Rh5AG211800

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001959
Physical Location & Seq
Forward (+)
25003556 .. 25017671
14116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG211800.1

Sequence Viewer

Length: 2559 bp
ATGGCAACTATGCATTCATATAGATTTTTGTTTCTTGCTTCTAGTCTCTTACTGTGTCAACTATGTTCTGCTTTGCCGGTGGAGGTCGATCATGATGGAAGAGCCATCACAATCAATGGTGATAGAAGAATCATAATCTCTGGCTCTATTCACTACCCACGTAGTAGTCCTGAAATGTGGCCGGACTTGATCCAAAAGGCAAAAGATGGAGGCCTTGATGCCGTTGAGACTTATGTCTTTTGGAATGCACATGAACCTCTGCGTAGGCAGTATGACTTTTCAGGAAACCTGGACCTGGTGAGATTCATTCAGACCATCCAAAAGGCAGGGCTCTATGCTGTTCTTCGGATTGGACCATATGTTTGTGCAGAATGGAATTATGGAGGTTTTCCTGTGTGGCTGCATAACATACCAGGCATCAAATTGAGAACAAACAATGATATCTATAAGAATGAGATGCAAAACTTTACAACTTTGATAGTGGACATGATGCAGCATGAGAATCTTTTTGCTTCACAAGGGGGACCCATTATACTTGCCCAGATTGAAAACGAGTATGGGAATGTTATGCGCTCGTATGGCGATGAAGGAAAGAAATATATACAATGGTGTGCTGATTTAGCTGAATCTTACCAGATTGGTGTACCATGGATTATGTGCCAACAGGAAGATGCTCCACAATCAATGATTAATACCTGCAATGGCTGGTATTGTGATCAATTTAAGCCTTCATACAACACTAGCCCGAAGATGTGGACAGAAAATTGGACTGGATGGTTCAAGGATTGGGGCATGCAAGATCCACACAGAACTGCAGAGGATCTCGCCTTTTCTGTTGCACGATTCTTTCAGTTAAATGGCACCTTCCAAAACTATTATATGTACCATGGAGGAACAAATTTTGGTCGGTCAGCAGGTGGTCCTTACATCACCACATCATACGATTATGATGCACCATTGGATGAATATGGAAATTTGAATCAACCCAAGTGGGGGCATCTTAAAGAGCTTCATAACCTCATTAGATCAATGGAGAAGGTTCTGACTTATGGTTTCGTAAGAGAGGTTGAATACGGAGAGAGTTCGCATGTAACAGTTTACAGTTATGGGGGAAATCGGTCCTGTTTCATTAGTAACGCAAACAGGACCACTGATATAACAATTAATTTTGAGAACTTTATGTATTCTGTTCCTGCTTGGTCTGTGACTATACTTCCTGATTGCCATACCGATGTGTACAACACTGCTCAGGTTAATGTGCAAACATCAATAATGGAGAAGGTGCTAAACGAAGCCGATACCAGGGTAGGGGAACCCTATGAGTTGATTTGGGGGTGGAGGCCTGAGAACCTCAACGAAAATGGCTTAGTTCTGCATAGCAATCTCACTACTAATCAGCTGCTGGATCAAAAAGTAGTCACGAATGATACCAGTGATTACTTATGGTACATCACCAGTCTGGATCACAACGCTATTGATCCCAATTGGACTAGCAAAGAACTTACGCTAAGAGTGAATAGCAGAGGACATATCCTCCATGCTTTTATTAATGGCGAACACATAGGGACTGAAGTTTCTGGACAAGAGTTCAATCCTTTTACGTTAGAGAGAAGAATTGAGCTACAGCATGACAAGAATGATTTATCTCTTCTCAGTGTCACTGTTGGCTTGAAACATTACAATCCATTTTTTGACGAAACAGATGTTGGGATTCATGGTCCTGTTCAACTGATTGGCAAGTACAGGAATGGTACTGAGGTGACAAAGGACTTGAGCAAAAATGAGTGGACATATAAGGTTGGACTTGCTGGTGAACAAAAGGGACTTTATCAAATTACTGGTCATGCTGCTAGTTTTCGCTGGACTACAAATAATCTTCCAACAGATAGGATGTTTGTTTGGTACAAGACAATCTTCAAAGCTCCTCTTGGAACCGATCCGGTTGTGGTCGACTTAAGGGGTCTAGGAAAGGGTCATGCTTGGGTGAATGGCCGAAGCATTGGCAGGTATTGGACAAGTTACACTGCCGATGAAAATGGTTGTACAGAAAAATGCGACTATCGTGGAACTTATAGCAATAGGAAGTGCTTGACTAATTGTGGGAAATCTTCCCAGAGATGGTACCATATTCCGCGATCTTTCCTCCAAGACGATAACAACGCACTAATTCTGTTCGAAGAGTTTGGAGGCAATCCATTTAATGTTAAGTTCCAGACTGTAACTGTTGCTAAAGCTTGCGCAAATGTTTACGAAGGTAAGGTACTGCAGTTGTCATGCCAAGGAGGAAGAGTTCTATCAAATGTTAGGGTTGCCATCTTCGGTGATCCACAAGGCGCTTGCAGTAGATCCTTCAGGAAAGGCCAGTGTGAATCTCCGACAGCTTTATCATACATCCAAAAGGCATGCATTGGCAAAGCGGAATGTTATATCGATGTTTCTGAGACGACTCTTGGACCAACGGGCTGCAAACACATAAATCGTCTTGCTGTTGAAGTCGATTGTTCCCAGAACATCTCAAGTTTGAATGTTACAGACTCCATGTTCTATAAACTGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gal_Lectin Glyco_hydro_35

Protein Analysis

852

Amino Acids

96.89

Weight (kDa)

5.91

Isoelectric Point (pI)

35.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 36 - 339 2e-121 Glycosyl hydrolases family 35
GHD PF17834 364 - 418 1.5e-19 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 633 - 720 6.9e-22 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 757 - 834 1.9e-13 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 905
Acc16I TGCGCA 1 cut(s) 2239
Acc36I ACCTGC 3 cut(s) 704, 905, 1995
Acc65I GGTACC 1 cut(s) 2121
AccB1I GGYRCC 2 cut(s) 860, 2121
AccI GTMKAC 1 cut(s) 1950
AccII CGCG 1 cut(s) 2134
AciI CCGC 2 cut(s) 2132, 2417
AclWI GGATC 9 cut(s) 184, 794, 828, 1413, 1470, 1472, 1931, 2318, 2340
AcoI YGGCCR 2 cut(s) 179, 1990
AcsI RAATTY 2 cut(s) 898, 973
AcuI CTGAAG 2 cut(s) 1590, 2335
AfiI CCNNNNNNNGG 6 cut(s) 295, 992, 993, 1302, 1308, 2118
AflII CTTAAG 1 cut(s) 1954
AjnI CCWGG 4 cut(s) 288, 294, 412, 1301
AjuI GAANNNNNNNTTGG 2 cut(s) 1689, 1721
AloI GAACNNNNNNTCC 2 cut(s) 2274, 2306
AluBI AGCT 7 cut(s) 623, 1009, 1399, 1621, 1922, 2234, 2381
AluI AGCT 7 cut(s) 623, 1009, 1399, 1621, 1922, 2234, 2381
Alw26I GTCTC 3 cut(s) 50, 221, 2435
AlwI GGATC 9 cut(s) 184, 794, 828, 1413, 1470, 1472, 1931, 2318, 2340
AlwNI CAGNNNCTG 1 cut(s) 1402
AoxI GGCC 5 cut(s) 179, 211, 1340, 1990, 2359
ApeKI GCWGC 5 cut(s) 400, 493, 1399, 1847, 2463
ApoI RAATTY 2 cut(s) 898, 973
ArsI GACNNNNNNTTYG 4 cut(s) 345, 377, 1825, 1857
AseI ATTAAT 3 cut(s) 690, 1164, 1548
Asp718I GGTACC 1 cut(s) 2121
AspLEI GCGC 3 cut(s) 573, 2240, 2336
AspS9I GGNCC 8 cut(s) 292, 353, 524, 920, 1119, 1146, 1718, 2453
AsuHPI GGTGA 8 cut(s) 131, 310, 922, 1444, 1771, 1823, 1996, 2333
AsuII TTCGAA 1 cut(s) 2175
AvaII GGWCC 8 cut(s) 292, 353, 524, 920, 1119, 1146, 1718, 2453
BaeI ACNNNNGTAYC 6 cut(s) 1290, 1323, 1743, 1776, 2252, 2285
BanI GGYRCC 2 cut(s) 860, 2121
BanII GRGCYC 1 cut(s) 333
BarI GAAGNNNNNNTAC 2 cut(s) 2244, 2276
BbvI GCAGC 5 cut(s) 387, 505, 1386, 1834, 2450
BccI CCATC 7 cut(s) 89, 113, 200, 323, 768, 2112, 2321
BceAI ACGGC 1 cut(s) 206
BcgI CGANNNNNNTGC 2 cut(s) 932, 966
BciT130I CCWGG 4 cut(s) 290, 296, 414, 1303
BclI TGATCA 1 cut(s) 715
BcoDI GTCTC 3 cut(s) 50, 221, 2435
BfaI CTAG 5 cut(s) 42, 741, 1491, 1851, 1964
BfmI CTRYAG 3 cut(s) 813, 1622, 2264
BfoI RGCGCY 1 cut(s) 2337
BfrI CTTAAG 1 cut(s) 1954
BfuAI ACCTGC 3 cut(s) 704, 905, 1995
BisI GCNGC 5 cut(s) 401, 494, 1400, 1848, 2464
BlsI GCNGC 5 cut(s) 402, 495, 1401, 1849, 2465
Bme1390I CCNGG 4 cut(s) 290, 296, 414, 1303
Bme18I GGWCC 8 cut(s) 292, 353, 524, 920, 1119, 1146, 1718, 2453
BmgT120I GGNCC 8 cut(s) 292, 353, 524, 920, 1119, 1146, 1718, 2453
BmiI GGNNCC 6 cut(s) 525, 526, 862, 1314, 1933, 2123
BmrFI CCNGG 4 cut(s) 290, 296, 414, 1303
BmsI GCATC 7 cut(s) 208, 426, 447, 480, 661, 940, 1006
BoxI GACNNNNGTC 1 cut(s) 233
Bpu10I CCTNAGC 1 cut(s) 1248
Bpu14I TTCGAA 1 cut(s) 2175
BpuEI CTTGAG 2 cut(s) 1792, 2500
Bsa29I ATCGAT 1 cut(s) 2430
BsaAI YACGTR 1 cut(s) 161
BsaJI CCNNGG 4 cut(s) 647, 886, 1302, 2278
BsaWI WCCGGW 1 cut(s) 1939
BsaXI ACNNNNNCTCC 4 cut(s) 1518, 1548, 2274, 2304
Bsc4I CCNNNNNNNGG 6 cut(s) 295, 992, 993, 1302, 1308, 2118
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 5 cut(s) 775, 1431, 1455, 1843, 2362
Bse3DI GCAATG 1 cut(s) 706
BseBI CCWGG 4 cut(s) 290, 296, 414, 1303
BseCI ATCGAT 1 cut(s) 2430
BseDI CCNNGG 4 cut(s) 647, 886, 1302, 2278
BseGI GGATG 5 cut(s) 315, 779, 967, 1896, 2391
BseLI CCNNNNNNNGG 6 cut(s) 295, 992, 993, 1302, 1308, 2118
BseMI GCAATG 1 cut(s) 706
BseMII CTCAG 5 cut(s) 1262, 1335, 1666, 1746, 2430
BseNI ACTGG 5 cut(s) 775, 1431, 1455, 1843, 2362
BseRI GAGGAG 1 cut(s) 1914
BseXI GCAGC 5 cut(s) 387, 505, 1386, 1834, 2450
BsgI GTGCAG 1 cut(s) 387
Bsh1236I CGCG 1 cut(s) 2134
BshFI GGCC 5 cut(s) 181, 213, 1342, 1992, 2361
BshNI GGYRCC 2 cut(s) 860, 2121
BshVI ATCGAT 1 cut(s) 2430
BsiSI CCGG 3 cut(s) 77, 182, 1940
BslFI GGGAC 3 cut(s) 537, 1579, 1836
BslI CCNNNNNNNGG 6 cut(s) 295, 992, 993, 1302, 1308, 2118
BsmAI GTCTC 3 cut(s) 50, 221, 2435
BsmBI CGTCTC 1 cut(s) 2435
BsmFI GGGAC 3 cut(s) 537, 1579, 1836
BsmI GAATGC 2 cut(s) 13, 250
BsnI GGCC 5 cut(s) 181, 213, 1342, 1992, 2361
Bsp119I TTCGAA 1 cut(s) 2175
Bsp1286I GDGCHC 1 cut(s) 333
Bsp1407I TGTACA 2 cut(s) 1236, 2042
Bsp19I CCATGG 2 cut(s) 647, 886
BspACI CCGC 2 cut(s) 2132, 2417
BspANI GGCC 5 cut(s) 181, 213, 1342, 1992, 2361
BspCNI CTCAG 5 cut(s) 1261, 1336, 1665, 1747, 2431
BspDI ATCGAT 1 cut(s) 2430
BspFNI CGCG 1 cut(s) 2134
BspHI TCATGA 1 cut(s) 91
BspLI GGNNCC 6 cut(s) 525, 526, 862, 1314, 1933, 2123
BspMAI CTGCAG 2 cut(s) 817, 2268
BspMI ACCTGC 3 cut(s) 704, 905, 1995
BspPI GGATC 9 cut(s) 184, 794, 828, 1413, 1470, 1472, 1931, 2318, 2340
BspQI GCTCTTC 1 cut(s) 94
BspT104I TTCGAA 1 cut(s) 2175
BspT107I GGYRCC 2 cut(s) 860, 2121
BspTI CTTAAG 1 cut(s) 1954
BsrDI GCAATG 1 cut(s) 706
BsrFI RCCGGY 1 cut(s) 76
BsrGI TGTACA 2 cut(s) 1236, 2042
BsrI ACTGG 5 cut(s) 775, 1431, 1455, 1843, 2362
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 4 cut(s) 647, 886, 1302, 2278
BssT1I CCWWGG 3 cut(s) 647, 886, 2278
Bst2UI CCWGG 4 cut(s) 290, 296, 414, 1303
Bst4CI ACNGT 6 cut(s) 54, 1096, 1103, 1663, 2218, 2224
Bst6I CTCTTC 4 cut(s) 94, 1653, 2172, 2281
BstAFI CTTAAG 1 cut(s) 1954
BstAUI TGTACA 2 cut(s) 1236, 2042
BstBAI YACGTR 1 cut(s) 161
BstBI TTCGAA 1 cut(s) 2175
BstC8I GCNNGC 4 cut(s) 794, 2236, 2338, 2404
BstDEI CTNAG 7 cut(s) 1248, 1344, 1366, 1508, 1652, 1755, 2439
BstDSI CCRYGG 2 cut(s) 647, 886
BstF5I GGATG 5 cut(s) 315, 779, 967, 1896, 2391
BstFNI CGCG 1 cut(s) 2134
BstH2I RGCGCY 1 cut(s) 2337
BstHHI GCGC 3 cut(s) 573, 2240, 2336
BstMAI GTCTC 3 cut(s) 50, 221, 2435
BstMWI GCNNNNNNNGC 2 cut(s) 579, 620
BstNI CCWGG 4 cut(s) 290, 296, 414, 1303
BstNSI RCATGY 3 cut(s) 796, 1091, 2406
BstPAI GACNNNNGTC 1 cut(s) 233
BstSCI CCNGG 4 cut(s) 288, 294, 412, 1301
BstSFI CTRYAG 3 cut(s) 813, 1622, 2264
BstUI CGCG 1 cut(s) 2134
BstV1I GCAGC 5 cut(s) 387, 505, 1386, 1834, 2450
BstX2I RGATCY 3 cut(s) 799, 820, 2345
BstYI RGATCY 3 cut(s) 799, 820, 2345
Bsu15I ATCGAT 1 cut(s) 2430
BsuRI GGCC 5 cut(s) 181, 213, 1342, 1992, 2361
BsuTUI ATCGAT 1 cut(s) 2430
BtgI CCRYGG 2 cut(s) 647, 886
BtgZI GCGATG 1 cut(s) 597
BtsCI GGATG 5 cut(s) 315, 779, 967, 1896, 2391
BtsI GCAGTG 2 cut(s) 1242, 2022
BtsIMutI CAGTG 7 cut(s) 1149, 1242, 1438, 1659, 1660, 2022, 2369
BveI ACCTGC 3 cut(s) 704, 905, 1995
Cac8I GCNNGC 4 cut(s) 794, 2236, 2338, 2404
CaiI CAGNNNCTG 1 cut(s) 1402
CciI TCATGA 1 cut(s) 91
CfoI GCGC 3 cut(s) 573, 2240, 2336
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 8 cut(s) 292, 353, 524, 920, 1119, 1146, 1718, 2453
ClaI ATCGAT 1 cut(s) 2430
CsiI ACCWGGT 1 cut(s) 294
CspCI CAANNNNNGTGG 2 cut(s) 971, 1006
DdeI CTNAG 7 cut(s) 1248, 1344, 1366, 1508, 1652, 1755, 2439
EaeI YGGCCR 2 cut(s) 179, 1990
Eam1104I CTCTTC 4 cut(s) 94, 1653, 2172, 2281
EarI CTCTTC 4 cut(s) 94, 1653, 2172, 2281
Eco130I CCWWGG 3 cut(s) 647, 886, 2278
Eco147I AGGCCT 2 cut(s) 213, 1342
Eco24I GRGCYC 1 cut(s) 333
Eco32I GATATC 1 cut(s) 442
Eco47I GGWCC 8 cut(s) 292, 353, 524, 920, 1119, 1146, 1718, 2453
Eco57I CTGAAG 2 cut(s) 1590, 2335
EcoO109I RGGNCCY 1 cut(s) 524
EcoRII CCWGG 4 cut(s) 288, 294, 412, 1301
EcoRV GATATC 1 cut(s) 442
EcoT14I CCWWGG 3 cut(s) 647, 886, 2278
EcoT22I ATGCAT 2 cut(s) 15, 2408
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 3 cut(s) 647, 886, 2278
Esp3I CGTCTC 1 cut(s) 2435
FalI AAGNNNNNCTT 4 cut(s) 1898, 1930, 1911, 1943
FaqI GGGAC 3 cut(s) 537, 1579, 1836
FauNDI CATATG 1 cut(s) 358
FbaI TGATCA 1 cut(s) 715
FblI GTMKAC 1 cut(s) 1950
Fnu4HI GCNGC 5 cut(s) 401, 494, 1400, 1848, 2464
FokI GGATG 5 cut(s) 302, 786, 974, 1903, 2378
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 5 cut(s) 401, 494, 1400, 1848, 2464
FspBI CTAG 5 cut(s) 42, 741, 1491, 1851, 1964
FspI TGCGCA 1 cut(s) 2239
GlaI GCGC 3 cut(s) 572, 2239, 2335
GluI GCNGC 5 cut(s) 401, 494, 1400, 1848, 2464
HaeII RGCGCY 1 cut(s) 2337
HaeIII GGCC 5 cut(s) 181, 213, 1342, 1992, 2361
HapII CCGG 3 cut(s) 77, 182, 1940
HhaI GCGC 3 cut(s) 573, 2240, 2336
Hin6I GCGC 3 cut(s) 571, 2238, 2334
HinP1I GCGC 3 cut(s) 571, 2238, 2334
HincII GTYRAC 2 cut(s) 59, 1951
HindII GTYRAC 2 cut(s) 59, 1951
HindIII AAGCTT 1 cut(s) 2232
HpaII CCGG 3 cut(s) 77, 182, 1940
HphI GGTGA 8 cut(s) 131, 310, 922, 1444, 1771, 1823, 1996, 2333
Hpy188I TCNGA 5 cut(s) 312, 348, 1044, 2376, 2440
Hpy188III TCNNGA 9 cut(s) 92, 170, 282, 1217, 1420, 1460, 1578, 2212, 2353
HpyAV CCTTC 7 cut(s) 581, 738, 874, 1030, 1273, 2246, 2359
HpyCH4III ACNGT 6 cut(s) 54, 1096, 1103, 1663, 2218, 2224
HpyCH4IV ACGT 2 cut(s) 160, 1601
HpyF10VI GCNNNNNNNGC 2 cut(s) 579, 620
HpyF3I CTNAG 7 cut(s) 1248, 1344, 1366, 1508, 1652, 1755, 2439
HpySE526I ACGT 2 cut(s) 160, 1601
HspAI GCGC 3 cut(s) 571, 2238, 2334
KflI GGGWCCC 1 cut(s) 524
KpnI GGTACC 1 cut(s) 2125
Ksp22I TGATCA 1 cut(s) 715
LguI GCTCTTC 1 cut(s) 94
LmnI GCTCC 2 cut(s) 679, 1927
Lsp1109I GCAGC 5 cut(s) 387, 505, 1386, 1834, 2450
LweI GCATC 7 cut(s) 208, 426, 447, 480, 661, 940, 1006
MabI ACCWGGT 1 cut(s) 294
MaeI CTAG 5 cut(s) 42, 741, 1491, 1851, 1964
MaeII ACGT 2 cut(s) 160, 1601
MaeIII GTNAC 9 cut(s) 1090, 1133, 1204, 1417, 1657, 1759, 2018, 2218, 2527
MfeI CAATTG 1 cut(s) 1483
MflI RGATCY 3 cut(s) 799, 820, 2345
MhlI GDGCHC 1 cut(s) 333
MlyI GAGTC 2 cut(s) 2440, 2528
MmeI TCCRAC 3 cut(s) 1780, 1904, 2399
Mph1103I ATGCAT 2 cut(s) 15, 2408
MslI CAYNNNNRTG 1 cut(s) 1230
MspA1I CMGCKG 1 cut(s) 1399
MspCI CTTAAG 1 cut(s) 1954
MspI CCGG 3 cut(s) 77, 182, 1940
MspR9I CCNGG 4 cut(s) 290, 296, 414, 1303
MunI CAATTG 1 cut(s) 1483
Mva1269I GAATGC 2 cut(s) 13, 250
MvaI CCWGG 4 cut(s) 290, 296, 414, 1303
MvnI CGCG 1 cut(s) 2134
MwoI GCNNNNNNNGC 2 cut(s) 579, 620
NcoI CCATGG 2 cut(s) 647, 886
NdeI CATATG 1 cut(s) 358
NlaIV GGNNCC 6 cut(s) 525, 526, 862, 1314, 1933, 2123
NmuCI GTSAC 4 cut(s) 1204, 1417, 1657, 1759
NsbI TGCGCA 1 cut(s) 2239
NsiI ATGCAT 2 cut(s) 15, 2408
NspI RCATGY 3 cut(s) 796, 1091, 2406
NspV TTCGAA 1 cut(s) 2175
PaeI GCATGC 2 cut(s) 796, 2406
PagI TCATGA 1 cut(s) 91
PaqCI CACCTGC 1 cut(s) 905
PceI AGGCCT 2 cut(s) 213, 1342
PciSI GCTCTTC 1 cut(s) 94
PctI GAATGC 2 cut(s) 13, 250
PfeI GAWTC 8 cut(s) 129, 303, 502, 626, 843, 979, 1711, 2369
PkrI GCNGC 5 cut(s) 402, 495, 1401, 1849, 2465
PleI GAGTC 2 cut(s) 2440, 2528
PpsI GAGTC 2 cut(s) 2440, 2528
Ppu21I YACGTR 1 cut(s) 161
PpuMI RGGWCCY 1 cut(s) 524
PshAI GACNNNNGTC 1 cut(s) 233
PshBI ATTAAT 3 cut(s) 690, 1164, 1548
Psp5II RGGWCCY 1 cut(s) 524
Psp6I CCWGG 4 cut(s) 288, 294, 412, 1301
PspGI CCWGG 4 cut(s) 288, 294, 412, 1301
PspN4I GGNNCC 6 cut(s) 525, 526, 862, 1314, 1933, 2123
PspPI GGNCC 8 cut(s) 292, 353, 524, 920, 1119, 1146, 1718, 2453
PspPPI RGGWCCY 1 cut(s) 524
PstI CTGCAG 2 cut(s) 817, 2268
PstNI CAGNNNCTG 1 cut(s) 1402
PsuI RGATCY 3 cut(s) 799, 820, 2345
PvuII CAGCTG 1 cut(s) 1399
RseI CAYNNNNRTG 1 cut(s) 1230
SalI GTCGAC 1 cut(s) 1949
SapI GCTCTTC 1 cut(s) 94
SatI GCNGC 5 cut(s) 401, 494, 1400, 1848, 2464
Sau96I GGNCC 8 cut(s) 292, 353, 524, 920, 1119, 1146, 1718, 2453
SchI GAGTC 2 cut(s) 2440, 2528
ScrFI CCNGG 4 cut(s) 290, 296, 414, 1303
SduI GDGCHC 1 cut(s) 333
SexAI ACCWGGT 1 cut(s) 294
SfaNI GCATC 7 cut(s) 208, 426, 447, 480, 661, 940, 1006
SfcI CTRYAG 3 cut(s) 813, 1622, 2264
SfuI TTCGAA 1 cut(s) 2175
SinI GGWCC 8 cut(s) 292, 353, 524, 920, 1119, 1146, 1718, 2453
SmiMI CAYNNNNRTG 1 cut(s) 1230
SmlI CTYRAG 3 cut(s) 1771, 1954, 2515
SmoI CTYRAG 3 cut(s) 1771, 1954, 2515
SphI GCATGC 2 cut(s) 796, 2406
SseBI AGGCCT 2 cut(s) 213, 1342
SsiI CCGC 2 cut(s) 2132, 2417
SspMI CTAG 5 cut(s) 42, 741, 1491, 1851, 1964
StuI AGGCCT 2 cut(s) 213, 1342
StyD4I CCNGG 4 cut(s) 288, 294, 412, 1301
StyI CCWWGG 3 cut(s) 647, 886, 2278
TaaI ACNGT 6 cut(s) 54, 1096, 1103, 1663, 2218, 2224
TaiI ACGT 2 cut(s) 163, 1604
TaqI TCGA 5 cut(s) 87, 1950, 2175, 2430, 2496
TaqII GACCGA 2 cut(s) 897, 1107
TatI WGTACW 3 cut(s) 1236, 1740, 2042
TfiI GAWTC 8 cut(s) 129, 303, 502, 626, 843, 979, 1711, 2369
TscAI CASTG 7 cut(s) 1156, 1249, 1438, 1660, 1666, 2029, 2369
TseFI GTSAC 4 cut(s) 1204, 1417, 1657, 1759
TseI GCWGC 5 cut(s) 400, 493, 1399, 1847, 2463
Tsp45I GTSAC 4 cut(s) 1204, 1417, 1657, 1759
TspGWI ACGGA 1 cut(s) 1089
TspRI CASTG 7 cut(s) 1156, 1249, 1438, 1660, 1666, 2029, 2369
Vha464I CTTAAG 1 cut(s) 1954
VpaK11BI GGWCC 8 cut(s) 292, 353, 524, 920, 1119, 1146, 1718, 2453
VspI ATTAAT 3 cut(s) 690, 1164, 1548
XapI RAATTY 2 cut(s) 898, 973
XceI RCATGY 3 cut(s) 796, 1091, 2406
XmiI GTMKAC 1 cut(s) 1950
XspI CTAG 5 cut(s) 42, 741, 1491, 1851, 1964
Zsp2I ATGCAT 2 cut(s) 15, 2408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.