Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0001959

Physical Location & Seq

Genomic Coordinates Reverse (-)

8457315 .. 8462316

Gene Structure 5002 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG102200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2460 bp

ATGCCTCCCTCCAAAAAATTTACGCTTGCCCTTTTGTTTTGTTTTTCCGTGATTCTTCTCATCTCCTACGCGAGTGCCATTAAAGTTTCTCATGATGGAAGATCCATCACCATCGATGGCAAACCAAGAATTCTGCTGTCCGGTTCCATTCACTATCCTCGAAGCACACCAGAGATGTGGCCTGCCTTGATTAAGAAGTCAAAGGAGGGTGGATTAGACGCAATTGAGACCTATGTGTTCTGGAATGCTCATGAACCAGTGCGTCGCCAATATGATTTTACTGGCAACCTAGACCTCATTAAGTTCATCAAAACCGTTCAAGATGCTGGACTATACGTCGTTCTTCGAATCGGTCCGTATGTTTGTGCTGAATGGAACTATGGAGGACTTCCTGTTTGGCTGCACAATTTGCCCGGCTGCAAAATTCGAACTGCAAATGATGTCTTCATGAATGAGATGAAAAATTTCACTACCATGATTGTTGATATGGCCAAAAAAGAGAAGCTTTTTGCATCTCAAGGAGGCCCAATTATTATGGCTCAGATTGAAAATGAATATGGAGATGTGGAATGGTGGTATGGAGATGCTGGTAAAGCATATCGTGACTGGTGTGCAAATTTTGCTGAATCTCTTGATGTTGGTGTTCCATGGATTATGTGCCAACAAAACGATGCCCCAGATCCAATGATAAACACTTGCAATGGCTTCTACTGCGACCAATTTACACCCAGACCCAATTATCCTAAAATGTGGACTGAAAATTGGACAGGATGGTTTAAGAGCTGGGGTGGCTCAGATCCATTCAGGACCGCTGAGGATCTTGCTTTCGCAGTTGCAAAATTTTTCCAATTTGGAGGCACATTTCAGAACTATTACATGTATCATGGTGGTACAAACTTCGGTCGAACAGCTGCTGGATACATCACCACTTCTTATGATTACAATGCTCCTCTCGATGAATATGGTACTTTGAATCAACCAAAATGGGGGCATTTGAAGCAACTTCATGAGACTTTGAAATCCATGGAGTACACTCTTACCCATGGAAATATTACCACCGCAGATTTGGGTAACTATGTTTCGACCACTGTGTATGTTGCAGAAGAGTCGTCGAGTTGCTTTCTGGGCAATTCAAATTACAAACTTGATGCAAACATCACTTTCCGAGGAACCCAATACACTGTTCCGGCGTGGTCTGTTAGTATTCTTCCTGATTGCAAAATTGAAGTATATAATACTGCTAAGGTTAATACCCAAACAACAGTAAAAGTAAAAAGGCCTAACAAGGCTGAAGATGAACCTGAGTCTCTGCAGTGGACATGGAGGCCTGAGAACATAGATGAGACTAAGATTCTTGGAAAAGGTGAAGTTGTGGTAAATCACCTTATGGAGCAAAAAGAGGCTGCAAATGATGATAGTGATTACCTTTGGTACATGACAAGTCTTGATCTCAAGAAAGATGATCCAATTTGGAGTAAGAACATTACACTAAGGATCAATGGAACTGGTTGCATTCTTCATGCTTATGTGAATGGAGAGCATATTGGTTCTGACAATGCTGCTCGTAATTTTGAGAGAAATGTTACACTAAATTATGGAAGAAACATTATTTCTTTGTTGAGTGTCACAACTGGATTTTCGAACTACGGAGGTGGATATGATTTGCAACCTGCTGGAATACTTGGTCCTGTTGTTTTGATTGGACAGAATGGTGATGAAACTGTGATCAAGGATTTGTCAGCACACAAATGGTCATACAAGGTTGGATTACATGGCTTGACAAACAAGCTATTTAGCTCCGAGAAAGCAACGAAATGGTCGTCTGAAAATTTACCAGTAAACACGATGATGACATGGTACAAGACTACTTTCAAGGCTCCTGTAGGAACTGACCCCGTTGTGGTGGATTTACAAGGGTTGGGGAAGGGTGATGCTTGGGTGAATGGTCATAGTATTGGTCGATATTGGCCAAGTTACTTGGGTAAGACCGACGGTTGTAGTGATGCTTGCGACTATCGTGGTAAATATGAGAGTAAAAAATGTACTTCCAACTGTGGCGAACCTACTCAAAGATGGTATCACGTTCCGCGATCTTTCATGCGAGGCGATGATGAGAACACGTTGATCTTGTTCGAAGAATTTGGTGGCAATCCATCTCTTGTAAATTTCCAAACCATCACAGTCGGAAGTGTTTGTGCGAATTCATATGAGAAGAATACCTTGGAATTGGCATGCCAAGGTCGCCCCATTTCAGCAATCAAATTTGCTAGTTTTGGCAACCCTCAAGGTACTTGTGGATCATTCAAGAAAGGCACCTGTGATGGCGCAAATGCACTGTCCATCATTGAAAGGGAATGCATCGGTAAAGAAAAATGCTCGATCAACGTCTCCAACGACAAATTTGACACAACAAATTGTGGAGATATTGTGAAAAGGCTTGCAGTGGAGGCTGTTTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BetaGal_dom4_5 Gal_Lectin Glyco_hydro_35

Protein Analysis

819

Amino Acids

91.63

Weight (kDa)

5.99

Isoelectric Point (pI)

32.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	35 - 338	6.8e-118	Glycosyl hydrolases family 35
Glyco_hydro_42	PF02449	57 - 206	5.5e-07	Beta-galactosidase
GHD	PF17834	363 - 416	7.5e-22	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	617 - 702	5.6e-23	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	744 - 819	1.8e-18	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001959)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31740 AT2G04060 AT5G20710 AT5G20710
fragaria_vesca	FvH4_1g09120 FvH4_1g09190 FvH4_1g09190 FvH4_1g26380
malus_domestica	MD02G1096400.v1.1 MD03G1276000.v1.1 MD15G1220900.v1.1 MD15G1221000.v1.1
prunus_persica	Prupe.7G194500_v2.0.a1
pyrus_communis	pycom02g07640
rosa_chinensis	RchiOBHm_Chr2g0095511 RchiOBHm_Chr2g0095591 RchiOBHm_Chr5g0030701
rosa_laevigata	RLG00000016574 RLG00000016582 RLG00000016583
rosa_multiflora	Rmu_co8462209.1_g000001 Rmu_sc0003238.1_g000025 Rmu_sc0003238.1_g000028 Rmu_sc0004719.1_g000009
rosa_roxburghii	Rroxscaffold_2G00146240
rosa_rugosa	Rorug02G0051800 Rorug02G0052600 Rorug05G0120200
rosa_samantha	Rh2AG099800 Rh2AG100400 Rh2BG101100 Rh2BG102200 Rh2CG103300 Rh2CG104600 Rh2DG100700 Rh2DG101500 Rh5AG211800 Rh5BG210700 Rh5CG234400 Rh5CG234500 Rh5DG216100
rosa_wichuraiana	Rw2G007570 Rw2G007630 Rw2G007640 Rw5G019420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1678
AccB1I	GGYRCC	1 cut(s)	2312
AccII	CGCG	2 cut(s)	71, 2089
AciI	CCGC	3 cut(s)	810, 1059, 2087
AclWI	GGATC	7 cut(s)	96, 674, 791, 825, 1457, 1502, 2305
AcoI	YGGCCR	2 cut(s)	489, 1967
AcuI	CTGAAG	1 cut(s)	1311
AfaI	GTAC	7 cut(s)	892, 967, 1031, 1433, 1859, 2044, 2290
AfiI	CCNNNNNNNGG	2 cut(s)	986, 1900
AflIII	ACRYGT	2 cut(s)	876, 2118
AhdI	GACNNNNNGTC	1 cut(s)	335
AjuI	GAANNNNNNNTTGG	4 cut(s)	1167, 1199, 2204, 2236
AluBI	AGCT	5 cut(s)	505, 783, 911, 1789, 1797
AluI	AGCT	5 cut(s)	505, 783, 911, 1789, 1797
Alw26I	GTCTC	5 cut(s)	221, 1004, 1311, 1337, 2392
AlwI	GGATC	7 cut(s)	96, 674, 791, 825, 1457, 1502, 2305
AlwNI	CAGNNNCTG	1 cut(s)	914
AoxI	GGCC	6 cut(s)	179, 489, 523, 1277, 1325, 1967
ApeKI	GCWGC	5 cut(s)	400, 417, 911, 1403, 1559
ArsI	GACNNNNNNTTYG	6 cut(s)	378, 410, 1805, 1837, 2321, 2353
Asp700I	GAANNNNTTC	1 cut(s)	464
AspLEI	GCGC	1 cut(s)	2327
AspS9I	GGNCC	4 cut(s)	353, 524, 807, 1685
AsuC2I	CCSGG	1 cut(s)	414
AsuHPI	GGTGA	7 cut(s)	100, 916, 1373, 1376, 1724, 1940, 1951
AsuII	TTCGAA	4 cut(s)	346, 427, 1640, 2133
AvaII	GGWCC	3 cut(s)	353, 807, 1685
BalI	TGGCCA	2 cut(s)	491, 1969
BanI	GGYRCC	1 cut(s)	2312
BbsI	GAAGAC	1 cut(s)	436
BbvCI	CCTCAGC	1 cut(s)	813
BbvI	GCAGC	5 cut(s)	387, 404, 898, 1390, 1546
BcgI	CGANNNNNNTGC	2 cut(s)	1089, 1123
BciVI	GTATCC	1 cut(s)	911
BclI	TGATCA	1 cut(s)	1725
BcnI	CCSGG	1 cut(s)	414
BcoDI	GTCTC	5 cut(s)	221, 1004, 1311, 1337, 2392
BfaI	CTAG	2 cut(s)	290, 2268
BfmI	CTRYAG	2 cut(s)	1310, 1881
BfuAI	ACCTGC	1 cut(s)	1678
BfuI	GTATCC	1 cut(s)	911
BisI	GCNGC	5 cut(s)	401, 418, 912, 1404, 1560
BlsI	GCNGC	5 cut(s)	402, 419, 913, 1405, 1561
Bme1390I	CCNGG	1 cut(s)	414
Bme18I	GGWCC	3 cut(s)	353, 807, 1685
BmeRI	GACNNNNNGTC	1 cut(s)	335
BmgT120I	GGNCC	4 cut(s)	353, 524, 807, 1685
BmiI	GGNNCC	4 cut(s)	145, 1171, 1878, 2314
BmrFI	CCNGG	1 cut(s)	414
BmsI	GCATC	8 cut(s)	313, 521, 574, 661, 1138, 1921, 1993, 2367
BpiI	GAAGAC	1 cut(s)	436
Bpu10I	CCTNAGC	2 cut(s)	813, 1242
Bpu14I	TTCGAA	4 cut(s)	346, 427, 1640, 2133
BpuEI	CTTGAG	3 cut(s)	501, 1436, 2268
BpuMI	CCSGG	1 cut(s)	414
Bsa29I	ATCGAT	1 cut(s)	114
BsaI	GGTCTC	1 cut(s)	221
BsaJI	CCNNGG	6 cut(s)	647, 1023, 1042, 1165, 2220, 2236
BsaWI	WCCGGW	1 cut(s)	140
BsaXI	ACNNNNNCTCC	2 cut(s)	2412, 2442
Bsc4I	CCNNNNNNNGG	2 cut(s)	986, 1900
Bse1I	ACTGG	6 cut(s)	257, 286, 611, 1510, 1636, 1835
Bse3DI	GCAATG	1 cut(s)	706
BseCI	ATCGAT	1 cut(s)	114
BseDI	CCNNGG	6 cut(s)	647, 1023, 1042, 1165, 2220, 2236
BseGI	GGATG	1 cut(s)	776
BseLI	CCNNNNNNNGG	2 cut(s)	986, 1900
BseMI	GCAATG	1 cut(s)	706
BseMII	CTCAG	5 cut(s)	554, 804, 807, 1293, 1320
BseNI	ACTGG	6 cut(s)	257, 286, 611, 1510, 1636, 1835
BseRI	GAGGAG	1 cut(s)	939
BseXI	GCAGC	5 cut(s)	387, 404, 898, 1390, 1546
BseYI	CCCAGC	1 cut(s)	783
BsgI	GTGCAG	1 cut(s)	386
Bsh1236I	CGCG	2 cut(s)	71, 2089
Bsh1285I	CGRYCG	1 cut(s)	904
BshFI	GGCC	6 cut(s)	181, 491, 525, 1279, 1327, 1969
BshNI	GGYRCC	1 cut(s)	2312
BshVI	ATCGAT	1 cut(s)	114
BsiEI	CGRYCG	1 cut(s)	904
BsiSI	CCGG	3 cut(s)	141, 414, 1187
BslI	CCNNNNNNNGG	2 cut(s)	986, 1900
BsmAI	GTCTC	5 cut(s)	221, 1004, 1311, 1337, 2392
BsmBI	CGTCTC	1 cut(s)	2392
BsmI	GAATGC	3 cut(s)	250, 1512, 2360
BsnI	GGCC	6 cut(s)	181, 491, 525, 1279, 1327, 1969
Bso31I	GGTCTC	1 cut(s)	221
Bsp119I	TTCGAA	4 cut(s)	346, 427, 1640, 2133
Bsp19I	CCATGG	3 cut(s)	647, 1023, 1042
BspACI	CCGC	3 cut(s)	810, 1059, 2087
BspANI	GGCC	6 cut(s)	181, 491, 525, 1279, 1327, 1969
BspCNI	CTCAG	5 cut(s)	553, 805, 806, 1294, 1321
BspDI	ATCGAT	1 cut(s)	114
BspFNI	CGCG	2 cut(s)	71, 2089
BspHI	TCATGA	4 cut(s)	91, 250, 447, 1006
BspLI	GGNNCC	4 cut(s)	145, 1171, 1878, 2314
BspMAI	CTGCAG	1 cut(s)	1314
BspMI	ACCTGC	1 cut(s)	1678
BspPI	GGATC	7 cut(s)	96, 674, 791, 825, 1457, 1502, 2305
BspT104I	TTCGAA	4 cut(s)	346, 427, 1640, 2133
BspT107I	GGYRCC	1 cut(s)	2312
BspTNI	GGTCTC	1 cut(s)	221
BsrDI	GCAATG	1 cut(s)	706
BsrI	ACTGG	6 cut(s)	257, 286, 611, 1510, 1636, 1835
BssECI	CCNNGG	6 cut(s)	647, 1023, 1042, 1165, 2220, 2236
BssT1I	CCWWGG	5 cut(s)	647, 1023, 1042, 2220, 2236
Bst4CI	ACNGT	9 cut(s)	316, 1090, 1183, 1264, 1723, 1994, 2054, 2182, 2337
Bst6I	CTCTTC	1 cut(s)	1098
BstAPI	GCANNNNNTGC	2 cut(s)	409, 620
BstBI	TTCGAA	4 cut(s)	346, 427, 1640, 2133
BstC8I	GCNNGC	5 cut(s)	27, 183, 2008, 2233, 2439
BstDEI	CTNAG	8 cut(s)	540, 793, 813, 1242, 1302, 1329, 1347, 1490
BstDSI	CCRYGG	3 cut(s)	647, 1023, 1042
BstF5I	GGATG	1 cut(s)	776
BstFNI	CGCG	2 cut(s)	71, 2089
BstHHI	GCGC	1 cut(s)	2327
BstMAI	GTCTC	5 cut(s)	221, 1004, 1311, 1337, 2392
BstMCI	CGRYCG	1 cut(s)	904
BstMWI	GCNNNNNNNGC	9 cut(s)	409, 593, 620, 711, 789, 997, 1125, 2241, 2447
BstNSI	RCATGY	2 cut(s)	880, 2235
BstSCI	CCNGG	1 cut(s)	412
BstSFI	CTRYAG	2 cut(s)	1310, 1881
BstUI	CGCG	2 cut(s)	71, 2089
BstV1I	GCAGC	5 cut(s)	387, 404, 898, 1390, 1546
BstV2I	GAAGAC	1 cut(s)	436
BstX2I	RGATCY	4 cut(s)	101, 679, 796, 817
BstXI	CCANNNNNNTGG	1 cut(s)	177
BstYI	RGATCY	4 cut(s)	101, 679, 796, 817
Bsu15I	ATCGAT	1 cut(s)	114
BsuI	GTATCC	1 cut(s)	911
BsuRI	GGCC	6 cut(s)	181, 491, 525, 1279, 1327, 1969
BsuTUI	ATCGAT	1 cut(s)	114
BtgI	CCRYGG	3 cut(s)	647, 1023, 1042
BtgZI	GCGATG	1 cut(s)	2121
BtsCI	GGATG	1 cut(s)	776
BtsI	GCAGTG	2 cut(s)	1319, 2448
BtsIMutI	CAGTG	6 cut(s)	264, 1086, 1179, 1319, 2333, 2448
BveI	ACCTGC	1 cut(s)	1678
Cac8I	GCNNGC	5 cut(s)	27, 183, 2008, 2233, 2439
CaiI	CAGNNNCTG	1 cut(s)	914
CciI	TCATGA	4 cut(s)	91, 250, 447, 1006
CfoI	GCGC	1 cut(s)	2327
Cfr13I	GGNCC	4 cut(s)	353, 524, 807, 1685
ClaI	ATCGAT	1 cut(s)	114
CpoI	CGGWCCG	1 cut(s)	353
CseI	GACGC	2 cut(s)	227, 251
Csp6I	GTAC	7 cut(s)	891, 966, 1030, 1432, 1858, 2043, 2289
CspI	CGGWCCG	1 cut(s)	353
CviQI	GTAC	7 cut(s)	891, 966, 1030, 1432, 1858, 2043, 2289
DdeI	CTNAG	8 cut(s)	540, 793, 813, 1242, 1302, 1329, 1347, 1490
DriI	GACNNNNNGTC	1 cut(s)	335
EaeI	YGGCCR	2 cut(s)	489, 1967
Eam1104I	CTCTTC	1 cut(s)	1098
Eam1105I	GACNNNNNGTC	1 cut(s)	335
EarI	CTCTTC	1 cut(s)	1098
Eco130I	CCWWGG	5 cut(s)	647, 1023, 1042, 2220, 2236
Eco147I	AGGCCT	2 cut(s)	1279, 1327
Eco31I	GGTCTC	1 cut(s)	221
Eco47I	GGWCC	3 cut(s)	353, 807, 1685
Eco57I	CTGAAG	1 cut(s)	1311
EcoRI	GAATTC	2 cut(s)	129, 2200
EcoT14I	CCWWGG	5 cut(s)	647, 1023, 1042, 2220, 2236
EcoT22I	ATGCAT	1 cut(s)	2360
ErhI	CCWWGG	5 cut(s)	647, 1023, 1042, 2220, 2236
Esp3I	CGTCTC	1 cut(s)	2392
FalI	AAGNNNNNCTT	4 cut(s)	489, 521, 2204, 2236
FauNDI	CATATG	1 cut(s)	2206
FbaI	TGATCA	1 cut(s)	1725
Fnu4HI	GCNGC	5 cut(s)	401, 418, 912, 1404, 1560
FokI	GGATG	1 cut(s)	783
Fsp4HI	GCNGC	5 cut(s)	401, 418, 912, 1404, 1560
FspBI	CTAG	2 cut(s)	290, 2268
GlaI	GCGC	1 cut(s)	2326
GluI	GCNGC	5 cut(s)	401, 418, 912, 1404, 1560
GsaI	CCCAGC	1 cut(s)	787
HaeIII	GGCC	6 cut(s)	181, 491, 525, 1279, 1327, 1969
HapII	CCGG	3 cut(s)	141, 414, 1187
HgaI	GACGC	2 cut(s)	227, 251
HhaI	GCGC	1 cut(s)	2327
Hin6I	GCGC	1 cut(s)	2325
HinP1I	GCGC	1 cut(s)	2325
HindIII	AAGCTT	1 cut(s)	503
HinfI	GANTC	7 cut(s)	52, 348, 626, 973, 1106, 1304, 1351
HpaII	CCGG	3 cut(s)	141, 414, 1187
HphI	GGTGA	7 cut(s)	100, 916, 1373, 1376, 1724, 1940, 1951
Hpy166II	GTNNAC	4 cut(s)	753, 1032, 1317, 1840
Hpy188I	TCNGA	8 cut(s)	543, 796, 867, 1166, 1552, 1801, 1825, 2186
Hpy8I	GTNNAC	4 cut(s)	753, 1032, 1317, 1840
Hpy99I	CGWCG	4 cut(s)	267, 341, 1114, 1994
HpyAV	CCTTC	1 cut(s)	1918
HpyCH4III	ACNGT	9 cut(s)	316, 1090, 1183, 1264, 1723, 1994, 2054, 2182, 2337
HpyCH4IV	ACGT	4 cut(s)	336, 2082, 2120, 2385
HpyF10VI	GCNNNNNNNGC	9 cut(s)	409, 593, 620, 711, 789, 997, 1125, 2241, 2447
HpyF3I	CTNAG	8 cut(s)	540, 793, 813, 1242, 1302, 1329, 1347, 1490
HpySE526I	ACGT	4 cut(s)	336, 2082, 2120, 2385
HspAI	GCGC	1 cut(s)	2325
Ksp22I	TGATCA	1 cut(s)	1725
LmnI	GCTCC	4 cut(s)	952, 1390, 1802, 1882
Lsp1109I	GCAGC	5 cut(s)	387, 404, 898, 1390, 1546
LweI	GCATC	8 cut(s)	313, 521, 574, 661, 1138, 1921, 1993, 2367
MaeI	CTAG	2 cut(s)	290, 2268
MaeII	ACGT	4 cut(s)	336, 2082, 2120, 2385
MaeIII	GTNAC	5 cut(s)	602, 1070, 1582, 1624, 1973
MfeI	CAATTG	1 cut(s)	222
MflI	RGATCY	4 cut(s)	101, 679, 796, 817
MlsI	TGGCCA	2 cut(s)	491, 1969
MluNI	TGGCCA	2 cut(s)	491, 1969
MlyI	GAGTC	2 cut(s)	1115, 1313
MmeI	TCCRAC	4 cut(s)	1744, 2073, 2164, 2415
Mox20I	TGGCCA	2 cut(s)	491, 1969
Mph1103I	ATGCAT	1 cut(s)	2360
MroXI	GAANNNNTTC	1 cut(s)	464
MscI	TGGCCA	2 cut(s)	491, 1969
MseI	TTAA	6 cut(s)	81, 192, 300, 777, 1248, 2458
MslI	CAYNNNNRTG	4 cut(s)	473, 1524, 1747, 1847
Msp20I	TGGCCA	2 cut(s)	491, 1969
MspA1I	CMGCKG	2 cut(s)	812, 911
MspI	CCGG	3 cut(s)	141, 414, 1187
MspR9I	CCNGG	1 cut(s)	414
MunI	CAATTG	1 cut(s)	222
Mva1269I	GAATGC	3 cut(s)	250, 1512, 2360
MvnI	CGCG	2 cut(s)	71, 2089
MwoI	GCNNNNNNNGC	9 cut(s)	409, 593, 620, 711, 789, 997, 1125, 2241, 2447
NciI	CCSGG	1 cut(s)	414
NcoI	CCATGG	3 cut(s)	647, 1023, 1042
NdeI	CATATG	1 cut(s)	2206
NlaIV	GGNNCC	4 cut(s)	145, 1171, 1878, 2314
NmuCI	GTSAC	2 cut(s)	602, 1624
NsiI	ATGCAT	1 cut(s)	2360
NspI	RCATGY	2 cut(s)	880, 2235
NspV	TTCGAA	4 cut(s)	346, 427, 1640, 2133
PaeI	GCATGC	1 cut(s)	2235
PagI	TCATGA	4 cut(s)	91, 250, 447, 1006
PceI	AGGCCT	2 cut(s)	1279, 1327
PciI	ACATGT	1 cut(s)	876
PcsI	WCGNNNNNNNCGW	2 cut(s)	1817, 2391
PctI	GAATGC	3 cut(s)	250, 1512, 2360
PdmI	GAANNNNTTC	1 cut(s)	464
PfeI	GAWTC	5 cut(s)	52, 348, 626, 973, 1351
PkrI	GCNGC	5 cut(s)	402, 419, 913, 1405, 1561
PleI	GAGTC	2 cut(s)	1114, 1312
PpsI	GAGTC	2 cut(s)	1114, 1312
PscI	ACATGT	1 cut(s)	876
PspFI	CCCAGC	1 cut(s)	783
PspN4I	GGNNCC	4 cut(s)	145, 1171, 1878, 2314
PspPI	GGNCC	4 cut(s)	353, 524, 807, 1685
PstI	CTGCAG	1 cut(s)	1314
PstNI	CAGNNNCTG	1 cut(s)	914
PsuI	RGATCY	4 cut(s)	101, 679, 796, 817
PvuII	CAGCTG	1 cut(s)	911
RsaI	GTAC	7 cut(s)	892, 967, 1031, 1433, 1859, 2044, 2290
RsaNI	GTAC	7 cut(s)	891, 966, 1030, 1432, 1858, 2043, 2289
RseI	CAYNNNNRTG	4 cut(s)	473, 1524, 1747, 1847
Rsr2I	CGGWCCG	1 cut(s)	353
RsrII	CGGWCCG	1 cut(s)	353
SaqAI	TTAA	6 cut(s)	81, 192, 300, 777, 1248, 2458
SatI	GCNGC	5 cut(s)	401, 418, 912, 1404, 1560
Sau96I	GGNCC	4 cut(s)	353, 524, 807, 1685
SchI	GAGTC	2 cut(s)	1115, 1313
ScrFI	CCNGG	1 cut(s)	414
SfaNI	GCATC	8 cut(s)	313, 521, 574, 661, 1138, 1921, 1993, 2367
SfcI	CTRYAG	2 cut(s)	1310, 1881
SfuI	TTCGAA	4 cut(s)	346, 427, 1640, 2133
SinI	GGWCC	3 cut(s)	353, 807, 1685
SmiMI	CAYNNNNRTG	4 cut(s)	473, 1524, 1747, 1847
SmlI	CTYRAG	3 cut(s)	516, 1451, 2283
SmoI	CTYRAG	3 cut(s)	516, 1451, 2283
SphI	GCATGC	1 cut(s)	2235
SseBI	AGGCCT	2 cut(s)	1279, 1327
SsiI	CCGC	3 cut(s)	810, 1059, 2087
SspI	AATATT	1 cut(s)	1051
SspMI	CTAG	2 cut(s)	290, 2268
StuI	AGGCCT	2 cut(s)	1279, 1327
StyD4I	CCNGG	1 cut(s)	412
StyI	CCWWGG	5 cut(s)	647, 1023, 1042, 2220, 2236
TaaI	ACNGT	9 cut(s)	316, 1090, 1183, 1264, 1723, 1994, 2054, 2182, 2337
TaiI	ACGT	4 cut(s)	339, 2085, 2123, 2388
TaqII	GACCGA	3 cut(s)	341, 890, 2003
TatI	WGTACW	2 cut(s)	1029, 2042
TfiI	GAWTC	5 cut(s)	52, 348, 626, 973, 1351
Tru1I	TTAA	6 cut(s)	81, 192, 300, 777, 1248, 2458
Tru9I	TTAA	6 cut(s)	81, 192, 300, 777, 1248, 2458
TscAI	CASTG	6 cut(s)	264, 1093, 1186, 1319, 2340, 2448
TseFI	GTSAC	2 cut(s)	602, 1624
TseI	GCWGC	5 cut(s)	400, 417, 911, 1403, 1559
Tsp45I	GTSAC	2 cut(s)	602, 1624
TspGWI	ACGGA	3 cut(s)	37, 345, 1662
TspRI	CASTG	6 cut(s)	264, 1093, 1186, 1319, 2340, 2448
VpaK11BI	GGWCC	3 cut(s)	353, 807, 1685
XceI	RCATGY	2 cut(s)	880, 2235
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1063
XmnI	GAANNNNTTC	1 cut(s)	464
XspI	CTAG	2 cut(s)	290, 2268
Zsp2I	ATGCAT	1 cut(s)	2360

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.