AT4G35165

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002420
Physical Location & Seq
Forward (+)
16735123 .. 16735883
761 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G35165.1

Sequence Viewer

Length: 363 bp
ATGGGAAACAATTCAAGAGCTATTTTCTCTTTATTCTTGGTTGTAGCCTTATGTGCTGCAATTTTAATCACCCCCGGAATAGCTCATGATGAACAACCACCGCCCCGCTCACAATTTCCACCGGATTTTCCTATTGATGTGGAAAAATGTTGGGCATCACTTTTCAATACTCAGGGATGCGTATTCGAACTTTTAAAGTCAGTTTTTTCTGGTCAGTTTGGAAATGTTGGAGTTGCATGTTGCAAAGCATTGTCGACCATAGATGCTAACTGTTGGCCTCATATGTTCCCGCTTAACCCGTTCTTTCCACCTCTGCTTAAGGACAACTGTGCGCACATCGTCCCCAATTCTCCCTCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

120

Amino Acids

13.08

Weight (kDa)

6.23

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 49 - 110 5.7e-15 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 333
AccBSI CCGCTC 1 cut(s) 108
AccI GTMKAC 1 cut(s) 254
AciI CCGC 3 cut(s) 101, 106, 290
AflII CTTAAG 1 cut(s) 317
AgsI TTSAA 2 cut(s) 15, 166
AluBI AGCT 2 cut(s) 20, 83
AluI AGCT 2 cut(s) 20, 83
AoxI GGCC 1 cut(s) 275
ApeKI GCWGC 1 cut(s) 56
Asp700I GAANNNNTTC 1 cut(s) 10
AspLEI GCGC 1 cut(s) 334
AsuC2I CCSGG 1 cut(s) 75
AsuHPI GGTGA 1 cut(s) 61
AsuII TTCGAA 1 cut(s) 186
BbvI GCAGC 1 cut(s) 43
BcnI CCSGG 1 cut(s) 75
BfrI CTTAAG 1 cut(s) 317
BisI GCNGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 58
Bme1390I CCNGG 1 cut(s) 75
BmrFI CCNGG 1 cut(s) 75
BmsI GCATC 3 cut(s) 164, 167, 253
Bpu14I TTCGAA 1 cut(s) 186
BpuMI CCSGG 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 73
BsaWI WCCGGW 1 cut(s) 121
BseDI CCNNGG 1 cut(s) 73
BseGI GGATG 1 cut(s) 182
BseMII CTCAG 1 cut(s) 185
BseXI GCAGC 1 cut(s) 43
BshFI GGCC 1 cut(s) 277
BsiSI CCGG 2 cut(s) 75, 122
BslFI GGGAC 1 cut(s) 326
BsmFI GGGAC 1 cut(s) 326
BsnI GGCC 1 cut(s) 277
Bsp119I TTCGAA 1 cut(s) 186
BspACI CCGC 3 cut(s) 101, 106, 290
BspANI GGCC 1 cut(s) 277
BspCNI CTCAG 1 cut(s) 184
BspHI TCATGA 1 cut(s) 85
BspT104I TTCGAA 1 cut(s) 186
BspTI CTTAAG 1 cut(s) 317
BsrBI CCGCTC 1 cut(s) 108
BssECI CCNNGG 1 cut(s) 73
Bst4CI ACNGT 2 cut(s) 272, 329
BstAFI CTTAAG 1 cut(s) 317
BstBI TTCGAA 1 cut(s) 186
BstDEI CTNAG 1 cut(s) 171
BstF5I GGATG 1 cut(s) 182
BstHHI GCGC 1 cut(s) 334
BstMWI GCNNNNNNNGC 1 cut(s) 53
BstNSI RCATGY 1 cut(s) 240
BstSCI CCNGG 1 cut(s) 73
BstV1I GCAGC 1 cut(s) 43
BsuRI GGCC 1 cut(s) 277
BtsCI GGATG 1 cut(s) 182
CciI TCATGA 1 cut(s) 85
CfoI GCGC 1 cut(s) 334
CviAII CATG 2 cut(s) 86, 237
CviJI RGCY 4 cut(s) 20, 47, 83, 277
CviKI_1 RGCY 4 cut(s) 20, 47, 83, 277
DdeI CTNAG 1 cut(s) 171
DraI TTTAAA 1 cut(s) 195
FaeI CATG 2 cut(s) 89, 240
FaiI YATR 6 cut(s) 52, 87, 238, 260, 282, 284
FaqI GGGAC 1 cut(s) 326
FatI CATG 2 cut(s) 85, 236
FauI CCCGC 2 cut(s) 113, 297
FauNDI CATATG 1 cut(s) 282
FblI GTMKAC 1 cut(s) 254
Fnu4HI GCNGC 1 cut(s) 57
FokI GGATG 1 cut(s) 189
Fsp4HI GCNGC 1 cut(s) 57
FspAI RTGCGCAY 1 cut(s) 333
FspI TGCGCA 1 cut(s) 333
GlaI GCGC 1 cut(s) 333
GluI GCNGC 1 cut(s) 57
HaeIII GGCC 1 cut(s) 277
HapII CCGG 2 cut(s) 75, 122
HhaI GCGC 1 cut(s) 334
Hin1II CATG 2 cut(s) 89, 240
Hin6I GCGC 1 cut(s) 332
HinP1I GCGC 1 cut(s) 332
HincII GTYRAC 1 cut(s) 255
HindII GTYRAC 1 cut(s) 255
HpaII CCGG 2 cut(s) 75, 122
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 1 cut(s) 255
Hpy188III TCNNGA 2 cut(s) 15, 86
Hpy8I GTNNAC 1 cut(s) 255
HpyCH4III ACNGT 2 cut(s) 272, 329
HpyCH4V TGCA 3 cut(s) 59, 236, 243
HpyF10VI GCNNNNNNNGC 1 cut(s) 53
HpyF3I CTNAG 1 cut(s) 171
Hsp92II CATG 2 cut(s) 89, 240
HspAI GCGC 1 cut(s) 332
LpnPI CCDG 4 cut(s) 88, 135, 158, 195
Lsp1109I GCAGC 1 cut(s) 43
LweI GCATC 3 cut(s) 164, 167, 253
MbiI CCGCTC 1 cut(s) 108
MluCI AATT 4 cut(s) 10, 60, 113, 346
MmeI TCCRAC 1 cut(s) 208
MnlI CCTC 2 cut(s) 288, 321
MroXI GAANNNNTTC 1 cut(s) 10
MseI TTAA 4 cut(s) 65, 194, 294, 318
MspCI CTTAAG 1 cut(s) 317
MspI CCGG 2 cut(s) 75, 122
MspR9I CCNGG 1 cut(s) 75
MwoI GCNNNNNNNGC 1 cut(s) 53
NciI CCSGG 1 cut(s) 75
NdeI CATATG 1 cut(s) 282
NlaIII CATG 2 cut(s) 89, 240
NsbI TGCGCA 1 cut(s) 333
NspI RCATGY 1 cut(s) 240
NspV TTCGAA 1 cut(s) 186
PagI TCATGA 1 cut(s) 85
PdmI GAANNNNTTC 1 cut(s) 10
PkrI GCNGC 1 cut(s) 58
SalI GTCGAC 1 cut(s) 253
SaqAI TTAA 4 cut(s) 65, 194, 294, 318
SatI GCNGC 1 cut(s) 57
ScrFI CCNGG 1 cut(s) 75
SetI ASST 3 cut(s) 22, 85, 313
SfaNI GCATC 3 cut(s) 164, 167, 253
SfuI TTCGAA 1 cut(s) 186
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
Sse9I AATT 4 cut(s) 10, 60, 113, 346
SsiI CCGC 3 cut(s) 101, 106, 290
StyD4I CCNGG 1 cut(s) 73
TaaI ACNGT 2 cut(s) 272, 329
TaqI TCGA 2 cut(s) 186, 254
TasI AATT 4 cut(s) 10, 60, 113, 346
Tru1I TTAA 4 cut(s) 65, 194, 294, 318
Tru9I TTAA 4 cut(s) 65, 194, 294, 318
TseI GCWGC 1 cut(s) 56
TspDTI ATGAA 1 cut(s) 105
Vha464I CTTAAG 1 cut(s) 317
XceI RCATGY 1 cut(s) 240
XmiI GTMKAC 1 cut(s) 254
XmnI GAANNNNTTC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.