AT2G29200

Pumilio homolog 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002776
Physical Location & Seq
Reverse (-)
12548931 .. 12553711
4781 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G29200.1

Sequence Viewer

Length: 2907 bp
ATGATACCGGAACTAGGGAGAAGACCGATGCATAGAGGTAACGAAGATTCATCTTTTGGGGATGATTATGAGAAAGAGATTGGAGTATTGCTTGGTGAGCAGCAGAGAAGGCAAGTAGAAGCTGATGAGCTTGAGAGAGAGCTTAACTTGTTTAGAAGTGGCTCTGCTCCACCGACTGTTGATGGTTCTGTTAGTGCTGCCGGAGGGCTTTTTAGCGGCGGAGGTGGGGCTCCTTTTTTGGAGTTTGGTGGTGTTAATAAGGGTAATGGGTTTGGTGGTGATGATGAGGAGTTTAGGAAAGATCCGGCTTACTTGTCTTATTACTATGCTAATATGAAGTTGAATCCGAGGTTGCCACCGCCTTTGATGTCTAGGGAGGATTTGAGGGTTGCTCAGAGGCTTAAAGGGAGTAATAATGTGTTAGGTGGTGTTGGGGATAGGAGAAAAGTTAATGATAATCGATCTTTGTTCTCTATGCCACCGGGTTTTGAAGGTGAGAAAACTGGTGCTTCTGCTTCTGAATGGGATGCTAATGGATTGATTGGTTTACCGGGTTTGGGACTTGGAGGCAAGCAGAAGAGTTTTGCTGATATCTTTCAGGCTGATATGGGGCATGGACATCCTGTCGCACAACAGCCCTCACGTCCTGCAAGTCGTAATACCTTTGATGAAAATGTTGACTCTAATAATAATCTGTCTCCGTCTGCATCACAAGGCATTGGTGCACCATCTCCATACAGCTATGCAGCTGTTCTAGGCTCTTCTTTGTCTAGAAATGGAACTCCAGATCCACAGGCCATTGCTAGGGTGCCTAGTCCCTGTCTTACTCCCATTGGGAGTGGAAGAATGAGTTCAAATGATAAGAGGAACACAAGTAACCAGAGCCCATTCAATGGTGTTACCTCTGGTCTCAACGAGTCTTCTGATCTTGTCAATGCTTTATCTGGCTTGAACCTGTCATGCAGTGTTGGGTTAGATGATCGGAGTCAGGCTGAGCAAGATGTCGAAAAGGTCAGGAACTATATGTTTGGACTGCAAGGTGGGCATAACGAAGTCAATCAACATGAGTTCCCAAACAAATCTGATCAAGCCCATAAGGCAACTGGCTCGTTGAGGAATTCACAATTGAGAGGGCCACACGGATCTGCCTATAATGGTGGAGTTGGATTAGCTAATCCTTACCAGCAACTTGATAGCCCAAATTATTGTCTCAACAATTACGCATTAAATCCAGCTGTAGCCTCTATGATGGCTAACCAACTTGGGAACAATAACTTTGCTCCAATGTATGATAATGTATCCGCTTTGGGATTTTCTGGAATGGACTCTAGACATCATGGACGAGGCTTTGTATCTTCTGGTCAAAACCTCTCTGAATCACGTAATCTTGGCAGATTCAGTAATCGAATGATGGGAGGAGGTGCTGGTCTCCAGTCACATATGGTAGACCCAATGTATAATCAGTATGCTGATTCACTTGATCTTCTCAATGATCCTTCCATGGATAGGAACTTTATGGGTGGTTCATCATACATGGATATGCTTGAACTCCAGAGGGCTTATCTTGGAGCTCAGAAATCACAGTATGGTGTCCCTTACAAATCCGGGAGCCCTAACAGCCATAGCTATTATGGGAGTCCAACATTTGGGTCTAATATGTCATATCCTGGCAGCCCATTGGCTCATCATGGTATGCCAAACTCTCTTATGTCACCTTATAGCCCTATGAGACGGGATGAAGTTAATATGCGATTTCCTTCTGCAACAAGAAATTATTCGGGAGGGTTAATGGGTTCTTGGCACATGGATGCTAGTTTTGATGAAGGCTTTGGATCTTCAATGCTTGAAGAGTTCAAAAGTAACAAAACAAGAGGATTTGAACTTTCTGAAATAGCTGGCCATGTTGTGGAGTTCAGTTCGGATCAATATGGAAGCCGCTTTATTCAGCAGAAACTCGAGACAGCAACAACTGATGAGAAAAACATGGTCTATGAGGAGATCATGCCACAAGCTCTTGTCCTGATGACTGACGTTTTTGGAAACTATGTGATTCAGAAGTTCTTTGAGCATGGACTCCCGCCACAGAGGAGAGAATTGGCAGAGAAACTCTTTGACCATGTTTTGCCTCTTAGCTTACAGATGTATGGTTGCCGTGTCATCCAGAAGGCAATTGAGGTGGTTGATCTAGACCAGAAAATTAAAATGGTGAAAGAACTTGATGGACATGTAATGCGATGTGTACGTGATCAGAATGGGAACCATGTGGTTCAAAAGTGTATTGAATGTGTGCCTGAAGAAAACATCGAGTTTATTATTTCAACTTTCTTTGGCCATGTGGTGACCCTCTCCACTCACCCATATGGGTGCCGTGTTATTCAGAGAGTATTGGAACACTGCCATGACCCTGATACACAGAGTAAGGTTATGGAAGAAATCCTGTCCACTGTTAGTATGCTGGCCCAAGATCAGTATGGAAACTATGTTGTTCAGCATGTGCTGGAGCATGGAAAGCCTGATGAGCGCACTGTGATAATCAAAGAATTGGCGGGGAAGATTGTTCAGATGAGCCAGCAGAAGTTCGCATCAAACGTTGTTGAGAAATGTTTGACTTTTGGAGGTCCTGAGGAACGAGAATTGCTTGTGAATGAGATGCTGGGAACAACTGATGAAAACGAGCCTCTTCAGGCGATGATGAAGGATCAGTTCGCAAACTATGTAGTGCAGAAAGTTTTGGAGACCTGTGATGACCAACAACGTGAGCTGATACTTACTCGCATCAAAGTGCATCTTACTGCTTTAAAAAAGTACACTTATGGAAAGCATGTCGTTGCGCGTATTGAAAAACTCGTTGCTGCTGGAGAGAGGAGAATGGCTTTGCAGTCCCTAACCCAGCCCCAGATGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0071944 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

968

Amino Acids

106.56

Weight (kDa)

5.74

Isoelectric Point (pI)

50.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 269 - 365 6.1e-30 Nucleic acid binding protein NABP
NABP PF07990 365 - 629 4.5e-92 Nucleic acid binding protein NABP
PUF PF00806 634 - 668 4.1e-11 Pumilio-family RNA binding repeat
PUF PF00806 673 - 703 1.9e-07 Pumilio-family RNA binding repeat
PUF PF00806 706 - 739 6.9e-09 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 735 - 823 4.5e-08 NOP9-like PUF repeat domain
PUF PF00806 748 - 772 3.4e-06 Pumilio-family RNA binding repeat
PUF PF00806 778 - 813 6.1e-13 Pumilio-family RNA binding repeat
PUF PF00806 818 - 848 3.8e-10 Pumilio-family RNA binding repeat
PUF PF00806 852 - 885 4e-08 Pumilio-family RNA binding repeat
PUF PF00806 897 - 926 2.8e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 808, 2364
AccB7I CCANNNNNTGG 3 cut(s) 893, 1646, 1906
AccI GTMKAC 1 cut(s) 1446
AccII CGCG 1 cut(s) 2833
AciI CCGC 7 cut(s) 216, 219, 359, 1302, 1936, 2078, 2546
AclI AACGTT 1 cut(s) 2589
AclWI GGATC 7 cut(s) 296, 782, 1150, 1487, 1840, 1929, 2706
AcoI YGGCCR 2 cut(s) 1897, 2329
AcsI RAATTY 1 cut(s) 1117
AcuI CTGAAG 2 cut(s) 2313, 2666
AfaI GTAC 2 cut(s) 2241, 2807
AfiI CCNNNNNNNGG 7 cut(s) 14, 557, 804, 893, 1506, 1646, 1906
AflIII ACRYGT 1 cut(s) 2224
AhdI GACNNNNNGTC 1 cut(s) 623
AjiI CACGTC 1 cut(s) 644
AjnI CCWGG 1 cut(s) 1666
AloI GAACNNNNNNTCC 2 cut(s) 772, 804
Alw21I GWGCWC 2 cut(s) 727, 1573
Alw26I GTCTC 7 cut(s) 702, 914, 1214, 1433, 1723, 1952, 2729
Alw44I GTGCAC 1 cut(s) 723
AlwI GGATC 7 cut(s) 296, 782, 1150, 1487, 1840, 1929, 2706
Ama87I CYCGRG 1 cut(s) 1955
AoxI GGCC 5 cut(s) 795, 1133, 1897, 2329, 2457
ApaLI GTGCAC 1 cut(s) 723
ApeKI GCWGC 5 cut(s) 100, 197, 746, 1671, 2852
ApoI RAATTY 1 cut(s) 1117
Asp700I GAANNNNTTC 2 cut(s) 850, 1774
AspLEI GCGC 2 cut(s) 2523, 2833
AspS9I GGNCC 3 cut(s) 1133, 2458, 2618
AsuC2I CCSGG 3 cut(s) 483, 552, 1606
AsuHPI GGTGA 7 cut(s) 107, 290, 506, 1704, 2218, 2345, 2350
AvaI CYCGRG 1 cut(s) 1955
AvaII GGWCC 1 cut(s) 2618
AxyI CCTNAGG 1 cut(s) 2622
BaeGI GKGCMC 1 cut(s) 727
BalI TGGCCA 2 cut(s) 1899, 2331
BanI GGYRCC 2 cut(s) 808, 2364
BanII GRGCYC 4 cut(s) 232, 887, 1573, 1613
BbsI GAAGAC 2 cut(s) 28, 912
Bbv12I GWGCWC 2 cut(s) 727, 1573
BbvI GCAGC 5 cut(s) 112, 184, 758, 1683, 2839
BccI CCATC 6 cut(s) 176, 736, 1243, 1405, 2213, 2893
BceAI ACGGC 2 cut(s) 2136, 2352
BcgI CGANNNNNNTGC 2 cut(s) 1089, 1123
BciT130I CCWGG 1 cut(s) 1668
BciVI GTATCC 1 cut(s) 1309
BclI TGATCA 2 cut(s) 1084, 2245
BcnI CCSGG 3 cut(s) 483, 552, 1606
BcoDI GTCTC 7 cut(s) 702, 914, 1214, 1433, 1723, 1952, 2729
BfaI CTAG 9 cut(s) 14, 372, 755, 771, 804, 813, 1329, 1812, 2186
BfmI CTRYAG 1 cut(s) 1236
BfuI GTATCC 1 cut(s) 1309
BglI GCCNNNNNGGC 1 cut(s) 1097
BisI GCNGC 7 cut(s) 101, 198, 217, 747, 1672, 1936, 2853
BlpI GCTNAGC 1 cut(s) 993
BlsI GCNGC 7 cut(s) 102, 199, 218, 748, 1673, 1937, 2854
Bme1390I CCNGG 4 cut(s) 483, 552, 1606, 1668
Bme18I GGWCC 1 cut(s) 2618
BmeRI GACNNNNNGTC 1 cut(s) 623
BmeT110I CYCGRG 1 cut(s) 1955
BmgBI CACGTC 1 cut(s) 644
BmgT120I GGNCC 3 cut(s) 1133, 2458, 2618
BmiI GGNNCC 5 cut(s) 231, 810, 1610, 2258, 2366
BmrFI CCNGG 4 cut(s) 483, 552, 1606, 1668
BmsI GCATC 8 cut(s) 18, 517, 716, 1798, 2591, 2640, 2784, 2794
BpiI GAAGAC 2 cut(s) 28, 912
BplI GAGNNNNNCTC 2 cut(s) 376, 408
BpmI CTGGAG 5 cut(s) 768, 1415, 1535, 2519, 2877
Bpu1102I GCTNAGC 1 cut(s) 993
BpuEI CTTGAG 1 cut(s) 152
BpuMI CCSGG 3 cut(s) 483, 552, 1606
Bsa29I ATCGAT 1 cut(s) 460
BsaAI YACGTR 2 cut(s) 1382, 2243
BsaI GGTCTC 3 cut(s) 914, 1433, 2729
BsaJI CCNNGG 2 cut(s) 347, 1500
BsaWI WCCGGW 1 cut(s) 7
Bsc4I CCNNNNNNNGG 7 cut(s) 14, 557, 804, 893, 1506, 1646, 1906
Bse1I ACTGG 3 cut(s) 508, 1108, 1432
Bse21I CCTNAGG 1 cut(s) 2622
Bse3DI GCAATG 1 cut(s) 798
BseBI CCWGG 1 cut(s) 1668
BseCI ATCGAT 1 cut(s) 460
BseDI CCNNGG 2 cut(s) 347, 1500
BseGI GGATG 6 cut(s) 67, 532, 619, 1741, 1813, 2157
BseLI CCNNNNNNNGG 7 cut(s) 14, 557, 804, 893, 1506, 1646, 1906
BseMI GCAATG 1 cut(s) 798
BseMII CTCAG 4 cut(s) 407, 984, 1586, 2613
BseNI ACTGG 3 cut(s) 508, 1108, 1432
BseRI GAGGAG 5 cut(s) 302, 1431, 2009, 2101, 2878
BseSI GKGCMC 1 cut(s) 727
BseXI GCAGC 5 cut(s) 112, 184, 758, 1683, 2839
BseYI CCCAGC 2 cut(s) 2653, 2889
BsgI GTGCAG 1 cut(s) 2741
Bsh1236I CGCG 1 cut(s) 2833
BshFI GGCC 5 cut(s) 797, 1135, 1899, 2331, 2459
BshNI GGYRCC 2 cut(s) 808, 2364
BshVI ATCGAT 1 cut(s) 460
BsiHKAI GWGCWC 2 cut(s) 727, 1573
BsiHKCI CYCGRG 1 cut(s) 1955
BsiSI CCGG 6 cut(s) 8, 201, 305, 482, 551, 1605
BslFI GGGAC 4 cut(s) 573, 801, 1577, 2866
BslI CCNNNNNNNGG 7 cut(s) 14, 557, 804, 893, 1506, 1646, 1906
BsmAI GTCTC 7 cut(s) 702, 914, 1214, 1433, 1723, 1952, 2729
BsmBI CGTCTC 1 cut(s) 1723
BsmFI GGGAC 4 cut(s) 573, 801, 1577, 2866
BsnI GGCC 5 cut(s) 797, 1135, 1899, 2331, 2459
Bso31I GGTCTC 3 cut(s) 914, 1433, 2729
BsoBI CYCGRG 1 cut(s) 1955
Bsp1286I GDGCHC 5 cut(s) 232, 727, 887, 1573, 1613
Bsp1720I GCTNAGC 1 cut(s) 993
Bsp19I CCATGG 1 cut(s) 1500
BspACI CCGC 7 cut(s) 216, 219, 359, 1302, 1936, 2078, 2546
BspANI GGCC 5 cut(s) 797, 1135, 1899, 2331, 2459
BspCNI CTCAG 4 cut(s) 406, 985, 1585, 2614
BspDI ATCGAT 1 cut(s) 460
BspFNI CGCG 1 cut(s) 2833
BspLI GGNNCC 5 cut(s) 231, 810, 1610, 2258, 2366
BspPI GGATC 7 cut(s) 296, 782, 1150, 1487, 1840, 1929, 2706
BspQI GCTCTTC 1 cut(s) 766
BspT107I GGYRCC 2 cut(s) 808, 2364
BspTNI GGTCTC 3 cut(s) 914, 1433, 2729
BsrDI GCAATG 1 cut(s) 798
BsrI ACTGG 3 cut(s) 508, 1108, 1432
BssECI CCNNGG 2 cut(s) 347, 1500
BssT1I CCWWGG 1 cut(s) 1500
Bst2UI CCWGG 1 cut(s) 1668
Bst4CI ACNGT 4 cut(s) 178, 1584, 2446, 2527
Bst6I CTCTTC 4 cut(s) 572, 766, 1842, 2685
BstBAI YACGTR 2 cut(s) 1382, 2243
BstC8I GCNNGC 4 cut(s) 572, 1897, 2457, 2570
BstDEI CTNAG 5 cut(s) 393, 993, 1572, 2129, 2622
BstDSI CCRYGG 1 cut(s) 1500
BstEII GGTNACC 1 cut(s) 2338
BstF5I GGATG 6 cut(s) 67, 532, 619, 1741, 1813, 2157
BstFNI CGCG 1 cut(s) 2833
BstHHI GCGC 2 cut(s) 2523, 2833
BstMAI GTCTC 7 cut(s) 702, 914, 1214, 1433, 1723, 1952, 2729
BstMWI GCNNNNNNNGC 7 cut(s) 97, 109, 1042, 1097, 1617, 2509, 2518
BstNI CCWGG 1 cut(s) 1668
BstNSI RCATGY 3 cut(s) 2228, 2495, 2825
BstPI GGTNACC 1 cut(s) 2338
BstSCI CCNGG 4 cut(s) 481, 550, 1604, 1666
BstSFI CTRYAG 1 cut(s) 1236
BstSLI GKGCMC 1 cut(s) 727
BstUI CGCG 1 cut(s) 2833
BstV1I GCAGC 5 cut(s) 112, 184, 758, 1683, 2839
BstV2I GAAGAC 2 cut(s) 28, 912
BstX2I RGATCY 4 cut(s) 301, 787, 1142, 1832
BstYI RGATCY 4 cut(s) 301, 787, 1142, 1832
Bsu15I ATCGAT 1 cut(s) 460
Bsu36I CCTNAGG 1 cut(s) 2622
BsuI GTATCC 1 cut(s) 1309
BsuRI GGCC 5 cut(s) 797, 1135, 1899, 2331, 2459
BsuTUI ATCGAT 1 cut(s) 460
BtgI CCRYGG 1 cut(s) 1500
BtgZI GCGATG 2 cut(s) 2248, 2702
BtrI CACGTC 1 cut(s) 644
BtsCI GGATG 6 cut(s) 67, 532, 619, 1741, 1813, 2157
BtsI GCAGTG 2 cut(s) 970, 2392
BtsIMutI CAGTG 4 cut(s) 970, 2392, 2442, 2523
Cac8I GCNNGC 4 cut(s) 572, 1897, 2457, 2570
CfoI GCGC 2 cut(s) 2523, 2833
Cfr13I GGNCC 3 cut(s) 1133, 2458, 2618
ClaI ATCGAT 1 cut(s) 460
Csp6I GTAC 2 cut(s) 2240, 2806
CspCI CAANNNNNGTGG 4 cut(s) 345, 380, 2157, 2192
CviQI GTAC 2 cut(s) 2240, 2806
DdeI CTNAG 5 cut(s) 393, 993, 1572, 2129, 2622
DraI TTTAAA 1 cut(s) 2799
DriI GACNNNNNGTC 1 cut(s) 623
EaeI YGGCCR 2 cut(s) 1897, 2329
Eam1104I CTCTTC 4 cut(s) 572, 766, 1842, 2685
Eam1105I GACNNNNNGTC 1 cut(s) 623
EarI CTCTTC 4 cut(s) 572, 766, 1842, 2685
EciI GGCGGA 1 cut(s) 234
Ecl136II GAGCTC 1 cut(s) 1571
Eco130I CCWWGG 1 cut(s) 1500
Eco24I GRGCYC 4 cut(s) 232, 887, 1573, 1613
Eco31I GGTCTC 3 cut(s) 914, 1433, 2729
Eco32I GATATC 1 cut(s) 592
Eco47I GGWCC 1 cut(s) 2618
Eco53kI GAGCTC 1 cut(s) 1571
Eco57I CTGAAG 2 cut(s) 2313, 2666
Eco81I CCTNAGG 1 cut(s) 2622
Eco88I CYCGRG 1 cut(s) 1955
Eco91I GGTNACC 1 cut(s) 2338
EcoICRI GAGCTC 1 cut(s) 1571
EcoO109I RGGNCCY 1 cut(s) 2618
EcoO65I GGTNACC 1 cut(s) 2338
EcoRI GAATTC 1 cut(s) 1117
EcoRII CCWGG 1 cut(s) 1666
EcoRV GATATC 1 cut(s) 592
EcoT14I CCWWGG 1 cut(s) 1500
EcoT22I ATGCAT 1 cut(s) 33
EcoT38I GRGCYC 4 cut(s) 232, 887, 1573, 1613
ErhI CCWWGG 1 cut(s) 1500
Esp3I CGTCTC 1 cut(s) 1723
FalI AAGNNNNNCTT 2 cut(s) 2772, 2804
FaqI GGGAC 4 cut(s) 573, 801, 1577, 2866
FauI CCCGC 2 cut(s) 2085, 2539
FauNDI CATATG 2 cut(s) 1440, 2359
FbaI TGATCA 2 cut(s) 1084, 2245
FblI GTMKAC 1 cut(s) 1446
Fnu4HI GCNGC 7 cut(s) 101, 198, 217, 747, 1672, 1936, 2853
FokI GGATG 6 cut(s) 74, 539, 606, 1748, 1820, 2144
FriOI GRGCYC 4 cut(s) 232, 887, 1573, 1613
Fsp4HI GCNGC 7 cut(s) 101, 198, 217, 747, 1672, 1936, 2853
FspBI CTAG 9 cut(s) 14, 372, 755, 771, 804, 813, 1329, 1812, 2186
GlaI GCGC 2 cut(s) 2522, 2832
GluI GCNGC 7 cut(s) 101, 198, 217, 747, 1672, 1936, 2853
GsaI CCCAGC 2 cut(s) 2657, 2893
GsuI CTGGAG 5 cut(s) 768, 1415, 1535, 2519, 2877
HaeIII GGCC 5 cut(s) 797, 1135, 1899, 2331, 2459
HapII CCGG 6 cut(s) 8, 201, 305, 482, 551, 1605
HhaI GCGC 2 cut(s) 2523, 2833
Hin6I GCGC 2 cut(s) 2521, 2831
HinP1I GCGC 2 cut(s) 2521, 2831
HincII GTYRAC 1 cut(s) 679
HindII GTYRAC 1 cut(s) 679
HpaII CCGG 6 cut(s) 8, 201, 305, 482, 551, 1605
HphI GGTGA 7 cut(s) 107, 290, 506, 1704, 2218, 2345, 2350
Hpy166II GTNNAC 7 cut(s) 548, 679, 725, 1447, 2240, 2442, 2808
Hpy8I GTNNAC 7 cut(s) 548, 679, 725, 1447, 2240, 2442, 2808
HpyAV CCTTC 7 cut(s) 102, 485, 1506, 1767, 1817, 2158, 2689
HpyCH4III ACNGT 4 cut(s) 178, 1584, 2446, 2527
HpyCH4IV ACGT 6 cut(s) 643, 1381, 2031, 2242, 2589, 2755
HpyF10VI GCNNNNNNNGC 7 cut(s) 97, 109, 1042, 1097, 1617, 2509, 2518
HpyF3I CTNAG 5 cut(s) 393, 993, 1572, 2129, 2622
HpySE526I ACGT 6 cut(s) 643, 1381, 2031, 2242, 2589, 2755
HspAI GCGC 2 cut(s) 2521, 2831
Ksp22I TGATCA 2 cut(s) 1084, 2245
LguI GCTCTTC 1 cut(s) 766
LmnI GCTCC 6 cut(s) 172, 235, 1285, 1568, 1608, 2500
Lsp1109I GCAGC 5 cut(s) 112, 184, 758, 1683, 2839
LweI GCATC 8 cut(s) 18, 517, 716, 1798, 2591, 2640, 2784, 2794
MaeI CTAG 9 cut(s) 14, 372, 755, 771, 804, 813, 1329, 1812, 2186
MaeII ACGT 6 cut(s) 643, 1381, 2031, 2242, 2589, 2755
MaeIII GTNAC 7 cut(s) 38, 875, 898, 1434, 1710, 1859, 2338
MfeI CAATTG 2 cut(s) 1124, 2169
MflI RGATCY 4 cut(s) 301, 787, 1142, 1832
MhlI GDGCHC 5 cut(s) 232, 727, 887, 1573, 1613
MlsI TGGCCA 2 cut(s) 1899, 2331
MluNI TGGCCA 2 cut(s) 1899, 2331
MlyI GAGTC 6 cut(s) 674, 926, 994, 1319, 1645, 2067
MmeI TCCRAC 2 cut(s) 1144, 1664
Mox20I TGGCCA 2 cut(s) 1899, 2331
Mph1103I ATGCAT 1 cut(s) 33
MroXI GAANNNNTTC 2 cut(s) 850, 1774
MscI TGGCCA 2 cut(s) 1899, 2331
MslI CAYNNNNRTG 6 cut(s) 1538, 1806, 2358, 2362, 2397, 2780
Msp20I TGGCCA 2 cut(s) 1899, 2331
MspA1I CMGCKG 2 cut(s) 749, 1235
MspI CCGG 6 cut(s) 8, 201, 305, 482, 551, 1605
MspR9I CCNGG 4 cut(s) 483, 552, 1606, 1668
MunI CAATTG 2 cut(s) 1124, 2169
MvaI CCWGG 1 cut(s) 1668
MvnI CGCG 1 cut(s) 2833
MwoI GCNNNNNNNGC 7 cut(s) 97, 109, 1042, 1097, 1617, 2509, 2518
NciI CCSGG 3 cut(s) 483, 552, 1606
NcoI CCATGG 1 cut(s) 1500
NdeI CATATG 2 cut(s) 1440, 2359
NlaIV GGNNCC 5 cut(s) 231, 810, 1610, 2258, 2366
NmuCI GTSAC 3 cut(s) 1434, 1710, 2338
NsiI ATGCAT 1 cut(s) 33
NspI RCATGY 3 cut(s) 2228, 2495, 2825
PaeR7I CTCGAG 1 cut(s) 1955
PciI ACATGT 1 cut(s) 2224
PciSI GCTCTTC 1 cut(s) 766
PcsI WCGNNNNNNNCGW 1 cut(s) 2586
PdmI GAANNNNTTC 2 cut(s) 850, 1774
PfeI GAWTC 6 cut(s) 47, 343, 1376, 1395, 1472, 2050
PflMI CCANNNNNTGG 3 cut(s) 893, 1646, 1906
PfoI TCCNGGA 1 cut(s) 1604
PkrI GCNGC 7 cut(s) 102, 199, 218, 748, 1673, 1937, 2854
PleI GAGTC 6 cut(s) 674, 925, 993, 1319, 1644, 2067
PpsI GAGTC 6 cut(s) 674, 925, 993, 1319, 1644, 2067
Ppu21I YACGTR 2 cut(s) 1382, 2243
PpuMI RGGWCCY 1 cut(s) 2618
PscI ACATGT 1 cut(s) 2224
Psp124BI GAGCTC 1 cut(s) 1573
Psp1406I AACGTT 1 cut(s) 2589
Psp5II RGGWCCY 1 cut(s) 2618
Psp6I CCWGG 1 cut(s) 1666
PspEI GGTNACC 1 cut(s) 2338
PspFI CCCAGC 2 cut(s) 2653, 2889
PspGI CCWGG 1 cut(s) 1666
PspN4I GGNNCC 5 cut(s) 231, 810, 1610, 2258, 2366
PspPI GGNCC 3 cut(s) 1133, 2458, 2618
PspPPI RGGWCCY 1 cut(s) 2618
PsuI RGATCY 4 cut(s) 301, 787, 1142, 1832
PvuII CAGCTG 2 cut(s) 749, 1235
RsaI GTAC 2 cut(s) 2241, 2807
RsaNI GTAC 2 cut(s) 2240, 2806
RseI CAYNNNNRTG 6 cut(s) 1538, 1806, 2358, 2362, 2397, 2780
SacI GAGCTC 1 cut(s) 1573
SapI GCTCTTC 1 cut(s) 766
SatI GCNGC 7 cut(s) 101, 198, 217, 747, 1672, 1936, 2853
Sau96I GGNCC 3 cut(s) 1133, 2458, 2618
SchI GAGTC 6 cut(s) 674, 926, 994, 1319, 1645, 2067
ScrFI CCNGG 4 cut(s) 483, 552, 1606, 1668
SduI GDGCHC 5 cut(s) 232, 727, 887, 1573, 1613
SfaNI GCATC 8 cut(s) 18, 517, 716, 1798, 2591, 2640, 2784, 2794
SfcI CTRYAG 1 cut(s) 1236
Sfr274I CTCGAG 1 cut(s) 1955
SinI GGWCC 1 cut(s) 2618
SlaI CTCGAG 1 cut(s) 1955
SmiMI CAYNNNNRTG 6 cut(s) 1538, 1806, 2358, 2362, 2397, 2780
SmlI CTYRAG 2 cut(s) 131, 1955
SmoI CTYRAG 2 cut(s) 131, 1955
SsiI CCGC 7 cut(s) 216, 219, 359, 1302, 1936, 2078, 2546
SspMI CTAG 9 cut(s) 14, 372, 755, 771, 804, 813, 1329, 1812, 2186
SstI GAGCTC 1 cut(s) 1573
StyD4I CCNGG 4 cut(s) 481, 550, 1604, 1666
StyI CCWWGG 1 cut(s) 1500
TaaI ACNGT 4 cut(s) 178, 1584, 2446, 2527
TaiI ACGT 6 cut(s) 646, 1384, 2034, 2245, 2592, 2758
TaqI TCGA 5 cut(s) 460, 1005, 1405, 1956, 2304
TaqII GACCGA 1 cut(s) 40
TatI WGTACW 1 cut(s) 2805
TauI GCSGC 2 cut(s) 219, 1938
TfiI GAWTC 6 cut(s) 47, 343, 1376, 1395, 1472, 2050
TscAI CASTG 4 cut(s) 970, 2399, 2449, 2530
TseFI GTSAC 3 cut(s) 1434, 1710, 2338
TseI GCWGC 5 cut(s) 100, 197, 746, 1671, 2852
Tsp45I GTSAC 3 cut(s) 1434, 1710, 2338
TspDTI ATGAA 8 cut(s) 39, 350, 684, 1515, 1752, 1836, 2682, 2708
TspGWI ACGGA 2 cut(s) 690, 1155
TspRI CASTG 4 cut(s) 970, 2399, 2449, 2530
Van91I CCANNNNNTGG 3 cut(s) 893, 1646, 1906
VneI GTGCAC 1 cut(s) 723
VpaK11BI GGWCC 1 cut(s) 2618
XapI RAATTY 1 cut(s) 1117
XbaI TCTAGA 3 cut(s) 770, 1328, 2185
XceI RCATGY 3 cut(s) 2228, 2495, 2825
XcmI CCANNNNNNNNNTGG 3 cut(s) 1100, 1628, 2468
XhoI CTCGAG 1 cut(s) 1955
XmiI GTMKAC 1 cut(s) 1446
XmnI GAANNNNTTC 2 cut(s) 850, 1774
XspI CTAG 9 cut(s) 14, 372, 755, 771, 804, 813, 1329, 1812, 2186
Zsp2I ATGCAT 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.