Rh5BG210700

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001959
Physical Location & Seq
Forward (+)
24441378 .. 24443158
1781 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG210700.1

Sequence Viewer

Length: 1248 bp
ATGCAGAAGGTGCTAAATGAAGCCGATGCTAACGATCCAGAGGAACCTTATGGTTTAGTTTGGGGTTGGAGGGCTGAGCACTTTACACACCTCAAGGAAAATGGATCAGTTCTCCATAGCAATCTCACGACTAATCAACTCCTTGATCAGAAAGTGGTAACAAATGATACCAGTGATTACTTGTGGTACATTACCAGTCTGGATCACAATGCAACTGATCCCAATTGGTCCGGCAAAGAAATTACGCTGAGAGTGAATACCAGTGGACATGTCCTCCATGCTTTTGTTAATGGAAAACACATAGGGACTGAAGTTTCTGGACAACATTTCAATCCCTTCACTTTAGAGAGAAAGATTAAGCTAAAGCATGGAAAGAATGATTTATCTCTACTTAGTGTCACGGTTGGCTTGAAAAATTATGATCCACTCTTTGATGAAGTCAATGTTGGGATTCATGGTCCTGTTCAACTGATTGGCAAGTACAAGAATAGTACTGAGGTGACAAAGGACTTGAGCAAAAATGAGTGGATATATAAGGTTGGACTTGCTGGAGAAGAAAAGGGCCTTTACCAAATTACTGGTCATGCTGCTAATTTCCACTGGCCTACAGATAAACTTCCTACAGATAGAATGTTTGTTTGGTACAAGACAATCTTCAAAGCTCCTCTTGGGACCGATCCAGTTGTGGTGGATTTAATGGGTTTAGGAAAGGGTCATGCTTGGGTGAACGGTCGAAGCATTGGCAGGTATTGGACAAGTCACACTGCAGATGAAAATGGTTGTCCAGCAAAATGCGACTATCGTGGAACTTACAGCAATAAGAAGTGCTTGACTAATTGTGGGAAACCTTCCCAAAGATGGTACCATATACCGCGATCTTTCCTCCAAGATGACAATAATGCACTAGTTCTCTTTGAGGAGTTTGGAGGCCAGCCGTTCAATGTTGAATTTCAGACTGTAACAGTTGCTAAAGCTTGCGCAAATGCTTACGAAGGTAATGTCTTGCACTTGTCATGCCAAGGAGGAAGAGTTTTATCCGATGTTAGATTTTCCAGCTTCGGTGATCCACAAGGCGCTTGTGGGGCATCCTTCACAAAAGGCGAGTGTGAATCCCCCACAGCTTTATCATACATCCAAAAGGCATGCATTGGCAAAGCAGAATGTTTTATCGATGTCTCTGAAATGACTCTTGGACCAACAGGCTGCAGAGACATGAATCGCCTTGCTGTCGAAGTCGATTGTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gal_Lectin Glyco_hydro_35

Protein Analysis

415

Amino Acids

46.15

Weight (kDa)

6.24

Isoelectric Point (pI)

22.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BetaGal_gal-bd PF21467 213 - 300 5.6e-23 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 337 - 414 8.4e-14 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 979
Acc36I ACCTGC 1 cut(s) 735
Acc65I GGTACC 1 cut(s) 861
AccB1I GGYRCC 1 cut(s) 861
AccII CGCG 1 cut(s) 874
AciI CCGC 1 cut(s) 872
AclWI GGATC 7 cut(s) 29, 112, 210, 212, 416, 671, 1058
AcsI RAATTY 1 cut(s) 947
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 5 cut(s) 188, 482, 493, 644, 863
AfiI CCNNNNNNNGG 1 cut(s) 858
AflIII ACRYGT 1 cut(s) 268
AgsI TTSAA 6 cut(s) 331, 412, 467, 658, 940, 947
AhlI ACTAGT 1 cut(s) 904
AjuI GAANNNNNNNTTGG 6 cut(s) 429, 461, 622, 654, 1173, 1205
AluBI AGCT 5 cut(s) 361, 662, 974, 1056, 1121
AluI AGCT 5 cut(s) 361, 662, 974, 1056, 1121
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 2 cut(s) 1180, 1203
AlwI GGATC 7 cut(s) 29, 112, 210, 212, 416, 671, 1058
AoxI GGCC 3 cut(s) 562, 602, 928
ApeKI GCWGC 2 cut(s) 587, 1203
ApoI RAATTY 1 cut(s) 947
ArsI GACNNNNNNTTYG 4 cut(s) 565, 597, 984, 1016
Asp718I GGTACC 1 cut(s) 861
AspLEI GCGC 2 cut(s) 980, 1076
AspS9I GGNCC 5 cut(s) 228, 458, 562, 672, 1193
AsuHPI GGTGA 3 cut(s) 511, 736, 1073
AvaII GGWCC 4 cut(s) 228, 458, 672, 1193
BanI GGYRCC 1 cut(s) 861
Bbv12I GWGCWC 1 cut(s) 81
BbvI GCAGC 2 cut(s) 574, 1190
BccI CCATC 1 cut(s) 852
BceAI ACGGC 1 cut(s) 919
BclI TGATCA 1 cut(s) 145
BcoDI GTCTC 2 cut(s) 1180, 1203
BcuI ACTAGT 1 cut(s) 904
BfaI CTAG 1 cut(s) 905
BfmI CTRYAG 4 cut(s) 606, 621, 765, 1204
BfoI RGCGCY 1 cut(s) 1077
BfuAI ACCTGC 1 cut(s) 735
BisI GCNGC 2 cut(s) 588, 1204
BlpI GCTNAGC 1 cut(s) 75
BlsI GCNGC 2 cut(s) 589, 1205
BmcAI AGTACT 1 cut(s) 493
Bme18I GGWCC 4 cut(s) 228, 458, 672, 1193
BmgT120I GGNCC 5 cut(s) 228, 458, 562, 672, 1193
BmiI GGNNCC 3 cut(s) 45, 673, 863
BmsI GCATC 2 cut(s) 16, 1094
BpmI CTGGAG 1 cut(s) 570
Bpu1102I GCTNAGC 1 cut(s) 75
BpuEI CTTGAG 2 cut(s) 77, 532
Bsa29I ATCGAT 1 cut(s) 1170
BsaJI CCNNGG 1 cut(s) 1018
BsaXI ACNNNNNCTCC 4 cut(s) 258, 288, 1014, 1044
Bsc4I CCNNNNNNNGG 1 cut(s) 858
Bse1I ACTGG 6 cut(s) 171, 195, 261, 583, 605, 680
BseCI ATCGAT 1 cut(s) 1170
BseDI CCNNGG 1 cut(s) 1018
BseGI GGATG 2 cut(s) 1085, 1131
BseLI CCNNNNNNNGG 1 cut(s) 858
BseMII CTCAG 3 cut(s) 66, 239, 486
BseNI ACTGG 6 cut(s) 171, 195, 261, 583, 605, 680
BseRI GAGGAG 2 cut(s) 654, 932
BseXI GCAGC 2 cut(s) 574, 1190
Bsh1236I CGCG 1 cut(s) 874
Bsh1285I CGRYCG 1 cut(s) 733
BshFI GGCC 3 cut(s) 564, 604, 930
BshNI GGYRCC 1 cut(s) 861
BshVI ATCGAT 1 cut(s) 1170
BsiEI CGRYCG 1 cut(s) 733
BsiHKAI GWGCWC 1 cut(s) 81
BsiSI CCGG 1 cut(s) 231
BslFI GGGAC 2 cut(s) 319, 685
BslI CCNNNNNNNGG 1 cut(s) 858
BsmAI GTCTC 2 cut(s) 1180, 1203
BsmFI GGGAC 2 cut(s) 319, 685
BsnI GGCC 3 cut(s) 564, 604, 930
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 9 cut(s) 34, 104, 145, 202, 217, 421, 676, 875, 1063
Bsp1720I GCTNAGC 1 cut(s) 75
BspACI CCGC 1 cut(s) 872
BspANI GGCC 3 cut(s) 564, 604, 930
BspCNI CTCAG 3 cut(s) 67, 240, 487
BspDI ATCGAT 1 cut(s) 1170
BspFNI CGCG 1 cut(s) 874
BspLI GGNNCC 3 cut(s) 45, 673, 863
BspMAI CTGCAG 2 cut(s) 769, 1208
BspMI ACCTGC 1 cut(s) 735
BspPI GGATC 7 cut(s) 29, 112, 210, 212, 416, 671, 1058
BspT107I GGYRCC 1 cut(s) 861
BsrI ACTGG 6 cut(s) 171, 195, 261, 583, 605, 680
BssECI CCNNGG 1 cut(s) 1018
BssMI GATC 9 cut(s) 34, 104, 145, 202, 217, 421, 676, 875, 1063
BssT1I CCWWGG 1 cut(s) 1018
Bst4CI ACNGT 4 cut(s) 403, 731, 958, 964
Bst6I CTCTTC 1 cut(s) 1021
BstAPI GCANNNNNTGC 1 cut(s) 10
BstC8I GCNNGC 3 cut(s) 932, 976, 1144
BstDEI CTNAG 5 cut(s) 75, 248, 392, 495, 1245
BstF5I GGATG 2 cut(s) 1085, 1131
BstFNI CGCG 1 cut(s) 874
BstH2I RGCGCY 1 cut(s) 1077
BstHHI GCGC 2 cut(s) 980, 1076
BstKTI GATC 9 cut(s) 37, 107, 148, 205, 220, 424, 679, 878, 1066
BstMAI GTCTC 2 cut(s) 1180, 1203
BstMBI GATC 9 cut(s) 34, 104, 145, 202, 217, 421, 676, 875, 1063
BstMCI CGRYCG 1 cut(s) 733
BstMWI GCNNNNNNNGC 2 cut(s) 10, 1082
BstNSI RCATGY 2 cut(s) 272, 1146
BstSFI CTRYAG 4 cut(s) 606, 621, 765, 1204
BstUI CGCG 1 cut(s) 874
BstV1I GCAGC 2 cut(s) 574, 1190
BstXI CCANNNNNNTGG 1 cut(s) 578
Bsu15I ATCGAT 1 cut(s) 1170
BsuRI GGCC 3 cut(s) 564, 604, 930
BsuTUI ATCGAT 1 cut(s) 1170
BtsCI GGATG 2 cut(s) 1085, 1131
BtsI GCAGTG 1 cut(s) 762
BtsIMutI CAGTG 4 cut(s) 178, 268, 598, 762
BveI ACCTGC 1 cut(s) 735
Cac8I GCNNGC 3 cut(s) 932, 976, 1144
CfoI GCGC 2 cut(s) 980, 1076
Cfr13I GGNCC 5 cut(s) 228, 458, 562, 672, 1193
ClaI ATCGAT 1 cut(s) 1170
Csp6I GTAC 5 cut(s) 187, 481, 492, 643, 862
CviAII CATG 9 cut(s) 269, 278, 368, 455, 584, 716, 1014, 1143, 1213
CviQI GTAC 5 cut(s) 187, 481, 492, 643, 862
DdeI CTNAG 5 cut(s) 75, 248, 392, 495, 1245
DpnI GATC 9 cut(s) 36, 106, 147, 204, 219, 423, 678, 877, 1065
DpnII GATC 9 cut(s) 34, 104, 145, 202, 217, 421, 676, 875, 1063
Eam1104I CTCTTC 1 cut(s) 1021
EarI CTCTTC 1 cut(s) 1021
Eco130I CCWWGG 1 cut(s) 1018
Eco47I GGWCC 4 cut(s) 228, 458, 672, 1193
Eco57I CTGAAG 1 cut(s) 330
EcoO109I RGGNCCY 1 cut(s) 562
EcoT14I CCWWGG 1 cut(s) 1018
EcoT22I ATGCAT 1 cut(s) 1148
ErhI CCWWGG 1 cut(s) 1018
FaeI CATG 9 cut(s) 272, 281, 371, 458, 587, 719, 1017, 1146, 1216
FalI AAGNNNNNCTT 6 cut(s) 638, 670, 651, 683, 812, 844
FaqI GGGAC 2 cut(s) 319, 685
FatI CATG 9 cut(s) 268, 277, 367, 454, 583, 715, 1013, 1142, 1212
FbaI TGATCA 1 cut(s) 145
Fnu4HI GCNGC 2 cut(s) 588, 1204
FokI GGATG 2 cut(s) 1072, 1118
Fsp4HI GCNGC 2 cut(s) 588, 1204
FspBI CTAG 1 cut(s) 905
FspI TGCGCA 1 cut(s) 979
GlaI GCGC 2 cut(s) 979, 1075
GluI GCNGC 2 cut(s) 588, 1204
GsuI CTGGAG 1 cut(s) 570
HaeII RGCGCY 1 cut(s) 1077
HaeIII GGCC 3 cut(s) 564, 604, 930
HapII CCGG 1 cut(s) 231
HhaI GCGC 2 cut(s) 980, 1076
Hin1II CATG 9 cut(s) 272, 281, 371, 458, 587, 719, 1017, 1146, 1216
Hin6I GCGC 2 cut(s) 978, 1074
HinP1I GCGC 2 cut(s) 978, 1074
HindIII AAGCTT 1 cut(s) 972
HinfI GANTC 4 cut(s) 451, 1109, 1186, 1216
HpaII CCGG 1 cut(s) 231
HphI GGTGA 3 cut(s) 511, 736, 1073
Hpy166II GTNNAC 2 cut(s) 266, 727
Hpy188I TCNGA 4 cut(s) 150, 954, 1039, 1180
Hpy188III TCNNGA 4 cut(s) 38, 127, 200, 318
Hpy8I GTNNAC 2 cut(s) 266, 727
HpyAV CCTTC 4 cut(s) 346, 858, 986, 1099
HpyCH4III ACNGT 4 cut(s) 403, 731, 958, 964
HpyCH4V TGCA 7 cut(s) 4, 212, 767, 902, 1006, 1146, 1206
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 1082
HpyF3I CTNAG 5 cut(s) 75, 248, 392, 495, 1245
Hsp92II CATG 9 cut(s) 272, 281, 371, 458, 587, 719, 1017, 1146, 1216
HspAI GCGC 2 cut(s) 978, 1074
KpnI GGTACC 1 cut(s) 865
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 9 cut(s) 34, 104, 145, 202, 217, 421, 676, 875, 1063
LmnI GCTCC 1 cut(s) 667
Lsp1109I GCAGC 2 cut(s) 574, 1190
LweI GCATC 2 cut(s) 16, 1094
MaeI CTAG 1 cut(s) 905
MaeIII GTNAC 5 cut(s) 157, 397, 499, 758, 958
MalI GATC 9 cut(s) 36, 106, 147, 204, 219, 423, 678, 877, 1065
MboI GATC 9 cut(s) 34, 104, 145, 202, 217, 421, 676, 875, 1063
MboII GAAGA 3 cut(s) 566, 646, 1038
MfeI CAATTG 1 cut(s) 223
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 7 cut(s) 223, 240, 415, 573, 592, 835, 947
MlyI GAGTC 1 cut(s) 1180
MmeI TCCRAC 2 cut(s) 47, 520
Mph1103I ATGCAT 1 cut(s) 1148
MseI TTAA 3 cut(s) 288, 357, 695
MspI CCGG 1 cut(s) 231
MunI CAATTG 1 cut(s) 223
MvnI CGCG 1 cut(s) 874
MwoI GCNNNNNNNGC 2 cut(s) 10, 1082
NdeII GATC 9 cut(s) 34, 104, 145, 202, 217, 421, 676, 875, 1063
NlaIII CATG 9 cut(s) 272, 281, 371, 458, 587, 719, 1017, 1146, 1216
NlaIV GGNNCC 3 cut(s) 45, 673, 863
NmuCI GTSAC 3 cut(s) 397, 499, 758
NsbI TGCGCA 1 cut(s) 979
NsiI ATGCAT 1 cut(s) 1148
NspI RCATGY 2 cut(s) 272, 1146
PaeI GCATGC 1 cut(s) 1146
PciI ACATGT 1 cut(s) 268
PfeI GAWTC 3 cut(s) 451, 1109, 1216
PkrI GCNGC 2 cut(s) 589, 1205
PleI GAGTC 1 cut(s) 1180
PpsI GAGTC 1 cut(s) 1180
PscI ACATGT 1 cut(s) 268
PspN4I GGNNCC 3 cut(s) 45, 673, 863
PspPI GGNCC 5 cut(s) 228, 458, 562, 672, 1193
PstI CTGCAG 2 cut(s) 769, 1208
RsaI GTAC 5 cut(s) 188, 482, 493, 644, 863
RsaNI GTAC 5 cut(s) 187, 481, 492, 643, 862
SaqAI TTAA 3 cut(s) 288, 357, 695
SatI GCNGC 2 cut(s) 588, 1204
Sau3AI GATC 9 cut(s) 34, 104, 145, 202, 217, 421, 676, 875, 1063
Sau96I GGNCC 5 cut(s) 228, 458, 562, 672, 1193
ScaI AGTACT 1 cut(s) 493
SchI GAGTC 1 cut(s) 1180
SduI GDGCHC 1 cut(s) 81
SfaNI GCATC 2 cut(s) 16, 1094
SfcI CTRYAG 4 cut(s) 606, 621, 765, 1204
SinI GGWCC 4 cut(s) 228, 458, 672, 1193
SmlI CTYRAG 2 cut(s) 92, 511
SmoI CTYRAG 2 cut(s) 92, 511
SpeI ACTAGT 1 cut(s) 904
SphI GCATGC 1 cut(s) 1146
Sse9I AATT 7 cut(s) 223, 240, 415, 573, 592, 835, 947
SsiI CCGC 1 cut(s) 872
SspMI CTAG 1 cut(s) 905
StyI CCWWGG 1 cut(s) 1018
TaaI ACNGT 4 cut(s) 403, 731, 958, 964
TaqI TCGA 4 cut(s) 733, 1170, 1230, 1236
TaqII GACCGA 1 cut(s) 689
TasI AATT 7 cut(s) 223, 240, 415, 573, 592, 835, 947
TatI WGTACW 2 cut(s) 480, 491
TfiI GAWTC 3 cut(s) 451, 1109, 1216
Tru1I TTAA 3 cut(s) 288, 357, 695
Tru9I TTAA 3 cut(s) 288, 357, 695
TscAI CASTG 4 cut(s) 178, 268, 605, 769
TseFI GTSAC 3 cut(s) 397, 499, 758
TseI GCWGC 2 cut(s) 587, 1203
Tsp45I GTSAC 3 cut(s) 397, 499, 758
TspDTI ATGAA 5 cut(s) 33, 443, 450, 786, 1229
TspRI CASTG 4 cut(s) 178, 268, 605, 769
VpaK11BI GGWCC 4 cut(s) 228, 458, 672, 1193
XapI RAATTY 1 cut(s) 947
XceI RCATGY 2 cut(s) 272, 1146
XspI CTAG 1 cut(s) 905
ZrmI AGTACT 1 cut(s) 493
Zsp2I ATGCAT 1 cut(s) 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.