MD17G1163700.v1.1

Egg cell-secreted protein 1.1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002420
Physical Location & Seq
Reverse (-)
16204678 .. 16205109
432 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1163700.v1.1.491

Sequence Viewer

Length: 387 bp
ATGTCTAGACAAGCTCAAGCATTACTTTCACTTCTCCTATTGGCAACATGTGCAGCAACCGGGCTGGCAGATGCGCCACCACCGCCCTTCTCGACCATCCCAGGCCTATTCCCTCCTGGGATTCCAGGGCTATCTTCGCCTAGAACCCCAGGTGAGATAGCCAAGTGCTGGTCTTCACTTCAGAACGTTCCAGGGTGTGCATCGGAAATTTATCCCACAATCTTGACTGGTAAATTTGCTCTAGGCCCTGCTTGTTGCAAGGCCTTGGTCGAAGTTGATGAGAATTGCTTGCGGAAGTTGTTCCCGTTGTTTCCATCCATTCCTGCAGTGCTCAAGAGCAGCTGTACTACTGCTGCTGCTCCTAAAGCAGCTAGATCAAAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

129

Amino Acids

13.21

Weight (kDa)

8.96

Isoelectric Point (pI)

65.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 55 - 114 2.2e-13 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 168
AciI CCGC 2 cut(s) 83, 292
AclI AACGTT 1 cut(s) 186
AcsI RAATTY 2 cut(s) 207, 233
AcuI CTGAAG 1 cut(s) 164
AfaI GTAC 1 cut(s) 346
AfiI CCNNNNNNNGG 1 cut(s) 168
AflIII ACRYGT 1 cut(s) 47
AjnI CCWGG 5 cut(s) 100, 115, 124, 148, 190
AluBI AGCT 3 cut(s) 14, 342, 371
AluI AGCT 3 cut(s) 14, 342, 371
Alw21I GWGCWC 1 cut(s) 333
AoxI GGCC 3 cut(s) 103, 244, 261
ApeKI GCWGC 5 cut(s) 53, 339, 353, 356, 368
ApoI RAATTY 2 cut(s) 207, 233
Asp700I GAANNNNTTC 1 cut(s) 299
AspLEI GCGC 1 cut(s) 76
AspS9I GGNCC 1 cut(s) 245
AsuC2I CCSGG 1 cut(s) 61
AsuHPI GGTGA 1 cut(s) 164
BbsI GAAGAC 1 cut(s) 165
Bbv12I GWGCWC 1 cut(s) 333
BbvI GCAGC 5 cut(s) 65, 340, 343, 351, 380
BccI CCATC 2 cut(s) 104, 322
BciT130I CCWGG 5 cut(s) 102, 117, 126, 150, 192
BcnI CCSGG 1 cut(s) 61
BfaI CTAG 4 cut(s) 6, 141, 242, 372
BfmI CTRYAG 1 cut(s) 324
BisI GCNGC 5 cut(s) 54, 340, 354, 357, 369
BlsI GCNGC 5 cut(s) 55, 341, 355, 358, 370
Bme1390I CCNGG 6 cut(s) 61, 102, 117, 126, 150, 192
BmgT120I GGNCC 1 cut(s) 245
BmrFI CCNGG 6 cut(s) 61, 102, 117, 126, 150, 192
BmsI GCATC 2 cut(s) 61, 209
BpiI GAAGAC 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 317
BpuMI CCSGG 1 cut(s) 61
BsaJI CCNNGG 6 cut(s) 100, 116, 125, 148, 191, 264
Bsc4I CCNNNNNNNGG 1 cut(s) 168
Bse1I ACTGG 1 cut(s) 232
BseBI CCWGG 5 cut(s) 102, 117, 126, 150, 192
BseDI CCNNGG 6 cut(s) 100, 116, 125, 148, 191, 264
BseGI GGATG 2 cut(s) 96, 314
BseLI CCNNNNNNNGG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 232
BseXI GCAGC 5 cut(s) 65, 340, 343, 351, 380
BsgI GTGCAG 1 cut(s) 72
BshFI GGCC 3 cut(s) 105, 246, 263
BsiHKAI GWGCWC 1 cut(s) 333
BsiSI CCGG 1 cut(s) 60
BslI CCNNNNNNNGG 1 cut(s) 168
BsnI GGCC 3 cut(s) 105, 246, 263
Bsp1286I GDGCHC 1 cut(s) 333
Bsp143I GATC 1 cut(s) 374
BspACI CCGC 2 cut(s) 83, 292
BspANI GGCC 3 cut(s) 105, 246, 263
BspMAI CTGCAG 1 cut(s) 328
BsrI ACTGG 1 cut(s) 232
BssECI CCNNGG 6 cut(s) 100, 116, 125, 148, 191, 264
BssMI GATC 1 cut(s) 374
BssT1I CCWWGG 1 cut(s) 264
Bst2UI CCWGG 5 cut(s) 102, 117, 126, 150, 192
BstAPI GCANNNNNTGC 1 cut(s) 50
BstC8I GCNNGC 2 cut(s) 66, 290
BstF5I GGATG 2 cut(s) 96, 314
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 1 cut(s) 377
BstMBI GATC 1 cut(s) 374
BstMWI GCNNNNNNNGC 4 cut(s) 50, 82, 136, 365
BstNI CCWGG 5 cut(s) 102, 117, 126, 150, 192
BstNSI RCATGY 1 cut(s) 51
BstSCI CCNGG 6 cut(s) 59, 100, 115, 124, 148, 190
BstSFI CTRYAG 1 cut(s) 324
BstV1I GCAGC 5 cut(s) 65, 340, 343, 351, 380
BstV2I GAAGAC 1 cut(s) 165
BsuRI GGCC 3 cut(s) 105, 246, 263
BtsCI GGATG 2 cut(s) 96, 314
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 1 cut(s) 333
Cac8I GCNNGC 2 cut(s) 66, 290
CfoI GCGC 1 cut(s) 76
Cfr13I GGNCC 1 cut(s) 245
Csp6I GTAC 1 cut(s) 345
CviAII CATG 1 cut(s) 48
CviJI RGCY 9 cut(s) 14, 64, 105, 130, 161, 246, 263, 342, 371
CviKI_1 RGCY 9 cut(s) 14, 64, 105, 130, 161, 246, 263, 342, 371
CviQI GTAC 1 cut(s) 345
DpnI GATC 1 cut(s) 376
DpnII GATC 1 cut(s) 374
Eco130I CCWWGG 1 cut(s) 264
Eco147I AGGCCT 2 cut(s) 105, 263
Eco57I CTGAAG 1 cut(s) 164
EcoO109I RGGNCCY 1 cut(s) 245
EcoRII CCWGG 5 cut(s) 100, 115, 124, 148, 190
EcoT14I CCWWGG 1 cut(s) 264
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 1 cut(s) 51
FaiI YATR 1 cut(s) 49
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FatI CATG 1 cut(s) 47
Fnu4HI GCNGC 5 cut(s) 54, 340, 354, 357, 369
FokI GGATG 2 cut(s) 83, 301
Fsp4HI GCNGC 5 cut(s) 54, 340, 354, 357, 369
FspBI CTAG 4 cut(s) 6, 141, 242, 372
GlaI GCGC 1 cut(s) 75
GluI GCNGC 5 cut(s) 54, 340, 354, 357, 369
HaeIII GGCC 3 cut(s) 105, 246, 263
HapII CCGG 1 cut(s) 60
HhaI GCGC 1 cut(s) 76
Hin1II CATG 1 cut(s) 51
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HinfI GANTC 1 cut(s) 121
HpaII CCGG 1 cut(s) 60
HphI GGTGA 1 cut(s) 164
Hpy188I TCNGA 2 cut(s) 183, 205
Hpy188III TCNNGA 4 cut(s) 6, 91, 223, 334
HpyAV CCTTC 1 cut(s) 97
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 4 cut(s) 53, 200, 258, 326
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 82, 136, 365
HpySE526I ACGT 1 cut(s) 186
Hsp92II CATG 1 cut(s) 51
HspAI GCGC 1 cut(s) 74
Kzo9I GATC 1 cut(s) 374
LmnI GCTCC 1 cut(s) 364
Lsp1109I GCAGC 5 cut(s) 65, 340, 343, 351, 380
LweI GCATC 2 cut(s) 61, 209
MaeI CTAG 4 cut(s) 6, 141, 242, 372
MaeII ACGT 1 cut(s) 186
MalI GATC 1 cut(s) 376
MboI GATC 1 cut(s) 374
MboII GAAGA 2 cut(s) 126, 165
MhlI GDGCHC 1 cut(s) 333
MluCI AATT 3 cut(s) 207, 233, 283
MnlI CCTC 1 cut(s) 123
MroXI GAANNNNTTC 1 cut(s) 299
MspA1I CMGCKG 1 cut(s) 342
MspI CCGG 1 cut(s) 60
MspR9I CCNGG 6 cut(s) 61, 102, 117, 126, 150, 192
MvaI CCWGG 5 cut(s) 102, 117, 126, 150, 192
MwoI GCNNNNNNNGC 4 cut(s) 50, 82, 136, 365
NciI CCSGG 1 cut(s) 61
NdeII GATC 1 cut(s) 374
NlaIII CATG 1 cut(s) 51
NspI RCATGY 1 cut(s) 51
PceI AGGCCT 2 cut(s) 105, 263
PciI ACATGT 1 cut(s) 47
PdmI GAANNNNTTC 1 cut(s) 299
PfeI GAWTC 1 cut(s) 121
PflMI CCANNNNNTGG 1 cut(s) 168
PkrI GCNGC 5 cut(s) 55, 341, 355, 358, 370
PscI ACATGT 1 cut(s) 47
Psp1406I AACGTT 1 cut(s) 186
Psp6I CCWGG 5 cut(s) 100, 115, 124, 148, 190
PspGI CCWGG 5 cut(s) 100, 115, 124, 148, 190
PspPI GGNCC 1 cut(s) 245
PstI CTGCAG 1 cut(s) 328
PvuII CAGCTG 1 cut(s) 342
RsaI GTAC 1 cut(s) 346
RsaNI GTAC 1 cut(s) 345
SatI GCNGC 5 cut(s) 54, 340, 354, 357, 369
Sau3AI GATC 1 cut(s) 374
Sau96I GGNCC 1 cut(s) 245
ScrFI CCNGG 6 cut(s) 61, 102, 117, 126, 150, 192
SduI GDGCHC 1 cut(s) 333
SetI ASST 5 cut(s) 16, 154, 189, 344, 373
SfaNI GCATC 2 cut(s) 61, 209
SfcI CTRYAG 1 cut(s) 324
SmlI CTYRAG 2 cut(s) 15, 332
SmoI CTYRAG 2 cut(s) 15, 332
Sse9I AATT 3 cut(s) 207, 233, 283
SseBI AGGCCT 2 cut(s) 105, 263
SsiI CCGC 2 cut(s) 83, 292
SspMI CTAG 4 cut(s) 6, 141, 242, 372
StuI AGGCCT 2 cut(s) 105, 263
StyD4I CCNGG 6 cut(s) 59, 100, 115, 124, 148, 190
StyI CCWWGG 1 cut(s) 264
TaiI ACGT 1 cut(s) 189
TaqI TCGA 2 cut(s) 92, 270
TasI AATT 3 cut(s) 207, 233, 283
TatI WGTACW 1 cut(s) 344
TfiI GAWTC 1 cut(s) 121
TscAI CASTG 1 cut(s) 333
TseI GCWGC 5 cut(s) 53, 339, 353, 356, 368
TspRI CASTG 1 cut(s) 333
Van91I CCANNNNNTGG 1 cut(s) 168
XapI RAATTY 2 cut(s) 207, 233
XbaI TCTAGA 1 cut(s) 5
XceI RCATGY 1 cut(s) 51
XmnI GAANNNNTTC 1 cut(s) 299
XspI CTAG 4 cut(s) 6, 141, 242, 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.