MD02G1018800.v1.1

Egg cell-secreted protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002420
Physical Location & Seq
Forward (+)
1373601 .. 1374011
411 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1018800.v1.1.491

Sequence Viewer

Length: 411 bp
ATGGCGGCAAAATCCCCAGCAAAATACTCAATGCTGTTCTTGCTCGCGGCTTGTGCGGCAAGCATAGGCGTAGCACTGTCGACCGCACCGCTGGCAGCATCACCACTACCCATCTTGCCGCACGGCCCCTGGGAGTCGGGACTTCCAAGGACAAGGACATTTCCAGGTCGGCCCGGCTGGAAAGAGCTCCGACAGTGCTGGGCTCCTCTCCAACATGTAACAGGCTGCGTCGACCAGATTTACGATGCGCTCTCCAACGCCCGATTAGACCTGGTCGGGCCGGCTTGTTGTAAGGCCGTCGCTGAAACGTTTGACGATTGTTGGGCCAAGCTGTTTCCTTTCAACCCATCTTTCCCTCCATCTCTTAAGAGCTTTTGCACCGCTCATCCACCTTCACCCAGGTCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

137

Amino Acids

14.58

Weight (kDa)

8.77

Isoelectric Point (pI)

62.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 65 - 126 9.6e-12 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 383
AccI GTMKAC 2 cut(s) 80, 231
AccII CGCG 1 cut(s) 47
AciI CCGC 7 cut(s) 5, 47, 56, 84, 89, 119, 381
AclI AACGTT 1 cut(s) 308
AflII CTTAAG 1 cut(s) 365
AflIII ACRYGT 1 cut(s) 214
AgsI TTSAA 1 cut(s) 343
AjnI CCWGG 4 cut(s) 128, 163, 270, 398
AluBI AGCT 3 cut(s) 187, 331, 372
AluI AGCT 3 cut(s) 187, 331, 372
Alw21I GWGCWC 1 cut(s) 189
AoxI GGCC 5 cut(s) 124, 170, 278, 294, 324
ApeKI GCWGC 2 cut(s) 95, 225
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 4 cut(s) 125, 171, 278, 324
AsuC2I CCSGG 1 cut(s) 174
AsuHPI GGTGA 2 cut(s) 93, 387
BanII GRGCYC 2 cut(s) 189, 205
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 107, 212
BccI CCATC 3 cut(s) 119, 355, 367
BceAI ACGGC 2 cut(s) 139, 281
BciT130I CCWGG 4 cut(s) 130, 165, 272, 400
BcnI CCSGG 1 cut(s) 174
BfrI CTTAAG 1 cut(s) 365
BisI GCNGC 6 cut(s) 6, 48, 57, 96, 119, 226
BlsI GCNGC 6 cut(s) 7, 49, 58, 97, 120, 227
Bme1390I CCNGG 5 cut(s) 130, 165, 174, 272, 400
BmgT120I GGNCC 4 cut(s) 125, 171, 278, 324
BmiI GGNNCC 2 cut(s) 127, 204
BmrFI CCNGG 5 cut(s) 130, 165, 174, 272, 400
BmsI GCATC 2 cut(s) 107, 235
BpuMI CCSGG 1 cut(s) 174
BsaJI CCNNGG 4 cut(s) 128, 129, 146, 398
Bse118I RCCGGY 1 cut(s) 280
BseBI CCWGG 4 cut(s) 130, 165, 272, 400
BseDI CCNNGG 4 cut(s) 128, 129, 146, 398
BseGI GGATG 1 cut(s) 385
BseRI GAGGAG 1 cut(s) 195
BseXI GCAGC 2 cut(s) 107, 212
BseYI CCCAGC 2 cut(s) 16, 198
Bsh1236I CGCG 1 cut(s) 47
Bsh1285I CGRYCG 1 cut(s) 84
BshFI GGCC 5 cut(s) 126, 172, 280, 296, 326
BsiEI CGRYCG 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 189
BsiSI CCGG 2 cut(s) 174, 281
BslFI GGGAC 1 cut(s) 153
BsmFI GGGAC 1 cut(s) 153
BsnI GGCC 5 cut(s) 126, 172, 280, 296, 326
Bsp1286I GDGCHC 2 cut(s) 189, 205
BspACI CCGC 7 cut(s) 5, 47, 56, 84, 89, 119, 381
BspANI GGCC 5 cut(s) 126, 172, 280, 296, 326
BspFNI CGCG 1 cut(s) 47
BspLI GGNNCC 2 cut(s) 127, 204
BspTI CTTAAG 1 cut(s) 365
BsrBI CCGCTC 1 cut(s) 383
BsrFI RCCGGY 1 cut(s) 280
BssAI RCCGGY 1 cut(s) 280
BssECI CCNNGG 4 cut(s) 128, 129, 146, 398
BssT1I CCWWGG 1 cut(s) 146
Bst2UI CCWGG 4 cut(s) 130, 165, 272, 400
Bst4CI ACNGT 2 cut(s) 78, 195
BstAFI CTTAAG 1 cut(s) 365
BstC8I GCNNGC 4 cut(s) 45, 61, 93, 282
BstF5I GGATG 1 cut(s) 385
BstFNI CGCG 1 cut(s) 47
BstHHI GCGC 1 cut(s) 250
BstMCI CGRYCG 1 cut(s) 84
BstMWI GCNNNNNNNGC 4 cut(s) 40, 53, 56, 92
BstNI CCWGG 4 cut(s) 130, 165, 272, 400
BstNSI RCATGY 1 cut(s) 218
BstSCI CCNGG 5 cut(s) 128, 163, 172, 270, 398
BstUI CGCG 1 cut(s) 47
BstV1I GCAGC 2 cut(s) 107, 212
BsuRI GGCC 5 cut(s) 126, 172, 280, 296, 326
BtsCI GGATG 1 cut(s) 385
BtsIMutI CAGTG 2 cut(s) 74, 200
Cac8I GCNNGC 4 cut(s) 45, 61, 93, 282
CfoI GCGC 1 cut(s) 250
Cfr10I RCCGGY 1 cut(s) 280
Cfr13I GGNCC 4 cut(s) 125, 171, 278, 324
CseI GACGC 1 cut(s) 217
CsiI ACCWGGT 1 cut(s) 270
CviAII CATG 1 cut(s) 215
Ecl136II GAGCTC 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 146
Eco24I GRGCYC 2 cut(s) 189, 205
Eco53kI GAGCTC 1 cut(s) 187
EcoICRI GAGCTC 1 cut(s) 187
EcoRII CCWGG 4 cut(s) 128, 163, 270, 398
EcoT14I CCWWGG 1 cut(s) 146
EcoT38I GRGCYC 2 cut(s) 189, 205
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 1 cut(s) 218
FaiI YATR 2 cut(s) 65, 216
FaqI GGGAC 1 cut(s) 153
FatI CATG 1 cut(s) 214
FblI GTMKAC 2 cut(s) 80, 231
Fnu4HI GCNGC 6 cut(s) 6, 48, 57, 96, 119, 226
FokI GGATG 1 cut(s) 372
FriOI GRGCYC 2 cut(s) 189, 205
Fsp4HI GCNGC 6 cut(s) 6, 48, 57, 96, 119, 226
GlaI GCGC 1 cut(s) 249
GluI GCNGC 6 cut(s) 6, 48, 57, 96, 119, 226
GsaI CCCAGC 2 cut(s) 20, 202
HaeIII GGCC 5 cut(s) 126, 172, 280, 296, 326
HapII CCGG 2 cut(s) 174, 281
HgaI GACGC 1 cut(s) 217
HhaI GCGC 1 cut(s) 250
Hin1II CATG 1 cut(s) 218
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HincII GTYRAC 3 cut(s) 81, 232, 405
HindII GTYRAC 3 cut(s) 81, 232, 405
HinfI GANTC 1 cut(s) 134
HpaII CCGG 2 cut(s) 174, 281
HphI GGTGA 2 cut(s) 93, 387
Hpy166II GTNNAC 3 cut(s) 81, 232, 405
Hpy188I TCNGA 1 cut(s) 191
Hpy188III TCNNGA 1 cut(s) 138
Hpy8I GTNNAC 3 cut(s) 81, 232, 405
Hpy99I CGWCG 2 cut(s) 233, 302
HpyAV CCTTC 1 cut(s) 402
HpyCH4III ACNGT 2 cut(s) 78, 195
HpyCH4IV ACGT 1 cut(s) 308
HpyCH4V TGCA 1 cut(s) 378
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 53, 56, 92
HpySE526I ACGT 1 cut(s) 308
Hsp92II CATG 1 cut(s) 218
HspAI GCGC 1 cut(s) 248
KroI GCCGGC 1 cut(s) 280
KroNI GCCGGC 1 cut(s) 282
LmnI GCTCC 2 cut(s) 192, 208
Lsp1109I GCAGC 2 cut(s) 107, 212
LweI GCATC 2 cut(s) 107, 235
MabI ACCWGGT 1 cut(s) 270
MaeII ACGT 1 cut(s) 308
MaeIII GTNAC 1 cut(s) 217
MbiI CCGCTC 1 cut(s) 383
MhlI GDGCHC 2 cut(s) 189, 205
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 3 cut(s) 214, 235, 279
MnlI CCTC 2 cut(s) 216, 366
MroNI GCCGGC 1 cut(s) 280
MseI TTAA 1 cut(s) 366
MspA1I CMGCKG 1 cut(s) 91
MspCI CTTAAG 1 cut(s) 365
MspI CCGG 2 cut(s) 174, 281
MspR9I CCNGG 5 cut(s) 130, 165, 174, 272, 400
MvaI CCWGG 4 cut(s) 130, 165, 272, 400
MvnI CGCG 1 cut(s) 47
MwoI GCNNNNNNNGC 4 cut(s) 40, 53, 56, 92
NaeI GCCGGC 1 cut(s) 282
NciI CCSGG 1 cut(s) 174
NgoMIV GCCGGC 1 cut(s) 280
NlaIII CATG 1 cut(s) 218
NlaIV GGNNCC 2 cut(s) 127, 204
NspI RCATGY 1 cut(s) 218
PasI CCCWGGG 1 cut(s) 129
PciI ACATGT 1 cut(s) 214
PdiI GCCGGC 1 cut(s) 282
PflFI GACNNNGTC 1 cut(s) 272
PkrI GCNGC 6 cut(s) 7, 49, 58, 97, 120, 227
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
PscI ACATGT 1 cut(s) 214
Psp124BI GAGCTC 1 cut(s) 189
Psp1406I AACGTT 1 cut(s) 308
Psp6I CCWGG 4 cut(s) 128, 163, 270, 398
PspFI CCCAGC 2 cut(s) 16, 198
PspGI CCWGG 4 cut(s) 128, 163, 270, 398
PspN4I GGNNCC 2 cut(s) 127, 204
PspPI GGNCC 4 cut(s) 125, 171, 278, 324
PsyI GACNNNGTC 1 cut(s) 272
SacI GAGCTC 1 cut(s) 189
SalI GTCGAC 2 cut(s) 79, 230
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 6 cut(s) 6, 48, 57, 96, 119, 226
Sau96I GGNCC 4 cut(s) 125, 171, 278, 324
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 5 cut(s) 130, 165, 174, 272, 400
SduI GDGCHC 2 cut(s) 189, 205
SetI ASST 8 cut(s) 169, 189, 273, 311, 333, 374, 394, 404
SexAI ACCWGGT 1 cut(s) 270
SfaNI GCATC 2 cut(s) 107, 235
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
SsiI CCGC 7 cut(s) 5, 47, 56, 84, 89, 119, 381
SstI GAGCTC 1 cut(s) 189
StyD4I CCNGG 5 cut(s) 128, 163, 172, 270, 398
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 2 cut(s) 78, 195
TaiI ACGT 1 cut(s) 311
TaqI TCGA 2 cut(s) 80, 231
TauI GCSGC 4 cut(s) 8, 50, 59, 121
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TscAI CASTG 2 cut(s) 81, 200
TseI GCWGC 2 cut(s) 95, 225
TspRI CASTG 2 cut(s) 81, 200
Tth111I GACNNNGTC 1 cut(s) 272
Vha464I CTTAAG 1 cut(s) 365
XceI RCATGY 1 cut(s) 218
XmiI GTMKAC 2 cut(s) 80, 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.