AT5G53905

Prolamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002420
Physical Location & Seq
Reverse (-)
21888148 .. 21889254
1107 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G53905.1

Sequence Viewer

Length: 507 bp
ATGGATGTGGAGGGGACAGCCGAGGGGCTTGTGACAACTCGATTTCCTTCCATTCCTGGTTCTCTAGTTGATCTCACAAAATGTTGGTCATCGCTCTTCAATGTTCAAGGTTGCAATATCGAAATTTTAAAATATGTTTTAACCGCTAAGTTTGAAAGCTGCAAAGCGTTTACGGAAGTGGATGCAAACTGTTGGCCAAAAATGTTTCCACTGAATCCATTTTTCCCTCCTCTTGTCAAGGATGGTTGCTCTCGCATCATCTCAAGTGCACCCGCACACACGACACCTCAACTCCCTGTCATCCCTGCACCGGATGCGTCCGAGGACGATCGTAGCCGTCAATCACGCTCTAGCCGCACGGTCATGGTTCATCAACCAGGGTTAAGGGCCTGTCTCTTGAGAAATCAAGGAAACAGAGCGATGTGGGAGAAATTAGGAGCTTATATGAAACGCATACATGGGTACTGGGCATGGGTATTAGTGAATATATGGACGATAGCCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

168

Amino Acids

18.85

Weight (kDa)

8.81

Isoelectric Point (pI)

63.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 27 - 84 2.6e-10 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 144, 273, 355
AcoI YGGCCR 1 cut(s) 194
AcsI RAATTY 1 cut(s) 123
AfaI GTAC 1 cut(s) 464
AfiI CCNNNNNNNGG 1 cut(s) 310
AgsI TTSAA 3 cut(s) 100, 107, 155
AjnI CCWGG 2 cut(s) 55, 376
AluBI AGCT 2 cut(s) 159, 440
AluI AGCT 2 cut(s) 159, 440
Alw21I GWGCWC 1 cut(s) 271
Alw26I GTCTC 1 cut(s) 398
Alw44I GTGCAC 1 cut(s) 267
AoxI GGCC 2 cut(s) 194, 387
ApaLI GTGCAC 1 cut(s) 267
ApeKI GCWGC 1 cut(s) 159
ApoI RAATTY 1 cut(s) 123
ArsI GACNNNNNNTTYG 2 cut(s) 71, 103
AspS9I GGNCC 1 cut(s) 387
BaeGI GKGCMC 1 cut(s) 271
BalI TGGCCA 1 cut(s) 196
Bbv12I GWGCWC 1 cut(s) 271
BbvI GCAGC 1 cut(s) 146
BccI CCATC 1 cut(s) 236
BceAI ACGGC 1 cut(s) 321
BciT130I CCWGG 2 cut(s) 57, 378
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 2 cut(s) 65, 351
BisI GCNGC 2 cut(s) 160, 355
BlsI GCNGC 2 cut(s) 161, 356
Bme1390I CCNGG 2 cut(s) 57, 378
BmgT120I GGNCC 1 cut(s) 387
BmrFI CCNGG 2 cut(s) 57, 378
BmrI ACTGGG 1 cut(s) 475
BmsI GCATC 3 cut(s) 172, 264, 304
BmuI ACTGGG 1 cut(s) 475
BpuEI CTTGAG 2 cut(s) 247, 418
BsaJI CCNNGG 3 cut(s) 21, 321, 377
BsaWI WCCGGW 1 cut(s) 310
BsaXI ACNNNNNCTCC 2 cut(s) 276, 306
Bsc4I CCNNNNNNNGG 1 cut(s) 310
Bse1I ACTGG 1 cut(s) 470
BseBI CCWGG 2 cut(s) 57, 378
BseDI CCNNGG 3 cut(s) 21, 321, 377
BseGI GGATG 5 cut(s) 10, 187, 247, 300, 319
BseLI CCNNNNNNNGG 1 cut(s) 310
BseNI ACTGG 1 cut(s) 470
BseRI GAGGAG 1 cut(s) 219
BseSI GKGCMC 1 cut(s) 271
BseXI GCAGC 1 cut(s) 146
BsgI GTGCAG 1 cut(s) 291
Bsh1285I CGRYCG 1 cut(s) 331
BshFI GGCC 2 cut(s) 196, 389
BsiEI CGRYCG 1 cut(s) 331
BsiHKAI GWGCWC 1 cut(s) 271
BsiSI CCGG 1 cut(s) 311
BslFI GGGAC 1 cut(s) 28
BslI CCNNNNNNNGG 1 cut(s) 310
BsmAI GTCTC 1 cut(s) 398
BsmFI GGGAC 1 cut(s) 28
BsnI GGCC 2 cut(s) 196, 389
Bsp1286I GDGCHC 1 cut(s) 271
Bsp143I GATC 2 cut(s) 70, 328
BspACI CCGC 3 cut(s) 144, 273, 355
BspANI GGCC 2 cut(s) 196, 389
BspQI GCTCTTC 1 cut(s) 101
BsrI ACTGG 1 cut(s) 470
BssECI CCNNGG 3 cut(s) 21, 321, 377
BssMI GATC 2 cut(s) 70, 328
Bst2UI CCWGG 2 cut(s) 57, 378
Bst4CI ACNGT 2 cut(s) 191, 361
Bst6I CTCTTC 1 cut(s) 101
BstAPI GCANNNNNTGC 1 cut(s) 314
BstDEI CTNAG 1 cut(s) 147
BstF5I GGATG 5 cut(s) 10, 187, 247, 300, 319
BstKTI GATC 2 cut(s) 73, 331
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 2 cut(s) 70, 328
BstMCI CGRYCG 1 cut(s) 331
BstMWI GCNNNNNNNGC 2 cut(s) 314, 354
BstNI CCWGG 2 cut(s) 57, 378
BstSCI CCNGG 2 cut(s) 55, 376
BstSLI GKGCMC 1 cut(s) 271
BstV1I GCAGC 1 cut(s) 146
BsuRI GGCC 2 cut(s) 196, 389
BtgZI GCGATG 2 cut(s) 75, 434
BtsCI GGATG 5 cut(s) 10, 187, 247, 300, 319
BtsIMutI CAGTG 1 cut(s) 209
Cfr13I GGNCC 1 cut(s) 387
CseI GACGC 1 cut(s) 306
Csp6I GTAC 1 cut(s) 463
CviAII CATG 3 cut(s) 364, 458, 471
CviJI RGCY 9 cut(s) 20, 28, 159, 196, 336, 354, 389, 440, 500
CviKI_1 RGCY 9 cut(s) 20, 28, 159, 196, 336, 354, 389, 440, 500
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 1 cut(s) 147
DpnI GATC 2 cut(s) 72, 330
DpnII GATC 2 cut(s) 70, 328
DraI TTTAAA 1 cut(s) 129
EaeI YGGCCR 1 cut(s) 194
Eam1104I CTCTTC 1 cut(s) 101
EarI CTCTTC 1 cut(s) 101
EcoO109I RGGNCCY 1 cut(s) 387
EcoRII CCWGG 2 cut(s) 55, 376
FaeI CATG 3 cut(s) 367, 461, 474
FaiI YATR 9 cut(s) 135, 365, 444, 446, 455, 459, 472, 488, 490
FaqI GGGAC 1 cut(s) 28
FatI CATG 3 cut(s) 363, 457, 470
FauI CCCGC 1 cut(s) 280
Fnu4HI GCNGC 2 cut(s) 160, 355
FokI GGATG 5 cut(s) 17, 194, 254, 287, 326
Fsp4HI GCNGC 2 cut(s) 160, 355
FspBI CTAG 2 cut(s) 65, 351
GluI GCNGC 2 cut(s) 160, 355
HaeIII GGCC 2 cut(s) 196, 389
HapII CCGG 1 cut(s) 311
HgaI GACGC 1 cut(s) 306
Hin1II CATG 3 cut(s) 367, 461, 474
HinfI GANTC 1 cut(s) 214
HpaII CCGG 1 cut(s) 311
Hpy166II GTNNAC 2 cut(s) 171, 269
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 1 cut(s) 397
Hpy8I GTNNAC 2 cut(s) 171, 269
HpyAV CCTTC 1 cut(s) 57
HpyCH4III ACNGT 2 cut(s) 191, 361
HpyCH4V TGCA 5 cut(s) 114, 162, 185, 269, 308
HpyF10VI GCNNNNNNNGC 2 cut(s) 314, 354
HpyF3I CTNAG 1 cut(s) 147
Hsp92II CATG 3 cut(s) 367, 461, 474
Kzo9I GATC 2 cut(s) 70, 328
LguI GCTCTTC 1 cut(s) 101
LmnI GCTCC 1 cut(s) 437
LpnPI CCDG 9 cut(s) 42, 69, 309, 318, 324, 363, 390, 403, 451
Lsp1109I GCAGC 1 cut(s) 146
LweI GCATC 3 cut(s) 172, 264, 304
MaeI CTAG 2 cut(s) 65, 351
MaeIII GTNAC 1 cut(s) 31
MalI GATC 2 cut(s) 72, 330
MboI GATC 2 cut(s) 70, 328
MboII GAAGA 1 cut(s) 88
MhlI GDGCHC 1 cut(s) 271
MlsI TGGCCA 1 cut(s) 196
MluCI AATT 2 cut(s) 123, 431
MluNI TGGCCA 1 cut(s) 196
MnlI CCTC 6 cut(s) 4, 16, 237, 240, 297, 316
Mox20I TGGCCA 1 cut(s) 196
MscI TGGCCA 1 cut(s) 196
MseI TTAA 4 cut(s) 128, 140, 383, 505
MslI CAYNNNNRTG 1 cut(s) 362
Msp20I TGGCCA 1 cut(s) 196
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 2 cut(s) 57, 378
MvaI CCWGG 2 cut(s) 57, 378
MwoI GCNNNNNNNGC 2 cut(s) 314, 354
NdeII GATC 2 cut(s) 70, 328
NlaIII CATG 3 cut(s) 367, 461, 474
NmeAIII GCCGAG 1 cut(s) 46
NmuCI GTSAC 1 cut(s) 31
PciSI GCTCTTC 1 cut(s) 101
PfeI GAWTC 1 cut(s) 214
PkrI GCNGC 2 cut(s) 161, 356
Ple19I CGATCG 1 cut(s) 331
Psp6I CCWGG 2 cut(s) 55, 376
PspGI CCWGG 2 cut(s) 55, 376
PspPI GGNCC 1 cut(s) 387
PvuI CGATCG 1 cut(s) 331
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
RseI CAYNNNNRTG 1 cut(s) 362
SapI GCTCTTC 1 cut(s) 101
SaqAI TTAA 4 cut(s) 128, 140, 383, 505
SatI GCNGC 2 cut(s) 160, 355
Sau3AI GATC 2 cut(s) 70, 328
Sau96I GGNCC 1 cut(s) 387
ScrFI CCNGG 2 cut(s) 57, 378
SduI GDGCHC 1 cut(s) 271
SetI ASST 4 cut(s) 112, 161, 289, 442
SfaNI GCATC 3 cut(s) 172, 264, 304
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 2 cut(s) 262, 397
SmoI CTYRAG 2 cut(s) 262, 397
Sse9I AATT 2 cut(s) 123, 431
SsiI CCGC 3 cut(s) 144, 273, 355
SspMI CTAG 2 cut(s) 65, 351
StyD4I CCNGG 2 cut(s) 55, 376
TaaI ACNGT 2 cut(s) 191, 361
TaqI TCGA 2 cut(s) 40, 120
TasI AATT 2 cut(s) 123, 431
TauI GCSGC 1 cut(s) 357
TfiI GAWTC 1 cut(s) 214
Tru1I TTAA 4 cut(s) 128, 140, 383, 505
Tru9I TTAA 4 cut(s) 128, 140, 383, 505
TscAI CASTG 1 cut(s) 216
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 1 cut(s) 159
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 2 cut(s) 359, 461
TspGWI ACGGA 1 cut(s) 188
TspRI CASTG 1 cut(s) 216
VneI GTGCAC 1 cut(s) 267
XapI RAATTY 1 cut(s) 123
XspI CTAG 2 cut(s) 65, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.