AT2G27315

Prolamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002420
Physical Location & Seq
Reverse (-)
11689467 .. 11690004
538 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G27315.1

Sequence Viewer

Length: 363 bp
ATGGGAAGCAAAGCTATTGTATCAGTCTTGTTAATGGCATCCCTTTGTGGTACCATTTTTGTTACACAAGGAGTAGCTGAAATGCAAAAGCCACCGATGTTTCCAGGACTTTTCCAACCAGGCTCACCCGTTGATCTTGTAAAATGTATGTCGTCTCTCTCCAACGTTGAAGGATGTGTGCTCGAAATCTCTAAATCAATTTTCTCCGGCAAATTTGAAAATATTGAAGGCGCATGTTGCAAGGCGTTTTCGGCCTTAGATGCCAACTGTTGGCCCGAAATGTTTCCGTTGAATCCGTTTTTCCCTCCTCTCCTCAAGGATATTTGTGCACGTATTGTCCCAAAATCTCCTACACATAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

120

Amino Acids

12.9

Weight (kDa)

6.53

Isoelectric Point (pI)

48.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 48 - 109 1.7e-14 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 50
AccB1I GGYRCC 1 cut(s) 50
AccB7I CCANNNNNTGG 1 cut(s) 270
AclI AACGTT 1 cut(s) 165
AcsI RAATTY 1 cut(s) 212
AfaI GTAC 1 cut(s) 52
AfiI CCNNNNNNNGG 1 cut(s) 270
AgsI TTSAA 4 cut(s) 170, 218, 227, 292
AjnI CCWGG 2 cut(s) 103, 118
AluBI AGCT 2 cut(s) 14, 77
AluI AGCT 2 cut(s) 14, 77
Alw21I GWGCWC 2 cut(s) 183, 331
Alw26I GTCTC 1 cut(s) 159
Alw44I GTGCAC 1 cut(s) 327
AoxI GGCC 2 cut(s) 252, 272
ApaLI GTGCAC 1 cut(s) 327
ApoI RAATTY 1 cut(s) 212
Asp700I GAANNNNTTC 1 cut(s) 282
Asp718I GGTACC 1 cut(s) 50
AspLEI GCGC 1 cut(s) 233
AspS9I GGNCC 1 cut(s) 273
AsuHPI GGTGA 1 cut(s) 117
BaeGI GKGCMC 1 cut(s) 331
BanI GGYRCC 1 cut(s) 50
Bbv12I GWGCWC 2 cut(s) 183, 331
BciT130I CCWGG 2 cut(s) 105, 120
BcoDI GTCTC 1 cut(s) 159
Bme1390I CCNGG 2 cut(s) 105, 120
BmgT120I GGNCC 1 cut(s) 273
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 2 cut(s) 105, 120
BmsI GCATC 2 cut(s) 47, 250
BpuEI CTTGAG 1 cut(s) 299
BsaAI YACGTR 1 cut(s) 332
Bsc4I CCNNNNNNNGG 1 cut(s) 270
BseBI CCWGG 2 cut(s) 105, 120
BseGI GGATG 2 cut(s) 38, 179
BseLI CCNNNNNNNGG 1 cut(s) 270
BseRI GAGGAG 2 cut(s) 297, 302
BseSI GKGCMC 1 cut(s) 331
BshFI GGCC 2 cut(s) 254, 274
BshNI GGYRCC 1 cut(s) 50
BsiHKAI GWGCWC 2 cut(s) 183, 331
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 1 cut(s) 323
BslI CCNNNNNNNGG 1 cut(s) 270
BsmAI GTCTC 1 cut(s) 159
BsmBI CGTCTC 1 cut(s) 159
BsmFI GGGAC 1 cut(s) 323
BsnI GGCC 2 cut(s) 254, 274
Bsp1286I GDGCHC 2 cut(s) 183, 331
Bsp143I GATC 1 cut(s) 133
BspANI GGCC 2 cut(s) 254, 274
BspLI GGNNCC 1 cut(s) 52
BspT107I GGYRCC 1 cut(s) 50
BssMI GATC 1 cut(s) 133
Bst2UI CCWGG 2 cut(s) 105, 120
Bst4CI ACNGT 1 cut(s) 269
BstBAI YACGTR 1 cut(s) 332
BstDEI CTNAG 1 cut(s) 256
BstF5I GGATG 2 cut(s) 38, 179
BstHHI GCGC 1 cut(s) 233
BstKTI GATC 1 cut(s) 136
BstMAI GTCTC 1 cut(s) 159
BstMBI GATC 1 cut(s) 133
BstMWI GCNNNNNNNGC 3 cut(s) 237, 251, 260
BstNI CCWGG 2 cut(s) 105, 120
BstNSI RCATGY 1 cut(s) 237
BstSCI CCNGG 2 cut(s) 103, 118
BstSLI GKGCMC 1 cut(s) 331
BsuRI GGCC 2 cut(s) 254, 274
BtsCI GGATG 2 cut(s) 38, 179
CfoI GCGC 1 cut(s) 233
Cfr13I GGNCC 1 cut(s) 273
Csp6I GTAC 1 cut(s) 51
CviAII CATG 1 cut(s) 234
CviJI RGCY 6 cut(s) 14, 77, 91, 123, 254, 274
CviKI_1 RGCY 6 cut(s) 14, 77, 91, 123, 254, 274
CviQI GTAC 1 cut(s) 51
DdeI CTNAG 1 cut(s) 256
DpnI GATC 1 cut(s) 135
DpnII GATC 1 cut(s) 133
EcoRII CCWGG 2 cut(s) 103, 118
Esp3I CGTCTC 1 cut(s) 159
FaeI CATG 1 cut(s) 237
FaiI YATR 3 cut(s) 149, 235, 357
FaqI GGGAC 1 cut(s) 323
FatI CATG 1 cut(s) 233
FokI GGATG 2 cut(s) 25, 186
GlaI GCGC 1 cut(s) 232
HaeIII GGCC 2 cut(s) 254, 274
HapII CCGG 1 cut(s) 207
HhaI GCGC 1 cut(s) 233
Hin1II CATG 1 cut(s) 237
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HinfI GANTC 1 cut(s) 292
HpaII CCGG 1 cut(s) 207
HphI GGTGA 1 cut(s) 117
Hpy166II GTNNAC 1 cut(s) 329
Hpy8I GTNNAC 1 cut(s) 329
HpyAV CCTTC 2 cut(s) 164, 221
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4IV ACGT 2 cut(s) 165, 331
HpyCH4V TGCA 3 cut(s) 85, 240, 329
HpyF10VI GCNNNNNNNGC 3 cut(s) 237, 251, 260
HpyF3I CTNAG 1 cut(s) 256
HpySE526I ACGT 2 cut(s) 165, 331
Hsp92II CATG 1 cut(s) 237
HspAI GCGC 1 cut(s) 231
KpnI GGTACC 1 cut(s) 54
Kzo9I GATC 1 cut(s) 133
LpnPI CCDG 5 cut(s) 90, 105, 117, 132, 220
LweI GCATC 2 cut(s) 47, 250
MaeII ACGT 2 cut(s) 165, 331
MaeIII GTNAC 1 cut(s) 61
MalI GATC 1 cut(s) 135
MboI GATC 1 cut(s) 133
MhlI GDGCHC 2 cut(s) 183, 331
MluCI AATT 2 cut(s) 198, 212
MmeI TCCRAC 2 cut(s) 139, 186
MnlI CCTC 3 cut(s) 315, 318, 323
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 1 cut(s) 32
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 2 cut(s) 105, 120
MvaI CCWGG 2 cut(s) 105, 120
MwoI GCNNNNNNNGC 3 cut(s) 237, 251, 260
NdeII GATC 1 cut(s) 133
NlaIII CATG 1 cut(s) 237
NlaIV GGNNCC 1 cut(s) 52
NspI RCATGY 1 cut(s) 237
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 1 cut(s) 292
PflMI CCANNNNNTGG 1 cut(s) 270
PfoI TCCNGGA 1 cut(s) 103
Ppu21I YACGTR 1 cut(s) 332
Psp1406I AACGTT 1 cut(s) 165
Psp6I CCWGG 2 cut(s) 103, 118
PspGI CCWGG 2 cut(s) 103, 118
PspN4I GGNNCC 1 cut(s) 52
PspPI GGNCC 1 cut(s) 273
RsaI GTAC 1 cut(s) 52
RsaNI GTAC 1 cut(s) 51
SaqAI TTAA 1 cut(s) 32
Sau3AI GATC 1 cut(s) 133
Sau96I GGNCC 1 cut(s) 273
ScrFI CCNGG 2 cut(s) 105, 120
SduI GDGCHC 2 cut(s) 183, 331
SetI ASST 4 cut(s) 16, 79, 168, 334
SfaNI GCATC 2 cut(s) 47, 250
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse9I AATT 2 cut(s) 198, 212
SspI AATATT 1 cut(s) 223
StyD4I CCNGG 2 cut(s) 103, 118
TaaI ACNGT 1 cut(s) 269
TaiI ACGT 2 cut(s) 168, 334
TaqI TCGA 1 cut(s) 183
TasI AATT 2 cut(s) 198, 212
TfiI GAWTC 1 cut(s) 292
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TspGWI ACGGA 2 cut(s) 276, 285
Van91I CCANNNNNTGG 1 cut(s) 270
VneI GTGCAC 1 cut(s) 327
XapI RAATTY 1 cut(s) 212
XceI RCATGY 1 cut(s) 237
XmnI GAANNNNTTC 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.