MD09G1164100.v1.1

Egg cell-secreted protein 1.1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002420
Physical Location & Seq
Forward (+)
13436950 .. 13437381
432 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1164100.v1.1.491

Sequence Viewer

Length: 432 bp
ATGTCTAGACAAGCTCAAGCACTACTTTCACTACTCCTACTGGCAACATGTGCTGCAACCGGGCTGGCGGATGTGCCACCACCGCCCTTCCTGACCATCCCAAGCCTATTCCCGCCAGGGACCCCAAGCCTGCTGCCACCCGGCATTCCCGGCCTACTCCCTCCGGGGATTCCAGGGCTGTCTCCGCCGGGAATCCCGAGTGAGGTAGCAAAGTGCTGGTCTTCATTTCAGAACATTCCAGGGTGTGCATCGGAGATTTATACCACAATCTTGACTGGTAAATTTGCACTAGGCCCTGCTTGTTGCAAGGCCTTCGTCGAAGTTGATGAGACTTGCTTGCGGAAGTTGTTCCCACTGTTTCCTTCCATTCCTGCATTCCTCAAGAGCAGCTGTGCTGCTGCTGCTCCTAAACCAGCTGGATCAAAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

144

Amino Acids

14.65

Weight (kDa)

8.53

Isoelectric Point (pI)

61.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 71 - 121 4.1e-13 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 68, 83, 113, 185, 340
AclWI GGATC 1 cut(s) 427
AcsI RAATTY 1 cut(s) 281
AfiI CCNNNNNNNGG 1 cut(s) 202
AflIII ACRYGT 1 cut(s) 47
AjnI CCWGG 3 cut(s) 115, 172, 238
AluBI AGCT 3 cut(s) 14, 390, 416
AluI AGCT 3 cut(s) 14, 390, 416
Alw26I GTCTC 2 cut(s) 186, 323
AlwI GGATC 1 cut(s) 427
Ama87I CYCGRG 1 cut(s) 196
AoxI GGCC 3 cut(s) 151, 292, 309
ApeKI GCWGC 6 cut(s) 53, 133, 387, 395, 398, 401
ApoI RAATTY 1 cut(s) 281
ArsI GACNNNNNNTTYG 2 cut(s) 203, 235
Asp700I GAANNNNTTC 1 cut(s) 347
AspS9I GGNCC 2 cut(s) 120, 293
AsuC2I CCSGG 5 cut(s) 61, 141, 150, 165, 189
AvaI CYCGRG 1 cut(s) 196
AvaII GGWCC 1 cut(s) 120
BbsI GAAGAC 1 cut(s) 213
BbvI GCAGC 6 cut(s) 40, 120, 382, 385, 388, 399
BccI CCATC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BciT130I CCWGG 3 cut(s) 117, 174, 240
BcnI CCSGG 5 cut(s) 61, 141, 150, 165, 189
BcoDI GTCTC 2 cut(s) 186, 323
BfaI CTAG 2 cut(s) 6, 290
BisI GCNGC 6 cut(s) 54, 134, 388, 396, 399, 402
BlsI GCNGC 6 cut(s) 55, 135, 389, 397, 400, 403
Bme1390I CCNGG 8 cut(s) 61, 117, 141, 150, 165, 174, 189, 240
Bme18I GGWCC 1 cut(s) 120
BmeT110I CYCGRG 1 cut(s) 196
BmgT120I GGNCC 2 cut(s) 120, 293
BmiI GGNNCC 2 cut(s) 121, 122
BmrFI CCNGG 8 cut(s) 61, 117, 141, 150, 165, 174, 189, 240
BmsI GCATC 1 cut(s) 257
BpiI GAAGAC 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 365
BpuMI CCSGG 5 cut(s) 61, 141, 150, 165, 189
BsaJI CCNNGG 4 cut(s) 116, 164, 173, 239
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse1I ACTGG 2 cut(s) 45, 280
BseBI CCWGG 3 cut(s) 117, 174, 240
BseDI CCNNGG 4 cut(s) 116, 164, 173, 239
BseGI GGATG 2 cut(s) 76, 96
BseLI CCNNNNNNNGG 1 cut(s) 202
BseNI ACTGG 2 cut(s) 45, 280
BseXI GCAGC 6 cut(s) 40, 120, 382, 385, 388, 399
BshFI GGCC 3 cut(s) 153, 294, 311
BsiHKCI CYCGRG 1 cut(s) 196
BsiSI CCGG 5 cut(s) 60, 141, 150, 164, 188
BslFI GGGAC 1 cut(s) 133
BslI CCNNNNNNNGG 1 cut(s) 202
BsmAI GTCTC 2 cut(s) 186, 323
BsmFI GGGAC 1 cut(s) 133
BsmI GAATGC 2 cut(s) 144, 374
BsnI GGCC 3 cut(s) 153, 294, 311
BsoBI CYCGRG 1 cut(s) 196
Bsp143I GATC 1 cut(s) 419
BspACI CCGC 5 cut(s) 68, 83, 113, 185, 340
BspANI GGCC 3 cut(s) 153, 294, 311
BspLI GGNNCC 2 cut(s) 121, 122
BspPI GGATC 1 cut(s) 427
BsrI ACTGG 2 cut(s) 45, 280
BssECI CCNNGG 4 cut(s) 116, 164, 173, 239
BssMI GATC 1 cut(s) 419
Bst2UI CCWGG 3 cut(s) 117, 174, 240
Bst4CI ACNGT 1 cut(s) 357
BstAPI GCANNNNNTGC 1 cut(s) 50
BstC8I GCNNGC 3 cut(s) 66, 131, 338
BstF5I GGATG 2 cut(s) 76, 96
BstKTI GATC 1 cut(s) 422
BstMAI GTCTC 2 cut(s) 186, 323
BstMBI GATC 1 cut(s) 419
BstMWI GCNNNNNNNGC 5 cut(s) 50, 82, 150, 184, 401
BstNI CCWGG 3 cut(s) 117, 174, 240
BstNSI RCATGY 1 cut(s) 51
BstSCI CCNGG 8 cut(s) 59, 115, 139, 148, 163, 172, 187, 238
BstV1I GCAGC 6 cut(s) 40, 120, 382, 385, 388, 399
BstV2I GAAGAC 1 cut(s) 213
BsuRI GGCC 3 cut(s) 153, 294, 311
BtsCI GGATG 2 cut(s) 76, 96
BtsIMutI CAGTG 1 cut(s) 353
Cac8I GCNNGC 3 cut(s) 66, 131, 338
Cfr13I GGNCC 2 cut(s) 120, 293
CviAII CATG 1 cut(s) 48
DpnI GATC 1 cut(s) 421
DpnII GATC 1 cut(s) 419
EciI GGCGGA 2 cut(s) 83, 174
Eco147I AGGCCT 1 cut(s) 311
Eco47I GGWCC 1 cut(s) 120
Eco88I CYCGRG 1 cut(s) 196
EcoO109I RGGNCCY 2 cut(s) 120, 293
EcoRII CCWGG 3 cut(s) 115, 172, 238
FaeI CATG 1 cut(s) 51
FaiI YATR 2 cut(s) 49, 261
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FaqI GGGAC 1 cut(s) 133
FatI CATG 1 cut(s) 47
FauI CCCGC 1 cut(s) 120
Fnu4HI GCNGC 6 cut(s) 54, 134, 388, 396, 399, 402
FokI GGATG 2 cut(s) 83, 83
Fsp4HI GCNGC 6 cut(s) 54, 134, 388, 396, 399, 402
FspBI CTAG 2 cut(s) 6, 290
GluI GCNGC 6 cut(s) 54, 134, 388, 396, 399, 402
HaeIII GGCC 3 cut(s) 153, 294, 311
HapII CCGG 5 cut(s) 60, 141, 150, 164, 188
Hin1II CATG 1 cut(s) 51
HinfI GANTC 2 cut(s) 169, 192
HpaII CCGG 5 cut(s) 60, 141, 150, 164, 188
Hpy188I TCNGA 2 cut(s) 231, 253
Hpy188III TCNNGA 5 cut(s) 6, 91, 196, 271, 382
Hpy99I CGWCG 1 cut(s) 320
HpyAV CCTTC 3 cut(s) 97, 322, 372
HpyCH4III ACNGT 1 cut(s) 357
HpyCH4V TGCA 5 cut(s) 56, 248, 287, 306, 374
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 82, 150, 184, 401
Hsp92II CATG 1 cut(s) 51
KflI GGGWCCC 1 cut(s) 120
Kzo9I GATC 1 cut(s) 419
LmnI GCTCC 1 cut(s) 409
Lsp1109I GCAGC 6 cut(s) 40, 120, 382, 385, 388, 399
LweI GCATC 1 cut(s) 257
MaeI CTAG 2 cut(s) 6, 290
MalI GATC 1 cut(s) 421
MboI GATC 1 cut(s) 419
MboII GAAGA 1 cut(s) 213
MluCI AATT 1 cut(s) 281
MnlI CCTC 3 cut(s) 171, 196, 389
MroXI GAANNNNTTC 1 cut(s) 347
MspA1I CMGCKG 2 cut(s) 390, 416
MspI CCGG 5 cut(s) 60, 141, 150, 164, 188
MspR9I CCNGG 8 cut(s) 61, 117, 141, 150, 165, 174, 189, 240
Mva1269I GAATGC 2 cut(s) 144, 374
MvaI CCWGG 3 cut(s) 117, 174, 240
MwoI GCNNNNNNNGC 5 cut(s) 50, 82, 150, 184, 401
NciI CCSGG 5 cut(s) 61, 141, 150, 165, 189
NdeII GATC 1 cut(s) 419
NlaIII CATG 1 cut(s) 51
NlaIV GGNNCC 2 cut(s) 121, 122
NspI RCATGY 1 cut(s) 51
PceI AGGCCT 1 cut(s) 311
PciI ACATGT 1 cut(s) 47
PctI GAATGC 2 cut(s) 144, 374
PdmI GAANNNNTTC 1 cut(s) 347
PfeI GAWTC 2 cut(s) 169, 192
PkrI GCNGC 6 cut(s) 55, 135, 389, 397, 400, 403
PpuMI RGGWCCY 1 cut(s) 120
PscI ACATGT 1 cut(s) 47
Psp5II RGGWCCY 1 cut(s) 120
Psp6I CCWGG 3 cut(s) 115, 172, 238
PspGI CCWGG 3 cut(s) 115, 172, 238
PspN4I GGNNCC 2 cut(s) 121, 122
PspPI GGNCC 2 cut(s) 120, 293
PspPPI RGGWCCY 1 cut(s) 120
PvuII CAGCTG 2 cut(s) 390, 416
SatI GCNGC 6 cut(s) 54, 134, 388, 396, 399, 402
Sau3AI GATC 1 cut(s) 419
Sau96I GGNCC 2 cut(s) 120, 293
ScrFI CCNGG 8 cut(s) 61, 117, 141, 150, 165, 174, 189, 240
SetI ASST 4 cut(s) 16, 207, 392, 418
SfaNI GCATC 1 cut(s) 257
SinI GGWCC 1 cut(s) 120
SmlI CTYRAG 2 cut(s) 15, 380
SmoI CTYRAG 2 cut(s) 15, 380
Sse9I AATT 1 cut(s) 281
SseBI AGGCCT 1 cut(s) 311
SsiI CCGC 5 cut(s) 68, 83, 113, 185, 340
SspMI CTAG 2 cut(s) 6, 290
StuI AGGCCT 1 cut(s) 311
StyD4I CCNGG 8 cut(s) 59, 115, 139, 148, 163, 172, 187, 238
TaaI ACNGT 1 cut(s) 357
TaqI TCGA 1 cut(s) 318
TasI AATT 1 cut(s) 281
TfiI GAWTC 2 cut(s) 169, 192
TscAI CASTG 1 cut(s) 360
TseI GCWGC 6 cut(s) 53, 133, 387, 395, 398, 401
TspDTI ATGAA 1 cut(s) 213
TspRI CASTG 1 cut(s) 360
VpaK11BI GGWCC 1 cut(s) 120
XapI RAATTY 1 cut(s) 281
XbaI TCTAGA 1 cut(s) 5
XceI RCATGY 1 cut(s) 51
XmnI GAANNNNTTC 1 cut(s) 347
XspI CTAG 2 cut(s) 6, 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.