Rw5G019420

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001959
Physical Location & Seq
Forward (+)
25280779 .. 25285234
4456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G019420.1

Sequence Viewer

Length: 2460 bp
ATGGCAACTATGCATTCATATAGATTTTTGTTTCTTGCTTCTAGTCTCTTACTGTGTCAACTATGTTCTGCTTTGCCGGTGGAGGTCGATCATGATGGAAGAGCCATCACAATCAATGGTGATAGAAGAATCATAATCTCTGGCTCTATTCACTACCCACGTAGTACTCCTGAAATGTGGCCGGACTTGATCCAAAATGCAAAAGATGGAGGCCTTGATGCCGTTGAGACTTATGTCTTTTGGAATGCACATGAACCTCTGCGTAGGCAGTATGACTTTTCAGGAAACCTGGACCTGGTGAGATTCATTCAGACCATCCAAAAGGCAGGGCTCTATGCTGTTCTTCGGATTGGACCATATGTTTGTGCAGAATGGAATTATGGTTTTCCTGTGTGGCTGCATAACATACCAGGCATCAAATTGAGAACAAACAATGATATCTATAAGAATGAGATGCAAAACTTTACAACTTTGATAGTGGACATGATGAAGCATGAGAATCTTTTTGCTTCACAAGGGGGACCCATTATACTTGCCCAGATTGAAAACGAGTATGGGAATGTTATGCGCTCGTATGGCGATGAAGGAAAGAAATATATACAATGGTGTGCTGATTTAGCTGAATCTTACCAGATTGGTGTACCATGGATTATGTGCCAACAGGAAGATGCTCCACAATCAATGATTAATACCTGCAATGGCTGGTATTGTGATCAATTTAAGCCTTCATACAACACTAGCCCGAAGATGTGGACAGAAAATTGGACTGGATGGTTCAAGGATTGGGGCATGCAAGATCCACACAGAACTGCAGAGGATCTCGCCTTTTCTGTTGCACGATTCTTTCAGTTAAATGGCACCTTCCAAAACTATTATATGTACCATGGAGGAACAAATTTCGGTCGTTCAGCAGGAGGTCCTTACATCACCACATCATATGATTATGATGCACCATTAGATGAATATGGTAATTTGAATCAACCCAAGTGGGGGCATCTTAAAGAGCTTCATAAACATATAAGATCAATGGAGAAAGTTCTGACTTATGGTGACGTAAGACAGGTTGACTATGGAAACAGCTCGAGTGTGACAGAGTACAGCTACAGAGGAAATCGATCCTGTTTCATTAGTAACGCAAACACAACAAGCAATGTAATAGTGAACTTTGAGAATAATAAGTATTCTGTTCCTGCTTGGTCTGTTAATGTGCAAAGATCAATAATGCAGAAGGTGCTAAATGAAGCCGATGCTAACGATCCAGAGGAACCTTATGGTTTAGTTTGGGGTTGGAGGGCTGAGCACTTTACACACCTCAAGGAAAATGGATCAGTTCTCCATAGCAATCTCACGACTAATCAACTCCTTGATCAGAAAGTGGTAACAAATGATACCAGTGATTACTTGTGGTACATTACCAGTCTGGATCACAATGCAACTGATCCCAATTGGTCCGGCAAAGAAATTACGCTGAGAGTGAATACCAGTGGACATGTCCTCCATGCTTTTGTTAATGGAAAACACATAGTTTCTGGACAACATTTCAATCCCTTCACTTTAGAGAGAAAGATTAAGCTAAAGCATGGAAAGAATGATTTATCTCTACTTAGTGTCACGGTTGGCTTGAAAAATTATGATCCACTCTTTGATGAAGTCAATGTTGGGATTCATGGTCCTGTTCAACTGATTGGCAAGTACAAGAATAGTACTGAGGTGACAAAGGACTTGAGCAAAAATGAGTGGATATATAAGGTTGGACTTGCTGGAGAAGAAAAGGGCCTTTACCAAATTACTGGTCATGCTGCTAATTTCCACTGGCCTACAGATAAACTTCCAACAGATAGAATGTTTGTTTGGTACAAGACAATCTTCAAAGCTCCTCTTGGGACCGATCCAGTTGTGGTGGATTTAATGGGTTTAGGAAAGGGTCATGCTTGGGTGAACGGTCGAAGCATTGGCAGGTATTGGACAAGTCACACTGCAGATGAAAATGGTTGTACAGCAAAATGCGACTATCGTGGAACTTACAGCAATAAGAAGTGCTTGACTAATTGTGGGAAACCTTCCCAAAGATGGTACCATATACCGCGATCTTTCCTCCAAGATGACAATAATGCACTAGTTCTATTTGAGGAGTTTGGAGGCCAGCCGTTCAATGTTGAATTTCAGACTGTAACAGTTGCTAAAGCTTGCGCAAATGCTTACGAAGGTAATGTCTTGCACTTGTCATGCCAAGGAGGAAGAGTTTTATCCGATGTTAGATTTTCCAGCTTCGGTGATCCACAAGGCGCTTGTGGGGCATCCTTCACAAAAGGCGAGTGTGAATCCCCCACAGCTTTATCATACATCCAAAAGGCATGCATTGGCAAAGCAGAATGTTTTATCGATGTCTCTGAAATGACTCTTGGACCAACAGGCTGCAGAGACATGAATCGCCTTGCCGTCGAAGTCGATTGTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gal_Lectin Glyco_hydro_35

Protein Analysis

819

Amino Acids

92.71

Weight (kDa)

6.14

Isoelectric Point (pI)

29.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 36 - 339 1.6e-118 Glycosyl hydrolases family 35
GHD PF17834 363 - 403 7.1e-10 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 617 - 704 1.6e-22 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 741 - 818 2.3e-13 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2191
Acc36I ACCTGC 2 cut(s) 701, 1947
Acc65I GGTACC 1 cut(s) 2073
AccB1I GGYRCC 2 cut(s) 857, 2073
AccII CGCG 1 cut(s) 2086
AciI CCGC 1 cut(s) 2084
AcoI YGGCCR 1 cut(s) 179
AcsI RAATTY 2 cut(s) 895, 2159
AfiI CCNNNNNNNGG 4 cut(s) 295, 989, 990, 2070
AflIII ACRYGT 1 cut(s) 1489
AgsI TTSAA 9 cut(s) 545, 778, 976, 1543, 1624, 1679, 1870, 2152, 2159
AhlI ACTAGT 1 cut(s) 2116
AjnI CCWGG 3 cut(s) 288, 294, 409
AjuI GAANNNNNNNTTGG 6 cut(s) 1641, 1673, 1834, 1866, 2385, 2417
AluBI AGCT 9 cut(s) 620, 1006, 1080, 1101, 1573, 1874, 2186, 2268, 2333
AluI AGCT 9 cut(s) 620, 1006, 1080, 1101, 1573, 1874, 2186, 2268, 2333
Alw21I GWGCWC 1 cut(s) 1302
Alw26I GTCTC 4 cut(s) 50, 221, 2392, 2415
Ama87I CYCGRG 1 cut(s) 1081
AoxI GGCC 5 cut(s) 179, 211, 1774, 1814, 2140
ApeKI GCWGC 3 cut(s) 397, 1799, 2415
ApoI RAATTY 2 cut(s) 895, 2159
ArsI GACNNNNNNTTYG 6 cut(s) 345, 377, 1777, 1809, 2196, 2228
AseI ATTAAT 1 cut(s) 687
Asp718I GGTACC 1 cut(s) 2073
AspLEI GCGC 3 cut(s) 570, 2192, 2288
AspS9I GGNCC 9 cut(s) 292, 353, 521, 917, 1449, 1670, 1774, 1884, 2405
AsuHPI GGTGA 7 cut(s) 131, 310, 919, 1061, 1723, 1948, 2285
AvaI CYCGRG 1 cut(s) 1081
AvaII GGWCC 8 cut(s) 292, 353, 521, 917, 1449, 1670, 1884, 2405
BanI GGYRCC 2 cut(s) 857, 2073
BanII GRGCYC 1 cut(s) 333
Bbv12I GWGCWC 1 cut(s) 1302
BbvI GCAGC 3 cut(s) 384, 1786, 2402
BccI CCATC 6 cut(s) 89, 113, 200, 323, 765, 2064
BceAI ACGGC 3 cut(s) 206, 2131, 2423
BciT130I CCWGG 3 cut(s) 290, 296, 411
BclI TGATCA 2 cut(s) 712, 1366
BcoDI GTCTC 4 cut(s) 50, 221, 2392, 2415
BcuI ACTAGT 1 cut(s) 2116
BfaI CTAG 3 cut(s) 42, 738, 2117
BfmI CTRYAG 5 cut(s) 810, 1102, 1818, 1977, 2416
BfoI RGCGCY 1 cut(s) 2289
BfuAI ACCTGC 2 cut(s) 701, 1947
BisI GCNGC 3 cut(s) 398, 1800, 2416
BlpI GCTNAGC 1 cut(s) 1296
BlsI GCNGC 3 cut(s) 399, 1801, 2417
BmcAI AGTACT 2 cut(s) 166, 1705
Bme1390I CCNGG 3 cut(s) 290, 296, 411
Bme18I GGWCC 8 cut(s) 292, 353, 521, 917, 1449, 1670, 1884, 2405
BmeT110I CYCGRG 1 cut(s) 1081
BmgT120I GGNCC 9 cut(s) 292, 353, 521, 917, 1449, 1670, 1774, 1884, 2405
BmiI GGNNCC 6 cut(s) 522, 523, 859, 1266, 1885, 2075
BmrFI CCNGG 3 cut(s) 290, 296, 411
BmsI GCATC 8 cut(s) 208, 423, 444, 658, 937, 1003, 1237, 2306
BoxI GACNNNNGTC 1 cut(s) 233
BpmI CTGGAG 1 cut(s) 1782
Bpu1102I GCTNAGC 1 cut(s) 1296
BpuEI CTTGAG 2 cut(s) 1298, 1744
Bsa29I ATCGAT 2 cut(s) 1114, 2382
BsaAI YACGTR 1 cut(s) 161
BsaJI CCNNGG 3 cut(s) 644, 883, 2230
BsaXI ACNNNNNCTCC 4 cut(s) 1479, 1509, 2226, 2256
Bsc4I CCNNNNNNNGG 4 cut(s) 295, 989, 990, 2070
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 7 cut(s) 772, 1392, 1416, 1482, 1795, 1817, 1892
Bse3DI GCAATG 2 cut(s) 703, 1156
BseBI CCWGG 3 cut(s) 290, 296, 411
BseCI ATCGAT 2 cut(s) 1114, 2382
BseDI CCNNGG 3 cut(s) 644, 883, 2230
BseGI GGATG 4 cut(s) 315, 776, 2297, 2343
BseLI CCNNNNNNNGG 4 cut(s) 295, 989, 990, 2070
BseMI GCAATG 2 cut(s) 703, 1156
BseMII CTCAG 3 cut(s) 1287, 1460, 1698
BseNI ACTGG 7 cut(s) 772, 1392, 1416, 1482, 1795, 1817, 1892
BseRI GAGGAG 2 cut(s) 1866, 2144
BseXI GCAGC 3 cut(s) 384, 1786, 2402
BsgI GTGCAG 1 cut(s) 387
Bsh1236I CGCG 1 cut(s) 2086
Bsh1285I CGRYCG 2 cut(s) 904, 1945
BshFI GGCC 5 cut(s) 181, 213, 1776, 1816, 2142
BshNI GGYRCC 2 cut(s) 857, 2073
BshVI ATCGAT 2 cut(s) 1114, 2382
BsiEI CGRYCG 2 cut(s) 904, 1945
BsiHKAI GWGCWC 1 cut(s) 1302
BsiHKCI CYCGRG 1 cut(s) 1081
BsiSI CCGG 3 cut(s) 77, 182, 1452
BslFI GGGAC 2 cut(s) 534, 1897
BslI CCNNNNNNNGG 4 cut(s) 295, 989, 990, 2070
BsmAI GTCTC 4 cut(s) 50, 221, 2392, 2415
BsmFI GGGAC 2 cut(s) 534, 1897
BsmI GAATGC 2 cut(s) 13, 250
BsnI GGCC 5 cut(s) 181, 213, 1776, 1816, 2142
BsoBI CYCGRG 1 cut(s) 1081
Bsp1286I GDGCHC 2 cut(s) 333, 1302
Bsp1407I TGTACA 1 cut(s) 1994
Bsp1720I GCTNAGC 1 cut(s) 1296
Bsp19I CCATGG 2 cut(s) 644, 883
BspACI CCGC 1 cut(s) 2084
BspANI GGCC 5 cut(s) 181, 213, 1776, 1816, 2142
BspCNI CTCAG 3 cut(s) 1288, 1461, 1699
BspDI ATCGAT 2 cut(s) 1114, 2382
BspFNI CGCG 1 cut(s) 2086
BspHI TCATGA 1 cut(s) 91
BspLI GGNNCC 6 cut(s) 522, 523, 859, 1266, 1885, 2075
BspMAI CTGCAG 3 cut(s) 814, 1981, 2420
BspMI ACCTGC 2 cut(s) 701, 1947
BspQI GCTCTTC 1 cut(s) 94
BspT107I GGYRCC 2 cut(s) 857, 2073
BsrDI GCAATG 2 cut(s) 703, 1156
BsrFI RCCGGY 1 cut(s) 76
BsrGI TGTACA 1 cut(s) 1994
BsrI ACTGG 7 cut(s) 772, 1392, 1416, 1482, 1795, 1817, 1892
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 3 cut(s) 644, 883, 2230
BssT1I CCWWGG 3 cut(s) 644, 883, 2230
Bst2UI CCWGG 3 cut(s) 290, 296, 411
Bst4CI ACNGT 5 cut(s) 54, 1615, 1943, 2170, 2176
Bst6I CTCTTC 2 cut(s) 94, 2233
BstAPI GCANNNNNTGC 1 cut(s) 1231
BstAUI TGTACA 1 cut(s) 1994
BstBAI YACGTR 1 cut(s) 161
BstC8I GCNNGC 4 cut(s) 791, 2144, 2188, 2356
BstDEI CTNAG 5 cut(s) 1296, 1469, 1604, 1707, 2457
BstDSI CCRYGG 2 cut(s) 644, 883
BstF5I GGATG 4 cut(s) 315, 776, 2297, 2343
BstFNI CGCG 1 cut(s) 2086
BstH2I RGCGCY 1 cut(s) 2289
BstHHI GCGC 3 cut(s) 570, 2192, 2288
BstMAI GTCTC 4 cut(s) 50, 221, 2392, 2415
BstMCI CGRYCG 2 cut(s) 904, 1945
BstMWI GCNNNNNNNGC 4 cut(s) 576, 617, 1231, 2294
BstNI CCWGG 3 cut(s) 290, 296, 411
BstNSI RCATGY 3 cut(s) 793, 1493, 2358
BstPAI GACNNNNGTC 1 cut(s) 233
BstSCI CCNGG 3 cut(s) 288, 294, 409
BstSFI CTRYAG 5 cut(s) 810, 1102, 1818, 1977, 2416
BstUI CGCG 1 cut(s) 2086
BstV1I GCAGC 3 cut(s) 384, 1786, 2402
BstX2I RGATCY 2 cut(s) 796, 817
BstXI CCANNNNNNTGG 1 cut(s) 1790
BstYI RGATCY 2 cut(s) 796, 817
Bsu15I ATCGAT 2 cut(s) 1114, 2382
BsuRI GGCC 5 cut(s) 181, 213, 1776, 1816, 2142
BsuTUI ATCGAT 2 cut(s) 1114, 2382
BtgI CCRYGG 2 cut(s) 644, 883
BtgZI GCGATG 1 cut(s) 594
BtsCI GGATG 4 cut(s) 315, 776, 2297, 2343
BtsI GCAGTG 1 cut(s) 1974
BtsIMutI CAGTG 4 cut(s) 1399, 1489, 1810, 1974
BveI ACCTGC 2 cut(s) 701, 1947
Cac8I GCNNGC 4 cut(s) 791, 2144, 2188, 2356
CciI TCATGA 1 cut(s) 91
CfoI GCGC 3 cut(s) 570, 2192, 2288
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 9 cut(s) 292, 353, 521, 917, 1449, 1670, 1774, 1884, 2405
ClaI ATCGAT 2 cut(s) 1114, 2382
CsiI ACCWGGT 1 cut(s) 294
CspCI CAANNNNNGTGG 2 cut(s) 968, 1003
DdeI CTNAG 5 cut(s) 1296, 1469, 1604, 1707, 2457
EaeI YGGCCR 1 cut(s) 179
Eam1104I CTCTTC 2 cut(s) 94, 2233
EarI CTCTTC 2 cut(s) 94, 2233
Eco130I CCWWGG 3 cut(s) 644, 883, 2230
Eco147I AGGCCT 1 cut(s) 213
Eco24I GRGCYC 1 cut(s) 333
Eco32I GATATC 1 cut(s) 439
Eco47I GGWCC 8 cut(s) 292, 353, 521, 917, 1449, 1670, 1884, 2405
Eco88I CYCGRG 1 cut(s) 1081
EcoO109I RGGNCCY 3 cut(s) 521, 917, 1774
EcoRII CCWGG 3 cut(s) 288, 294, 409
EcoRV GATATC 1 cut(s) 439
EcoT14I CCWWGG 3 cut(s) 644, 883, 2230
EcoT22I ATGCAT 2 cut(s) 15, 2360
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 3 cut(s) 644, 883, 2230
FalI AAGNNNNNCTT 6 cut(s) 1850, 1882, 1863, 1895, 2024, 2056
FaqI GGGAC 2 cut(s) 534, 1897
FauNDI CATATG 2 cut(s) 358, 937
FbaI TGATCA 2 cut(s) 712, 1366
Fnu4HI GCNGC 3 cut(s) 398, 1800, 2416
FokI GGATG 4 cut(s) 302, 783, 2284, 2330
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 3 cut(s) 398, 1800, 2416
FspBI CTAG 3 cut(s) 42, 738, 2117
FspI TGCGCA 1 cut(s) 2191
GlaI GCGC 3 cut(s) 569, 2191, 2287
GluI GCNGC 3 cut(s) 398, 1800, 2416
GsuI CTGGAG 1 cut(s) 1782
HaeII RGCGCY 1 cut(s) 2289
HaeIII GGCC 5 cut(s) 181, 213, 1776, 1816, 2142
HapII CCGG 3 cut(s) 77, 182, 1452
HhaI GCGC 3 cut(s) 570, 2192, 2288
Hin6I GCGC 3 cut(s) 568, 2190, 2286
HinP1I GCGC 3 cut(s) 568, 2190, 2286
HincII GTYRAC 2 cut(s) 59, 1066
HindII GTYRAC 2 cut(s) 59, 1066
HindIII AAGCTT 1 cut(s) 2184
HpaII CCGG 3 cut(s) 77, 182, 1452
HphI GGTGA 7 cut(s) 131, 310, 919, 1061, 1723, 1948, 2285
Hpy166II GTNNAC 8 cut(s) 59, 481, 641, 753, 1066, 1162, 1487, 1939
Hpy188I TCNGA 7 cut(s) 312, 348, 1041, 1371, 2166, 2251, 2392
Hpy188III TCNNGA 7 cut(s) 92, 170, 282, 1259, 1348, 1421, 1530
Hpy8I GTNNAC 8 cut(s) 59, 481, 641, 753, 1066, 1162, 1487, 1939
Hpy99I CGWCG 1 cut(s) 2444
HpyAV CCTTC 8 cut(s) 578, 735, 871, 1222, 1558, 2070, 2198, 2311
HpyCH4III ACNGT 5 cut(s) 54, 1615, 1943, 2170, 2176
HpyCH4IV ACGT 2 cut(s) 160, 1053
HpyF10VI GCNNNNNNNGC 4 cut(s) 576, 617, 1231, 2294
HpyF3I CTNAG 5 cut(s) 1296, 1469, 1604, 1707, 2457
HpySE526I ACGT 2 cut(s) 160, 1053
HspAI GCGC 3 cut(s) 568, 2190, 2286
KflI GGGWCCC 1 cut(s) 521
KpnI GGTACC 1 cut(s) 2077
Ksp22I TGATCA 2 cut(s) 712, 1366
LguI GCTCTTC 1 cut(s) 94
LmnI GCTCC 2 cut(s) 676, 1879
Lsp1109I GCAGC 3 cut(s) 384, 1786, 2402
LweI GCATC 8 cut(s) 208, 423, 444, 658, 937, 1003, 1237, 2306
MabI ACCWGGT 1 cut(s) 294
MaeI CTAG 3 cut(s) 42, 738, 2117
MaeII ACGT 2 cut(s) 160, 1053
MaeIII GTNAC 8 cut(s) 1049, 1087, 1130, 1378, 1609, 1711, 1970, 2170
MboII GAAGA 8 cut(s) 111, 138, 335, 677, 757, 1778, 1858, 2250
MfeI CAATTG 1 cut(s) 1444
MflI RGATCY 2 cut(s) 796, 817
MhlI GDGCHC 2 cut(s) 333, 1302
MlyI GAGTC 1 cut(s) 2392
MmeI TCCRAC 3 cut(s) 1268, 1732, 1856
Mph1103I ATGCAT 2 cut(s) 15, 2360
MseI TTAA 8 cut(s) 687, 720, 851, 999, 1203, 1509, 1569, 1907
MspI CCGG 3 cut(s) 77, 182, 1452
MspR9I CCNGG 3 cut(s) 290, 296, 411
MunI CAATTG 1 cut(s) 1444
Mva1269I GAATGC 2 cut(s) 13, 250
MvaI CCWGG 3 cut(s) 290, 296, 411
MvnI CGCG 1 cut(s) 2086
MwoI GCNNNNNNNGC 4 cut(s) 576, 617, 1231, 2294
NcoI CCATGG 2 cut(s) 644, 883
NdeI CATATG 2 cut(s) 358, 937
NlaIV GGNNCC 6 cut(s) 522, 523, 859, 1266, 1885, 2075
NmuCI GTSAC 5 cut(s) 1049, 1087, 1609, 1711, 1970
NsbI TGCGCA 1 cut(s) 2191
NsiI ATGCAT 2 cut(s) 15, 2360
NspI RCATGY 3 cut(s) 793, 1493, 2358
PaeI GCATGC 2 cut(s) 793, 2358
PaeR7I CTCGAG 1 cut(s) 1081
PagI TCATGA 1 cut(s) 91
PceI AGGCCT 1 cut(s) 213
PciI ACATGT 1 cut(s) 1489
PciSI GCTCTTC 1 cut(s) 94
PctI GAATGC 2 cut(s) 13, 250
PfeI GAWTC 9 cut(s) 129, 303, 499, 623, 840, 976, 1663, 2321, 2428
PkrI GCNGC 3 cut(s) 399, 1801, 2417
PleI GAGTC 1 cut(s) 2392
PpsI GAGTC 1 cut(s) 2392
Ppu21I YACGTR 1 cut(s) 161
PpuMI RGGWCCY 2 cut(s) 521, 917
PscI ACATGT 1 cut(s) 1489
PshAI GACNNNNGTC 1 cut(s) 233
PshBI ATTAAT 1 cut(s) 687
Psp5II RGGWCCY 2 cut(s) 521, 917
Psp6I CCWGG 3 cut(s) 288, 294, 409
PspGI CCWGG 3 cut(s) 288, 294, 409
PspN4I GGNNCC 6 cut(s) 522, 523, 859, 1266, 1885, 2075
PspPI GGNCC 9 cut(s) 292, 353, 521, 917, 1449, 1670, 1774, 1884, 2405
PspPPI RGGWCCY 2 cut(s) 521, 917
PspXI VCTCGAGB 1 cut(s) 1081
PstI CTGCAG 3 cut(s) 814, 1981, 2420
PsuI RGATCY 2 cut(s) 796, 817
SapI GCTCTTC 1 cut(s) 94
SaqAI TTAA 8 cut(s) 687, 720, 851, 999, 1203, 1509, 1569, 1907
SatI GCNGC 3 cut(s) 398, 1800, 2416
Sau96I GGNCC 9 cut(s) 292, 353, 521, 917, 1449, 1670, 1774, 1884, 2405
ScaI AGTACT 2 cut(s) 166, 1705
SchI GAGTC 1 cut(s) 2392
ScrFI CCNGG 3 cut(s) 290, 296, 411
SduI GDGCHC 2 cut(s) 333, 1302
SexAI ACCWGGT 1 cut(s) 294
SfaNI GCATC 8 cut(s) 208, 423, 444, 658, 937, 1003, 1237, 2306
SfcI CTRYAG 5 cut(s) 810, 1102, 1818, 1977, 2416
Sfr274I CTCGAG 1 cut(s) 1081
SinI GGWCC 8 cut(s) 292, 353, 521, 917, 1449, 1670, 1884, 2405
SlaI CTCGAG 1 cut(s) 1081
SmlI CTYRAG 3 cut(s) 1081, 1313, 1723
SmoI CTYRAG 3 cut(s) 1081, 1313, 1723
SpeI ACTAGT 1 cut(s) 2116
SphI GCATGC 2 cut(s) 793, 2358
SseBI AGGCCT 1 cut(s) 213
SsiI CCGC 1 cut(s) 2084
SspMI CTAG 3 cut(s) 42, 738, 2117
StuI AGGCCT 1 cut(s) 213
StyD4I CCNGG 3 cut(s) 288, 294, 409
StyI CCWWGG 3 cut(s) 644, 883, 2230
TaaI ACNGT 5 cut(s) 54, 1615, 1943, 2170, 2176
TaiI ACGT 2 cut(s) 163, 1056
TaqI TCGA 7 cut(s) 87, 1082, 1114, 1945, 2382, 2442, 2448
TaqII GACCGA 2 cut(s) 890, 1901
TatI WGTACW 5 cut(s) 164, 1095, 1692, 1703, 1994
TfiI GAWTC 9 cut(s) 129, 303, 499, 623, 840, 976, 1663, 2321, 2428
Tru1I TTAA 8 cut(s) 687, 720, 851, 999, 1203, 1509, 1569, 1907
Tru9I TTAA 8 cut(s) 687, 720, 851, 999, 1203, 1509, 1569, 1907
TscAI CASTG 4 cut(s) 1399, 1489, 1817, 1981
TseFI GTSAC 5 cut(s) 1049, 1087, 1609, 1711, 1970
TseI GCWGC 3 cut(s) 397, 1799, 2415
Tsp45I GTSAC 5 cut(s) 1049, 1087, 1609, 1711, 1970
TspRI CASTG 4 cut(s) 1399, 1489, 1817, 1981
VpaK11BI GGWCC 8 cut(s) 292, 353, 521, 917, 1449, 1670, 1884, 2405
VspI ATTAAT 1 cut(s) 687
XapI RAATTY 2 cut(s) 895, 2159
XceI RCATGY 3 cut(s) 793, 1493, 2358
XhoI CTCGAG 1 cut(s) 1081
XspI CTAG 3 cut(s) 42, 738, 2117
ZrmI AGTACT 2 cut(s) 166, 1705
Zsp2I ATGCAT 2 cut(s) 15, 2360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.