Rh5CG234400

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001959
Physical Location & Seq
Forward (+)
24372883 .. 24377649
4767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG234400.1

Sequence Viewer

Length: 2520 bp
ATGGCAACTATGCATTCATATAGATTTTTGTTTCTTGCTTCTAGTCTCTTACTGTGTCAACTATGTTCTGCTTTGCCGGTGGAGGTCGATCATGATGGAAGAGCCATCACAATCAATGGTGATAGAAGAATCATAATCTCTGGCTCTATTCACTACCCACGTAGTACTCCTGAAATGTGGCCGGACTTGATCCAAAAGGCAAAAGATGGAGGCCTTGATGCCGTTGAGACTTATGTCTTTTGGAATGCACATGAACCTCTGCGTAGGCAGTATGACTTTTCAGGAAACCTGGACCTGGTGAGATTCATTCAGACCATCCAAAAGGCAGGGCTCTATGCTGTTCTTCGGATTGGACCATATGTTTGTGCAGAATGGAATTATGGAGGTTTTCCTGTGTGGCTGCATAACATACCAGGCATCAAATTGAGAACAAACAATGATATCTATAAGAATGAGATGCAAAACTTTACAACTTTGATAGTGGACATGATGAAGCATGAGAATCTTTTTGCTTCACAAGGGGGACCCATTATACTTGCCCAGATTGAAAACGAGTATGGGAATGTTATGCGCTCGTATGGCGATGAAGGAAAGAAATATATACAATGGTGTGCTGATTTAGTTGAATCTTACCAGATTGGTGTACCATGGATTATGTGCCAACAGGAAGATGCTCCACAATCAATGATTAATACCTGCAATGGCTGGTATTGTGATCAATTTAAGCCTTCATACAACACTAGCCCGAAGATGTGGACAGAAAATTGGACTGGATGGTTCAAGGATTGGGGCATGCAAGATCCACACAGAACTGCAGAGGATCTCGCCTTTTCTGTTGCACGATTCTTTCAGTTAAATGGCACCTTCCAAAACTATTATATGTACCATGGAGGAACAAATTTCGGTCGTTCAGCAGGAGGTCCTTACATCACCACATCATATGATTATGATGCACCATTAGATGAATATGGTAATTTGAATCAACCCAAGTGGGGGCATCTTAAAGAGCTTCATAAACATATAAGATCAATGGAGAAAGTTCTGACTTATGGTGACGTAAGACAGGTTGACTATGGAAACAGCTCGAGTGTGACAGAGTACAGCTACAGAGGAAATCGATCCTGTTTCATTAGTAACGCAAACACAACAAGCAATGTAATAGTGAACTTTGAGAATAATAAGTATTCTGTTCCTGCTTGGTCTGTTACTATCCTTCCTGATTGCTATACTGAAGTCTACAACACTGCAAAGGTTAACGTGCAAAGATCAATAATGCAGAAGGTGCTAAATGAAGCCGACGCTAACGATCCAGAGGAACCTTATGGTTTAGTTTGGGGTTGGAGGGCTGAGCACTTTACACACCTCAAGGAAAATGGATCAGTTCTGCATAGCAATCTCACGACTAATCAACTCCTTGATCAGAAAGTAGTAACAAATGATACCAGTGATTACTTGTGGTACATTACCAGTCTGGATCACAATGCAACTGATCCCAATTGGTCCGGCAAAGAAATTACGCTGAGAGTGAATACCAGTGGACATGTCCTCCATGCTTTTGTTAATGGAAAACACATAGGGACTGAAGTTTCTGGACAACATTTCAATCCCTTCACTTTAGAGAGAAAGATTAAGCTAAAGCATGGAAAGAATGATTTATCTCTACTTAGTGTCACGGTTGGCTTGAAAAATTATGATCCACTCTTTGATGAAGTCAATGTTGGGATTCATGGTCCTGTTCAACTGATTGGCAAGTACAAGAATGGTACTGAGGTGACAAAGGACTTGAGCAAAAATGAGTGGATATATAAGGTTGGACTTGCTGGAGAAGAAAAGGGCCTTTACCAAATTACTGGTCATGCTGCTAATTTCCACTGGCCTACAGATAAACTTCCAACAGATAGAATGTTTGTTTGGTACAAGACAATCTTCAAAGCTCCTCTTGGGACCGATCCAGTTGTGGTGGATTTAATGGGTTTAGGAAAGGGTCATGCTTGGGTGAACGGTCGAAGCATTGGCAGGTATTGGACAAGTCACACTGCAGATGAAAATGGTTGTACAGCAAAATGCGACTATCGTGGAACTTACAGCAATAAGAAGTGCTTGACTAATTGTGGGAAACCTTCCCAAAGATGGTACCATATACCGCGATCTTTCCTCCAAGATGACAATAATGCACTAGTTCTCTTTGAGGAGTTTGGAGGCCAGCCGTTCAATGTTGAATTTCAGACTGTAACAGTTGCTAAAGCTTGCGCAAATGCTTACGAAGGTAATGTCTTGCACTTGTCATGCCAAGGAGGAAGAGTTTTATCCGATGTTAGATTTTCCAGCTTCGGTGATCCACAAGGCGCTTGTGGGGCATCCTTCACAAAAGGCGAGTGTGAATCCCCCACAGCTTTATCATACATCCAAAAGGCATGCATTGGCAAAGCAGAATGTTTTATCGATGTCTCTGAAATGACTCTTGGACCAACAGGCTGCAGAGACATGAATCGCCTTGCCGTCGAAGTCGATTGTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gal_Lectin Glyco_hydro_35

Protein Analysis

839

Amino Acids

94.87

Weight (kDa)

6.09

Isoelectric Point (pI)

29.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 36 - 340 4e-122 Glycosyl hydrolases family 35
GHD PF17834 364 - 418 3.3e-21 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 637 - 724 1.6e-22 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 761 - 838 2.3e-13 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2251
Acc36I ACCTGC 2 cut(s) 704, 2007
Acc65I GGTACC 1 cut(s) 2133
AccB1I GGYRCC 2 cut(s) 860, 2133
AccI GTMKAC 1 cut(s) 1236
AccII CGCG 1 cut(s) 2146
AciI CCGC 1 cut(s) 2144
AcoI YGGCCR 1 cut(s) 179
AcsI RAATTY 2 cut(s) 898, 2219
AcuI CTGAAG 2 cut(s) 1251, 1602
AfiI CCNNNNNNNGG 4 cut(s) 295, 992, 993, 2130
AflIII ACRYGT 1 cut(s) 1540
AhlI ACTAGT 1 cut(s) 2176
AjnI CCWGG 3 cut(s) 288, 294, 412
AjuI GAANNNNNNNTTGG 6 cut(s) 1701, 1733, 1894, 1926, 2445, 2477
AluBI AGCT 8 cut(s) 1009, 1083, 1104, 1633, 1934, 2246, 2328, 2393
AluI AGCT 8 cut(s) 1009, 1083, 1104, 1633, 1934, 2246, 2328, 2393
Alw21I GWGCWC 1 cut(s) 1353
Alw26I GTCTC 4 cut(s) 50, 221, 2452, 2475
Ama87I CYCGRG 1 cut(s) 1084
AoxI GGCC 5 cut(s) 179, 211, 1834, 1874, 2200
ApeKI GCWGC 3 cut(s) 400, 1859, 2475
ApoI RAATTY 2 cut(s) 898, 2219
ArsI GACNNNNNNTTYG 6 cut(s) 345, 377, 1837, 1869, 2256, 2288
AseI ATTAAT 1 cut(s) 690
Asp718I GGTACC 1 cut(s) 2133
AspLEI GCGC 3 cut(s) 573, 2252, 2348
AspS9I GGNCC 9 cut(s) 292, 353, 524, 920, 1500, 1730, 1834, 1944, 2465
AsuHPI GGTGA 7 cut(s) 131, 310, 922, 1064, 1783, 2008, 2345
AvaI CYCGRG 1 cut(s) 1084
AvaII GGWCC 8 cut(s) 292, 353, 524, 920, 1500, 1730, 1944, 2465
BaeI ACNNNNGTAYC 2 cut(s) 1755, 1788
BanI GGYRCC 2 cut(s) 860, 2133
BanII GRGCYC 1 cut(s) 333
Bbv12I GWGCWC 1 cut(s) 1353
BbvI GCAGC 3 cut(s) 387, 1846, 2462
BccI CCATC 6 cut(s) 89, 113, 200, 323, 768, 2124
BceAI ACGGC 3 cut(s) 206, 2191, 2483
BciT130I CCWGG 3 cut(s) 290, 296, 414
BclI TGATCA 2 cut(s) 715, 1417
BcoDI GTCTC 4 cut(s) 50, 221, 2452, 2475
BcuI ACTAGT 1 cut(s) 2176
BfaI CTAG 3 cut(s) 42, 741, 2177
BfmI CTRYAG 5 cut(s) 813, 1105, 1878, 2037, 2476
BfoI RGCGCY 1 cut(s) 2349
BfuAI ACCTGC 2 cut(s) 704, 2007
BisI GCNGC 3 cut(s) 401, 1860, 2476
BlpI GCTNAGC 1 cut(s) 1347
BlsI GCNGC 3 cut(s) 402, 1861, 2477
BmcAI AGTACT 1 cut(s) 166
Bme1390I CCNGG 3 cut(s) 290, 296, 414
Bme18I GGWCC 8 cut(s) 292, 353, 524, 920, 1500, 1730, 1944, 2465
BmeT110I CYCGRG 1 cut(s) 1084
BmgT120I GGNCC 9 cut(s) 292, 353, 524, 920, 1500, 1730, 1834, 1944, 2465
BmiI GGNNCC 6 cut(s) 525, 526, 862, 1317, 1945, 2135
BmrFI CCNGG 3 cut(s) 290, 296, 414
BmsI GCATC 7 cut(s) 208, 426, 447, 661, 940, 1006, 2366
BoxI GACNNNNGTC 1 cut(s) 233
BpmI CTGGAG 1 cut(s) 1842
Bpu1102I GCTNAGC 1 cut(s) 1347
BpuEI CTTGAG 2 cut(s) 1349, 1804
Bsa29I ATCGAT 2 cut(s) 1117, 2442
BsaAI YACGTR 1 cut(s) 161
BsaJI CCNNGG 3 cut(s) 647, 886, 2290
BsaXI ACNNNNNCTCC 4 cut(s) 1530, 1560, 2286, 2316
Bsc4I CCNNNNNNNGG 4 cut(s) 295, 992, 993, 2130
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 7 cut(s) 775, 1443, 1467, 1533, 1855, 1877, 1952
Bse3DI GCAATG 2 cut(s) 706, 1159
BseBI CCWGG 3 cut(s) 290, 296, 414
BseCI ATCGAT 2 cut(s) 1117, 2442
BseDI CCNNGG 3 cut(s) 647, 886, 2290
BseGI GGATG 4 cut(s) 315, 779, 2357, 2403
BseLI CCNNNNNNNGG 4 cut(s) 295, 992, 993, 2130
BseMI GCAATG 2 cut(s) 706, 1159
BseMII CTCAG 3 cut(s) 1338, 1511, 1758
BseNI ACTGG 7 cut(s) 775, 1443, 1467, 1533, 1855, 1877, 1952
BseRI GAGGAG 2 cut(s) 1926, 2204
BseXI GCAGC 3 cut(s) 387, 1846, 2462
BsgI GTGCAG 1 cut(s) 387
Bsh1236I CGCG 1 cut(s) 2146
Bsh1285I CGRYCG 2 cut(s) 907, 2005
BshFI GGCC 5 cut(s) 181, 213, 1836, 1876, 2202
BshNI GGYRCC 2 cut(s) 860, 2133
BshVI ATCGAT 2 cut(s) 1117, 2442
BsiEI CGRYCG 2 cut(s) 907, 2005
BsiHKAI GWGCWC 1 cut(s) 1353
BsiHKCI CYCGRG 1 cut(s) 1084
BsiSI CCGG 3 cut(s) 77, 182, 1503
BslFI GGGAC 3 cut(s) 537, 1591, 1957
BslI CCNNNNNNNGG 4 cut(s) 295, 992, 993, 2130
BsmAI GTCTC 4 cut(s) 50, 221, 2452, 2475
BsmFI GGGAC 3 cut(s) 537, 1591, 1957
BsmI GAATGC 2 cut(s) 13, 250
BsnI GGCC 5 cut(s) 181, 213, 1836, 1876, 2202
BsoBI CYCGRG 1 cut(s) 1084
Bsp1286I GDGCHC 2 cut(s) 333, 1353
Bsp1407I TGTACA 1 cut(s) 2054
Bsp1720I GCTNAGC 1 cut(s) 1347
Bsp19I CCATGG 2 cut(s) 647, 886
BspACI CCGC 1 cut(s) 2144
BspANI GGCC 5 cut(s) 181, 213, 1836, 1876, 2202
BspCNI CTCAG 3 cut(s) 1339, 1512, 1759
BspDI ATCGAT 2 cut(s) 1117, 2442
BspFNI CGCG 1 cut(s) 2146
BspHI TCATGA 1 cut(s) 91
BspLI GGNNCC 6 cut(s) 525, 526, 862, 1317, 1945, 2135
BspMAI CTGCAG 3 cut(s) 817, 2041, 2480
BspMI ACCTGC 2 cut(s) 704, 2007
BspQI GCTCTTC 1 cut(s) 94
BspT107I GGYRCC 2 cut(s) 860, 2133
BsrDI GCAATG 2 cut(s) 706, 1159
BsrFI RCCGGY 1 cut(s) 76
BsrGI TGTACA 1 cut(s) 2054
BsrI ACTGG 7 cut(s) 775, 1443, 1467, 1533, 1855, 1877, 1952
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 3 cut(s) 647, 886, 2290
BssT1I CCWWGG 3 cut(s) 647, 886, 2290
Bst2UI CCWGG 3 cut(s) 290, 296, 414
Bst4CI ACNGT 5 cut(s) 54, 1675, 2003, 2230, 2236
Bst6I CTCTTC 2 cut(s) 94, 2293
BstAPI GCANNNNNTGC 1 cut(s) 1282
BstAUI TGTACA 1 cut(s) 2054
BstBAI YACGTR 1 cut(s) 161
BstC8I GCNNGC 4 cut(s) 794, 2204, 2248, 2416
BstDEI CTNAG 5 cut(s) 1347, 1520, 1664, 1767, 2517
BstDSI CCRYGG 2 cut(s) 647, 886
BstF5I GGATG 4 cut(s) 315, 779, 2357, 2403
BstFNI CGCG 1 cut(s) 2146
BstH2I RGCGCY 1 cut(s) 2349
BstHHI GCGC 3 cut(s) 573, 2252, 2348
BstMAI GTCTC 4 cut(s) 50, 221, 2452, 2475
BstMCI CGRYCG 2 cut(s) 907, 2005
BstMWI GCNNNNNNNGC 3 cut(s) 579, 1282, 2354
BstNI CCWGG 3 cut(s) 290, 296, 414
BstNSI RCATGY 3 cut(s) 796, 1544, 2418
BstPAI GACNNNNGTC 1 cut(s) 233
BstSCI CCNGG 3 cut(s) 288, 294, 412
BstSFI CTRYAG 5 cut(s) 813, 1105, 1878, 2037, 2476
BstUI CGCG 1 cut(s) 2146
BstV1I GCAGC 3 cut(s) 387, 1846, 2462
BstX2I RGATCY 2 cut(s) 799, 820
BstXI CCANNNNNNTGG 1 cut(s) 1850
BstYI RGATCY 2 cut(s) 799, 820
Bsu15I ATCGAT 2 cut(s) 1117, 2442
BsuRI GGCC 5 cut(s) 181, 213, 1836, 1876, 2202
BsuTUI ATCGAT 2 cut(s) 1117, 2442
BtgI CCRYGG 2 cut(s) 647, 886
BtgZI GCGATG 1 cut(s) 597
BtsCI GGATG 4 cut(s) 315, 779, 2357, 2403
BtsI GCAGTG 2 cut(s) 1242, 2034
BtsIMutI CAGTG 5 cut(s) 1242, 1450, 1540, 1870, 2034
BveI ACCTGC 2 cut(s) 704, 2007
Cac8I GCNNGC 4 cut(s) 794, 2204, 2248, 2416
CciI TCATGA 1 cut(s) 91
CfoI GCGC 3 cut(s) 573, 2252, 2348
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 9 cut(s) 292, 353, 524, 920, 1500, 1730, 1834, 1944, 2465
ClaI ATCGAT 2 cut(s) 1117, 2442
CseI GACGC 1 cut(s) 1307
CsiI ACCWGGT 1 cut(s) 294
CspCI CAANNNNNGTGG 2 cut(s) 971, 1006
DdeI CTNAG 5 cut(s) 1347, 1520, 1664, 1767, 2517
EaeI YGGCCR 1 cut(s) 179
Eam1104I CTCTTC 2 cut(s) 94, 2293
EarI CTCTTC 2 cut(s) 94, 2293
Eco130I CCWWGG 3 cut(s) 647, 886, 2290
Eco147I AGGCCT 1 cut(s) 213
Eco24I GRGCYC 1 cut(s) 333
Eco32I GATATC 1 cut(s) 442
Eco47I GGWCC 8 cut(s) 292, 353, 524, 920, 1500, 1730, 1944, 2465
Eco57I CTGAAG 2 cut(s) 1251, 1602
Eco88I CYCGRG 1 cut(s) 1084
EcoO109I RGGNCCY 3 cut(s) 524, 920, 1834
EcoRII CCWGG 3 cut(s) 288, 294, 412
EcoRV GATATC 1 cut(s) 442
EcoT14I CCWWGG 3 cut(s) 647, 886, 2290
EcoT22I ATGCAT 2 cut(s) 15, 2420
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 3 cut(s) 647, 886, 2290
FalI AAGNNNNNCTT 6 cut(s) 1910, 1942, 1923, 1955, 2084, 2116
FaqI GGGAC 3 cut(s) 537, 1591, 1957
FauNDI CATATG 2 cut(s) 358, 940
FbaI TGATCA 2 cut(s) 715, 1417
FblI GTMKAC 1 cut(s) 1236
Fnu4HI GCNGC 3 cut(s) 401, 1860, 2476
FokI GGATG 4 cut(s) 302, 786, 2344, 2390
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 3 cut(s) 401, 1860, 2476
FspBI CTAG 3 cut(s) 42, 741, 2177
FspI TGCGCA 1 cut(s) 2251
GlaI GCGC 3 cut(s) 572, 2251, 2347
GluI GCNGC 3 cut(s) 401, 1860, 2476
GsuI CTGGAG 1 cut(s) 1842
HaeII RGCGCY 1 cut(s) 2349
HaeIII GGCC 5 cut(s) 181, 213, 1836, 1876, 2202
HapII CCGG 3 cut(s) 77, 182, 1503
HgaI GACGC 1 cut(s) 1307
HhaI GCGC 3 cut(s) 573, 2252, 2348
Hin6I GCGC 3 cut(s) 571, 2250, 2346
HinP1I GCGC 3 cut(s) 571, 2250, 2346
HincII GTYRAC 3 cut(s) 59, 1069, 1255
HindII GTYRAC 3 cut(s) 59, 1069, 1255
HindIII AAGCTT 1 cut(s) 2244
HpaI GTTAAC 1 cut(s) 1255
HpaII CCGG 3 cut(s) 77, 182, 1503
HphI GGTGA 7 cut(s) 131, 310, 922, 1064, 1783, 2008, 2345
Hpy188I TCNGA 7 cut(s) 312, 348, 1044, 1422, 2226, 2311, 2452
Hpy188III TCNNGA 8 cut(s) 92, 170, 282, 1217, 1310, 1399, 1472, 1590
Hpy99I CGWCG 2 cut(s) 1301, 2504
HpyAV CCTTC 9 cut(s) 581, 738, 874, 1223, 1273, 1618, 2130, 2258, 2371
HpyCH4III ACNGT 5 cut(s) 54, 1675, 2003, 2230, 2236
HpyCH4IV ACGT 3 cut(s) 160, 1056, 1257
HpyF10VI GCNNNNNNNGC 3 cut(s) 579, 1282, 2354
HpyF3I CTNAG 5 cut(s) 1347, 1520, 1664, 1767, 2517
HpySE526I ACGT 3 cut(s) 160, 1056, 1257
HspAI GCGC 3 cut(s) 571, 2250, 2346
KflI GGGWCCC 1 cut(s) 524
KpnI GGTACC 1 cut(s) 2137
Ksp22I TGATCA 2 cut(s) 715, 1417
KspAI GTTAAC 1 cut(s) 1255
LguI GCTCTTC 1 cut(s) 94
LmnI GCTCC 2 cut(s) 679, 1939
Lsp1109I GCAGC 3 cut(s) 387, 1846, 2462
LweI GCATC 7 cut(s) 208, 426, 447, 661, 940, 1006, 2366
MabI ACCWGGT 1 cut(s) 294
MaeI CTAG 3 cut(s) 42, 741, 2177
MaeII ACGT 3 cut(s) 160, 1056, 1257
MaeIII GTNAC 9 cut(s) 1052, 1090, 1133, 1204, 1429, 1669, 1771, 2030, 2230
MboII GAAGA 8 cut(s) 111, 138, 335, 680, 760, 1838, 1918, 2310
MfeI CAATTG 1 cut(s) 1495
MflI RGATCY 2 cut(s) 799, 820
MhlI GDGCHC 2 cut(s) 333, 1353
MlyI GAGTC 1 cut(s) 2452
MmeI TCCRAC 3 cut(s) 1319, 1792, 1916
Mph1103I ATGCAT 2 cut(s) 15, 2420
MseI TTAA 8 cut(s) 690, 723, 854, 1002, 1254, 1560, 1629, 1967
MspI CCGG 3 cut(s) 77, 182, 1503
MspR9I CCNGG 3 cut(s) 290, 296, 414
MunI CAATTG 1 cut(s) 1495
Mva1269I GAATGC 2 cut(s) 13, 250
MvaI CCWGG 3 cut(s) 290, 296, 414
MvnI CGCG 1 cut(s) 2146
MwoI GCNNNNNNNGC 3 cut(s) 579, 1282, 2354
NcoI CCATGG 2 cut(s) 647, 886
NdeI CATATG 2 cut(s) 358, 940
NlaIV GGNNCC 6 cut(s) 525, 526, 862, 1317, 1945, 2135
NmuCI GTSAC 5 cut(s) 1052, 1090, 1669, 1771, 2030
NsbI TGCGCA 1 cut(s) 2251
NsiI ATGCAT 2 cut(s) 15, 2420
NspI RCATGY 3 cut(s) 796, 1544, 2418
PaeI GCATGC 2 cut(s) 796, 2418
PaeR7I CTCGAG 1 cut(s) 1084
PagI TCATGA 1 cut(s) 91
PceI AGGCCT 1 cut(s) 213
PciI ACATGT 1 cut(s) 1540
PciSI GCTCTTC 1 cut(s) 94
PctI GAATGC 2 cut(s) 13, 250
PfeI GAWTC 9 cut(s) 129, 303, 502, 626, 843, 979, 1723, 2381, 2488
PkrI GCNGC 3 cut(s) 402, 1861, 2477
PleI GAGTC 1 cut(s) 2452
PpsI GAGTC 1 cut(s) 2452
Ppu21I YACGTR 1 cut(s) 161
PpuMI RGGWCCY 2 cut(s) 524, 920
PscI ACATGT 1 cut(s) 1540
PshAI GACNNNNGTC 1 cut(s) 233
PshBI ATTAAT 1 cut(s) 690
Psp5II RGGWCCY 2 cut(s) 524, 920
Psp6I CCWGG 3 cut(s) 288, 294, 412
PspGI CCWGG 3 cut(s) 288, 294, 412
PspN4I GGNNCC 6 cut(s) 525, 526, 862, 1317, 1945, 2135
PspPI GGNCC 9 cut(s) 292, 353, 524, 920, 1500, 1730, 1834, 1944, 2465
PspPPI RGGWCCY 2 cut(s) 524, 920
PspXI VCTCGAGB 1 cut(s) 1084
PstI CTGCAG 3 cut(s) 817, 2041, 2480
PsuI RGATCY 2 cut(s) 799, 820
SapI GCTCTTC 1 cut(s) 94
SaqAI TTAA 8 cut(s) 690, 723, 854, 1002, 1254, 1560, 1629, 1967
SatI GCNGC 3 cut(s) 401, 1860, 2476
Sau96I GGNCC 9 cut(s) 292, 353, 524, 920, 1500, 1730, 1834, 1944, 2465
ScaI AGTACT 1 cut(s) 166
SchI GAGTC 1 cut(s) 2452
ScrFI CCNGG 3 cut(s) 290, 296, 414
SduI GDGCHC 2 cut(s) 333, 1353
SexAI ACCWGGT 1 cut(s) 294
SfaNI GCATC 7 cut(s) 208, 426, 447, 661, 940, 1006, 2366
SfcI CTRYAG 5 cut(s) 813, 1105, 1878, 2037, 2476
Sfr274I CTCGAG 1 cut(s) 1084
SinI GGWCC 8 cut(s) 292, 353, 524, 920, 1500, 1730, 1944, 2465
SlaI CTCGAG 1 cut(s) 1084
SmlI CTYRAG 3 cut(s) 1084, 1364, 1783
SmoI CTYRAG 3 cut(s) 1084, 1364, 1783
SpeI ACTAGT 1 cut(s) 2176
SphI GCATGC 2 cut(s) 796, 2418
SseBI AGGCCT 1 cut(s) 213
SsiI CCGC 1 cut(s) 2144
SspMI CTAG 3 cut(s) 42, 741, 2177
StuI AGGCCT 1 cut(s) 213
StyD4I CCNGG 3 cut(s) 288, 294, 412
StyI CCWWGG 3 cut(s) 647, 886, 2290
TaaI ACNGT 5 cut(s) 54, 1675, 2003, 2230, 2236
TaiI ACGT 3 cut(s) 163, 1059, 1260
TaqI TCGA 7 cut(s) 87, 1085, 1117, 2005, 2442, 2502, 2508
TaqII GACCGA 2 cut(s) 893, 1961
TatI WGTACW 4 cut(s) 164, 1098, 1752, 2054
TfiI GAWTC 9 cut(s) 129, 303, 502, 626, 843, 979, 1723, 2381, 2488
Tru1I TTAA 8 cut(s) 690, 723, 854, 1002, 1254, 1560, 1629, 1967
Tru9I TTAA 8 cut(s) 690, 723, 854, 1002, 1254, 1560, 1629, 1967
TscAI CASTG 5 cut(s) 1249, 1450, 1540, 1877, 2041
TseFI GTSAC 5 cut(s) 1052, 1090, 1669, 1771, 2030
TseI GCWGC 3 cut(s) 400, 1859, 2475
Tsp45I GTSAC 5 cut(s) 1052, 1090, 1669, 1771, 2030
TspRI CASTG 5 cut(s) 1249, 1450, 1540, 1877, 2041
VpaK11BI GGWCC 8 cut(s) 292, 353, 524, 920, 1500, 1730, 1944, 2465
VspI ATTAAT 1 cut(s) 690
XapI RAATTY 2 cut(s) 898, 2219
XceI RCATGY 3 cut(s) 796, 1544, 2418
XhoI CTCGAG 1 cut(s) 1084
XmiI GTMKAC 1 cut(s) 1236
XspI CTAG 3 cut(s) 42, 741, 2177
ZrmI AGTACT 1 cut(s) 166
Zsp2I ATGCAT 2 cut(s) 15, 2420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.