RchiOBHm_Chr5g0066091

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002776
Physical Location & Seq
Forward (+)
72173352 .. 72181356
8005 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34188

Sequence Viewer

Length: 3273 bp
ATGTTGTCTGAATTGGGGAGAAGGCCAATGCTTGGAGGTAGCGAGGGTTCGTTTGGGGATGAATTTGAGAAGGAGATTGGTATGTTGCTTAGGGAGCAGCGTCGGCAGGAGGCTGATGATCGTGAGAGTGACCTGAATATTTACAGGAGTGGGTCGGCGCCGCCGACTGTGGAGGGTTCTTTGAATGCGGTGGGAGGGTTGTTTGCTGGCGGTGGCGGTGGAAGTGGAGGTGGAGGTGGAGGAGGAGGAGGAGGAGGAGGGGGTGTTGGCGGTGGTGGGGGTGCTGGGTCTGTTCTTTCGGAGTTTCCTGGAGCTAAGAATGGGAATGGGTTTGCGTCGGAGGAGGAGCTGAGGTCTGATCCAGCTTATCTGCAATACTATTACTCGAATGTGAATCTGAATCCGCGGCTTCCGCCTCCTTTGTTGTCGAAGGAGGATTGGAGGTATGCACAGAGGATGAAAGGAGGGAGTTCTGTTTTGGGTGGGATTGGGGACAGGAGGAAAGTGAACAGGGCTGATGATGCTAGTGGTGGGAGATCCATGTTTTCGATGCCGCCAGGGTTTAACTCGAGGAAACAAGAGAGTGAGGTGGAGCCGGACAAAGTGCGTGGCTCAGCGGAGTGGGGTGTTGATGGGCTAATTGGTTTGCCAGGTTTAGGACTTGGCAACAAGCAAAAAAGCCTTGCTGAAATCTTTCAGGATGACTTGGGGCGTACGACTCCTGTTCCTGGGCTTCCTTCTCGTCCAGCCAGCCGTAATGCATTTGATGAAAATGTAGAGGGACTTGGTTCTGCTGAAGCAGACCTGGCTCATTTGCACCGTGACTTGATGACATCTGATGCTTTAAGATCTGGTGCAAATGGCCAGGGCATGTCTGCAGCCCAAAGCATGGGCCCACCTTCTTCATACTCCTATGCTGCAGCTCTAGGTGCTTCCTTGTCAAGAAGCACCACTCCTGATCCTCAGATTGTTGCTAGGGCTCCTAGTCCTTGCCTTACACCTATTGGAGGAGGAAGAGTTAGTGCTTCAGAAAAGAGAGGCATTTCTAGTCCAAGCTCCTTTAATGCTGTCTCATCTGGCATCAATGAGTCTGGAGATCTGGTAGCTGCCTTCTCCACCATGAATTTGTCTTCAAATGGTGTAATAGATGATGAGCACCATTTGCCATCACAGATCAAACAGGATGTTATTGATCACCAGAATTATCTCTTTGGTCTGCAAGGTGCTGATAGTCATGCTAAGCAACTTGCTTACTTAAAGAAGTCTGAATCTGGGAATATACATATGCCTTCTCCACAGTCGGCAAAAGGTTCGTACTCGGATTTGGGTAAGAGCAATGGGGGTGGGTCAGATCTGAACAACTCATCCCCTGACAGGCAAGTTGAGCTGCAAAAATCTGCTGTTCCTTCTAGTAACTTGTATAAAGGATCATCTGCATCGAATCTTAATGGTGGAGGTGGATTGCATAATCAGTATCAGCAAGTGGATAATGCAAATTCATCATTTTCTAACTATGGTTTAAGTGGCTACTCTATGAATCCAGCCTTGGCATCCATGGTGGCTAGCCAACTTGGTACTGGTAATCTGCCTCCTCTTTTTGAAAATGTTACTGCAGCATCAGCTATGGTACCTCCTGGAATGGATTCTAGAGTACTTGGAGGAGGTTTGGCTTCTGGACCAAACCTAGCAGCTGCTGCATCTGATTCACATAATCTTGGTAGATTAGGGAGTCCGATTGCGGGGACCGGTCTCCAGGCGCCTTATGTTGATCCTATGTATCTTCAATACTTGAGGACGTCAGAGTATGCTGCAGCACAGCTTGCTGCCCTGAATGATCCTTCTGTGGATAGGAACTACTTAGGTAATTCATACATGAATTTACTTGAACTTCAAAAGGCGTATCTTGGAGCCTTGCTGTCACCTCAGAAGTCACAGTATGGTGTTGGTGTTCCCTTGGGGGGGAAGACTGGTGGTTCAAATCATCATGGTTATTATGGAAATCATGCCTTTGGCATGTCTTATCCAGGAAGTCCCATGGCAAGTCCTGTTATTCCCAACTCTCCAGTTGCCCCTGGTAGTCCAATGAGGCACAATGATCTTAATATGTGTTATCCTTCTGGGATGAGGAATTTGAATTTGGGTGGGAGTGTCATGGGACCATGGCATCTGGATTCTGGTTGTAACATGGATGAGACCTTTGCATCATCACTCTTAGAAGAGTTTAAGAGCAACAAAGCCAAGTCTTTTGAACTTTTGGAAATCGGAGGACATGTTGTGGAATTCAGTGCGGATCAATACGGGAGTCGGTTTATTCAACAAAAACTTGAAACAGCCACTACGGATGAGAAAAACATGGTTTACCAGGAAATCATGCCTCAAGCTCTTGCTCTAATGACTGATGTGTTTGGTAATTATGTGATTCAAAAGTTTTTTGAGCACGGACTTCCATCTCAAAGAAGGGAATTGGCCAACAAGCTTTTTGGTCATGTGCTGACACTTAGCCTTCAAATGTATGGTTGTCGGGTGATACAGAAGGCAATAGAAGTTGTTGATCTGGACCAGAAGATCAAAATGGTTGAAGAACTTGACGGCCATGTCATGCGCTGTGTGCGTGACCAGAATGGGAACCATGTCATTCAGAAGTGTATTGAATGTGTCCCTGAAGAGGCCATTCATTTTATAGTCTCAACGTTTTTTGATCAAGTTGTGACCCTTTCCACTCATCCATATGGGTGTCGTGTGATACAGAGAGTGCTTGAGCACTGCAATGACCAGAATACTCAGAGCAAAGTTATGGATGAGATTTTAGGAGCTGTTAGCATGCTGGCACAAGATCAGTATGGCAATTATGTTGTTCAGCATGTACTGGAGCATGGAAAGCCACATGAGCGTTCTGCTATAATAAAGGAATTAGCTGGGAAGATAGTCCAAATGAGTCAACAGAAGTTTGCCTCTAATGTTGTAGAGAAATGCTTAGCTTTTGGTGGTCCTGCTGAAAGGGAATTACTAGTGAATGAGATGCTTGGCACTACTGATGAAAATGAGCCTCTTCAGGCAATGATGAAAGATCAGTTTGCAAACTACGTTGTACAGAAAGTGTTGGAGACTTGTGATGACCAACAACGCGAGCTGATTCTTTCACGAATTAAAGTTCATTTGAATGCATTGAAGAAATACACTTACGGGAAGCATATCGTGGCTCGTGTAGAAAAACTTGTTGCTGCTGGGGAAAGGAGAGTTGCTGCTGCTCAGTTGGCTCCTCACCCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1090

Amino Acids

116.18

Weight (kDa)

5.81

Isoelectric Point (pI)

49.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 326 - 751 2.8e-171 Nucleic acid binding protein NABP
PUF PF00806 757 - 791 2.9e-10 Pumilio-family RNA binding repeat
PUF PF00806 795 - 826 1.3e-07 Pumilio-family RNA binding repeat
PUF PF00806 829 - 862 1.8e-09 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 865 - 946 1.6e-07 NOP9-like PUF repeat domain
PUF PF00806 871 - 895 1e-05 Pumilio-family RNA binding repeat
PUF PF00806 901 - 936 7.4e-13 Pumilio-family RNA binding repeat
PUF PF00806 941 - 971 8.5e-10 Pumilio-family RNA binding repeat
PUF PF00806 975 - 1008 4.2e-07 Pumilio-family RNA binding repeat
PUF PF00806 1020 - 1049 2e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2594
AatII GACGTC 1 cut(s) 1799
Acc65I GGTACC 1 cut(s) 1627
AccB1I GGYRCC 3 cut(s) 157, 1627, 1756
AccB7I CCANNNNNTGG 2 cut(s) 32, 889
AccII CGCG 2 cut(s) 406, 3126
AclI AACGTT 1 cut(s) 2690
AclWI GGATC 7 cut(s) 353, 531, 953, 1435, 1763, 1829, 2298
AcoI YGGCCR 3 cut(s) 862, 2466, 2590
AcsI RAATTY 7 cut(s) 62, 1123, 1495, 1876, 2128, 2134, 2279
AcuI CTGAAG 4 cut(s) 816, 1011, 2682, 3035
AcyI GRCGYC 3 cut(s) 158, 1757, 1796
AfaI GTAC 7 cut(s) 715, 1316, 1576, 1629, 1653, 2865, 3090
AflIII ACRYGT 1 cut(s) 2269
AgeI ACCGGT 1 cut(s) 1745
AhlI ACTAGT 1 cut(s) 3007
AjuI GAANNNNNNNTTGG 4 cut(s) 1529, 1561, 2120, 2152
AloI GAACNNNNNNTCC 2 cut(s) 1349, 1381
Alw21I GWGCWC 3 cut(s) 1158, 2439, 2763
Alw26I GTCTC 5 cut(s) 1075, 1754, 2186, 2689, 3098
AlwI GGATC 7 cut(s) 353, 531, 953, 1435, 1763, 1829, 2298
AlwNI CAGNNNCTG 1 cut(s) 1694
Ama87I CYCGRG 1 cut(s) 568
AoxI GGCC 6 cut(s) 23, 862, 892, 2466, 2590, 2667
ApaI GGGCCC 1 cut(s) 896
ApoI RAATTY 7 cut(s) 62, 1123, 1495, 1876, 2128, 2134, 2279
AsiGI ACCGGT 1 cut(s) 1745
Asp700I GAANNNNTTC 2 cut(s) 693, 1642
Asp718I GGTACC 1 cut(s) 1627
AspLEI GCGC 3 cut(s) 160, 1759, 2604
AspS9I GGNCC 7 cut(s) 892, 893, 1676, 1743, 2156, 2557, 2987
AsuHPI GGTGA 4 cut(s) 1187, 1911, 2536, 3254
AsuNHI GCTAGC 1 cut(s) 1562
AvaI CYCGRG 1 cut(s) 568
AvaII GGWCC 5 cut(s) 1676, 1743, 2156, 2557, 2987
BaeGI GKGCMC 1 cut(s) 896
BalI TGGCCA 2 cut(s) 864, 2468
BanI GGYRCC 3 cut(s) 157, 1627, 1756
BanII GRGCYC 2 cut(s) 896, 982
BauI CACGAG 1 cut(s) 3201
BbsI GAAGAC 2 cut(s) 1122, 1970
Bbv12I GWGCWC 3 cut(s) 1158, 2439, 2763
BbvCI CCTCAGC 1 cut(s) 350
BccI CCATC 3 cut(s) 626, 1174, 2455
BceAI ACGGC 2 cut(s) 738, 2605
BcgI CGANNNNNNTGC 2 cut(s) 1293, 1327
BclI TGATCA 2 cut(s) 1192, 2698
BcoDI GTCTC 5 cut(s) 1075, 1754, 2186, 2689, 3098
BcuI ACTAGT 1 cut(s) 3007
BfmI CTRYAG 4 cut(s) 876, 918, 1611, 1809
BfoI RGCGCY 2 cut(s) 161, 1760
BglII AGATCT 3 cut(s) 848, 1096, 1351
BlpI GCTNAGC 3 cut(s) 613, 1239, 2974
BmcAI AGTACT 1 cut(s) 1653
Bme18I GGWCC 5 cut(s) 1676, 1743, 2156, 2557, 2987
BmeT110I CYCGRG 1 cut(s) 568
BmgT120I GGNCC 7 cut(s) 892, 893, 1676, 1743, 2156, 2557, 2987
BmtI GCTAGC 1 cut(s) 1566
BpiI GAAGAC 2 cut(s) 1122, 1970
BpmI CTGGAG 5 cut(s) 330, 1113, 1736, 2046, 2888
Bpu10I CCTNAGC 2 cut(s) 89, 350
Bpu1102I GCTNAGC 3 cut(s) 613, 1239, 2974
BpuEI CTTGAG 3 cut(s) 1810, 2361, 2777
BsaHI GRCGYC 3 cut(s) 158, 1757, 1796
BsaI GGTCTC 2 cut(s) 1754, 2186
BsaWI WCCGGW 1 cut(s) 1745
Bse118I RCCGGY 1 cut(s) 1745
Bse1I ACTGG 4 cut(s) 1582, 1972, 2063, 2871
Bse3DI GCAATG 3 cut(s) 1342, 2773, 3063
BseMI GCAATG 3 cut(s) 1342, 2773, 3063
BseMII CTCAG 6 cut(s) 341, 627, 977, 1937, 2795, 3263
BseNI ACTGG 4 cut(s) 1582, 1972, 2063, 2871
BseSI GKGCMC 1 cut(s) 896
BseYI CCCAGC 3 cut(s) 284, 2915, 3224
Bsh1236I CGCG 2 cut(s) 406, 3126
BshFI GGCC 6 cut(s) 25, 864, 894, 2468, 2592, 2669
BshNI GGYRCC 3 cut(s) 157, 1627, 1756
BshTI ACCGGT 1 cut(s) 1745
BsiHKAI GWGCWC 3 cut(s) 1158, 2439, 2763
BsiHKCI CYCGRG 1 cut(s) 568
BsiSI CCGG 2 cut(s) 596, 1746
BsiWI CGTACG 1 cut(s) 713
BslFI GGGAC 6 cut(s) 506, 795, 1756, 2016, 2169, 2642
BsmAI GTCTC 5 cut(s) 1075, 1754, 2186, 2689, 3098
BsmFI GGGAC 6 cut(s) 506, 795, 1756, 2016, 2169, 2642
BsmI GAATGC 2 cut(s) 190, 3166
BsnI GGCC 6 cut(s) 25, 864, 894, 2468, 2592, 2669
Bso31I GGTCTC 2 cut(s) 1754, 2186
BsoBI CYCGRG 1 cut(s) 568
Bsp120I GGGCCC 1 cut(s) 892
Bsp1286I GDGCHC 5 cut(s) 896, 982, 1158, 2439, 2763
Bsp1407I TGTACA 1 cut(s) 3088
Bsp1720I GCTNAGC 3 cut(s) 613, 1239, 2974
Bsp19I CCATGG 3 cut(s) 1554, 2034, 2159
BspANI GGCC 6 cut(s) 25, 864, 894, 2468, 2592, 2669
BspCNI CTCAG 6 cut(s) 342, 626, 976, 1936, 2794, 3262
BspFNI CGCG 2 cut(s) 406, 3126
BspMAI CTGCAG 4 cut(s) 880, 922, 1615, 1813
BspOI GCTAGC 1 cut(s) 1566
BspPI GGATC 7 cut(s) 353, 531, 953, 1435, 1763, 1829, 2298
BspT107I GGYRCC 3 cut(s) 157, 1627, 1756
BspTNI GGTCTC 2 cut(s) 1754, 2186
BsrDI GCAATG 3 cut(s) 1342, 2773, 3063
BsrFI RCCGGY 1 cut(s) 1745
BsrGI TGTACA 1 cut(s) 3088
BsrI ACTGG 4 cut(s) 1582, 1972, 2063, 2871
BssAI RCCGGY 1 cut(s) 1745
BssNI GRCGYC 3 cut(s) 158, 1757, 1796
BssSI CACGAG 1 cut(s) 3201
BssT1I CCWWGG 5 cut(s) 1545, 1554, 1953, 2034, 2159
Bst2BI CACGAG 1 cut(s) 3201
Bst4CI ACNGT 4 cut(s) 169, 821, 1299, 1935
Bst6I CTCTTC 4 cut(s) 1009, 2211, 2658, 3054
BstACI GRCGYC 3 cut(s) 158, 1757, 1796
BstAPI GCANNNNNTGC 3 cut(s) 1162, 1694, 1820
BstAUI TGTACA 1 cut(s) 3088
BstC8I GCNNGC 7 cut(s) 208, 751, 1564, 1821, 2822, 2826, 3128
BstDSI CCRYGG 4 cut(s) 404, 1554, 2034, 2159
BstENI CCTNNNNNAGG 1 cut(s) 1005
BstFNI CGCG 2 cut(s) 406, 3126
BstH2I RGCGCY 2 cut(s) 161, 1760
BstHHI GCGC 3 cut(s) 160, 1759, 2604
BstMAI GTCTC 5 cut(s) 1075, 1754, 2186, 2689, 3098
BstNSI RCATGY 5 cut(s) 874, 2017, 2273, 2824, 2864
BstSFI CTRYAG 4 cut(s) 876, 918, 1611, 1809
BstSLI GKGCMC 1 cut(s) 896
BstUI CGCG 2 cut(s) 406, 3126
BstV2I GAAGAC 2 cut(s) 1122, 1970
BstX2I RGATCY 4 cut(s) 536, 848, 1096, 1351
BstYI RGATCY 4 cut(s) 536, 848, 1096, 1351
BsuRI GGCC 6 cut(s) 25, 864, 894, 2468, 2592, 2669
BtgI CCRYGG 4 cut(s) 404, 1554, 2034, 2159
BtsI GCAGTG 1 cut(s) 2761
BtsIMutI CAGTG 2 cut(s) 2290, 2761
Cac8I GCNNGC 7 cut(s) 208, 751, 1564, 1821, 2822, 2826, 3128
CaiI CAGNNNCTG 1 cut(s) 1694
CfoI GCGC 3 cut(s) 160, 1759, 2604
Cfr10I RCCGGY 1 cut(s) 1745
Cfr13I GGNCC 7 cut(s) 892, 893, 1676, 1743, 2156, 2557, 2987
Cfr42I CCGCGG 1 cut(s) 407
CseI GACGC 2 cut(s) 89, 324
Csp6I GTAC 7 cut(s) 714, 1315, 1575, 1628, 1652, 2864, 3089
CspAI ACCGGT 1 cut(s) 1745
CspCI CAANNNNNGTGG 4 cut(s) 589, 624, 1324, 1359
CviQI GTAC 7 cut(s) 714, 1315, 1575, 1628, 1652, 2864, 3089
DinI GGCGCC 2 cut(s) 159, 1758
DrdI GACNNNNNNGTC 1 cut(s) 2594
DseDI GACNNNNNNGTC 1 cut(s) 2594
EaeI YGGCCR 3 cut(s) 862, 2466, 2590
Eam1104I CTCTTC 4 cut(s) 1009, 2211, 2658, 3054
EarI CTCTTC 4 cut(s) 1009, 2211, 2658, 3054
EciI GGCGGA 1 cut(s) 402
Eco130I CCWWGG 5 cut(s) 1545, 1554, 1953, 2034, 2159
Eco24I GRGCYC 2 cut(s) 896, 982
Eco31I GGTCTC 2 cut(s) 1754, 2186
Eco47I GGWCC 5 cut(s) 1676, 1743, 2156, 2557, 2987
Eco57I CTGAAG 4 cut(s) 816, 1011, 2682, 3035
Eco88I CYCGRG 1 cut(s) 568
EcoNI CCTNNNNNAGG 1 cut(s) 1005
EcoRI GAATTC 1 cut(s) 2279
EcoT14I CCWWGG 5 cut(s) 1545, 1554, 1953, 2034, 2159
EcoT22I ATGCAT 2 cut(s) 763, 3166
EcoT38I GRGCYC 2 cut(s) 896, 982
EgeI GGCGCC 2 cut(s) 159, 1758
EheI GGCGCC 2 cut(s) 159, 1758
ErhI CCWWGG 5 cut(s) 1545, 1554, 1953, 2034, 2159
FaqI GGGAC 6 cut(s) 506, 795, 1756, 2016, 2169, 2642
FauI CCCGC 1 cut(s) 1732
FauNDI CATATG 2 cut(s) 1284, 2728
FbaI TGATCA 2 cut(s) 1192, 2698
FriOI GRGCYC 2 cut(s) 896, 982
GlaI GCGC 3 cut(s) 159, 1758, 2603
GsaI CCCAGC 3 cut(s) 288, 2919, 3228
GsuI CTGGAG 5 cut(s) 330, 1113, 1736, 2046, 2888
HaeII RGCGCY 2 cut(s) 161, 1760
HaeIII GGCC 6 cut(s) 25, 864, 894, 2468, 2592, 2669
HapII CCGG 2 cut(s) 596, 1746
HgaI GACGC 2 cut(s) 89, 324
HhaI GCGC 3 cut(s) 160, 1759, 2604
Hin1I GRCGYC 3 cut(s) 158, 1757, 1796
Hin6I GCGC 3 cut(s) 158, 1757, 2602
HinP1I GCGC 3 cut(s) 158, 1757, 2602
HincII GTYRAC 1 cut(s) 2939
HindII GTYRAC 1 cut(s) 2939
HindIII AAGCTT 1 cut(s) 2474
HpaII CCGG 2 cut(s) 596, 1746
HphI GGTGA 4 cut(s) 1187, 1911, 2536, 3254
Hpy166II GTNNAC 3 cut(s) 508, 2359, 2939
Hpy8I GTNNAC 3 cut(s) 508, 2359, 2939
Hpy99I CGWCG 2 cut(s) 105, 340
HpyCH4III ACNGT 4 cut(s) 169, 821, 1299, 1935
HpyCH4IV ACGT 3 cut(s) 1796, 2690, 3084
HpySE526I ACGT 3 cut(s) 1796, 2690, 3084
Hsp92I GRCGYC 3 cut(s) 158, 1757, 1796
HspAI GCGC 3 cut(s) 158, 1757, 2602
KasI GGCGCC 2 cut(s) 157, 1756
KpnI GGTACC 1 cut(s) 1631
Ksp22I TGATCA 2 cut(s) 1192, 2698
KspI CCGCGG 1 cut(s) 407
MaeII ACGT 3 cut(s) 1796, 2690, 3084
MaeIII GTNAC 9 cut(s) 128, 821, 1412, 1606, 1917, 1929, 2180, 2612, 2707
MflI RGATCY 4 cut(s) 536, 848, 1096, 1351
MhlI GDGCHC 5 cut(s) 896, 982, 1158, 2439, 2763
MlsI TGGCCA 2 cut(s) 864, 2468
MluNI TGGCCA 2 cut(s) 864, 2468
Mly113I GGCGCC 2 cut(s) 158, 1757
MlyI GAGTC 5 cut(s) 712, 1097, 1738, 2311, 2944
MmeI TCCRAC 2 cut(s) 318, 3081
Mox20I TGGCCA 2 cut(s) 864, 2468
Mph1103I ATGCAT 2 cut(s) 763, 3166
MroXI GAANNNNTTC 2 cut(s) 693, 1642
MscI TGGCCA 2 cut(s) 864, 2468
MseI TTAA 9 cut(s) 564, 845, 1062, 1256, 1446, 1520, 2100, 2223, 3147
MslI CAYNNNNRTG 4 cut(s) 2727, 2731, 2766, 3159
Msp20I TGGCCA 2 cut(s) 864, 2468
MspA1I CMGCKG 3 cut(s) 406, 617, 1691
MspI CCGG 2 cut(s) 596, 1746
Mva1269I GAATGC 2 cut(s) 190, 3166
MvnI CGCG 2 cut(s) 406, 3126
NarI GGCGCC 2 cut(s) 158, 1757
NcoI CCATGG 3 cut(s) 1554, 2034, 2159
NdeI CATATG 2 cut(s) 1284, 2728
NheI GCTAGC 1 cut(s) 1562
NmuCI GTSAC 6 cut(s) 128, 821, 1917, 1929, 2612, 2707
NsiI ATGCAT 2 cut(s) 763, 3166
NspI RCATGY 5 cut(s) 874, 2017, 2273, 2824, 2864
PaeI GCATGC 1 cut(s) 2824
PaeR7I CTCGAG 1 cut(s) 568
PciI ACATGT 1 cut(s) 2269
PcsI WCGNNNNNNNCGW 1 cut(s) 161
PctI GAATGC 2 cut(s) 190, 3166
PdmI GAANNNNTTC 2 cut(s) 693, 1642
Pfl23II CGTACG 1 cut(s) 713
PflMI CCANNNNNTGG 2 cut(s) 32, 889
PfoI TCCNGGA 3 cut(s) 307, 1633, 2023
PinAI ACCGGT 1 cut(s) 1745
PleI GAGTC 5 cut(s) 712, 1096, 1737, 2310, 2943
PluTI GGCGCC 2 cut(s) 161, 1760
PpsI GAGTC 5 cut(s) 712, 1096, 1737, 2310, 2943
PscI ACATGT 1 cut(s) 2269
Psp1406I AACGTT 1 cut(s) 2690
PspFI CCCAGC 3 cut(s) 284, 2915, 3224
PspLI CGTACG 1 cut(s) 713
PspOMI GGGCCC 1 cut(s) 892
PspPI GGNCC 7 cut(s) 892, 893, 1676, 1743, 2156, 2557, 2987
PspXI VCTCGAGB 1 cut(s) 568
PsrI GAACNNNNNNTAC 2 cut(s) 31, 63
PstI CTGCAG 4 cut(s) 880, 922, 1615, 1813
PstNI CAGNNNCTG 1 cut(s) 1694
PsuI RGATCY 4 cut(s) 536, 848, 1096, 1351
PvuII CAGCTG 1 cut(s) 1691
RsaI GTAC 7 cut(s) 715, 1316, 1576, 1629, 1653, 2865, 3090
RsaNI GTAC 7 cut(s) 714, 1315, 1575, 1628, 1652, 2864, 3089
RseI CAYNNNNRTG 4 cut(s) 2727, 2731, 2766, 3159
SacII CCGCGG 1 cut(s) 407
SaqAI TTAA 9 cut(s) 564, 845, 1062, 1256, 1446, 1520, 2100, 2223, 3147
Sau96I GGNCC 7 cut(s) 892, 893, 1676, 1743, 2156, 2557, 2987
ScaI AGTACT 1 cut(s) 1653
SchI GAGTC 5 cut(s) 712, 1097, 1738, 2311, 2944
SduI GDGCHC 5 cut(s) 896, 982, 1158, 2439, 2763
SfcI CTRYAG 4 cut(s) 876, 918, 1611, 1809
SfoI GGCGCC 2 cut(s) 159, 1758
Sfr274I CTCGAG 1 cut(s) 568
Sfr303I CCGCGG 1 cut(s) 407
SgrBI CCGCGG 1 cut(s) 407
SinI GGWCC 5 cut(s) 1676, 1743, 2156, 2557, 2987
SlaI CTCGAG 1 cut(s) 568
SmiMI CAYNNNNRTG 4 cut(s) 2727, 2731, 2766, 3159
SmlI CTYRAG 4 cut(s) 568, 1789, 2376, 2756
SmoI CTYRAG 4 cut(s) 568, 1789, 2376, 2756
SpeI ACTAGT 1 cut(s) 3007
SphI GCATGC 1 cut(s) 2824
SspDI GGCGCC 2 cut(s) 157, 1756
SspI AATATT 1 cut(s) 139
StyI CCWWGG 5 cut(s) 1545, 1554, 1953, 2034, 2159
TaaI ACNGT 4 cut(s) 169, 821, 1299, 1935
TaiI ACGT 3 cut(s) 1799, 2693, 3087
TaqI TCGA 5 cut(s) 386, 428, 548, 569, 1439
TatI WGTACW 3 cut(s) 1651, 2863, 3088
TauI GCSGC 3 cut(s) 163, 409, 556
Tru1I TTAA 9 cut(s) 564, 845, 1062, 1256, 1446, 1520, 2100, 2223, 3147
Tru9I TTAA 9 cut(s) 564, 845, 1062, 1256, 1446, 1520, 2100, 2223, 3147
TscAI CASTG 2 cut(s) 2290, 2768
TseFI GTSAC 6 cut(s) 128, 821, 1917, 1929, 2612, 2707
Tsp45I GTSAC 6 cut(s) 128, 821, 1917, 1929, 2612, 2707
TspGWI ACGGA 2 cut(s) 2354, 2454
TspRI CASTG 2 cut(s) 2290, 2768
Van91I CCANNNNNTGG 2 cut(s) 32, 889
VpaK11BI GGWCC 5 cut(s) 1676, 1743, 2156, 2557, 2987
XagI CCTNNNNNAGG 1 cut(s) 1005
XapI RAATTY 7 cut(s) 62, 1123, 1495, 1876, 2128, 2134, 2279
XbaI TCTAGA 1 cut(s) 1646
XceI RCATGY 5 cut(s) 874, 2017, 2273, 2824, 2864
XcmI CCANNNNNNNNNTGG 1 cut(s) 1574
XhoI CTCGAG 1 cut(s) 568
XmnI GAANNNNTTC 2 cut(s) 693, 1642
ZraI GACGTC 1 cut(s) 1797
ZrmI AGTACT 1 cut(s) 1653
Zsp2I ATGCAT 2 cut(s) 763, 3166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.