AT5G54062

Prolamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002420
Physical Location & Seq
Forward (+)
21938793 .. 21939637
845 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G54062.1

Sequence Viewer

Length: 624 bp
ATGGAAGGTAAGATCCAAGCTCTTCTCTCGACGATTCTAGTTGTGACTTTGTGTGCCACTACTTTAGTGAAGCCCGGGCTAGCTCAACTCCCTACCAGTGCTGGGTTTTCTCTACCCGGCTCCCAAGTTGATCTCGCAAAATGTTGGTCATCGCTCCTCAACATTCACGGTTGCAATATCGAAATCTTTAAATCTGTTTTAACCGGTAAGTTTGAAAACGTTGGATCCACGTGCTGCAAGGCTTTTACAGAAGTGGATGCAAAGTGTTGGCCAAAAATGTTTCCGTTGAATCCATTATTCCCTCCTCTTCTCAAGGATGGTTGCTCTCGCATCATCTCAGGTGCACCAGCACACAAGACACCGCAATTCCCTGTCATCCCTGGTTCTCCGATCGATCTCACAAAATGTTTGTCATCACTTGTCAACGTTCAAGGTTGTGTAACTGAAATCTACAAATCAGTTTTCACAGGAAAGTGTGATAACGTAGGATTTATGTGCTGCAAGGCGTTTATGGCTGTGGATGCAAAATGTTGGCCAAAAATGTTTCCACTAAATCCGTTCTTCCCTCCTCTCCTCAAAAATGTATGTTCTCGCATCAACACATCTGCTCCTACACACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

207

Amino Acids

22.33

Weight (kDa)

8.94

Isoelectric Point (pI)

42.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 47 - 109 2.3e-15 Prolamin-like
Prolamin_like PF05617 135 - 197 9.3e-14 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 362
AclI AACGTT 2 cut(s) 219, 426
AclWI GGATC 3 cut(s) 7, 219, 232
AcoI YGGCCR 2 cut(s) 269, 533
AcvI CACGTG 1 cut(s) 231
AfiI CCNNNNNNNGG 1 cut(s) 102
AgeI ACCGGT 1 cut(s) 203
AgsI TTSAA 3 cut(s) 215, 289, 431
AjnI CCWGG 1 cut(s) 379
AleI CACNNNNGTG 1 cut(s) 619
AluBI AGCT 2 cut(s) 20, 83
AluI AGCT 2 cut(s) 20, 83
Alw21I GWGCWC 1 cut(s) 346
Alw44I GTGCAC 1 cut(s) 342
AlwI GGATC 3 cut(s) 7, 219, 232
Ama87I CYCGRG 1 cut(s) 74
AoxI GGCC 2 cut(s) 269, 533
ApaLI GTGCAC 1 cut(s) 342
ApeKI GCWGC 2 cut(s) 234, 498
ArsI GACNNNNNNTTYG 4 cut(s) 131, 163, 395, 427
AsiGI ACCGGT 1 cut(s) 203
AsuC2I CCSGG 3 cut(s) 75, 76, 117
AsuNHI GCTAGC 1 cut(s) 79
AvaI CYCGRG 1 cut(s) 74
BaeGI GKGCMC 1 cut(s) 346
BalI TGGCCA 2 cut(s) 271, 535
BamHI GGATCC 1 cut(s) 224
BbrPI CACGTG 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 346
BbvI GCAGC 2 cut(s) 221, 485
BccI CCATC 1 cut(s) 311
BciT130I CCWGG 1 cut(s) 381
BcnI CCSGG 3 cut(s) 75, 76, 117
BfaI CTAG 2 cut(s) 38, 80
BisI GCNGC 2 cut(s) 235, 499
BlsI GCNGC 2 cut(s) 236, 500
Bme1390I CCNGG 4 cut(s) 75, 76, 117, 381
BmeT110I CYCGRG 1 cut(s) 74
BmiI GGNNCC 2 cut(s) 121, 226
BmrFI CCNGG 4 cut(s) 75, 76, 117, 381
BmsI GCATC 4 cut(s) 247, 339, 511, 603
BmtI GCTAGC 1 cut(s) 83
BpuEI CTTGAG 1 cut(s) 296
BpuMI CCSGG 3 cut(s) 75, 76, 117
Bsa29I ATCGAT 1 cut(s) 393
BsaAI YACGTR 1 cut(s) 231
BsaJI CCNNGG 2 cut(s) 74, 379
BsaWI WCCGGW 1 cut(s) 203
BsaXI ACNNNNNCTCC 2 cut(s) 592, 622
Bsc4I CCNNNNNNNGG 1 cut(s) 102
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 96
BseBI CCWGG 1 cut(s) 381
BseCI ATCGAT 1 cut(s) 393
BseDI CCNNGG 2 cut(s) 74, 379
BseGI GGATG 4 cut(s) 262, 322, 375, 526
BseLI CCNNNNNNNGG 1 cut(s) 102
BseMII CTCAG 1 cut(s) 351
BseNI ACTGG 1 cut(s) 96
BseRI GAGGAG 4 cut(s) 146, 294, 558, 563
BseSI GKGCMC 1 cut(s) 346
BseXI GCAGC 2 cut(s) 221, 485
BseYI CCCAGC 1 cut(s) 101
Bsh1285I CGRYCG 1 cut(s) 393
BshFI GGCC 2 cut(s) 271, 535
BshTI ACCGGT 1 cut(s) 203
BshVI ATCGAT 1 cut(s) 393
BsiEI CGRYCG 1 cut(s) 393
BsiHKAI GWGCWC 1 cut(s) 346
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 3 cut(s) 75, 117, 204
BslI CCNNNNNNNGG 1 cut(s) 102
BsnI GGCC 2 cut(s) 271, 535
BsoBI CYCGRG 1 cut(s) 74
Bsp1286I GDGCHC 1 cut(s) 346
Bsp143I GATC 5 cut(s) 12, 130, 224, 390, 394
BspACI CCGC 1 cut(s) 362
BspANI GGCC 2 cut(s) 271, 535
BspCNI CTCAG 1 cut(s) 350
BspDI ATCGAT 1 cut(s) 393
BspLI GGNNCC 2 cut(s) 121, 226
BspOI GCTAGC 1 cut(s) 83
BspPI GGATC 3 cut(s) 7, 219, 232
BspQI GCTCTTC 1 cut(s) 27
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 1 cut(s) 96
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 2 cut(s) 74, 379
BssMI GATC 5 cut(s) 12, 130, 224, 390, 394
Bst2UI CCWGG 1 cut(s) 381
Bst4CI ACNGT 1 cut(s) 170
Bst6I CTCTTC 2 cut(s) 27, 312
BstBAI YACGTR 1 cut(s) 231
BstC8I GCNNGC 1 cut(s) 81
BstDEI CTNAG 1 cut(s) 337
BstF5I GGATG 4 cut(s) 262, 322, 375, 526
BstKTI GATC 5 cut(s) 15, 133, 227, 393, 397
BstMBI GATC 5 cut(s) 12, 130, 224, 390, 394
BstMCI CGRYCG 1 cut(s) 393
BstMWI GCNNNNNNNGC 2 cut(s) 512, 521
BstNI CCWGG 1 cut(s) 381
BstSCI CCNGG 4 cut(s) 73, 74, 115, 379
BstSLI GKGCMC 1 cut(s) 346
BstV1I GCAGC 2 cut(s) 221, 485
BstX2I RGATCY 2 cut(s) 12, 224
BstYI RGATCY 2 cut(s) 12, 224
Bsu15I ATCGAT 1 cut(s) 393
BsuRI GGCC 2 cut(s) 271, 535
BsuTUI ATCGAT 1 cut(s) 393
BtgZI GCGATG 1 cut(s) 135
BtsCI GGATG 4 cut(s) 262, 322, 375, 526
BtsIMutI CAGTG 1 cut(s) 103
Cac8I GCNNGC 1 cut(s) 81
Cfr10I RCCGGY 1 cut(s) 203
Cfr9I CCCGGG 1 cut(s) 74
ClaI ATCGAT 1 cut(s) 393
CspAI ACCGGT 1 cut(s) 203
CviJI RGCY 9 cut(s) 20, 73, 79, 83, 120, 242, 271, 515, 535
CviKI_1 RGCY 9 cut(s) 20, 73, 79, 83, 120, 242, 271, 515, 535
DdeI CTNAG 1 cut(s) 337
DpnI GATC 5 cut(s) 14, 132, 226, 392, 396
DpnII GATC 5 cut(s) 12, 130, 224, 390, 394
DraI TTTAAA 1 cut(s) 190
EaeI YGGCCR 2 cut(s) 269, 533
Eam1104I CTCTTC 2 cut(s) 27, 312
EarI CTCTTC 2 cut(s) 27, 312
Eco72I CACGTG 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 379
FaiI YATR 3 cut(s) 494, 512, 586
Fnu4HI GCNGC 2 cut(s) 235, 499
FokI GGATG 4 cut(s) 269, 329, 362, 533
Fsp4HI GCNGC 2 cut(s) 235, 499
FspBI CTAG 2 cut(s) 38, 80
GluI GCNGC 2 cut(s) 235, 499
GsaI CCCAGC 1 cut(s) 105
HaeIII GGCC 2 cut(s) 271, 535
HapII CCGG 3 cut(s) 75, 117, 204
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HinfI GANTC 2 cut(s) 34, 289
HpaII CCGG 3 cut(s) 75, 117, 204
Hpy166II GTNNAC 2 cut(s) 344, 424
Hpy188I TCNGA 1 cut(s) 390
Hpy188III TCNNGA 1 cut(s) 28
Hpy8I GTNNAC 2 cut(s) 344, 424
Hpy99I CGWCG 1 cut(s) 34
HpyCH4III ACNGT 1 cut(s) 170
HpyCH4IV ACGT 4 cut(s) 219, 230, 426, 483
HpyCH4V TGCA 6 cut(s) 174, 237, 260, 344, 501, 524
HpyF10VI GCNNNNNNNGC 2 cut(s) 512, 521
HpyF3I CTNAG 1 cut(s) 337
HpySE526I ACGT 4 cut(s) 219, 230, 426, 483
Kzo9I GATC 5 cut(s) 12, 130, 224, 390, 394
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 3 cut(s) 125, 159, 613
Lsp1109I GCAGC 2 cut(s) 221, 485
LweI GCATC 4 cut(s) 247, 339, 511, 603
MaeI CTAG 2 cut(s) 38, 80
MaeII ACGT 4 cut(s) 219, 230, 426, 483
MaeIII GTNAC 2 cut(s) 43, 439
MalI GATC 5 cut(s) 14, 132, 226, 392, 396
MboI GATC 5 cut(s) 12, 130, 224, 390, 394
MboII GAAGA 3 cut(s) 14, 299, 553
MflI RGATCY 2 cut(s) 12, 224
MhlI GDGCHC 1 cut(s) 346
MlsI TGGCCA 2 cut(s) 271, 535
MluCI AATT 1 cut(s) 365
MluNI TGGCCA 2 cut(s) 271, 535
MmeI TCCRAC 1 cut(s) 202
MnlI CCTC 6 cut(s) 167, 312, 315, 576, 579, 584
Mox20I TGGCCA 2 cut(s) 271, 535
MscI TGGCCA 2 cut(s) 271, 535
MseI TTAA 2 cut(s) 189, 200
MslI CAYNNNNRTG 1 cut(s) 619
Msp20I TGGCCA 2 cut(s) 271, 535
MspI CCGG 3 cut(s) 75, 117, 204
MspR9I CCNGG 4 cut(s) 75, 76, 117, 381
MvaI CCWGG 1 cut(s) 381
MwoI GCNNNNNNNGC 2 cut(s) 512, 521
NciI CCSGG 3 cut(s) 75, 76, 117
NdeII GATC 5 cut(s) 12, 130, 224, 390, 394
NheI GCTAGC 1 cut(s) 79
NlaIV GGNNCC 2 cut(s) 121, 226
NmuCI GTSAC 1 cut(s) 43
OliI CACNNNNGTG 1 cut(s) 619
PciSI GCTCTTC 1 cut(s) 27
PfeI GAWTC 2 cut(s) 34, 289
PinAI ACCGGT 1 cut(s) 203
PkrI GCNGC 2 cut(s) 236, 500
Ple19I CGATCG 1 cut(s) 393
PmaCI CACGTG 1 cut(s) 231
PmlI CACGTG 1 cut(s) 231
Ppu21I YACGTR 1 cut(s) 231
Psp1406I AACGTT 2 cut(s) 219, 426
Psp6I CCWGG 1 cut(s) 379
PspCI CACGTG 1 cut(s) 231
PspFI CCCAGC 1 cut(s) 101
PspGI CCWGG 1 cut(s) 379
PspN4I GGNNCC 2 cut(s) 121, 226
PsuI RGATCY 2 cut(s) 12, 224
PvuI CGATCG 1 cut(s) 393
RseI CAYNNNNRTG 1 cut(s) 619
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 2 cut(s) 189, 200
SatI GCNGC 2 cut(s) 235, 499
Sau3AI GATC 5 cut(s) 12, 130, 224, 390, 394
ScrFI CCNGG 4 cut(s) 75, 76, 117, 381
SduI GDGCHC 1 cut(s) 346
SetI ASST 9 cut(s) 10, 22, 85, 222, 233, 343, 429, 436, 486
SfaNI GCATC 4 cut(s) 247, 339, 511, 603
SmaI CCCGGG 1 cut(s) 76
SmiMI CAYNNNNRTG 1 cut(s) 619
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
SrfI GCCCGGGC 1 cut(s) 76
Sse9I AATT 1 cut(s) 365
SsiI CCGC 1 cut(s) 362
SspMI CTAG 2 cut(s) 38, 80
StyD4I CCNGG 4 cut(s) 73, 74, 115, 379
TaaI ACNGT 1 cut(s) 170
TaiI ACGT 4 cut(s) 222, 233, 429, 486
TaqI TCGA 3 cut(s) 29, 180, 393
TasI AATT 1 cut(s) 365
TfiI GAWTC 2 cut(s) 34, 289
Tru1I TTAA 2 cut(s) 189, 200
Tru9I TTAA 2 cut(s) 189, 200
TscAI CASTG 1 cut(s) 103
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 2 cut(s) 234, 498
Tsp45I GTSAC 1 cut(s) 43
TspGWI ACGGA 2 cut(s) 273, 546
TspMI CCCGGG 1 cut(s) 74
TspRI CASTG 1 cut(s) 103
VneI GTGCAC 1 cut(s) 342
XmaI CCCGGG 1 cut(s) 74
XspI CTAG 2 cut(s) 38, 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.