Rorug05G0120200

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001959
Physical Location & Seq
Forward (+)
10593062 .. 10595391
2330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0120200.1

Sequence Viewer

Length: 609 bp
ATGGCGAACGAGAAGCCACTGAGGAAAATTGCAGAGGCATTCAAGGAGCTGGAGGCCGCCGTGAAATCGGAGACCGGCGAAGTGGAGGTGGCGCGGTTCTCGCAGGCTTGCTCTCTCGTCTCGCCGCTGTTCGGGTGCTTAGGGATCGCTTTCAAGTTCGCCGAGATCGACTACGTCGCAAAGGTGCATGATCTGGCGGAGGCGTCGAAATCAATCTCGACCCTACACGTGTTGGTGGACCGGGATGTGGAGGCGGGCACTGTGAGAAAGGCGGGTGGTCATGCGAGGAATTTGCTGAGGGTGAAGCGTGGGATTGATATGGTGAAGGTGCTGTTTGAGCAGATTATAGTCACCAAGGGAAATTCCTTGAAGGATCCAGCTTCCAAGGCATATCAACAGGTGTTTGCACCCCATCATGGGTGGGTCATCAGGAAAGCTGTTGCTGCCGGGATGTATGCGCTTCCTACAAAAGAACAACTATTGCACAAACTCAATGAAGATGAGACCTCGGCAAAAGCTCAAATGCAAAATTATGTTGCCGCTTCAGCACCTCTAATTCTGTACATTGACAAACTCTTCCAGACAAGGAATTTGGGTGTGGATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gal_Lectin Glyco_hydro_35

Protein Analysis

202

Amino Acids

22.28

Weight (kDa)

8.98

Isoelectric Point (pI)

23.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GLTP PF08718 28 - 166 5.1e-40 Glycolipid transfer protein (GLTP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 94
AciI CCGC 7 cut(s) 57, 94, 125, 197, 254, 272, 540
AclWI GGATC 3 cut(s) 152, 368, 381
AcsI RAATTY 3 cut(s) 289, 361, 589
AcuI CTGAAG 1 cut(s) 528
AcvI CACGTG 1 cut(s) 229
AcyI GRCGYC 1 cut(s) 203
AfaI GTAC 1 cut(s) 563
AfiI CCNNNNNNNGG 4 cut(s) 131, 247, 416, 417
AflIII ACRYGT 2 cut(s) 226, 228
AgsI TTSAA 3 cut(s) 43, 154, 370
AluBI AGCT 4 cut(s) 49, 380, 437, 518
AluI AGCT 4 cut(s) 49, 380, 437, 518
Alw26I GTCTC 3 cut(s) 65, 124, 497
AlwI GGATC 3 cut(s) 152, 368, 381
AoxI GGCC 1 cut(s) 54
ApeKI GCWGC 1 cut(s) 443
ApoI RAATTY 3 cut(s) 289, 361, 589
ArsI GACNNNNNNTTYG 2 cut(s) 574, 606
AspLEI GCGC 2 cut(s) 94, 460
AspS9I GGNCC 1 cut(s) 238
AsuC2I CCSGG 2 cut(s) 242, 448
AsuHPI GGTGA 3 cut(s) 313, 334, 343
AvaII GGWCC 1 cut(s) 238
BaeGI GKGCMC 1 cut(s) 260
BamHI GGATCC 1 cut(s) 373
BbrPI CACGTG 1 cut(s) 229
BbvCI CCTCAGC 1 cut(s) 296
BbvI GCAGC 1 cut(s) 430
BccI CCATC 1 cut(s) 420
BceAI ACGGC 1 cut(s) 44
BcgI CGANNNNNNTGC 2 cut(s) 274, 308
BcnI CCSGG 2 cut(s) 242, 448
BcoDI GTCTC 3 cut(s) 65, 124, 497
BisI GCNGC 4 cut(s) 57, 125, 444, 540
BlsI GCNGC 4 cut(s) 58, 126, 445, 541
Bme1390I CCNGG 2 cut(s) 242, 448
Bme18I GGWCC 1 cut(s) 238
BmgT120I GGNCC 1 cut(s) 238
BmiI GGNNCC 1 cut(s) 375
BmrFI CCNGG 2 cut(s) 242, 448
BpmI CTGGAG 1 cut(s) 71
Bpu10I CCTNAGC 2 cut(s) 139, 296
BpuMI CCSGG 2 cut(s) 242, 448
BsaAI YACGTR 1 cut(s) 229
BsaHI GRCGYC 1 cut(s) 203
BsaI GGTCTC 2 cut(s) 65, 497
BsaJI CCNNGG 3 cut(s) 354, 384, 507
Bsc4I CCNNNNNNNGG 4 cut(s) 131, 247, 416, 417
Bse118I RCCGGY 1 cut(s) 74
BseDI CCNNGG 3 cut(s) 354, 384, 507
BseGI GGATG 2 cut(s) 250, 456
BseLI CCNNNNNNNGG 4 cut(s) 131, 247, 416, 417
BseMII CTCAG 2 cut(s) 11, 287
BseSI GKGCMC 1 cut(s) 260
BseXI GCAGC 1 cut(s) 430
Bsh1236I CGCG 1 cut(s) 94
BshFI GGCC 1 cut(s) 56
BsiSI CCGG 3 cut(s) 75, 241, 447
BslI CCNNNNNNNGG 4 cut(s) 131, 247, 416, 417
BsmAI GTCTC 3 cut(s) 65, 124, 497
BsmBI CGTCTC 1 cut(s) 124
BsmI GAATGC 1 cut(s) 38
BsnI GGCC 1 cut(s) 56
Bso31I GGTCTC 2 cut(s) 65, 497
Bsp1286I GDGCHC 1 cut(s) 260
Bsp1407I TGTACA 1 cut(s) 561
Bsp143I GATC 4 cut(s) 144, 165, 190, 373
BspACI CCGC 7 cut(s) 57, 94, 125, 197, 254, 272, 540
BspANI GGCC 1 cut(s) 56
BspCNI CTCAG 2 cut(s) 12, 288
BspFNI CGCG 1 cut(s) 94
BspLI GGNNCC 1 cut(s) 375
BspPI GGATC 3 cut(s) 152, 368, 381
BspTNI GGTCTC 2 cut(s) 65, 497
BsrFI RCCGGY 1 cut(s) 74
BsrGI TGTACA 1 cut(s) 561
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 3 cut(s) 354, 384, 507
BssMI GATC 4 cut(s) 144, 165, 190, 373
BssNI GRCGYC 1 cut(s) 203
BssT1I CCWWGG 2 cut(s) 354, 384
Bst4CI ACNGT 1 cut(s) 262
Bst6I CTCTTC 1 cut(s) 581
BstACI GRCGYC 1 cut(s) 203
BstAUI TGTACA 1 cut(s) 561
BstBAI YACGTR 1 cut(s) 229
BstC8I GCNNGC 3 cut(s) 105, 109, 256
BstDEI CTNAG 3 cut(s) 20, 139, 296
BstF5I GGATG 2 cut(s) 250, 456
BstFNI CGCG 1 cut(s) 94
BstHHI GCGC 2 cut(s) 94, 460
BstKTI GATC 4 cut(s) 147, 168, 193, 376
BstMAI GTCTC 3 cut(s) 65, 124, 497
BstMBI GATC 4 cut(s) 144, 165, 190, 373
BstMWI GCNNNNNNNGC 5 cut(s) 100, 337, 386, 443, 545
BstSCI CCNGG 2 cut(s) 240, 446
BstSLI GKGCMC 1 cut(s) 260
BstUI CGCG 1 cut(s) 94
BstV1I GCAGC 1 cut(s) 430
BstX2I RGATCY 1 cut(s) 373
BstYI RGATCY 1 cut(s) 373
BsuRI GGCC 1 cut(s) 56
BtsCI GGATG 2 cut(s) 250, 456
BtsIMutI CAGTG 2 cut(s) 17, 258
Cac8I GCNNGC 3 cut(s) 105, 109, 256
CfoI GCGC 2 cut(s) 94, 460
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 238
CseI GACGC 1 cut(s) 192
Csp6I GTAC 1 cut(s) 562
CviAII CATG 3 cut(s) 188, 281, 416
CviJI RGCY 7 cut(s) 16, 49, 56, 107, 380, 437, 518
CviKI_1 RGCY 7 cut(s) 16, 49, 56, 107, 380, 437, 518
CviQI GTAC 1 cut(s) 562
DdeI CTNAG 3 cut(s) 20, 139, 296
DpnI GATC 4 cut(s) 146, 167, 192, 375
DpnII GATC 4 cut(s) 144, 165, 190, 373
Eam1104I CTCTTC 1 cut(s) 581
EarI CTCTTC 1 cut(s) 581
EciI GGCGGA 1 cut(s) 212
Eco130I CCWWGG 2 cut(s) 354, 384
Eco31I GGTCTC 2 cut(s) 65, 497
Eco47I GGWCC 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 528
Eco72I CACGTG 1 cut(s) 229
EcoT14I CCWWGG 2 cut(s) 354, 384
ErhI CCWWGG 2 cut(s) 354, 384
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 3 cut(s) 191, 284, 419
FaiI YATR 8 cut(s) 189, 282, 320, 347, 391, 417, 456, 534
FatI CATG 3 cut(s) 187, 280, 415
FauI CCCGC 2 cut(s) 247, 265
Fnu4HI GCNGC 4 cut(s) 57, 125, 444, 540
FokI GGATG 2 cut(s) 257, 463
Fsp4HI GCNGC 4 cut(s) 57, 125, 444, 540
GlaI GCGC 2 cut(s) 93, 459
GluI GCNGC 4 cut(s) 57, 125, 444, 540
GsuI CTGGAG 1 cut(s) 71
HaeIII GGCC 1 cut(s) 56
HapII CCGG 3 cut(s) 75, 241, 447
HgaI GACGC 1 cut(s) 192
HhaI GCGC 2 cut(s) 94, 460
Hin1I GRCGYC 1 cut(s) 203
Hin1II CATG 3 cut(s) 191, 284, 419
Hin6I GCGC 2 cut(s) 92, 458
HinP1I GCGC 2 cut(s) 92, 458
HpaII CCGG 3 cut(s) 75, 241, 447
HphI GGTGA 3 cut(s) 313, 334, 343
Hpy166II GTNNAC 1 cut(s) 238
Hpy188I TCNGA 1 cut(s) 70
Hpy188III TCNNGA 3 cut(s) 217, 430, 580
Hpy8I GTNNAC 1 cut(s) 238
Hpy99I CGWCG 2 cut(s) 179, 208
HpyAV CCTTC 2 cut(s) 319, 364
HpyCH4III ACNGT 1 cut(s) 262
HpyCH4IV ACGT 2 cut(s) 174, 228
HpyCH4V TGCA 5 cut(s) 32, 187, 407, 484, 526
HpyF10VI GCNNNNNNNGC 5 cut(s) 100, 337, 386, 443, 545
HpyF3I CTNAG 3 cut(s) 20, 139, 296
HpySE526I ACGT 2 cut(s) 174, 228
Hsp92I GRCGYC 1 cut(s) 203
Hsp92II CATG 3 cut(s) 191, 284, 419
HspAI GCGC 2 cut(s) 92, 458
Kzo9I GATC 4 cut(s) 144, 165, 190, 373
LmnI GCTCC 1 cut(s) 46
Lsp1109I GCAGC 1 cut(s) 430
MaeII ACGT 2 cut(s) 174, 228
MaeIII GTNAC 1 cut(s) 349
MalI GATC 4 cut(s) 146, 167, 192, 375
MboI GATC 4 cut(s) 144, 165, 190, 373
MboII GAAGA 2 cut(s) 509, 568
MflI RGATCY 1 cut(s) 373
MhlI GDGCHC 1 cut(s) 260
MluCI AATT 6 cut(s) 27, 289, 361, 529, 555, 589
MspA1I CMGCKG 1 cut(s) 127
MspI CCGG 3 cut(s) 75, 241, 447
MspR9I CCNGG 2 cut(s) 242, 448
Mva1269I GAATGC 1 cut(s) 38
MvnI CGCG 1 cut(s) 94
MwoI GCNNNNNNNGC 5 cut(s) 100, 337, 386, 443, 545
NciI CCSGG 2 cut(s) 242, 448
NdeII GATC 4 cut(s) 144, 165, 190, 373
NlaIII CATG 3 cut(s) 191, 284, 419
NlaIV GGNNCC 1 cut(s) 375
NmeAIII GCCGAG 2 cut(s) 187, 488
NmuCI GTSAC 1 cut(s) 349
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PctI GAATGC 1 cut(s) 38
PflFI GACNNNGTC 1 cut(s) 173
PkrI GCNGC 4 cut(s) 58, 126, 445, 541
PmaCI CACGTG 1 cut(s) 229
PmlI CACGTG 1 cut(s) 229
Ppu21I YACGTR 1 cut(s) 229
PspCI CACGTG 1 cut(s) 229
PspN4I GGNNCC 1 cut(s) 375
PspPI GGNCC 1 cut(s) 238
PsuI RGATCY 1 cut(s) 373
PsyI GACNNNGTC 1 cut(s) 173
RsaI GTAC 1 cut(s) 563
RsaNI GTAC 1 cut(s) 562
SatI GCNGC 4 cut(s) 57, 125, 444, 540
Sau3AI GATC 4 cut(s) 144, 165, 190, 373
Sau96I GGNCC 1 cut(s) 238
ScrFI CCNGG 2 cut(s) 242, 448
SduI GDGCHC 1 cut(s) 260
SinI GGWCC 1 cut(s) 238
Sse9I AATT 6 cut(s) 27, 289, 361, 529, 555, 589
SsiI CCGC 7 cut(s) 57, 94, 125, 197, 254, 272, 540
StyD4I CCNGG 2 cut(s) 240, 446
StyI CCWWGG 2 cut(s) 354, 384
TaaI ACNGT 1 cut(s) 262
TaiI ACGT 2 cut(s) 177, 231
TaqI TCGA 3 cut(s) 168, 206, 218
TasI AATT 6 cut(s) 27, 289, 361, 529, 555, 589
TatI WGTACW 1 cut(s) 561
TauI GCSGC 3 cut(s) 59, 127, 542
TscAI CASTG 2 cut(s) 24, 265
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 1 cut(s) 443
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 1 cut(s) 510
TspRI CASTG 2 cut(s) 24, 265
Tth111I GACNNNGTC 1 cut(s) 173
VpaK11BI GGWCC 1 cut(s) 238
XapI RAATTY 3 cut(s) 289, 361, 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.