AT5G53742

Egg cell-secreted protein 1.1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002420
Physical Location & Seq
Forward (+)
21813671 .. 21814018
348 bp
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UTR
Exon/CDS
Intron
AT5G53742.1

Sequence Viewer

Length: 348 bp
ATGTTTCCGTTGAATCCATTATTCCCTCCTCTTCTCAAGGATGGTTGCTCTCGCATCATCTCTGGCGCACCAACACACACGAAACCTCAATTCCTTGTCATCCCTGGTTCTCCGATCGATCTCACAAAATGTTTGTCATCACTTGTTAACGTTGAAGGTTGCGTAACTGAAATCTACAAATCAGTTTTCACAGGAAAGTTTGGTAATGTTGGATATATGTGCTGCAAGGCGTTTTCAGCTGTGGATGTAAAATGTTGGCCACAAATGTTTCCGCTAAATCCGTTCTTCCCTCCTCTTCTCAAGAAGGAATGTTCTCCTATCAACGCAGCCACTCCTACACACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

115

Amino Acids

12.57

Weight (kDa)

8.91

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 43 - 105 5.8e-15 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 272
AclI AACGTT 1 cut(s) 150
AcoI YGGCCR 1 cut(s) 257
AgsI TTSAA 2 cut(s) 13, 155
AjnI CCWGG 1 cut(s) 103
AluBI AGCT 1 cut(s) 239
AluI AGCT 1 cut(s) 239
AoxI GGCC 1 cut(s) 257
ApeKI GCWGC 2 cut(s) 222, 326
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
AspLEI GCGC 1 cut(s) 68
BalI TGGCCA 1 cut(s) 259
BbvI GCAGC 2 cut(s) 209, 338
BccI CCATC 1 cut(s) 35
BciT130I CCWGG 1 cut(s) 105
BisI GCNGC 2 cut(s) 223, 327
BlsI GCNGC 2 cut(s) 224, 328
Bme1390I CCNGG 1 cut(s) 105
BmrFI CCNGG 1 cut(s) 105
BmsI GCATC 1 cut(s) 63
BpuEI CTTGAG 2 cut(s) 20, 284
Bsa29I ATCGAT 1 cut(s) 117
BsaJI CCNNGG 1 cut(s) 103
BseBI CCWGG 1 cut(s) 105
BseCI ATCGAT 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 103
BseGI GGATG 3 cut(s) 46, 99, 250
BseRI GAGGAG 2 cut(s) 18, 282
BseXI GCAGC 2 cut(s) 209, 338
Bsh1285I CGRYCG 1 cut(s) 117
BshFI GGCC 1 cut(s) 259
BshVI ATCGAT 1 cut(s) 117
BsiEI CGRYCG 1 cut(s) 117
BsnI GGCC 1 cut(s) 259
Bsp143I GATC 2 cut(s) 114, 118
BspACI CCGC 1 cut(s) 272
BspANI GGCC 1 cut(s) 259
BspDI ATCGAT 1 cut(s) 117
BssECI CCNNGG 1 cut(s) 103
BssMI GATC 2 cut(s) 114, 118
Bst2UI CCWGG 1 cut(s) 105
Bst6I CTCTTC 2 cut(s) 36, 300
BstF5I GGATG 3 cut(s) 46, 99, 250
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 2 cut(s) 117, 121
BstMBI GATC 2 cut(s) 114, 118
BstMCI CGRYCG 1 cut(s) 117
BstMWI GCNNNNNNNGC 1 cut(s) 236
BstNI CCWGG 1 cut(s) 105
BstSCI CCNGG 1 cut(s) 103
BstV1I GCAGC 2 cut(s) 209, 338
Bsu15I ATCGAT 1 cut(s) 117
BsuRI GGCC 1 cut(s) 259
BsuTUI ATCGAT 1 cut(s) 117
BtsCI GGATG 3 cut(s) 46, 99, 250
CfoI GCGC 1 cut(s) 68
ClaI ATCGAT 1 cut(s) 117
CviJI RGCY 3 cut(s) 239, 259, 329
CviKI_1 RGCY 3 cut(s) 239, 259, 329
DpnI GATC 2 cut(s) 116, 120
DpnII GATC 2 cut(s) 114, 118
EaeI YGGCCR 1 cut(s) 257
Eam1104I CTCTTC 2 cut(s) 36, 300
EarI CTCTTC 2 cut(s) 36, 300
EcoRII CCWGG 1 cut(s) 103
FaiI YATR 2 cut(s) 216, 218
Fnu4HI GCNGC 2 cut(s) 223, 327
FokI GGATG 3 cut(s) 53, 86, 257
Fsp4HI GCNGC 2 cut(s) 223, 327
GlaI GCGC 1 cut(s) 67
GluI GCNGC 2 cut(s) 223, 327
HaeIII GGCC 1 cut(s) 259
HhaI GCGC 1 cut(s) 68
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 1 cut(s) 13
HpaI GTTAAC 1 cut(s) 148
Hpy166II GTNNAC 1 cut(s) 148
Hpy188I TCNGA 1 cut(s) 114
Hpy188III TCNNGA 1 cut(s) 301
Hpy8I GTNNAC 1 cut(s) 148
HpyAV CCTTC 2 cut(s) 149, 298
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 1 cut(s) 225
HpyF10VI GCNNNNNNNGC 1 cut(s) 236
HpySE526I ACGT 1 cut(s) 150
HspAI GCGC 1 cut(s) 66
KspAI GTTAAC 1 cut(s) 148
Kzo9I GATC 2 cut(s) 114, 118
LpnPI CCDG 4 cut(s) 48, 90, 117, 177
Lsp1109I GCAGC 2 cut(s) 209, 338
LweI GCATC 1 cut(s) 63
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 163
MalI GATC 2 cut(s) 116, 120
MboI GATC 2 cut(s) 114, 118
MboII GAAGA 3 cut(s) 23, 277, 287
MlsI TGGCCA 1 cut(s) 259
MluCI AATT 1 cut(s) 89
MluNI TGGCCA 1 cut(s) 259
MmeI TCCRAC 1 cut(s) 190
MnlI CCTC 5 cut(s) 36, 39, 96, 300, 303
Mox20I TGGCCA 1 cut(s) 259
MscI TGGCCA 1 cut(s) 259
MseI TTAA 1 cut(s) 147
MslI CAYNNNNRTG 1 cut(s) 343
Msp20I TGGCCA 1 cut(s) 259
MspA1I CMGCKG 1 cut(s) 239
MspR9I CCNGG 1 cut(s) 105
MvaI CCWGG 1 cut(s) 105
MwoI GCNNNNNNNGC 1 cut(s) 236
NdeII GATC 2 cut(s) 114, 118
PfeI GAWTC 1 cut(s) 13
PkrI GCNGC 2 cut(s) 224, 328
Ple19I CGATCG 1 cut(s) 117
Psp1406I AACGTT 1 cut(s) 150
Psp6I CCWGG 1 cut(s) 103
PspGI CCWGG 1 cut(s) 103
PvuI CGATCG 1 cut(s) 117
PvuII CAGCTG 1 cut(s) 239
RseI CAYNNNNRTG 1 cut(s) 343
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 2 cut(s) 223, 327
Sau3AI GATC 2 cut(s) 114, 118
ScrFI CCNGG 1 cut(s) 105
SetI ASST 4 cut(s) 88, 153, 160, 241
SfaNI GCATC 1 cut(s) 63
SmiMI CAYNNNNRTG 1 cut(s) 343
SmlI CTYRAG 2 cut(s) 35, 299
SmoI CTYRAG 2 cut(s) 35, 299
Sse9I AATT 1 cut(s) 89
SsiI CCGC 1 cut(s) 272
StyD4I CCNGG 1 cut(s) 103
TaiI ACGT 1 cut(s) 153
TaqI TCGA 1 cut(s) 117
TasI AATT 1 cut(s) 89
TfiI GAWTC 1 cut(s) 13
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TseI GCWGC 2 cut(s) 222, 326
TspGWI ACGGA 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.