Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001959

Physical Location & Seq

Genomic Coordinates Reverse (-)

17876698 .. 17880733

Gene Structure 4036 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1220900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2256 bp

ATGGCTTTTTCCAACTCTTTTAGTTTTGCGTTTTGTTTCTGTTTTTCTGTGATCCTTTTAAGCTTTTATGCTAATGCTACTGATGTTTCTCATGATGGGAGAGCCATCACAATCGATGGTAAAAGGCGACTGTTGCTTTCGGGTTCAATCCACTATCCTCGAAGCACACCACAAATGTGGCCAGACTTGATTAAGAAGTCCAAGGAGGGTGGACTAGACACAATTGAGACTTATGTCTTCTGGAATGTGCATGAGCCAGTTCGTCGTCAGTATGATTTTACTGGGAATCTTGACCTTGTTAGATTTCTCAAAACAGTTCAAGATGAAGGACTTTATGCTGTTCTTCGAATTGGTCCATATGTTTGTGCCGAATGGAATTATGGAGGACTTCCCGTTTGGCTGCACAACTTGCCTGGCTGTAATGAGATGAAGAATTTTACCACATTGATTGTTGATATGATGAAAAAAGAAAAGCTTTTTGCATCTCAAGGTGGCCCAATTATTATTGCTCAGATTGAAAATGAATACGGAAATGTGCAATCATACTATGGAGATGCTGGTAAAGCATATATGAAACTGGTGTGCAAATTTTGCCGAATCTCTCGACATTGGAGTTCCATGGATTATGTGCCAACAAAGTTTAAGAGTTGGGGAGGCAAAGATCCGCTTAGGACTGCTGAGGATCTTGCTTTCTCTGTTGCAAGATTTTTCCAGCTTGGAGGCACATTTCAGAACTACTACATGTATCATGGTGGAACAAACTTTGGCCGAACAGCAGCTGCGTACGTCACTACTACTTATGATTACGATGCTCCTCTCGACGAATATGGCAACTTGAACCAACCAAAATGGGGGCATTTGAAAGAACTCCATCAGATTTTGAAATCCATGGAGTATACTCTCACACATGGGAATGTTACAACCACAAATTTTGACAATTCTGTTTCAGCCACTGTGTATGCAACAAATGAGTCATCAAGCTGCTTTTTGGGCAATGCAAATAGCACCACTGATGCTACAATCACTTTTCAAGGGAATACATACACCATTCCTGCTTGGTCTGTTAGTATTCTTCCTGATTGCAAAACAGAAGGATATAACACTGCAAAGGTCAACACACAAACAACAGTTAAGGTGAAGGTAGGGAACAAGGCTGAAGATGAACCCGAATCACTTGCTTGGTCGTGGAGGCCCGAGACCATTGATGACACCAGAGTCCTTGCAAAGGGAGAAATTACTGCAAATCAACTTTTGGATCAAAAGGTTGCTGGAGATGATGCTAGTGATTATCTTTGGTACATGACAAGTGTTCATCTCAAGAAAGATGATCCAATTTGGAGTAAGAACATGACTCTAAGGGTCAATGGCACTGGTTTCATTCTTCATGCATATGTTAATGGAGAATATGTTGGTTCTCAATGGGCTCAATACAACGTCTACAACTATGTATTTGAGAAGAGTGTCAAGTTGCATCCTGGAAAGAACATTATTTCATTGTTGAGTGCTACCGTTGGATTTCCGAATTACGGAGGTGGATTTGAAGAGATCAAAACTGGAATTTCTGGTCCAGTTGTACTGGTGGGACAAAATGGTGATGAGATTGTGATCAAGGATCTATCAGCACACAAATGGTCGTACAAGGTTGGACTTCATGGTTTGAAAAACAAGCTTTTTAGCACCGATTCTCGTTACGCTACTAAGTGGTCAACGGAAAACTTACCCATTAACCGGATGCTGACATGGTACAAGGCAACTTTCAAAGCTCCTCTTGGAACTGATCCAGTTGTGGTGGACTTACAAGGTTTGGGAAAGGGTCACGCTTGGATCAATGGCCATAGTATTGGCCGGTATTGGCCGAGTTATTTGTCATATGATACTGATTGTAGTCTCGATGCTTGTGACTACCGTGGTACATATGACAACAACAAGTGTTCCTTCAATTGTGGCAATCCTACCCAAAGATGGTACCACGTTCCTCGATCATTTATGGAAGATGGAGAAAATGCAATTGTGTTGTTTGAGGAGTTTGGTGGCAATCCATCTCTTGTGAATTTCCAAAACCATCCAAGTTGGAACCGTCTGTGCCCATGCATACGAGAAGCACACAGTGGAATTGGCATGCGAAGGTCGTCCCATTTCTGCAATCAAGAATGCGTGGGCAAGGAGAAGTGCTTGATCAATGTTTCTGAGAGCAAATTTGGCTTGACAAACTGCGGTGATGCCGTGAAGAGGCTGGCTGTGGAAGCTGTTTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BetaGal_dom4_5 Gal_Lectin Glyco_hydro_35

Protein Analysis

752

Amino Acids

84.44

Weight (kDa)

6.85

Isoelectric Point (pI)

31.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	36 - 194	1.4e-62	Glycosyl hydrolases family 35
Glyco_hydro_35	PF01301	207 - 292	5.4e-26	Glycosyl hydrolases family 35
GHD	PF17834	317 - 371	4.6e-23	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	579 - 668	8.4e-25	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	705 - 751	6.8e-06	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001959)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31740 AT2G04060 AT5G20710 AT5G20710
fragaria_vesca	FvH4_1g09120 FvH4_1g09190 FvH4_1g09190 FvH4_1g26380
malus_domestica	MD02G1096400.v1.1 MD03G1276000.v1.1 MD15G1220900.v1.1 MD15G1221000.v1.1
prunus_persica	Prupe.7G194500_v2.0.a1
pyrus_communis	pycom02g07640
rosa_chinensis	RchiOBHm_Chr2g0095511 RchiOBHm_Chr2g0095591 RchiOBHm_Chr5g0030701
rosa_laevigata	RLG00000016574 RLG00000016582 RLG00000016583
rosa_multiflora	Rmu_co8462209.1_g000001 Rmu_sc0003238.1_g000025 Rmu_sc0003238.1_g000028 Rmu_sc0004719.1_g000009
rosa_roxburghii	Rroxscaffold_2G00146240
rosa_rugosa	Rorug02G0051800 Rorug02G0052600 Rorug05G0120200
rosa_samantha	Rh2AG099800 Rh2AG100400 Rh2BG101100 Rh2BG102200 Rh2CG103300 Rh2CG104600 Rh2DG100700 Rh2DG101500 Rh5AG211800 Rh5BG210700 Rh5CG234400 Rh5CG234500 Rh5DG216100
rosa_wichuraiana	Rw2G007570 Rw2G007630 Rw2G007640 Rw5G019420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1214
Acc65I	GGTACC	1 cut(s)	1965
AccB1I	GGYRCC	1 cut(s)	1965
AccI	GTMKAC	2 cut(s)	896, 1437
AciI	CCGC	2 cut(s)	665, 2214
AclWI	GGATC	8 cut(s)	46, 656, 690, 1263, 1322, 1620, 1772, 1832
AcoI	YGGCCR	5 cut(s)	179, 766, 1831, 1843, 1853
AcsI	RAATTY	6 cut(s)	433, 587, 928, 1557, 2050, 2195
AcuI	CTGAAG	1 cut(s)	1176
AdeI	CACNNNGTG	1 cut(s)	2108
AfaI	GTAC	7 cut(s)	785, 1298, 1575, 1637, 1745, 1912, 1967
AfiI	CCNNNNNNNGG	6 cut(s)	851, 1225, 1526, 1728, 1962, 2229
AflIII	ACRYGT	1 cut(s)	741
AjnI	CCWGG	2 cut(s)	412, 1474
AleI	CACNNNNGTG	1 cut(s)	175
AluBI	AGCT	8 cut(s)	63, 475, 715, 779, 981, 1669, 1763, 2246
AluI	AGCT	8 cut(s)	63, 475, 715, 779, 981, 1669, 1763, 2246
Alw26I	GTCTC	3 cut(s)	221, 1190, 1892
AlwI	GGATC	8 cut(s)	46, 656, 690, 1263, 1322, 1620, 1772, 1832
AlwNI	CAGNNNCTG	2 cut(s)	779, 953
Ama87I	CYCGRG	1 cut(s)	1193
AoxI	GGCC	7 cut(s)	179, 493, 766, 1190, 1831, 1843, 1853
ApeKI	GCWGC	4 cut(s)	400, 776, 779, 981
ApoI	RAATTY	6 cut(s)	433, 587, 928, 1557, 2050, 2195
ArsI	GACNNNNNNTTYG	2 cut(s)	378, 410
Asp718I	GGTACC	1 cut(s)	1965
AspS9I	GGNCC	4 cut(s)	353, 494, 1191, 1565
AsuHPI	GGTGA	3 cut(s)	1147, 1604, 2228
AsuII	TTCGAA	1 cut(s)	346
AvaI	CYCGRG	1 cut(s)	1193
AvaII	GGWCC	2 cut(s)	353, 1565
BaeGI	GKGCMC	1 cut(s)	2087
BalI	TGGCCA	2 cut(s)	181, 1833
BanI	GGYRCC	1 cut(s)	1965
BanII	GRGCYC	1 cut(s)	1426
BbsI	GAAGAC	1 cut(s)	229
BbvCI	CCTCAGC	1 cut(s)	678
BbvI	GCAGC	4 cut(s)	387, 766, 788, 968
BccI	CCATC	8 cut(s)	89, 110, 113, 879, 1956, 1988, 2047, 2070
BceAI	ACGGC	1 cut(s)	2207
BcgI	CGANNNNNNTGC	2 cut(s)	1157, 1191
BciT130I	CCWGG	2 cut(s)	414, 1476
BclI	TGATCA	2 cut(s)	1605, 2175
BcoDI	GTCTC	3 cut(s)	221, 1190, 1892
BfaI	CTAG	2 cut(s)	215, 1281
BisI	GCNGC	4 cut(s)	401, 777, 780, 982
BlsI	GCNGC	4 cut(s)	402, 778, 781, 983
Bme1390I	CCNGG	2 cut(s)	414, 1476
Bme18I	GGWCC	2 cut(s)	353, 1565
BmeT110I	CYCGRG	1 cut(s)	1193
BmgT120I	GGNCC	4 cut(s)	353, 494, 1191, 1565
BmiI	GGNNCC	2 cut(s)	1967, 2075
BmrFI	CCNGG	2 cut(s)	414, 1476
BmrI	ACTGGG	1 cut(s)	291
BmsI	GCATC	9 cut(s)	491, 544, 799, 1003, 1267, 1480, 1722, 1882, 2209
BmuI	ACTGGG	1 cut(s)	291
BoxI	GACNNNNGTC	1 cut(s)	233
BpiI	GAAGAC	1 cut(s)	229
BpmI	CTGGAG	1 cut(s)	1290
Bpu10I	CCTNAGC	2 cut(s)	668, 678
Bpu14I	TTCGAA	1 cut(s)	346
BpuEI	CTTGAG	2 cut(s)	471, 1301
Bsa29I	ATCGAT	1 cut(s)	114
BsaBI	GATNNNNATC	1 cut(s)	1604
BsaI	GGTCTC	1 cut(s)	1190
BsaJI	CCNNGG	4 cut(s)	201, 618, 888, 1906
BsaWI	WCCGGW	1 cut(s)	1728
BsaXI	ACNNNNNCTCC	2 cut(s)	1392, 1422
Bsc4I	CCNNNNNNNGG	6 cut(s)	851, 1225, 1526, 1728, 1962, 2229
Bse118I	RCCGGY	1 cut(s)	1845
Bse1I	ACTGG	8 cut(s)	257, 286, 582, 1375, 1558, 1568, 1581, 1781
Bse3DI	GCAATG	1 cut(s)	1000
Bse8I	GATNNNNATC	1 cut(s)	1604
BseBI	CCWGG	2 cut(s)	414, 1476
BseCI	ATCGAT	1 cut(s)	114
BseDI	CCNNGG	4 cut(s)	201, 618, 888, 1906
BseGI	GGATG	3 cut(s)	1471, 1737, 2062
BseJI	GATNNNNATC	1 cut(s)	1604
BseLI	CCNNNNNNNGG	6 cut(s)	851, 1225, 1526, 1728, 1962, 2229
BseMI	GCAATG	1 cut(s)	1000
BseMII	CTCAG	3 cut(s)	524, 669, 2178
BseNI	ACTGG	8 cut(s)	257, 286, 582, 1375, 1558, 1568, 1581, 1781
BseRI	GAGGAG	3 cut(s)	804, 1755, 2036
BseSI	GKGCMC	1 cut(s)	2087
BseXI	GCAGC	4 cut(s)	387, 766, 788, 968
BsgI	GTGCAG	1 cut(s)	386
BshFI	GGCC	7 cut(s)	181, 495, 768, 1192, 1833, 1845, 1855
BshNI	GGYRCC	1 cut(s)	1965
BshVI	ATCGAT	1 cut(s)	114
BsiHKCI	CYCGRG	1 cut(s)	1193
BsiSI	CCGG	2 cut(s)	1729, 1846
BsiWI	CGTACG	1 cut(s)	783
BslFI	GGGAC	2 cut(s)	1596, 2116
BslI	CCNNNNNNNGG	6 cut(s)	851, 1225, 1526, 1728, 1962, 2229
BsmAI	GTCTC	3 cut(s)	221, 1190, 1892
BsmFI	GGGAC	2 cut(s)	1596, 2116
BsmI	GAATGC	1 cut(s)	2156
BsnI	GGCC	7 cut(s)	181, 495, 768, 1192, 1833, 1845, 1855
Bso31I	GGTCTC	1 cut(s)	1190
BsoBI	CYCGRG	1 cut(s)	1193
Bsp119I	TTCGAA	1 cut(s)	346
Bsp1286I	GDGCHC	2 cut(s)	1426, 2087
Bsp19I	CCATGG	2 cut(s)	618, 888
BspACI	CCGC	2 cut(s)	665, 2214
BspANI	GGCC	7 cut(s)	181, 495, 768, 1192, 1833, 1845, 1855
BspCNI	CTCAG	3 cut(s)	523, 670, 2179
BspDI	ATCGAT	1 cut(s)	114
BspHI	TCATGA	1 cut(s)	91
BspLI	GGNNCC	2 cut(s)	1967, 2075
BspPI	GGATC	8 cut(s)	46, 656, 690, 1263, 1322, 1620, 1772, 1832
BspT104I	TTCGAA	1 cut(s)	346
BspT107I	GGYRCC	1 cut(s)	1965
BspTNI	GGTCTC	1 cut(s)	1190
BsrDI	GCAATG	1 cut(s)	1000
BsrFI	RCCGGY	1 cut(s)	1845
BsrI	ACTGG	8 cut(s)	257, 286, 582, 1375, 1558, 1568, 1581, 1781
BssAI	RCCGGY	1 cut(s)	1845
BssECI	CCNNGG	4 cut(s)	201, 618, 888, 1906
BssNAI	GTATAC	1 cut(s)	897
BssT1I	CCWWGG	3 cut(s)	201, 618, 888
Bst1107I	GTATAC	1 cut(s)	897
Bst2UI	CCWGG	2 cut(s)	414, 1476
Bst4CI	ACNGT	8 cut(s)	132, 316, 955, 1129, 1510, 1907, 2078, 2108
Bst6I	CTCTTC	3 cut(s)	1451, 1536, 2222
BstAPI	GCANNNNNTGC	2 cut(s)	409, 591
BstBI	TTCGAA	1 cut(s)	346
BstC8I	GCNNGC	2 cut(s)	2120, 2235
BstDEI	CTNAG	6 cut(s)	510, 668, 678, 1355, 1698, 2187
BstDSI	CCRYGG	3 cut(s)	618, 888, 1906
BstENI	CCTNNNNNAGG	1 cut(s)	1223
BstF5I	GGATG	3 cut(s)	1471, 1737, 2062
BstMAI	GTCTC	3 cut(s)	221, 1190, 1892
BstMWI	GCNNNNNNNGC	7 cut(s)	133, 409, 563, 591, 990, 2199, 2243
BstNI	CCWGG	2 cut(s)	414, 1476
BstNSI	RCATGY	2 cut(s)	745, 2122
BstPAI	GACNNNNGTC	1 cut(s)	233
BstSCI	CCNGG	2 cut(s)	412, 1474
BstSLI	GKGCMC	1 cut(s)	2087
BstV1I	GCAGC	4 cut(s)	387, 766, 788, 968
BstV2I	GAAGAC	1 cut(s)	229
BstX2I	RGATCY	3 cut(s)	661, 682, 1612
BstXI	CCANNNNNNTGG	2 cut(s)	177, 1841
BstYI	RGATCY	3 cut(s)	661, 682, 1612
BstZ17I	GTATAC	1 cut(s)	897
Bsu15I	ATCGAT	1 cut(s)	114
BsuRI	GGCC	7 cut(s)	181, 495, 768, 1192, 1833, 1845, 1855
BsuTUI	ATCGAT	1 cut(s)	114
BtgI	CCRYGG	3 cut(s)	618, 888, 1906
BtsCI	GGATG	3 cut(s)	1471, 1737, 2062
BtsI	GCAGTG	1 cut(s)	1101
BtsIMutI	CAGTG	5 cut(s)	951, 1008, 1101, 1368, 2113
Cac8I	GCNNGC	2 cut(s)	2120, 2235
CaiI	CAGNNNCTG	2 cut(s)	779, 953
CciI	TCATGA	1 cut(s)	91
Cfr10I	RCCGGY	1 cut(s)	1845
Cfr13I	GGNCC	4 cut(s)	353, 494, 1191, 1565
ClaI	ATCGAT	1 cut(s)	114
Csp6I	GTAC	7 cut(s)	784, 1297, 1574, 1636, 1744, 1911, 1966
CspCI	CAANNNNNGTGG	6 cut(s)	190, 225, 430, 465, 913, 948
CviQI	GTAC	7 cut(s)	784, 1297, 1574, 1636, 1744, 1911, 1966
DdeI	CTNAG	6 cut(s)	510, 668, 678, 1355, 1698, 2187
DraIII	CACNNNGTG	1 cut(s)	2108
DrdI	GACNNNNNNGTC	1 cut(s)	1214
DseDI	GACNNNNNNGTC	1 cut(s)	1214
EaeI	YGGCCR	5 cut(s)	179, 766, 1831, 1843, 1853
Eam1104I	CTCTTC	3 cut(s)	1451, 1536, 2222
EarI	CTCTTC	3 cut(s)	1451, 1536, 2222
Eco130I	CCWWGG	3 cut(s)	201, 618, 888
Eco24I	GRGCYC	1 cut(s)	1426
Eco31I	GGTCTC	1 cut(s)	1190
Eco47I	GGWCC	2 cut(s)	353, 1565
Eco57I	CTGAAG	1 cut(s)	1176
Eco88I	CYCGRG	1 cut(s)	1193
EcoNI	CCTNNNNNAGG	1 cut(s)	1223
EcoRII	CCWGG	2 cut(s)	412, 1474
EcoT14I	CCWWGG	3 cut(s)	201, 618, 888
EcoT22I	ATGCAT	2 cut(s)	1390, 2093
EcoT38I	GRGCYC	1 cut(s)	1426
ErhI	CCWWGG	3 cut(s)	201, 618, 888
FalI	AAGNNNNNCTT	8 cut(s)	459, 491, 651, 683, 1752, 1784, 1919, 1951
FaqI	GGGAC	2 cut(s)	1596, 2116
FauNDI	CATATG	4 cut(s)	358, 1390, 1870, 1915
FbaI	TGATCA	2 cut(s)	1605, 2175
FblI	GTMKAC	2 cut(s)	896, 1437
Fnu4HI	GCNGC	4 cut(s)	401, 777, 780, 982
FokI	GGATG	3 cut(s)	1458, 1744, 2049
FriOI	GRGCYC	1 cut(s)	1426
Fsp4HI	GCNGC	4 cut(s)	401, 777, 780, 982
FspBI	CTAG	2 cut(s)	215, 1281
GluI	GCNGC	4 cut(s)	401, 777, 780, 982
GsuI	CTGGAG	1 cut(s)	1290
HaeIII	GGCC	7 cut(s)	181, 495, 768, 1192, 1833, 1845, 1855
HapII	CCGG	2 cut(s)	1729, 1846
HincII	GTYRAC	2 cut(s)	1114, 1707
HindII	GTYRAC	2 cut(s)	1114, 1707
HindIII	AAGCTT	3 cut(s)	61, 473, 1667
HinfI	GANTC	7 cut(s)	286, 597, 971, 1169, 1215, 1351, 1682
HpaII	CCGG	2 cut(s)	1729, 1846
HphI	GGTGA	3 cut(s)	1147, 1604, 2228
Hpy166II	GTNNAC	6 cut(s)	212, 897, 1114, 1438, 1707, 1792
Hpy188I	TCNGA	5 cut(s)	513, 732, 876, 1521, 2188
Hpy8I	GTNNAC	6 cut(s)	212, 897, 1114, 1438, 1707, 1792
Hpy99I	CGWCG	2 cut(s)	267, 824
HpyAV	CCTTC	5 cut(s)	320, 1085, 1132, 1945, 2118
HpyCH4III	ACNGT	8 cut(s)	132, 316, 955, 1129, 1510, 1907, 2078, 2108
HpyCH4IV	ACGT	3 cut(s)	786, 1434, 1971
HpyF10VI	GCNNNNNNNGC	7 cut(s)	133, 409, 563, 591, 990, 2199, 2243
HpyF3I	CTNAG	6 cut(s)	510, 668, 678, 1355, 1698, 2187
HpySE526I	ACGT	3 cut(s)	786, 1434, 1971
KpnI	GGTACC	1 cut(s)	1969
Ksp22I	TGATCA	2 cut(s)	1605, 2175
LmnI	GCTCC	2 cut(s)	817, 1768
Lsp1109I	GCAGC	4 cut(s)	387, 766, 788, 968
LweI	GCATC	9 cut(s)	491, 544, 799, 1003, 1267, 1480, 1722, 1882, 2209
MaeI	CTAG	2 cut(s)	215, 1281
MaeII	ACGT	3 cut(s)	786, 1434, 1971
MaeIII	GTNAC	5 cut(s)	787, 916, 1688, 1814, 1898
MfeI	CAATTG	3 cut(s)	222, 1939, 2007
MflI	RGATCY	3 cut(s)	661, 682, 1612
MhlI	GDGCHC	2 cut(s)	1426, 2087
MlsI	TGGCCA	2 cut(s)	181, 1833
MluNI	TGGCCA	2 cut(s)	181, 1833
MlyI	GAGTC	3 cut(s)	980, 1224, 1345
MmeI	TCCRAC	4 cut(s)	36, 1492, 1624, 2051
Mox20I	TGGCCA	2 cut(s)	181, 1833
Mph1103I	ATGCAT	2 cut(s)	1390, 2093
MscI	TGGCCA	2 cut(s)	181, 1833
MseI	TTAA	6 cut(s)	59, 192, 642, 1131, 1395, 1725
MslI	CAYNNNNRTG	4 cut(s)	175, 912, 1389, 1627
Msp20I	TGGCCA	2 cut(s)	181, 1833
MspA1I	CMGCKG	1 cut(s)	779
MspI	CCGG	2 cut(s)	1729, 1846
MspR9I	CCNGG	2 cut(s)	414, 1476
MunI	CAATTG	3 cut(s)	222, 1939, 2007
Mva1269I	GAATGC	1 cut(s)	2156
MvaI	CCWGG	2 cut(s)	414, 1476
MwoI	GCNNNNNNNGC	7 cut(s)	133, 409, 563, 591, 990, 2199, 2243
NcoI	CCATGG	2 cut(s)	618, 888
NdeI	CATATG	4 cut(s)	358, 1390, 1870, 1915
NlaIV	GGNNCC	2 cut(s)	1967, 2075
NmeAIII	GCCGAG	1 cut(s)	1881
NmuCI	GTSAC	3 cut(s)	787, 1814, 1898
NsiI	ATGCAT	2 cut(s)	1390, 2093
NspI	RCATGY	2 cut(s)	745, 2122
NspV	TTCGAA	1 cut(s)	346
OliI	CACNNNNGTG	1 cut(s)	175
PaeI	GCATGC	1 cut(s)	2122
PagI	TCATGA	1 cut(s)	91
PciI	ACATGT	1 cut(s)	741
PctI	GAATGC	1 cut(s)	2156
PfeI	GAWTC	4 cut(s)	286, 597, 1169, 1682
Pfl23II	CGTACG	1 cut(s)	783
PfoI	TCCNGGA	1 cut(s)	1474
PkrI	GCNGC	4 cut(s)	402, 778, 781, 983
PleI	GAGTC	3 cut(s)	979, 1223, 1345
PpsI	GAGTC	3 cut(s)	979, 1223, 1345
PscI	ACATGT	1 cut(s)	741
PshAI	GACNNNNGTC	1 cut(s)	233
Psp6I	CCWGG	2 cut(s)	412, 1474
PspGI	CCWGG	2 cut(s)	412, 1474
PspLI	CGTACG	1 cut(s)	783
PspN4I	GGNNCC	2 cut(s)	1967, 2075
PspPI	GGNCC	4 cut(s)	353, 494, 1191, 1565
PstNI	CAGNNNCTG	2 cut(s)	779, 953
PsuI	RGATCY	3 cut(s)	661, 682, 1612
PvuII	CAGCTG	1 cut(s)	779
RsaI	GTAC	7 cut(s)	785, 1298, 1575, 1637, 1745, 1912, 1967
RsaNI	GTAC	7 cut(s)	784, 1297, 1574, 1636, 1744, 1911, 1966
RseI	CAYNNNNRTG	4 cut(s)	175, 912, 1389, 1627
SaqAI	TTAA	6 cut(s)	59, 192, 642, 1131, 1395, 1725
SatI	GCNGC	4 cut(s)	401, 777, 780, 982
Sau96I	GGNCC	4 cut(s)	353, 494, 1191, 1565
SchI	GAGTC	3 cut(s)	980, 1224, 1345
ScrFI	CCNGG	2 cut(s)	414, 1476
SduI	GDGCHC	2 cut(s)	1426, 2087
SfaNI	GCATC	9 cut(s)	491, 544, 799, 1003, 1267, 1480, 1722, 1882, 2209
SfuI	TTCGAA	1 cut(s)	346
SinI	GGWCC	2 cut(s)	353, 1565
SmiMI	CAYNNNNRTG	4 cut(s)	175, 912, 1389, 1627
SmlI	CTYRAG	2 cut(s)	486, 1316
SmoI	CTYRAG	2 cut(s)	486, 1316
SphI	GCATGC	1 cut(s)	2122
SsiI	CCGC	2 cut(s)	665, 2214
SspMI	CTAG	2 cut(s)	215, 1281
StyD4I	CCNGG	2 cut(s)	412, 1474
StyI	CCWWGG	3 cut(s)	201, 618, 888
TaaI	ACNGT	8 cut(s)	132, 316, 955, 1129, 1510, 1907, 2078, 2108
TaiI	ACGT	3 cut(s)	789, 1437, 1974
TaqI	TCGA	7 cut(s)	114, 160, 346, 604, 819, 1890, 1978
TatI	WGTACW	1 cut(s)	1573
TfiI	GAWTC	4 cut(s)	286, 597, 1169, 1682
Tru1I	TTAA	6 cut(s)	59, 192, 642, 1131, 1395, 1725
Tru9I	TTAA	6 cut(s)	59, 192, 642, 1131, 1395, 1725
TscAI	CASTG	5 cut(s)	958, 1015, 1108, 1375, 2113
TseFI	GTSAC	3 cut(s)	787, 1814, 1898
TseI	GCWGC	4 cut(s)	400, 776, 779, 981
Tsp45I	GTSAC	3 cut(s)	787, 1814, 1898
TspGWI	ACGGA	3 cut(s)	543, 1542, 1724
TspRI	CASTG	5 cut(s)	958, 1015, 1108, 1375, 2113
VpaK11BI	GGWCC	2 cut(s)	353, 1565
XagI	CCTNNNNNAGG	1 cut(s)	1223
XapI	RAATTY	6 cut(s)	433, 587, 928, 1557, 2050, 2195
XceI	RCATGY	2 cut(s)	745, 2122
XmiI	GTMKAC	2 cut(s)	896, 1437
XspI	CTAG	2 cut(s)	215, 1281
Zsp2I	ATGCAT	2 cut(s)	1390, 2093

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.