Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0002228

Physical Location & Seq

Genomic Coordinates Forward (+)

4670929 .. 4681801

Gene Structure 10873 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG069000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3957 bp

ATGAGTAGCTTGGTTGAGAGGCTGCGCACTAGATCAGACCGAAAGCCAATTTATAATATTGATGAATCTGATGATGATCCTGATTTTGGGACTGGAAAACCTGGAAGAGCTCAGGAAAAGTTTGAGAAGATTGTTAGGACTGACGTGAAAGATAATTCCTGTCAAGCCTGTGGTGAAATTGAGAATCTTATGGAATGTGAGACATGCAACTATGCATATCATGCTAAGTGTTTACTTCCCCCGTTAAAGGCTCCTCTACCTGACAGATGGCGATGTCCAGAATGTGTGAGCCCTTTAACTGATATTGACAAGATTCTGGATTGTGACAGGCGACCTACTGTGGCAGATGATAGTGATGCCACAAAGATGGGTTCAAGGCAGATTTTTGTTAAACAATATCTTGTGAAGTGGAAGGGACTATCATATCTACATTGCACATGGATTCCTGAGAAAGAATTTTTCAAAGCATTCAAGACATACCCGCGTCTAAAGCAGAAGGTTAACAATTTCAATCGTCAAAGGTCTTCCATTAATAACACAGAGGAGGAATTTGTTGCCATTCGACCCGAATGGACAACTGTTGACCGGATCCTTGCTTGCAGGGGCGATAATGAGAAGCAATATCTTGTAAAGTGGCAAGAACTTCCTTACGATGAATGCTATTGGGAAGTGGAGGCTGATATCTTTACATTTCAGCCAGAAATAGAGAAATTCCATAGAATCCAGTCTAGATCTCACAAATTGTCTGGTAAGCAGAAGAGCAGTCTTAAAGATGCTCTCGAATCAAAAAAGAAGCAGAAAGAGTTTCAACAGTTTGAACACAGTCCTGAATTTCTTTCTGGAGGTACACTGCATCCATATCAGCTTGAAGGTTTGAACTTTTTACGCTTCTCCTGGTCCAAACAAACTCCTGTAATACTTGCAGACGAGATGGGACTTGGCAAAACTATACAGAGTATAGCTTTTTTAGCATCTCTGTTTGAAGAGAAAGTCTATCCACATCTAGTGGTGGCTCCACTTTCAACATTACGAAATTGGGAACGGGAATTCGCAACATGGGCACCTCAAATGAATGTTGTTATGTATGTTGGCTCTTCCCAAGCCCGTGCGGTCATAAGGGAATACGAGTTTTACTTCCCCAAGAATCACAAGAATATCAAGAAAAAGAAATCTGGTCAGATCATTAGTGAGAGCAAGCAAGATAGGATCAAGTTTGATGTCCTTCTCACATCATATGAGATGATTAACTTGGACTCTACATCACTGAAACCAATAAAGTGGAAGTGCATGATTGTTGATGAAGGACATCGTCTCAAAAACAAGGATTCAAAATTGTTTTCATCATTACAGCAGTTCTGGACGAATCATCGCACACTTCTGACTGGAACTCCTCTTCAGAATAATCTGGATGAACTTTTCATGCTTATGCACTTCCTTGATGCTGGCAAGGAAAAAATTGCTGAAATGGTAATTGGCACTGCAGGGGTGAAAAAAGATGTCATGAAAGAATTACCTCCTAAGAAGGAACTTATATTACGCATTGAATTAAGCAGCAAGCAGAAAGACTATTACAAAGCAATTCTGACTCGTAACTATCAGATACTAACTCGTCATGGTGGTGCACAGATATCTCTTATCAATGTGGTCATGGAGTTGCGCAAGCTCTGTTGTCATGCTTATATGCTCGAGGGGGTCGAACCTTACATAGAAGATGCCAATGAATCTCACAAGCAACTTTTGGAATCTTCTGGAAAACTGCAAGTATTGGACAGAATGATGGTAAAACTTAAGCAGCAAGGGCACAGGGTGCTTATCTATTCACAATTTCAACACATGTTGGATTTGCTAGAAGACTACTGTACATACAAAAAATGGCAGTATGAACGAATAGATGGTAAAGTTGGTGGAGCTGAGAGACAAATAAGGATAGATCGATTCAATGCCAAAAATTCTTCCAGGTTTTGCTTTCTGCTTTCCACTAGAGCTGGGGGCTTGGGCATAAACCTTGCAACTGCTGACACAGTGATCATATATGATAGTGATTGGAATCCTCATGCAGATCTACAAGCTATGGCTAGAGCTCATCGACTTGGGCAAACTAACAAGGTGATGATTTATAGGCTAATAACACGAGGAACAATTGAAGAAAGAATGATGCAACTGACTAAGAAGAAAATGGTATTGGAACATTTGGTTGTTGGAAGGTTAAAGGCTCGAAACATCAACCAGGAAGAGCTAGATGACATTATTCGATACGGATCAAAGGAGTTATTTGCTGGTGAAAATGATGAAGAGGGAAAATCCCGTCAAATTCATTATGATGATGCTGCGATAGAGAGATTACTTGATCGTGAACAAGCTGGGGATGATGACACTCCGTTGGAGGAAGAAGATGAAGATGGATTTTTAAAGGCCTTTAAGGTTGCAAATTTTGAATACATTGACGAAGCAGAGGGCGTAGAAGAGGAGGAACCAAAAAAGGCTGCAGTTGACAGTAGTCCTACTGACAATGATAAATCAAATTTCTGGGAAGATTTGTTAAAGGACAGATATGAAGTGCATAAAGTTGAAGAATTAAACGCTTTAGGCAAAGGGAAGAGAAGTCGTAAGCAGATGGTATCTGTTGAAGACGATGATCTCGCTGGTTTGGAAGATGTAAGTTCTGATGGAGAGGATGATAACTATGAAGCTGAAGTGACGGATGGCGAAACAGCTTCCTCTGGACCTCTTTCTGGAAATCTTTCTGCAGGGAGAAAGCCTAATAAAAAGAGAAGTCGTGTGGATGGTGCAGAACCCCTTCCTTTGATGGAAGGAGAAGGAAATTCATTCAAAGTCTTGGGTTTCAATCAAAATCAGAGGGCTGCATTTCTAAAATTTTTTATGAGGTTTGGAATGGTGGATGATGACTATAAGGAGTTTGTAGCACGCATGAAGCAGAAGACATATGAAGAAATAAAAGCTTATGCCACTCTATTCTTGAAACACATAATTGAACCATTAACAGACTCCCCAACCTTTTCAGATGGTGTCCCTAAAGAAGGACTCAATATAACGGATGTACTTGTTCGGTATTCAGTTATGTCTATGATTCATAAAAAAGCAAAGTTTGCAACAGAAAATCCAGGCGCACCTTTATATGAAGATTACATCCTGTTCCGTTACCCAGGATTGAAGGGTGGCAAATTTTGGAAGGTGGATCACGATGGGATATTACTGCACGCTGTATTAAAGCATGGCTATGGAAGATGGCAAGCTATTGTTGACGACAAGGACCTGGGGATTCAAGAAGTTATTTGCCAAGAGTTGAATCTTCCTGTCCCAGGGCAGGTTAAAGCACAAAATGGAGCACATGCTAATACTGAAGCACCTGGTAACGCCGCTGGAAATGGTAATGGGAATGACGTAGGAGAAAATGTAGCTCAGGGAACCATTGATCCAGTAAACCAGCCTCAATCATATCCAGAGTCTTCTACGTCGCATTATTCAAGAGATATGCAAAGAAGATTGGTAGAATTCATCAAGCGAAGAGTGCTTCTCTTGGAGGATGGCAACAATAAAGAAGCCCAGAAAGAATACTATGATGAGCTGTATGCAAATGAGATTGCGGAGCTTGAGAAAGAAACCATGGGGACGAATTTTCCATACGCTGGGGAAACTGATAAGCAAATGGGTGATGATCAGTTGCCAATAGTTGAAGAAATCGGTTCGGAAGAAATTCTAGCTGCAGCTTGTGACGACGATCCAGATCGTTTAAAATTGCCTCAGCTTTTTAATGAGATGTGCAAACTTGTGGAAGGAACTGCCCCGCAGTCTCTGCAGACTTCTTTTGAAACTATCTGCGAAGACGTACTAAGCATGACTGGCCCCCCAGTGCAACAAAATCCTCCTACTTCAGCACAGCCAACACTAAATATTGCAGATAAACAACCAGGAGATGAGTCAAAGAGCACAAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006338 GO:0006355 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008283 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009736 GO:0009739 GO:0009755 GO:0009787 GO:0009788 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016043 GO:0019219 GO:0019222 GO:0022622 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048364 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071368 GO:0071495 GO:0071840 GO:0080090 GO:0097159 GO:0099402 GO:1901363 GO:1901419 GO:1901420 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:1905957 GO:1905958 GO:2000023 GO:2000026 GO:2000069 GO:2000112 GO:2000113 GO:2000280 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko05165 ko05203 map05165 map05203

Pfam Domains

Protein Families

CHDCT2 Chromo DUF1086 DUF1087 Helicase_C PHD SNF2_N

Protein Analysis

1318

Amino Acids

150.74

Weight (kDa)

5.52

Isoelectric Point (pI)

45.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PHD	PF00628	54 - 95	4.8e-07	PHD-finger
Chromo	PF00385	123 - 155	3.9e-08	Chromo (CHRromatin Organisation MOdifier) domain
Chromo	PF00385	193 - 240	1.8e-12	Chromo (CHRromatin Organisation MOdifier) domain
ResIII	PF04851	283 - 464	3.9e-09	Type III restriction enzyme, res subunit
SNF2-rel_dom	PF00176	287 - 482	1.7e-53	SNF2-related domain
SNF2-rel_dom	PF00176	496 - 561	1.3e-09	SNF2-related domain
HDA2-3	PF11496	514 - 727	2.8e-09	Class II histone deacetylase complex subunits 2 and 3
Helicase_C	PF00271	585 - 698	2.3e-24	Helicase conserved C-terminal domain
DUF1087	PF06465	821 - 879	1.2e-22	CHD subfamily II, DUF1087
CHDII_SANT-like	PF06461	916 - 1048	1.2e-51	CHD subfamily II, SANT-like domain
HTH_CHD1_Hrp3	PF23588	1063 - 1101	6.4e-06	ATP-dependent helicase CHD1-2/hrp3 HTH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002228)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25170 AT2G25170 AT2G25170 AT2G25170 AT4G31900 AT4G31900 AT4G31900
fragaria_vesca	FvH4_2g40920
malus_domestica	MD08G1058800.v1.1 MD15G1037700.v1.1
prunus_persica	Prupe.1G391600_v2.0.a1
pyrus_communis	pycom08g04860 pycom08g04870 pycom15g03490
rosa_chinensis	RchiOBHm_Chr3g0455421 RchiOBHm_Chr6g0299661
rosa_laevigata	RLG00000011399 RLG00000025364
rosa_multiflora	Rmu_co8019708.1_g000001 Rmu_co8099568.1_g000001 Rmu_sc0022216.1_g000002 Rmu_sc0024261.1_g000001 Rmu_sc0024262.1_g000001
rosa_roxburghii	Rroxscaffold_6G00424300 Rroxscaffold_7G00168470
rosa_rugosa	Rorug03G0007200 Rorug03G0007300 Rorug03G0007400 Rorug04G0013500 Rorug06G0291400
rosa_samantha	Rh3AG066700 Rh3BG069100 Rh3CG067500 Rh3DG069000 Rh5AG515500 Rh6AG402100 Rh6BG410600 Rh6CG416300 Rh6CG416400 Rh6DG403500
rosa_wichuraiana	Rw2G021110 Rw3G005270 Rw6G035200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	54
Acc16I	TGCGCA	2 cut(s)	26, 1658
Acc36I	ACCTGC	1 cut(s)	3316
AccB1I	GGYRCC	1 cut(s)	1060
AccB7I	CCANNNNNTGG	1 cut(s)	3647
AccII	CGCG	1 cut(s)	484
AciI	CCGC	5 cut(s)	482, 1109, 3378, 3605, 3806
AclWI	GGATC	8 cut(s)	71, 583, 596, 1214, 2266, 3204, 3428, 3734
AcuI	CTGAAG	4 cut(s)	1379, 2712, 3381, 3876
AdeI	CACNNNGTG	1 cut(s)	1807
AfaI	GTAC	4 cut(s)	847, 1861, 3060, 3849
AfiI	CCNNNNNNNGG	7 cut(s)	86, 247, 2732, 3038, 3320, 3325, 3647
AflII	CTTAAG	1 cut(s)	1787
AflIII	ACRYGT	1 cut(s)	1833
AjiI	CACGTC	1 cut(s)	145
AjuI	GAANNNNNNNTTGG	4 cut(s)	1455, 1487, 2165, 2197
AloI	GAACNNNNNNTCC	2 cut(s)	3418, 3450
Alw21I	GWGCWC	5 cut(s)	112, 1624, 2083, 3349, 3950
Alw26I	GTCTC	4 cut(s)	194, 1316, 1909, 3816
Alw44I	GTGCAC	1 cut(s)	1620
AlwI	GGATC	8 cut(s)	71, 583, 596, 1214, 2266, 3204, 3428, 3734
AlwNI	CAGNNNCTG	1 cut(s)	3814
Ama87I	CYCGRG	1 cut(s)	1685
AoxI	GGCC	2 cut(s)	2412, 3862
ApaLI	GTGCAC	1 cut(s)	1620
ApeKI	GCWGC	8 cut(s)	22, 1551, 1792, 2327, 2483, 2861, 3722, 3725
AseI	ATTAAT	1 cut(s)	531
Asp700I	GAANNNNTTC	3 cut(s)	2740, 2796, 3714
AspLEI	GCGC	3 cut(s)	27, 1659, 3128
AspS9I	GGNCC	4 cut(s)	897, 2723, 3271, 3863
AsuHPI	GGTGA	5 cut(s)	185, 1498, 2119, 2291, 3683
AvaI	CYCGRG	1 cut(s)	1685
AvaII	GGWCC	3 cut(s)	897, 2723, 3271
BaeGI	GKGCMC	3 cut(s)	1063, 1624, 1803
BamHI	GGATCC	1 cut(s)	588
BanI	GGYRCC	1 cut(s)	1060
BanII	GRGCYC	3 cut(s)	112, 293, 2083
BauI	CACGAG	1 cut(s)	2130
BbsI	GAAGAC	6 cut(s)	516, 1857, 2634, 2945, 3459, 3849
Bbv12I	GWGCWC	5 cut(s)	112, 1624, 2083, 3349, 3950
BbvCI	CCTCAGC	1 cut(s)	3762
BbvI	GCAGC	8 cut(s)	9, 1563, 1804, 2314, 2470, 2848, 3709, 3737
BclI	TGATCA	2 cut(s)	2025, 3676
BcoDI	GTCTC	4 cut(s)	194, 1316, 1909, 3816
BfaI	CTAG	8 cut(s)	30, 729, 1004, 1847, 1980, 2076, 2237, 3719
BfmI	CTRYAG	5 cut(s)	1479, 2484, 2745, 3723, 3815
BfrI	CTTAAG	1 cut(s)	1787
BfuAI	ACCTGC	1 cut(s)	3316
BglII	AGATCT	2 cut(s)	731, 2059
BisI	GCNGC	9 cut(s)	23, 1552, 1793, 2328, 2484, 2862, 3378, 3723, 3726
BlsI	GCNGC	9 cut(s)	24, 1553, 1794, 2329, 2485, 2863, 3379, 3724, 3727
Bme18I	GGWCC	3 cut(s)	897, 2723, 3271
BmeT110I	CYCGRG	1 cut(s)	1685
BmgBI	CACGTC	1 cut(s)	145
BmgT120I	GGNCC	4 cut(s)	897, 2723, 3271, 3863
BmiI	GGNNCC	7 cut(s)	252, 590, 1014, 1062, 2472, 3427, 3865
BmrI	ACTGGG	1 cut(s)	3863
BmsI	GCATC	8 cut(s)	346, 763, 862, 980, 1429, 1702, 2145, 2314
BmuI	ACTGGG	1 cut(s)	3863
BoxI	GACNNNNGTC	1 cut(s)	2496
BpiI	GAAGAC	6 cut(s)	516, 1857, 2634, 2945, 3459, 3849
BplI	GAGNNNNNCTC	2 cut(s)	3519, 3551
BpmI	CTGGAG	1 cut(s)	861
Bpu10I	CCTNAGC	3 cut(s)	111, 3420, 3762
BpuEI	CTTGAG	1 cut(s)	3632
Bsa29I	ATCGAT	1 cut(s)	1933
BsaBI	GATNNNNATC	4 cut(s)	75, 2046, 2257, 3744
BsaJI	CCNNGG	5 cut(s)	3163, 3274, 3319, 3320, 3624
BsaWI	WCCGGW	1 cut(s)	585
BsaXI	ACNNNNNCTCC	4 cut(s)	1372, 1402, 3399, 3429
Bsc4I	CCNNNNNNNGG	7 cut(s)	86, 247, 2732, 3038, 3320, 3325, 3647
Bse1I	ACTGG	6 cut(s)	97, 724, 1387, 3437, 3865, 3869
Bse3DI	GCAATG	1 cut(s)	430
Bse8I	GATNNNNATC	4 cut(s)	75, 2046, 2257, 3744
BseCI	ATCGAT	1 cut(s)	1933
BseDI	CCNNGG	5 cut(s)	3163, 3274, 3319, 3320, 3624
BseJI	GATNNNNATC	4 cut(s)	75, 2046, 2257, 3744
BseLI	CCNNNNNNNGG	7 cut(s)	86, 247, 2732, 3038, 3320, 3325, 3647
BseMI	GCAATG	1 cut(s)	430
BseMII	CTCAG	5 cut(s)	125, 438, 1902, 3434, 3776
BseNI	ACTGG	6 cut(s)	97, 724, 1387, 3437, 3865, 3869
BseRI	GAGGAG	4 cut(s)	243, 557, 1380, 2480
BseSI	GKGCMC	3 cut(s)	1063, 1624, 1803
BseXI	GCAGC	8 cut(s)	9, 1563, 1804, 2314, 2470, 2848, 3709, 3737
BseYI	CCCAGC	3 cut(s)	1985, 2360, 3647
BsgI	GTGCAG	2 cut(s)	2808, 3200
Bsh1236I	CGCG	1 cut(s)	484
BshFI	GGCC	2 cut(s)	2414, 3864
BshNI	GGYRCC	1 cut(s)	1060
BshVI	ATCGAT	1 cut(s)	1933
BsiHKAI	GWGCWC	5 cut(s)	112, 1624, 2083, 3349, 3950
BsiHKCI	CYCGRG	1 cut(s)	1685
BsiSI	CCGG	1 cut(s)	586
BslFI	GGGAC	6 cut(s)	103, 429, 948, 3014, 3302, 3643
BslI	CCNNNNNNNGG	7 cut(s)	86, 247, 2732, 3038, 3320, 3325, 3647
BsmAI	GTCTC	4 cut(s)	194, 1316, 1909, 3816
BsmBI	CGTCTC	1 cut(s)	1316
BsmFI	GGGAC	6 cut(s)	103, 429, 948, 3014, 3302, 3643
BsmI	GAATGC	2 cut(s)	467, 662
BsnI	GGCC	2 cut(s)	2414, 3864
BsoBI	CYCGRG	1 cut(s)	1685
Bsp1286I	GDGCHC	8 cut(s)	112, 293, 1063, 1624, 1803, 2083, 3349, 3950
Bsp1407I	TGTACA	1 cut(s)	1859
Bsp19I	CCATGG	1 cut(s)	3624
BspACI	CCGC	5 cut(s)	482, 1109, 3378, 3605, 3806
BspANI	GGCC	2 cut(s)	2414, 3864
BspCNI	CTCAG	5 cut(s)	124, 439, 1903, 3433, 3775
BspDI	ATCGAT	1 cut(s)	1933
BspFNI	CGCG	1 cut(s)	484
BspHI	TCATGA	1 cut(s)	1500
BspLI	GGNNCC	7 cut(s)	252, 590, 1014, 1062, 2472, 3427, 3865
BspMAI	CTGCAG	5 cut(s)	1483, 2488, 2749, 3727, 3819
BspMI	ACCTGC	1 cut(s)	3316
BspPI	GGATC	8 cut(s)	71, 583, 596, 1214, 2266, 3204, 3428, 3734
BspQI	GCTCTTC	4 cut(s)	100, 752, 1099, 2226
BspT107I	GGYRCC	1 cut(s)	1060
BspTI	CTTAAG	1 cut(s)	1787
BsrDI	GCAATG	1 cut(s)	430
BsrGI	TGTACA	1 cut(s)	1859
BsrI	ACTGG	6 cut(s)	97, 724, 1387, 3437, 3865, 3869
BssECI	CCNNGG	5 cut(s)	3163, 3274, 3319, 3320, 3624
BssSI	CACGAG	1 cut(s)	2130
BssT1I	CCWWGG	1 cut(s)	3624
Bst2BI	CACGAG	1 cut(s)	2130
Bst4CI	ACNGT	7 cut(s)	340, 580, 813, 824, 1859, 2023, 2495
BstAFI	CTTAAG	1 cut(s)	1787
BstAPI	GCANNNNNTGC	4 cut(s)	221, 1807, 3107, 3814
BstAUI	TGTACA	1 cut(s)	1859
BstC8I	GCNNGC	8 cut(s)	598, 1196, 1444, 1556, 1661, 2926, 3219, 3252
BstDEI	CTNAG	9 cut(s)	111, 225, 447, 1518, 1911, 2166, 3420, 3762, 3851
BstDSI	CCRYGG	1 cut(s)	3624
BstENI	CCTNNNNNAGG	2 cut(s)	3036, 3318
BstFNI	CGCG	1 cut(s)	484
BstHHI	GCGC	3 cut(s)	27, 1659, 3128
BstMAI	GTCTC	4 cut(s)	194, 1316, 1909, 3816
BstNSI	RCATGY	3 cut(s)	207, 1837, 3353
BstPAI	GACNNNNGTC	1 cut(s)	2496
BstSFI	CTRYAG	5 cut(s)	1479, 2484, 2745, 3723, 3815
BstSLI	GKGCMC	3 cut(s)	1063, 1624, 1803
BstUI	CGCG	1 cut(s)	484
BstV1I	GCAGC	8 cut(s)	9, 1563, 1804, 2314, 2470, 2848, 3709, 3737
BstV2I	GAAGAC	6 cut(s)	516, 1857, 2634, 2945, 3459, 3849
BstX2I	RGATCY	3 cut(s)	588, 731, 2059
BstXI	CCANNNNNNTGG	2 cut(s)	367, 1278
BstYI	RGATCY	3 cut(s)	588, 731, 2059
Bsu15I	ATCGAT	1 cut(s)	1933
BsuRI	GGCC	2 cut(s)	2414, 3864
BsuTUI	ATCGAT	1 cut(s)	1933
BtgI	CCRYGG	1 cut(s)	3624
BtgZI	GCGATG	2 cut(s)	286, 1352
BtrI	CACGTC	1 cut(s)	145
BtsI	GCAGTG	2 cut(s)	848, 1476
BtsIMutI	CAGTG	5 cut(s)	848, 1262, 1476, 2028, 3876
BveI	ACCTGC	1 cut(s)	3316
Cac8I	GCNNGC	8 cut(s)	598, 1196, 1444, 1556, 1661, 2926, 3219, 3252
CaiI	CAGNNNCTG	1 cut(s)	3814
CciI	TCATGA	1 cut(s)	1500
CfoI	GCGC	3 cut(s)	27, 1659, 3128
Cfr13I	GGNCC	4 cut(s)	897, 2723, 3271, 3863
ClaI	ATCGAT	1 cut(s)	1933
CseI	GACGC	1 cut(s)	473
CsiI	ACCWGGT	1 cut(s)	3367
Csp6I	GTAC	4 cut(s)	846, 1860, 3059, 3848
CviQI	GTAC	4 cut(s)	846, 1860, 3059, 3848
DdeI	CTNAG	9 cut(s)	111, 225, 447, 1518, 1911, 2166, 3420, 3762, 3851
DraI	TTTAAA	2 cut(s)	2409, 3753
DraIII	CACNNNGTG	1 cut(s)	1807
Ecl136II	GAGCTC	2 cut(s)	110, 2081
Eco130I	CCWWGG	1 cut(s)	3624
Eco147I	AGGCCT	1 cut(s)	2414
Eco24I	GRGCYC	3 cut(s)	112, 293, 2083
Eco32I	GATATC	2 cut(s)	682, 1629
Eco47I	GGWCC	3 cut(s)	897, 2723, 3271
Eco53kI	GAGCTC	2 cut(s)	110, 2081
Eco57I	CTGAAG	4 cut(s)	1379, 2712, 3381, 3876
Eco88I	CYCGRG	1 cut(s)	1685
EcoICRI	GAGCTC	2 cut(s)	110, 2081
EcoNI	CCTNNNNNAGG	2 cut(s)	3036, 3318
EcoO109I	RGGNCCY	1 cut(s)	3271
EcoRI	GAATTC	2 cut(s)	1046, 3512
EcoRV	GATATC	2 cut(s)	682, 1629
EcoT14I	CCWWGG	1 cut(s)	3624
EcoT22I	ATGCAT	1 cut(s)	217
EcoT38I	GRGCYC	3 cut(s)	112, 293, 2083
ErhI	CCWWGG	1 cut(s)	3624
Esp3I	CGTCTC	1 cut(s)	1316
FaqI	GGGAC	6 cut(s)	103, 429, 948, 3014, 3302, 3643
FauI	CCCGC	2 cut(s)	489, 3813
FauNDI	CATATG	2 cut(s)	1234, 2944
FbaI	TGATCA	2 cut(s)	2025, 3676
Fnu4HI	GCNGC	9 cut(s)	23, 1552, 1793, 2328, 2484, 2862, 3378, 3723, 3726
FriOI	GRGCYC	3 cut(s)	112, 293, 2083
Fsp4HI	GCNGC	9 cut(s)	23, 1552, 1793, 2328, 2484, 2862, 3378, 3723, 3726
FspBI	CTAG	8 cut(s)	30, 729, 1004, 1847, 1980, 2076, 2237, 3719
FspI	TGCGCA	2 cut(s)	26, 1658
GlaI	GCGC	3 cut(s)	26, 1658, 3127
GluI	GCNGC	9 cut(s)	23, 1552, 1793, 2328, 2484, 2862, 3378, 3723, 3726
GsaI	CCCAGC	3 cut(s)	1989, 2364, 3651
GsuI	CTGGAG	1 cut(s)	861
HaeIII	GGCC	2 cut(s)	2414, 3864
HapII	CCGG	1 cut(s)	586
HgaI	GACGC	1 cut(s)	473
HhaI	GCGC	3 cut(s)	27, 1659, 3128
Hin6I	GCGC	3 cut(s)	25, 1657, 3126
HinP1I	GCGC	3 cut(s)	25, 1657, 3126
HincII	GTYRAC	4 cut(s)	502, 583, 2491, 3262
HindII	GTYRAC	4 cut(s)	502, 583, 2491, 3262
HindIII	AAGCTT	1 cut(s)	2958
HpaI	GTTAAC	1 cut(s)	502
HpaII	CCGG	1 cut(s)	586
HphI	GGTGA	5 cut(s)	185, 1498, 2119, 2291, 3683
Hpy166II	GTNNAC	9 cut(s)	233, 502, 583, 848, 1622, 2354, 2491, 3262, 3442
Hpy8I	GTNNAC	9 cut(s)	233, 502, 583, 848, 1622, 2354, 2491, 3262, 3442
Hpy99I	CGWCG	2 cut(s)	3478, 3740
HpyCH4III	ACNGT	7 cut(s)	340, 580, 813, 824, 1859, 2023, 2495
HpyCH4IV	ACGT	4 cut(s)	144, 3402, 3473, 3846
HpyF3I	CTNAG	9 cut(s)	111, 225, 447, 1518, 1911, 2166, 3420, 3762, 3851
HpySE526I	ACGT	4 cut(s)	144, 3402, 3473, 3846
HspAI	GCGC	3 cut(s)	25, 1657, 3126
Ksp22I	TGATCA	2 cut(s)	2025, 3676
KspAI	GTTAAC	1 cut(s)	502
LguI	GCTCTTC	4 cut(s)	100, 752, 1099, 2226
LmnI	GCTCC	5 cut(s)	256, 1018, 1907, 3344, 3607
Lsp1109I	GCAGC	8 cut(s)	9, 1563, 1804, 2314, 2470, 2848, 3709, 3737
LweI	GCATC	8 cut(s)	346, 763, 862, 980, 1429, 1702, 2145, 2314
MabI	ACCWGGT	1 cut(s)	3367
MaeI	CTAG	8 cut(s)	30, 729, 1004, 1847, 1980, 2076, 2237, 3719
MaeII	ACGT	4 cut(s)	144, 3402, 3473, 3846
MaeIII	GTNAC	6 cut(s)	323, 1589, 2695, 3158, 3371, 3731
MfeI	CAATTG	1 cut(s)	2139
MflI	RGATCY	3 cut(s)	588, 731, 2059
MhlI	GDGCHC	8 cut(s)	112, 293, 1063, 1624, 1803, 2083, 3349, 3950
MlyI	GAGTC	6 cut(s)	1247, 1579, 2999, 3036, 3473, 3947
MmeI	TCCRAC	3 cut(s)	1818, 2179, 2361
Mph1103I	ATGCAT	1 cut(s)	217
MroXI	GAANNNNTTC	3 cut(s)	2740, 2796, 3714
MslI	CAYNNNNRTG	5 cut(s)	365, 1424, 1617, 2319, 3237
MspA1I	CMGCKG	1 cut(s)	3380
MspCI	CTTAAG	1 cut(s)	1787
MspI	CCGG	1 cut(s)	586
MunI	CAATTG	1 cut(s)	2139
Mva1269I	GAATGC	2 cut(s)	467, 662
MvnI	CGCG	1 cut(s)	484
NcoI	CCATGG	1 cut(s)	3624
NdeI	CATATG	2 cut(s)	1234, 2944
NlaIV	GGNNCC	7 cut(s)	252, 590, 1014, 1062, 2472, 3427, 3865
NmuCI	GTSAC	3 cut(s)	323, 2695, 3731
NsbI	TGCGCA	2 cut(s)	26, 1658
NsiI	ATGCAT	1 cut(s)	217
NspI	RCATGY	3 cut(s)	207, 1837, 3353
PaeR7I	CTCGAG	1 cut(s)	1685
PagI	TCATGA	1 cut(s)	1500
PasI	CCCWGGG	1 cut(s)	3320
PceI	AGGCCT	1 cut(s)	2414
PciI	ACATGT	1 cut(s)	1833
PciSI	GCTCTTC	4 cut(s)	100, 752, 1099, 2226
PcsI	WCGNNNNNNNCGW	1 cut(s)	1692
PctI	GAATGC	2 cut(s)	467, 662
PdmI	GAANNNNTTC	3 cut(s)	2740, 2796, 3714
PflFI	GACNNNGTC	1 cut(s)	1308
PflMI	CCANNNNNTGG	1 cut(s)	3647
PkrI	GCNGC	9 cut(s)	24, 1553, 1794, 2329, 2485, 2863, 3379, 3724, 3727
PleI	GAGTC	6 cut(s)	1247, 1579, 2999, 3036, 3472, 3946
PpsI	GAGTC	6 cut(s)	1247, 1579, 2999, 3036, 3472, 3946
PpuMI	RGGWCCY	1 cut(s)	3271
PscI	ACATGT	1 cut(s)	1833
PshAI	GACNNNNGTC	1 cut(s)	2496
PshBI	ATTAAT	1 cut(s)	531
PsiI	TTATAA	1 cut(s)	54
Psp124BI	GAGCTC	2 cut(s)	112, 2083
Psp5II	RGGWCCY	1 cut(s)	3271
PspFI	CCCAGC	3 cut(s)	1985, 2360, 3647
PspN4I	GGNNCC	7 cut(s)	252, 590, 1014, 1062, 2472, 3427, 3865
PspPI	GGNCC	4 cut(s)	897, 2723, 3271, 3863
PspPPI	RGGWCCY	1 cut(s)	3271
PspXI	VCTCGAGB	1 cut(s)	1685
PsrI	GAACNNNNNNTAC	2 cut(s)	1518, 1550
PstI	CTGCAG	5 cut(s)	1483, 2488, 2749, 3727, 3819
PstNI	CAGNNNCTG	1 cut(s)	3814
PsuI	RGATCY	3 cut(s)	588, 731, 2059
PsyI	GACNNNGTC	1 cut(s)	1308
RsaI	GTAC	4 cut(s)	847, 1861, 3060, 3849
RsaNI	GTAC	4 cut(s)	846, 1860, 3059, 3848
RseI	CAYNNNNRTG	5 cut(s)	365, 1424, 1617, 2319, 3237
SacI	GAGCTC	2 cut(s)	112, 2083
SapI	GCTCTTC	4 cut(s)	100, 752, 1099, 2226
SatI	GCNGC	9 cut(s)	23, 1552, 1793, 2328, 2484, 2862, 3378, 3723, 3726
Sau96I	GGNCC	4 cut(s)	897, 2723, 3271, 3863
SchI	GAGTC	6 cut(s)	1247, 1579, 2999, 3036, 3473, 3947
SduI	GDGCHC	8 cut(s)	112, 293, 1063, 1624, 1803, 2083, 3349, 3950
SexAI	ACCWGGT	1 cut(s)	3367
SfaNI	GCATC	8 cut(s)	346, 763, 862, 980, 1429, 1702, 2145, 2314
SfcI	CTRYAG	5 cut(s)	1479, 2484, 2745, 3723, 3815
Sfr274I	CTCGAG	1 cut(s)	1685
SinI	GGWCC	3 cut(s)	897, 2723, 3271
SlaI	CTCGAG	1 cut(s)	1685
SmiMI	CAYNNNNRTG	5 cut(s)	365, 1424, 1617, 2319, 3237
SmlI	CTYRAG	3 cut(s)	1685, 1787, 3611
SmoI	CTYRAG	3 cut(s)	1685, 1787, 3611
SseBI	AGGCCT	1 cut(s)	2414
SsiI	CCGC	5 cut(s)	482, 1109, 3378, 3605, 3806
SspI	AATATT	2 cut(s)	58, 3913
SspMI	CTAG	8 cut(s)	30, 729, 1004, 1847, 1980, 2076, 2237, 3719
SstI	GAGCTC	2 cut(s)	112, 2083
StuI	AGGCCT	1 cut(s)	2414
StyI	CCWWGG	1 cut(s)	3624
TaaI	ACNGT	7 cut(s)	340, 580, 813, 824, 1859, 2023, 2495
TaiI	ACGT	4 cut(s)	147, 3405, 3476, 3849
TaqI	TCGA	8 cut(s)	562, 780, 1686, 1695, 1933, 2086, 2215, 2251
TaqII	GACCGA	1 cut(s)	54
TatI	WGTACW	2 cut(s)	1859, 3058
TauI	GCSGC	1 cut(s)	3380
TscAI	CASTG	5 cut(s)	855, 1269, 1483, 2028, 3876
TseFI	GTSAC	3 cut(s)	323, 2695, 3731
TseI	GCWGC	8 cut(s)	22, 1551, 1792, 2327, 2483, 2861, 3722, 3725
Tsp45I	GTSAC	3 cut(s)	323, 2695, 3731
TspGWI	ACGGA	5 cut(s)	2271, 2367, 2714, 3068, 3146
TspRI	CASTG	5 cut(s)	855, 1269, 1483, 2028, 3876
Tth111I	GACNNNGTC	1 cut(s)	1308
Van91I	CCANNNNNTGG	1 cut(s)	3647
Vha464I	CTTAAG	1 cut(s)	1787
VneI	GTGCAC	1 cut(s)	1620
VpaK11BI	GGWCC	3 cut(s)	897, 2723, 3271
VspI	ATTAAT	1 cut(s)	531
XagI	CCTNNNNNAGG	2 cut(s)	3036, 3318
XbaI	TCTAGA	1 cut(s)	728
XceI	RCATGY	3 cut(s)	207, 1837, 3353
XhoI	CTCGAG	1 cut(s)	1685
XmnI	GAANNNNTTC	3 cut(s)	2740, 2796, 3714
XspI	CTAG	8 cut(s)	30, 729, 1004, 1847, 1980, 2076, 2237, 3719
Zsp2I	ATGCAT	1 cut(s)	217

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.