Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0001959

Physical Location & Seq

Genomic Coordinates Forward (+)

7369316 .. 7373166

Gene Structure 3851 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G007570.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2445 bp

ATGGCACTCCCCAAGAGTTTTGCGTTATCTCTTTGTTTCTGTCTTTCTGTGATCCTCTGCAGCCTCGTTGCTAATGCTACTGACGTTTCTTATGATGGCAGAGCCATCACCATTGATGGCAAACGAAGGATTTTGAATTCTGGTTCCATACATTATCCTCGAAGTACACCACAAATGTGGCCAGACTTGATTAGGAAGTCAAAGGAAGGTGGACTCGATGCAATTGAGACTTACGTCTTCTGGAATGCGCATGAACCAGTTCGTCGTGAATACGATTTTACTGGTAATCTTGACCTGGTTAGGTTTATCAAAACGATTCAAGATGAAGGACTTTATGCTGTTCTTCGGATTGGTCCATATGTTTGTGCTGAATGGAATTATGGAGGACTTCCTGTTTGGCTCCATAACCTGCCAAACTGTCAAATTCGAACAGCAAACGATGTTTTCATGAATGAGATGAAGAATTTCACTACATTGATCGTGGATATGATGAAAAAAGAGAAACTATTTGCATCTCAAGGAGGCCCTATCATTATTGCTCAGATTGAAAATGAATATGGGAATGTGGAATCATACTATGGAGATGCTGGAAAAGCATACATTAACTGGTCTGCAAACTTTGCTGAATCACTAAACATCGGAGTTCCATGGATTATGTGTCAACAAAAAGACGCTCCCGCATCAATGATAAACACATGTAATGGCTGGTATTGTGACACATTCAAGCCAAGTAACCCCAACACTCCTAAGATGTGGACTGAAAATTGGACTGGCTGGTTCAAGAGCTGGGGTGGTTTAGACCCACTCAGGACTGCTGAGGACGTTGCCTTTGCAGTAGCAAGGTTTTTCCAATATGGAGGCACATTTCAAAACTATTACATGTATCATGGTGGAACAAACTTTGGTAGAACAGCAGCTTCATACATTACCACCTCGTATGATTATGATGCCCCTCTTGATGAGTATGAGCTTCATGAGGTTTTGAAATCCATGGAATATACTCTCACCTATGGCAATATTACCACCACAGAATTGGGTAACTCTGTCTCAACCACTGTGTTTGCGACAAATGAGTTAAGTAGCTGCATTTTTGGCAATGCAAATAGCAGTTCAGATGTTACAATCACTTTCCAAGGAAACACATACACCATTCCTGCTTGGTCTGTTAGTATTCTTCCTGATTGCAAAAAAGAAGCATACAACACTGCTAAGGTAAACACCCAAACTACAGTGAAAGTAAAAAGTGTCAACAAGGCTGAGGATGAACCTGAATCCCTTCATTGGTCATGGAGGCCCGAGAAGACGGATGACACCCAGCTTCTTGGAAAAGGTGAAGTTGTGGCAAGTCATCTTATGGAACAAAAAGAGGCTGCAAATGATGCTAGTGATTATCTTTGGTACATGACAAGTGTTCAACTCAGTAAGGATGATCCAATTTTGAGTGGGAACATGACATTGAGGATAAATGAAACTGGTTACATTCTTCATGCTTATGTTAATGGAGAACATGTTGGTTCTCAATGGGCTGCTTATAACGTCTTCAACTACGTTTTCGAGAAACCTATCAAGTTGGTTCCCGGAGTGAACACAATTTCATTGCTCAGTGCTACTGTTGGATTTCCGAATTATGGAGGTGGATTTGAGGACATCAAAACTGGAGTAGCTGGTCCTGTTCAATTGGTTGGACAAAGTGGAGATGAAACTGTGATCAAGGATTTGTCAGAACACAAATGGTCATACAAGGTTGGACTCCATGGCTTGAAAGACGAGCTCTTTAGCTCTGCTTCTCGTTTTGCAAATAAATGGTCAGTTGAAGGTTTACCAGTAAACAGAATGATGACATGGTATAAGACAACTTTCAAAGCTCCCCTAGGAACTGAACCAGTTGTCGTGGACTTACACGGGTTGGGTAAGGGATATGCTTGGGTGAACGGGCATAGCATTGGGCGATATTGGCCAAGCTACTTGGCTCCAGAGGATGGTTGTAGTGTTGAAGCTTGTGACTACCGTGGTGCATACGACAATAACAAGTGTGTCTTCAACTGTGGCAAACCTACTCAAAGATGGTATCATGTTCCGCGATCCTTTTTGGATGATGAGAACACATTGGTCTTGTTTGAGGAATTTGGTGGCAACCCATCTTATGTGAATTTCCAGACAATCAGAGTTGGAACTATTTGTGCAAATGCATATGAGAATCATACATTGGAATTGGCATGCCAAGGTCGCCCCATTTCAGCAATCAAATTTGCTAGCTTTGGAAAACCTGAAGGGACTTGTGGATCATTTAAGAATGGCACCTGTGATGGCACAACAGCTTTGTCCATCCTTCAAAAGGAATGTGTTGGAAAAGAGTCGTGCTCAATTGATGTATCAGAAAAGAAGTTCGGTTCCACAAATTGTGGCGATATTGTGAAGAGGCTGGCCGTGGAAGCAGTTTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BetaGal_dom4_5 Gal_Lectin Glyco_hydro_35

Protein Analysis

814

Amino Acids

90.62

Weight (kDa)

5.38

Isoelectric Point (pI)

30.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	36 - 324	8.5e-113	Glycosyl hydrolases family 35
Glyco_hydro_42	PF02449	57 - 206	8.2e-07	Beta-galactosidase
GHD	PF17834	351 - 405	7.2e-21	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	613 - 699	2.6e-23	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	739 - 814	3.8e-18	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001959)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31740 AT2G04060 AT5G20710 AT5G20710
fragaria_vesca	FvH4_1g09120 FvH4_1g09190 FvH4_1g09190 FvH4_1g26380
malus_domestica	MD02G1096400.v1.1 MD03G1276000.v1.1 MD15G1220900.v1.1 MD15G1221000.v1.1
prunus_persica	Prupe.7G194500_v2.0.a1
pyrus_communis	pycom02g07640
rosa_chinensis	RchiOBHm_Chr2g0095511 RchiOBHm_Chr2g0095591 RchiOBHm_Chr5g0030701
rosa_laevigata	RLG00000016574 RLG00000016582 RLG00000016583
rosa_multiflora	Rmu_co8462209.1_g000001 Rmu_sc0003238.1_g000025 Rmu_sc0003238.1_g000028 Rmu_sc0004719.1_g000009
rosa_roxburghii	Rroxscaffold_2G00146240
rosa_rugosa	Rorug02G0051800 Rorug02G0052600 Rorug05G0120200
rosa_samantha	Rh2AG099800 Rh2AG100400 Rh2BG101100 Rh2BG102200 Rh2CG103300 Rh2CG104600 Rh2DG100700 Rh2DG101500 Rh5AG211800 Rh5BG210700 Rh5CG234400 Rh5CG234500 Rh5DG216100
rosa_wichuraiana	Rw2G007570 Rw2G007630 Rw2G007640 Rw5G019420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1533
Acc16I	TGCGCA	1 cut(s)	249
Acc36I	ACCTGC	1 cut(s)	417
AccB1I	GGYRCC	1 cut(s)	2297
AccB7I	CCANNNNNTGG	1 cut(s)	1979
AccII	CGCG	1 cut(s)	2080
AciI	CCGC	2 cut(s)	678, 2078
AclWI	GGATC	4 cut(s)	46, 1424, 2076, 2290
AcoI	YGGCCR	3 cut(s)	179, 1955, 2424
AcsI	RAATTY	6 cut(s)	136, 423, 463, 2123, 2149, 2246
AcuI	CTGAAG	1 cut(s)	2289
AfaI	GTAC	2 cut(s)	166, 1400
AfiI	CCNNNNNNNGG	4 cut(s)	1281, 1628, 1979, 2335
AflIII	ACRYGT	3 cut(s)	695, 879, 1507
AjnI	CCWGG	1 cut(s)	294
AjuI	GAANNNNNNNTTGG	2 cut(s)	2189, 2221
AleI	CACNNNNGTG	1 cut(s)	175
Alw21I	GWGCWC	2 cut(s)	1773, 2363
Alw26I	GTCTC	2 cut(s)	221, 1051
AlwI	GGATC	4 cut(s)	46, 1424, 2076, 2290
Ama87I	CYCGRG	1 cut(s)	1295
AoxI	GGCC	5 cut(s)	179, 523, 1292, 1955, 2424
ApeKI	GCWGC	5 cut(s)	60, 914, 1083, 1370, 1526
ApoI	RAATTY	6 cut(s)	136, 423, 463, 2123, 2149, 2246
ArsI	GACNNNNNNTTYG	6 cut(s)	378, 410, 812, 844, 1790, 1822
Asp700I	GAANNNNTTC	3 cut(s)	258, 464, 1275
AspA2I	CCTAGG	1 cut(s)	1870
AspLEI	GCGC	1 cut(s)	250
AspS9I	GGNCC	4 cut(s)	353, 524, 1293, 1667
AsuC2I	CCSGG	1 cut(s)	1578
AsuHPI	GGTGA	4 cut(s)	100, 997, 1343, 1939
AsuII	TTCGAA	1 cut(s)	427
AsuNHI	GCTAGC	1 cut(s)	2252
AvaI	CYCGRG	1 cut(s)	1295
AvaII	GGWCC	2 cut(s)	353, 1667
AvrII	CCTAGG	1 cut(s)	1870
BalI	TGGCCA	2 cut(s)	181, 1957
BanI	GGYRCC	1 cut(s)	2297
BanII	GRGCYC	1 cut(s)	1773
BbsI	GAAGAC	4 cut(s)	229, 1307, 1531, 2029
Bbv12I	GWGCWC	2 cut(s)	1773, 2363
BbvCI	CCTCAGC	2 cut(s)	816, 1257
BbvI	GCAGC	5 cut(s)	72, 926, 1070, 1357, 1513
BccI	CCATC	8 cut(s)	89, 110, 113, 1973, 2058, 2146, 2300, 2333
BceAI	ACGGC	1 cut(s)	2411
BciT130I	CCWGG	1 cut(s)	296
BclI	TGATCA	1 cut(s)	1707
BcnI	CCSGG	1 cut(s)	1578
BcoDI	GTCTC	2 cut(s)	221, 1051
BfaI	CTAG	3 cut(s)	1383, 1871, 2253
BfmI	CTRYAG	2 cut(s)	58, 1227
BfuAI	ACCTGC	1 cut(s)	417
BisI	GCNGC	5 cut(s)	61, 915, 1084, 1371, 1527
BlnI	CCTAGG	1 cut(s)	1870
BlsI	GCNGC	5 cut(s)	62, 916, 1085, 1372, 1528
Bme1390I	CCNGG	2 cut(s)	296, 1578
Bme18I	GGWCC	2 cut(s)	353, 1667
BmeT110I	CYCGRG	1 cut(s)	1295
BmgT120I	GGNCC	4 cut(s)	353, 524, 1293, 1667
BmiI	GGNNCC	6 cut(s)	145, 401, 1575, 1971, 2299, 2392
BmrFI	CCNGG	2 cut(s)	296, 1578
BmsI	GCATC	6 cut(s)	208, 521, 574, 689, 937, 1369
BmtI	GCTAGC	1 cut(s)	2256
BoxI	GACNNNNGTC	1 cut(s)	233
BpiI	GAAGAC	4 cut(s)	229, 1307, 1531, 2029
BplI	GAGNNNNNCTC	2 cut(s)	2345, 2377
BpmI	CTGGAG	2 cut(s)	1677, 1956
Bpu10I	CCTNAGC	3 cut(s)	816, 1209, 1257
Bpu14I	TTCGAA	1 cut(s)	427
BpuEI	CTTGAG	1 cut(s)	501
BpuMI	CCSGG	1 cut(s)	1578
BsaJI	CCNNGG	8 cut(s)	647, 990, 1132, 1753, 1870, 2008, 2221, 2427
BsaXI	ACNNNNNCTCC	2 cut(s)	1494, 1524
Bsc4I	CCNNNNNNNGG	4 cut(s)	1281, 1628, 1979, 2335
Bse1I	ACTGG	8 cut(s)	257, 286, 611, 775, 1477, 1660, 1823, 1883
Bse3DI	GCAATG	2 cut(s)	1102, 1595
BseBI	CCWGG	1 cut(s)	296
BseDI	CCNNGG	8 cut(s)	647, 990, 1132, 1753, 1870, 2008, 2221, 2427
BseGI	GGATG	6 cut(s)	1267, 1312, 1432, 1984, 2098, 2325
BseLI	CCNNNNNNNGG	4 cut(s)	1281, 1628, 1979, 2335
BseMI	GCAATG	2 cut(s)	1102, 1595
BseMII	CTCAG	6 cut(s)	554, 807, 820, 1248, 1432, 1615
BseNI	ACTGG	8 cut(s)	257, 286, 611, 775, 1477, 1660, 1823, 1883
BseXI	GCAGC	5 cut(s)	72, 926, 1070, 1357, 1513
BseYI	CCCAGC	2 cut(s)	786, 1314
Bsh1236I	CGCG	1 cut(s)	2080
BshFI	GGCC	5 cut(s)	181, 525, 1294, 1957, 2426
BshNI	GGYRCC	1 cut(s)	2297
BsiHKAI	GWGCWC	2 cut(s)	1773, 2363
BsiHKCI	CYCGRG	1 cut(s)	1295
BsiSI	CCGG	1 cut(s)	1578
BslFI	GGGAC	1 cut(s)	2287
BslI	CCNNNNNNNGG	4 cut(s)	1281, 1628, 1979, 2335
BsmAI	GTCTC	2 cut(s)	221, 1051
BsmFI	GGGAC	1 cut(s)	2287
BsmI	GAATGC	1 cut(s)	250
BsnI	GGCC	5 cut(s)	181, 525, 1294, 1957, 2426
BsoBI	CYCGRG	1 cut(s)	1295
Bsp119I	TTCGAA	1 cut(s)	427
Bsp1286I	GDGCHC	2 cut(s)	1773, 2363
Bsp143I	GATC	6 cut(s)	51, 477, 1429, 1707, 2081, 2282
Bsp19I	CCATGG	3 cut(s)	647, 990, 1753
BspACI	CCGC	2 cut(s)	678, 2078
BspANI	GGCC	5 cut(s)	181, 525, 1294, 1957, 2426
BspCNI	CTCAG	6 cut(s)	553, 808, 819, 1249, 1431, 1614
BspFNI	CGCG	1 cut(s)	2080
BspHI	TCATGA	2 cut(s)	447, 973
BspLI	GGNNCC	6 cut(s)	145, 401, 1575, 1971, 2299, 2392
BspMAI	CTGCAG	1 cut(s)	62
BspMI	ACCTGC	1 cut(s)	417
BspOI	GCTAGC	1 cut(s)	2256
BspPI	GGATC	4 cut(s)	46, 1424, 2076, 2290
BspT104I	TTCGAA	1 cut(s)	427
BspT107I	GGYRCC	1 cut(s)	2297
BsrDI	GCAATG	2 cut(s)	1102, 1595
BsrI	ACTGG	8 cut(s)	257, 286, 611, 775, 1477, 1660, 1823, 1883
BssECI	CCNNGG	8 cut(s)	647, 990, 1132, 1753, 1870, 2008, 2221, 2427
BssMI	GATC	6 cut(s)	51, 477, 1429, 1707, 2081, 2282
BssT1I	CCWWGG	6 cut(s)	647, 990, 1132, 1753, 1870, 2221
Bst2UI	CCWGG	1 cut(s)	296
Bst4CI	ACNGT	7 cut(s)	419, 1057, 1231, 1612, 1705, 2009, 2045
Bst6I	CTCTTC	1 cut(s)	2411
BstAPI	GCANNNNNTGC	2 cut(s)	620, 1379
BstBI	TTCGAA	1 cut(s)	427
BstC8I	GCNNGC	3 cut(s)	2218, 2254, 2424
BstDEI	CTNAG	8 cut(s)	540, 747, 806, 816, 1209, 1257, 1418, 1601
BstDSI	CCRYGG	5 cut(s)	647, 990, 1753, 2008, 2427
BstENI	CCTNNNNNAGG	1 cut(s)	2333
BstF5I	GGATG	6 cut(s)	1267, 1312, 1432, 1984, 2098, 2325
BstFNI	CGCG	1 cut(s)	2080
BstHHI	GCGC	1 cut(s)	250
BstKTI	GATC	6 cut(s)	54, 480, 1432, 1710, 2084, 2285
BstMAI	GTCTC	2 cut(s)	221, 1051
BstMBI	GATC	6 cut(s)	51, 477, 1429, 1707, 2081, 2282
BstMWI	GCNNNNNNNGC	7 cut(s)	593, 620, 1092, 1379, 1954, 2226, 2432
BstNI	CCWGG	1 cut(s)	296
BstNSI	RCATGY	4 cut(s)	699, 883, 1511, 2220
BstPAI	GACNNNNGTC	1 cut(s)	233
BstSCI	CCNGG	2 cut(s)	294, 1576
BstSFI	CTRYAG	2 cut(s)	58, 1227
BstUI	CGCG	1 cut(s)	2080
BstV1I	GCAGC	5 cut(s)	72, 926, 1070, 1357, 1513
BstV2I	GAAGAC	4 cut(s)	229, 1307, 1531, 2029
BstXI	CCANNNNNNTGG	3 cut(s)	177, 1033, 1322
BsuRI	GGCC	5 cut(s)	181, 525, 1294, 1957, 2426
BtgI	CCRYGG	5 cut(s)	647, 990, 1753, 2008, 2427
BtsCI	GGATG	6 cut(s)	1267, 1312, 1432, 1984, 2098, 2325
BtsI	GCAGTG	1 cut(s)	1203
BtsIMutI	CAGTG	4 cut(s)	1053, 1203, 1236, 1609
BveI	ACCTGC	1 cut(s)	417
Cac8I	GCNNGC	3 cut(s)	2218, 2254, 2424
CciI	TCATGA	2 cut(s)	447, 973
CfoI	GCGC	1 cut(s)	250
Cfr13I	GGNCC	4 cut(s)	353, 524, 1293, 1667
CseI	GACGC	1 cut(s)	680
CsiI	ACCWGGT	1 cut(s)	294
Csp6I	GTAC	2 cut(s)	165, 1399
CspCI	CAANNNNNGTGG	2 cut(s)	1042, 1077
CviQI	GTAC	2 cut(s)	165, 1399
DdeI	CTNAG	8 cut(s)	540, 747, 806, 816, 1209, 1257, 1418, 1601
DpnI	GATC	6 cut(s)	53, 479, 1431, 1709, 2083, 2284
DpnII	GATC	6 cut(s)	51, 477, 1429, 1707, 2081, 2282
EaeI	YGGCCR	3 cut(s)	179, 1955, 2424
Eam1104I	CTCTTC	1 cut(s)	2411
EarI	CTCTTC	1 cut(s)	2411
Ecl136II	GAGCTC	1 cut(s)	1771
Eco130I	CCWWGG	6 cut(s)	647, 990, 1132, 1753, 1870, 2221
Eco24I	GRGCYC	1 cut(s)	1773
Eco47I	GGWCC	2 cut(s)	353, 1667
Eco53kI	GAGCTC	1 cut(s)	1771
Eco57I	CTGAAG	1 cut(s)	2289
Eco88I	CYCGRG	1 cut(s)	1295
EcoICRI	GAGCTC	1 cut(s)	1771
EcoNI	CCTNNNNNAGG	1 cut(s)	2333
EcoO109I	RGGNCCY	1 cut(s)	524
EcoRI	GAATTC	1 cut(s)	136
EcoRII	CCWGG	1 cut(s)	294
EcoT14I	CCWWGG	6 cut(s)	647, 990, 1132, 1753, 1870, 2221
EcoT22I	ATGCAT	1 cut(s)	2191
EcoT38I	GRGCYC	1 cut(s)	1773
ErhI	CCWWGG	6 cut(s)	647, 990, 1132, 1753, 1870, 2221
FalI	AAGNNNNNCTT	2 cut(s)	2021, 2053
FaqI	GGGAC	1 cut(s)	2287
FauI	CCCGC	1 cut(s)	685
FauNDI	CATATG	2 cut(s)	358, 2191
FbaI	TGATCA	1 cut(s)	1707
Fnu4HI	GCNGC	5 cut(s)	61, 915, 1084, 1371, 1527
FokI	GGATG	6 cut(s)	1274, 1319, 1439, 1991, 2105, 2312
FriOI	GRGCYC	1 cut(s)	1773
Fsp4HI	GCNGC	5 cut(s)	61, 915, 1084, 1371, 1527
FspAI	RTGCGCAY	1 cut(s)	249
FspBI	CTAG	3 cut(s)	1383, 1871, 2253
FspI	TGCGCA	1 cut(s)	249
GlaI	GCGC	1 cut(s)	249
GluI	GCNGC	5 cut(s)	61, 915, 1084, 1371, 1527
GsaI	CCCAGC	2 cut(s)	790, 1318
GsuI	CTGGAG	2 cut(s)	1677, 1956
HaeIII	GGCC	5 cut(s)	181, 525, 1294, 1957, 2426
HapII	CCGG	1 cut(s)	1578
HgaI	GACGC	1 cut(s)	680
HhaI	GCGC	1 cut(s)	250
Hin6I	GCGC	1 cut(s)	248
HinP1I	GCGC	1 cut(s)	248
HincII	GTYRAC	2 cut(s)	662, 1249
HindII	GTYRAC	2 cut(s)	662, 1249
HindIII	AAGCTT	1 cut(s)	1995
HinfI	GANTC	8 cut(s)	213, 316, 569, 626, 1271, 1749, 2197, 2354
HpaII	CCGG	1 cut(s)	1578
HphI	GGTGA	4 cut(s)	100, 997, 1343, 1939
Hpy188I	TCNGA	8 cut(s)	348, 543, 641, 1114, 1623, 1723, 2165, 2377
Hpy99I	CGWCG	1 cut(s)	267
HpyAV	CCTTC	7 cut(s)	120, 200, 320, 1286, 1808, 2264, 2339
HpyCH4III	ACNGT	7 cut(s)	419, 1057, 1231, 1612, 1705, 2009, 2045
HpyCH4IV	ACGT	5 cut(s)	84, 234, 822, 1536, 1548
HpyF10VI	GCNNNNNNNGC	7 cut(s)	593, 620, 1092, 1379, 1954, 2226, 2432
HpyF3I	CTNAG	8 cut(s)	540, 747, 806, 816, 1209, 1257, 1418, 1601
HpySE526I	ACGT	5 cut(s)	84, 234, 822, 1536, 1548
HspAI	GCGC	1 cut(s)	248
Ksp22I	TGATCA	1 cut(s)	1707
Kzo9I	GATC	6 cut(s)	51, 477, 1429, 1707, 2081, 2282
LmnI	GCTCC	4 cut(s)	405, 679, 1870, 1975
Lsp1109I	GCAGC	5 cut(s)	72, 926, 1070, 1357, 1513
LweI	GCATC	6 cut(s)	208, 521, 574, 689, 937, 1369
MabI	ACCWGGT	1 cut(s)	294
MaeI	CTAG	3 cut(s)	1383, 1871, 2253
MaeII	ACGT	5 cut(s)	84, 234, 822, 1536, 1548
MaeIII	GTNAC	6 cut(s)	713, 731, 1037, 1117, 1475, 2000
MalI	GATC	6 cut(s)	53, 479, 1431, 1709, 2083, 2284
MboI	GATC	6 cut(s)	51, 477, 1429, 1707, 2081, 2282
MboII	GAAGA	9 cut(s)	229, 335, 472, 1166, 1312, 1475, 1531, 2029, 2428
MfeI	CAATTG	3 cut(s)	222, 1676, 2364
MhlI	GDGCHC	2 cut(s)	1773, 2363
MlsI	TGGCCA	2 cut(s)	181, 1957
MluNI	TGGCCA	2 cut(s)	181, 1957
MlyI	GAGTC	3 cut(s)	207, 1743, 2363
MmeI	TCCRAC	5 cut(s)	1594, 1663, 1726, 2149, 2326
Mox20I	TGGCCA	2 cut(s)	181, 1957
Mph1103I	ATGCAT	1 cut(s)	2191
MroXI	GAANNNNTTC	3 cut(s)	258, 464, 1275
MscI	TGGCCA	2 cut(s)	181, 1957
MseI	TTAA	4 cut(s)	603, 1076, 1497, 2289
MslI	CAYNNNNRTG	2 cut(s)	175, 1491
Msp20I	TGGCCA	2 cut(s)	181, 1957
MspI	CCGG	1 cut(s)	1578
MspR9I	CCNGG	2 cut(s)	296, 1578
MunI	CAATTG	3 cut(s)	222, 1676, 2364
Mva1269I	GAATGC	1 cut(s)	250
MvaI	CCWGG	1 cut(s)	296
MvnI	CGCG	1 cut(s)	2080
MwoI	GCNNNNNNNGC	7 cut(s)	593, 620, 1092, 1379, 1954, 2226, 2432
NciI	CCSGG	1 cut(s)	1578
NcoI	CCATGG	3 cut(s)	647, 990, 1753
NdeI	CATATG	2 cut(s)	358, 2191
NdeII	GATC	6 cut(s)	51, 477, 1429, 1707, 2081, 2282
NheI	GCTAGC	1 cut(s)	2252
NlaIV	GGNNCC	6 cut(s)	145, 401, 1575, 1971, 2299, 2392
NmuCI	GTSAC	2 cut(s)	713, 2000
NsbI	TGCGCA	1 cut(s)	249
NsiI	ATGCAT	1 cut(s)	2191
NspI	RCATGY	4 cut(s)	699, 883, 1511, 2220
NspV	TTCGAA	1 cut(s)	427
OliI	CACNNNNGTG	1 cut(s)	175
PaeI	GCATGC	1 cut(s)	2220
PagI	TCATGA	2 cut(s)	447, 973
PciI	ACATGT	3 cut(s)	695, 879, 1507
PctI	GAATGC	1 cut(s)	250
PdmI	GAANNNNTTC	3 cut(s)	258, 464, 1275
PfeI	GAWTC	5 cut(s)	316, 569, 626, 1271, 2197
PflMI	CCANNNNNTGG	1 cut(s)	1979
PfoI	TCCNGGA	1 cut(s)	1576
PkrI	GCNGC	5 cut(s)	62, 916, 1085, 1372, 1528
PleI	GAGTC	3 cut(s)	207, 1743, 2362
PpsI	GAGTC	3 cut(s)	207, 1743, 2362
PscI	ACATGT	3 cut(s)	695, 879, 1507
PshAI	GACNNNNGTC	1 cut(s)	233
PsiI	TTATAA	1 cut(s)	1533
Psp124BI	GAGCTC	1 cut(s)	1773
Psp6I	CCWGG	1 cut(s)	294
PspFI	CCCAGC	2 cut(s)	786, 1314
PspGI	CCWGG	1 cut(s)	294
PspN4I	GGNNCC	6 cut(s)	145, 401, 1575, 1971, 2299, 2392
PspPI	GGNCC	4 cut(s)	353, 524, 1293, 1667
PstI	CTGCAG	1 cut(s)	62
RsaI	GTAC	2 cut(s)	166, 1400
RsaNI	GTAC	2 cut(s)	165, 1399
RseI	CAYNNNNRTG	2 cut(s)	175, 1491
SacI	GAGCTC	1 cut(s)	1773
SaqAI	TTAA	4 cut(s)	603, 1076, 1497, 2289
SatI	GCNGC	5 cut(s)	61, 915, 1084, 1371, 1527
Sau3AI	GATC	6 cut(s)	51, 477, 1429, 1707, 2081, 2282
Sau96I	GGNCC	4 cut(s)	353, 524, 1293, 1667
SchI	GAGTC	3 cut(s)	207, 1743, 2363
ScrFI	CCNGG	2 cut(s)	296, 1578
SduI	GDGCHC	2 cut(s)	1773, 2363
SexAI	ACCWGGT	1 cut(s)	294
SfaNI	GCATC	6 cut(s)	208, 521, 574, 689, 937, 1369
SfcI	CTRYAG	2 cut(s)	58, 1227
SfuI	TTCGAA	1 cut(s)	427
SinI	GGWCC	2 cut(s)	353, 1667
SmiMI	CAYNNNNRTG	2 cut(s)	175, 1491
SmlI	CTYRAG	1 cut(s)	516
SmoI	CTYRAG	1 cut(s)	516
SphI	GCATGC	1 cut(s)	2220
SsiI	CCGC	2 cut(s)	678, 2078
SspI	AATATT	1 cut(s)	1018
SspMI	CTAG	3 cut(s)	1383, 1871, 2253
SstI	GAGCTC	1 cut(s)	1773
StyD4I	CCNGG	2 cut(s)	294, 1576
StyI	CCWWGG	6 cut(s)	647, 990, 1132, 1753, 1870, 2221
TaaI	ACNGT	7 cut(s)	419, 1057, 1231, 1612, 1705, 2009, 2045
TaiI	ACGT	5 cut(s)	87, 237, 825, 1539, 1551
TaqI	TCGA	4 cut(s)	160, 216, 427, 1554
TatI	WGTACW	1 cut(s)	164
TfiI	GAWTC	5 cut(s)	316, 569, 626, 1271, 2197
Tru1I	TTAA	4 cut(s)	603, 1076, 1497, 2289
Tru9I	TTAA	4 cut(s)	603, 1076, 1497, 2289
TscAI	CASTG	4 cut(s)	1060, 1210, 1236, 1609
TseFI	GTSAC	2 cut(s)	713, 2000
TseI	GCWGC	5 cut(s)	60, 914, 1083, 1370, 1526
Tsp45I	GTSAC	2 cut(s)	713, 2000
TspGWI	ACGGA	1 cut(s)	1319
TspRI	CASTG	4 cut(s)	1060, 1210, 1236, 1609
Van91I	CCANNNNNTGG	1 cut(s)	1979
VpaK11BI	GGWCC	2 cut(s)	353, 1667
XagI	CCTNNNNNAGG	1 cut(s)	2333
XapI	RAATTY	6 cut(s)	136, 423, 463, 2123, 2149, 2246
XceI	RCATGY	4 cut(s)	699, 883, 1511, 2220
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1030
XmaJI	CCTAGG	1 cut(s)	1870
XmnI	GAANNNNTTC	3 cut(s)	258, 464, 1275
XspI	CTAG	3 cut(s)	1383, 1871, 2253
Zsp2I	ATGCAT	1 cut(s)	2191

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.