Rh5AG434700

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002776
Physical Location & Seq
Forward (+)
74758082 .. 74764866
6785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG434700.1

Sequence Viewer

Length: 3267 bp
ATGTTGTCTGAATTGGGGAGAAGGCCAATGCTTGGAGGTAACGAGGGTTCGTTTGGGGATGAATTTGAGAAGGAGATTGGTATGTTGCTTAGGGAGCAGCGTCGGCAGGAGGCTGATGATCGTGAGAGTGACCTGAATATTTACAGGAGTGGGTCGGCGCCGCCGACTGTGGAGGGTTCTTTGAATGCGGTGGGAGGGTTGTTTGCTGGCGGTGGCGGTGGAAGTGGAGGTGGAGGAGGAGGAGGAGGAGGAGGGGGTGTTGGCGGTGGTGGGGGTGCTGGGTCTGTTCTTTCGGAGTTTCCTGGAGCTAAGAATGGGAATGGGTTTGCGTCGGAGGAGGAGCTGAGGTCTGATCCAGCTTATCTGCAATACTATTACTCGAATGTGAATCTGAATCCGCGGCTTCCGCCTCCTTTGTTGTCGAAGGAGGATTGGAGGTATGCACAGAGGATGAAAGGAGGGAGTTCTGTTTTGGGTGGGATTGGGGACAGGAGGAAAGTGAACAGGGCTGATGATGCTAGTGGTGGGAGATCCATGTTTTCGATGCCGCCAGGGTTTAACTCGAGGAAACAAGAGAGTGAGGTGGAGCCGGACAAAGTGCGTGGCTCAGCGGAGTGGGGTGTTGATGGGCTAATTGGTTTGCCAGGTTTAGGACTTGGCAACAAGCAAAAAAGCCTTGCTGAAATCTTTCAGGATGACTTGGGGCGTACGACTCCTGTTCCTGGGCTTCCTTCTCGTCCAGCCAGCCGTAATGCATTTGATGAAAATGTAGAGGGACTTGGTTCTGCTGAAGCAGACCTGGCTCATTTGCGCCGTGACTTGATGACATCTGATGCTCTAAGATCTGGTGCAAATGGCCAGGGCATGTCTGCAGCCCAAAGCATGGGCCCACCTTCTTCATACTCCTATGCTGCAGCTCTAGGTGCTTCCTTGTCAAGAAGCACCACTCCTGATCCTCAGATTGTTGCTAGGGCTCCTAGTCCTTGCCTTACACCTATTGGAGGAGGAAGAGTTAGTGCTTCAGAAAAGAGAGGCATTTCTAGTCCAAGCTCCTTTAATGCTGTCTCATCTGGCATTAATGAGTCTGGAGATCTGGTAGCTGCCTTATCCACCATGAATTTGTCTTCAAATGGTGTAATAGATGATGAGCACCATTTGCCATCACAGATCAAACAGGATGTTATTGATCACCAGAATTATCTCTTTGGTCTGCAAGGTGCTGATAGTCATGCTAAGCAACTTGCTTACTTAAAGAAGTCTGAATCTGGGAATATACATATGCCTTCTCCACAGTCGGCAAAAGGTTCGTACTCGGATTTGGGTAAGAGCAATGGGGGTGGGTCAGATCTGAACAACTCATCCCCTGACAGGCAAGTTGAGCTGCAAAAATCTGCTGTTCCTTCTAGTAACTTGTATAAAGGATCATCTGCATCGAATCTTAATGGTGGAGGTGGATTGCATAATCAGTATCAGCAAGTGGATAATGCAAATTCATCATTTTCTAACTATGGTTTAAGTGGCTACTCTATGAATCCAGCCTTGGCATCCATGGTGGCTAGCCAACTTGGTACTGGTAATCTGCCTCCTCTGTTTGAAAATGTTGCTGCAGCATCAGCTATGGTACCTCCTGGAATGGATTCAAGAGTACTTGGAGGGGGTTTGGCTTCTGGACCAAACCTAGCAGCTGCTGCATCTGATTCACATAATCTTGGTAGATTAGGGAGTCCGATTGCGGGGACCGGTCTCCAGGCGCCTTATGTTGATCCTATGTATCTTCAATACTTGAGGACATCAGAGTATGCTGCAGCACAGCTTGCTGCCCTGAATGATCCTTCTGTGGATAGGAACTACTTAGGTAATTCATACATGAATTTACTTGAACTTCAAAAGGCGTATCTTGGAGCCTTGCTGTCACCTCAGAAGTCACAGTATGGTGTTGGTGTTCCCTTGGGGGGTAAGACTGGTGGTTCAAATCATCATGGTTATTATGGAAATCATGCCTTTGGCATGTCTTATCCAGGAAGTCCCATGGCAAGTCCCGTTATTCCCAACTCTCCAGTTGCCCCTGGTAGTCCAATGAGGCACAATGATCTTAATATGTGTTATCCTTCTGGGATGAGGAATTTGAATTTGGGTGGGAGTGTCATGGGACCATGGCATCTGGATTCTGGTTGTAACATAGATGAGACCTTTGCATCATCACTCTTAGAAGAGTTTAAGAGCAACAAAGCCAAGTCTTTTGAACTTTTGGAAATCGGAGGACATGTTGTGGAATTCAGTGCGGATCAATACGGGAGTCGGTTTATTCAACAAAAACTTGAAACAGCCACTACGGATGAGAAAAACATGGTTTACCAGGAAATCATGCCTCAAGCTCTTGCTCTAATGACTGATGTGTTTGGTAATTATGTGATTCAAAAGTTTTTTGAGCACGGACTTCCATCTCAAAGAAGGGAATTGGCCAACAAGCTTTTTGGTCATGTGCTGACACTTAGCCTTCAAATGTATGGTTGTCGGGTGATACAGAAGGCAATAGAAGTTGTTGATCTGGACCAGAAGATCAAAATGGTTGAAGAACTTGACGGCCATGTCATGCGCTGTGTGCGTGACCAGAATGGGAACCATGTCATTCAGAAGTGTATTGAATGTGTCCCTGAAGAGGCCATTCATTTTATAGTCTCAACATTTTTTGATCAAGTTGTGACCCTTTCCACTCATCCATATGGGTGTCGTGTGATACAGAGAGTGCTTGAGCACTGCAATGACCAGAATACTCAGAGCAAAGTTATGGATGAGATTTTAGGAGCTGTTAGCATGCTGGCACAAGATCAGTATGGCAATTATGTTGTTCAGCATGTACTGGAGCATGGAAAGCCACATGAGCGTTCTGCTATAATAAAGGAATTAGCTGGGAAGATAGTCCAAATGAGTCAACAGAAGTTTGCCTCTAATGTTGTAGAGAAATGCTTAGCTTTTGGTGGTCCTGCTGAAAGGGAATTACTAGTGAATGAGATGCTTGGCACTACTGATGAAAACGAGCCTCTTCAGGCAATGATGAAAGATCAGTTTGCAAACTACGTTGTACAGAAAGTGTTGGAGACTTGTGATGACCAACAACGCGAGCTGATTCTTTCACGAATTAAAGTTCATTTGAATGCATTGAAGAAATACACTTACGGGAAGCATATCGTGGCTCGTGTAGAAAAACTTGTTGCTGCTGGGGAAAGGAGAGTTGCTGCTGCTCAGTTGGCTCCTCACCCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1088

Amino Acids

116.03

Weight (kDa)

5.83

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 324 - 749 4.4e-172 Nucleic acid binding protein NABP
PUF PF00806 755 - 789 2.9e-10 Pumilio-family RNA binding repeat
PUF PF00806 793 - 824 1.3e-07 Pumilio-family RNA binding repeat
PUF PF00806 827 - 860 1.8e-09 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 863 - 944 1.6e-07 NOP9-like PUF repeat domain
PUF PF00806 869 - 893 1e-05 Pumilio-family RNA binding repeat
PUF PF00806 899 - 934 7.4e-13 Pumilio-family RNA binding repeat
PUF PF00806 939 - 969 8.4e-10 Pumilio-family RNA binding repeat
PUF PF00806 973 - 1006 4.2e-07 Pumilio-family RNA binding repeat
PUF PF00806 1018 - 1047 2e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2588
Acc65I GGTACC 1 cut(s) 1621
AccB1I GGYRCC 3 cut(s) 157, 1621, 1750
AccB7I CCANNNNNTGG 2 cut(s) 32, 883
AccII CGCG 2 cut(s) 400, 3120
AclWI GGATC 7 cut(s) 347, 525, 947, 1429, 1757, 1823, 2292
AcoI YGGCCR 3 cut(s) 856, 2460, 2584
AcsI RAATTY 7 cut(s) 62, 1117, 1489, 1870, 2122, 2128, 2273
AcuI CTGAAG 4 cut(s) 810, 1005, 2676, 3029
AcyI GRCGYC 2 cut(s) 158, 1751
AfaI GTAC 7 cut(s) 709, 1310, 1570, 1623, 1647, 2859, 3084
AfiI CCNNNNNNNGG 9 cut(s) 32, 650, 722, 883, 1001, 1368, 1733, 1952, 2659
AflIII ACRYGT 1 cut(s) 2263
AgeI ACCGGT 1 cut(s) 1739
AhlI ACTAGT 1 cut(s) 3001
AjuI GAANNNNNNNTTGG 4 cut(s) 1523, 1555, 2114, 2146
AloI GAACNNNNNNTCC 2 cut(s) 1343, 1375
Alw21I GWGCWC 3 cut(s) 1152, 2433, 2757
Alw26I GTCTC 5 cut(s) 1069, 1748, 2180, 2683, 3092
AlwI GGATC 7 cut(s) 347, 525, 947, 1429, 1757, 1823, 2292
AlwNI CAGNNNCTG 1 cut(s) 1688
Ama87I CYCGRG 1 cut(s) 562
AoxI GGCC 6 cut(s) 23, 856, 886, 2460, 2584, 2661
ApaI GGGCCC 1 cut(s) 890
ApoI RAATTY 7 cut(s) 62, 1117, 1489, 1870, 2122, 2128, 2273
AseI ATTAAT 1 cut(s) 1077
AsiGI ACCGGT 1 cut(s) 1739
Asp700I GAANNNNTTC 2 cut(s) 687, 1636
Asp718I GGTACC 1 cut(s) 1621
AspLEI GCGC 4 cut(s) 160, 813, 1753, 2598
AspS9I GGNCC 7 cut(s) 886, 887, 1670, 1737, 2150, 2551, 2981
AsuHPI GGTGA 4 cut(s) 1181, 1905, 2530, 3248
AsuNHI GCTAGC 1 cut(s) 1556
AvaI CYCGRG 1 cut(s) 562
AvaII GGWCC 5 cut(s) 1670, 1737, 2150, 2551, 2981
BaeGI GKGCMC 1 cut(s) 890
BalI TGGCCA 2 cut(s) 858, 2462
BanI GGYRCC 3 cut(s) 157, 1621, 1750
BanII GRGCYC 2 cut(s) 890, 976
BauI CACGAG 1 cut(s) 3195
BbsI GAAGAC 1 cut(s) 1116
Bbv12I GWGCWC 3 cut(s) 1152, 2433, 2757
BbvCI CCTCAGC 1 cut(s) 344
BccI CCATC 3 cut(s) 620, 1168, 2449
BceAI ACGGC 3 cut(s) 732, 798, 2599
BcgI CGANNNNNNTGC 2 cut(s) 1287, 1321
BclI TGATCA 2 cut(s) 1186, 2692
BcoDI GTCTC 5 cut(s) 1069, 1748, 2180, 2683, 3092
BcuI ACTAGT 1 cut(s) 3001
BfaI CTAG 9 cut(s) 519, 920, 969, 978, 1041, 1404, 1557, 1679, 3002
BfmI CTRYAG 4 cut(s) 870, 912, 1605, 1803
BfoI RGCGCY 2 cut(s) 161, 1754
BglII AGATCT 3 cut(s) 842, 1090, 1345
BlpI GCTNAGC 3 cut(s) 607, 1233, 2968
BmcAI AGTACT 1 cut(s) 1647
Bme18I GGWCC 5 cut(s) 1670, 1737, 2150, 2551, 2981
BmeT110I CYCGRG 1 cut(s) 562
BmgT120I GGNCC 7 cut(s) 886, 887, 1670, 1737, 2150, 2551, 2981
BmtI GCTAGC 1 cut(s) 1560
BpiI GAAGAC 1 cut(s) 1116
BpmI CTGGAG 5 cut(s) 324, 1107, 1730, 2040, 2882
Bpu10I CCTNAGC 2 cut(s) 89, 344
Bpu1102I GCTNAGC 3 cut(s) 607, 1233, 2968
BpuEI CTTGAG 3 cut(s) 1804, 2355, 2771
BsaHI GRCGYC 2 cut(s) 158, 1751
BsaI GGTCTC 2 cut(s) 1748, 2180
BsaWI WCCGGW 1 cut(s) 1739
Bsc4I CCNNNNNNNGG 9 cut(s) 32, 650, 722, 883, 1001, 1368, 1733, 1952, 2659
Bse118I RCCGGY 1 cut(s) 1739
Bse1I ACTGG 4 cut(s) 1576, 1966, 2057, 2865
Bse3DI GCAATG 3 cut(s) 1336, 2767, 3057
BseLI CCNNNNNNNGG 9 cut(s) 32, 650, 722, 883, 1001, 1368, 1733, 1952, 2659
BseMI GCAATG 3 cut(s) 1336, 2767, 3057
BseMII CTCAG 6 cut(s) 335, 621, 971, 1931, 2789, 3257
BseNI ACTGG 4 cut(s) 1576, 1966, 2057, 2865
BseSI GKGCMC 1 cut(s) 890
BseYI CCCAGC 3 cut(s) 278, 2909, 3218
Bsh1236I CGCG 2 cut(s) 400, 3120
BshFI GGCC 6 cut(s) 25, 858, 888, 2462, 2586, 2663
BshNI GGYRCC 3 cut(s) 157, 1621, 1750
BshTI ACCGGT 1 cut(s) 1739
BsiHKAI GWGCWC 3 cut(s) 1152, 2433, 2757
BsiHKCI CYCGRG 1 cut(s) 562
BsiSI CCGG 2 cut(s) 590, 1740
BsiWI CGTACG 1 cut(s) 707
BslFI GGGAC 7 cut(s) 500, 789, 1750, 2010, 2022, 2163, 2636
BslI CCNNNNNNNGG 9 cut(s) 32, 650, 722, 883, 1001, 1368, 1733, 1952, 2659
BsmAI GTCTC 5 cut(s) 1069, 1748, 2180, 2683, 3092
BsmFI GGGAC 7 cut(s) 500, 789, 1750, 2010, 2022, 2163, 2636
BsmI GAATGC 2 cut(s) 190, 3160
BsnI GGCC 6 cut(s) 25, 858, 888, 2462, 2586, 2663
Bso31I GGTCTC 2 cut(s) 1748, 2180
BsoBI CYCGRG 1 cut(s) 562
Bsp120I GGGCCC 1 cut(s) 886
Bsp1286I GDGCHC 5 cut(s) 890, 976, 1152, 2433, 2757
Bsp1407I TGTACA 1 cut(s) 3082
Bsp1720I GCTNAGC 3 cut(s) 607, 1233, 2968
Bsp19I CCATGG 3 cut(s) 1548, 2028, 2153
BspANI GGCC 6 cut(s) 25, 858, 888, 2462, 2586, 2663
BspCNI CTCAG 6 cut(s) 336, 620, 970, 1930, 2788, 3256
BspFNI CGCG 2 cut(s) 400, 3120
BspMAI CTGCAG 4 cut(s) 874, 916, 1609, 1807
BspOI GCTAGC 1 cut(s) 1560
BspPI GGATC 7 cut(s) 347, 525, 947, 1429, 1757, 1823, 2292
BspT107I GGYRCC 3 cut(s) 157, 1621, 1750
BspTNI GGTCTC 2 cut(s) 1748, 2180
BsrDI GCAATG 3 cut(s) 1336, 2767, 3057
BsrFI RCCGGY 1 cut(s) 1739
BsrGI TGTACA 1 cut(s) 3082
BsrI ACTGG 4 cut(s) 1576, 1966, 2057, 2865
BssAI RCCGGY 1 cut(s) 1739
BssNI GRCGYC 2 cut(s) 158, 1751
BssSI CACGAG 1 cut(s) 3195
BssT1I CCWWGG 5 cut(s) 1539, 1548, 1947, 2028, 2153
Bst2BI CACGAG 1 cut(s) 3195
Bst4CI ACNGT 3 cut(s) 169, 1293, 1929
Bst6I CTCTTC 4 cut(s) 1003, 2205, 2652, 3048
BstACI GRCGYC 2 cut(s) 158, 1751
BstAPI GCANNNNNTGC 3 cut(s) 1156, 1688, 1814
BstAUI TGTACA 1 cut(s) 3082
BstC8I GCNNGC 7 cut(s) 208, 745, 1558, 1815, 2816, 2820, 3122
BstDSI CCRYGG 4 cut(s) 398, 1548, 2028, 2153
BstENI CCTNNNNNAGG 1 cut(s) 999
BstFNI CGCG 2 cut(s) 400, 3120
BstH2I RGCGCY 2 cut(s) 161, 1754
BstHHI GCGC 4 cut(s) 160, 813, 1753, 2598
BstMAI GTCTC 5 cut(s) 1069, 1748, 2180, 2683, 3092
BstNSI RCATGY 5 cut(s) 868, 2011, 2267, 2818, 2858
BstSFI CTRYAG 4 cut(s) 870, 912, 1605, 1803
BstSLI GKGCMC 1 cut(s) 890
BstUI CGCG 2 cut(s) 400, 3120
BstV2I GAAGAC 1 cut(s) 1116
BstX2I RGATCY 4 cut(s) 530, 842, 1090, 1345
BstYI RGATCY 4 cut(s) 530, 842, 1090, 1345
BsuRI GGCC 6 cut(s) 25, 858, 888, 2462, 2586, 2663
BtgI CCRYGG 4 cut(s) 398, 1548, 2028, 2153
BtsI GCAGTG 1 cut(s) 2755
BtsIMutI CAGTG 2 cut(s) 2284, 2755
Cac8I GCNNGC 7 cut(s) 208, 745, 1558, 1815, 2816, 2820, 3122
CaiI CAGNNNCTG 1 cut(s) 1688
CfoI GCGC 4 cut(s) 160, 813, 1753, 2598
Cfr10I RCCGGY 1 cut(s) 1739
Cfr13I GGNCC 7 cut(s) 886, 887, 1670, 1737, 2150, 2551, 2981
Cfr42I CCGCGG 1 cut(s) 401
CseI GACGC 2 cut(s) 89, 318
Csp6I GTAC 7 cut(s) 708, 1309, 1569, 1622, 1646, 2858, 3083
CspAI ACCGGT 1 cut(s) 1739
CspCI CAANNNNNGTGG 4 cut(s) 583, 618, 1318, 1353
CviQI GTAC 7 cut(s) 708, 1309, 1569, 1622, 1646, 2858, 3083
DinI GGCGCC 2 cut(s) 159, 1752
DrdI GACNNNNNNGTC 1 cut(s) 2588
DseDI GACNNNNNNGTC 1 cut(s) 2588
EaeI YGGCCR 3 cut(s) 856, 2460, 2584
Eam1104I CTCTTC 4 cut(s) 1003, 2205, 2652, 3048
EarI CTCTTC 4 cut(s) 1003, 2205, 2652, 3048
EciI GGCGGA 1 cut(s) 396
Eco130I CCWWGG 5 cut(s) 1539, 1548, 1947, 2028, 2153
Eco24I GRGCYC 2 cut(s) 890, 976
Eco31I GGTCTC 2 cut(s) 1748, 2180
Eco47I GGWCC 5 cut(s) 1670, 1737, 2150, 2551, 2981
Eco57I CTGAAG 4 cut(s) 810, 1005, 2676, 3029
Eco88I CYCGRG 1 cut(s) 562
EcoNI CCTNNNNNAGG 1 cut(s) 999
EcoRI GAATTC 1 cut(s) 2273
EcoT14I CCWWGG 5 cut(s) 1539, 1548, 1947, 2028, 2153
EcoT22I ATGCAT 2 cut(s) 757, 3160
EcoT38I GRGCYC 2 cut(s) 890, 976
EgeI GGCGCC 2 cut(s) 159, 1752
EheI GGCGCC 2 cut(s) 159, 1752
ErhI CCWWGG 5 cut(s) 1539, 1548, 1947, 2028, 2153
FaqI GGGAC 7 cut(s) 500, 789, 1750, 2010, 2022, 2163, 2636
FauI CCCGC 1 cut(s) 1726
FauNDI CATATG 2 cut(s) 1278, 2722
FbaI TGATCA 2 cut(s) 1186, 2692
FriOI GRGCYC 2 cut(s) 890, 976
FspBI CTAG 9 cut(s) 519, 920, 969, 978, 1041, 1404, 1557, 1679, 3002
GlaI GCGC 4 cut(s) 159, 812, 1752, 2597
GsaI CCCAGC 3 cut(s) 282, 2913, 3222
GsuI CTGGAG 5 cut(s) 324, 1107, 1730, 2040, 2882
HaeII RGCGCY 2 cut(s) 161, 1754
HaeIII GGCC 6 cut(s) 25, 858, 888, 2462, 2586, 2663
HapII CCGG 2 cut(s) 590, 1740
HgaI GACGC 2 cut(s) 89, 318
HhaI GCGC 4 cut(s) 160, 813, 1753, 2598
Hin1I GRCGYC 2 cut(s) 158, 1751
Hin6I GCGC 4 cut(s) 158, 811, 1751, 2596
HinP1I GCGC 4 cut(s) 158, 811, 1751, 2596
HincII GTYRAC 1 cut(s) 2933
HindII GTYRAC 1 cut(s) 2933
HindIII AAGCTT 1 cut(s) 2468
HpaII CCGG 2 cut(s) 590, 1740
HphI GGTGA 4 cut(s) 1181, 1905, 2530, 3248
Hpy166II GTNNAC 3 cut(s) 502, 2353, 2933
Hpy8I GTNNAC 3 cut(s) 502, 2353, 2933
Hpy99I CGWCG 2 cut(s) 105, 334
HpyCH4III ACNGT 3 cut(s) 169, 1293, 1929
HpyCH4IV ACGT 1 cut(s) 3078
HpySE526I ACGT 1 cut(s) 3078
Hsp92I GRCGYC 2 cut(s) 158, 1751
HspAI GCGC 4 cut(s) 158, 811, 1751, 2596
KasI GGCGCC 2 cut(s) 157, 1750
KpnI GGTACC 1 cut(s) 1625
Ksp22I TGATCA 2 cut(s) 1186, 2692
KspI CCGCGG 1 cut(s) 401
MaeI CTAG 9 cut(s) 519, 920, 969, 978, 1041, 1404, 1557, 1679, 3002
MaeII ACGT 1 cut(s) 3078
MaeIII GTNAC 9 cut(s) 38, 128, 815, 1406, 1911, 1923, 2174, 2606, 2701
MflI RGATCY 4 cut(s) 530, 842, 1090, 1345
MhlI GDGCHC 5 cut(s) 890, 976, 1152, 2433, 2757
MlsI TGGCCA 2 cut(s) 858, 2462
MluNI TGGCCA 2 cut(s) 858, 2462
Mly113I GGCGCC 2 cut(s) 158, 1751
MlyI GAGTC 5 cut(s) 706, 1091, 1732, 2305, 2938
MmeI TCCRAC 2 cut(s) 312, 3075
Mox20I TGGCCA 2 cut(s) 858, 2462
Mph1103I ATGCAT 2 cut(s) 757, 3160
MroXI GAANNNNTTC 2 cut(s) 687, 1636
MscI TGGCCA 2 cut(s) 858, 2462
MseI TTAA 9 cut(s) 558, 1056, 1077, 1250, 1440, 1514, 2094, 2217, 3141
MslI CAYNNNNRTG 4 cut(s) 2721, 2725, 2760, 3153
Msp20I TGGCCA 2 cut(s) 858, 2462
MspA1I CMGCKG 3 cut(s) 400, 611, 1685
MspI CCGG 2 cut(s) 590, 1740
Mva1269I GAATGC 2 cut(s) 190, 3160
MvnI CGCG 2 cut(s) 400, 3120
NarI GGCGCC 2 cut(s) 158, 1751
NcoI CCATGG 3 cut(s) 1548, 2028, 2153
NdeI CATATG 2 cut(s) 1278, 2722
NheI GCTAGC 1 cut(s) 1556
NmuCI GTSAC 6 cut(s) 128, 815, 1911, 1923, 2606, 2701
NsiI ATGCAT 2 cut(s) 757, 3160
NspI RCATGY 5 cut(s) 868, 2011, 2267, 2818, 2858
PaeI GCATGC 1 cut(s) 2818
PaeR7I CTCGAG 1 cut(s) 562
PciI ACATGT 1 cut(s) 2263
PcsI WCGNNNNNNNCGW 1 cut(s) 161
PctI GAATGC 2 cut(s) 190, 3160
PdmI GAANNNNTTC 2 cut(s) 687, 1636
Pfl23II CGTACG 1 cut(s) 707
PflMI CCANNNNNTGG 2 cut(s) 32, 883
PfoI TCCNGGA 3 cut(s) 301, 1627, 2017
PinAI ACCGGT 1 cut(s) 1739
PleI GAGTC 5 cut(s) 706, 1090, 1731, 2304, 2937
PluTI GGCGCC 2 cut(s) 161, 1754
PpsI GAGTC 5 cut(s) 706, 1090, 1731, 2304, 2937
PscI ACATGT 1 cut(s) 2263
PshBI ATTAAT 1 cut(s) 1077
PspFI CCCAGC 3 cut(s) 278, 2909, 3218
PspLI CGTACG 1 cut(s) 707
PspOMI GGGCCC 1 cut(s) 886
PspPI GGNCC 7 cut(s) 886, 887, 1670, 1737, 2150, 2551, 2981
PspXI VCTCGAGB 1 cut(s) 562
PsrI GAACNNNNNNTAC 2 cut(s) 31, 63
PstI CTGCAG 4 cut(s) 874, 916, 1609, 1807
PstNI CAGNNNCTG 1 cut(s) 1688
PsuI RGATCY 4 cut(s) 530, 842, 1090, 1345
PvuII CAGCTG 1 cut(s) 1685
RsaI GTAC 7 cut(s) 709, 1310, 1570, 1623, 1647, 2859, 3084
RsaNI GTAC 7 cut(s) 708, 1309, 1569, 1622, 1646, 2858, 3083
RseI CAYNNNNRTG 4 cut(s) 2721, 2725, 2760, 3153
SacII CCGCGG 1 cut(s) 401
SaqAI TTAA 9 cut(s) 558, 1056, 1077, 1250, 1440, 1514, 2094, 2217, 3141
Sau96I GGNCC 7 cut(s) 886, 887, 1670, 1737, 2150, 2551, 2981
ScaI AGTACT 1 cut(s) 1647
SchI GAGTC 5 cut(s) 706, 1091, 1732, 2305, 2938
SduI GDGCHC 5 cut(s) 890, 976, 1152, 2433, 2757
SfcI CTRYAG 4 cut(s) 870, 912, 1605, 1803
SfoI GGCGCC 2 cut(s) 159, 1752
Sfr274I CTCGAG 1 cut(s) 562
Sfr303I CCGCGG 1 cut(s) 401
SgrBI CCGCGG 1 cut(s) 401
SinI GGWCC 5 cut(s) 1670, 1737, 2150, 2551, 2981
SlaI CTCGAG 1 cut(s) 562
SmiMI CAYNNNNRTG 4 cut(s) 2721, 2725, 2760, 3153
SmlI CTYRAG 4 cut(s) 562, 1783, 2370, 2750
SmoI CTYRAG 4 cut(s) 562, 1783, 2370, 2750
SpeI ACTAGT 1 cut(s) 3001
SphI GCATGC 1 cut(s) 2818
SspDI GGCGCC 2 cut(s) 157, 1750
SspI AATATT 1 cut(s) 139
SspMI CTAG 9 cut(s) 519, 920, 969, 978, 1041, 1404, 1557, 1679, 3002
StyI CCWWGG 5 cut(s) 1539, 1548, 1947, 2028, 2153
TaaI ACNGT 3 cut(s) 169, 1293, 1929
TaiI ACGT 1 cut(s) 3081
TaqI TCGA 5 cut(s) 380, 422, 542, 563, 1433
TatI WGTACW 3 cut(s) 1645, 2857, 3082
TauI GCSGC 3 cut(s) 163, 403, 550
Tru1I TTAA 9 cut(s) 558, 1056, 1077, 1250, 1440, 1514, 2094, 2217, 3141
Tru9I TTAA 9 cut(s) 558, 1056, 1077, 1250, 1440, 1514, 2094, 2217, 3141
TscAI CASTG 2 cut(s) 2284, 2762
TseFI GTSAC 6 cut(s) 128, 815, 1911, 1923, 2606, 2701
Tsp45I GTSAC 6 cut(s) 128, 815, 1911, 1923, 2606, 2701
TspGWI ACGGA 2 cut(s) 2348, 2448
TspRI CASTG 2 cut(s) 2284, 2762
Van91I CCANNNNNTGG 2 cut(s) 32, 883
VpaK11BI GGWCC 5 cut(s) 1670, 1737, 2150, 2551, 2981
VspI ATTAAT 1 cut(s) 1077
XagI CCTNNNNNAGG 1 cut(s) 999
XapI RAATTY 7 cut(s) 62, 1117, 1489, 1870, 2122, 2128, 2273
XceI RCATGY 5 cut(s) 868, 2011, 2267, 2818, 2858
XcmI CCANNNNNNNNNTGG 1 cut(s) 1568
XhoI CTCGAG 1 cut(s) 562
XmnI GAANNNNTTC 2 cut(s) 687, 1636
XspI CTAG 9 cut(s) 519, 920, 969, 978, 1041, 1404, 1557, 1679, 3002
ZrmI AGTACT 1 cut(s) 1647
Zsp2I ATGCAT 2 cut(s) 757, 3160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.