Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001959

Physical Location & Seq

Genomic Coordinates Forward (+)

17882067 .. 17886090

Gene Structure 4024 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1221000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2484 bp

ATGGCTTTTTCCAACTCTTTTAGTTTTGCGTTTTGTTTCTGTTTTTCTGTGATCCTTTTAAGCTTTTATGCTAATGCTACTGATGTTTCTCATGATGGGAGAGCCATCACAATCGATGGTAAAAGGCGACTGTTGCTTTCGGGTTCAATCCACTATCCTCGAAGCACACCACAAATGTGGCCAGACTTGATTAAGAAGTCCAAGGAGGGTGGACTAGACACAATTGAGACTTATGTCTTCTGGAATGTGCATGAGCCAGTTCGTCGTCAGTATGATTTTACTGGGAATCTTGACCTTGTTAGATTTCTCAAAACAGTTCAAGATGAAGGACTTTATGCTGTTCTTCGAATTGGTCCATATGTTTGTGCCGAATGGAATTATGGAGGACTTCCCGTTTGGCTGCACAACTTGCCTGGCTGTGAGATCCGAACCGCAAATGATGTCTACATGAATGAGATGAAGAATTTTACCACATTGATTGTTGATATGATGAAAAAAGAAAAGCTTTTTGCATCTCAAGGTGGCCCAATTATTATTGCTCAGATTGAAAATGAATACGGAAATGTGCAATCATACTATGGAGATGCTGGTAAAGCATATATGAACTGGTGTGCAAATTTTGCCGAATCTCTCGACATTGGAGTTCCATGGATTATGTGCCAACAAAGTGATGCTCCTCAACCAATGATGAACACATGCAATGGTTGGTACTGTGATTCATTTACTCCAAATAATGAGAACAGTCCCAAGATGTGGACCGAAAATTGGACTGGATGGTTTAAGAGTTGGGGAGGCAAAGATCCGCTTAGGACTGCTGAGGATCTTGCTTTCTCTGTTGCAAGATTTTTCCAGCTTGGAGGCACATTTCAGAACTACTACATGTATCATGGTGGAACAAACTTTGGCCGAACAGCAGCTGCGTACGTCACTACTACTTATGATTACGATGCTCCTCTCGACGAATATGGCAACTTGAACCAACCAAAATGGGGGCATTTGAAAGAACTCCATCAGATTTTGAAATCCATGGAGTATACTCTCACACATGGGAATGTTACAACCACAAATTTTGACAATTCTGTTTCAGCCACTGTGTATGCAACAAATGAGTCATCAAGCTGCTTTTTGGGCAATGCAAATAGCACCACTGATGCTACAATCACTTTTCAAGGGAATACATACACCATTCCTGCTTGGTCTGTTAGTATTCTTCCTGATTGCAAAACAGAAGGATATAACACTGCAAAGGTCAACACACAAACAACAGTTAAGGTGAAGGTAGGGAACAAGGCTGAAGATGAACCCGAATCACTTGCTTGGTCGTGGAGGCCCGAGACCATTGATGACACCAGAGTCCTTGCAAAGGGAGAAATTACTGCAAATCAACTTTTGGATCAAAAGGTTGCTGGAGATGATGCTAGTGATTATCTTTGGTACATGACAAGTGTTCATCTCAAGAAAGATGATCCAATTTGGAGTAAGAACATGACTCTAAGGGTCAATGGCACTGGTTTCATTCTTCATGCATATGTTAATGGAGAATATGTTGGTTCTCAATGGGCTCAATACAACGTCTACAACTATGTATTTGAGAAGAGTGTCAAGTTGCATCCTGGAAAGAACATTATTTCATTGTTGAGTGCTACCGTTGGATTTCCGAATTACGGAGGTGGATTTGAAGAGATCAAAACTGGAATTTCTGGTCCAGTTGTACTGGTGGGACAAAATGGTGATGAGATTGTGATCAAGGATCTATCAGCACACAAATGGTCGTACAAGGTTGGACTTCATGGTTTGAAAAACAAGCTTTTTAGCACCGATTCTCGTTACGCTACTAAGTGGTCAACGGAAAACTTACCCATTAACCGGATGCTGACATGGTACAAGGCAACTTTCAAAGCTCCTCTTGGAACTGATCCAGTTGTGGTGGACTTACAAGGTTTGGGAAAGGGTCACGCTTGGATCAATGGCCATAGTATTGGCCGGTATTGGCCGAGTTATTTGTCATATGATACTGATTGTAGTCTCGATGCTTGTGACTACCGTGGTACATATGACAACAACAAGTGTTCCTTCAATTGTGGCAATCCTACCCAAAGATGGTACCACGTTCCTCGATCATTTATGGAAGATGGAGAAAATGCAATTGTGTTGTTTGAGGAGTTTGGTGGCAATCCATCTCTTGTGAATTTCCAAACCATCCAAGTTGGAACCGTCTGTGCCCATGCATACGAGAAGCACACAGTGGAATTGGCATGCGAAGGTCGTCCCATTTCTGCAATCAAGTTCGCTAGCTTTGGTAATTCCCAAGGAACCTGTGGATCATTTGAGAAAGGCAGCTGTGATTCTGGACGTGCATTGTCGATCCTTAAAAAGGAATGCGTGGGCAAGGAGAAGTGCTTGATCAATGTTTCTGAGAGCAAATTTGGCTTGACAAACTGCGGTGATGCCGTGAAGAGGCTGGCTGTGGAAGCTGTTTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BetaGal_dom4_5 Gal_Lectin Glyco_hydro_35

Protein Analysis

828

Amino Acids

92.5

Weight (kDa)

5.82

Isoelectric Point (pI)

31.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	36 - 338	1.3e-119	Glycosyl hydrolases family 35
Glyco_hydro_42	PF02449	57 - 206	4.9e-07	Beta-galactosidase
GHD	PF17834	363 - 417	5.2e-23	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	625 - 714	9.6e-25	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	752 - 827	1.1e-15	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001959)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31740 AT2G04060 AT5G20710 AT5G20710
fragaria_vesca	FvH4_1g09120 FvH4_1g09190 FvH4_1g09190 FvH4_1g26380
malus_domestica	MD02G1096400.v1.1 MD03G1276000.v1.1 MD15G1220900.v1.1 MD15G1221000.v1.1
prunus_persica	Prupe.7G194500_v2.0.a1
pyrus_communis	pycom02g07640
rosa_chinensis	RchiOBHm_Chr2g0095511 RchiOBHm_Chr2g0095591 RchiOBHm_Chr5g0030701
rosa_laevigata	RLG00000016574 RLG00000016582 RLG00000016583
rosa_multiflora	Rmu_co8462209.1_g000001 Rmu_sc0003238.1_g000025 Rmu_sc0003238.1_g000028 Rmu_sc0004719.1_g000009
rosa_roxburghii	Rroxscaffold_2G00146240
rosa_rugosa	Rorug02G0051800 Rorug02G0052600 Rorug05G0120200
rosa_samantha	Rh2AG099800 Rh2AG100400 Rh2BG101100 Rh2BG102200 Rh2CG103300 Rh2CG104600 Rh2DG100700 Rh2DG101500 Rh5AG211800 Rh5BG210700 Rh5CG234400 Rh5CG234500 Rh5DG216100
rosa_wichuraiana	Rw2G007570 Rw2G007630 Rw2G007640 Rw5G019420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1352
Acc65I	GGTACC	1 cut(s)	2103
AccB1I	GGYRCC	1 cut(s)	2103
AccB7I	CCANNNNNTGG	1 cut(s)	753
AccI	GTMKAC	3 cut(s)	444, 1034, 1575
AciI	CCGC	3 cut(s)	432, 803, 2442
AcoI	YGGCCR	5 cut(s)	179, 904, 1969, 1981, 1991
AcsI	RAATTY	6 cut(s)	463, 616, 1066, 1695, 2188, 2423
AcuI	CTGAAG	1 cut(s)	1314
AdeI	CACNNNGTG	1 cut(s)	2245
AfaI	GTAC	8 cut(s)	710, 923, 1436, 1713, 1775, 1883, 2050, 2105
AfiI	CCNNNNNNNGG	9 cut(s)	753, 765, 989, 1363, 1664, 1866, 2100, 2374, 2457
AflIII	ACRYGT	1 cut(s)	879
AjiI	CACGTC	1 cut(s)	2354
AjnI	CCWGG	2 cut(s)	412, 1612
AleI	CACNNNNGTG	1 cut(s)	175
Alw26I	GTCTC	3 cut(s)	221, 1328, 2030
AlwNI	CAGNNNCTG	2 cut(s)	917, 1091
Ama87I	CYCGRG	1 cut(s)	1331
AoxI	GGCC	7 cut(s)	179, 523, 904, 1328, 1969, 1981, 1991
ApeKI	GCWGC	5 cut(s)	400, 914, 917, 1119, 2337
ApoI	RAATTY	6 cut(s)	463, 616, 1066, 1695, 2188, 2423
ArsI	GACNNNNNNTTYG	2 cut(s)	378, 410
Asp718I	GGTACC	1 cut(s)	2103
AspS9I	GGNCC	5 cut(s)	353, 524, 756, 1329, 1703
AsuHPI	GGTGA	3 cut(s)	1285, 1742, 2456
AsuII	TTCGAA	1 cut(s)	346
AsuNHI	GCTAGC	1 cut(s)	2291
AvaI	CYCGRG	1 cut(s)	1331
AvaII	GGWCC	3 cut(s)	353, 756, 1703
BaeGI	GKGCMC	1 cut(s)	2224
BalI	TGGCCA	2 cut(s)	181, 1971
BanI	GGYRCC	1 cut(s)	2103
BanII	GRGCYC	1 cut(s)	1564
BbsI	GAAGAC	1 cut(s)	229
BbvCI	CCTCAGC	1 cut(s)	816
BbvI	GCAGC	5 cut(s)	387, 904, 926, 1106, 2349
BccI	CCATC	9 cut(s)	89, 110, 113, 768, 1017, 2094, 2126, 2185, 2207
BceAI	ACGGC	1 cut(s)	2435
BcgI	CGANNNNNNTGC	2 cut(s)	1295, 1329
BciT130I	CCWGG	2 cut(s)	414, 1614
BclI	TGATCA	2 cut(s)	1743, 2403
BcoDI	GTCTC	3 cut(s)	221, 1328, 2030
BfaI	CTAG	3 cut(s)	215, 1419, 2292
BisI	GCNGC	5 cut(s)	401, 915, 918, 1120, 2338
BlsI	GCNGC	5 cut(s)	402, 916, 919, 1121, 2339
Bme1390I	CCNGG	2 cut(s)	414, 1614
Bme18I	GGWCC	3 cut(s)	353, 756, 1703
BmeT110I	CYCGRG	1 cut(s)	1331
BmgBI	CACGTC	1 cut(s)	2354
BmgT120I	GGNCC	5 cut(s)	353, 524, 756, 1329, 1703
BmiI	GGNNCC	3 cut(s)	2105, 2212, 2314
BmrFI	CCNGG	2 cut(s)	414, 1614
BmrI	ACTGGG	1 cut(s)	291
BmtI	GCTAGC	1 cut(s)	2295
BmuI	ACTGGG	1 cut(s)	291
BoxI	GACNNNNGTC	1 cut(s)	233
BpiI	GAAGAC	1 cut(s)	229
BpmI	CTGGAG	1 cut(s)	1428
Bpu10I	CCTNAGC	2 cut(s)	806, 816
Bpu14I	TTCGAA	1 cut(s)	346
BpuEI	CTTGAG	2 cut(s)	501, 1439
Bsa29I	ATCGAT	1 cut(s)	114
BsaBI	GATNNNNATC	1 cut(s)	1742
BsaI	GGTCTC	1 cut(s)	1328
BsaJI	CCNNGG	5 cut(s)	201, 647, 1026, 2044, 2308
BsaWI	WCCGGW	1 cut(s)	1866
BsaXI	ACNNNNNCTCC	2 cut(s)	1530, 1560
Bsc4I	CCNNNNNNNGG	9 cut(s)	753, 765, 989, 1363, 1664, 1866, 2100, 2374, 2457
Bse118I	RCCGGY	1 cut(s)	1983
Bse1I	ACTGG	9 cut(s)	257, 286, 611, 775, 1513, 1696, 1706, 1719, 1919
Bse3DI	GCAATG	2 cut(s)	706, 1138
Bse8I	GATNNNNATC	1 cut(s)	1742
BseBI	CCWGG	2 cut(s)	414, 1614
BseCI	ATCGAT	1 cut(s)	114
BseDI	CCNNGG	5 cut(s)	201, 647, 1026, 2044, 2308
BseGI	GGATG	4 cut(s)	779, 1609, 1875, 2199
BseJI	GATNNNNATC	1 cut(s)	1742
BseLI	CCNNNNNNNGG	9 cut(s)	753, 765, 989, 1363, 1664, 1866, 2100, 2374, 2457
BseMI	GCAATG	2 cut(s)	706, 1138
BseMII	CTCAG	3 cut(s)	554, 807, 2406
BseNI	ACTGG	9 cut(s)	257, 286, 611, 775, 1513, 1696, 1706, 1719, 1919
BseRI	GAGGAG	4 cut(s)	666, 942, 1893, 2174
BseSI	GKGCMC	1 cut(s)	2224
BseXI	GCAGC	5 cut(s)	387, 904, 926, 1106, 2349
BsgI	GTGCAG	1 cut(s)	386
BshFI	GGCC	7 cut(s)	181, 525, 906, 1330, 1971, 1983, 1993
BshNI	GGYRCC	1 cut(s)	2103
BshVI	ATCGAT	1 cut(s)	114
BsiHKCI	CYCGRG	1 cut(s)	1331
BsiSI	CCGG	2 cut(s)	1867, 1984
BsiWI	CGTACG	1 cut(s)	921
BslFI	GGGAC	3 cut(s)	729, 1734, 2253
BslI	CCNNNNNNNGG	9 cut(s)	753, 765, 989, 1363, 1664, 1866, 2100, 2374, 2457
BsmAI	GTCTC	3 cut(s)	221, 1328, 2030
BsmFI	GGGAC	3 cut(s)	729, 1734, 2253
BsmI	GAATGC	1 cut(s)	2384
BsnI	GGCC	7 cut(s)	181, 525, 906, 1330, 1971, 1983, 1993
Bso31I	GGTCTC	1 cut(s)	1328
BsoBI	CYCGRG	1 cut(s)	1331
Bsp119I	TTCGAA	1 cut(s)	346
Bsp1286I	GDGCHC	2 cut(s)	1564, 2224
Bsp19I	CCATGG	2 cut(s)	647, 1026
BspACI	CCGC	3 cut(s)	432, 803, 2442
BspANI	GGCC	7 cut(s)	181, 525, 906, 1330, 1971, 1983, 1993
BspCNI	CTCAG	3 cut(s)	553, 808, 2407
BspDI	ATCGAT	1 cut(s)	114
BspHI	TCATGA	1 cut(s)	91
BspLI	GGNNCC	3 cut(s)	2105, 2212, 2314
BspOI	GCTAGC	1 cut(s)	2295
BspT104I	TTCGAA	1 cut(s)	346
BspT107I	GGYRCC	1 cut(s)	2103
BspTNI	GGTCTC	1 cut(s)	1328
BsrDI	GCAATG	2 cut(s)	706, 1138
BsrFI	RCCGGY	1 cut(s)	1983
BsrI	ACTGG	9 cut(s)	257, 286, 611, 775, 1513, 1696, 1706, 1719, 1919
BssAI	RCCGGY	1 cut(s)	1983
BssECI	CCNNGG	5 cut(s)	201, 647, 1026, 2044, 2308
BssNAI	GTATAC	1 cut(s)	1035
BssT1I	CCWWGG	4 cut(s)	201, 647, 1026, 2308
Bst1107I	GTATAC	1 cut(s)	1035
Bst2UI	CCWGG	2 cut(s)	414, 1614
Bst6I	CTCTTC	3 cut(s)	1589, 1674, 2450
BstAPI	GCANNNNNTGC	2 cut(s)	409, 620
BstBI	TTCGAA	1 cut(s)	346
BstC8I	GCNNGC	3 cut(s)	2257, 2293, 2463
BstDEI	CTNAG	6 cut(s)	540, 806, 816, 1493, 1836, 2415
BstDSI	CCRYGG	3 cut(s)	647, 1026, 2044
BstENI	CCTNNNNNAGG	2 cut(s)	1361, 2372
BstF5I	GGATG	4 cut(s)	779, 1609, 1875, 2199
BstMAI	GTCTC	3 cut(s)	221, 1328, 2030
BstMWI	GCNNNNNNNGC	7 cut(s)	133, 409, 593, 620, 1128, 2427, 2471
BstNI	CCWGG	2 cut(s)	414, 1614
BstNSI	RCATGY	3 cut(s)	699, 883, 2259
BstPAI	GACNNNNGTC	1 cut(s)	233
BstSCI	CCNGG	2 cut(s)	412, 1612
BstSLI	GKGCMC	1 cut(s)	2224
BstV1I	GCAGC	5 cut(s)	387, 904, 926, 1106, 2349
BstV2I	GAAGAC	1 cut(s)	229
BstX2I	RGATCY	4 cut(s)	423, 799, 820, 1750
BstXI	CCANNNNNNTGG	2 cut(s)	177, 1979
BstYI	RGATCY	4 cut(s)	423, 799, 820, 1750
BstZ17I	GTATAC	1 cut(s)	1035
Bsu15I	ATCGAT	1 cut(s)	114
BsuRI	GGCC	7 cut(s)	181, 525, 906, 1330, 1971, 1983, 1993
BsuTUI	ATCGAT	1 cut(s)	114
BtgI	CCRYGG	3 cut(s)	647, 1026, 2044
BtrI	CACGTC	1 cut(s)	2354
BtsCI	GGATG	4 cut(s)	779, 1609, 1875, 2199
BtsI	GCAGTG	1 cut(s)	1239
BtsIMutI	CAGTG	5 cut(s)	1089, 1146, 1239, 1506, 2250
Cac8I	GCNNGC	3 cut(s)	2257, 2293, 2463
CaiI	CAGNNNCTG	2 cut(s)	917, 1091
CciI	TCATGA	1 cut(s)	91
Cfr10I	RCCGGY	1 cut(s)	1983
Cfr13I	GGNCC	5 cut(s)	353, 524, 756, 1329, 1703
ClaI	ATCGAT	1 cut(s)	114
Csp6I	GTAC	8 cut(s)	709, 922, 1435, 1712, 1774, 1882, 2049, 2104
CspCI	CAANNNNNGTGG	6 cut(s)	190, 225, 460, 495, 1051, 1086
CviQI	GTAC	8 cut(s)	709, 922, 1435, 1712, 1774, 1882, 2049, 2104
DdeI	CTNAG	6 cut(s)	540, 806, 816, 1493, 1836, 2415
DraIII	CACNNNGTG	1 cut(s)	2245
DrdI	GACNNNNNNGTC	1 cut(s)	1352
DseDI	GACNNNNNNGTC	1 cut(s)	1352
EaeI	YGGCCR	5 cut(s)	179, 904, 1969, 1981, 1991
Eam1104I	CTCTTC	3 cut(s)	1589, 1674, 2450
EarI	CTCTTC	3 cut(s)	1589, 1674, 2450
Eco130I	CCWWGG	4 cut(s)	201, 647, 1026, 2308
Eco24I	GRGCYC	1 cut(s)	1564
Eco31I	GGTCTC	1 cut(s)	1328
Eco47I	GGWCC	3 cut(s)	353, 756, 1703
Eco57I	CTGAAG	1 cut(s)	1314
Eco88I	CYCGRG	1 cut(s)	1331
EcoNI	CCTNNNNNAGG	2 cut(s)	1361, 2372
EcoRII	CCWGG	2 cut(s)	412, 1612
EcoT14I	CCWWGG	4 cut(s)	201, 647, 1026, 2308
EcoT22I	ATGCAT	2 cut(s)	1528, 2230
EcoT38I	GRGCYC	1 cut(s)	1564
ErhI	CCWWGG	4 cut(s)	201, 647, 1026, 2308
FalI	AAGNNNNNCTT	8 cut(s)	489, 521, 789, 821, 1890, 1922, 2057, 2089
FaqI	GGGAC	3 cut(s)	729, 1734, 2253
FauNDI	CATATG	4 cut(s)	358, 1528, 2008, 2053
FbaI	TGATCA	2 cut(s)	1743, 2403
FblI	GTMKAC	3 cut(s)	444, 1034, 1575
Fnu4HI	GCNGC	5 cut(s)	401, 915, 918, 1120, 2338
FokI	GGATG	4 cut(s)	786, 1596, 1882, 2186
FriOI	GRGCYC	1 cut(s)	1564
Fsp4HI	GCNGC	5 cut(s)	401, 915, 918, 1120, 2338
FspBI	CTAG	3 cut(s)	215, 1419, 2292
GluI	GCNGC	5 cut(s)	401, 915, 918, 1120, 2338
GsuI	CTGGAG	1 cut(s)	1428
HaeIII	GGCC	7 cut(s)	181, 525, 906, 1330, 1971, 1983, 1993
HapII	CCGG	2 cut(s)	1867, 1984
HincII	GTYRAC	2 cut(s)	1252, 1845
HindII	GTYRAC	2 cut(s)	1252, 1845
HindIII	AAGCTT	3 cut(s)	61, 503, 1805
HinfI	GANTC	9 cut(s)	286, 626, 716, 1109, 1307, 1353, 1489, 1820, 2345
HpaII	CCGG	2 cut(s)	1867, 1984
HphI	GGTGA	3 cut(s)	1285, 1742, 2456
Hpy166II	GTNNAC	8 cut(s)	212, 445, 756, 1035, 1252, 1576, 1845, 1930
Hpy188I	TCNGA	6 cut(s)	428, 543, 870, 1014, 1659, 2416
Hpy8I	GTNNAC	8 cut(s)	212, 445, 756, 1035, 1252, 1576, 1845, 1930
Hpy99I	CGWCG	2 cut(s)	267, 962
HpyAV	CCTTC	5 cut(s)	320, 1223, 1270, 2083, 2255
HpyCH4IV	ACGT	4 cut(s)	924, 1572, 2109, 2353
HpyF10VI	GCNNNNNNNGC	7 cut(s)	133, 409, 593, 620, 1128, 2427, 2471
HpyF3I	CTNAG	6 cut(s)	540, 806, 816, 1493, 1836, 2415
HpySE526I	ACGT	4 cut(s)	924, 1572, 2109, 2353
KpnI	GGTACC	1 cut(s)	2107
Ksp22I	TGATCA	2 cut(s)	1743, 2403
LmnI	GCTCC	3 cut(s)	679, 955, 1906
Lsp1109I	GCAGC	5 cut(s)	387, 904, 926, 1106, 2349
MaeI	CTAG	3 cut(s)	215, 1419, 2292
MaeII	ACGT	4 cut(s)	924, 1572, 2109, 2353
MaeIII	GTNAC	5 cut(s)	925, 1054, 1826, 1952, 2036
MfeI	CAATTG	3 cut(s)	222, 2077, 2145
MflI	RGATCY	4 cut(s)	423, 799, 820, 1750
MhlI	GDGCHC	2 cut(s)	1564, 2224
MlsI	TGGCCA	2 cut(s)	181, 1971
MluNI	TGGCCA	2 cut(s)	181, 1971
MlyI	GAGTC	3 cut(s)	1118, 1362, 1483
MmeI	TCCRAC	4 cut(s)	36, 1630, 1762, 2188
Mox20I	TGGCCA	2 cut(s)	181, 1971
Mph1103I	ATGCAT	2 cut(s)	1528, 2230
MscI	TGGCCA	2 cut(s)	181, 1971
MseI	TTAA	7 cut(s)	59, 192, 780, 1269, 1533, 1863, 2370
MslI	CAYNNNNRTG	4 cut(s)	175, 1050, 1527, 1765
Msp20I	TGGCCA	2 cut(s)	181, 1971
MspA1I	CMGCKG	2 cut(s)	917, 2340
MspI	CCGG	2 cut(s)	1867, 1984
MspR9I	CCNGG	2 cut(s)	414, 1614
MunI	CAATTG	3 cut(s)	222, 2077, 2145
Mva1269I	GAATGC	1 cut(s)	2384
MvaI	CCWGG	2 cut(s)	414, 1614
MwoI	GCNNNNNNNGC	7 cut(s)	133, 409, 593, 620, 1128, 2427, 2471
NcoI	CCATGG	2 cut(s)	647, 1026
NdeI	CATATG	4 cut(s)	358, 1528, 2008, 2053
NheI	GCTAGC	1 cut(s)	2291
NlaIV	GGNNCC	3 cut(s)	2105, 2212, 2314
NmeAIII	GCCGAG	1 cut(s)	2019
NmuCI	GTSAC	3 cut(s)	925, 1952, 2036
NsiI	ATGCAT	2 cut(s)	1528, 2230
NspI	RCATGY	3 cut(s)	699, 883, 2259
NspV	TTCGAA	1 cut(s)	346
OliI	CACNNNNGTG	1 cut(s)	175
PaeI	GCATGC	1 cut(s)	2259
PagI	TCATGA	1 cut(s)	91
PciI	ACATGT	1 cut(s)	879
PctI	GAATGC	1 cut(s)	2384
PfeI	GAWTC	6 cut(s)	286, 626, 716, 1307, 1820, 2345
Pfl23II	CGTACG	1 cut(s)	921
PflMI	CCANNNNNTGG	1 cut(s)	753
PfoI	TCCNGGA	1 cut(s)	1612
PkrI	GCNGC	5 cut(s)	402, 916, 919, 1121, 2339
PleI	GAGTC	3 cut(s)	1117, 1361, 1483
PpsI	GAGTC	3 cut(s)	1117, 1361, 1483
PscI	ACATGT	1 cut(s)	879
PshAI	GACNNNNGTC	1 cut(s)	233
Psp6I	CCWGG	2 cut(s)	412, 1612
PspGI	CCWGG	2 cut(s)	412, 1612
PspLI	CGTACG	1 cut(s)	921
PspN4I	GGNNCC	3 cut(s)	2105, 2212, 2314
PspPI	GGNCC	5 cut(s)	353, 524, 756, 1329, 1703
PstNI	CAGNNNCTG	2 cut(s)	917, 1091
PsuI	RGATCY	4 cut(s)	423, 799, 820, 1750
PvuII	CAGCTG	2 cut(s)	917, 2340
RsaI	GTAC	8 cut(s)	710, 923, 1436, 1713, 1775, 1883, 2050, 2105
RsaNI	GTAC	8 cut(s)	709, 922, 1435, 1712, 1774, 1882, 2049, 2104
RseI	CAYNNNNRTG	4 cut(s)	175, 1050, 1527, 1765
SaqAI	TTAA	7 cut(s)	59, 192, 780, 1269, 1533, 1863, 2370
SatI	GCNGC	5 cut(s)	401, 915, 918, 1120, 2338
Sau96I	GGNCC	5 cut(s)	353, 524, 756, 1329, 1703
SchI	GAGTC	3 cut(s)	1118, 1362, 1483
ScrFI	CCNGG	2 cut(s)	414, 1614
SduI	GDGCHC	2 cut(s)	1564, 2224
SfuI	TTCGAA	1 cut(s)	346
SinI	GGWCC	3 cut(s)	353, 756, 1703
SmiMI	CAYNNNNRTG	4 cut(s)	175, 1050, 1527, 1765
SmlI	CTYRAG	2 cut(s)	516, 1454
SmoI	CTYRAG	2 cut(s)	516, 1454
SphI	GCATGC	1 cut(s)	2259
SsiI	CCGC	3 cut(s)	432, 803, 2442
SspMI	CTAG	3 cut(s)	215, 1419, 2292
StyD4I	CCNGG	2 cut(s)	412, 1612
StyI	CCWWGG	4 cut(s)	201, 647, 1026, 2308
TaiI	ACGT	4 cut(s)	927, 1575, 2112, 2356
TaqI	TCGA	8 cut(s)	114, 160, 346, 633, 957, 2028, 2116, 2363
TaqII	GACCGA	1 cut(s)	773
TatI	WGTACW	1 cut(s)	1711
TfiI	GAWTC	6 cut(s)	286, 626, 716, 1307, 1820, 2345
Tru1I	TTAA	7 cut(s)	59, 192, 780, 1269, 1533, 1863, 2370
Tru9I	TTAA	7 cut(s)	59, 192, 780, 1269, 1533, 1863, 2370
TscAI	CASTG	5 cut(s)	1096, 1153, 1246, 1513, 2250
TseFI	GTSAC	3 cut(s)	925, 1952, 2036
TseI	GCWGC	5 cut(s)	400, 914, 917, 1119, 2337
Tsp45I	GTSAC	3 cut(s)	925, 1952, 2036
TspGWI	ACGGA	3 cut(s)	573, 1680, 1862
TspRI	CASTG	5 cut(s)	1096, 1153, 1246, 1513, 2250
Van91I	CCANNNNNTGG	1 cut(s)	753
VpaK11BI	GGWCC	3 cut(s)	353, 756, 1703
XagI	CCTNNNNNAGG	2 cut(s)	1361, 2372
XapI	RAATTY	6 cut(s)	463, 616, 1066, 1695, 2188, 2423
XceI	RCATGY	3 cut(s)	699, 883, 2259
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2315
XmiI	GTMKAC	3 cut(s)	444, 1034, 1575
XspI	CTAG	3 cut(s)	215, 1419, 2292
Zsp2I	ATGCAT	2 cut(s)	1528, 2230

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.