Rh5DG216100

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001959
Physical Location & Seq
Forward (+)
24389519 .. 24391083
1565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG216100.1

Sequence Viewer

Length: 837 bp
ATGATACCAGTGATTACTTGTGGTACATTACCAGGGACTGAAGTTTCTGGACAACATTTCAACCCCTTCACTTTAGAGAGAAAGATTAAGCTAAAGCATGGAAAGAATGATTTATCTCTACCTAGTGTCACGGTTGGCTTGAAAAATTATGATCCACTCTTTGATGAAGTCAATGTTGGGATTCATGGTCCTGTTCAACTGATTGGCAAGTACAAGAATGGTACTGAGGTGACAAAGGACTTGAGCAAAAATGAGTGGATATATAAGGTTGGACTTGCTGGAGAAGAAAAGGGCCTTTACCAAATTACTGGTCATGCTGCTAATTTCCACTGGCCTACAGATAAACTTCCAACAGATAGAATGTTTGTTTGGTACAAGACAATCTTAATTCAAAGCTCCTCTTGGGACCGATCCAATTGTGGTGGATTTAATGGGTTTAGGAAAGGGTCATGCTTGGGTGAACGCAATAAGAAGTGCTTGACTAATTGTGGGAAACCTTCCCAAAGATGGTACCATATACCGCGATCTTTCCTCCAAGATGACAATAATGCACTAGTTCTCTTTGAGGAGTTTGGAGGCCAGCCGTTCAATGTTGAATTTCAGACTGTAACAGTTGCTAAAGCTTGCGCAAATGCTTACGAAGGTAATGTCTTGCACTTGTCATGCCAAGGAGGAAGAGTTTTATCCGATGTTAGATTTTCCAGCTTCGGTGATCCACAAGGCGCTTGTGGGGCATCCTTCACAAAAGGCGAGTGTGAATCCCCCACAGCTTTATCATACATCCAAAAGGCATGCATTGGCAAAGCAGAATGTTTTATCGATGGCTCTGAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gal_Lectin Glyco_hydro_35

Protein Analysis

278

Amino Acids

30.71

Weight (kDa)

7.96

Isoelectric Point (pI)

38.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUEL_Lectin PF02140 220 - 274 1.9e-09 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 628
Acc65I GGTACC 1 cut(s) 510
AccB1I GGYRCC 1 cut(s) 510
AccII CGCG 1 cut(s) 523
AciI CCGC 1 cut(s) 521
AclWI GGATC 3 cut(s) 146, 405, 707
AcsI RAATTY 2 cut(s) 596, 830
AcuI CTGAAG 1 cut(s) 60
AfaI GTAC 5 cut(s) 25, 212, 223, 374, 512
AfiI CCNNNNNNNGG 1 cut(s) 507
AgsI TTSAA 6 cut(s) 61, 142, 197, 392, 589, 596
AhlI ACTAGT 1 cut(s) 553
AjnI CCWGG 1 cut(s) 31
AjuI GAANNNNNNNTTGG 4 cut(s) 159, 191, 352, 384
AluBI AGCT 5 cut(s) 91, 396, 623, 705, 770
AluI AGCT 5 cut(s) 91, 396, 623, 705, 770
AlwI GGATC 3 cut(s) 146, 405, 707
AlwNI CAGNNNCTG 1 cut(s) 38
AoxI GGCC 3 cut(s) 292, 332, 577
ApeKI GCWGC 1 cut(s) 317
ApoI RAATTY 2 cut(s) 596, 830
ArsI GACNNNNNNTTYG 4 cut(s) 295, 327, 633, 665
Asp718I GGTACC 1 cut(s) 510
AspLEI GCGC 2 cut(s) 629, 725
AspS9I GGNCC 3 cut(s) 188, 292, 406
AsuHPI GGTGA 3 cut(s) 241, 470, 722
AvaII GGWCC 2 cut(s) 188, 406
BaeI ACNNNNGTAYC 2 cut(s) 213, 246
BanI GGYRCC 1 cut(s) 510
BbvI GCAGC 1 cut(s) 304
BccI CCATC 2 cut(s) 501, 815
BceAI ACGGC 1 cut(s) 568
BciT130I CCWGG 1 cut(s) 33
BcuI ACTAGT 1 cut(s) 553
BfaI CTAG 2 cut(s) 123, 554
BfmI CTRYAG 1 cut(s) 336
BfoI RGCGCY 1 cut(s) 726
BisI GCNGC 1 cut(s) 318
BlsI GCNGC 1 cut(s) 319
Bme1390I CCNGG 1 cut(s) 33
Bme18I GGWCC 2 cut(s) 188, 406
BmgT120I GGNCC 3 cut(s) 188, 292, 406
BmiI GGNNCC 2 cut(s) 407, 512
BmrFI CCNGG 1 cut(s) 33
BmsI GCATC 1 cut(s) 743
BpmI CTGGAG 1 cut(s) 300
BpuEI CTTGAG 1 cut(s) 262
Bsa29I ATCGAT 1 cut(s) 819
BsaJI CCNNGG 2 cut(s) 32, 667
BsaXI ACNNNNNCTCC 2 cut(s) 663, 693
Bsc4I CCNNNNNNNGG 1 cut(s) 507
Bse1I ACTGG 3 cut(s) 8, 313, 335
BseBI CCWGG 1 cut(s) 33
BseCI ATCGAT 1 cut(s) 819
BseDI CCNNGG 2 cut(s) 32, 667
BseGI GGATG 2 cut(s) 734, 780
BseLI CCNNNNNNNGG 1 cut(s) 507
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 3 cut(s) 8, 313, 335
BseRI GAGGAG 2 cut(s) 388, 581
BseXI GCAGC 1 cut(s) 304
Bsh1236I CGCG 1 cut(s) 523
BshFI GGCC 3 cut(s) 294, 334, 579
BshNI GGYRCC 1 cut(s) 510
BshVI ATCGAT 1 cut(s) 819
BslFI GGGAC 2 cut(s) 49, 419
BslI CCNNNNNNNGG 1 cut(s) 507
BsmFI GGGAC 2 cut(s) 49, 419
BsnI GGCC 3 cut(s) 294, 334, 579
Bsp143I GATC 4 cut(s) 151, 410, 524, 712
BspACI CCGC 1 cut(s) 521
BspANI GGCC 3 cut(s) 294, 334, 579
BspCNI CTCAG 1 cut(s) 217
BspDI ATCGAT 1 cut(s) 819
BspFNI CGCG 1 cut(s) 523
BspLI GGNNCC 2 cut(s) 407, 512
BspPI GGATC 3 cut(s) 146, 405, 707
BspT107I GGYRCC 1 cut(s) 510
BsrI ACTGG 3 cut(s) 8, 313, 335
BssECI CCNNGG 2 cut(s) 32, 667
BssMI GATC 4 cut(s) 151, 410, 524, 712
BssT1I CCWWGG 1 cut(s) 667
Bst2UI CCWGG 1 cut(s) 33
Bst4CI ACNGT 3 cut(s) 133, 607, 613
Bst6I CTCTTC 1 cut(s) 670
BstC8I GCNNGC 3 cut(s) 581, 625, 793
BstDEI CTNAG 1 cut(s) 225
BstF5I GGATG 2 cut(s) 734, 780
BstFNI CGCG 1 cut(s) 523
BstH2I RGCGCY 1 cut(s) 726
BstHHI GCGC 2 cut(s) 629, 725
BstKTI GATC 4 cut(s) 154, 413, 527, 715
BstMBI GATC 4 cut(s) 151, 410, 524, 712
BstMWI GCNNNNNNNGC 1 cut(s) 731
BstNI CCWGG 1 cut(s) 33
BstNSI RCATGY 1 cut(s) 795
BstSCI CCNGG 1 cut(s) 31
BstSFI CTRYAG 1 cut(s) 336
BstUI CGCG 1 cut(s) 523
BstV1I GCAGC 1 cut(s) 304
BstXI CCANNNNNNTGG 1 cut(s) 308
Bsu15I ATCGAT 1 cut(s) 819
BsuRI GGCC 3 cut(s) 294, 334, 579
BsuTUI ATCGAT 1 cut(s) 819
BtsCI GGATG 2 cut(s) 734, 780
BtsIMutI CAGTG 2 cut(s) 15, 328
Cac8I GCNNGC 3 cut(s) 581, 625, 793
CaiI CAGNNNCTG 1 cut(s) 38
CfoI GCGC 2 cut(s) 629, 725
Cfr13I GGNCC 3 cut(s) 188, 292, 406
ClaI ATCGAT 1 cut(s) 819
Csp6I GTAC 5 cut(s) 24, 211, 222, 373, 511
CspCI CAANNNNNGTGG 2 cut(s) 403, 438
CviAII CATG 6 cut(s) 98, 185, 314, 450, 663, 792
CviQI GTAC 5 cut(s) 24, 211, 222, 373, 511
DdeI CTNAG 1 cut(s) 225
DpnI GATC 4 cut(s) 153, 412, 526, 714
DpnII GATC 4 cut(s) 151, 410, 524, 712
Eam1104I CTCTTC 1 cut(s) 670
EarI CTCTTC 1 cut(s) 670
Eco130I CCWWGG 1 cut(s) 667
Eco47I GGWCC 2 cut(s) 188, 406
Eco57I CTGAAG 1 cut(s) 60
EcoO109I RGGNCCY 1 cut(s) 292
EcoRI GAATTC 1 cut(s) 830
EcoRII CCWGG 1 cut(s) 31
EcoT14I CCWWGG 1 cut(s) 667
EcoT22I ATGCAT 1 cut(s) 797
ErhI CCWWGG 1 cut(s) 667
FaeI CATG 6 cut(s) 101, 188, 317, 453, 666, 795
FalI AAGNNNNNCTT 6 cut(s) 368, 400, 385, 417, 461, 493
FaqI GGGAC 2 cut(s) 49, 419
FatI CATG 6 cut(s) 97, 184, 313, 449, 662, 791
Fnu4HI GCNGC 1 cut(s) 318
FokI GGATG 2 cut(s) 721, 767
Fsp4HI GCNGC 1 cut(s) 318
FspBI CTAG 2 cut(s) 123, 554
FspI TGCGCA 1 cut(s) 628
GlaI GCGC 2 cut(s) 628, 724
GluI GCNGC 1 cut(s) 318
GsuI CTGGAG 1 cut(s) 300
HaeII RGCGCY 1 cut(s) 726
HaeIII GGCC 3 cut(s) 294, 334, 579
HhaI GCGC 2 cut(s) 629, 725
Hin1II CATG 6 cut(s) 101, 188, 317, 453, 666, 795
Hin6I GCGC 2 cut(s) 627, 723
HinP1I GCGC 2 cut(s) 627, 723
HindIII AAGCTT 1 cut(s) 621
HinfI GANTC 2 cut(s) 181, 758
HphI GGTGA 3 cut(s) 241, 470, 722
Hpy166II GTNNAC 1 cut(s) 461
Hpy188I TCNGA 4 cut(s) 603, 688, 829, 836
Hpy188III TCNNGA 1 cut(s) 48
Hpy8I GTNNAC 1 cut(s) 461
HpyAV CCTTC 4 cut(s) 76, 507, 635, 748
HpyCH4III ACNGT 3 cut(s) 133, 607, 613
HpyCH4V TGCA 3 cut(s) 551, 655, 795
HpyF10VI GCNNNNNNNGC 1 cut(s) 731
HpyF3I CTNAG 1 cut(s) 225
Hsp92II CATG 6 cut(s) 101, 188, 317, 453, 666, 795
HspAI GCGC 2 cut(s) 627, 723
KpnI GGTACC 1 cut(s) 514
Kzo9I GATC 4 cut(s) 151, 410, 524, 712
LmnI GCTCC 1 cut(s) 401
Lsp1109I GCAGC 1 cut(s) 304
LweI GCATC 1 cut(s) 743
MaeI CTAG 2 cut(s) 123, 554
MaeIII GTNAC 3 cut(s) 127, 229, 607
MalI GATC 4 cut(s) 153, 412, 526, 714
MboI GATC 4 cut(s) 151, 410, 524, 712
MboII GAAGA 2 cut(s) 296, 687
MfeI CAATTG 1 cut(s) 415
MluCI AATT 8 cut(s) 145, 303, 322, 387, 415, 484, 596, 830
MmeI TCCRAC 2 cut(s) 250, 374
MnlI CCTC 6 cut(s) 220, 409, 542, 559, 569, 665
Mph1103I ATGCAT 1 cut(s) 797
MseI TTAA 3 cut(s) 87, 386, 429
MspR9I CCNGG 1 cut(s) 33
MunI CAATTG 1 cut(s) 415
MvaI CCWGG 1 cut(s) 33
MvnI CGCG 1 cut(s) 523
MwoI GCNNNNNNNGC 1 cut(s) 731
NdeII GATC 4 cut(s) 151, 410, 524, 712
NlaIII CATG 6 cut(s) 101, 188, 317, 453, 666, 795
NlaIV GGNNCC 2 cut(s) 407, 512
NmuCI GTSAC 2 cut(s) 127, 229
NsbI TGCGCA 1 cut(s) 628
NsiI ATGCAT 1 cut(s) 797
NspI RCATGY 1 cut(s) 795
PaeI GCATGC 1 cut(s) 795
PfeI GAWTC 2 cut(s) 181, 758
PkrI GCNGC 1 cut(s) 319
Psp6I CCWGG 1 cut(s) 31
PspGI CCWGG 1 cut(s) 31
PspN4I GGNNCC 2 cut(s) 407, 512
PspPI GGNCC 3 cut(s) 188, 292, 406
PstNI CAGNNNCTG 1 cut(s) 38
RsaI GTAC 5 cut(s) 25, 212, 223, 374, 512
RsaNI GTAC 5 cut(s) 24, 211, 222, 373, 511
SaqAI TTAA 3 cut(s) 87, 386, 429
SatI GCNGC 1 cut(s) 318
Sau3AI GATC 4 cut(s) 151, 410, 524, 712
Sau96I GGNCC 3 cut(s) 188, 292, 406
ScrFI CCNGG 1 cut(s) 33
SfaNI GCATC 1 cut(s) 743
SfcI CTRYAG 1 cut(s) 336
SinI GGWCC 2 cut(s) 188, 406
SmlI CTYRAG 1 cut(s) 241
SmoI CTYRAG 1 cut(s) 241
SpeI ACTAGT 1 cut(s) 553
SphI GCATGC 1 cut(s) 795
Sse9I AATT 8 cut(s) 145, 303, 322, 387, 415, 484, 596, 830
SsiI CCGC 1 cut(s) 521
SspMI CTAG 2 cut(s) 123, 554
StyD4I CCNGG 1 cut(s) 31
StyI CCWWGG 1 cut(s) 667
TaaI ACNGT 3 cut(s) 133, 607, 613
TaqI TCGA 1 cut(s) 819
TaqII GACCGA 1 cut(s) 423
TasI AATT 8 cut(s) 145, 303, 322, 387, 415, 484, 596, 830
TatI WGTACW 1 cut(s) 210
TfiI GAWTC 2 cut(s) 181, 758
Tru1I TTAA 3 cut(s) 87, 386, 429
Tru9I TTAA 3 cut(s) 87, 386, 429
TscAI CASTG 2 cut(s) 15, 335
TseFI GTSAC 2 cut(s) 127, 229
TseI GCWGC 1 cut(s) 317
Tsp45I GTSAC 2 cut(s) 127, 229
TspDTI ATGAA 2 cut(s) 173, 180
TspRI CASTG 2 cut(s) 15, 335
VpaK11BI GGWCC 2 cut(s) 188, 406
XapI RAATTY 2 cut(s) 596, 830
XceI RCATGY 1 cut(s) 795
XspI CTAG 2 cut(s) 123, 554
Zsp2I ATGCAT 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.