, 1539, 1946, 2190, 2196
MslI CAYNNNNRTG 1 cut(s) 1221
MspA1I CMGCKG 1 cut(s) 1390
MspCI CTTAAG 1 cut(s) 1945
MspI CCGG 1 cut(s) 1931
MspR9I CCNGG 2 cut(s) 405, 1294
MunI CAATTG 2 cut(s) 102, 1474
MvaI CCWGG 2 cut(s) 405, 1294
MvnI CGCG 1 cut(s) 2125
MwoI GCNNNNNNNGC 3 cut(s) 570, 611, 2324
NcoI CCATGG 2 cut(s) 638, 877
NdeI CATATG 1 cut(s) 349
NlaIV GGNNCC 4 cut(s) 853, 1305, 1924, 2114
NmuCI GTSAC 3 cut(s) 1195, 1408, 1648
NsbI TGCGCA 1 cut(s) 2230
NsiI ATGCAT 1 cut(s) 2399
NspI RCATGY 3 cut(s) 787, 1082, 2397
NspV TTCGAA 1 cut(s) 2166
PaeI GCATGC 2 cut(s) 787, 2397
PaqCI CACCTGC 1 cut(s) 896
PceI AGGCCT 2 cut(s) 204, 1333
PciSI GCTCTTC 1 cut(s) 85
PfeI GAWTC 9 cut(s) 120, 245, 294, 493, 617, 834, 970, 1702, 2360
Pfl23II CGTACG 1 cut(s) 567
PkrI GCNGC 3 cut(s) 1392, 1840, 2456
PleI GAGTC 1 cut(s) 2431
PpsI GAGTC 1 cut(s) 2431
PshBI ATTAAT 2 cut(s) 681, 1539
PsiI TTATAA 1 cut(s) 524
Psp6I CCWGG 2 cut(s) 403, 1292
PspGI CCWGG 2 cut(s) 403, 1292
PspLI CGTACG 1 cut(s) 567
PspN4I GGNNCC 4 cut(s) 853, 1305, 1924, 2114
PspPI GGNCC 8 cut(s) 344, 515, 563, 911, 1110, 1479, 1709, 2444
PstI CTGCAG 2 cut(s) 808, 2259
PstNI CAGNNNCTG 1 cut(s) 1393
PsuI RGATCY 3 cut(s) 790, 811, 2336
PvuII CAGCTG 1 cut(s) 1390
RseI CAYNNNNRTG 1 cut(s) 1221
SalI GTCGAC 1 cut(s) 1940
SapI GCTCTTC 1 cut(s) 85
SaqAI TTAA 9 cut(s) 207, 681, 993, 1155, 1245, 1539, 1946, 2190, 2196
SatI GCNGC 3 cut(s) 1391, 1839, 2455
Sau96I GGNCC 8 cut(s) 344, 515, 563, 911, 1110, 1479, 1709, 2444
ScaI AGTACT 1 cut(s) 157
SchI GAGTC 1 cut(s) 2431
ScrFI CCNGG 2 cut(s) 405, 1294
SduI GDGCHC 1 cut(s) 324
SfaNI GCATC 6 cut(s) 417, 438, 652, 931, 997, 2305
SfcI CTRYAG 3 cut(s) 804, 1613, 2255
SfuI TTCGAA 1 cut(s) 2166
SinI GGWCC 7 cut(s) 344, 515, 911, 1110, 1479, 1709, 2444
SmiMI CAYNNNNRTG 1 cut(s) 1221
SmlI CTYRAG 2 cut(s) 1762, 1945
SmoI CTYRAG 2 cut(s) 1762, 1945
SpeI ACTAGT 1 cut(s) 731
SphI GCATGC 2 cut(s) 787, 2397
SseBI AGGCCT 2 cut(s) 204, 1333
SsiI CCGC 3 cut(s) 60, 513, 2123
SspMI CTAG 4 cut(s) 732, 1842, 1955, 2497
StuI AGGCCT 2 cut(s) 204, 1333
StyD4I CCNGG 2 cut(s) 403, 1292
StyI CCWWGG 5 cut(s) 149, 577, 638, 877, 2269
TaaI ACNGT 7 cut(s) 45, 54, 1087, 1094, 1654, 2209, 2215
TaiI ACGT 2 cut(s) 228, 1595
TaqI TCGA 5 cut(s) 78, 1941, 2166, 2421, 2487
TaqII GACCGA 2 cut(s) 888, 1098
TatI WGTACW 4 cut(s) 155, 1227, 1731, 2033
TfiI GAWTC 9 cut(s) 120, 245, 294, 493, 617, 834, 970, 1702, 2360
Tru1I TTAA 9 cut(s) 207, 681, 993, 1155, 1245, 1539, 1946, 2190, 2196
Tru9I TTAA 9 cut(s) 207, 681, 993, 1155, 1245, 1539, 1946, 2190, 2196
TscAI CASTG 7 cut(s) 1147, 1240, 1429, 1651, 1657, 2020, 2360
TseFI GTSAC 3 cut(s) 1195, 1408, 1648
TseI GCWGC 3 cut(s) 1390, 1838, 2454
Tsp45I GTSAC 3 cut(s) 1195, 1408, 1648
TspDTI ATGAA 8 cut(s) 258, 286, 497, 969, 992, 1108, 1694, 2037
TspGWI ACGGA 2 cut(s) 814, 1080
TspRI CASTG 7 cut(s) 1147, 1240, 1429, 1651, 1657, 2020, 2360
Vha464I CTTAAG 1 cut(s) 1945
VpaK11BI GGWCC 7 cut(s) 344, 515, 911, 1110, 1479, 1709, 2444
VspI ATTAAT 2 cut(s) 681, 1539
XapI RAATTY 2 cut(s) 889, 964
XceI RCATGY 3 cut(s) 787, 1082, 2397
XmiI GTMKAC 1 cut(s) 1941
XspI CTAG 4 cut(s) 732, 1842, 1955, 2497
ZrmI AGTACT 1 cut(s) 157
Zsp2I ATGCAT 1 cut(s) 2399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.