FvH4_1g09190

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001959
Physical Location & Seq
Reverse (-)
4895374 .. 4900540
5167 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g09190.t2

Sequence Viewer

Length: 2484 bp
ATGCCTCCCTCCAAAAACTTCACGCTTGCCCTTTTGTTTTGTTTTTCCTTGATTCTTCTCAATTCATATGCAAGTGCCATCAAAGTTTCTCATGACGGAAGATCCATCACCATCGATGGCAAGCCCAGAATTCTGCTGTCTGGTTCCATTCACTATCCTCGAAGCACACCAGAAATGTGGCCCGAATTGATTAAGAAATCAAAGGAGGGTGGATTAGACGCAATCGAGACCTATGTATTCTGGAATGCTCATGCACCAGTGCGTCGCCAATATGATTTTACTGGCAACCTAGACCTCATTAAGTTCATCAAAACCGCTCAAGATGCTGGACTATACGTCGTTCTTCGAATCGGTCCGTATGTTTGTGCTGAATGGAACTATGGAGGACTTCCTGTTTGGTTGCACAATCTTCCCGGCTGTCAAATTCGAACTGCAAATGATGTCTTCATGAATGAGATGAAAAATTTCACTACCATGATTGTTGATATGGCCAAAAGAGAGAAGCTGTTTGCATCTCAAGGAGGCCCTATTATTATGGCTCAGATTGAAAATGAATATGGAGATGTGGAATGGTGGTATGGAGCTGCTGGTAAAGTATATCGTGACTGGTGTGCAAATTTTGCTGAATCTCTTAATGTTGGTGTTCCATGGATTATGTGTCAACAAAACGATGCCCCAGATCCAATGATAAACACTTGCAATGGCTACTACTGCGACCAGTTTACACCCAAACCCAATTATCCAAAAATGTGGACTGAAAATTGGACAGGATGGTTTAAGAGCTGGGGTGCCTCAGATCCATACAGGACAGCTGAGGATCTTGCTTTCGCAGTTGCAAAATTTTTCCAATTTGGAGGCACATTTCAGAACTATTACATGTATCATGGTGGTACAAACTTTGGTCGAGTAGCAGCTGGATACATCACCACTTCATATGATTACAATGCTCCTCTTGATGAATATGGTGCTTTGAATCAACCAAAATGGGGGCATTTGAAGCAACTCCATGAGACTTTGAAATCCATGGAGTACACTCTTACGCATGGAAATATTACCGCCACGGATTTGGGTAACAATGTTACGACCACTGTGTATGCAACAGAAGAGTCGTCGAGCTGCTTTTTGTGCAATTCAGATTACAAACTTGATGCAAACATCAGTTTCCGGGGAACCAATTACTTTGTTCCGGCGTGGTCTGTTAGTATTCTTCCTGATTGCAAAACTGAAGCATATAACACTGCTAAGGTGAACACCCAGACAACCGTAAAAGTAAAAAGAAATAACAAAGCTGAAGATGAACCTCAATCTCTTCATTGGACATGGAGGCCTGAGAACATAGATGAGACTAAGATACTTGGAAAAGGTGAAGTTGTGGTAAATCACCTTATGGAGCAAAAAGAGGCTGCAAATGATGAAAGTGATTACCTTTGGTACATGACAAGTGTTGATCTCAAGAAAGATGATCCAATTTGGGGTAAGAACAACATTACATTAAGGATCAATGGAACTGGTTGCATTCTTCATGCTTATGTGAATGGAGAGCATATCGGATCTGACCAAGCTGCTCGAAATTTTGAGAGAAACGTCACACTAAAGGATGGAAGAAACATTATTTCCCTGTTGAGTGCCACAACTGGATTTTCGAATTATGGAGGTGGATATGATTTGCAACCTGCTGGAATACTTGGTCCTGTTGTTTTGGTTGGACAGAATGGTGATGATGAATCTGTTATCAAGGATTTGTCAGGTCACAAATGGTCATACAAGGTTGGATTACATGGCCTTACAAACAAGCTATTTAGCTCCGAGAAGGCAACGAAATGGTCGTCTGAAAATTTACCAGTAAACACCACGATGACATGGTACAAGACAACTTTCAAAGCTCCTCTGGGAACTGACCCAGTTGTGGTGGATTTACAAGGGTTGGGGAAGGGTAATGCTTGGGTGAATGGTCATAGTATAGGTCGATATTGGCCAAGTTTTTTGGCTAAGACCGACGGCTGCAGCGATGAATGCGACTATCGTGGTAAATACGAAAGTAAAAAATGTAATTCCAAGTGTGGCGAACCTACTCAAAGATGGTATCATATTCCACGATCTTTCATGCGAGACGATGATGAGAACACATTGGTGTTGTTTGAGGAATTTGGTGGCGATCCATCTCTTGTAAAGTTCCAGACCATCGCAGTCGGAAGTGTTTGTGCCAATGCATATGAGAATAACACCTTGGAATTGGCATGCGATGGTCGCTCGATTTCAGCAATCAAATTTGCTAGCTTTGGCAACCCTCAAGGTACCTGTGGATCATTCAAGAACGGAACATCATTCACGAAAGGCACCTGTGATGGCTCAAATGCACTATCCATCATTCAAAAGGAATGTGTCGGTAAAGAAAAATGCTCGATTGATGTCTCCGGAGACAAATTTGACTCGACAAATTGTGGGGATATTGTGAAGAGGCTTGCAGTAGAGGCAGTTTGCTAG

Protein Analysis

828

Amino Acids

92.29

Weight (kDa)

5.82

Isoelectric Point (pI)

31.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 1 - 280 2.9e-103 Glycosyl hydrolases family 35
GHD PF17834 304 - 358 4.1e-23 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 561 - 646 1.9e-23 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 688 - 769 3.3e-17 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1681
Acc65I GGTACC 1 cut(s) 2294
AccB1I GGYRCC 3 cut(s) 788, 2294, 2336
AccBSI CCGCTC 1 cut(s) 317
AccIII TCCGGA 1 cut(s) 2414
AciI CCGC 2 cut(s) 315, 1056
AclWI GGATC 9 cut(s) 96, 674, 791, 825, 1457, 1505, 1558, 2150, 2311
AcoI YGGCCR 2 cut(s) 489, 1973
AcuI CTGAAG 2 cut(s) 1245, 1311
AfaI GTAC 5 cut(s) 892, 1031, 1433, 1865, 2296
AfiI CCNNNNNNNGG 3 cut(s) 986, 1472, 1906
AflIII ACRYGT 1 cut(s) 876
AgsI TTSAA 7 cut(s) 548, 973, 997, 1018, 1879, 2311, 2372
AhdI GACNNNNNGTC 1 cut(s) 335
AjuI GAANNNNNNNTTGG 2 cut(s) 2210, 2242
AleI CACNNNNGTG 1 cut(s) 2129
Alw26I GTCTC 6 cut(s) 221, 1004, 1337, 2103, 2412, 2416
AlwI GGATC 9 cut(s) 96, 674, 791, 825, 1457, 1505, 1558, 2150, 2311
Aor13HI TCCGGA 1 cut(s) 2414
AoxI GGCC 6 cut(s) 179, 489, 523, 1325, 1780, 1973
ApeKI GCWGC 7 cut(s) 584, 911, 1116, 1403, 1562, 2001, 2004
ArsI GACNNNNNNTTYG 4 cut(s) 378, 410, 1811, 1843
Asp700I GAANNNNTTC 1 cut(s) 464
Asp718I GGTACC 1 cut(s) 2294
AspS9I GGNCC 4 cut(s) 180, 353, 524, 1688
AsuC2I CCSGG 2 cut(s) 414, 1166
AsuHPI GGTGA 7 cut(s) 100, 916, 1258, 1373, 1376, 1727, 1957
AsuII TTCGAA 3 cut(s) 346, 427, 1643
AsuNHI GCTAGC 1 cut(s) 2273
AvaII GGWCC 2 cut(s) 353, 1688
BalI TGGCCA 2 cut(s) 491, 1975
BanI GGYRCC 3 cut(s) 788, 2294, 2336
BbsI GAAGAC 1 cut(s) 436
BbvCI CCTCAGC 1 cut(s) 813
BbvI GCAGC 7 cut(s) 571, 923, 1103, 1390, 1549, 1988, 2016
BceAI ACGGC 1 cut(s) 2014
BciVI GTATCC 1 cut(s) 911
BcnI CCSGG 2 cut(s) 414, 1166
BcoDI GTCTC 6 cut(s) 221, 1004, 1337, 2103, 2412, 2416
BfaI CTAG 3 cut(s) 290, 2274, 2482
BfmI CTRYAG 1 cut(s) 2002
BfuAI ACCTGC 1 cut(s) 1681
BfuI GTATCC 1 cut(s) 911
BisI GCNGC 7 cut(s) 585, 912, 1117, 1404, 1563, 2002, 2005
BlsI GCNGC 7 cut(s) 586, 913, 1118, 1405, 1564, 2003, 2006
Bme1390I CCNGG 2 cut(s) 414, 1166
Bme18I GGWCC 2 cut(s) 353, 1688
BmeRI GACNNNNNGTC 1 cut(s) 335
BmgT120I GGNCC 4 cut(s) 180, 353, 524, 1688
BmiI GGNNCC 5 cut(s) 145, 790, 1171, 2296, 2338
BmrFI CCNGG 2 cut(s) 414, 1166
BmrI ACTGGG 1 cut(s) 1895
BmsI GCATC 4 cut(s) 313, 521, 661, 1138
BmtI GCTAGC 1 cut(s) 2277
BmuI ACTGGG 1 cut(s) 1895
BpiI GAAGAC 1 cut(s) 436
Bpu10I CCTNAGC 2 cut(s) 813, 1242
Bpu14I TTCGAA 3 cut(s) 346, 427, 1643
BpuEI CTTGAG 4 cut(s) 303, 501, 1436, 2274
BpuMI CCSGG 2 cut(s) 414, 1166
Bsa29I ATCGAT 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 221
BsaJI CCNNGG 5 cut(s) 647, 1023, 1059, 1165, 2226
BsaWI WCCGGW 1 cut(s) 2414
Bsc4I CCNNNNNNNGG 3 cut(s) 986, 1472, 1906
Bse1I ACTGG 8 cut(s) 257, 286, 611, 718, 1513, 1639, 1841, 1901
Bse3DI GCAATG 1 cut(s) 706
BseAI TCCGGA 1 cut(s) 2414
BseCI ATCGAT 1 cut(s) 114
BseDI CCNNGG 5 cut(s) 647, 1023, 1059, 1165, 2226
BseGI GGATG 2 cut(s) 776, 1603
BseLI CCNNNNNNNGG 3 cut(s) 986, 1472, 1906
BseMI GCAATG 1 cut(s) 706
BseMII CTCAG 4 cut(s) 554, 804, 807, 1320
BseNI ACTGG 8 cut(s) 257, 286, 611, 718, 1513, 1639, 1841, 1901
BseRI GAGGAG 2 cut(s) 939, 1875
BseXI GCAGC 7 cut(s) 571, 923, 1103, 1390, 1549, 1988, 2016
BseYI CCCAGC 1 cut(s) 783
BshFI GGCC 6 cut(s) 181, 491, 525, 1327, 1782, 1975
BshNI GGYRCC 3 cut(s) 788, 2294, 2336
BshVI ATCGAT 1 cut(s) 114
BsiSI CCGG 4 cut(s) 414, 1165, 1187, 2415
BslI CCNNNNNNNGG 3 cut(s) 986, 1472, 1906
BsmAI GTCTC 6 cut(s) 221, 1004, 1337, 2103, 2412, 2416
BsmBI CGTCTC 1 cut(s) 2103
BsmI GAATGC 3 cut(s) 250, 1515, 2018
BsnI GGCC 6 cut(s) 181, 491, 525, 1327, 1782, 1975
Bso31I GGTCTC 1 cut(s) 221
Bsp119I TTCGAA 3 cut(s) 346, 427, 1643
Bsp13I TCCGGA 1 cut(s) 2414
Bsp19I CCATGG 2 cut(s) 647, 1023
BspACI CCGC 2 cut(s) 315, 1056
BspANI GGCC 6 cut(s) 181, 491, 525, 1327, 1782, 1975
BspCNI CTCAG 4 cut(s) 553, 805, 806, 1321
BspDI ATCGAT 1 cut(s) 114
BspEI TCCGGA 1 cut(s) 2414
BspHI TCATGA 2 cut(s) 91, 447
BspLI GGNNCC 5 cut(s) 145, 790, 1171, 2296, 2338
BspMAI CTGCAG 1 cut(s) 2006
BspMI ACCTGC 1 cut(s) 1681
BspOI GCTAGC 1 cut(s) 2277
BspPI GGATC 9 cut(s) 96, 674, 791, 825, 1457, 1505, 1558, 2150, 2311
BspT104I TTCGAA 3 cut(s) 346, 427, 1643
BspT107I GGYRCC 3 cut(s) 788, 2294, 2336
BspTNI GGTCTC 1 cut(s) 221
BsrBI CCGCTC 1 cut(s) 317
BsrDI GCAATG 1 cut(s) 706
BsrI ACTGG 8 cut(s) 257, 286, 611, 718, 1513, 1639, 1841, 1901
BssECI CCNNGG 5 cut(s) 647, 1023, 1059, 1165, 2226
BssT1I CCWWGG 3 cut(s) 647, 1023, 2226
Bst4CI ACNGT 2 cut(s) 1090, 1264
Bst6I CTCTTC 3 cut(s) 1098, 1314, 2450
BstAPI GCANNNNNTGC 1 cut(s) 620
BstBI TTCGAA 3 cut(s) 346, 427, 1643
BstC8I GCNNGC 5 cut(s) 27, 122, 2239, 2275, 2463
BstDEI CTNAG 7 cut(s) 540, 793, 813, 1242, 1329, 1347, 1989
BstDSI CCRYGG 3 cut(s) 647, 1023, 1059
BstF5I GGATG 2 cut(s) 776, 1603
BstMAI GTCTC 6 cut(s) 221, 1004, 1337, 2103, 2412, 2416
BstMWI GCNNNNNNNGC 8 cut(s) 323, 620, 711, 997, 1125, 2013, 2247, 2471
BstNSI RCATGY 2 cut(s) 880, 2241
BstSCI CCNGG 2 cut(s) 412, 1164
BstSFI CTRYAG 1 cut(s) 2002
BstV1I GCAGC 7 cut(s) 571, 923, 1103, 1390, 1549, 1988, 2016
BstV2I GAAGAC 1 cut(s) 436
BstX2I RGATCY 5 cut(s) 101, 679, 796, 817, 1550
BstXI CCANNNNNNTGG 3 cut(s) 177, 750, 1066
BstYI RGATCY 5 cut(s) 101, 679, 796, 817, 1550
Bsu15I ATCGAT 1 cut(s) 114
BsuI GTATCC 1 cut(s) 911
BsuRI GGCC 6 cut(s) 181, 491, 525, 1327, 1782, 1975
BsuTUI ATCGAT 1 cut(s) 114
BtgI CCRYGG 3 cut(s) 647, 1023, 1059
BtgZI GCGATG 3 cut(s) 2022, 2167, 2256
BtsCI GGATG 2 cut(s) 776, 1603
BtsI GCAGTG 1 cut(s) 1236
BtsIMutI CAGTG 3 cut(s) 264, 1086, 1236
BveI ACCTGC 1 cut(s) 1681
Cac8I GCNNGC 5 cut(s) 27, 122, 2239, 2275, 2463
CciI TCATGA 2 cut(s) 91, 447
Cfr13I GGNCC 4 cut(s) 180, 353, 524, 1688
ClaI ATCGAT 1 cut(s) 114
CpoI CGGWCCG 1 cut(s) 353
CseI GACGC 2 cut(s) 227, 251
Csp6I GTAC 5 cut(s) 891, 1030, 1432, 1864, 2295
CspI CGGWCCG 1 cut(s) 353
CviQI GTAC 5 cut(s) 891, 1030, 1432, 1864, 2295
DdeI CTNAG 7 cut(s) 540, 793, 813, 1242, 1329, 1347, 1989
DriI GACNNNNNGTC 1 cut(s) 335
EaeI YGGCCR 2 cut(s) 489, 1973
Eam1104I CTCTTC 3 cut(s) 1098, 1314, 2450
Eam1105I GACNNNNNGTC 1 cut(s) 335
EarI CTCTTC 3 cut(s) 1098, 1314, 2450
Eco130I CCWWGG 3 cut(s) 647, 1023, 2226
Eco147I AGGCCT 1 cut(s) 1327
Eco31I GGTCTC 1 cut(s) 221
Eco47I GGWCC 2 cut(s) 353, 1688
Eco57I CTGAAG 2 cut(s) 1245, 1311
EcoO109I RGGNCCY 1 cut(s) 524
EcoRI GAATTC 1 cut(s) 129
EcoT14I CCWWGG 3 cut(s) 647, 1023, 2226
EcoT22I ATGCAT 1 cut(s) 2212
ErhI CCWWGG 3 cut(s) 647, 1023, 2226
Esp3I CGTCTC 1 cut(s) 2103
FauNDI CATATG 3 cut(s) 67, 934, 2212
Fnu4HI GCNGC 7 cut(s) 585, 912, 1117, 1404, 1563, 2002, 2005
FokI GGATG 2 cut(s) 783, 1610
Fsp4HI GCNGC 7 cut(s) 585, 912, 1117, 1404, 1563, 2002, 2005
FspBI CTAG 3 cut(s) 290, 2274, 2482
GluI GCNGC 7 cut(s) 585, 912, 1117, 1404, 1563, 2002, 2005
GsaI CCCAGC 1 cut(s) 787
HaeIII GGCC 6 cut(s) 181, 491, 525, 1327, 1782, 1975
HapII CCGG 4 cut(s) 414, 1165, 1187, 2415
HgaI GACGC 2 cut(s) 227, 251
HincII GTYRAC 1 cut(s) 662
HindII GTYRAC 1 cut(s) 662
HinfI GANTC 7 cut(s) 52, 348, 626, 973, 1106, 1724, 2429
HpaII CCGG 4 cut(s) 414, 1165, 1187, 2415
HphI GGTGA 7 cut(s) 100, 916, 1258, 1373, 1376, 1727, 1957
Hpy166II GTNNAC 6 cut(s) 662, 723, 753, 1032, 1249, 1846
Hpy188I TCNGA 9 cut(s) 543, 796, 867, 1135, 1550, 1555, 1807, 1831, 2192
Hpy8I GTNNAC 6 cut(s) 662, 723, 753, 1032, 1249, 1846
Hpy99I CGWCG 4 cut(s) 267, 341, 1114, 2000
HpyAV CCTTC 2 cut(s) 1804, 1924
HpyCH4III ACNGT 2 cut(s) 1090, 1264
HpyCH4IV ACGT 2 cut(s) 336, 1584
HpyF10VI GCNNNNNNNGC 8 cut(s) 323, 620, 711, 997, 1125, 2013, 2247, 2471
HpyF3I CTNAG 7 cut(s) 540, 793, 813, 1242, 1329, 1347, 1989
HpySE526I ACGT 2 cut(s) 336, 1584
Kpn2I TCCGGA 1 cut(s) 2414
KpnI GGTACC 1 cut(s) 2298
LmnI GCTCC 5 cut(s) 581, 952, 1390, 1808, 1888
Lsp1109I GCAGC 7 cut(s) 571, 923, 1103, 1390, 1549, 1988, 2016
LweI GCATC 4 cut(s) 313, 521, 661, 1138
MaeI CTAG 3 cut(s) 290, 2274, 2482
MaeII ACGT 2 cut(s) 336, 1584
MaeIII GTNAC 5 cut(s) 602, 1070, 1078, 1585, 1748
MbiI CCGCTC 1 cut(s) 317
MflI RGATCY 5 cut(s) 101, 679, 796, 817, 1550
MlsI TGGCCA 2 cut(s) 491, 1975
MluNI TGGCCA 2 cut(s) 491, 1975
MlyI GAGTC 2 cut(s) 1115, 2423
MmeI TCCRAC 3 cut(s) 1684, 1750, 2170
Mox20I TGGCCA 2 cut(s) 491, 1975
Mph1103I ATGCAT 1 cut(s) 2212
MroI TCCGGA 1 cut(s) 2414
MroXI GAANNNNTTC 1 cut(s) 464
MscI TGGCCA 2 cut(s) 491, 1975
MseI TTAA 5 cut(s) 192, 300, 633, 777, 1493
MslI CAYNNNNRTG 4 cut(s) 473, 1527, 1853, 2129
Msp20I TGGCCA 2 cut(s) 491, 1975
MspA1I CMGCKG 2 cut(s) 812, 914
MspI CCGG 4 cut(s) 414, 1165, 1187, 2415
MspR9I CCNGG 2 cut(s) 414, 1166
Mva1269I GAATGC 3 cut(s) 250, 1515, 2018
MwoI GCNNNNNNNGC 8 cut(s) 323, 620, 711, 997, 1125, 2013, 2247, 2471
NciI CCSGG 2 cut(s) 414, 1166
NcoI CCATGG 2 cut(s) 647, 1023
NdeI CATATG 3 cut(s) 67, 934, 2212
NheI GCTAGC 1 cut(s) 2273
NlaIV GGNNCC 5 cut(s) 145, 790, 1171, 2296, 2338
NmuCI GTSAC 3 cut(s) 602, 1585, 1748
NsiI ATGCAT 1 cut(s) 2212
NspI RCATGY 2 cut(s) 880, 2241
NspV TTCGAA 3 cut(s) 346, 427, 1643
OliI CACNNNNGTG 1 cut(s) 2129
PaeI GCATGC 1 cut(s) 2241
PagI TCATGA 2 cut(s) 91, 447
PceI AGGCCT 1 cut(s) 1327
PciI ACATGT 1 cut(s) 876
PcsI WCGNNNNNNNCGW 2 cut(s) 1823, 2004
PctI GAATGC 3 cut(s) 250, 1515, 2018
PdmI GAANNNNTTC 1 cut(s) 464
PfeI GAWTC 5 cut(s) 52, 348, 626, 973, 1724
PkrI GCNGC 7 cut(s) 586, 913, 1118, 1405, 1564, 2003, 2006
PleI GAGTC 2 cut(s) 1114, 2423
PpsI GAGTC 2 cut(s) 1114, 2423
PscI ACATGT 1 cut(s) 876
PspFI CCCAGC 1 cut(s) 783
PspN4I GGNNCC 5 cut(s) 145, 790, 1171, 2296, 2338
PspPI GGNCC 4 cut(s) 180, 353, 524, 1688
PstI CTGCAG 1 cut(s) 2006
PsuI RGATCY 5 cut(s) 101, 679, 796, 817, 1550
PvuII CAGCTG 2 cut(s) 812, 914
RsaI GTAC 5 cut(s) 892, 1031, 1433, 1865, 2296
RsaNI GTAC 5 cut(s) 891, 1030, 1432, 1864, 2295
RseI CAYNNNNRTG 4 cut(s) 473, 1527, 1853, 2129
Rsr2I CGGWCCG 1 cut(s) 353
RsrII CGGWCCG 1 cut(s) 353
SaqAI TTAA 5 cut(s) 192, 300, 633, 777, 1493
SatI GCNGC 7 cut(s) 585, 912, 1117, 1404, 1563, 2002, 2005
Sau96I GGNCC 4 cut(s) 180, 353, 524, 1688
SchI GAGTC 2 cut(s) 1115, 2423
ScrFI CCNGG 2 cut(s) 414, 1166
SfaNI GCATC 4 cut(s) 313, 521, 661, 1138
SfcI CTRYAG 1 cut(s) 2002
SfuI TTCGAA 3 cut(s) 346, 427, 1643
SinI GGWCC 2 cut(s) 353, 1688
SmiMI CAYNNNNRTG 4 cut(s) 473, 1527, 1853, 2129
SmlI CTYRAG 4 cut(s) 318, 516, 1451, 2289
SmoI CTYRAG 4 cut(s) 318, 516, 1451, 2289
SphI GCATGC 1 cut(s) 2241
SseBI AGGCCT 1 cut(s) 1327
SsiI CCGC 2 cut(s) 315, 1056
SspI AATATT 1 cut(s) 1051
SspMI CTAG 3 cut(s) 290, 2274, 2482
StuI AGGCCT 1 cut(s) 1327
StyD4I CCNGG 2 cut(s) 412, 1164
StyI CCWWGG 3 cut(s) 647, 1023, 2226
TaaI ACNGT 2 cut(s) 1090, 1264
TaiI ACGT 2 cut(s) 339, 1587
TaqII GACCGA 2 cut(s) 341, 2009
TatI WGTACW 1 cut(s) 1029
TfiI GAWTC 5 cut(s) 52, 348, 626, 973, 1724
Tru1I TTAA 5 cut(s) 192, 300, 633, 777, 1493
Tru9I TTAA 5 cut(s) 192, 300, 633, 777, 1493
TscAI CASTG 3 cut(s) 264, 1093, 1243
TseFI GTSAC 3 cut(s) 602, 1585, 1748
TseI GCWGC 7 cut(s) 584, 911, 1116, 1403, 1562, 2001, 2004
Tsp45I GTSAC 3 cut(s) 602, 1585, 1748
TspGWI ACGGA 4 cut(s) 111, 345, 1076, 2331
TspRI CASTG 3 cut(s) 264, 1093, 1243
VpaK11BI GGWCC 2 cut(s) 353, 1688
XceI RCATGY 2 cut(s) 880, 2241
XmnI GAANNNNTTC 1 cut(s) 464
XspI CTAG 3 cut(s) 290, 2274, 2482
Zsp2I ATGCAT 1 cut(s) 2212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.