Rw1G030740

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002776
Physical Location & Seq
Forward (+)
58688524 .. 58695826
7303 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G030740.1

Sequence Viewer

Length: 3087 bp
ATGGTTACTGATACGTATTCGAAGATGATGTCCGAGATGTCAATGCGATCCATGCTTAAGAACAACGGCGATTACAGTGAAGATTTGAGCCTTCTGATTCGTCAGCAGAGGCAAGAAGTGAGTGAGCGAGAGAAGGAGCTCAATCCATACAGAAGTGGGTCGGCCCCACCAACCGTAGAAGGGTCGCTGAACGCCGTCGGCGGTCTTTTCGACGCCGTTAACGGCTTTGCGAGCGAGGAGGAGCTAAGGGCTGATCCGGCTTATCATAGCTACTACTACGCCAATGTCAATCTCAATCCGCGGCTACCGCCGCCGCTGAGGTCCAAGGAGGACTGGCGGTTTGCGCAGCGGGGCGGCGCCGGAGGAGGTTCTGGGGTCGGAGGCATTGGGGATAGGAGGAAAGGCGGGAGGGGTGGTGGTGGTGAAGGGTCGCTTTTTTCTGTGCAGCCGGAGAATGGGGCGGCGGCTAGGAATGGAGAGTGGGGCGGCGATGGGCTGATTGGGTTGCCCGGGTTGGGCCTTGGGAGTAGGCAGAAGAGTATTGCCGAGATTCTTCAGGATGATATACAGAATACAACTGGCTCAAGGCACCCTTCTCGTCCAGCTAGCCGGAATGCATTTGATGATGGTGTTGAAACTTCAGATACCCAGTATGCTCAGATGCATCGTGATTTGGCATCTTTAGATGCTTTGCGCTCTGGTGGAAATAAGCAGGGTATGTCTGCTGCCCAGAACTTTGGTTCCTCGGGCTCTCATACTTATGCTTCTGCTCTAGGTGGCTCACTGTCAAGAAGCACCACACCAGATCCTCAGCTTGTATCTAGGGCTCCTAGTCCCCGGATTCCACCAGTTGGAGGAGGAAGAGCTCCTTCCACTGATAAAAATAGTGTCGGTGGTCAAAATTCATATAATGGTATCGCATCCAATGTGAAAGAGTCTGCAGATCTGGTAGCTGCCTTATCTGGTATGAACCTGTCAAAAAACAGTAGGATGCATGAAGAGAATCATGCACATTCACAGATTCAAGGTGATAACCACTTTGATATGCAAGGGGATCGGAACCATATTAAACAAAATTCATATATGAACAAGCCTGATTCAGGGAATTATCATTTGCATTCTGTTTCCCAGTCTACCAAAAACTCTTATCAGAATATGGCCAAAGGCAGTGGATTTGGGAGGGACCAGAACAACCCTTCACTGATGTCTGATGGGCTAGCTGAAATTAACAATCCTGCTGTATCTTCTGCTAACTCATACTTGAGAGGACCCTCTGCCCCAGCTCTTAATGGCCGAGGAAGCTCAGTCTCTCACTATCAGAGTGTAGACGGTATGAATTCCTCATTTGCAAACTATGGGTTAGCTGGGTATTCTGTTAGTCCATCGTCACCCTCCATGATGGGGAGCCCACTGGGTAATGGTAATTTACCACCCTTGTTTGAAAATGCTGCTGCTGTTTCTGCAATGGGTGGCTTAGACTCTGGAGCCTTTGGAGGAGGTATGTCTCTGGGACCAAATTTGTTAGCAGCAGCTGCTGAATTGCAGAATATGGGGAGGGGTGGAAATCATGCTGCCGGAGGGGCTCTTCAGATGCCCCTGATGGACCCCTTGTATCTGCAGTATTTAAGATCAAATGAGTATGCTGCTGCTGCACAGCTTGCATCCCTTCATGACCCCACAGCAGACAGGGAAGGCATGTATGTGGACTTTCTGGGGTTGCAAAAAGCTTATCTGGGACAATTACTTTCGCCTCAAAAATCACAGTTTGGTGCTCCCTACATGGGTAAGTCTGGTAGCTTGAATCATGGTTATTATGGAAATCCAGCATTTGGGCTTGGCATGTCTTATTCTGCAAGTCCCCTTCTTCCAAACTCTCCCGTTGGACCAGGTAGTCCTGTCAGACACAGTGACAGGAACTTGCGTTTTTCTTCGGGGATGAGGAACATGAGTGGAGGTCTCATGGGAGCTTGGCACTCTGAAACTGGTGGAAATTTCGATGAAAGTTTTGCATCCTCTTTGCTAGATGAGTTCAAGAGCAATAAGACTAAATGTTTTGAACTTTCAGAAATTTCAGGCCATGTTGTTGAGTTCAGTGCGGACCAGTATGGGAGTAGGTTCATTCAACAGAAACTTGAAACTGCCACAACCGAAGAAAAGAACATGGTTTTTGATGAAATTATGCCCCAAGCCCTTTCTTTGATGACTGATGTGTTTGGTAATTATGTGATTCAGAAGTTTTTCGAGCACGGTTCTGCAGCACAAATTAGAGAACTGGCTGACCAGCTCACTGGGCATGTGCTGACCCTTAGCCTTCAAATGTATGGCTGTCGAGTGATCCAGAAGGCTATAGAAGTTGTTGATCTGGACCAGCAGACTCAAATGGTCACAGAACTGGATGGTCACATTATGCGTTGTGTTCGTGATCAAAATGGGAACCATGTCATCCAGAAGTGTATTGAATGTATACCTGAAGATGCTATCCAGTTTGTTGTTTCAACCTTTTATGATCAAGTTGTGACACTGTCAACTCATCCATATGGTTGTCGTGTCATACAGAGAATTTTGGAGCACTGCCATGATCCCAAAACGCAGCAGATAATGATGGATGAGATTTTGCATGCTGTTTGCACTTTGGCACAAGATCAGTATGGAAATTATGTTGTGCAGCATGTGCTGGAGCATGGAAAGCCAGACGAGCGCTCAGATATAATTAGGAAACTAACTGGGCAGATTGTTCAAATGAGCCAGCAGAAATTTGCCTCCAATGTTATAGAGAAGTGTTTAACTTTTGGAACTCTTGCTGAGCGCCAGGCCCTTGTCACTGAGATGCTCGGTACCACTGATGAAAACGAACCCCTTCAGGCAATGATGAAGGACCAGTTCGCAAATTATGTTGTACAGAAAGTTTTGGAAACTTGTGATGACCAGCAACTTGAACTAATCCTTAACCGTATTAAGGTTCATCTGAATGCTCTGAAGAAGTATACTTATGGGAAGCATATAGTTGCACGTGTAGAGAAACTTGTTGCTGCTGGAGAGAAGAGGATTAGCATCCTGACTCCACAATGTCCACCTGCTACTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1028

Amino Acids

111.04

Weight (kDa)

5.94

Isoelectric Point (pI)

48.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 275 - 688 1.9e-147 Nucleic acid binding protein NABP
PUF PF00806 693 - 727 8e-12 Pumilio-family RNA binding repeat
PUF PF00806 730 - 762 4.7e-08 Pumilio-family RNA binding repeat
PUF PF00806 765 - 798 1.9e-09 Pumilio-family RNA binding repeat
PUF PF00806 837 - 872 2.4e-13 Pumilio-family RNA binding repeat
PUF PF00806 875 - 907 1.4e-09 Pumilio-family RNA binding repeat
PUF PF00806 910 - 943 2.1e-07 Pumilio-family RNA binding repeat
PUF PF00806 956 - 985 9.4e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3082
AasI GACNNNNNNGTC 1 cut(s) 1890
Acc16I TGCGCA 1 cut(s) 345
Acc36I ACCTGC 1 cut(s) 3082
Acc65I GGTACC 1 cut(s) 2834
AccB1I GGYRCC 3 cut(s) 356, 588, 2834
AccB7I CCANNNNNTGG 2 cut(s) 851, 1829
AccI GTMKAC 4 cut(s) 1133, 1326, 2464, 2984
AccII CGCG 1 cut(s) 301
AclWI GGATC 6 cut(s) 42, 248, 800, 1062, 2329, 2573
AcoI YGGCCR 2 cut(s) 1158, 1291
AcsI RAATTY 8 cut(s) 901, 1075, 1336, 1516, 1990, 2067, 2559, 2753
AcuI CTGAAG 6 cut(s) 539, 624, 1571, 2490, 2843, 2996
AcvI CACGTG 1 cut(s) 3011
AcyI GRCGYC 2 cut(s) 213, 357
AfaI GTAC 2 cut(s) 2836, 2898
AfeI AGCGCT 1 cut(s) 2699
AflII CTTAAG 1 cut(s) 56
AflIII ACRYGT 1 cut(s) 3010
AjnI CCWGG 2 cut(s) 1885, 2808
AloI GAACNNNNNNTCC 2 cut(s) 725, 757
Alw21I GWGCWC 5 cut(s) 141, 868, 1774, 2247, 2571
Alw26I GTCTC 3 cut(s) 1312, 1509, 1961
AlwI GGATC 6 cut(s) 42, 248, 800, 1062, 2329, 2573
AlwNI CAGNNNCTG 2 cut(s) 1532, 1535
Ama87I CYCGRG 2 cut(s) 509, 745
Aor51HI AGCGCT 1 cut(s) 2699
AoxI GGCC 6 cut(s) 162, 517, 1158, 1291, 2074, 2811
ApoI RAATTY 8 cut(s) 901, 1075, 1336, 1516, 1990, 2067, 2559, 2753
ArsI GACNNNNNNTTYG 4 cut(s) 14, 46, 323, 355
Asp700I GAANNNNTTC 2 cut(s) 2237, 2856
Asp718I GGTACC 1 cut(s) 2834
AspLEI GCGC 5 cut(s) 346, 359, 696, 2700, 2808
AsuC2I CCSGG 3 cut(s) 510, 511, 838
AsuHPI GGTGA 3 cut(s) 434, 1040, 1380
AsuII TTCGAA 1 cut(s) 20
AsuNHI GCTAGC 2 cut(s) 605, 1216
AvaI CYCGRG 2 cut(s) 509, 745
AvaII GGWCC 9 cut(s) 321, 1183, 1268, 1511, 1603, 1883, 2098, 2365, 2875
BalI TGGCCA 1 cut(s) 1160
BanI GGYRCC 3 cut(s) 356, 588, 2834
BanII GRGCYC 6 cut(s) 141, 752, 829, 868, 1409, 1585
BbrPI CACGTG 1 cut(s) 3011
Bbv12I GWGCWC 5 cut(s) 141, 868, 1774, 2247, 2571
BbvCI CCTCAGC 2 cut(s) 317, 810
BccI CCATC 8 cut(s) 485, 620, 1205, 1390, 1393, 1594, 2390, 2596
BceAI ACGGC 4 cut(s) 82, 179, 200, 238
BcgI CGANNNNNNTGC 4 cut(s) 578, 612, 1037, 1071
BciT130I CCWGG 2 cut(s) 1887, 2810
BclI TGATCA 2 cut(s) 2422, 2506
BcnI CCSGG 3 cut(s) 510, 511, 838
BcoDI GTCTC 3 cut(s) 1312, 1509, 1961
BfaI CTAG 7 cut(s) 468, 606, 773, 822, 831, 1217, 2021
BfmI CTRYAG 4 cut(s) 939, 1616, 2253, 2346
BfoI RGCGCY 3 cut(s) 360, 2701, 2809
BfrI CTTAAG 1 cut(s) 56
BfuAI ACCTGC 1 cut(s) 3082
BglI GCCNNNNNGGC 1 cut(s) 1580
BglII AGATCT 1 cut(s) 943
BlpI GCTNAGC 1 cut(s) 2802
Bme1390I CCNGG 5 cut(s) 510, 511, 838, 1887, 2810
Bme18I GGWCC 9 cut(s) 321, 1183, 1268, 1511, 1603, 1883, 2098, 2365, 2875
BmeT110I CYCGRG 2 cut(s) 509, 745
BmrFI CCNGG 5 cut(s) 510, 511, 838, 1887, 2810
BmrI ACTGGG 5 cut(s) 643, 1123, 1421, 2298, 2733
BmtI GCTAGC 2 cut(s) 609, 1220
BmuI ACTGGG 5 cut(s) 643, 1123, 1421, 2298, 2733
BpmI CTGGAG 3 cut(s) 1503, 2696, 3054
Bpu10I CCTNAGC 4 cut(s) 245, 317, 810, 2306
Bpu1102I GCTNAGC 1 cut(s) 2802
Bpu14I TTCGAA 1 cut(s) 20
BpuEI CTTGAG 2 cut(s) 568, 1282
BpuMI CCSGG 3 cut(s) 510, 511, 838
BsaAI YACGTR 2 cut(s) 15, 3011
BsaBI GATNNNNATC 1 cut(s) 3050
BsaHI GRCGYC 2 cut(s) 213, 357
BsaI GGTCTC 1 cut(s) 1961
BsaJI CCNNGG 7 cut(s) 299, 324, 509, 520, 744, 836, 1294
BsaXI ACNNNNNCTCC 2 cut(s) 400, 430
Bse3DI GCAATG 2 cut(s) 1470, 2871
Bse8I GATNNNNATC 1 cut(s) 3050
BseBI CCWGG 2 cut(s) 1887, 2810
BseDI CCNNGG 7 cut(s) 299, 324, 509, 520, 744, 836, 1294
BseJI GATNNNNATC 1 cut(s) 3050
BseMI GCAATG 2 cut(s) 1470, 2871
BseMII CTCAG 7 cut(s) 308, 671, 824, 1317, 2715, 2793, 2814
BseRI GAGGAG 5 cut(s) 251, 254, 378, 870, 1509
BseYI CCCAGC 2 cut(s) 1279, 1364
BsgI GTGCAG 3 cut(s) 464, 1635, 2684
Bsh1236I CGCG 1 cut(s) 301
BshFI GGCC 6 cut(s) 164, 519, 1160, 1293, 2076, 2813
BshNI GGYRCC 3 cut(s) 356, 588, 2834
BsiHKAI GWGCWC 5 cut(s) 141, 868, 1774, 2247, 2571
BsiHKCI CYCGRG 2 cut(s) 509, 745
BsiSI CCGG 7 cut(s) 257, 360, 449, 510, 610, 838, 1575
BslFI GGGAC 5 cut(s) 819, 1196, 1524, 1749, 1842
BsmAI GTCTC 3 cut(s) 1312, 1509, 1961
BsmFI GGGAC 5 cut(s) 819, 1196, 1524, 1749, 1842
BsmI GAATGC 3 cut(s) 619, 1117, 2974
BsnI GGCC 6 cut(s) 164, 519, 1160, 1293, 2076, 2813
Bso31I GGTCTC 1 cut(s) 1961
BsoBI CYCGRG 2 cut(s) 509, 745
Bsp119I TTCGAA 1 cut(s) 20
Bsp1286I GDGCHC 9 cut(s) 141, 752, 829, 868, 1409, 1585, 1774, 2247, 2571
Bsp1407I TGTACA 1 cut(s) 2896
Bsp1720I GCTNAGC 1 cut(s) 2802
BspANI GGCC 6 cut(s) 164, 519, 1160, 1293, 2076, 2813
BspCNI CTCAG 7 cut(s) 309, 670, 823, 1316, 2714, 2794, 2815
BspFNI CGCG 1 cut(s) 301
BspHI TCATGA 1 cut(s) 1669
BspMAI CTGCAG 3 cut(s) 943, 1620, 2257
BspMI ACCTGC 1 cut(s) 3082
BspOI GCTAGC 2 cut(s) 609, 1220
BspPI GGATC 6 cut(s) 42, 248, 800, 1062, 2329, 2573
BspQI GCTCTTC 2 cut(s) 856, 1590
BspT104I TTCGAA 1 cut(s) 20
BspT107I GGYRCC 3 cut(s) 356, 588, 2834
BspTI CTTAAG 1 cut(s) 56
BspTNI GGTCTC 1 cut(s) 1961
BsrDI GCAATG 2 cut(s) 1470, 2871
BsrGI TGTACA 1 cut(s) 2896
BssECI CCNNGG 7 cut(s) 299, 324, 509, 520, 744, 836, 1294
BssNAI GTATAC 2 cut(s) 2465, 2985
BssNI GRCGYC 2 cut(s) 213, 357
BssT1I CCWWGG 2 cut(s) 324, 520
Bst1107I GTATAC 2 cut(s) 2465, 2985
Bst2UI CCWGG 2 cut(s) 1887, 2810
Bst6I CTCTTC 5 cut(s) 530, 856, 993, 1590, 3035
BstACI GRCGYC 2 cut(s) 213, 357
BstAFI CTTAAG 1 cut(s) 56
BstAPI GCANNNNNTGC 3 cut(s) 1532, 1658, 2671
BstAUI TGTACA 1 cut(s) 2896
BstBAI YACGTR 2 cut(s) 15, 3011
BstBI TTCGAA 1 cut(s) 20
BstC8I GCNNGC 6 cut(s) 232, 607, 1218, 1659, 2619, 2747
BstDSI CCRYGG 1 cut(s) 299
BstENI CCTNNNNNAGG 1 cut(s) 1098
BstFNI CGCG 1 cut(s) 301
BstH2I RGCGCY 3 cut(s) 360, 2701, 2809
BstHHI GCGC 5 cut(s) 346, 359, 696, 2700, 2808
BstMAI GTCTC 3 cut(s) 1312, 1509, 1961
BstNI CCWGG 2 cut(s) 1887, 2810
BstNSI RCATGY 5 cut(s) 1699, 1843, 2297, 2621, 2672
BstSCI CCNGG 5 cut(s) 508, 509, 836, 1885, 2808
BstSFI CTRYAG 4 cut(s) 939, 1616, 2253, 2346
BstSNI TACGTA 1 cut(s) 15
BstUI CGCG 1 cut(s) 301
BstX2I RGATCY 2 cut(s) 805, 943
BstXI CCANNNNNNTGG 2 cut(s) 737, 2288
BstYI RGATCY 2 cut(s) 805, 943
BstZ17I GTATAC 2 cut(s) 2465, 2985
BsuRI GGCC 6 cut(s) 164, 519, 1160, 1293, 2076, 2813
BtgI CCRYGG 1 cut(s) 299
BtgZI GCGATG 1 cut(s) 504
BtsI GCAGTG 2 cut(s) 1174, 2569
BveI ACCTGC 1 cut(s) 3082
Cac8I GCNNGC 6 cut(s) 232, 607, 1218, 1659, 2619, 2747
CaiI CAGNNNCTG 2 cut(s) 1532, 1535
CciI TCATGA 1 cut(s) 1669
CfoI GCGC 5 cut(s) 346, 359, 696, 2700, 2808
Cfr42I CCGCGG 1 cut(s) 302
Cfr9I CCCGGG 1 cut(s) 509
CseI GACGC 1 cut(s) 221
CsiI ACCWGGT 1 cut(s) 1885
Csp6I GTAC 2 cut(s) 2835, 2897
CspCI CAANNNNNGTGG 2 cut(s) 1150, 1185
CviQI GTAC 2 cut(s) 2835, 2897
DinI GGCGCC 1 cut(s) 358
DrdI GACNNNNNNGTC 1 cut(s) 1890
DseDI GACNNNNNNGTC 1 cut(s) 1890
EaeI YGGCCR 2 cut(s) 1158, 1291
Eam1104I CTCTTC 5 cut(s) 530, 856, 993, 1590, 3035
EarI CTCTTC 5 cut(s) 530, 856, 993, 1590, 3035
Ecl136II GAGCTC 2 cut(s) 139, 866
Eco105I TACGTA 1 cut(s) 15
Eco130I CCWWGG 2 cut(s) 324, 520
Eco24I GRGCYC 6 cut(s) 141, 752, 829, 868, 1409, 1585
Eco31I GGTCTC 1 cut(s) 1961
Eco47I GGWCC 9 cut(s) 321, 1183, 1268, 1511, 1603, 1883, 2098, 2365, 2875
Eco47III AGCGCT 1 cut(s) 2699
Eco53kI GAGCTC 2 cut(s) 139, 866
Eco57I CTGAAG 6 cut(s) 539, 624, 1571, 2490, 2843, 2996
Eco72I CACGTG 1 cut(s) 3011
Eco88I CYCGRG 2 cut(s) 509, 745
EcoICRI GAGCTC 2 cut(s) 139, 866
EcoNI CCTNNNNNAGG 1 cut(s) 1098
EcoO109I RGGNCCY 2 cut(s) 1268, 2812
EcoRI GAATTC 1 cut(s) 1336
EcoRII CCWGG 2 cut(s) 1885, 2808
EcoT14I CCWWGG 2 cut(s) 324, 520
EcoT22I ATGCAT 3 cut(s) 619, 666, 996
EcoT38I GRGCYC 6 cut(s) 141, 752, 829, 868, 1409, 1585
EgeI GGCGCC 1 cut(s) 358
EheI GGCGCC 1 cut(s) 358
ErhI CCWWGG 2 cut(s) 324, 520
FalI AAGNNNNNCTT 6 cut(s) 417, 449, 577, 609, 853, 885
FaqI GGGAC 5 cut(s) 819, 1196, 1524, 1749, 1842
FauI CCCGC 2 cut(s) 342, 398
FauNDI CATATG 1 cut(s) 2536
FbaI TGATCA 2 cut(s) 2422, 2506
FblI GTMKAC 4 cut(s) 1133, 1326, 2464, 2984
FriOI GRGCYC 6 cut(s) 141, 752, 829, 868, 1409, 1585
FspBI CTAG 7 cut(s) 468, 606, 773, 822, 831, 1217, 2021
FspI TGCGCA 1 cut(s) 345
GlaI GCGC 5 cut(s) 345, 358, 695, 2699, 2807
GsaI CCCAGC 2 cut(s) 1283, 1368
GsuI CTGGAG 3 cut(s) 1503, 2696, 3054
HaeII RGCGCY 3 cut(s) 360, 2701, 2809
HaeIII GGCC 6 cut(s) 164, 519, 1160, 1293, 2076, 2813
HapII CCGG 7 cut(s) 257, 360, 449, 510, 610, 838, 1575
HgaI GACGC 1 cut(s) 221
HhaI GCGC 5 cut(s) 346, 359, 696, 2700, 2808
Hin1I GRCGYC 2 cut(s) 213, 357
Hin6I GCGC 5 cut(s) 344, 357, 694, 2698, 2806
HinP1I GCGC 5 cut(s) 344, 357, 694, 2698, 2806
HincII GTYRAC 2 cut(s) 220, 2526
HindII GTYRAC 2 cut(s) 220, 2526
HindIII AAGCTT 1 cut(s) 1725
HpaI GTTAAC 1 cut(s) 220
HpaII CCGG 7 cut(s) 257, 360, 449, 510, 610, 838, 1575
HphI GGTGA 3 cut(s) 434, 1040, 1380
Hpy166II GTNNAC 8 cut(s) 220, 1134, 1327, 1705, 2465, 2526, 2985, 3071
Hpy8I GTNNAC 8 cut(s) 220, 1134, 1327, 1705, 2465, 2526, 2985, 3071
Hpy99I CGWCG 2 cut(s) 200, 215
HpyCH4IV ACGT 2 cut(s) 14, 3010
HpySE526I ACGT 2 cut(s) 14, 3010
Hsp92I GRCGYC 2 cut(s) 213, 357
HspAI GCGC 5 cut(s) 344, 357, 694, 2698, 2806
KasI GGCGCC 1 cut(s) 356
KpnI GGTACC 1 cut(s) 2838
Ksp22I TGATCA 2 cut(s) 2422, 2506
KspAI GTTAAC 1 cut(s) 220
KspI CCGCGG 1 cut(s) 302
LguI GCTCTTC 2 cut(s) 856, 1590
MabI ACCWGGT 1 cut(s) 1885
MaeI CTAG 7 cut(s) 468, 606, 773, 822, 831, 1217, 2021
MaeII ACGT 2 cut(s) 14, 3010
MaeIII GTNAC 7 cut(s) 4, 1386, 1907, 2383, 2399, 2515, 2818
MflI RGATCY 2 cut(s) 805, 943
MhlI GDGCHC 9 cut(s) 141, 752, 829, 868, 1409, 1585, 1774, 2247, 2571
MlsI TGGCCA 1 cut(s) 1160
MluNI TGGCCA 1 cut(s) 1160
Mly113I GGCGCC 1 cut(s) 357
MlyI GAGTC 4 cut(s) 944, 1472, 2368, 3052
MmeI TCCRAC 3 cut(s) 358, 832, 1861
Mox20I TGGCCA 1 cut(s) 1160
Mph1103I ATGCAT 3 cut(s) 619, 666, 996
MroXI GAANNNNTTC 2 cut(s) 2237, 2856
MscI TGGCCA 1 cut(s) 1160
MseI TTAA 9 cut(s) 57, 219, 1068, 1227, 1287, 1625, 2783, 2946, 2955
MslI CAYNNNNRTG 6 cut(s) 759, 1700, 2535, 2574, 2825, 2967
Msp20I TGGCCA 1 cut(s) 1160
MspA1I CMGCKG 4 cut(s) 301, 316, 349, 1532
MspCI CTTAAG 1 cut(s) 56
MspI CCGG 7 cut(s) 257, 360, 449, 510, 610, 838, 1575
MspR9I CCNGG 5 cut(s) 510, 511, 838, 1887, 2810
Mva1269I GAATGC 3 cut(s) 619, 1117, 2974
MvaI CCWGG 2 cut(s) 1887, 2810
MvnI CGCG 1 cut(s) 301
NarI GGCGCC 1 cut(s) 357
NciI CCSGG 3 cut(s) 510, 511, 838
NdeI CATATG 1 cut(s) 2536
NheI GCTAGC 2 cut(s) 605, 1216
NmeAIII GCCGAG 2 cut(s) 571, 1319
NmuCI GTSAC 6 cut(s) 1386, 1907, 2383, 2399, 2515, 2818
NsbI TGCGCA 1 cut(s) 345
NsiI ATGCAT 3 cut(s) 619, 666, 996
NspI RCATGY 5 cut(s) 1699, 1843, 2297, 2621, 2672
NspV TTCGAA 1 cut(s) 20
PaeI GCATGC 1 cut(s) 2621
PagI TCATGA 1 cut(s) 1669
PaqCI CACCTGC 1 cut(s) 3082
PciSI GCTCTTC 2 cut(s) 856, 1590
PctI GAATGC 3 cut(s) 619, 1117, 2974
PdmI GAANNNNTTC 2 cut(s) 2237, 2856
PfeI GAWTC 8 cut(s) 97, 550, 841, 1003, 1021, 1097, 1801, 2227
PflFI GACNNNGTC 1 cut(s) 2521
PflMI CCANNNNNTGG 2 cut(s) 851, 1829
PleI GAGTC 4 cut(s) 943, 1472, 2368, 3052
PluTI GGCGCC 1 cut(s) 360
PmaCI CACGTG 1 cut(s) 3011
PmlI CACGTG 1 cut(s) 3011
PpsI GAGTC 4 cut(s) 943, 1472, 2368, 3052
Ppu21I YACGTR 2 cut(s) 15, 3011
PpuMI RGGWCCY 1 cut(s) 1268
Psp124BI GAGCTC 2 cut(s) 141, 868
Psp5II RGGWCCY 1 cut(s) 1268
Psp6I CCWGG 2 cut(s) 1885, 2808
PspCI CACGTG 1 cut(s) 3011
PspFI CCCAGC 2 cut(s) 1279, 1364
PspGI CCWGG 2 cut(s) 1885, 2808
PspPPI RGGWCCY 1 cut(s) 1268
PstI CTGCAG 3 cut(s) 943, 1620, 2257
PstNI CAGNNNCTG 2 cut(s) 1532, 1535
PsuI RGATCY 2 cut(s) 805, 943
PsyI GACNNNGTC 1 cut(s) 2521
PvuII CAGCTG 1 cut(s) 1532
RsaI GTAC 2 cut(s) 2836, 2898
RsaNI GTAC 2 cut(s) 2835, 2897
RseI CAYNNNNRTG 6 cut(s) 759, 1700, 2535, 2574, 2825, 2967
SacI GAGCTC 2 cut(s) 141, 868
SacII CCGCGG 1 cut(s) 302
SapI GCTCTTC 2 cut(s) 856, 1590
SaqAI TTAA 9 cut(s) 57, 219, 1068, 1227, 1287, 1625, 2783, 2946, 2955
SchI GAGTC 4 cut(s) 944, 1472, 2368, 3052
ScrFI CCNGG 5 cut(s) 510, 511, 838, 1887, 2810
SduI GDGCHC 9 cut(s) 141, 752, 829, 868, 1409, 1585, 1774, 2247, 2571
SexAI ACCWGGT 1 cut(s) 1885
SfcI CTRYAG 4 cut(s) 939, 1616, 2253, 2346
SfoI GGCGCC 1 cut(s) 358
Sfr303I CCGCGG 1 cut(s) 302
SfuI TTCGAA 1 cut(s) 20
SgrBI CCGCGG 1 cut(s) 302
SinI GGWCC 9 cut(s) 321, 1183, 1268, 1511, 1603, 1883, 2098, 2365, 2875
SmaI CCCGGG 1 cut(s) 511
SmiMI CAYNNNNRTG 6 cut(s) 759, 1700, 2535, 2574, 2825, 2967
SmlI CTYRAG 3 cut(s) 56, 583, 1261
SmoI CTYRAG 3 cut(s) 56, 583, 1261
SnaBI TACGTA 1 cut(s) 15
SphI GCATGC 1 cut(s) 2621
SspDI GGCGCC 1 cut(s) 356
SspMI CTAG 7 cut(s) 468, 606, 773, 822, 831, 1217, 2021
SstI GAGCTC 2 cut(s) 141, 868
StyD4I CCNGG 5 cut(s) 508, 509, 836, 1885, 2808
StyI CCWWGG 2 cut(s) 324, 520
TaiI ACGT 2 cut(s) 17, 3013
TaqI TCGA 5 cut(s) 20, 210, 1995, 2241, 2329
TatI WGTACW 1 cut(s) 2896
TauI GCSGC 7 cut(s) 304, 313, 316, 357, 464, 467, 489
TfiI GAWTC 8 cut(s) 97, 550, 841, 1003, 1021, 1097, 1801, 2227
Tru1I TTAA 9 cut(s) 57, 219, 1068, 1227, 1287, 1625, 2783, 2946, 2955
Tru9I TTAA 9 cut(s) 57, 219, 1068, 1227, 1287, 1625, 2783, 2946, 2955
TseFI GTSAC 6 cut(s) 1386, 1907, 2383, 2399, 2515, 2818
Tsp45I GTSAC 6 cut(s) 1386, 1907, 2383, 2399, 2515, 2818
TspMI CCCGGG 1 cut(s) 509
Tth111I GACNNNGTC 1 cut(s) 2521
Van91I CCANNNNNTGG 2 cut(s) 851, 1829
Vha464I CTTAAG 1 cut(s) 56
VpaK11BI GGWCC 9 cut(s) 321, 1183, 1268, 1511, 1603, 1883, 2098, 2365, 2875
XagI CCTNNNNNAGG 1 cut(s) 1098
XapI RAATTY 8 cut(s) 901, 1075, 1336, 1516, 1990, 2067, 2559, 2753
XceI RCATGY 5 cut(s) 1699, 1843, 2297, 2621, 2672
XmaI CCCGGG 1 cut(s) 509
XmiI GTMKAC 4 cut(s) 1133, 1326, 2464, 2984
XmnI GAANNNNTTC 2 cut(s) 2237, 2856
XspI CTAG 7 cut(s) 468, 606, 773, 822, 831, 1217, 2021
Zsp2I ATGCAT 3 cut(s) 619, 666, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.