pycom17g15680

Egg cell-secreted protein 1.1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002420
Physical Location & Seq
Reverse (-)
13698294 .. 13699910
1617 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g15680.1

Sequence Viewer

Length: 489 bp
ATGCCATTTCTCATGACCAAGTACCCGTCCCACATCGAAAGCTACGATGTAAAGTACAGTTTCTCTTTCATGAAAAGAGAAGTTCCAAGCTTCCAAATAGCCATGTCTAGACAAGCTCAAGCATTACTTTCACTTCTCTTATTGGCAACATGTGCAGCAACCGGGCTGGCAGATGCGCCACCACCGCCCTTCCCGACCATCCCAGGCCTATTCCCTCCTGGGATTCCAGAGCTATCTCCGCCTGGAACCCCAGGTGAGATAGCCAAGTGCTGGTCTTCACTTCAGAACGTTCCAGGGTGTGCATCGGAAATTTATACCACAATCTTGACTGGTAAATTTTCTCTAGGCGCTGCTTGTTGCAAGGCCTTGGTCGAAGTTGATGAGAATTGCTTGCGGAAGTTGTTCCCGTTGTTTCCTTCCATTCCTGCTTTGCTCAAGAGCAGCTGTGATACTGCTGCTGCTCCTAAAGCAGCTGGATCGAAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

163

Amino Acids

17.21

Weight (kDa)

8.16

Isoelectric Point (pI)

66.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 89 - 149 6.4e-14 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 270
AciI CCGC 3 cut(s) 185, 239, 394
AclI AACGTT 1 cut(s) 288
AclWI GGATC 1 cut(s) 484
AcsI RAATTY 2 cut(s) 309, 335
AcuI CTGAAG 1 cut(s) 266
AfaI GTAC 2 cut(s) 23, 56
AfiI CCNNNNNNNGG 1 cut(s) 270
AflIII ACRYGT 1 cut(s) 149
AjnI CCWGG 5 cut(s) 202, 217, 241, 250, 292
AluBI AGCT 6 cut(s) 42, 90, 116, 232, 444, 473
AluI AGCT 6 cut(s) 42, 90, 116, 232, 444, 473
AlwI GGATC 1 cut(s) 484
AoxI GGCC 2 cut(s) 205, 363
ApeKI GCWGC 6 cut(s) 155, 350, 441, 455, 458, 470
ApoI RAATTY 2 cut(s) 309, 335
Asp700I GAANNNNTTC 1 cut(s) 401
AspLEI GCGC 2 cut(s) 178, 350
AsuC2I CCSGG 1 cut(s) 163
AsuHPI GGTGA 1 cut(s) 266
BbsI GAAGAC 1 cut(s) 267
BbvI GCAGC 6 cut(s) 167, 337, 442, 445, 453, 482
BccI CCATC 1 cut(s) 206
BcgI CGANNNNNNTGC 1 cut(s) 459
BciT130I CCWGG 5 cut(s) 204, 219, 243, 252, 294
BcnI CCSGG 1 cut(s) 163
BfaI CTAG 2 cut(s) 108, 344
BfoI RGCGCY 1 cut(s) 351
BisI GCNGC 6 cut(s) 156, 351, 442, 456, 459, 471
BlsI GCNGC 6 cut(s) 157, 352, 443, 457, 460, 472
Bme1390I CCNGG 6 cut(s) 163, 204, 219, 243, 252, 294
BmiI GGNNCC 1 cut(s) 247
BmrFI CCNGG 6 cut(s) 163, 204, 219, 243, 252, 294
BmsI GCATC 2 cut(s) 163, 311
BpiI GAAGAC 1 cut(s) 267
BpuEI CTTGAG 2 cut(s) 102, 419
BpuMI CCSGG 1 cut(s) 163
BsaJI CCNNGG 5 cut(s) 202, 218, 250, 293, 366
Bsc4I CCNNNNNNNGG 1 cut(s) 270
Bse1I ACTGG 1 cut(s) 334
BseBI CCWGG 5 cut(s) 204, 219, 243, 252, 294
BseDI CCNNGG 5 cut(s) 202, 218, 250, 293, 366
BseGI GGATG 1 cut(s) 198
BseLI CCNNNNNNNGG 1 cut(s) 270
BseNI ACTGG 1 cut(s) 334
BseXI GCAGC 6 cut(s) 167, 337, 442, 445, 453, 482
BsgI GTGCAG 1 cut(s) 174
BshFI GGCC 2 cut(s) 207, 365
BsiSI CCGG 1 cut(s) 162
BslFI GGGAC 1 cut(s) 13
BslI CCNNNNNNNGG 1 cut(s) 270
BsmFI GGGAC 1 cut(s) 13
BsnI GGCC 2 cut(s) 207, 365
Bsp143I GATC 1 cut(s) 476
BspACI CCGC 3 cut(s) 185, 239, 394
BspANI GGCC 2 cut(s) 207, 365
BspHI TCATGA 2 cut(s) 12, 69
BspLI GGNNCC 1 cut(s) 247
BspPI GGATC 1 cut(s) 484
BsrI ACTGG 1 cut(s) 334
BssECI CCNNGG 5 cut(s) 202, 218, 250, 293, 366
BssMI GATC 1 cut(s) 476
BssT1I CCWWGG 1 cut(s) 366
Bst2UI CCWGG 5 cut(s) 204, 219, 243, 252, 294
Bst4CI ACNGT 1 cut(s) 59
BstAPI GCANNNNNTGC 1 cut(s) 152
BstC8I GCNNGC 2 cut(s) 168, 392
BstF5I GGATG 1 cut(s) 198
BstH2I RGCGCY 1 cut(s) 351
BstHHI GCGC 2 cut(s) 178, 350
BstKTI GATC 1 cut(s) 479
BstMBI GATC 1 cut(s) 476
BstMWI GCNNNNNNNGC 4 cut(s) 152, 184, 238, 467
BstNI CCWGG 5 cut(s) 204, 219, 243, 252, 294
BstNSI RCATGY 1 cut(s) 153
BstSCI CCNGG 6 cut(s) 161, 202, 217, 241, 250, 292
BstV1I GCAGC 6 cut(s) 167, 337, 442, 445, 453, 482
BstV2I GAAGAC 1 cut(s) 267
BsuRI GGCC 2 cut(s) 207, 365
BtsCI GGATG 1 cut(s) 198
Cac8I GCNNGC 2 cut(s) 168, 392
CciI TCATGA 2 cut(s) 12, 69
CfoI GCGC 2 cut(s) 178, 350
Csp6I GTAC 2 cut(s) 22, 55
CviAII CATG 4 cut(s) 13, 70, 103, 150
CviQI GTAC 2 cut(s) 22, 55
DpnI GATC 1 cut(s) 478
DpnII GATC 1 cut(s) 476
EciI GGCGGA 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 366
Eco147I AGGCCT 2 cut(s) 207, 365
Eco57I CTGAAG 1 cut(s) 266
EcoRII CCWGG 5 cut(s) 202, 217, 241, 250, 292
EcoT14I CCWWGG 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 4 cut(s) 16, 73, 106, 153
FaiI YATR 5 cut(s) 14, 71, 104, 151, 315
FalI AAGNNNNNCTT 2 cut(s) 111, 143
FaqI GGGAC 1 cut(s) 13
FatI CATG 4 cut(s) 12, 69, 102, 149
Fnu4HI GCNGC 6 cut(s) 156, 351, 442, 456, 459, 471
FokI GGATG 1 cut(s) 185
Fsp4HI GCNGC 6 cut(s) 156, 351, 442, 456, 459, 471
FspBI CTAG 2 cut(s) 108, 344
GlaI GCGC 2 cut(s) 177, 349
GluI GCNGC 6 cut(s) 156, 351, 442, 456, 459, 471
HaeII RGCGCY 1 cut(s) 351
HaeIII GGCC 2 cut(s) 207, 365
HapII CCGG 1 cut(s) 162
HhaI GCGC 2 cut(s) 178, 350
Hin1II CATG 4 cut(s) 16, 73, 106, 153
Hin6I GCGC 2 cut(s) 176, 348
HinP1I GCGC 2 cut(s) 176, 348
HindIII AAGCTT 1 cut(s) 88
HinfI GANTC 1 cut(s) 223
HpaII CCGG 1 cut(s) 162
HphI GGTGA 1 cut(s) 266
Hpy188I TCNGA 2 cut(s) 285, 307
Hpy188III TCNNGA 7 cut(s) 13, 70, 108, 193, 227, 325, 436
HpyAV CCTTC 2 cut(s) 199, 426
HpyCH4III ACNGT 1 cut(s) 59
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 3 cut(s) 155, 302, 360
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 184, 238, 467
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 4 cut(s) 16, 73, 106, 153
HspAI GCGC 2 cut(s) 176, 348
Kzo9I GATC 1 cut(s) 476
LmnI GCTCC 1 cut(s) 466
Lsp1109I GCAGC 6 cut(s) 167, 337, 442, 445, 453, 482
LweI GCATC 2 cut(s) 163, 311
MaeI CTAG 2 cut(s) 108, 344
MaeII ACGT 1 cut(s) 288
MalI GATC 1 cut(s) 478
MboI GATC 1 cut(s) 476
MboII GAAGA 1 cut(s) 267
MluCI AATT 3 cut(s) 309, 335, 385
MnlI CCTC 1 cut(s) 225
MroXI GAANNNNTTC 1 cut(s) 401
MspA1I CMGCKG 2 cut(s) 444, 473
MspI CCGG 1 cut(s) 162
MspR9I CCNGG 6 cut(s) 163, 204, 219, 243, 252, 294
MvaI CCWGG 5 cut(s) 204, 219, 243, 252, 294
MwoI GCNNNNNNNGC 4 cut(s) 152, 184, 238, 467
NciI CCSGG 1 cut(s) 163
NdeII GATC 1 cut(s) 476
NlaIII CATG 4 cut(s) 16, 73, 106, 153
NlaIV GGNNCC 1 cut(s) 247
NspI RCATGY 1 cut(s) 153
PagI TCATGA 2 cut(s) 12, 69
PceI AGGCCT 2 cut(s) 207, 365
PciI ACATGT 1 cut(s) 149
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PdmI GAANNNNTTC 1 cut(s) 401
PfeI GAWTC 1 cut(s) 223
PflMI CCANNNNNTGG 1 cut(s) 270
PkrI GCNGC 6 cut(s) 157, 352, 443, 457, 460, 472
PscI ACATGT 1 cut(s) 149
Psp1406I AACGTT 1 cut(s) 288
Psp6I CCWGG 5 cut(s) 202, 217, 241, 250, 292
PspGI CCWGG 5 cut(s) 202, 217, 241, 250, 292
PspN4I GGNNCC 1 cut(s) 247
PvuII CAGCTG 2 cut(s) 444, 473
RsaI GTAC 2 cut(s) 23, 56
RsaNI GTAC 2 cut(s) 22, 55
SatI GCNGC 6 cut(s) 156, 351, 442, 456, 459, 471
Sau3AI GATC 1 cut(s) 476
ScrFI CCNGG 6 cut(s) 163, 204, 219, 243, 252, 294
SetI ASST 8 cut(s) 44, 92, 118, 234, 256, 291, 446, 475
SfaNI GCATC 2 cut(s) 163, 311
SmlI CTYRAG 2 cut(s) 117, 434
SmoI CTYRAG 2 cut(s) 117, 434
Sse9I AATT 3 cut(s) 309, 335, 385
SseBI AGGCCT 2 cut(s) 207, 365
SsiI CCGC 3 cut(s) 185, 239, 394
SspMI CTAG 2 cut(s) 108, 344
StuI AGGCCT 2 cut(s) 207, 365
StyD4I CCNGG 6 cut(s) 161, 202, 217, 241, 250, 292
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 1 cut(s) 59
TaiI ACGT 1 cut(s) 291
TaqI TCGA 3 cut(s) 36, 372, 479
TasI AATT 3 cut(s) 309, 335, 385
TatI WGTACW 1 cut(s) 54
TfiI GAWTC 1 cut(s) 223
TseI GCWGC 6 cut(s) 155, 350, 441, 455, 458, 470
TspDTI ATGAA 2 cut(s) 58, 86
Van91I CCANNNNNTGG 1 cut(s) 270
XapI RAATTY 2 cut(s) 309, 335
XbaI TCTAGA 1 cut(s) 107
XceI RCATGY 1 cut(s) 153
XmnI GAANNNNTTC 1 cut(s) 401
XspI CTAG 2 cut(s) 108, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.