Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0001959

Physical Location & Seq

Genomic Coordinates Forward (+)

8441533 .. 8445531

Gene Structure 3999 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG099800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2484 bp

ATGGCACTCCCCAAGAGTTTTGCGTTTTCTCTTTGTTTCTGTCTTTCTGTGATCCTCTGCAGCCTCGTTGCTAATGCTACTGACGTTTCTTATGATGGCAGAGCCATCACCATTGATGGCAAACGAAGGATTTTGAATTCTGGTTCCATACATTATCCTCGAAGTACACCACAAATGTGGCCAGACTTGATTAGGAAGTCAAAGGAAGGTGGACTCGATGCAATTGAGACTTATGTCTTCTGGAATGCCCATGAACCAGTTCGTCGTGAATACGATTTTACTGGTAATCTTGACCTGGTTAGGTTTATCAAAACGATTCAAGATGAAGGACTTTATGCTGTTCTTCGGATTGGTCCATATGTTTGTGCTGAATGGAATTATGGAGGACTTCCTGTTTGGCTCCATAACCTGCCAAACTGTCAAATTCGAACAGCAAACGATGTTTTCATGAATGAGATGAAGAATTTCACTACATTGATCGTGGATATGATGAAAAAAGAGAAACTATTTGCATCTCAAGGAGGCCCTATCATTATTGCTCAGATTGAAAATGAATATGGGAATGTGGAATCATACTATGGAGATGCTGGAAAAGCATACATTAACTGGTCTGCAAACTTTGCTGAATCACTAAACATCGGAGTTCCATGGATTATGTGTCAACAAAAAGATGCTCCCGCATCAATGATAAACACATGTAATGGCTGGTATTGTGACACATTCAAGCCAAGTAACCCCAACACTCCTAAGATGTGGACTGAAAATTGGACTGGCTGGTTCAAGAGCTGGGGTGGTTTAGACCCACTCAGGACTGCTGAGGACGTTGCCTTTGCAGTAGCAAGGTTTTTCCAATATGGAGGCACATTTCAAAACTATTACATGTATCATGGTGGAACAAACTTTGGTAGAACAGCAGCTTCATACATTACCACCTCGTATGATTATGATGCCCCTCTTGATGAGTATGGCCATCTGAGTCAACCAAAATGGGGGCATTTGAAAGAGCTTCATGAGTTTTTGAAATCCATGGAATATACTCTCACCTATGGCAATATTACCACCACAGAATTGGGTAACTCTGTCTCAACCACTGTGTTTGCGACAAATGAGTTAAGTAGCTGCATTTTTGGCAATGCAAATAGCAGTTCAGATGCTACAATCACTTTCCAAGGAAACACATACACCATTCCTGCTTGGTCTGTTAGTATTCTTCCTGATTGCAAAAAAGAAGCATACAACACTGCTAAGGTAAACACCCAAACTACAGTGAAAGTAAAAAGTGTCAACAAGGCTGAGGATGAACCTGAATCCCTTCATTGGTCATGGAGGCCCGAGAAGACGGATGACACCCAGCTTCTTGGAAAAGGTGAAGTTGTGGCAAGTCATCTTATTGAACAAAAAGAGGCTGCAAATGATGCTAGTGATTATCTTTGGTACATGACAAGTGTTCAACTCAGTAAGGATGATCCAATTTTGAGTGGGAACATGACATTGAGGATAAATGAAACTGGTTACATTCTTCATGCTTATGTTAATGGAGAACATGTTGGTTCTCAATGGGCTGCTTATAACGTCTTCAACTACGTTTTCGAGAAACCTATCAAGTTGGTTCCCGGAGTGAACACAATTTCATTGCTCAGTGCTACTGTTGGATTTCCGAATTATGGAGGTGGATTTGAGGACATCAAAACTGGAGTAGCCGGTCCTGTTCAATTGGTTGGACAAAGTGGAGATGAAACTGTGATCAAGGACTTGTCAGAACACAAATGGTCATACAAGGTTGGACTCCATGGCTTGAAAGACGAGCTCTTTAGCTCTGCTTCTCGTTTTGCAAATAAATGGTCAGTTGAAGGTTTACCAGTAAACAGAATGATGACATGGTATAAGACAACTTTCAAAGCTCCCCTAGGAACTGAACCAGTTGTCGTGGACTTACACGGGTTGGGTAAGGGATATGCTTGGGTGAACGGGCATAGCATTGGGCGATATTGGCCAAGCTACTTGGCTCCAGAGGATGGTTGTAGTGTTGAAGCTTGTGACTACCGTGGTGCATACGACAATAACAAGTGTGTCTTCAACTGTGGCAAACCTACTCAAAGATGGTATCATGTTCCGCGATCCTTTTTGCAAGATGATGAGAACACATTGGTCTTGTTTGAGGAATTTGGTGGCAACCCATCTTATGTGAATTTCCAGACAATCAGAGTTGGAACTATTTGTGCAAATGCATATGAGAATCATACATTGGAATTGTCATGCCAAGGTCGCCCCATTTCAGCAATCAAATTTGCTAGCTTTGGAAAACCTGAAGGGACTTGTGGATCATTTAAGAATGGCACCTGTGATGGCACAACTGCTTTGTCCATCCTTCAAAAGGAATGTGTTGGAAAAGAGTCGTGCTCAATTGATGTATCAGAAAAGAAGTTCGGTTCCACAAATTGTGGCGATATTGTGAAGAGGCTGGCCGTGGAAGCAGTTTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BetaGal_dom4_5 Gal_Lectin Glyco_hydro_35

Protein Analysis

827

Amino Acids

92.17

Weight (kDa)

5.56

Isoelectric Point (pI)

31.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	36 - 338	1e-118	Glycosyl hydrolases family 35
Glyco_hydro_42	PF02449	57 - 206	8.4e-07	Beta-galactosidase
GHD	PF17834	363 - 417	3.8e-21	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	625 - 714	2.1e-24	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	751 - 827	2.2e-18	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001959)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31740 AT2G04060 AT5G20710 AT5G20710
fragaria_vesca	FvH4_1g09120 FvH4_1g09190 FvH4_1g09190 FvH4_1g26380
malus_domestica	MD02G1096400.v1.1 MD03G1276000.v1.1 MD15G1220900.v1.1 MD15G1221000.v1.1
prunus_persica	Prupe.7G194500_v2.0.a1
pyrus_communis	pycom02g07640
rosa_chinensis	RchiOBHm_Chr2g0095511 RchiOBHm_Chr2g0095591 RchiOBHm_Chr5g0030701
rosa_laevigata	RLG00000016574 RLG00000016582 RLG00000016583
rosa_multiflora	Rmu_co8462209.1_g000001 Rmu_sc0003238.1_g000025 Rmu_sc0003238.1_g000028 Rmu_sc0004719.1_g000009
rosa_roxburghii	Rroxscaffold_2G00146240
rosa_rugosa	Rorug02G0051800 Rorug02G0052600 Rorug05G0120200
rosa_samantha	Rh2AG099800 Rh2AG100400 Rh2BG101100 Rh2BG102200 Rh2CG103300 Rh2CG104600 Rh2DG100700 Rh2DG101500 Rh5AG211800 Rh5BG210700 Rh5CG234400 Rh5CG234500 Rh5DG216100
rosa_wichuraiana	Rw2G007570 Rw2G007630 Rw2G007640 Rw5G019420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1569
Acc36I	ACCTGC	1 cut(s)	417
AccB1I	GGYRCC	1 cut(s)	2336
AccB7I	CCANNNNNTGG	1 cut(s)	2015
AccII	CGCG	1 cut(s)	2116
AciI	CCGC	2 cut(s)	678, 2114
AclWI	GGATC	4 cut(s)	46, 1460, 2112, 2329
AcoI	YGGCCR	4 cut(s)	179, 967, 1991, 2463
AcsI	RAATTY	6 cut(s)	136, 423, 463, 2162, 2188, 2285
AcuI	CTGAAG	1 cut(s)	2328
AfaI	GTAC	2 cut(s)	166, 1436
AfiI	CCNNNNNNNGG	5 cut(s)	989, 1317, 1664, 2015, 2374
AflIII	ACRYGT	3 cut(s)	695, 879, 1543
AjnI	CCWGG	1 cut(s)	294
AjuI	GAANNNNNNNTTGG	2 cut(s)	2228, 2260
AleI	CACNNNNGTG	1 cut(s)	175
Alw21I	GWGCWC	2 cut(s)	1809, 2402
Alw26I	GTCTC	2 cut(s)	221, 1087
AlwI	GGATC	4 cut(s)	46, 1460, 2112, 2329
Ama87I	CYCGRG	1 cut(s)	1331
AoxI	GGCC	6 cut(s)	179, 523, 967, 1328, 1991, 2463
ApeKI	GCWGC	5 cut(s)	60, 914, 1119, 1406, 1562
ApoI	RAATTY	6 cut(s)	136, 423, 463, 2162, 2188, 2285
ArsI	GACNNNNNNTTYG	6 cut(s)	378, 410, 812, 844, 1826, 1858
Asp700I	GAANNNNTTC	3 cut(s)	258, 464, 1311
AspA2I	CCTAGG	1 cut(s)	1906
AspS9I	GGNCC	4 cut(s)	353, 524, 1329, 1703
AsuC2I	CCSGG	1 cut(s)	1614
AsuHPI	GGTGA	4 cut(s)	100, 1033, 1379, 1975
AsuII	TTCGAA	1 cut(s)	427
AsuNHI	GCTAGC	1 cut(s)	2291
AvaI	CYCGRG	1 cut(s)	1331
AvaII	GGWCC	2 cut(s)	353, 1703
AvrII	CCTAGG	1 cut(s)	1906
BalI	TGGCCA	3 cut(s)	181, 969, 1993
BanI	GGYRCC	1 cut(s)	2336
BanII	GRGCYC	1 cut(s)	1809
BbsI	GAAGAC	4 cut(s)	229, 1343, 1567, 2065
Bbv12I	GWGCWC	2 cut(s)	1809, 2402
BbvCI	CCTCAGC	2 cut(s)	816, 1293
BbvI	GCAGC	5 cut(s)	72, 926, 1106, 1393, 1549
BccI	CCATC	9 cut(s)	89, 110, 113, 978, 2009, 2094, 2185, 2339, 2372
BceAI	ACGGC	1 cut(s)	2450
BciT130I	CCWGG	1 cut(s)	296
BclI	TGATCA	1 cut(s)	1743
BcnI	CCSGG	1 cut(s)	1614
BcoDI	GTCTC	2 cut(s)	221, 1087
BfaI	CTAG	3 cut(s)	1419, 1907, 2292
BfmI	CTRYAG	2 cut(s)	58, 1263
BfuAI	ACCTGC	1 cut(s)	417
BisI	GCNGC	5 cut(s)	61, 915, 1120, 1407, 1563
BlnI	CCTAGG	1 cut(s)	1906
BlsI	GCNGC	5 cut(s)	62, 916, 1121, 1408, 1564
Bme1390I	CCNGG	2 cut(s)	296, 1614
Bme18I	GGWCC	2 cut(s)	353, 1703
BmeT110I	CYCGRG	1 cut(s)	1331
BmgT120I	GGNCC	4 cut(s)	353, 524, 1329, 1703
BmiI	GGNNCC	6 cut(s)	145, 401, 1611, 2007, 2338, 2431
BmrFI	CCNGG	2 cut(s)	296, 1614
BmsI	GCATC	8 cut(s)	208, 521, 574, 661, 689, 937, 1141, 1405
BmtI	GCTAGC	1 cut(s)	2295
BoxI	GACNNNNGTC	1 cut(s)	233
BpiI	GAAGAC	4 cut(s)	229, 1343, 1567, 2065
BplI	GAGNNNNNCTC	2 cut(s)	2384, 2416
BpmI	CTGGAG	2 cut(s)	1713, 1992
Bpu10I	CCTNAGC	3 cut(s)	816, 1245, 1293
Bpu14I	TTCGAA	1 cut(s)	427
BpuEI	CTTGAG	1 cut(s)	501
BpuMI	CCSGG	1 cut(s)	1614
BsaJI	CCNNGG	8 cut(s)	647, 1026, 1168, 1789, 1906, 2044, 2260, 2466
BsaXI	ACNNNNNCTCC	2 cut(s)	1530, 1560
Bsc4I	CCNNNNNNNGG	5 cut(s)	989, 1317, 1664, 2015, 2374
Bse118I	RCCGGY	1 cut(s)	1700
Bse1I	ACTGG	8 cut(s)	257, 286, 611, 775, 1513, 1696, 1859, 1919
Bse3DI	GCAATG	2 cut(s)	1138, 1631
BseBI	CCWGG	1 cut(s)	296
BseDI	CCNNGG	8 cut(s)	647, 1026, 1168, 1789, 1906, 2044, 2260, 2466
BseGI	GGATG	5 cut(s)	1303, 1348, 1468, 2020, 2364
BseLI	CCNNNNNNNGG	5 cut(s)	989, 1317, 1664, 2015, 2374
BseMI	GCAATG	2 cut(s)	1138, 1631
BseMII	CTCAG	7 cut(s)	554, 807, 820, 965, 1284, 1468, 1651
BseNI	ACTGG	8 cut(s)	257, 286, 611, 775, 1513, 1696, 1859, 1919
BseXI	GCAGC	5 cut(s)	72, 926, 1106, 1393, 1549
BseYI	CCCAGC	2 cut(s)	786, 1350
Bsh1236I	CGCG	1 cut(s)	2116
BshFI	GGCC	6 cut(s)	181, 525, 969, 1330, 1993, 2465
BshNI	GGYRCC	1 cut(s)	2336
BsiHKAI	GWGCWC	2 cut(s)	1809, 2402
BsiHKCI	CYCGRG	1 cut(s)	1331
BsiSI	CCGG	2 cut(s)	1614, 1701
BslFI	GGGAC	1 cut(s)	2326
BslI	CCNNNNNNNGG	5 cut(s)	989, 1317, 1664, 2015, 2374
BsmAI	GTCTC	2 cut(s)	221, 1087
BsmFI	GGGAC	1 cut(s)	2326
BsmI	GAATGC	1 cut(s)	250
BsnI	GGCC	6 cut(s)	181, 525, 969, 1330, 1993, 2465
BsoBI	CYCGRG	1 cut(s)	1331
Bsp119I	TTCGAA	1 cut(s)	427
Bsp1286I	GDGCHC	2 cut(s)	1809, 2402
Bsp143I	GATC	6 cut(s)	51, 477, 1465, 1743, 2117, 2321
Bsp19I	CCATGG	3 cut(s)	647, 1026, 1789
BspACI	CCGC	2 cut(s)	678, 2114
BspANI	GGCC	6 cut(s)	181, 525, 969, 1330, 1993, 2465
BspCNI	CTCAG	7 cut(s)	553, 808, 819, 966, 1285, 1467, 1650
BspFNI	CGCG	1 cut(s)	2116
BspHI	TCATGA	2 cut(s)	447, 1009
BspLI	GGNNCC	6 cut(s)	145, 401, 1611, 2007, 2338, 2431
BspMAI	CTGCAG	1 cut(s)	62
BspMI	ACCTGC	1 cut(s)	417
BspOI	GCTAGC	1 cut(s)	2295
BspPI	GGATC	4 cut(s)	46, 1460, 2112, 2329
BspT104I	TTCGAA	1 cut(s)	427
BspT107I	GGYRCC	1 cut(s)	2336
BsrDI	GCAATG	2 cut(s)	1138, 1631
BsrFI	RCCGGY	1 cut(s)	1700
BsrI	ACTGG	8 cut(s)	257, 286, 611, 775, 1513, 1696, 1859, 1919
BssAI	RCCGGY	1 cut(s)	1700
BssECI	CCNNGG	8 cut(s)	647, 1026, 1168, 1789, 1906, 2044, 2260, 2466
BssMI	GATC	6 cut(s)	51, 477, 1465, 1743, 2117, 2321
BssT1I	CCWWGG	6 cut(s)	647, 1026, 1168, 1789, 1906, 2260
Bst2UI	CCWGG	1 cut(s)	296
Bst4CI	ACNGT	7 cut(s)	419, 1093, 1267, 1648, 1741, 2045, 2081
Bst6I	CTCTTC	1 cut(s)	2450
BstAPI	GCANNNNNTGC	2 cut(s)	620, 1415
BstBI	TTCGAA	1 cut(s)	427
BstC8I	GCNNGC	2 cut(s)	2293, 2463
BstDEI	CTNAG	9 cut(s)	540, 747, 806, 816, 974, 1245, 1293, 1454, 1637
BstDSI	CCRYGG	5 cut(s)	647, 1026, 1789, 2044, 2466
BstENI	CCTNNNNNAGG	1 cut(s)	2372
BstF5I	GGATG	5 cut(s)	1303, 1348, 1468, 2020, 2364
BstFNI	CGCG	1 cut(s)	2116
BstKTI	GATC	6 cut(s)	54, 480, 1468, 1746, 2120, 2324
BstMAI	GTCTC	2 cut(s)	221, 1087
BstMBI	GATC	6 cut(s)	51, 477, 1465, 1743, 2117, 2321
BstMWI	GCNNNNNNNGC	7 cut(s)	593, 620, 1128, 1415, 1990, 2265, 2471
BstNI	CCWGG	1 cut(s)	296
BstNSI	RCATGY	3 cut(s)	699, 883, 1547
BstPAI	GACNNNNGTC	1 cut(s)	233
BstSCI	CCNGG	2 cut(s)	294, 1612
BstSFI	CTRYAG	2 cut(s)	58, 1263
BstUI	CGCG	1 cut(s)	2116
BstV1I	GCAGC	5 cut(s)	72, 926, 1106, 1393, 1549
BstV2I	GAAGAC	4 cut(s)	229, 1343, 1567, 2065
BstXI	CCANNNNNNTGG	3 cut(s)	177, 1069, 1358
BsuRI	GGCC	6 cut(s)	181, 525, 969, 1330, 1993, 2465
BtgI	CCRYGG	5 cut(s)	647, 1026, 1789, 2044, 2466
BtsCI	GGATG	5 cut(s)	1303, 1348, 1468, 2020, 2364
BtsI	GCAGTG	1 cut(s)	1239
BtsIMutI	CAGTG	4 cut(s)	1089, 1239, 1272, 1645
BveI	ACCTGC	1 cut(s)	417
Cac8I	GCNNGC	2 cut(s)	2293, 2463
CciI	TCATGA	2 cut(s)	447, 1009
Cfr10I	RCCGGY	1 cut(s)	1700
Cfr13I	GGNCC	4 cut(s)	353, 524, 1329, 1703
CsiI	ACCWGGT	1 cut(s)	294
Csp6I	GTAC	2 cut(s)	165, 1435
CspCI	CAANNNNNGTGG	2 cut(s)	1078, 1113
CviQI	GTAC	2 cut(s)	165, 1435
DdeI	CTNAG	9 cut(s)	540, 747, 806, 816, 974, 1245, 1293, 1454, 1637
DpnI	GATC	6 cut(s)	53, 479, 1467, 1745, 2119, 2323
DpnII	GATC	6 cut(s)	51, 477, 1465, 1743, 2117, 2321
EaeI	YGGCCR	4 cut(s)	179, 967, 1991, 2463
Eam1104I	CTCTTC	1 cut(s)	2450
EarI	CTCTTC	1 cut(s)	2450
Ecl136II	GAGCTC	1 cut(s)	1807
Eco130I	CCWWGG	6 cut(s)	647, 1026, 1168, 1789, 1906, 2260
Eco24I	GRGCYC	1 cut(s)	1809
Eco47I	GGWCC	2 cut(s)	353, 1703
Eco53kI	GAGCTC	1 cut(s)	1807
Eco57I	CTGAAG	1 cut(s)	2328
Eco88I	CYCGRG	1 cut(s)	1331
EcoICRI	GAGCTC	1 cut(s)	1807
EcoNI	CCTNNNNNAGG	1 cut(s)	2372
EcoO109I	RGGNCCY	1 cut(s)	524
EcoRI	GAATTC	1 cut(s)	136
EcoRII	CCWGG	1 cut(s)	294
EcoT14I	CCWWGG	6 cut(s)	647, 1026, 1168, 1789, 1906, 2260
EcoT22I	ATGCAT	1 cut(s)	2230
EcoT38I	GRGCYC	1 cut(s)	1809
ErhI	CCWWGG	6 cut(s)	647, 1026, 1168, 1789, 1906, 2260
FalI	AAGNNNNNCTT	2 cut(s)	2057, 2089
FaqI	GGGAC	1 cut(s)	2326
FauI	CCCGC	1 cut(s)	685
FauNDI	CATATG	2 cut(s)	358, 2230
FbaI	TGATCA	1 cut(s)	1743
Fnu4HI	GCNGC	5 cut(s)	61, 915, 1120, 1407, 1563
FokI	GGATG	5 cut(s)	1310, 1355, 1475, 2027, 2351
FriOI	GRGCYC	1 cut(s)	1809
Fsp4HI	GCNGC	5 cut(s)	61, 915, 1120, 1407, 1563
FspBI	CTAG	3 cut(s)	1419, 1907, 2292
GluI	GCNGC	5 cut(s)	61, 915, 1120, 1407, 1563
GsaI	CCCAGC	2 cut(s)	790, 1354
GsuI	CTGGAG	2 cut(s)	1713, 1992
HaeIII	GGCC	6 cut(s)	181, 525, 969, 1330, 1993, 2465
HapII	CCGG	2 cut(s)	1614, 1701
HincII	GTYRAC	3 cut(s)	662, 980, 1285
HindII	GTYRAC	3 cut(s)	662, 980, 1285
HindIII	AAGCTT	1 cut(s)	2031
HinfI	GANTC	9 cut(s)	213, 316, 569, 626, 976, 1307, 1785, 2236, 2393
HpaII	CCGG	2 cut(s)	1614, 1701
HphI	GGTGA	4 cut(s)	100, 1033, 1379, 1975
Hpy188I	TCNGA	9 cut(s)	348, 543, 641, 975, 1150, 1659, 1759, 2204, 2416
Hpy99I	CGWCG	1 cut(s)	267
HpyAV	CCTTC	7 cut(s)	120, 200, 320, 1322, 1844, 2303, 2378
HpyCH4III	ACNGT	7 cut(s)	419, 1093, 1267, 1648, 1741, 2045, 2081
HpyCH4IV	ACGT	4 cut(s)	84, 822, 1572, 1584
HpyF10VI	GCNNNNNNNGC	7 cut(s)	593, 620, 1128, 1415, 1990, 2265, 2471
HpyF3I	CTNAG	9 cut(s)	540, 747, 806, 816, 974, 1245, 1293, 1454, 1637
HpySE526I	ACGT	4 cut(s)	84, 822, 1572, 1584
Ksp22I	TGATCA	1 cut(s)	1743
Kzo9I	GATC	6 cut(s)	51, 477, 1465, 1743, 2117, 2321
LmnI	GCTCC	4 cut(s)	405, 679, 1906, 2011
Lsp1109I	GCAGC	5 cut(s)	72, 926, 1106, 1393, 1549
LweI	GCATC	8 cut(s)	208, 521, 574, 661, 689, 937, 1141, 1405
MabI	ACCWGGT	1 cut(s)	294
MaeI	CTAG	3 cut(s)	1419, 1907, 2292
MaeII	ACGT	4 cut(s)	84, 822, 1572, 1584
MaeIII	GTNAC	5 cut(s)	713, 731, 1073, 1511, 2036
MalI	GATC	6 cut(s)	53, 479, 1467, 1745, 2119, 2323
MboI	GATC	6 cut(s)	51, 477, 1465, 1743, 2117, 2321
MboII	GAAGA	9 cut(s)	229, 335, 472, 1202, 1348, 1511, 1567, 2065, 2467
MfeI	CAATTG	3 cut(s)	222, 1712, 2403
MhlI	GDGCHC	2 cut(s)	1809, 2402
MlsI	TGGCCA	3 cut(s)	181, 969, 1993
MluNI	TGGCCA	3 cut(s)	181, 969, 1993
MlyI	GAGTC	4 cut(s)	207, 985, 1779, 2402
MmeI	TCCRAC	5 cut(s)	1630, 1699, 1762, 2188, 2365
Mox20I	TGGCCA	3 cut(s)	181, 969, 1993
Mph1103I	ATGCAT	1 cut(s)	2230
MroXI	GAANNNNTTC	3 cut(s)	258, 464, 1311
MscI	TGGCCA	3 cut(s)	181, 969, 1993
MseI	TTAA	4 cut(s)	603, 1112, 1533, 2328
MslI	CAYNNNNRTG	2 cut(s)	175, 1527
Msp20I	TGGCCA	3 cut(s)	181, 969, 1993
MspI	CCGG	2 cut(s)	1614, 1701
MspR9I	CCNGG	2 cut(s)	296, 1614
MunI	CAATTG	3 cut(s)	222, 1712, 2403
Mva1269I	GAATGC	1 cut(s)	250
MvaI	CCWGG	1 cut(s)	296
MvnI	CGCG	1 cut(s)	2116
MwoI	GCNNNNNNNGC	7 cut(s)	593, 620, 1128, 1415, 1990, 2265, 2471
NciI	CCSGG	1 cut(s)	1614
NcoI	CCATGG	3 cut(s)	647, 1026, 1789
NdeI	CATATG	2 cut(s)	358, 2230
NdeII	GATC	6 cut(s)	51, 477, 1465, 1743, 2117, 2321
NheI	GCTAGC	1 cut(s)	2291
NlaIV	GGNNCC	6 cut(s)	145, 401, 1611, 2007, 2338, 2431
NmuCI	GTSAC	2 cut(s)	713, 2036
NsiI	ATGCAT	1 cut(s)	2230
NspI	RCATGY	3 cut(s)	699, 883, 1547
NspV	TTCGAA	1 cut(s)	427
OliI	CACNNNNGTG	1 cut(s)	175
PagI	TCATGA	2 cut(s)	447, 1009
PciI	ACATGT	3 cut(s)	695, 879, 1543
PctI	GAATGC	1 cut(s)	250
PdmI	GAANNNNTTC	3 cut(s)	258, 464, 1311
PfeI	GAWTC	5 cut(s)	316, 569, 626, 1307, 2236
PflMI	CCANNNNNTGG	1 cut(s)	2015
PfoI	TCCNGGA	1 cut(s)	1612
PkrI	GCNGC	5 cut(s)	62, 916, 1121, 1408, 1564
PleI	GAGTC	4 cut(s)	207, 984, 1779, 2401
PpsI	GAGTC	4 cut(s)	207, 984, 1779, 2401
PscI	ACATGT	3 cut(s)	695, 879, 1543
PshAI	GACNNNNGTC	1 cut(s)	233
PsiI	TTATAA	1 cut(s)	1569
Psp124BI	GAGCTC	1 cut(s)	1809
Psp6I	CCWGG	1 cut(s)	294
PspFI	CCCAGC	2 cut(s)	786, 1350
PspGI	CCWGG	1 cut(s)	294
PspN4I	GGNNCC	6 cut(s)	145, 401, 1611, 2007, 2338, 2431
PspPI	GGNCC	4 cut(s)	353, 524, 1329, 1703
PstI	CTGCAG	1 cut(s)	62
RsaI	GTAC	2 cut(s)	166, 1436
RsaNI	GTAC	2 cut(s)	165, 1435
RseI	CAYNNNNRTG	2 cut(s)	175, 1527
SacI	GAGCTC	1 cut(s)	1809
SaqAI	TTAA	4 cut(s)	603, 1112, 1533, 2328
SatI	GCNGC	5 cut(s)	61, 915, 1120, 1407, 1563
Sau3AI	GATC	6 cut(s)	51, 477, 1465, 1743, 2117, 2321
Sau96I	GGNCC	4 cut(s)	353, 524, 1329, 1703
SchI	GAGTC	4 cut(s)	207, 985, 1779, 2402
ScrFI	CCNGG	2 cut(s)	296, 1614
SduI	GDGCHC	2 cut(s)	1809, 2402
SexAI	ACCWGGT	1 cut(s)	294
SfaNI	GCATC	8 cut(s)	208, 521, 574, 661, 689, 937, 1141, 1405
SfcI	CTRYAG	2 cut(s)	58, 1263
SfuI	TTCGAA	1 cut(s)	427
SinI	GGWCC	2 cut(s)	353, 1703
SmiMI	CAYNNNNRTG	2 cut(s)	175, 1527
SmlI	CTYRAG	1 cut(s)	516
SmoI	CTYRAG	1 cut(s)	516
SsiI	CCGC	2 cut(s)	678, 2114
SspI	AATATT	1 cut(s)	1054
SspMI	CTAG	3 cut(s)	1419, 1907, 2292
SstI	GAGCTC	1 cut(s)	1809
StyD4I	CCNGG	2 cut(s)	294, 1612
StyI	CCWWGG	6 cut(s)	647, 1026, 1168, 1789, 1906, 2260
TaaI	ACNGT	7 cut(s)	419, 1093, 1267, 1648, 1741, 2045, 2081
TaiI	ACGT	4 cut(s)	87, 825, 1575, 1587
TaqI	TCGA	4 cut(s)	160, 216, 427, 1590
TatI	WGTACW	1 cut(s)	164
TfiI	GAWTC	5 cut(s)	316, 569, 626, 1307, 2236
Tru1I	TTAA	4 cut(s)	603, 1112, 1533, 2328
Tru9I	TTAA	4 cut(s)	603, 1112, 1533, 2328
TscAI	CASTG	4 cut(s)	1096, 1246, 1272, 1645
TseFI	GTSAC	2 cut(s)	713, 2036
TseI	GCWGC	5 cut(s)	60, 914, 1119, 1406, 1562
Tsp45I	GTSAC	2 cut(s)	713, 2036
TspGWI	ACGGA	1 cut(s)	1355
TspRI	CASTG	4 cut(s)	1096, 1246, 1272, 1645
Van91I	CCANNNNNTGG	1 cut(s)	2015
VpaK11BI	GGWCC	2 cut(s)	353, 1703
XagI	CCTNNNNNAGG	1 cut(s)	2372
XapI	RAATTY	6 cut(s)	136, 423, 463, 2162, 2188, 2285
XceI	RCATGY	3 cut(s)	699, 883, 1547
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1066
XmaJI	CCTAGG	1 cut(s)	1906
XmnI	GAANNNNTTC	3 cut(s)	258, 464, 1311
XspI	CTAG	3 cut(s)	1419, 1907, 2292
Zsp2I	ATGCAT	1 cut(s)	2230

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.