Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002228

Physical Location & Seq

Genomic Coordinates Forward (+)

10713807 .. 10724106

Gene Structure 10300 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G25170.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4155 bp

ATGAGTAGTTTGGTGGAGAGGCTTCGCATACGATCTGATAGGAAACCAGTTTATAACCTAGATGATTCTGATGATGACGACTTCGTTCCTAAAAAAGATCGAACCTTTGAGCAAGTCGAGGCTATTGTCAGAACTGATGCGAAAGAAAATGCATGTCAGGCTTGTGGGGAAAGTACTAATCTTGTAAGCTGCAATACATGCACTTATGCGTTCCATGCTAAATGCTTAGTTCCACCTCTTAAAGATGCTTCCGTGGAAAATTGGAGATGCCCTGAATGTGTTAGTCCTCTTAACGAGATAGATAAGATATTGGATTGTGAAATGCGTCCTACAAAATCTAGTGAACAAGGTTCCTCCGATGCGGAACCGAAGCCAATTTTTGTGAAACAGTATCTCGTGAAGTGGAAGGGATTATCATACCTTCACTGCTCTTGGGTGCCTGAGAAGGAGTTCCAGAAGGCTTATAAGTCAAATCATCGTTTAAAAACCAGAGTGAACAATTTTCACCGTCAAATGGAGTCATTCAATAACAGCGAAGATGATTTTGTTGCCATACGTCCTGAGTGGACCACTGTTGATCGGATTCTTGCCTGCAGAGAGGAAGATGGGGAGCTGGAATATCTTGTCAAATATAAAGAGCTATCCTATGATGAATGTTATTGGGAGTCAGAATCAGACATCTCAACCTTCCAGAATGAAATTCAAAGGTTCAAGGATGTAAATTCTAGAACTCGCAGAAGTAAAGATGTTGACCATAAAAGAAATCCCAGAGACTTTCAACAGTTTGATCATACTCCTGAATTCCTCAAAGGCTTGTTACATCCATACCAGCTTGAGGGACTTAATTTTTTGCGGTTCTCGTGGTCAAAACAGACGCATGTAATCCTTGCTGATGAAATGGGACTAGGCAAGACAATTCAAAGCATTGCCCTTTTAGCTTCACTTTTTGAGGAGAACCTCATTCCGCATTTGGTAATTGCTCCTCTATCGACTCTGCGTAACTGGGAGAGAGAGTTTGCCACATGGGCCCCACAGATGAACGTGGTTATGTATTTTGGCACTGCGCAAGCTCGAGCAGTTATCAGAGAACATGAGTTTTACTTATCGAAAGATCAAAAAAAGATCAAGAAAAAGAAATCTGGACAAATAAGTAGCGAAAGCAAGCAAAAAAGAATCAAGTTTGATGTCCTCCTCACATCGTATGAGATGATCAACCTAGATTCAGCAGTTCTAAAACCAATTAAGTGGGAGTGCATGATTGTTGATGAAGGTCATCGACTGAAAAATAAGGATTCAAAGCTGTTCTCTTCATTGACACAGTATTCAAGTAACCACCGTATTCTTCTGACAGGAACACCACTTCAGAACAACTTGGATGAACTTTTCATGCTCATGCATTTTCTTGATGCGGGGAAGTTTGGAAGTTTGGAGGAGTTCCAGGAGGAGTTCAAAGATATTAATCAAGAGGAGCAGATCTCAAGGTTGCACAAAATGTTGGCTCCACATTTGCTCAGAAGGGTAAAAAAAGACGTAATGAAAGACATGCCCCCCAAAAAGGAGCTCATTTTGCGTGTTGATCTGAGCAGTCTGCAGAAAGAATATTACAAAGCTATTTTTACCCGTAATTATCAAGTATTGACAAAAAAGGGAGGTGCTCAAATTTCCCTTAATAACATTATGATGGAATTACGAAAAGTATGCTGCCATCCTTATATGCTAGAGGGTGTTGAGCCAGTTATTCACGACGCAAATGAAGCTTTCAAACAACTTTTGGAGTCTTGTGGAAAGCTGCAACTTCTAGATAAAATGATGGTCAAACTGAAAGAGCAAGGACACAGAGTCCTAATATACACACAGTTTCAGCATATGCTGGACTTACTTGAAGACTACTGTACCCATAAGAAATGGCAGTACGAGCGAATTGATGGAAAGGTTGGCGGAGCTGAGCGGCAAATACGCATAGATCGGTTCAATGCCAAAAATTCTAACAAGTTTTGTTTTTTGCTCTCCACAAGAGCTGGTGGCTTAGGAATAAATCTTGCAACGGCTGATACAGTAATCATTTATGACAGTGACTGGAATCCTCATGCTGATCTTCAAGCAATGGCTAGAGCTCATCGACTTGGCCAAACAAATAAGGTGATGATTTATAGGCTCATAAACCGAGGCACCATTGAAGAAAGGATGATGCAATTGACTAAAAAGAAAATGGTTCTAGAGCATCTTGTTGTTGGGAAACTCAAAACACAAAACATTAATCAGGAAGAGTTAGATGACATCATCAGGTATGGATCAAAGGAGCTTTTTGCTAGTGAAGATGATGAAGCAGGAAAGTCTGGAAAAATTCATTATGATGATGCGGCTATAGACAAATTGCTTGATCGTGATCTCGTGGAGGCAGAGGAAGTCTCAGTGGATGATGAAGAGGAGAATGGATTCTTAAAGGCTTTCAAGGTGGCTAATTTTGAATATATAGATGAAAATGAGGCAGCAGCATTAGAGGCACAGAGAGTCGCTGCTGAAAGCAAATCTTCAGCAGGCAATTCTGATAGAGCAAGTTATTGGGAAGAGTTGTTAAAAGATAAATTTGAGCTGCACCAGGCTGAGGAGCTTAATGCTCTTGGAAAAAGGAAGAGAAGTCGCAAGCAGTTGGTATCCATTGAAGAAGATGATCTTGCTGGTTTGGAAGATGTGAGCTCTGATGGAGATGAAAGTTATGAAGCTGAGTCAACAGATGGTGAAGCAGCAGGACAAGGAGTTCAGACGGGTCGACGGCCGTACAGAAGAAAGGGTCGCGATAATTTGGAACCAACTCCGTTGATGGAAGGTGAGGGGAGATCTTTCAGAGTACTGGGTTTCAACCAGAGTCAAAGGGCCATTTTTGTACAGACTTTGATGAGGTATGGAGCTGGCAATTTTGATTGGAAGGAGTTTGTTCCTCGCTTAAAGCAGAAGACCTTTGAAGAAATAAATGAATATGGAATACTCTTCTTGAAGCACATTGCTGAAGAAATAGACGAGAATTCTCCAACCTTTTCAGATGGTGTGCCCAAGGAAGGACTTAGAATAGAAGATGTTCTAGTCAGAATTGCTCTTCTGATACTAGTTCAGGAGAAGGTGAAATTTGTAGAAGATCATCCAGGGAAACCTGTTTTCCCCTCTCGCATTCTTGAAAGATTCCCCGGACTGAGAAGTGGAAAAATTTGGAAGGAGGAACATGACAAGATAATGATACGTGCTGTTTTAAAGCATGGGTACGGACGGTGGCAAGCTATTGTTGATGACAAAGAGTTGGGGATCCAAGAGCTTATCTGCAAAGAATTGAATTTCCCTCACATAAGTTTGTCTGCTGCTGAACAAGCTGGTTTGCAGGGGCAGAATGGTAGTGGGGGCTCTAATCCGGGAGCACAGACTAACCAGAATCCTGGAAGCGTTATTACTGGGAACAATAATGCTTCTGCTGATGGGGCTCAAGTAAACTCGATGTTCTATTATCGGGACATGCAGAGACGACTTGTTGAGTTTGTGAAAAAGCGAGTTCTGCTTTTGGAGAAGGCGATGAATTATGAATACGCAGAGGAATATTATGGACTTGGTGGCTCATCATCTATCCCTACTGAAGAACCAGAAGCTGAACCAAAGATCGCTGACACAGTGGGAGTGAGCTTTATTGAGGTTGATGATGAAATGCTTGATGGACTTCCTAAGACTGATCCTATCACTTCAGAAGAAATTATGGGGGCTGCTGTTGACAACAACCAAGCGCGGGTCGAAATAGCTCAACATTATAACCAGATGTGCAAACTTCTTGATGAGAACGCTCGGGAATCAGTCCAAGCATATGTAAACAACCAACCACCGAGTACCAAGGTGAATGAGAGCTTCCGTGCACTCAAATCTATCAATGGTAACATTAACACAATCCTTTCGATTACATCTGATCAATCCAAGTCACATGAAGACGACACCAAGCCAGACCTAAACAATGTTGAGATGAAGGACACGGCCGAAGAAACAAAACCGTTAAGAGGTGGCGTCGTCGATCTGAATGTGGTGGAGGGAGAGGAGAACATTGCTGAAGCTAGTGGAAGTGTTGATGTAAAAATGGAAGAAGCCAAAGAAGAAGAGAAGCCAAAGAACATGGTCGTTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006338 GO:0006355 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008283 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009736 GO:0009739 GO:0009755 GO:0009787 GO:0009788 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016043 GO:0019219 GO:0019222 GO:0022622 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048364 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071368 GO:0071495 GO:0071840 GO:0080090 GO:0097159 GO:0099402 GO:1901363 GO:1901419 GO:1901420 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:1905957 GO:1905958 GO:2000023 GO:2000026 GO:2000069 GO:2000112 GO:2000113 GO:2000280 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko05165 ko05203 map05165 map05203

Pfam Domains

Protein Families

CHDCT2 Chromo DUF1086 DUF1087 Helicase_C PHD SNF2_N

Protein Analysis

1384

Amino Acids

158.4

Weight (kDa)

5.43

Isoelectric Point (pI)

41.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PHD	PF00628	78 - 119	1.1e-07	PHD-finger
Chromo	PF00385	125 - 180	1.1e-08	Chromo (CHRromatin Organisation MOdifier) domain
Chromo	PF00385	217 - 265	1.1e-14	Chromo (CHRromatin Organisation MOdifier) domain
ResIII	PF04851	299 - 479	4.6e-10	Type III restriction enzyme, res subunit
SNF2-rel_dom	PF00176	302 - 598	4.3e-83	SNF2-related domain
HDA2-3	PF11496	549 - 764	5.4e-10	Class II histone deacetylase complex subunits 2 and 3
Helicase_C	PF00271	622 - 735	6.9e-25	Helicase conserved C-terminal domain
DUF1087	PF06465	856 - 917	5.2e-24	CHD subfamily II, DUF1087
CHDII_SANT-like	PF06461	947 - 1080	2.9e-62	CHD subfamily II, SANT-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002228)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25170 AT2G25170 AT2G25170 AT2G25170 AT4G31900 AT4G31900 AT4G31900
fragaria_vesca	FvH4_2g40920
malus_domestica	MD08G1058800.v1.1 MD15G1037700.v1.1
prunus_persica	Prupe.1G391600_v2.0.a1
pyrus_communis	pycom08g04860 pycom08g04870 pycom15g03490
rosa_chinensis	RchiOBHm_Chr3g0455421 RchiOBHm_Chr6g0299661
rosa_laevigata	RLG00000011399 RLG00000025364
rosa_multiflora	Rmu_co8019708.1_g000001 Rmu_co8099568.1_g000001 Rmu_sc0022216.1_g000002 Rmu_sc0024261.1_g000001 Rmu_sc0024262.1_g000001
rosa_roxburghii	Rroxscaffold_6G00424300 Rroxscaffold_7G00168470
rosa_rugosa	Rorug03G0007200 Rorug03G0007300 Rorug03G0007400 Rorug04G0013500 Rorug06G0291400
rosa_samantha	Rh3AG066700 Rh3BG069100 Rh3CG067500 Rh3DG069000 Rh5AG515500 Rh6AG402100 Rh6BG410600 Rh6CG416300 Rh6CG416400 Rh6DG403500
rosa_wichuraiana	Rw2G021110 Rw3G005270 Rw6G035200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	54, 465, 3789
Acc16I	TGCGCA	1 cut(s)	1065
AccB1I	GGYRCC	2 cut(s)	436, 2168
AccBSI	CCGCTC	1 cut(s)	1948
AccI	GTMKAC	1 cut(s)	2770
AccII	CGCG	2 cut(s)	2796, 3766
AciI	CCGC	8 cut(s)	362, 853, 965, 1409, 1938, 1948, 2360, 3766
AclWI	GGATC	4 cut(s)	2299, 3292, 3305, 3707
AcoI	YGGCCR	3 cut(s)	2125, 2774, 4005
AcuI	CTGAAG	6 cut(s)	1346, 2517, 3027, 3639, 3708, 4098
AcyI	GRCGYC	1 cut(s)	4035
AfaI	GTAC	8 cut(s)	175, 1894, 1913, 2780, 2850, 2886, 3257, 3865
AfiI	CCNNNNNNNGG	7 cut(s)	514, 835, 1555, 2605, 3056, 3766, 4028
AhdI	GACNNNNNGTC	1 cut(s)	1838
AhlI	ACTAGT	1 cut(s)	3103
AjnI	CCWGG	4 cut(s)	1437, 2598, 3139, 3424
AloI	GAACNNNNNNTCC	2 cut(s)	2742, 2774
Alw21I	GWGCWC	6 cut(s)	1563, 1657, 2116, 2699, 3409, 3892
Alw26I	GTCTC	3 cut(s)	765, 2413, 3502
Alw44I	GTGCAC	1 cut(s)	3888
AlwI	GGATC	4 cut(s)	2299, 3292, 3305, 3707
AlwNI	CAGNNNCTG	2 cut(s)	2076, 3632
Ama87I	CYCGRG	2 cut(s)	1071, 3822
AoxI	GGCC	5 cut(s)	1026, 2125, 2774, 2874, 4005
ApaI	GGGCCC	1 cut(s)	1030
ApaLI	GTGCAC	1 cut(s)	3888
AseI	ATTAAT	2 cut(s)	1458, 2256
Asp700I	GAANNNNTTC	3 cut(s)	449, 2435, 3075
AspLEI	GCGC	2 cut(s)	1066, 3766
AspS9I	GGNCC	4 cut(s)	567, 1026, 1027, 2874
AsuC2I	CCSGG	2 cut(s)	3183, 3402
AsuHPI	GGTGA	6 cut(s)	497, 2152, 2750, 2840, 3130, 3883
AvaI	CYCGRG	2 cut(s)	1071, 3822
AvaII	GGWCC	1 cut(s)	567
BaeGI	GKGCMC	3 cut(s)	1030, 3051, 3892
BaeI	ACNNNNGTAYC	2 cut(s)	1876, 1909
BalI	TGGCCA	1 cut(s)	2127
BamHI	GGATCC	1 cut(s)	3297
BanI	GGYRCC	2 cut(s)	436, 2168
BanII	GRGCYC	6 cut(s)	1030, 1563, 2116, 2699, 3395, 3472
BarI	GAAGNNNNNNTAC	2 cut(s)	3421, 3453
BauI	CACGAG	3 cut(s)	395, 859, 2390
BbsI	GAAGAC	3 cut(s)	1890, 2960, 3966
Bbv12I	GWGCWC	6 cut(s)	1563, 1657, 2116, 2699, 3409, 3892
BbvCI	CCTCAGC	1 cut(s)	2604
BceAI	ACGGC	4 cut(s)	2061, 2761, 2789, 4020
BcgI	CGANNNNNNTGC	2 cut(s)	1680, 1714
BciT130I	CCWGG	4 cut(s)	1439, 2600, 3141, 3426
BciVI	GTATCC	1 cut(s)	2665
BclI	TGATCA	3 cut(s)	787, 1209, 3940
BcnI	CCSGG	2 cut(s)	3183, 3402
BcoDI	GTCTC	3 cut(s)	765, 2413, 3502
BcuI	ACTAGT	1 cut(s)	3103
BfmI	CTRYAG	3 cut(s)	592, 1589, 2364
BfuI	GTATCC	1 cut(s)	2665
BglI	GCCNNNNNGGC	1 cut(s)	1025
BglII	AGATCT	2 cut(s)	1473, 2837
BlpI	GCTNAGC	1 cut(s)	1944
BmcAI	AGTACT	2 cut(s)	175, 2850
Bme1390I	CCNGG	6 cut(s)	1439, 2600, 3141, 3183, 3402, 3426
Bme18I	GGWCC	1 cut(s)	567
BmeRI	GACNNNNNGTC	1 cut(s)	1838
BmeT110I	CYCGRG	2 cut(s)	1071, 3822
BmgT120I	GGNCC	4 cut(s)	567, 1026, 1027, 2874
BmiI	GGNNCC	9 cut(s)	352, 366, 438, 1028, 1029, 1500, 2170, 2808, 3299
BmrFI	CCNGG	6 cut(s)	1439, 2600, 3141, 3183, 3402, 3426
BmrI	ACTGGG	3 cut(s)	1012, 2861, 3450
BmsI	GCATC	8 cut(s)	127, 235, 257, 349, 1396, 2178, 2230, 2347
BmuI	ACTGGG	3 cut(s)	1012, 2861, 3450
BpiI	GAAGAC	3 cut(s)	1890, 2960, 3966
BplI	GAGNNNNNCTC	4 cut(s)	1460, 1492, 2393, 2425
Bpu10I	CCTNAGC	2 cut(s)	2026, 2604
Bpu1102I	GCTNAGC	1 cut(s)	1944
BpuEI	CTTGAG	3 cut(s)	854, 1462, 3456
BpuMI	CCSGG	2 cut(s)	3183, 3402
BsaAI	YACGTR	1 cut(s)	3236
BsaBI	GATNNNNATC	2 cut(s)	1458, 2385
BsaHI	GRCGYC	1 cut(s)	4035
BsaJI	CCNNGG	6 cut(s)	252, 2164, 3051, 3140, 3181, 3867
BsaXI	ACNNNNNCTCC	2 cut(s)	998, 1028
Bsc4I	CCNNNNNNNGG	7 cut(s)	514, 835, 1555, 2605, 3056, 3766, 4028
Bse1I	ACTGG	6 cut(s)	47, 1007, 1733, 2081, 2856, 3445
Bse3DI	GCAATG	4 cut(s)	924, 2109, 3000, 4071
Bse8I	GATNNNNATC	2 cut(s)	1458, 2385
BseBI	CCWGG	4 cut(s)	1439, 2600, 3141, 3426
BseDI	CCNNGG	6 cut(s)	252, 2164, 3051, 3140, 3181, 3867
BseGI	GGATG	7 cut(s)	721, 820, 1381, 1705, 2190, 2422, 3136
BseJI	GATNNNNATC	2 cut(s)	1458, 2385
BseLI	CCNNNNNNNGG	7 cut(s)	514, 835, 1555, 2605, 3056, 3766, 4028
BseMI	GCAATG	4 cut(s)	924, 2109, 3000, 4071
BseMII	CTCAG	9 cut(s)	432, 552, 1525, 1571, 1935, 2424, 2595, 2715, 3179
BseNI	ACTGG	6 cut(s)	47, 1007, 1733, 2081, 2856, 3445
BseRI	GAGGAG	9 cut(s)	965, 972, 1181, 1445, 1457, 1481, 2441, 2621, 4079
BseSI	GKGCMC	3 cut(s)	1030, 3051, 3892
BseX3I	CGGCCG	2 cut(s)	2774, 4005
BsgI	GTGCAG	1 cut(s)	2579
Bsh1236I	CGCG	2 cut(s)	2796, 3766
Bsh1285I	CGRYCG	2 cut(s)	2777, 4008
BshFI	GGCC	5 cut(s)	1028, 2127, 2776, 2876, 4007
BshNI	GGYRCC	2 cut(s)	436, 2168
BsiEI	CGRYCG	2 cut(s)	2777, 4008
BsiHKAI	GWGCWC	6 cut(s)	1563, 1657, 2116, 2699, 3409, 3892
BsiHKCI	CYCGRG	2 cut(s)	1071, 3822
BsiSI	CCGG	2 cut(s)	3183, 3401
BslFI	GGGAC	3 cut(s)	852, 915, 3512
BslI	CCNNNNNNNGG	7 cut(s)	514, 835, 1555, 2605, 3056, 3766, 4028
BsmAI	GTCTC	3 cut(s)	765, 2413, 3502
BsmBI	CGTCTC	1 cut(s)	3502
BsmFI	GGGAC	3 cut(s)	852, 915, 3512
BsmI	GAATGC	1 cut(s)	3165
BsnI	GGCC	5 cut(s)	1028, 2127, 2776, 2876, 4007
BsoBI	CYCGRG	2 cut(s)	1071, 3822
Bsp120I	GGGCCC	1 cut(s)	1026
Bsp1407I	TGTACA	1 cut(s)	2884
Bsp1720I	GCTNAGC	1 cut(s)	1944
Bsp68I	TCGCGA	1 cut(s)	2796
BspACI	CCGC	8 cut(s)	362, 853, 965, 1409, 1938, 1948, 2360, 3766
BspANI	GGCC	5 cut(s)	1028, 2127, 2776, 2876, 4007
BspCNI	CTCAG	9 cut(s)	433, 553, 1524, 1572, 1936, 2423, 2596, 2716, 3180
BspFNI	CGCG	2 cut(s)	2796, 3766
BspLI	GGNNCC	9 cut(s)	352, 366, 438, 1028, 1029, 1500, 2170, 2808, 3299
BspMAI	CTGCAG	2 cut(s)	596, 1593
BspPI	GGATC	4 cut(s)	2299, 3292, 3305, 3707
BspQI	GCTCTTC	1 cut(s)	3099
BspT107I	GGYRCC	2 cut(s)	436, 2168
BsrBI	CCGCTC	1 cut(s)	1948
BsrDI	GCAATG	4 cut(s)	924, 2109, 3000, 4071
BsrGI	TGTACA	1 cut(s)	2884
BsrI	ACTGG	6 cut(s)	47, 1007, 1733, 2081, 2856, 3445
BssECI	CCNNGG	6 cut(s)	252, 2164, 3051, 3140, 3181, 3867
BssNI	GRCGYC	1 cut(s)	4035
BssSI	CACGAG	3 cut(s)	395, 859, 2390
BssT1I	CCWWGG	2 cut(s)	3051, 3867
Bst2BI	CACGAG	3 cut(s)	395, 859, 2390
Bst2UI	CCWGG	4 cut(s)	1439, 2600, 3141, 3426
Bst6I	CTCTTC	8 cut(s)	1312, 2259, 2418, 2562, 2627, 2993, 3099, 4119
BstACI	GRCGYC	1 cut(s)	4035
BstAPI	GCANNNNNTGC	1 cut(s)	198
BstAUI	TGTACA	1 cut(s)	2884
BstBAI	YACGTR	1 cut(s)	3236
BstC8I	GCNNGC	7 cut(s)	592, 1068, 1163, 2539, 2645, 2911, 3270
BstDSI	CCRYGG	1 cut(s)	252
BstF5I	GGATG	7 cut(s)	721, 820, 1381, 1705, 2190, 2422, 3136
BstFNI	CGCG	2 cut(s)	2796, 3766
BstHHI	GCGC	2 cut(s)	1066, 3766
BstMAI	GTCTC	3 cut(s)	765, 2413, 3502
BstMCI	CGRYCG	2 cut(s)	2777, 4008
BstNI	CCWGG	4 cut(s)	1439, 2600, 3141, 3426
BstNSI	RCATGY	5 cut(s)	156, 201, 881, 1546, 3505
BstSCI	CCNGG	6 cut(s)	1437, 2598, 3139, 3181, 3400, 3424
BstSFI	CTRYAG	3 cut(s)	592, 1589, 2364
BstSLI	GKGCMC	3 cut(s)	1030, 3051, 3892
BstUI	CGCG	2 cut(s)	2796, 3766
BstV2I	GAAGAC	3 cut(s)	1890, 2960, 3966
BstX2I	RGATCY	3 cut(s)	1473, 2837, 3297
BstXI	CCANNNNNNTGG	2 cut(s)	1245, 3425
BstYI	RGATCY	3 cut(s)	1473, 2837, 3297
BstZI	CGGCCG	2 cut(s)	2774, 4005
BsuI	GTATCC	1 cut(s)	2665
BsuRI	GGCC	5 cut(s)	1028, 2127, 2776, 2876, 4007
BtgI	CCRYGG	1 cut(s)	252
BtgZI	GCGATG	1 cut(s)	3572
BtsCI	GGATG	7 cut(s)	721, 820, 1381, 1705, 2190, 2422, 3136
BtsI	GCAGTG	2 cut(s)	424, 1059
BtsIMutI	CAGTG	6 cut(s)	424, 570, 1059, 2077, 2418, 3660
BtuMI	TCGCGA	1 cut(s)	2796
Cac8I	GCNNGC	7 cut(s)	592, 1068, 1163, 2539, 2645, 2911, 3270
CaiI	CAGNNNCTG	2 cut(s)	2076, 3632
CfoI	GCGC	2 cut(s)	1066, 3766
Cfr13I	GGNCC	4 cut(s)	567, 1026, 1027, 2874
CseI	GACGC	4 cut(s)	314, 883, 1754, 4024
Csp6I	GTAC	8 cut(s)	174, 1893, 1912, 2779, 2849, 2885, 3256, 3864
CviQI	GTAC	8 cut(s)	174, 1893, 1912, 2779, 2849, 2885, 3256, 3864
DraI	TTTAAA	2 cut(s)	483, 3246
DriI	GACNNNNNGTC	1 cut(s)	1838
EaeI	YGGCCR	3 cut(s)	2125, 2774, 4005
EagI	CGGCCG	2 cut(s)	2774, 4005
Eam1104I	CTCTTC	8 cut(s)	1312, 2259, 2418, 2562, 2627, 2993, 3099, 4119
Eam1105I	GACNNNNNGTC	1 cut(s)	1838
EarI	CTCTTC	8 cut(s)	1312, 2259, 2418, 2562, 2627, 2993, 3099, 4119
EciI	GGCGGA	1 cut(s)	1953
Ecl136II	GAGCTC	3 cut(s)	1561, 2114, 2697
EclXI	CGGCCG	2 cut(s)	2774, 4005
Eco130I	CCWWGG	2 cut(s)	3051, 3867
Eco24I	GRGCYC	6 cut(s)	1030, 1563, 2116, 2699, 3395, 3472
Eco47I	GGWCC	1 cut(s)	567
Eco52I	CGGCCG	2 cut(s)	2774, 4005
Eco53kI	GAGCTC	3 cut(s)	1561, 2114, 2697
Eco57I	CTGAAG	6 cut(s)	1346, 2517, 3027, 3639, 3708, 4098
Eco88I	CYCGRG	2 cut(s)	1071, 3822
EcoICRI	GAGCTC	3 cut(s)	1561, 2114, 2697
EcoO109I	RGGNCCY	1 cut(s)	1027
EcoRI	GAATTC	2 cut(s)	800, 3022
EcoRII	CCWGG	4 cut(s)	1437, 2598, 3139, 3424
EcoT14I	CCWWGG	2 cut(s)	3051, 3867
EcoT22I	ATGCAT	2 cut(s)	154, 1398
EcoT38I	GRGCYC	6 cut(s)	1030, 1563, 2116, 2699, 3395, 3472
ErhI	CCWWGG	2 cut(s)	3051, 3867
Esp3I	CGTCTC	1 cut(s)	3502
FalI	AAGNNNNNCTT	4 cut(s)	2515, 2547, 2658, 2690
FaqI	GGGAC	3 cut(s)	852, 915, 3512
FauI	CCCGC	2 cut(s)	1402, 3759
FauNDI	CATATG	2 cut(s)	1866, 3841
FbaI	TGATCA	3 cut(s)	787, 1209, 3940
FblI	GTMKAC	1 cut(s)	2770
FokI	GGATG	7 cut(s)	728, 807, 1388, 1692, 2197, 2429, 3123
FriOI	GRGCYC	6 cut(s)	1030, 1563, 2116, 2699, 3395, 3472
FspI	TGCGCA	1 cut(s)	1065
GlaI	GCGC	2 cut(s)	1065, 3765
HaeIII	GGCC	5 cut(s)	1028, 2127, 2776, 2876, 4007
HapII	CCGG	2 cut(s)	3183, 3401
HgaI	GACGC	4 cut(s)	314, 883, 1754, 4024
HhaI	GCGC	2 cut(s)	1066, 3766
Hin1I	GRCGYC	1 cut(s)	4035
Hin6I	GCGC	2 cut(s)	1064, 3764
HinP1I	GCGC	2 cut(s)	1064, 3764
HincII	GTYRAC	4 cut(s)	751, 2730, 2771, 3751
HindII	GTYRAC	4 cut(s)	751, 2730, 2771, 3751
HindIII	AAGCTT	1 cut(s)	1755
HpaII	CCGG	2 cut(s)	3183, 3401
HphI	GGTGA	6 cut(s)	497, 2152, 2750, 2840, 3130, 3883
Hpy99I	CGWCG	4 cut(s)	1748, 2775, 4040, 4043
HpyCH4IV	ACGT	4 cut(s)	556, 1041, 1530, 3235
HpySE526I	ACGT	4 cut(s)	556, 1041, 1530, 3235
Hsp92I	GRCGYC	1 cut(s)	4035
HspAI	GCGC	2 cut(s)	1064, 3764
Ksp22I	TGATCA	3 cut(s)	787, 1209, 3940
LguI	GCTCTTC	1 cut(s)	3099
LweI	GCATC	8 cut(s)	127, 235, 257, 349, 1396, 2178, 2230, 2347
MaeII	ACGT	4 cut(s)	556, 1041, 1530, 3235
MaeIII	GTNAC	6 cut(s)	816, 998, 1328, 2072, 3908, 3951
MbiI	CCGCTC	1 cut(s)	1948
MfeI	CAATTG	1 cut(s)	2192
MflI	RGATCY	3 cut(s)	1473, 2837, 3297
MlsI	TGGCCA	1 cut(s)	2127
MluNI	TGGCCA	1 cut(s)	2127
MlyI	GAGTC	8 cut(s)	527, 674, 985, 1784, 1848, 2520, 2735, 2875
MmeI	TCCRAC	1 cut(s)	3053
Mox20I	TGGCCA	1 cut(s)	2127
Mph1103I	ATGCAT	2 cut(s)	154, 1398
MroXI	GAANNNNTTC	3 cut(s)	449, 2435, 3075
MscI	TGGCCA	1 cut(s)	2127
MslI	CAYNNNNRTG	3 cut(s)	1391, 1679, 2352
Msp20I	TGGCCA	1 cut(s)	2127
MspI	CCGG	2 cut(s)	3183, 3401
MspR9I	CCNGG	6 cut(s)	1439, 2600, 3141, 3183, 3402, 3426
MunI	CAATTG	1 cut(s)	2192
Mva1269I	GAATGC	1 cut(s)	3165
MvaI	CCWGG	4 cut(s)	1439, 2600, 3141, 3426
MvnI	CGCG	2 cut(s)	2796, 3766
NciI	CCSGG	2 cut(s)	3183, 3402
NdeI	CATATG	2 cut(s)	1866, 3841
NlaIV	GGNNCC	9 cut(s)	352, 366, 438, 1028, 1029, 1500, 2170, 2808, 3299
NmuCI	GTSAC	2 cut(s)	2072, 3951
NruI	TCGCGA	1 cut(s)	2796
NsbI	TGCGCA	1 cut(s)	1065
NsiI	ATGCAT	2 cut(s)	154, 1398
NspI	RCATGY	5 cut(s)	156, 201, 881, 1546, 3505
PaeR7I	CTCGAG	1 cut(s)	1071
PciSI	GCTCTTC	1 cut(s)	3099
PctI	GAATGC	1 cut(s)	3165
PdmI	GAANNNNTTC	3 cut(s)	449, 2435, 3075
PfoI	TCCNGGA	3 cut(s)	1437, 3400, 3424
PleI	GAGTC	8 cut(s)	526, 673, 985, 1783, 1847, 2519, 2734, 2874
PpsI	GAGTC	8 cut(s)	526, 673, 985, 1783, 1847, 2519, 2734, 2874
Ppu21I	YACGTR	1 cut(s)	3236
PshBI	ATTAAT	2 cut(s)	1458, 2256
PsiI	TTATAA	3 cut(s)	54, 465, 3789
Psp124BI	GAGCTC	3 cut(s)	1563, 2116, 2699
Psp6I	CCWGG	4 cut(s)	1437, 2598, 3139, 3424
PspGI	CCWGG	4 cut(s)	1437, 2598, 3139, 3424
PspN4I	GGNNCC	9 cut(s)	352, 366, 438, 1028, 1029, 1500, 2170, 2808, 3299
PspOMI	GGGCCC	1 cut(s)	1026
PspPI	GGNCC	4 cut(s)	567, 1026, 1027, 2874
PspXI	VCTCGAGB	1 cut(s)	1071
PstI	CTGCAG	2 cut(s)	596, 1593
PstNI	CAGNNNCTG	2 cut(s)	2076, 3632
PsuI	RGATCY	3 cut(s)	1473, 2837, 3297
RruI	TCGCGA	1 cut(s)	2796
RsaI	GTAC	8 cut(s)	175, 1894, 1913, 2780, 2850, 2886, 3257, 3865
RsaNI	GTAC	8 cut(s)	174, 1893, 1912, 2779, 2849, 2885, 3256, 3864
RseI	CAYNNNNRTG	3 cut(s)	1391, 1679, 2352
SacI	GAGCTC	3 cut(s)	1563, 2116, 2699
SalI	GTCGAC	1 cut(s)	2769
SapI	GCTCTTC	1 cut(s)	3099
Sau96I	GGNCC	4 cut(s)	567, 1026, 1027, 2874
ScaI	AGTACT	2 cut(s)	175, 2850
SchI	GAGTC	8 cut(s)	527, 674, 985, 1784, 1848, 2520, 2735, 2875
ScrFI	CCNGG	6 cut(s)	1439, 2600, 3141, 3183, 3402, 3426
SfaNI	GCATC	8 cut(s)	127, 235, 257, 349, 1396, 2178, 2230, 2347
SfcI	CTRYAG	3 cut(s)	592, 1589, 2364
Sfr274I	CTCGAG	1 cut(s)	1071
SinI	GGWCC	1 cut(s)	567
SlaI	CTCGAG	1 cut(s)	1071
SmiMI	CAYNNNNRTG	3 cut(s)	1391, 1679, 2352
SmlI	CTYRAG	4 cut(s)	833, 1071, 1477, 3471
SmoI	CTYRAG	4 cut(s)	833, 1071, 1477, 3471
SpeI	ACTAGT	1 cut(s)	3103
SsiI	CCGC	8 cut(s)	362, 853, 965, 1409, 1938, 1948, 2360, 3766
SspI	AATATT	2 cut(s)	1601, 3584
SstI	GAGCTC	3 cut(s)	1563, 2116, 2699
StyD4I	CCNGG	6 cut(s)	1437, 2598, 3139, 3181, 3400, 3424
StyI	CCWWGG	2 cut(s)	3051, 3867
TaiI	ACGT	4 cut(s)	559, 1044, 1533, 3238
TatI	WGTACW	3 cut(s)	173, 2848, 2884
TauI	GCSGC	2 cut(s)	1951, 2363
TscAI	CASTG	6 cut(s)	431, 577, 1066, 2077, 2418, 3660
TseFI	GTSAC	2 cut(s)	2072, 3951
Tsp45I	GTSAC	2 cut(s)	2072, 3951
TspGWI	ACGGA	4 cut(s)	241, 2805, 3273, 3875
TspRI	CASTG	6 cut(s)	431, 577, 1066, 2077, 2418, 3660
VneI	GTGCAC	1 cut(s)	3888
VpaK11BI	GGWCC	1 cut(s)	567
VspI	ATTAAT	2 cut(s)	1458, 2256
XbaI	TCTAGA	3 cut(s)	725, 1798, 2215
XceI	RCATGY	5 cut(s)	156, 201, 881, 1546, 3505
XhoI	CTCGAG	1 cut(s)	1071
XmiI	GTMKAC	1 cut(s)	2770
XmnI	GAANNNNTTC	3 cut(s)	449, 2435, 3075
ZrmI	AGTACT	2 cut(s)	175, 2850
Zsp2I	ATGCAT	2 cut(s)	154, 1398

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.