Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002228

Physical Location & Seq

Genomic Coordinates Forward (+)

4674223 .. 4689791

Gene Structure 15569 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1058800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4212 bp

ATGAGTCAGATGATGAGGCTGATTTTGTATCTGGAAAACGTGGGACAGCTCCGGAAAAGTTTGAAAAGATTATTAGGAGTGACGCGGTTTAAAGAGAATTCATGTCAAGCCTGTGGCGGAAGTGGGAATCTTCTGGACTGTGAAACATGTAGTTATGCTTACCATTCTAAGTGTTTACTTCCACCATTGAGATCGCCTCCCCCTGGCAACTGGAGATGCCCTGAATGTGTAAGCCCGTTAAATGATATTGATAAGATTTTGGACTGTGAAATGCGCCCTACTGTAGCTGGTGATAGTGATGCCTCAACGCTGGGCTCAAAGCAAATTTTTGTAAAACAATATCTTGTAAAGTGGAAGGGACTATCTTATCTGCATTGCACATGGGTTCCAGAGAAGGAGTTTGTGAAAGCTTTTAAGGCCCATCCACGTTTGAAGACAAAAGTAAATAATTTCCATCGTCAAATGGAATCAAGTAATAACTCTGAGGATGATTTTGTTGCCATTCGACCTGAATGGACTACTGTTGATCGAATTCTGGCTTGCAGGGAAGACGATGAGAAAGAATATTTAGTAAAGTGGAAAGAACTTCCCTATGATGAGTGCTATTGGGAATCCGAATCGGACATAGCTGCATTTCAGCCGGAAATAGGGAGATTCAACAGAATTCAGTCTAGGTCTCATAAAGTTTTGTCTAGTAAGCAGAAGAGCAGTCTTAGAGATGCCATGGACTCAAAATATAAGCAGAAAGAGTTTCAACAGTATGAAGGCAGTCCCGACTTTCTTTCTGGAGGCACTCTGCATCCGTATCAGCTTGAAGGGTTGAACTTCTTACGTTTCTCCTGGTCCAAACAAACTCATGTTATACTTGCAGATGAGATGGGACTTGGTAAAACCATACAGAGTATTGCTTTTTTAGCATCTCTTTTTGAAGAGAAAGTCTCTCCACATCTAGTTGTGGCTCCACTTTCAACATTAAGAAATTGGGAACGGGAATTTGCAACATGGGCACCTCAAATGAATGTGGTTATGTATGTTGGCTCTTCCCAAGCGCGTGCTGTCATACGGGAATATGAATTTTACTTCCCCAAAAATCACAAGAAGATAAAGAAAAAGAAATCTGGTCAAATTGTTAGCGAAAGCAAGCAAGATAGGATCAAATTTGATGTTCTCTTGACATCGTATGAGATGATCAACTTAGACTCAACGTCACTAAAACCAATAAAGTGGGAGTGCATGATTGTTGATGAAGGTCATCGTCTAAAAAATAAGGATTCAAAATTATTCTCATCATTACAGCAGTATTGGACTAATCATCGTGTGCTTCTGACCGGCACTCCCCTCCAGAACAATCTGGACGAGCTCTTTATGCTTATGCATTTCCTTGATGCTGGGAAGTTCGGAAGTCTGGAAGAGTTTCAGGAAGAGTTTAAGGATATAAATCAAGAGGAACAAGTTTCACGGCTTCATAGAATGTTGGCTCCCCATCTCCTAAGAAGAGTGAAAAAGGATGTGATGAAGGAACTACCTCCTAAAAAGGAACTGATATTACGTGTTGATTTAAGCAGCAAACAGAAAGAGTATTACAAAGCAATTCTGACCCGTAACTATCAGATACTAACTCGTCGAGGTGGTGCACAGATATCTCTTATCAATGTGGTTATGGAATTGCGCAAGCTTTGTTGTCATCCGTTTATGTTAGAGGGAGTTGAACCAGACATAGAAGATCCAAATGAAGCTTTCAAACAGCTATTGGAAACTTCTGGGAAATTGCAATTGTTAGACAAAATGATGGTGAAACTTAAGGAGCAAGGGCATAGAGTTCTCATATATTCACAGTTTCAGCACATGCTTGATTTGCTAGAAGACTATTGTACTTTCAAAGTATTAATTCGTCGCTATTTTGTTAAATGGCAGTACGAACGGATAGATGGAAAAGTTGGTGGAGCTGAGAGACAAATAAGAATAGATCGATTTAATGCCAAAAATTCTTCTAGATTTTGTTTTCTGCTATCTACTCGAGCTGGGGGCTTAGGAATAAACCTTGCAACTGCAGATACGGTGATCATATATGATAGTGATTGGAATCCTCATGCTGATCTACAAGCTATGGCAAGAGCTCATCGACTTGGGCAAACTAACAAGGTAATGATTTATAGGCTAGTAACCCGAGGAAGCATTGAAGAACGGATGATGGAAATGACAAAGAAGAAAATGGTATTGGAGCATTTGGTTGTTGGAAGGTTAAAGAAACAAGATATCAACCAGGAAGAGTTAGATGACATCATAAGGTATGGATCGAAGGAGTTATTTGTTGACGAAAATGATGAAGCAGGAAAATCCCGTCAAATCCATTATGATGACGCTGCAATAGATAGACTACTGGATCGTGATCAAGCTGGGGATGATGAGGCTTTGTTGGATGATGAAGATGAAGATGGATTTTTAAAGGCCTTCAAGGTTGCGAATTTTGAATATATTGATGAAGCAGAGGCTGTAGCAGAGGAGGAACCACAAAAGGTTACCATGGAAAGTCGACCTCTTGCGAGCAGTTATGAAAGAACAAATTACTGGGAAGAGTTATTAAAAGACAAATATGAAGTGCATAAAGTTGAAGAATTTAATGCTTTGGGAAAAGGAAAGCGAAGTCGTAAGCAGATGGTATCTGTTGATGATGACGATCTTGCTGGTTTGGAAGATGTGAGTTCTGACGGTGAAGATGATAACTATGAAGCTGAAGTGATGGATGGTGAAACATCATCATCCGGAACTGTTTCTGGGGGGAAGAGGCTGCCTAACAGAAAGAGGAGCCGTGTGGAAAGTATGGAGCCCCCTCCCTTGATGGAAGGAGAAGGAAGATCCTTCAAAGTTTTGGGTTTCAATCAAAGTCAGAGAGCCATGTTTGTACAAGTCTTGATGAGGTTCGGAGTGGGGGATTATGACTGGAAAGAGTTTACAGCACGCATGAAGAAGACATTTGAAGAAGTAGATAGATATGCTAAACTATTCTTGGAGCACATAGCTGAAGGAGATAATGACTCTCCAACCTTTGCAGATGGTGTACCTAAAGACGGGCTCAGAATAGGAGATGTACTTATTAGGGTTGCAACTTTGATGCTGGTGAAGAAAAGGGTGGAAGCAGCATTAAGAAATCCAGGGGCTCCACTTTTTTCACAGGATACCTTGTTGTTCCACCCTGGATTGAAGGGTGGAAAGTATTGGAAGGAGGAACATGATTTCACATTGCTGCGTGCCGTAATTAAGCATGGATATGGAAGATGGCAAGCTATTGTTGATGACAAGGACCTGAGGCTTCAAGAAGTTATCTGTCAAGAGTTGAATCTCCCCTTTATAAATTTACCCCTCCCTGGACAAGTTAATACACAAGCACAGAATGGTGAGAAAGCAACAAATGGAGAAGGACCTAGCAACCATGCTAGTGAAAATGGTAGCGGAAGTGACATAGGAGCTAATGTTTCCCAGGGAACCAGTGATGCCGTGAACCAACCTCAGTTGTTTCATAACTCTTCTGTGTTATTTCAATATAGAGATATGCAGAGAAGGCAGGTAGAATTTATTAAGAAGAGGGTGCTTCTTTTGGAGAAAGGGAACGTTTCAGAGTTGATTGCAGAGGAATATGGTGAAGCAACTGTGGTTGGAAGTGAAGAACCTGAGAGTGCACCGAATGCTACAATATTGTCAAGCCCTCATTCCGTGGAGACTAATGGTCACATGGTTGATCAGTTGCCTAGAATAGTAGACATCACTCCCGAAGAAGTTGCTGCTGCAGCTTGTGACAACGATCCAGATCGATTGAAATTGCCTCGCCTTTATAATGAGATGTGCAACGTCGTGGAGCGAAATGCCCATATTTCACTTGAGACTTCTTTAGGAACCATTTGTGAAGCGATAAACATGACTCTCTCTTCTGTGCCCCATCATCCTCCACCAGAATCTCTCATATTGAATCCAGGTAAGCAATCGGAAGCTGAGTCAAGCGGCGGTGTTTTTCTGAAACCACCATCTCCTCAAGGTGACGGAAAGCATGCCGTCGTGGCAGACTCTGAGATGACAAATTTGGCTGCAGAACCTGAACCTACAATCATGGAGCTAGACCCTGAACCCGTGGACGGGATAATCTGTGATGATCGGGGAAAGCAAGAACAGAACAATGTCGATTCTAAGGGAAAGGGGGTCATTGTATTGGATGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006338 GO:0006355 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008283 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009736 GO:0009739 GO:0009755 GO:0009787 GO:0009788 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016043 GO:0019219 GO:0019222 GO:0022622 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048364 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071368 GO:0071495 GO:0071840 GO:0080090 GO:0097159 GO:0099402 GO:1901363 GO:1901419 GO:1901420 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:1905957 GO:1905958 GO:2000023 GO:2000026 GO:2000069 GO:2000112 GO:2000113 GO:2000280 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko05165 ko05203 map05165 map05203

Pfam Domains

Protein Families

CHDCT2 Chromo DUF1086 DUF1087 Helicase_C PHD SNF2_N

Protein Analysis

1404

Amino Acids

159.8

Weight (kDa)

5.6

Isoelectric Point (pI)

42.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PHD	PF00628	35 - 76	5e-08	PHD-finger
Chromo	PF00385	96 - 137	1.4e-08	Chromo (CHRromatin Organisation MOdifier) domain
Chromo	PF00385	174 - 220	9.9e-14	Chromo (CHRromatin Organisation MOdifier) domain
ResIII	PF04851	265 - 446	1.8e-10	Type III restriction enzyme, res subunit
SNF2-rel_dom	PF00176	269 - 564	9.6e-83	SNF2-related domain
HDA2-3	PF11496	516 - 739	1.2e-07	Class II histone deacetylase complex subunits 2 and 3
Helicase_C	PF00271	589 - 710	1.9e-23	Helicase conserved C-terminal domain
DUF1087	PF06465	833 - 893	2e-24	CHD subfamily II, DUF1087
CHDII_SANT-like	PF06461	929 - 1058	4.7e-48	CHD subfamily II, SANT-like domain
CHDCT2	PF08074	1072 - 1102	5.3e-06	CHDCT2 (NUC038) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0002228)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25170 AT2G25170 AT2G25170 AT2G25170 AT4G31900 AT4G31900 AT4G31900
fragaria_vesca	FvH4_2g40920
malus_domestica	MD08G1058800.v1.1 MD15G1037700.v1.1
prunus_persica	Prupe.1G391600_v2.0.a1
pyrus_communis	pycom08g04860 pycom08g04870 pycom15g03490
rosa_chinensis	RchiOBHm_Chr3g0455421 RchiOBHm_Chr6g0299661
rosa_laevigata	RLG00000011399 RLG00000025364
rosa_multiflora	Rmu_co8019708.1_g000001 Rmu_co8099568.1_g000001 Rmu_sc0022216.1_g000002 Rmu_sc0024261.1_g000001 Rmu_sc0024262.1_g000001
rosa_roxburghii	Rroxscaffold_6G00424300 Rroxscaffold_7G00168470
rosa_rugosa	Rorug03G0007200 Rorug03G0007300 Rorug03G0007400 Rorug04G0013500 Rorug06G0291400
rosa_samantha	Rh3AG066700 Rh3BG069100 Rh3CG067500 Rh3DG069000 Rh5AG515500 Rh6AG402100 Rh6BG410600 Rh6CG416300 Rh6CG416400 Rh6DG403500
rosa_wichuraiana	Rw2G021110 Rw3G005270 Rw6G035200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	3350, 3831
Acc16I	TGCGCA	1 cut(s)	1670
Acc36I	ACCTGC	1 cut(s)	3553
AccB1I	GGYRCC	1 cut(s)	1006
AccI	GTMKAC	2 cut(s)	2533, 3756
AccII	CGCG	2 cut(s)	85, 1051
AccIII	TCCGGA	2 cut(s)	51, 2762
AciI	CCGC	5 cut(s)	85, 117, 3450, 3996, 3999
AclI	AACGTT	1 cut(s)	3609
AclWI	GGATC	6 cut(s)	1160, 1718, 2302, 2391, 2850, 3794
AcuI	CTGAAG	2 cut(s)	2754, 3042
AfaI	GTAC	5 cut(s)	1873, 1916, 2904, 3060, 3090
AfiI	CCNNNNNNNGG	6 cut(s)	203, 647, 3068, 3365, 4127, 4128
AflII	CTTAAG	1 cut(s)	1799
AflIII	ACRYGT	2 cut(s)	146, 1549
AhdI	GACNNNNNGTC	2 cut(s)	1204, 3723
AjnI	CCWGG	8 cut(s)	202, 839, 2262, 3151, 3193, 3364, 3477, 3967
AjuI	GAANNNNNNNTTGG	2 cut(s)	2201, 2233
Alw21I	GWGCWC	5 cut(s)	1362, 1636, 2119, 3015, 3679
Alw26I	GTCTC	5 cut(s)	681, 943, 1945, 3710, 3872
Alw44I	GTGCAC	2 cut(s)	1632, 3675
AlwI	GGATC	6 cut(s)	1160, 1718, 2302, 2391, 2850, 3794
AlwNI	CAGNNNCTG	3 cut(s)	2492, 4061, 4088
Ama87I	CYCGRG	2 cut(s)	2016, 2166
Aor13HI	TCCGGA	2 cut(s)	51, 2762
AoxI	GGCC	2 cut(s)	417, 2448
ApaLI	GTGCAC	2 cut(s)	1632, 3675
AseI	ATTAAT	1 cut(s)	1886
Asp700I	GAANNNNTTC	2 cut(s)	1413, 2770
AspLEI	GCGC	3 cut(s)	276, 1051, 1671
AspS9I	GGNCC	4 cut(s)	418, 843, 3301, 3419
AsuHPI	GGTGA	9 cut(s)	302, 1804, 2071, 2723, 2759, 3130, 3407, 3650, 4043
AvaI	CYCGRG	2 cut(s)	2016, 2166
AvaII	GGWCC	3 cut(s)	843, 3301, 3419
AxyI	CCTNAGG	1 cut(s)	3305
BaeGI	GKGCMC	4 cut(s)	1009, 1636, 3679, 3933
BanI	GGYRCC	1 cut(s)	1006
BanII	GRGCYC	6 cut(s)	317, 1362, 2119, 2829, 3075, 3160
BbsI	GAAGAC	4 cut(s)	440, 555, 1869, 2975
Bbv12I	GWGCWC	5 cut(s)	1362, 1636, 2119, 3015, 3679
BceAI	ACGGC	5 cut(s)	1475, 2793, 3236, 3479, 4031
BcgI	CGANNNNNNTGC	2 cut(s)	3758, 3792
BciT130I	CCWGG	8 cut(s)	204, 841, 2264, 3153, 3195, 3366, 3479, 3969
BciVI	GTATCC	1 cut(s)	3169
BclI	TGATCA	4 cut(s)	1188, 2061, 2389, 3736
BcoDI	GTCTC	5 cut(s)	681, 943, 1945, 3710, 3872
BfmI	CTRYAG	5 cut(s)	282, 2049, 2493, 3783, 4080
BfrI	CTTAAG	1 cut(s)	1799
BfuAI	ACCTGC	1 cut(s)	3553
BfuI	GTATCC	1 cut(s)	3169
BglI	GCCNNNNNGGC	1 cut(s)	4052
Bme1390I	CCNGG	8 cut(s)	204, 841, 2264, 3153, 3195, 3366, 3479, 3969
Bme18I	GGWCC	3 cut(s)	843, 3301, 3419
BmeRI	GACNNNNNGTC	2 cut(s)	1204, 3723
BmeT110I	CYCGRG	2 cut(s)	2016, 2166
BmgT120I	GGNCC	4 cut(s)	418, 843, 3301, 3419
BmrFI	CCNGG	8 cut(s)	204, 841, 2264, 3153, 3195, 3366, 3479, 3969
BmrI	ACTGGG	1 cut(s)	2578
BmsI	GCATC	8 cut(s)	206, 289, 709, 808, 926, 1375, 3102, 3481
BmuI	ACTGGG	1 cut(s)	2578
BpiI	GAAGAC	4 cut(s)	440, 555, 1869, 2975
BplI	GAGNNNNNCTC	4 cut(s)	181, 213, 923, 955
BpmI	CTGGAG	3 cut(s)	232, 807, 1325
Bpu10I	CCTNAGC	1 cut(s)	2029
BpuEI	CTTGAG	2 cut(s)	3896, 4011
Bsa29I	ATCGAT	2 cut(s)	1969, 3808
BsaAI	YACGTR	1 cut(s)	1550
BsaBI	GATNNNNATC	5 cut(s)	1437, 2082, 2388, 2676, 3804
BsaI	GGTCTC	1 cut(s)	681
BsaWI	WCCGGW	2 cut(s)	51, 2762
BsaXI	ACNNNNNCTCC	4 cut(s)	1318, 1348, 3456, 3486
Bsc4I	CCNNNNNNNGG	6 cut(s)	203, 647, 3068, 3365, 4127, 4128
Bse118I	RCCGGY	1 cut(s)	1328
Bse1I	ACTGG	5 cut(s)	215, 2385, 2573, 2945, 3486
Bse21I	CCTNAGG	1 cut(s)	3305
Bse3DI	GCAATG	2 cut(s)	373, 3239
Bse8I	GATNNNNATC	5 cut(s)	1437, 2082, 2388, 2676, 3804
BseAI	TCCGGA	2 cut(s)	51, 2762
BseBI	CCWGG	8 cut(s)	204, 841, 2264, 3153, 3195, 3366, 3479, 3969
BseCI	ATCGAT	2 cut(s)	1969, 3808
BseJI	GATNNNNATC	5 cut(s)	1437, 2082, 2388, 2676, 3804
BseLI	CCNNNNNNNGG	6 cut(s)	203, 647, 3068, 3365, 4127, 4128
BseMI	GCAATG	2 cut(s)	373, 3239
BseMII	CTCAG	8 cut(s)	474, 1938, 3088, 3296, 3521, 3660, 3978, 4053
BseNI	ACTGG	5 cut(s)	215, 2385, 2573, 2945, 3486
BseRI	GAGGAG	3 cut(s)	2516, 2818, 4014
BseSI	GKGCMC	4 cut(s)	1009, 1636, 3679, 3933
BseYI	CCCAGC	4 cut(s)	310, 1388, 2021, 2396
Bsh1236I	CGCG	2 cut(s)	85, 1051
BshFI	GGCC	2 cut(s)	419, 2450
BshNI	GGYRCC	1 cut(s)	1006
BshVI	ATCGAT	2 cut(s)	1969, 3808
BsiHKAI	GWGCWC	5 cut(s)	1362, 1636, 2119, 3015, 3679
BsiHKCI	CYCGRG	2 cut(s)	2016, 2166
BsiSI	CCGG	4 cut(s)	52, 641, 1329, 2763
BslFI	GGGAC	4 cut(s)	57, 372, 756, 894
BslI	CCNNNNNNNGG	6 cut(s)	203, 647, 3068, 3365, 4127, 4128
BsmAI	GTCTC	5 cut(s)	681, 943, 1945, 3710, 3872
BsmFI	GGGAC	4 cut(s)	57, 372, 756, 894
BsmI	GAATGC	1 cut(s)	3688
BsnI	GGCC	2 cut(s)	419, 2450
Bso31I	GGTCTC	1 cut(s)	681
BsoBI	CYCGRG	2 cut(s)	2016, 2166
Bsp13I	TCCGGA	2 cut(s)	51, 2762
Bsp1407I	TGTACA	1 cut(s)	2902
Bsp19I	CCATGG	2 cut(s)	723, 2523
BspACI	CCGC	5 cut(s)	85, 117, 3450, 3996, 3999
BspANI	GGCC	2 cut(s)	419, 2450
BspCNI	CTCAG	8 cut(s)	475, 1939, 3087, 3297, 3520, 3661, 3979, 4054
BspDI	ATCGAT	2 cut(s)	1969, 3808
BspEI	TCCGGA	2 cut(s)	51, 2762
BspFNI	CGCG	2 cut(s)	85, 1051
BspMAI	CTGCAG	3 cut(s)	2053, 3787, 4084
BspMI	ACCTGC	1 cut(s)	3553
BspPI	GGATC	6 cut(s)	1160, 1718, 2302, 2391, 2850, 3794
BspQI	GCTCTTC	2 cut(s)	698, 1045
BspT107I	GGYRCC	1 cut(s)	1006
BspTI	CTTAAG	1 cut(s)	1799
BspTNI	GGTCTC	1 cut(s)	681
BsrDI	GCAATG	2 cut(s)	373, 3239
BsrFI	RCCGGY	1 cut(s)	1328
BsrGI	TGTACA	1 cut(s)	2902
BsrI	ACTGG	5 cut(s)	215, 2385, 2573, 2945, 3486
BssAI	RCCGGY	1 cut(s)	1328
BssT1I	CCWWGG	2 cut(s)	723, 2523
Bst2UI	CCWGG	8 cut(s)	204, 841, 2264, 3153, 3195, 3366, 3479, 3969
BstAFI	CTTAAG	1 cut(s)	1799
BstAPI	GCANNNNNTGC	1 cut(s)	3683
BstAUI	TGTACA	1 cut(s)	2902
BstBAI	YACGTR	1 cut(s)	1550
BstC8I	GCNNGC	9 cut(s)	541, 1053, 1142, 1673, 2545, 2959, 3249, 3282, 4044
BstDSI	CCRYGG	4 cut(s)	723, 2523, 3711, 4122
BstEII	GGTNACC	1 cut(s)	2518
BstFNI	CGCG	2 cut(s)	85, 1051
BstHHI	GCGC	3 cut(s)	276, 1051, 1671
BstMAI	GTCTC	5 cut(s)	681, 943, 1945, 3710, 3872
BstMWI	GCNNNNNNNGC	9 cut(s)	416, 914, 1004, 1366, 1855, 3559, 3683, 3785, 4052
BstNI	CCWGG	8 cut(s)	204, 841, 2264, 3153, 3195, 3366, 3479, 3969
BstNSI	RCATGY	3 cut(s)	150, 1849, 4046
BstPI	GGTNACC	1 cut(s)	2518
BstSCI	CCNGG	8 cut(s)	202, 839, 2262, 3151, 3193, 3364, 3477, 3967
BstSFI	CTRYAG	5 cut(s)	282, 2049, 2493, 3783, 4080
BstSLI	GKGCMC	4 cut(s)	1009, 1636, 3679, 3933
BstUI	CGCG	2 cut(s)	85, 1051
BstV2I	GAAGAC	4 cut(s)	440, 555, 1869, 2975
BstX2I	RGATCY	2 cut(s)	1723, 2855
BstXI	CCANNNNNNTGG	1 cut(s)	1224
BstYI	RGATCY	2 cut(s)	1723, 2855
Bsu15I	ATCGAT	2 cut(s)	1969, 3808
Bsu36I	CCTNAGG	1 cut(s)	3305
BsuI	GTATCC	1 cut(s)	3169
BsuRI	GGCC	2 cut(s)	419, 2450
BsuTUI	ATCGAT	2 cut(s)	1969, 3808
BtgI	CCRYGG	4 cut(s)	723, 2523, 3711, 4122
BtsIMutI	CAGTG	1 cut(s)	3493
BveI	ACCTGC	1 cut(s)	3553
Cac8I	GCNNGC	9 cut(s)	541, 1053, 1142, 1673, 2545, 2959, 3249, 3282, 4044
CaiI	CAGNNNCTG	3 cut(s)	2492, 4061, 4088
CfoI	GCGC	3 cut(s)	276, 1051, 1671
Cfr10I	RCCGGY	1 cut(s)	1328
Cfr13I	GGNCC	4 cut(s)	418, 843, 3301, 3419
ClaI	ATCGAT	2 cut(s)	1969, 3808
CseI	GACGC	2 cut(s)	91, 2369
Csp6I	GTAC	5 cut(s)	1872, 1915, 2903, 3059, 3089
CviQI	GTAC	5 cut(s)	1872, 1915, 2903, 3059, 3089
DraI	TTTAAA	2 cut(s)	91, 2445
DriI	GACNNNNNGTC	2 cut(s)	1204, 3723
Eam1105I	GACNNNNNGTC	2 cut(s)	1204, 3723
EciI	GGCGGA	1 cut(s)	132
Ecl136II	GAGCTC	2 cut(s)	1360, 2117
Eco130I	CCWWGG	2 cut(s)	723, 2523
Eco147I	AGGCCT	1 cut(s)	2450
Eco24I	GRGCYC	6 cut(s)	317, 1362, 2119, 2829, 3075, 3160
Eco31I	GGTCTC	1 cut(s)	681
Eco32I	GATATC	2 cut(s)	1641, 2257
Eco47I	GGWCC	3 cut(s)	843, 3301, 3419
Eco53kI	GAGCTC	2 cut(s)	1360, 2117
Eco57I	CTGAAG	2 cut(s)	2754, 3042
Eco81I	CCTNAGG	1 cut(s)	3305
Eco88I	CYCGRG	2 cut(s)	2016, 2166
Eco91I	GGTNACC	1 cut(s)	2518
EcoICRI	GAGCTC	2 cut(s)	1360, 2117
EcoO109I	RGGNCCY	2 cut(s)	3301, 3419
EcoO65I	GGTNACC	1 cut(s)	2518
EcoRI	GAATTC	3 cut(s)	97, 531, 663
EcoRII	CCWGG	8 cut(s)	202, 839, 2262, 3151, 3193, 3364, 3477, 3967
EcoRV	GATATC	2 cut(s)	1641, 2257
EcoT14I	CCWWGG	2 cut(s)	723, 2523
EcoT22I	ATGCAT	1 cut(s)	1377
EcoT38I	GRGCYC	6 cut(s)	317, 1362, 2119, 2829, 3075, 3160
ErhI	CCWWGG	2 cut(s)	723, 2523
FaqI	GGGAC	4 cut(s)	57, 372, 756, 894
FbaI	TGATCA	4 cut(s)	1188, 2061, 2389, 3736
FblI	GTMKAC	2 cut(s)	2533, 3756
FriOI	GRGCYC	6 cut(s)	317, 1362, 2119, 2829, 3075, 3160
FspI	TGCGCA	1 cut(s)	1670
GlaI	GCGC	3 cut(s)	275, 1050, 1670
GsaI	CCCAGC	4 cut(s)	314, 1392, 2025, 2400
GsuI	CTGGAG	3 cut(s)	232, 807, 1325
HaeIII	GGCC	2 cut(s)	419, 2450
HapII	CCGG	4 cut(s)	52, 641, 1329, 2763
HgaI	GACGC	2 cut(s)	91, 2369
HhaI	GCGC	3 cut(s)	276, 1051, 1671
Hin6I	GCGC	3 cut(s)	274, 1049, 1669
HinP1I	GCGC	3 cut(s)	274, 1049, 1669
HincII	GTYRAC	2 cut(s)	2314, 2534
HindII	GTYRAC	2 cut(s)	2314, 2534
HindIII	AAGCTT	3 cut(s)	408, 1673, 1734
HpaII	CCGG	4 cut(s)	52, 641, 1329, 2763
HphI	GGTGA	9 cut(s)	302, 1804, 2071, 2723, 2759, 3130, 3407, 3650, 4043
Hpy99I	CGWCG	4 cut(s)	1626, 1896, 3851, 4052
HpyCH4IV	ACGT	7 cut(s)	39, 427, 832, 1205, 1549, 3609, 3846
HpyF10VI	GCNNNNNNNGC	9 cut(s)	416, 914, 1004, 1366, 1855, 3559, 3683, 3785, 4052
HpySE526I	ACGT	7 cut(s)	39, 427, 832, 1205, 1549, 3609, 3846
HspAI	GCGC	3 cut(s)	274, 1049, 1669
Kpn2I	TCCGGA	2 cut(s)	51, 2762
Ksp22I	TGATCA	4 cut(s)	1188, 2061, 2389, 3736
LguI	GCTCTTC	2 cut(s)	698, 1045
LweI	GCATC	8 cut(s)	206, 289, 709, 808, 926, 1375, 3102, 3481
MaeII	ACGT	7 cut(s)	39, 427, 832, 1205, 1549, 3609, 3846
MaeIII	GTNAC	9 cut(s)	79, 1206, 1601, 2161, 2518, 3455, 3725, 3791, 4031
MfeI	CAATTG	1 cut(s)	1772
MflI	RGATCY	2 cut(s)	1723, 2855
MlyI	GAGTC	7 cut(s)	13, 722, 1193, 3029, 3910, 3998, 4052
MmeI	TCCRAC	4 cut(s)	2215, 2397, 3065, 3634
Mph1103I	ATGCAT	1 cut(s)	1377
MroI	TCCGGA	2 cut(s)	51, 2762
MroXI	GAANNNNTTC	2 cut(s)	1413, 2770
MslI	CAYNNNNRTG	3 cut(s)	2355, 3267, 3435
MspCI	CTTAAG	1 cut(s)	1799
MspI	CCGG	4 cut(s)	52, 641, 1329, 2763
MspR9I	CCNGG	8 cut(s)	204, 841, 2264, 3153, 3195, 3366, 3479, 3969
MunI	CAATTG	1 cut(s)	1772
Mva1269I	GAATGC	1 cut(s)	3688
MvaI	CCWGG	8 cut(s)	204, 841, 2264, 3153, 3195, 3366, 3479, 3969
MvnI	CGCG	2 cut(s)	85, 1051
MwoI	GCNNNNNNNGC	9 cut(s)	416, 914, 1004, 1366, 1855, 3559, 3683, 3785, 4052
NcoI	CCATGG	2 cut(s)	723, 2523
NmuCI	GTSAC	6 cut(s)	79, 1206, 3455, 3725, 3791, 4031
NsbI	TGCGCA	1 cut(s)	1670
NsiI	ATGCAT	1 cut(s)	1377
NspI	RCATGY	3 cut(s)	150, 1849, 4046
PaeI	GCATGC	1 cut(s)	4046
PaeR7I	CTCGAG	1 cut(s)	2016
PasI	CCCWGGG	1 cut(s)	3478
PceI	AGGCCT	1 cut(s)	2450
PciI	ACATGT	1 cut(s)	146
PciSI	GCTCTTC	2 cut(s)	698, 1045
PctI	GAATGC	1 cut(s)	3688
PdmI	GAANNNNTTC	2 cut(s)	1413, 2770
PleI	GAGTC	7 cut(s)	12, 722, 1193, 3029, 3910, 3997, 4052
PpsI	GAGTC	7 cut(s)	12, 722, 1193, 3029, 3910, 3997, 4052
Ppu21I	YACGTR	1 cut(s)	1550
PpuMI	RGGWCCY	2 cut(s)	3301, 3419
PscI	ACATGT	1 cut(s)	146
PshBI	ATTAAT	1 cut(s)	1886
PsiI	TTATAA	2 cut(s)	3350, 3831
Psp124BI	GAGCTC	2 cut(s)	1362, 2119
Psp1406I	AACGTT	1 cut(s)	3609
Psp5II	RGGWCCY	2 cut(s)	3301, 3419
Psp6I	CCWGG	8 cut(s)	202, 839, 2262, 3151, 3193, 3364, 3477, 3967
PspEI	GGTNACC	1 cut(s)	2518
PspFI	CCCAGC	4 cut(s)	310, 1388, 2021, 2396
PspGI	CCWGG	8 cut(s)	202, 839, 2262, 3151, 3193, 3364, 3477, 3967
PspPI	GGNCC	4 cut(s)	418, 843, 3301, 3419
PspPPI	RGGWCCY	2 cut(s)	3301, 3419
PspXI	VCTCGAGB	1 cut(s)	2016
PsrI	GAACNNNNNNTAC	2 cut(s)	1530, 1562
PstI	CTGCAG	3 cut(s)	2053, 3787, 4084
PstNI	CAGNNNCTG	3 cut(s)	2492, 4061, 4088
PsuI	RGATCY	2 cut(s)	1723, 2855
RsaI	GTAC	5 cut(s)	1873, 1916, 2904, 3060, 3090
RsaNI	GTAC	5 cut(s)	1872, 1915, 2903, 3059, 3089
RseI	CAYNNNNRTG	3 cut(s)	2355, 3267, 3435
SacI	GAGCTC	2 cut(s)	1362, 2119
SalI	GTCGAC	1 cut(s)	2532
SapI	GCTCTTC	2 cut(s)	698, 1045
Sau96I	GGNCC	4 cut(s)	418, 843, 3301, 3419
SchI	GAGTC	7 cut(s)	13, 722, 1193, 3029, 3910, 3998, 4052
ScrFI	CCNGG	8 cut(s)	204, 841, 2264, 3153, 3195, 3366, 3479, 3969
SfaNI	GCATC	8 cut(s)	206, 289, 709, 808, 926, 1375, 3102, 3481
SfcI	CTRYAG	5 cut(s)	282, 2049, 2493, 3783, 4080
Sfr274I	CTCGAG	1 cut(s)	2016
SinI	GGWCC	3 cut(s)	843, 3301, 3419
SlaI	CTCGAG	1 cut(s)	2016
SmiMI	CAYNNNNRTG	3 cut(s)	2355, 3267, 3435
SmlI	CTYRAG	4 cut(s)	1799, 2016, 3875, 4026
SmoI	CTYRAG	4 cut(s)	1799, 2016, 3875, 4026
SphI	GCATGC	1 cut(s)	4046
SseBI	AGGCCT	1 cut(s)	2450
SsiI	CCGC	5 cut(s)	85, 117, 3450, 3996, 3999
SspI	AATATT	2 cut(s)	566, 3693
SstI	GAGCTC	2 cut(s)	1362, 2119
StuI	AGGCCT	1 cut(s)	2450
StyD4I	CCNGG	8 cut(s)	202, 839, 2262, 3151, 3193, 3364, 3477, 3967
StyI	CCWWGG	2 cut(s)	723, 2523
TaiI	ACGT	7 cut(s)	42, 430, 835, 1208, 1552, 3612, 3849
TatI	WGTACW	3 cut(s)	1871, 2902, 3088
TauI	GCSGC	1 cut(s)	3999
TscAI	CASTG	1 cut(s)	3493
TseFI	GTSAC	6 cut(s)	79, 1206, 3455, 3725, 3791, 4031
Tsp45I	GTSAC	6 cut(s)	79, 1206, 3455, 3725, 3791, 4031
TspGWI	ACGGA	6 cut(s)	792, 1677, 1936, 2200, 3700, 4050
TspRI	CASTG	1 cut(s)	3493
Vha464I	CTTAAG	1 cut(s)	1799
VneI	GTGCAC	2 cut(s)	1632, 3675
VpaK11BI	GGWCC	3 cut(s)	843, 3301, 3419
VspI	ATTAAT	1 cut(s)	1886
XbaI	TCTAGA	1 cut(s)	1991
XceI	RCATGY	3 cut(s)	150, 1849, 4046
XhoI	CTCGAG	1 cut(s)	2016
XmiI	GTMKAC	2 cut(s)	2533, 3756
XmnI	GAANNNNTTC	2 cut(s)	1413, 2770
Zsp2I	ATGCAT	1 cut(s)	1377

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.