Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0002228

Physical Location & Seq

Genomic Coordinates Forward (+)

4411053 .. 4422191

Gene Structure 11139 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G005270.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4035 bp

ATGATCAGTCAAATCAGGGGAATGAAGCAGATATCTGCTCAGATCAGACCGAAGCCAATTTATAATATTGATGAATCTGATGATGATCCCGATTTTGGGACTGGAAAACCTGGAAGAGCTCTGGAAAAGTTTGAGAAGATTGTTAGGACTGACGTGAAAGATAATTCCTGTCAAGCCTGTGGTGAAATTGAGAATCTTATGGAATGTGAGACATGCAACTATGCATATCATGCTAAGTGTTTACTTCCCCCGTTAAAGGCTCCTCTACCTGACAGATGGCGATGTCCAGAATGTGTGAGCCCTCTAACTGATATTGACAAGATTCTGGATTGTGACAGGCGACCTACTGTGGCGGATGATAGTGATGCCACAAAGATGGGTTCAAGGCAGATTTTTGTTAAACAATATCTTGTGAAGTGGAAGGGACTATCATACTACATTGCACATGGTAACTTGATTCCTGAGAAAGAATTTTTCAAAGCATTCAAGACATACCCGCGTCTAAAGCAGAAGGTTAACAATTTCAATCGTCAAAGGTCTTCCATTAATAACACAGAGGAGGAATTTGTTGCCATTCGACCCGAATGGACAACTGTTGACCGAATCCTTGCTTGCAGGGGCGATGATGAGAAGCAATATCTTGTAAAGTGGCAAGAACTTCCTTACGATGAATGCTATTGGGAAGTGGAGGCTGATATCTCTACATTTCAGCCAGAAATAGAGAAATTCCATAGAATCCAGTCTAGATCTCACAAATTGTCTGGTAAGCAGAAGAGCAGTCTTAAAGATGCTCTCGAATCAAAAAAGAAGCAGAAAGAGTTTCAACAGTTTGAACACAGTCCTGAATTTCTTTCTGGAGGTACACTGCATCCATATCAGCTTGAAGGTTTGAACTTTTTACGCTTCTCCTGGTCCAAACAAACTCCTGTAATACTTGCAGACGAGATGGGACTTGGCAAAACTATACAGAGTATAGCTTTTTTAGCATCTCTGTTTGAAGAGAAAGTCTATCCACATCTAGTGGTGGCTCCACTTTCAACGTTACGAAATTGGGAACGGGAATTCGCAACATGGGCACCTCAAATGAATGCTGTTATGTATGTTGGCTCTTCCCAAGCCCGTGCGGTCATAAGGGAATACGAGTTTTACTTCCCCAAGAATCACAAGAATATCAAGAAAAAGAAATCTGGTCAGATCATTAGTGAGAGCAAGCAAGATAGGATCAAGTTTGATGTCCTTCTCACATCATATGAGATGATTAACTTGGACACTACATCACTGAAACCGATAAAGTGGAAGTGCATGATTGTTGATGAAGGACATCGTCTCAAAAACAAGGATTCAAAATTGTTTTCATCATTACAGCAGTTCTGGACGAATCATCGCACACTTCTGACTGGAACTCCTCTTCAGAATAATCTGGATGAACTTTTCATGCTTATGCACTTCCTTGATGCTGGCAAGTTTGCAAGTCTAGAGGAGTTCCAGGAAGAGTTTAAGGATATCAACGAGGAACAGCAGATCTCCAGGCTTCACAAAATTCTTGCTCCTCATCTTCTTAGAAGGGTGAAAAAAGACGTCATGAAAGAATTACCTCCTAAGAAGGAACTTATATTACGCATTGAATTAAGCAGCAAGCAGAAAGACTATTACAAAGCAATTCTGACTCGTAACTATCAGATACTAACTCGTCATGGTGGTGCACAGATATCTCTTATCAATGTGGTCATGGAGTTGCGCAAGCTCTGTTGTCATGCTTATATGCTCGAGGGAGTAGAACCTTACATAGAAGATGCCAATGAATCTCACAAGCAACTTTTGGAATCTTCTGGAAAACTGCAAGTATTGGACAGAATGATGGTAAAACTTAAGCAGCAAGGGCACAGGGTGCTTATCTATTCACAATTTCAACACATGTTGGATTTGCTAGAAGACTACTGTACATACAAAAAATGGCAGTATGAACGAATAGATGGTAAAGTTGGTGGAGCTGAGAGACAAATAAGGATAGATCGATTCAATGCCAAAAATTCTTCCAGGTTTTGCTTTCTGCTTTCCACTAGAGCTGGGGGCTTGGGCATAAACCTTGCAACTGCTGACACAGTGATCATATATGATAGTGATTGGAATCCTCATGCAGATCTACAAGCTATGGCTAGAGCTCATCGACTTGGGCAAACTAACAAGGTGATGATTTATAGGCTAATAACACGAGGAACAATTGAAGAAAGAATGATGCAACTGACTAAGAAGAAAATGGTATTGGAACATTTAGTTGTTGGAAGGTTAAAGGCTCGAAACATCAACCAGGAAGAGCTAGATGATATCATTCGATACGGATCAAAGGAGTTATTTGCTGATGAAAATGATGAAGCGGGAAAATCCCGTCAAATTCATTATGATGATGCTGCGATAGAGAGATTACTTGATCGTGAACAAGCTGGGGATGATGACACTCCGTTGGAGGAAGAAGATGAAGATGGATTTTTAAAGGCCTTTAAGGTTGCAAATTTTGAATACATTGACGAAGCAGAGGGCGTAGAAGAGGAGGAACCAAAAAAGGCTGCAGTTGACAGTAGTCCTACTGACAATGATAAATCAAATTTCTGGGAAGATTTGTTAAAGGACAGATATGAAGTGCATAAAGTTGAAGAATTAAACGCTTTAGGCAAAGGGAAGAGAAGTCGTAAGCAGATGGTATCTGTTGAAGACGATGATCTCGCTGGTTTGGAAGATGTAAGTTCTGATGGAGAGGATGATAACTATGAAGCTGAAGTGACGGATGGCGAAACAGCTTCCTCTGGACCTCTTTCTGGAAATCTTTCTGCAGGGAGAAAGCCTAATAAAAAGAGAAGTCGTGTGGATAGTGCAGAACCCCTTCCTTTGATGGAAGGAGAAGGAAATTCATTCAAAGTCTTGGGTTTCAATCAAAATCAGAGGGCTGCATTTCTAAAAAATTTTATGAGGTTTGGGATGGTGGATGATGACTATAAGGAGTTTGTAGCACGCATGAAGCAGAAGACATATGAAGAAATAAAAGCTTATGCGACTCTATTCTTGAAACACATAATTGAACCATTAACAGACTATCCAACCTTTTCAGATGGTGTCCCTAAAGAAGGACTAAATATAACGGACGTACTTGTTCGGTATTCAGTTATGTCTATGATTCATAAAAAAGCAAAGTTTGCAACAGAAAATCCAGGCGCTCCTTTATATGAAGATTACGTCCTGTTCCGTTACCCAGGATTGAAGGGTGGCAAATTTTGGAAGGTGGATCACGATGGGATATTACTGCATGCTGTATTAAAGCATGGCTATGGAAGATGGCAAGCTATTGTTGACGACAAGGACCTGGGGATTCAAGATGTTATTTGTCAAGAGTTGAACCTTCCTTTCCCAGGGCAGGTTAAAGCACAAAATGGAGCACATGCTAATACTGAAGCACCTGGTAACGCCGCTGGAAATGGTAATGGGAATGACGTAGGAGAAAATGTAGCTCAGGGAACCATTGATCCAGTAAACCAGCCTCAATCATATCCAGAGTCTTCTACGTCGCATTATTCAAGAGATATGCAAAGAAGATTGGTAGAATTTATCAAGCGAAGAGTGCTTCTCTTGGAGGATGGCAACAATAAAGAAGCCCAGAAAGAATACTACGATGAGTTGTATGCAAATGAGATTGCGGAGCTTGAGAAGGAAACCATGGGGACGAATTTTCCATACACTGGGGAAACTGATAAGCAAACGGGTGATGATCAGTTGCCAATAGTTGAAGAAATCGGTTCGGAAGAAATTCTAGCTGCAGCTTGTGACAATGATCCAGATCGTTTAAAATTGCCTCAGCTTTTTAATGAGATGTGCAAACTTGTGGAAGGAACTGCCCCGCAGTCTCTGCAGACTTCTTTTGAAACTATCTGCGAAGACGTACTCAGCATGACTGGCCCCCCCGTGCAACAAAATCCTCCTACTTCAGCACAGCCAACACTAAATGCAGATAAACAACCAGGAGATGAGTCAAAGAGCACAAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006338 GO:0006355 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008283 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009736 GO:0009739 GO:0009755 GO:0009787 GO:0009788 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016043 GO:0019219 GO:0019222 GO:0022622 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048364 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071368 GO:0071495 GO:0071840 GO:0080090 GO:0097159 GO:0099402 GO:1901363 GO:1901419 GO:1901420 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:1905957 GO:1905958 GO:2000023 GO:2000026 GO:2000069 GO:2000112 GO:2000113 GO:2000280 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko05165 ko05203 map05165 map05203

Pfam Domains

Protein Families

CHDCT2 Chromo DUF1086 DUF1087 Helicase_C PHD SNF2_N

Protein Analysis

1344

Amino Acids

153.88

Weight (kDa)

5.54

Isoelectric Point (pI)

44.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PHD	PF00628	57 - 98	4.9e-07	PHD-finger
Chromo	PF00385	198 - 245	1.7e-13	Chromo (CHRromatin Organisation MOdifier) domain
ResIII	PF04851	288 - 469	4.3e-09	Type III restriction enzyme, res subunit
SNF2-rel_dom	PF00176	292 - 588	4.5e-80	SNF2-related domain
HDA2-3	PF11496	541 - 754	3e-09	Class II histone deacetylase complex subunits 2 and 3
Helicase_C	PF00271	612 - 725	2.4e-24	Helicase conserved C-terminal domain
DUF1087	PF06465	848 - 906	1.3e-22	CHD subfamily II, DUF1087
CHDII_SANT-like	PF06461	943 - 1075	4.7e-51	CHD subfamily II, SANT-like domain
HTH_CHD1_Hrp3	PF23588	1090 - 1128	2.3e-06	ATP-dependent helicase CHD1-2/hrp3 HTH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002228)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25170 AT2G25170 AT2G25170 AT2G25170 AT4G31900 AT4G31900 AT4G31900
fragaria_vesca	FvH4_2g40920
malus_domestica	MD08G1058800.v1.1 MD15G1037700.v1.1
prunus_persica	Prupe.1G391600_v2.0.a1
pyrus_communis	pycom08g04860 pycom08g04870 pycom15g03490
rosa_chinensis	RchiOBHm_Chr3g0455421 RchiOBHm_Chr6g0299661
rosa_laevigata	RLG00000011399 RLG00000025364
rosa_multiflora	Rmu_co8019708.1_g000001 Rmu_co8099568.1_g000001 Rmu_sc0022216.1_g000002 Rmu_sc0024261.1_g000001 Rmu_sc0024262.1_g000001
rosa_roxburghii	Rroxscaffold_6G00424300 Rroxscaffold_7G00168470
rosa_rugosa	Rorug03G0007200 Rorug03G0007300 Rorug03G0007400 Rorug04G0013500 Rorug06G0291400
rosa_samantha	Rh3AG066700 Rh3BG069100 Rh3CG067500 Rh3DG069000 Rh5AG515500 Rh6AG402100 Rh6BG410600 Rh6CG416300 Rh6CG416400 Rh6DG403500
rosa_wichuraiana	Rw2G021110 Rw3G005270 Rw6G035200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	63
AatII	GACGTC	1 cut(s)	1581
Acc16I	TGCGCA	1 cut(s)	1739
Acc36I	ACCTGC	1 cut(s)	3397
AccB1I	GGYRCC	1 cut(s)	1075
AccB7I	CCANNNNNTGG	1 cut(s)	3728
AccII	CGCG	1 cut(s)	499
AciI	CCGC	7 cut(s)	353, 497, 1124, 2375, 3459, 3686, 3887
AclI	AACGTT	1 cut(s)	1040
AclWI	GGATC	6 cut(s)	80, 1229, 2347, 3285, 3509, 3815
AcuI	CTGAAG	4 cut(s)	1394, 2793, 3462, 3957
AcyI	GRCGYC	1 cut(s)	1578
AdeI	CACNNNGTG	1 cut(s)	1888
AfaI	GTAC	4 cut(s)	862, 1942, 3141, 3930
AfiI	CCNNNNNNNGG	8 cut(s)	95, 96, 256, 2813, 3119, 3401, 3406, 3728
AflII	CTTAAG	1 cut(s)	1868
AflIII	ACRYGT	1 cut(s)	1914
AjiI	CACGTC	1 cut(s)	154
AjuI	GAANNNNNNNTTGG	2 cut(s)	2246, 2278
AloI	GAACNNNNNNTCC	2 cut(s)	3499, 3531
Alw21I	GWGCWC	5 cut(s)	121, 1705, 2164, 3430, 4028
Alw26I	GTCTC	4 cut(s)	203, 1331, 1990, 3897
Alw44I	GTGCAC	1 cut(s)	1701
AlwI	GGATC	6 cut(s)	80, 1229, 2347, 3285, 3509, 3815
AlwNI	CAGNNNCTG	1 cut(s)	3895
Ama87I	CYCGRG	1 cut(s)	1766
AoxI	GGCC	2 cut(s)	2493, 3943
ApaLI	GTGCAC	1 cut(s)	1701
ApeKI	GCWGC	7 cut(s)	1632, 1873, 2408, 2564, 2942, 3803, 3806
AseI	ATTAAT	1 cut(s)	546
Asp700I	GAANNNNTTC	3 cut(s)	2821, 2877, 3795
AspLEI	GCGC	2 cut(s)	1740, 3209
AspS9I	GGNCC	4 cut(s)	912, 2804, 3352, 3944
AsuHPI	GGTGA	4 cut(s)	194, 1579, 2200, 3764
AvaI	CYCGRG	1 cut(s)	1766
AvaII	GGWCC	3 cut(s)	912, 2804, 3352
BaeGI	GKGCMC	3 cut(s)	1078, 1705, 1884
BanI	GGYRCC	1 cut(s)	1075
BanII	GRGCYC	3 cut(s)	121, 302, 2164
BauI	CACGAG	1 cut(s)	2211
BbsI	GAAGAC	6 cut(s)	531, 1938, 2715, 3026, 3540, 3930
Bbv12I	GWGCWC	5 cut(s)	121, 1705, 2164, 3430, 4028
BbvCI	CCTCAGC	1 cut(s)	3843
BbvI	GCAGC	7 cut(s)	1644, 1885, 2395, 2551, 2929, 3790, 3818
BclI	TGATCA	3 cut(s)	3, 2106, 3757
BcoDI	GTCTC	4 cut(s)	203, 1331, 1990, 3897
BfaI	CTAG	8 cut(s)	744, 1019, 1475, 1928, 2061, 2157, 2318, 3800
BfmI	CTRYAG	4 cut(s)	2565, 2826, 3804, 3896
BfoI	RGCGCY	1 cut(s)	3210
BfrI	CTTAAG	1 cut(s)	1868
BfuAI	ACCTGC	1 cut(s)	3397
BglII	AGATCT	3 cut(s)	746, 1521, 2140
BisI	GCNGC	8 cut(s)	1633, 1874, 2409, 2565, 2943, 3459, 3804, 3807
BlsI	GCNGC	8 cut(s)	1634, 1875, 2410, 2566, 2944, 3460, 3805, 3808
Bme18I	GGWCC	3 cut(s)	912, 2804, 3352
BmeT110I	CYCGRG	1 cut(s)	1766
BmgBI	CACGTC	1 cut(s)	154
BmgT120I	GGNCC	4 cut(s)	912, 2804, 3352, 3944
BmiI	GGNNCC	6 cut(s)	261, 1029, 1077, 2553, 3508, 3946
BmrI	ACTGGG	1 cut(s)	3738
BmsI	GCATC	8 cut(s)	355, 778, 877, 995, 1444, 1783, 2226, 2395
BmuI	ACTGGG	1 cut(s)	3738
BoxI	GACNNNNGTC	1 cut(s)	2577
BpiI	GAAGAC	6 cut(s)	531, 1938, 2715, 3026, 3540, 3930
BplI	GAGNNNNNCTC	2 cut(s)	3600, 3632
BpmI	CTGGAG	2 cut(s)	876, 1510
Bpu10I	CCTNAGC	2 cut(s)	3501, 3843
BpuEI	CTTGAG	1 cut(s)	3713
Bsa29I	ATCGAT	1 cut(s)	2014
BsaBI	GATNNNNATC	4 cut(s)	84, 2127, 2338, 3825
BsaHI	GRCGYC	1 cut(s)	1578
BsaJI	CCNNGG	5 cut(s)	3244, 3355, 3400, 3401, 3705
BsaXI	ACNNNNNCTCC	4 cut(s)	1387, 1417, 3480, 3510
Bsc4I	CCNNNNNNNGG	8 cut(s)	95, 96, 256, 2813, 3119, 3401, 3406, 3728
Bse1I	ACTGG	6 cut(s)	106, 739, 1402, 3518, 3733, 3946
Bse3DI	GCAATG	1 cut(s)	438
Bse8I	GATNNNNATC	4 cut(s)	84, 2127, 2338, 3825
BseCI	ATCGAT	1 cut(s)	2014
BseDI	CCNNGG	5 cut(s)	3244, 3355, 3400, 3401, 3705
BseGI	GGATG	9 cut(s)	361, 868, 1429, 2452, 2761, 2788, 2979, 2986, 3631
BseJI	GATNNNNATC	4 cut(s)	84, 2127, 2338, 3825
BseLI	CCNNNNNNNGG	8 cut(s)	95, 96, 256, 2813, 3119, 3401, 3406, 3728
BseMI	GCAATG	1 cut(s)	438
BseMII	CTCAG	6 cut(s)	53, 453, 1983, 3515, 3857, 3946
BseNI	ACTGG	6 cut(s)	106, 739, 1402, 3518, 3733, 3946
BseRI	GAGGAG	6 cut(s)	252, 572, 1395, 1493, 1539, 2561
BseSI	GKGCMC	3 cut(s)	1078, 1705, 1884
BseXI	GCAGC	7 cut(s)	1644, 1885, 2395, 2551, 2929, 3790, 3818
BseYI	CCCAGC	2 cut(s)	2066, 2441
BsgI	GTGCAG	1 cut(s)	2889
Bsh1236I	CGCG	1 cut(s)	499
BshFI	GGCC	2 cut(s)	2495, 3945
BshNI	GGYRCC	1 cut(s)	1075
BshVI	ATCGAT	1 cut(s)	2014
BsiHKAI	GWGCWC	5 cut(s)	121, 1705, 2164, 3430, 4028
BsiHKCI	CYCGRG	1 cut(s)	1766
BslFI	GGGAC	5 cut(s)	112, 438, 963, 3095, 3724
BslI	CCNNNNNNNGG	8 cut(s)	95, 96, 256, 2813, 3119, 3401, 3406, 3728
BsmAI	GTCTC	4 cut(s)	203, 1331, 1990, 3897
BsmBI	CGTCTC	1 cut(s)	1331
BsmFI	GGGAC	5 cut(s)	112, 438, 963, 3095, 3724
BsmI	GAATGC	3 cut(s)	482, 677, 1093
BsnI	GGCC	2 cut(s)	2495, 3945
BsoBI	CYCGRG	1 cut(s)	1766
Bsp1286I	GDGCHC	8 cut(s)	121, 302, 1078, 1705, 1884, 2164, 3430, 4028
Bsp1407I	TGTACA	1 cut(s)	1940
Bsp19I	CCATGG	1 cut(s)	3705
BspACI	CCGC	7 cut(s)	353, 497, 1124, 2375, 3459, 3686, 3887
BspANI	GGCC	2 cut(s)	2495, 3945
BspCNI	CTCAG	6 cut(s)	52, 454, 1984, 3514, 3856, 3945
BspDI	ATCGAT	1 cut(s)	2014
BspFNI	CGCG	1 cut(s)	499
BspHI	TCATGA	1 cut(s)	1581
BspLI	GGNNCC	6 cut(s)	261, 1029, 1077, 2553, 3508, 3946
BspMAI	CTGCAG	4 cut(s)	2569, 2830, 3808, 3900
BspMI	ACCTGC	1 cut(s)	3397
BspPI	GGATC	6 cut(s)	80, 1229, 2347, 3285, 3509, 3815
BspQI	GCTCTTC	4 cut(s)	109, 767, 1114, 2307
BspT107I	GGYRCC	1 cut(s)	1075
BspTI	CTTAAG	1 cut(s)	1868
BsrDI	GCAATG	1 cut(s)	438
BsrGI	TGTACA	1 cut(s)	1940
BsrI	ACTGG	6 cut(s)	106, 739, 1402, 3518, 3733, 3946
BssECI	CCNNGG	5 cut(s)	3244, 3355, 3400, 3401, 3705
BssNI	GRCGYC	1 cut(s)	1578
BssSI	CACGAG	1 cut(s)	2211
BssT1I	CCWWGG	1 cut(s)	3705
Bst2BI	CACGAG	1 cut(s)	2211
Bst4CI	ACNGT	7 cut(s)	349, 595, 828, 839, 1940, 2104, 2576
BstACI	GRCGYC	1 cut(s)	1578
BstAFI	CTTAAG	1 cut(s)	1868
BstAPI	GCANNNNNTGC	4 cut(s)	230, 1888, 3188, 3895
BstAUI	TGTACA	1 cut(s)	1940
BstC8I	GCNNGC	8 cut(s)	613, 1211, 1459, 1637, 1742, 3007, 3300, 3333
BstDSI	CCRYGG	1 cut(s)	3705
BstENI	CCTNNNNNAGG	2 cut(s)	3117, 3399
BstF5I	GGATG	9 cut(s)	361, 868, 1429, 2452, 2761, 2788, 2979, 2986, 3631
BstFNI	CGCG	1 cut(s)	499
BstH2I	RGCGCY	1 cut(s)	3210
BstHHI	GCGC	2 cut(s)	1740, 3209
BstMAI	GTCTC	4 cut(s)	203, 1331, 1990, 3897
BstNSI	RCATGY	4 cut(s)	216, 1918, 3302, 3434
BstPAI	GACNNNNGTC	1 cut(s)	2577
BstSFI	CTRYAG	4 cut(s)	2565, 2826, 3804, 3896
BstSLI	GKGCMC	3 cut(s)	1078, 1705, 1884
BstUI	CGCG	1 cut(s)	499
BstV1I	GCAGC	7 cut(s)	1644, 1885, 2395, 2551, 2929, 3790, 3818
BstV2I	GAAGAC	6 cut(s)	531, 1938, 2715, 3026, 3540, 3930
BstX2I	RGATCY	3 cut(s)	746, 1521, 2140
BstXI	CCANNNNNNTGG	1 cut(s)	376
BstYI	RGATCY	3 cut(s)	746, 1521, 2140
Bsu15I	ATCGAT	1 cut(s)	2014
BsuRI	GGCC	2 cut(s)	2495, 3945
BsuTUI	ATCGAT	1 cut(s)	2014
BtgI	CCRYGG	1 cut(s)	3705
BtgZI	GCGATG	3 cut(s)	295, 636, 1367
BtrI	CACGTC	1 cut(s)	154
BtsCI	GGATG	9 cut(s)	361, 868, 1429, 2452, 2761, 2788, 2979, 2986, 3631
BtsI	GCAGTG	1 cut(s)	863
BtsIMutI	CAGTG	4 cut(s)	863, 1277, 2109, 3726
BveI	ACCTGC	1 cut(s)	3397
Cac8I	GCNNGC	8 cut(s)	613, 1211, 1459, 1637, 1742, 3007, 3300, 3333
CaiI	CAGNNNCTG	1 cut(s)	3895
CciI	TCATGA	1 cut(s)	1581
CfoI	GCGC	2 cut(s)	1740, 3209
Cfr13I	GGNCC	4 cut(s)	912, 2804, 3352, 3944
ClaI	ATCGAT	1 cut(s)	2014
CseI	GACGC	1 cut(s)	488
CsiI	ACCWGGT	1 cut(s)	3448
Csp6I	GTAC	4 cut(s)	861, 1941, 3140, 3929
CviQI	GTAC	4 cut(s)	861, 1941, 3140, 3929
DraI	TTTAAA	2 cut(s)	2490, 3834
DraIII	CACNNNGTG	1 cut(s)	1888
EciI	GGCGGA	1 cut(s)	368
Ecl136II	GAGCTC	2 cut(s)	119, 2162
Eco130I	CCWWGG	1 cut(s)	3705
Eco147I	AGGCCT	1 cut(s)	2495
Eco24I	GRGCYC	3 cut(s)	121, 302, 2164
Eco32I	GATATC	5 cut(s)	33, 697, 1504, 1710, 2326
Eco47I	GGWCC	3 cut(s)	912, 2804, 3352
Eco53kI	GAGCTC	2 cut(s)	119, 2162
Eco57I	CTGAAG	4 cut(s)	1394, 2793, 3462, 3957
Eco88I	CYCGRG	1 cut(s)	1766
EcoICRI	GAGCTC	2 cut(s)	119, 2162
EcoNI	CCTNNNNNAGG	2 cut(s)	3117, 3399
EcoO109I	RGGNCCY	1 cut(s)	3352
EcoRI	GAATTC	1 cut(s)	1061
EcoRV	GATATC	5 cut(s)	33, 697, 1504, 1710, 2326
EcoT14I	CCWWGG	1 cut(s)	3705
EcoT22I	ATGCAT	1 cut(s)	226
EcoT38I	GRGCYC	3 cut(s)	121, 302, 2164
ErhI	CCWWGG	1 cut(s)	3705
Esp3I	CGTCTC	1 cut(s)	1331
FaqI	GGGAC	5 cut(s)	112, 438, 963, 3095, 3724
FauI	CCCGC	3 cut(s)	504, 2368, 3894
FauNDI	CATATG	2 cut(s)	1249, 3025
FbaI	TGATCA	3 cut(s)	3, 2106, 3757
Fnu4HI	GCNGC	8 cut(s)	1633, 1874, 2409, 2565, 2943, 3459, 3804, 3807
FokI	GGATG	9 cut(s)	368, 855, 1436, 2459, 2768, 2795, 2986, 2993, 3638
FriOI	GRGCYC	3 cut(s)	121, 302, 2164
Fsp4HI	GCNGC	8 cut(s)	1633, 1874, 2409, 2565, 2943, 3459, 3804, 3807
FspBI	CTAG	8 cut(s)	744, 1019, 1475, 1928, 2061, 2157, 2318, 3800
FspI	TGCGCA	1 cut(s)	1739
GlaI	GCGC	2 cut(s)	1739, 3208
GluI	GCNGC	8 cut(s)	1633, 1874, 2409, 2565, 2943, 3459, 3804, 3807
GsaI	CCCAGC	2 cut(s)	2070, 2445
GsuI	CTGGAG	2 cut(s)	876, 1510
HaeII	RGCGCY	1 cut(s)	3210
HaeIII	GGCC	2 cut(s)	2495, 3945
HgaI	GACGC	1 cut(s)	488
HhaI	GCGC	2 cut(s)	1740, 3209
Hin1I	GRCGYC	1 cut(s)	1578
Hin6I	GCGC	2 cut(s)	1738, 3207
HinP1I	GCGC	2 cut(s)	1738, 3207
HincII	GTYRAC	4 cut(s)	517, 598, 2572, 3343
HindII	GTYRAC	4 cut(s)	517, 598, 2572, 3343
HindIII	AAGCTT	1 cut(s)	3039
HpaI	GTTAAC	1 cut(s)	517
HphI	GGTGA	4 cut(s)	194, 1579, 2200, 3764
Hpy166II	GTNNAC	9 cut(s)	242, 517, 598, 863, 1703, 2435, 2572, 3343, 3523
Hpy8I	GTNNAC	9 cut(s)	242, 517, 598, 863, 1703, 2435, 2572, 3343, 3523
Hpy99I	CGWCG	1 cut(s)	3559
HpyCH4III	ACNGT	7 cut(s)	349, 595, 828, 839, 1940, 2104, 2576
HpyCH4IV	ACGT	8 cut(s)	153, 1040, 1578, 3138, 3228, 3483, 3554, 3927
HpySE526I	ACGT	8 cut(s)	153, 1040, 1578, 3138, 3228, 3483, 3554, 3927
Hsp92I	GRCGYC	1 cut(s)	1578
HspAI	GCGC	2 cut(s)	1738, 3207
Ksp22I	TGATCA	3 cut(s)	3, 2106, 3757
KspAI	GTTAAC	1 cut(s)	517
LguI	GCTCTTC	4 cut(s)	109, 767, 1114, 2307
LmnI	GCTCC	7 cut(s)	265, 1033, 1552, 1988, 3214, 3425, 3688
Lsp1109I	GCAGC	7 cut(s)	1644, 1885, 2395, 2551, 2929, 3790, 3818
LweI	GCATC	8 cut(s)	355, 778, 877, 995, 1444, 1783, 2226, 2395
MabI	ACCWGGT	1 cut(s)	3448
MaeI	CTAG	8 cut(s)	744, 1019, 1475, 1928, 2061, 2157, 2318, 3800
MaeII	ACGT	8 cut(s)	153, 1040, 1578, 3138, 3228, 3483, 3554, 3927
MaeIII	GTNAC	8 cut(s)	332, 449, 1041, 1670, 2776, 3239, 3452, 3812
MfeI	CAATTG	1 cut(s)	2220
MflI	RGATCY	3 cut(s)	746, 1521, 2140
MhlI	GDGCHC	8 cut(s)	121, 302, 1078, 1705, 1884, 2164, 3430, 4028
MlyI	GAGTC	4 cut(s)	1660, 3043, 3554, 4025
MmeI	TCCRAC	4 cut(s)	1899, 2260, 2442, 3116
Mph1103I	ATGCAT	1 cut(s)	226
MroXI	GAANNNNTTC	3 cut(s)	2821, 2877, 3795
MslI	CAYNNNNRTG	5 cut(s)	374, 1439, 1698, 2400, 3318
MspA1I	CMGCKG	1 cut(s)	3461
MspCI	CTTAAG	1 cut(s)	1868
MunI	CAATTG	1 cut(s)	2220
Mva1269I	GAATGC	3 cut(s)	482, 677, 1093
MvnI	CGCG	1 cut(s)	499
NcoI	CCATGG	1 cut(s)	3705
NdeI	CATATG	2 cut(s)	1249, 3025
NlaIV	GGNNCC	6 cut(s)	261, 1029, 1077, 2553, 3508, 3946
NmuCI	GTSAC	3 cut(s)	332, 2776, 3812
NsbI	TGCGCA	1 cut(s)	1739
NsiI	ATGCAT	1 cut(s)	226
NspI	RCATGY	4 cut(s)	216, 1918, 3302, 3434
PaeI	GCATGC	1 cut(s)	3302
PaeR7I	CTCGAG	1 cut(s)	1766
PagI	TCATGA	1 cut(s)	1581
PasI	CCCWGGG	1 cut(s)	3401
PceI	AGGCCT	1 cut(s)	2495
PciI	ACATGT	1 cut(s)	1914
PciSI	GCTCTTC	4 cut(s)	109, 767, 1114, 2307
PctI	GAATGC	3 cut(s)	482, 677, 1093
PdmI	GAANNNNTTC	3 cut(s)	2821, 2877, 3795
PflFI	GACNNNGTC	1 cut(s)	1323
PflMI	CCANNNNNTGG	1 cut(s)	3728
PfoI	TCCNGGA	1 cut(s)	1485
PkrI	GCNGC	8 cut(s)	1634, 1875, 2410, 2566, 2944, 3460, 3805, 3808
PleI	GAGTC	4 cut(s)	1660, 3043, 3553, 4024
PpsI	GAGTC	4 cut(s)	1660, 3043, 3553, 4024
PpuMI	RGGWCCY	1 cut(s)	3352
PscI	ACATGT	1 cut(s)	1914
PshAI	GACNNNNGTC	1 cut(s)	2577
PshBI	ATTAAT	1 cut(s)	546
PsiI	TTATAA	1 cut(s)	63
Psp124BI	GAGCTC	2 cut(s)	121, 2164
Psp1406I	AACGTT	1 cut(s)	1040
Psp5II	RGGWCCY	1 cut(s)	3352
PspFI	CCCAGC	2 cut(s)	2066, 2441
PspN4I	GGNNCC	6 cut(s)	261, 1029, 1077, 2553, 3508, 3946
PspPI	GGNCC	4 cut(s)	912, 2804, 3352, 3944
PspPPI	RGGWCCY	1 cut(s)	3352
PspXI	VCTCGAGB	1 cut(s)	1766
PsrI	GAACNNNNNNTAC	2 cut(s)	1599, 1631
PstI	CTGCAG	4 cut(s)	2569, 2830, 3808, 3900
PstNI	CAGNNNCTG	1 cut(s)	3895
PsuI	RGATCY	3 cut(s)	746, 1521, 2140
PsyI	GACNNNGTC	1 cut(s)	1323
RsaI	GTAC	4 cut(s)	862, 1942, 3141, 3930
RsaNI	GTAC	4 cut(s)	861, 1941, 3140, 3929
RseI	CAYNNNNRTG	5 cut(s)	374, 1439, 1698, 2400, 3318
SacI	GAGCTC	2 cut(s)	121, 2164
SapI	GCTCTTC	4 cut(s)	109, 767, 1114, 2307
SatI	GCNGC	8 cut(s)	1633, 1874, 2409, 2565, 2943, 3459, 3804, 3807
Sau96I	GGNCC	4 cut(s)	912, 2804, 3352, 3944
SchI	GAGTC	4 cut(s)	1660, 3043, 3554, 4025
SduI	GDGCHC	8 cut(s)	121, 302, 1078, 1705, 1884, 2164, 3430, 4028
SexAI	ACCWGGT	1 cut(s)	3448
SfaNI	GCATC	8 cut(s)	355, 778, 877, 995, 1444, 1783, 2226, 2395
SfcI	CTRYAG	4 cut(s)	2565, 2826, 3804, 3896
Sfr274I	CTCGAG	1 cut(s)	1766
SinI	GGWCC	3 cut(s)	912, 2804, 3352
SlaI	CTCGAG	1 cut(s)	1766
SmiMI	CAYNNNNRTG	5 cut(s)	374, 1439, 1698, 2400, 3318
SmlI	CTYRAG	3 cut(s)	1766, 1868, 3692
SmoI	CTYRAG	3 cut(s)	1766, 1868, 3692
SphI	GCATGC	1 cut(s)	3302
SseBI	AGGCCT	1 cut(s)	2495
SsiI	CCGC	7 cut(s)	353, 497, 1124, 2375, 3459, 3686, 3887
SspI	AATATT	1 cut(s)	67
SspMI	CTAG	8 cut(s)	744, 1019, 1475, 1928, 2061, 2157, 2318, 3800
SstI	GAGCTC	2 cut(s)	121, 2164
StuI	AGGCCT	1 cut(s)	2495
StyI	CCWWGG	1 cut(s)	3705
TaaI	ACNGT	7 cut(s)	349, 595, 828, 839, 1940, 2104, 2576
TaiI	ACGT	8 cut(s)	156, 1043, 1581, 3141, 3231, 3486, 3557, 3930
TaqI	TCGA	7 cut(s)	577, 795, 1767, 2014, 2167, 2296, 2332
TaqII	GACCGA	2 cut(s)	64, 615
TatI	WGTACW	1 cut(s)	1940
TauI	GCSGC	1 cut(s)	3461
TscAI	CASTG	4 cut(s)	870, 1284, 2109, 3733
TseFI	GTSAC	3 cut(s)	332, 2776, 3812
TseI	GCWGC	7 cut(s)	1632, 1873, 2408, 2564, 2942, 3803, 3806
Tsp45I	GTSAC	3 cut(s)	332, 2776, 3812
TspGWI	ACGGA	5 cut(s)	2352, 2448, 2795, 3149, 3227
TspRI	CASTG	4 cut(s)	870, 1284, 2109, 3733
Tth111I	GACNNNGTC	1 cut(s)	1323
Van91I	CCANNNNNTGG	1 cut(s)	3728
Vha464I	CTTAAG	1 cut(s)	1868
VneI	GTGCAC	1 cut(s)	1701
VpaK11BI	GGWCC	3 cut(s)	912, 2804, 3352
VspI	ATTAAT	1 cut(s)	546
XagI	CCTNNNNNAGG	2 cut(s)	3117, 3399
XbaI	TCTAGA	2 cut(s)	743, 1474
XceI	RCATGY	4 cut(s)	216, 1918, 3302, 3434
XhoI	CTCGAG	1 cut(s)	1766
XmnI	GAANNNNTTC	3 cut(s)	2821, 2877, 3795
XspI	CTAG	8 cut(s)	744, 1019, 1475, 1928, 2061, 2157, 2318, 3800
ZraI	GACGTC	1 cut(s)	1579
Zsp2I	ATGCAT	1 cut(s)	226

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.