Rh2AG473400

Egg cell-secreted protein 1.1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002420
Physical Location & Seq
Reverse (-)
69253677 .. 69254054
378 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG473400.1

Sequence Viewer

Length: 378 bp
ATGTCTAGAGCAGCTCAAGTGATATTTTGCCTCGTGCTAATGGTAACATGCGCCGCAACTGGGCTAGCGCATCAGGCACCCCCACCACCACCTCCTCTCTTTAACATTCCAGGCCTATTGCCGCCAGGGTTCCCCGGCCTACTGCCGCCAACAAACCCAAATGACATAGCAAAGTGTTGGTCTTCACTTCGGAACATTCAAGGATGTGTATCGGAAATCTTTACCTCAATCTTTACTCACAAAATAGAAGTAGGCCCTGCCTGTTGTAAAGCTTTCCTTGCAATTGAAGAGAGTTGCTGGCCGAAAATGTTCCCCTTAACTCCTTTCTTTGCTCCATTGCTCAAGAACACTTGTGCTCAGAAGGCAGCTCTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

125

Amino Acids

13.4

Weight (kDa)

8.48

Isoelectric Point (pI)

71.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 58 - 118 1e-17 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 76
AciI CCGC 3 cut(s) 54, 122, 146
AcoI YGGCCR 1 cut(s) 299
AfiI CCNNNNNNNGG 1 cut(s) 60
AgsI TTSAA 2 cut(s) 200, 287
AjnI CCWGG 2 cut(s) 109, 124
AluBI AGCT 3 cut(s) 14, 272, 368
AluI AGCT 3 cut(s) 14, 272, 368
Alw21I GWGCWC 1 cut(s) 358
AoxI GGCC 4 cut(s) 112, 136, 253, 299
ApeKI GCWGC 2 cut(s) 11, 365
ArsI GACNNNNNNTTYG 2 cut(s) 164, 196
Asp700I GAANNNNTTC 1 cut(s) 308
AspLEI GCGC 2 cut(s) 53, 70
AspS9I GGNCC 1 cut(s) 254
AsuC2I CCSGG 1 cut(s) 135
AsuNHI GCTAGC 1 cut(s) 64
BanI GGYRCC 1 cut(s) 76
BauI CACGAG 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 358
BbvI GCAGC 1 cut(s) 23
BciT130I CCWGG 2 cut(s) 111, 126
BcnI CCSGG 1 cut(s) 135
BfaI CTAG 2 cut(s) 6, 65
BisI GCNGC 5 cut(s) 12, 54, 122, 146, 366
BlsI GCNGC 5 cut(s) 13, 55, 123, 147, 367
Bme1390I CCNGG 3 cut(s) 111, 126, 135
BmgT120I GGNCC 1 cut(s) 254
BmiI GGNNCC 2 cut(s) 78, 131
BmrFI CCNGG 3 cut(s) 111, 126, 135
BmrI ACTGGG 1 cut(s) 69
BmsI GCATC 1 cut(s) 79
BmtI GCTAGC 1 cut(s) 68
BmuI ACTGGG 1 cut(s) 69
BpiI GAAGAC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 326
BpuMI CCSGG 1 cut(s) 135
BsaBI GATNNNNATC 1 cut(s) 208
BsaJI CCNNGG 2 cut(s) 125, 133
Bsc4I CCNNNNNNNGG 1 cut(s) 60
Bse1I ACTGG 1 cut(s) 64
Bse3DI GCAATG 1 cut(s) 335
Bse8I GATNNNNATC 1 cut(s) 208
BseBI CCWGG 2 cut(s) 111, 126
BseDI CCNNGG 2 cut(s) 125, 133
BseGI GGATG 1 cut(s) 209
BseJI GATNNNNATC 1 cut(s) 208
BseLI CCNNNNNNNGG 1 cut(s) 60
BseMI GCAATG 1 cut(s) 335
BseMII CTCAG 1 cut(s) 371
BseNI ACTGG 1 cut(s) 64
BseRI GAGGAG 1 cut(s) 84
BseXI GCAGC 1 cut(s) 23
BshFI GGCC 4 cut(s) 114, 138, 255, 301
BshNI GGYRCC 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 358
BsiSI CCGG 1 cut(s) 135
BslI CCNNNNNNNGG 1 cut(s) 60
BsnI GGCC 4 cut(s) 114, 138, 255, 301
Bsp1286I GDGCHC 1 cut(s) 358
BspACI CCGC 3 cut(s) 54, 122, 146
BspANI GGCC 4 cut(s) 114, 138, 255, 301
BspCNI CTCAG 1 cut(s) 370
BspLI GGNNCC 2 cut(s) 78, 131
BspOI GCTAGC 1 cut(s) 68
BspT107I GGYRCC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 335
BsrI ACTGG 1 cut(s) 64
BssECI CCNNGG 2 cut(s) 125, 133
BssSI CACGAG 1 cut(s) 32
Bst2BI CACGAG 1 cut(s) 32
Bst2UI CCWGG 2 cut(s) 111, 126
Bst6I CTCTTC 1 cut(s) 282
BstC8I GCNNGC 2 cut(s) 66, 299
BstDEI CTNAG 1 cut(s) 357
BstF5I GGATG 1 cut(s) 209
BstHHI GCGC 2 cut(s) 53, 70
BstMWI GCNNNNNNNGC 3 cut(s) 74, 278, 362
BstNI CCWGG 2 cut(s) 111, 126
BstNSI RCATGY 1 cut(s) 51
BstSCI CCNGG 3 cut(s) 109, 124, 133
BstV1I GCAGC 1 cut(s) 23
BstV2I GAAGAC 1 cut(s) 174
BsuRI GGCC 4 cut(s) 114, 138, 255, 301
BtsCI GGATG 1 cut(s) 209
Cac8I GCNNGC 2 cut(s) 66, 299
CfoI GCGC 2 cut(s) 53, 70
Cfr13I GGNCC 1 cut(s) 254
CviAII CATG 1 cut(s) 48
CviJI RGCY 8 cut(s) 14, 64, 114, 138, 255, 272, 301, 368
CviKI_1 RGCY 8 cut(s) 14, 64, 114, 138, 255, 272, 301, 368
DdeI CTNAG 1 cut(s) 357
EaeI YGGCCR 1 cut(s) 299
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
Eco147I AGGCCT 1 cut(s) 114
EcoO109I RGGNCCY 1 cut(s) 254
EcoRII CCWGG 2 cut(s) 109, 124
FaeI CATG 1 cut(s) 51
FaiI YATR 2 cut(s) 49, 167
FalI AAGNNNNNCTT 2 cut(s) 261, 293
FatI CATG 1 cut(s) 47
Fnu4HI GCNGC 5 cut(s) 12, 54, 122, 146, 366
FokI GGATG 1 cut(s) 216
Fsp4HI GCNGC 5 cut(s) 12, 54, 122, 146, 366
FspBI CTAG 2 cut(s) 6, 65
GlaI GCGC 2 cut(s) 52, 69
GluI GCNGC 5 cut(s) 12, 54, 122, 146, 366
HaeIII GGCC 4 cut(s) 114, 138, 255, 301
HapII CCGG 1 cut(s) 135
HhaI GCGC 2 cut(s) 53, 70
Hin1II CATG 1 cut(s) 51
Hin6I GCGC 2 cut(s) 51, 68
HinP1I GCGC 2 cut(s) 51, 68
HindIII AAGCTT 1 cut(s) 270
HpaII CCGG 1 cut(s) 135
Hpy188I TCNGA 3 cut(s) 192, 214, 360
Hpy188III TCNNGA 2 cut(s) 6, 343
HpyAV CCTTC 1 cut(s) 355
HpyCH4V TGCA 1 cut(s) 281
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 278, 362
HpyF3I CTNAG 1 cut(s) 357
Hsp92II CATG 1 cut(s) 51
HspAI GCGC 2 cut(s) 51, 68
LmnI GCTCC 1 cut(s) 337
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 1 cut(s) 79
MaeI CTAG 2 cut(s) 6, 65
MaeIII GTNAC 1 cut(s) 43
MboII GAAGA 2 cut(s) 174, 299
MfeI CAATTG 1 cut(s) 282
MhlI GDGCHC 1 cut(s) 358
MluCI AATT 1 cut(s) 282
MnlI CCTC 4 cut(s) 41, 102, 105, 235
MroXI GAANNNNTTC 1 cut(s) 308
MseI TTAA 3 cut(s) 102, 317, 376
MspI CCGG 1 cut(s) 135
MspR9I CCNGG 3 cut(s) 111, 126, 135
MunI CAATTG 1 cut(s) 282
MvaI CCWGG 2 cut(s) 111, 126
MwoI GCNNNNNNNGC 3 cut(s) 74, 278, 362
NciI CCSGG 1 cut(s) 135
NheI GCTAGC 1 cut(s) 64
NlaIII CATG 1 cut(s) 51
NlaIV GGNNCC 2 cut(s) 78, 131
NspI RCATGY 1 cut(s) 51
PceI AGGCCT 1 cut(s) 114
PdmI GAANNNNTTC 1 cut(s) 308
PkrI GCNGC 5 cut(s) 13, 55, 123, 147, 367
Psp6I CCWGG 2 cut(s) 109, 124
PspGI CCWGG 2 cut(s) 109, 124
PspN4I GGNNCC 2 cut(s) 78, 131
PspPI GGNCC 1 cut(s) 254
SaqAI TTAA 3 cut(s) 102, 317, 376
SatI GCNGC 5 cut(s) 12, 54, 122, 146, 366
Sau96I GGNCC 1 cut(s) 254
ScrFI CCNGG 3 cut(s) 111, 126, 135
SduI GDGCHC 1 cut(s) 358
SetI ASST 5 cut(s) 16, 94, 227, 274, 370
SfaNI GCATC 1 cut(s) 79
SmlI CTYRAG 2 cut(s) 15, 341
SmoI CTYRAG 2 cut(s) 15, 341
Sse9I AATT 1 cut(s) 282
SseBI AGGCCT 1 cut(s) 114
SsiI CCGC 3 cut(s) 54, 122, 146
SspMI CTAG 2 cut(s) 6, 65
StuI AGGCCT 1 cut(s) 114
StyD4I CCNGG 3 cut(s) 109, 124, 133
TasI AATT 1 cut(s) 282
TauI GCSGC 3 cut(s) 56, 124, 148
Tru1I TTAA 3 cut(s) 102, 317, 376
Tru9I TTAA 3 cut(s) 102, 317, 376
TseI GCWGC 2 cut(s) 11, 365
XbaI TCTAGA 1 cut(s) 5
XceI RCATGY 1 cut(s) 51
XmnI GAANNNNTTC 1 cut(s) 308
XspI CTAG 2 cut(s) 6, 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.