pycom01g15770

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002776
Physical Location & Seq
Forward (+)
15553681 .. 15555709
2029 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g15770.1

Sequence Viewer

Length: 1245 bp
ATGGTCACCGATACTTATTCAAAGATGATGTCTGAAATGTCAATGCGATCGATGTTAAAGAGTGGTGATTACAGCGAAGATTTGAGTATGTTGAGTCGTGAACAGAGGCGGCAGCATGAAGCAAGTGAGCGGGAGAAGGAGCTGAACATATACAGGAGTGGGTCGGCGCCGCCCACCGTGGAAGGCTCGTTGAACGCTGTCGGAGGCTTGTTTGAGGCGTCTGCGCTGTCGGGTTTCACGAAAAACGGCACCAAGGGGTTTGCAACTGAAGAGGAGCTTCTCGCTGATCCAGCTTATGTGAATTACTACTATTCCAATGGTAATCTCAATCCACGGCTTCCGCCGCCGCTGCTTTCGAAGGAGGACTGGCGTTTTTCCCAGCGGTTGCAAGGCGGAGGTGGAGGTGGAGGTGGAGGTTCGGCAGTTGGAGATAGGAGGATAGGGGGTAGGAGTGGCGGTGAAGGGTCACTTTTTTCTGTGCAGCCGGGGATTGGAGGGAAGGAGGAGAATGGTGTGCCGGCGAGGAAGGGTGCGGCAGAGTGGGGCGGTGATGGGTTGATTGGGTTGCCGGGGTTGGGACTTGGGAGTAGGCAGAAGAGTATTGCAGAGATTATTCAGGATGATATACATAATACAAATGGCTCAAGGCACCCTTCTCGTCCAGCTAGCCGGAATGCTTTTGATGATGGTGTTGAAGCTTCAGATACCCAGTTTGCTCATTTACAGGGAATGTCAGCTGTTCAGAACGGCGGTTCATCTAGTTCTCATACATATGCTTCTGCTCTGGGTGCTTCATTATCGAGAAGCAACACACCAGACCCTCAGCTTGTAGCTAGGGCTCCTAGTCCTCGAATTCCACCAGTTGGAAGGGTTTCCTCCATGGATAAAAAAATTGGCAATGGTCAAAACTCATTCAATGGTGCCTCGCTTGATGTGAATGAGTCTGCAGATCTGGCAGCTGCATTATCTGGTATGAACCTGTCAACAAATGGTAGGATTGATGAGAAGAATCGTGCACGCTCACAAATGCAACATGAAATTGATAATCACCATAACCTTTATGACATGCAAGGTGATTGGAACCATATTAAACAAAATTCGTACTTGAATAAGTCTGATTCTGGGAATTTTCATCTGCATTCTGCTTCCCAATCTTCTAACAATTCTTATCAGAATATGGGAAGAGGTAGTGGATTTGGTAGGGATCTGAACAGCCCTTCTTATATGTCTGATCGATCTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

415

Amino Acids

44.16

Weight (kDa)

6.27

Isoelectric Point (pI)

53.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 279 - 413 1.2e-33 Nucleic acid binding protein NABP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 166, 248, 648, 920
AccB7I CCANNNNNTGG 1 cut(s) 863
AccBSI CCGCTC 1 cut(s) 130
AclWI GGATC 2 cut(s) 281, 1212
AcsI RAATTY 3 cut(s) 852, 1096, 1126
AcuI CTGAAG 2 cut(s) 288, 684
AcyI GRCGYC 2 cut(s) 167, 218
AfaI GTAC 1 cut(s) 1103
AfiI CCNNNNNNNGG 3 cut(s) 491, 575, 863
AgsI TTSAA 5 cut(s) 21, 193, 695, 916, 1108
AluBI AGCT 9 cut(s) 142, 277, 293, 665, 698, 737, 826, 833, 959
AluI AGCT 9 cut(s) 142, 277, 293, 665, 698, 737, 826, 833, 959
Alw21I GWGCWC 1 cut(s) 1018
Alw44I GTGCAC 1 cut(s) 1014
AlwI GGATC 2 cut(s) 281, 1212
ApaLI GTGCAC 1 cut(s) 1014
ApeKI GCWGC 5 cut(s) 112, 349, 481, 956, 959
ApoI RAATTY 3 cut(s) 852, 1096, 1126
ArsI GACNNNNNNTTYG 2 cut(s) 14, 46
Asp700I GAANNNNTTC 1 cut(s) 871
AspLEI GCGC 2 cut(s) 169, 226
AsuC2I CCSGG 2 cut(s) 486, 570
AsuHPI GGTGA 5 cut(s) 77, 470, 560, 1040, 1085
AsuII TTCGAA 1 cut(s) 356
AsuNHI GCTAGC 1 cut(s) 665
BaeGI GKGCMC 1 cut(s) 1018
BanI GGYRCC 4 cut(s) 166, 248, 648, 920
BanII GRGCYC 1 cut(s) 841
Bbv12I GWGCWC 1 cut(s) 1018
BbvCI CCTCAGC 1 cut(s) 822
BbvI GCAGC 5 cut(s) 124, 336, 493, 946, 968
BccI CCATC 2 cut(s) 545, 680
BceAI ACGGC 3 cut(s) 262, 350, 763
BcgI CGANNNNNNTGC 2 cut(s) 638, 672
BcnI CCSGG 2 cut(s) 486, 570
BfaI CTAG 5 cut(s) 666, 759, 834, 843, 1239
BfmI CTRYAG 1 cut(s) 945
BfoI RGCGCY 1 cut(s) 170
BglII AGATCT 1 cut(s) 949
Bme1390I CCNGG 2 cut(s) 486, 570
BmiI GGNNCC 6 cut(s) 168, 250, 650, 840, 922, 1082
BmrFI CCNGG 2 cut(s) 486, 570
BmrI ACTGGG 1 cut(s) 703
BmtI GCTAGC 1 cut(s) 669
BmuI ACTGGG 1 cut(s) 703
Bpu10I CCTNAGC 1 cut(s) 822
Bpu14I TTCGAA 1 cut(s) 356
BpuEI CTTGAG 1 cut(s) 628
BpuMI CCSGG 2 cut(s) 486, 570
Bsa29I ATCGAT 2 cut(s) 50, 1234
BsaHI GRCGYC 2 cut(s) 167, 218
BsaJI CCNNGG 6 cut(s) 177, 252, 332, 485, 569, 879
BsaXI ACNNNNNCTCC 2 cut(s) 442, 472
Bsc4I CCNNNNNNNGG 3 cut(s) 491, 575, 863
Bse118I RCCGGY 1 cut(s) 517
Bse1I ACTGG 3 cut(s) 371, 709, 860
Bse3DI GCAATG 1 cut(s) 904
BseCI ATCGAT 2 cut(s) 50, 1234
BseDI CCNNGG 6 cut(s) 177, 252, 332, 485, 569, 879
BseGI GGATG 1 cut(s) 625
BseLI CCNNNNNNNGG 3 cut(s) 491, 575, 863
BseMI GCAATG 1 cut(s) 904
BseMII CTCAG 1 cut(s) 836
BseNI ACTGG 3 cut(s) 371, 709, 860
BseRI GAGGAG 2 cut(s) 287, 518
BseSI GKGCMC 1 cut(s) 1018
BseXI GCAGC 5 cut(s) 124, 336, 493, 946, 968
BseYI CCCAGC 1 cut(s) 378
BsgI GTGCAG 1 cut(s) 500
Bsh1285I CGRYCG 1 cut(s) 50
BshNI GGYRCC 4 cut(s) 166, 248, 648, 920
BshVI ATCGAT 2 cut(s) 50, 1234
BsiEI CGRYCG 1 cut(s) 50
BsiHKAI GWGCWC 1 cut(s) 1018
BsiSI CCGG 4 cut(s) 485, 518, 569, 670
BslFI GGGAC 1 cut(s) 591
BslI CCNNNNNNNGG 3 cut(s) 491, 575, 863
BsmFI GGGAC 1 cut(s) 591
BsmI GAATGC 2 cut(s) 679, 1138
Bsp119I TTCGAA 1 cut(s) 356
Bsp1286I GDGCHC 2 cut(s) 841, 1018
Bsp143I GATC 6 cut(s) 47, 286, 949, 1204, 1231, 1235
Bsp19I CCATGG 1 cut(s) 879
BspCNI CTCAG 1 cut(s) 835
BspDI ATCGAT 2 cut(s) 50, 1234
BspLI GGNNCC 6 cut(s) 168, 250, 650, 840, 922, 1082
BspMAI CTGCAG 1 cut(s) 949
BspOI GCTAGC 1 cut(s) 669
BspPI GGATC 2 cut(s) 281, 1212
BspT104I TTCGAA 1 cut(s) 356
BspT107I GGYRCC 4 cut(s) 166, 248, 648, 920
BsrBI CCGCTC 1 cut(s) 130
BsrDI GCAATG 1 cut(s) 904
BsrFI RCCGGY 1 cut(s) 517
BsrI ACTGG 3 cut(s) 371, 709, 860
BssAI RCCGGY 1 cut(s) 517
BssECI CCNNGG 6 cut(s) 177, 252, 332, 485, 569, 879
BssMI GATC 6 cut(s) 47, 286, 949, 1204, 1231, 1235
BssNI GRCGYC 2 cut(s) 167, 218
BssT1I CCWWGG 2 cut(s) 252, 879
Bst4CI ACNGT 1 cut(s) 178
Bst6I CTCTTC 3 cut(s) 264, 590, 1177
BstACI GRCGYC 2 cut(s) 167, 218
BstBI TTCGAA 1 cut(s) 356
BstC8I GCNNGC 3 cut(s) 519, 667, 1018
BstDEI CTNAG 1 cut(s) 822
BstDSI CCRYGG 3 cut(s) 177, 332, 879
BstEII GGTNACC 1 cut(s) 4
BstF5I GGATG 1 cut(s) 625
BstH2I RGCGCY 1 cut(s) 170
BstHHI GCGC 2 cut(s) 169, 226
BstKTI GATC 6 cut(s) 50, 289, 952, 1207, 1234, 1238
BstMBI GATC 6 cut(s) 47, 286, 949, 1204, 1231, 1235
BstMCI CGRYCG 1 cut(s) 50
BstMWI GCNNNNNNNGC 5 cut(s) 290, 343, 349, 788, 953
BstNSI RCATGY 1 cut(s) 1069
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 2 cut(s) 484, 568
BstSFI CTRYAG 1 cut(s) 945
BstSLI GKGCMC 1 cut(s) 1018
BstV1I GCAGC 5 cut(s) 124, 336, 493, 946, 968
BstX2I RGATCY 2 cut(s) 949, 1204
BstYI RGATCY 2 cut(s) 949, 1204
Bsu15I ATCGAT 2 cut(s) 50, 1234
BsuTUI ATCGAT 2 cut(s) 50, 1234
BtgI CCRYGG 3 cut(s) 177, 332, 879
BtsCI GGATG 1 cut(s) 625
Cac8I GCNNGC 3 cut(s) 519, 667, 1018
CfoI GCGC 2 cut(s) 169, 226
Cfr10I RCCGGY 1 cut(s) 517
ClaI ATCGAT 2 cut(s) 50, 1234
CseI GACGC 1 cut(s) 207
Csp6I GTAC 1 cut(s) 1102
CviAII CATG 4 cut(s) 116, 880, 1034, 1066
CviQI GTAC 1 cut(s) 1102
DdeI CTNAG 1 cut(s) 822
DinI GGCGCC 1 cut(s) 168
DpnI GATC 6 cut(s) 49, 288, 951, 1206, 1233, 1237
DpnII GATC 6 cut(s) 47, 286, 949, 1204, 1231, 1235
Eam1104I CTCTTC 3 cut(s) 264, 590, 1177
EarI CTCTTC 3 cut(s) 264, 590, 1177
EciI GGCGGA 2 cut(s) 330, 408
Eco130I CCWWGG 2 cut(s) 252, 879
Eco24I GRGCYC 1 cut(s) 841
Eco57I CTGAAG 2 cut(s) 288, 684
Eco91I GGTNACC 1 cut(s) 4
EcoO65I GGTNACC 1 cut(s) 4
EcoRI GAATTC 1 cut(s) 852
EcoT14I CCWWGG 2 cut(s) 252, 879
EcoT38I GRGCYC 1 cut(s) 841
EgeI GGCGCC 1 cut(s) 168
EheI GGCGCC 1 cut(s) 168
ErhI CCWWGG 2 cut(s) 252, 879
FaeI CATG 4 cut(s) 119, 883, 1037, 1069
FalI AAGNNNNNCTT 6 cut(s) 261, 293, 453, 485, 637, 669
FaqI GGGAC 1 cut(s) 591
FatI CATG 4 cut(s) 115, 879, 1033, 1065
FauI CCCGC 1 cut(s) 123
FauNDI CATATG 1 cut(s) 772
FokI GGATG 1 cut(s) 632
FriOI GRGCYC 1 cut(s) 841
FspBI CTAG 5 cut(s) 666, 759, 834, 843, 1239
GlaI GCGC 2 cut(s) 168, 225
GsaI CCCAGC 1 cut(s) 382
HaeII RGCGCY 1 cut(s) 170
HapII CCGG 4 cut(s) 485, 518, 569, 670
HgaI GACGC 1 cut(s) 207
HhaI GCGC 2 cut(s) 169, 226
Hin1I GRCGYC 2 cut(s) 167, 218
Hin1II CATG 4 cut(s) 119, 883, 1037, 1069
Hin6I GCGC 2 cut(s) 167, 224
HinP1I GCGC 2 cut(s) 167, 224
HincII GTYRAC 1 cut(s) 984
HindII GTYRAC 1 cut(s) 984
HindIII AAGCTT 1 cut(s) 696
HinfI GANTC 4 cut(s) 94, 941, 1009, 1118
HpaII CCGG 4 cut(s) 485, 518, 569, 670
HphI GGTGA 5 cut(s) 77, 470, 560, 1040, 1085
Hpy166II GTNNAC 3 cut(s) 101, 984, 1016
Hpy188I TCNGA 8 cut(s) 34, 203, 703, 744, 1117, 1173, 1209, 1231
Hpy188III TCNNGA 4 cut(s) 98, 238, 617, 801
Hpy8I GTNNAC 3 cut(s) 101, 984, 1016
HpyAV CCTTC 9 cut(s) 130, 176, 352, 455, 493, 520, 663, 861, 1227
HpyCH4III ACNGT 1 cut(s) 178
HpyF10VI GCNNNNNNNGC 5 cut(s) 290, 343, 349, 788, 953
HpyF3I CTNAG 1 cut(s) 822
Hsp92I GRCGYC 2 cut(s) 167, 218
Hsp92II CATG 4 cut(s) 119, 883, 1037, 1069
HspAI GCGC 2 cut(s) 167, 224
KasI GGCGCC 1 cut(s) 166
KroI GCCGGC 1 cut(s) 517
KroNI GCCGGC 1 cut(s) 519
Kzo9I GATC 6 cut(s) 47, 286, 949, 1204, 1231, 1235
LmnI GCTCC 3 cut(s) 139, 274, 844
Lsp1109I GCAGC 5 cut(s) 124, 336, 493, 946, 968
MaeI CTAG 5 cut(s) 666, 759, 834, 843, 1239
MaeIII GTNAC 2 cut(s) 4, 465
MalI GATC 6 cut(s) 49, 288, 951, 1206, 1233, 1237
MbiI CCGCTC 1 cut(s) 130
MboI GATC 6 cut(s) 47, 286, 949, 1204, 1231, 1235
MboII GAAGA 6 cut(s) 89, 281, 607, 1018, 1146, 1194
MflI RGATCY 2 cut(s) 949, 1204
MhlI GDGCHC 2 cut(s) 841, 1018
MluCI AATT 7 cut(s) 301, 852, 891, 1038, 1096, 1126, 1162
Mly113I GGCGCC 1 cut(s) 167
MlyI GAGTC 2 cut(s) 103, 950
MmeI TCCRAC 3 cut(s) 181, 406, 844
MroNI GCCGGC 1 cut(s) 517
MroXI GAANNNNTTC 1 cut(s) 871
MseI TTAA 2 cut(s) 56, 1089
MslI CAYNNNNRTG 1 cut(s) 771
MspA1I CMGCKG 4 cut(s) 349, 382, 737, 959
MspI CCGG 4 cut(s) 485, 518, 569, 670
MspR9I CCNGG 2 cut(s) 486, 570
Mva1269I GAATGC 2 cut(s) 679, 1138
MwoI GCNNNNNNNGC 5 cut(s) 290, 343, 349, 788, 953
NaeI GCCGGC 1 cut(s) 519
NarI GGCGCC 1 cut(s) 167
NciI CCSGG 2 cut(s) 486, 570
NcoI CCATGG 1 cut(s) 879
NdeI CATATG 1 cut(s) 772
NdeII GATC 6 cut(s) 47, 286, 949, 1204, 1231, 1235
NgoMIV GCCGGC 1 cut(s) 517
NheI GCTAGC 1 cut(s) 665
NlaIII CATG 4 cut(s) 119, 883, 1037, 1069
NlaIV GGNNCC 6 cut(s) 168, 250, 650, 840, 922, 1082
NmuCI GTSAC 2 cut(s) 4, 465
NspI RCATGY 1 cut(s) 1069
NspV TTCGAA 1 cut(s) 356
PcsI WCGNNNNNNNCGW 1 cut(s) 236
PctI GAATGC 2 cut(s) 679, 1138
PdiI GCCGGC 1 cut(s) 519
PdmI GAANNNNTTC 1 cut(s) 871
PfeI GAWTC 2 cut(s) 1009, 1118
PflMI CCANNNNNTGG 1 cut(s) 863
Ple19I CGATCG 1 cut(s) 50
PleI GAGTC 2 cut(s) 102, 949
PluTI GGCGCC 1 cut(s) 170
PpsI GAGTC 2 cut(s) 102, 949
PspEI GGTNACC 1 cut(s) 4
PspFI CCCAGC 1 cut(s) 378
PspN4I GGNNCC 6 cut(s) 168, 250, 650, 840, 922, 1082
PstI CTGCAG 1 cut(s) 949
PsuI RGATCY 2 cut(s) 949, 1204
PvuI CGATCG 1 cut(s) 50
PvuII CAGCTG 2 cut(s) 737, 959
RsaI GTAC 1 cut(s) 1103
RsaNI GTAC 1 cut(s) 1102
RseI CAYNNNNRTG 1 cut(s) 771
SaqAI TTAA 2 cut(s) 56, 1089
Sau3AI GATC 6 cut(s) 47, 286, 949, 1204, 1231, 1235
SchI GAGTC 2 cut(s) 103, 950
ScrFI CCNGG 2 cut(s) 486, 570
SduI GDGCHC 2 cut(s) 841, 1018
SfcI CTRYAG 1 cut(s) 945
SfoI GGCGCC 1 cut(s) 168
SfuI TTCGAA 1 cut(s) 356
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 1 cut(s) 643
SmoI CTYRAG 1 cut(s) 643
Sse9I AATT 7 cut(s) 301, 852, 891, 1038, 1096, 1126, 1162
SspDI GGCGCC 1 cut(s) 166
SspMI CTAG 5 cut(s) 666, 759, 834, 843, 1239
StyD4I CCNGG 2 cut(s) 484, 568
StyI CCWWGG 2 cut(s) 252, 879
TaaI ACNGT 1 cut(s) 178
TaqI TCGA 5 cut(s) 50, 356, 800, 850, 1234
TasI AATT 7 cut(s) 301, 852, 891, 1038, 1096, 1126, 1162
TauI GCSGC 5 cut(s) 112, 172, 346, 349, 536
TfiI GAWTC 2 cut(s) 1009, 1118
Tru1I TTAA 2 cut(s) 56, 1089
Tru9I TTAA 2 cut(s) 56, 1089
TseFI GTSAC 2 cut(s) 4, 465
TseI GCWGC 5 cut(s) 112, 349, 481, 956, 959
Tsp45I GTSAC 2 cut(s) 4, 465
TspDTI ATGAA 6 cut(s) 132, 744, 783, 989, 1050, 1121
Van91I CCANNNNNTGG 1 cut(s) 863
VneI GTGCAC 1 cut(s) 1014
XapI RAATTY 3 cut(s) 852, 1096, 1126
XceI RCATGY 1 cut(s) 1069
XmnI GAANNNNTTC 1 cut(s) 871
XspI CTAG 5 cut(s) 666, 759, 834, 843, 1239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.