MD02G1096400.v1.1

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001959
Physical Location & Seq
Forward (+)
7620293 .. 7622687
2395 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1096400.v1.1.491

Sequence Viewer

Length: 927 bp
ATGCTCAAACGAACAATAGGACTTTCGTTTAGTAGAGAGCGTGTAATTTGTGAGAGCAGTTCGAGGAAATACGTACACCATTTCCTGCCTGGTCTGTTGGTCAACACACAAACAACAGTTAAGGTGAAGGTAGTGAACAAAGCTGAAGATGAACCCGAATCATTTGCTTGGTCGTGGAGGCCTGAGACCATTGATGACACCAGACTCCTTGCAATGGGAGAAGGTACTGCAAATCAACTTATGGATCAAAAAGAAGCTGGAAACGATTCTGGTGATTCTCTTTGGTACATGACAAGGTCGTCGTACCCAGTGCACAAGGCTCCCGCTTTACGCAGGGTCTGGGAGAGTGTTCATCTCAAGAAATATGAACCAATCTGGAGCAAGAATATGGCTTTAAGGGTCAATGACGCTGGTTTTATTCTTCTTGCATATGTCAAAGGGGAATATGTTGGTTCTCAATGGGCTCAATACTACGTCTTCAACTATGTTTTCGAGAAAAATGTCAAGTTACATCCTGGAAAGAACATTATTTCATTGTGGAGTGCTACTGTTGGATTTCCGGCAACCTTCAAAGCTCCTCTTGGAACTGATCCAATTGCCGTGGACTTACAAGGTAAAGGTCACGCTTGGATCAGTGGTCATAGTATCGGGCGATATTGGCCGAGTTATTTGTCATACGACACTGGTTGTAGTCTCGATGCTTGTGCTTACTGTGGTTCATATGATAACAAGAAGTATTCCTTCAACTGTGGCAATCCTACCCAAAGATGGTACCATGTTCCTCGATCTTTTATGCAAGACGGGGAAAATACAATTGTTTTGTTTGAGGAGTTTGGTGGCAATCCATCTCTTGTGAATTTCCTAACTATCCAAGTTGGAGCTGTCTGTGCCCATGCATATGAGAATCACACAGTGGAATTGGCATGA

Protein Analysis

309

Amino Acids

34.84

Weight (kDa)

7.65

Isoelectric Point (pI)

36.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BetaGal_gal-bd PF21467 188 - 270 4.3e-16 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 298
Acc65I GGTACC 1 cut(s) 771
AccB1I GGYRCC 1 cut(s) 771
AciI CCGC 1 cut(s) 324
AclWI GGATC 3 cut(s) 252, 584, 638
AcoI YGGCCR 1 cut(s) 659
AcsI RAATTY 1 cut(s) 856
AcuI CTGAAG 1 cut(s) 165
AdeI CACNNNGTG 1 cut(s) 913
AfaI GTAC 5 cut(s) 75, 226, 287, 305, 773
AfiI CCNNNNNNNGG 2 cut(s) 214, 768
AgsI TTSAA 3 cut(s) 481, 571, 745
AjnI CCWGG 2 cut(s) 88, 514
AluBI AGCT 4 cut(s) 143, 257, 575, 881
AluI AGCT 4 cut(s) 143, 257, 575, 881
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 179, 698
Alw44I GTGCAC 1 cut(s) 311
AlwI GGATC 3 cut(s) 252, 584, 638
AlwNI CAGNNNCTG 1 cut(s) 339
AoxI GGCC 2 cut(s) 179, 659
ApaLI GTGCAC 1 cut(s) 311
ApoI RAATTY 1 cut(s) 856
Asp700I GAANNNNTTC 1 cut(s) 265
Asp718I GGTACC 1 cut(s) 771
AsuHPI GGTGA 2 cut(s) 136, 284
BaeGI GKGCMC 2 cut(s) 315, 892
BanI GGYRCC 1 cut(s) 771
BanII GRGCYC 1 cut(s) 466
BbsI GAAGAC 1 cut(s) 469
Bbv12I GWGCWC 1 cut(s) 315
BccI CCATC 2 cut(s) 762, 853
BceAI ACGGC 1 cut(s) 584
BcgI CGANNNNNNTGC 4 cut(s) 146, 180, 686, 720
BciT130I CCWGG 2 cut(s) 90, 516
BcoDI GTCTC 2 cut(s) 179, 698
Bme1390I CCNGG 2 cut(s) 90, 516
BmiI GGNNCC 2 cut(s) 321, 773
BmrFI CCNGG 2 cut(s) 90, 516
BmrI ACTGGG 1 cut(s) 302
BmsI GCATC 1 cut(s) 688
BmuI ACTGGG 1 cut(s) 302
BpiI GAAGAC 1 cut(s) 469
BpmI CTGGAG 1 cut(s) 397
BpuEI CTTGAG 1 cut(s) 341
BsaAI YACGTR 1 cut(s) 73
BsaI GGTCTC 1 cut(s) 179
BsaJI CCNNGG 1 cut(s) 600
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 768
Bse1I ACTGG 2 cut(s) 308, 688
Bse3DI GCAATG 1 cut(s) 219
BseBI CCWGG 2 cut(s) 90, 516
BseDI CCNNGG 1 cut(s) 600
BseGI GGATG 1 cut(s) 511
BseLI CCNNNNNNNGG 2 cut(s) 214, 768
BseMI GCAATG 1 cut(s) 219
BseMII CTCAG 1 cut(s) 174
BseNI ACTGG 2 cut(s) 308, 688
BseRI GAGGAG 2 cut(s) 567, 842
BseSI GKGCMC 2 cut(s) 315, 892
BshFI GGCC 2 cut(s) 181, 661
BshNI GGYRCC 1 cut(s) 771
BsiHKAI GWGCWC 1 cut(s) 315
BsiSI CCGG 1 cut(s) 560
BslI CCNNNNNNNGG 2 cut(s) 214, 768
BsmAI GTCTC 2 cut(s) 179, 698
BsnI GGCC 2 cut(s) 181, 661
Bso31I GGTCTC 1 cut(s) 179
Bsp1286I GDGCHC 3 cut(s) 315, 466, 892
Bsp143I GATC 4 cut(s) 244, 589, 630, 785
BspACI CCGC 1 cut(s) 324
BspANI GGCC 2 cut(s) 181, 661
BspCNI CTCAG 1 cut(s) 175
BspLI GGNNCC 2 cut(s) 321, 773
BspPI GGATC 3 cut(s) 252, 584, 638
BspT107I GGYRCC 1 cut(s) 771
BspTNI GGTCTC 1 cut(s) 179
BsrDI GCAATG 1 cut(s) 219
BsrI ACTGG 2 cut(s) 308, 688
BssECI CCNNGG 1 cut(s) 600
BssMI GATC 4 cut(s) 244, 589, 630, 785
Bst2UI CCWGG 2 cut(s) 90, 516
Bst4CI ACNGT 5 cut(s) 118, 550, 713, 749, 913
BstBAI YACGTR 1 cut(s) 73
BstDEI CTNAG 1 cut(s) 183
BstDSI CCRYGG 1 cut(s) 600
BstF5I GGATG 1 cut(s) 511
BstKTI GATC 4 cut(s) 247, 592, 633, 788
BstMAI GTCTC 2 cut(s) 179, 698
BstMBI GATC 4 cut(s) 244, 589, 630, 785
BstMWI GCNNNNNNNGC 2 cut(s) 658, 887
BstNI CCWGG 2 cut(s) 90, 516
BstSCI CCNGG 2 cut(s) 88, 514
BstSLI GKGCMC 2 cut(s) 315, 892
BstSNI TACGTA 1 cut(s) 73
BstV2I GAAGAC 1 cut(s) 469
BsuRI GGCC 2 cut(s) 181, 661
BtgI CCRYGG 1 cut(s) 600
BtsCI GGATG 1 cut(s) 511
BtsIMutI CAGTG 4 cut(s) 315, 640, 681, 918
CaiI CAGNNNCTG 1 cut(s) 339
CseI GACGC 1 cut(s) 416
Csp6I GTAC 5 cut(s) 74, 225, 286, 304, 772
CspCI CAANNNNNGTGG 2 cut(s) 582, 617
CviAII CATG 4 cut(s) 289, 776, 893, 924
CviJI RGCY 9 cut(s) 143, 181, 257, 320, 392, 464, 575, 661, 881
CviKI_1 RGCY 9 cut(s) 143, 181, 257, 320, 392, 464, 575, 661, 881
CviQI GTAC 5 cut(s) 74, 225, 286, 304, 772
DdeI CTNAG 1 cut(s) 183
DpnI GATC 4 cut(s) 246, 591, 632, 787
DpnII GATC 4 cut(s) 244, 589, 630, 785
DraIII CACNNNGTG 1 cut(s) 913
DrdI GACNNNNNNGTC 1 cut(s) 298
DseDI GACNNNNNNGTC 1 cut(s) 298
EaeI YGGCCR 1 cut(s) 659
Eco105I TACGTA 1 cut(s) 73
Eco147I AGGCCT 1 cut(s) 181
Eco24I GRGCYC 1 cut(s) 466
Eco31I GGTCTC 1 cut(s) 179
Eco57I CTGAAG 1 cut(s) 165
EcoRII CCWGG 2 cut(s) 88, 514
EcoT22I ATGCAT 1 cut(s) 898
EcoT38I GRGCYC 1 cut(s) 466
FaeI CATG 4 cut(s) 292, 779, 896, 927
FalI AAGNNNNNCTT 4 cut(s) 564, 596, 725, 757
FatI CATG 4 cut(s) 288, 775, 892, 923
FauI CCCGC 1 cut(s) 331
FauNDI CATATG 3 cut(s) 430, 721, 898
FokI GGATG 1 cut(s) 498
FriOI GRGCYC 1 cut(s) 466
GsuI CTGGAG 1 cut(s) 397
HaeIII GGCC 2 cut(s) 181, 661
HapII CCGG 1 cut(s) 560
HgaI GACGC 1 cut(s) 416
Hin1II CATG 4 cut(s) 292, 779, 896, 927
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HinfI GANTC 5 cut(s) 158, 204, 266, 275, 904
HpaII CCGG 1 cut(s) 560
HphI GGTGA 2 cut(s) 136, 284
Hpy166II GTNNAC 5 cut(s) 76, 103, 136, 313, 604
Hpy188III TCNNGA 4 cut(s) 358, 376, 493, 695
Hpy8I GTNNAC 5 cut(s) 76, 103, 136, 313, 604
Hpy99I CGWCG 1 cut(s) 304
HpyAV CCTTC 4 cut(s) 121, 215, 577, 751
HpyCH4III ACNGT 5 cut(s) 118, 550, 713, 749, 913
HpyCH4IV ACGT 2 cut(s) 72, 474
HpyCH4V TGCA 6 cut(s) 212, 230, 313, 428, 796, 896
HpyF10VI GCNNNNNNNGC 2 cut(s) 658, 887
HpyF3I CTNAG 1 cut(s) 183
HpySE526I ACGT 2 cut(s) 72, 474
Hsp92II CATG 4 cut(s) 292, 779, 896, 927
KpnI GGTACC 1 cut(s) 775
Kzo9I GATC 4 cut(s) 244, 589, 630, 785
LmnI GCTCC 4 cut(s) 325, 378, 580, 878
LweI GCATC 1 cut(s) 688
MaeII ACGT 2 cut(s) 72, 474
MaeIII GTNAC 2 cut(s) 507, 620
MalI GATC 4 cut(s) 246, 591, 632, 787
MboI GATC 4 cut(s) 244, 589, 630, 785
MboII GAAGA 3 cut(s) 158, 413, 469
MfeI CAATTG 2 cut(s) 594, 813
MhlI GDGCHC 3 cut(s) 315, 466, 892
MluCI AATT 5 cut(s) 45, 594, 813, 856, 917
MlyI GAGTC 1 cut(s) 198
MmeI TCCRAC 2 cut(s) 532, 856
MnlI CCTC 5 cut(s) 57, 171, 588, 792, 820
Mph1103I ATGCAT 1 cut(s) 898
MroXI GAANNNNTTC 1 cut(s) 265
MseI TTAA 2 cut(s) 120, 395
MslI CAYNNNNRTG 1 cut(s) 897
MspI CCGG 1 cut(s) 560
MspR9I CCNGG 2 cut(s) 90, 516
MunI CAATTG 2 cut(s) 594, 813
MvaI CCWGG 2 cut(s) 90, 516
MwoI GCNNNNNNNGC 2 cut(s) 658, 887
NdeI CATATG 3 cut(s) 430, 721, 898
NdeII GATC 4 cut(s) 244, 589, 630, 785
NlaIII CATG 4 cut(s) 292, 779, 896, 927
NlaIV GGNNCC 2 cut(s) 321, 773
NmeAIII GCCGAG 1 cut(s) 687
NmuCI GTSAC 1 cut(s) 620
NsiI ATGCAT 1 cut(s) 898
PceI AGGCCT 1 cut(s) 181
PdmI GAANNNNTTC 1 cut(s) 265
PfeI GAWTC 4 cut(s) 158, 266, 275, 904
PflFI GACNNNGTC 1 cut(s) 295
PfoI TCCNGGA 1 cut(s) 514
PleI GAGTC 1 cut(s) 198
PpsI GAGTC 1 cut(s) 198
Ppu21I YACGTR 1 cut(s) 73
Psp6I CCWGG 2 cut(s) 88, 514
PspGI CCWGG 2 cut(s) 88, 514
PspN4I GGNNCC 2 cut(s) 321, 773
PstNI CAGNNNCTG 1 cut(s) 339
PsyI GACNNNGTC 1 cut(s) 295
RsaI GTAC 5 cut(s) 75, 226, 287, 305, 773
RsaNI GTAC 5 cut(s) 74, 225, 286, 304, 772
RseI CAYNNNNRTG 1 cut(s) 897
SaqAI TTAA 2 cut(s) 120, 395
Sau3AI GATC 4 cut(s) 244, 589, 630, 785
SchI GAGTC 1 cut(s) 198
ScrFI CCNGG 2 cut(s) 90, 516
SduI GDGCHC 3 cut(s) 315, 466, 892
SfaNI GCATC 1 cut(s) 688
SmiMI CAYNNNNRTG 1 cut(s) 897
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
SnaBI TACGTA 1 cut(s) 73
Sse9I AATT 5 cut(s) 45, 594, 813, 856, 917
SseBI AGGCCT 1 cut(s) 181
SsiI CCGC 1 cut(s) 324
StuI AGGCCT 1 cut(s) 181
StyD4I CCNGG 2 cut(s) 88, 514
TaaI ACNGT 5 cut(s) 118, 550, 713, 749, 913
TaiI ACGT 2 cut(s) 75, 477
TaqI TCGA 4 cut(s) 62, 492, 696, 784
TasI AATT 5 cut(s) 45, 594, 813, 856, 917
TfiI GAWTC 4 cut(s) 158, 266, 275, 904
Tru1I TTAA 2 cut(s) 120, 395
Tru9I TTAA 2 cut(s) 120, 395
TscAI CASTG 4 cut(s) 315, 640, 688, 918
TseFI GTSAC 1 cut(s) 620
Tsp45I GTSAC 1 cut(s) 620
TspDTI ATGAA 5 cut(s) 165, 341, 381, 522, 708
TspRI CASTG 4 cut(s) 315, 640, 688, 918
Tth111I GACNNNGTC 1 cut(s) 295
VneI GTGCAC 1 cut(s) 311
XapI RAATTY 1 cut(s) 856
XcmI CCANNNNNNNNNTGG 1 cut(s) 86
XmnI GAANNNNTTC 1 cut(s) 265
Zsp2I ATGCAT 1 cut(s) 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.