Rroxscaffold_4G00288920

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002776
Physical Location & Seq
Reverse (-)
9569507 .. 9576707
7201 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00288920.1

Sequence Viewer

Length: 3090 bp
ATGGTTACTGATACGTATTCGAAGATGATGTCCGAGATGTCAATGCGATCCATGCTTAAGAACAACGGCGATTACAGTGAAGATTTGAGCCTTCTGATTCGTCAGCAGAGGCAAGAAGTGAGTGAGCGAGAGAAGGAGCTCAATCCATACAGAAGTGGGTCGGCCCCACCAACCGTAGAAGGGTCGCTGAACGCCGTCGGCGGTCTTTTCGACGCCGTTAACGGCTTTGCGAGCGAGGAGGAGCTAAGGGCTGATCCGGCTTATCATAGCTACTACTACGCCAATGTCAATCTCAATCCGCGGCTACCGCCGCCGCTGAGGTCCAAGGAGGACTGGCGGTTTGCGCAGCGGGGCAGCGCCGGAGGAGGTTCTGGGGTCGGAGGCATTGGGGATAGGAGGAAAGGCGGGAGGGGTGGTGGTGGTGAAGGGTCGCTTTTTTCGGTGCAGCCGGAGAATGGGGCGGCGGCGAGGAATGGAGAGTGGGGCGGAGATGGGCTGATTGGGTTGCCCGGGTTGGGCCTCGGGAGTAGGCAGAAGAGTATTGCCGAGATTCTTCAGGATGATATACAGAATACAACTGGCTCAAGGCACCCTTCTCGTCCAGCTAGCCGGAATGCATTTGATGATGGTGTTGAAACTTCAGATACCCAGTATGCTCAGATGCATCGTGATTTGGCATCTTTAGATGCTTTGCGCTCTGGTGGAAATAAGCAGGGTATGTCTGCTGCCCAGAACTTTGGTTCCTCGGGCTCTCATACTTATGCTTCTGCTCTAGGTGGTTCACTGTCAAGAAGCACCACACCAGATCCTCAGCTTGTATCTAGGGCTCCTAGTCCCCGGATTCCACCAGTTGGAGGAGGAAGAGCTCCTTCCACTGATAAAAATAGTGTCGGTGGTCAAAATTCATATAATGGTATCACATCCAATGTGAAAGAGTCTGCAGATCTGGTAGCTGCCTTATCTGGTATGAACCTGTCAAAAAACAGCAGGATGCATGAAGAGAATCATGCACATTCACAGATTCAAGGTGATAACCACTTTGATATGCAAGGGGATCGGAACCATATTAAACAAAATTCATATATGAACAAGCCTGATTCAGGGAATTATCATTTGCATTCTGTTTCCCAGTCTACCAAAAGCTCTTATCAGAATATGGCCAAAGGCAGTGGATTTGGGAGGGACCAGAACAACCCTTCGCTGATGTCAGATGGGCTAGCTGAAATTAACAATCCTGCTATATCTTCTGCTAACTCGTACTTGAGAGGACCCTCTGCCCCAGCTCTTAATGGCCGAGGAAGCTCAGTCTCTCACTATCAGAATGTAGATGGTATGAATTCCTCATTTGCAAACTATGGGTTAGCTGGGTATTCTGTTAGTCCATCGTCACCCTCCATGATGGGGAGCCCACTGGGTAATGGTAATTTACCACCCTTGTTTGAAAATGCTGCTGCTGTTTCTGCAATGGGTGGCTTGGACTCTGGAGCCTTTGGAGGAGGTATGTCTCTGGGACCAAATTTGTTAGCAGCAGCTGCTGAATTGCAGAATATGGGGAGGGGCGGAAATCACACTGCCGGAGGTGCTCTTCAGATGCCCTTGATGGACCCCTTGTATCTGCAGTATTTAAGATCAAATGAGTATGCTGCTGCACAGCTTGCATCCCTTCATGACCCCACAGCAGACAGGGAAGGCATGTATATGGACTTTCTGGGGTTGCAAAAAGCTTATCTGGGACAATTACTTTCGCCTCAAAAATCACAGTTTGGTGCTCCCTACATGGGTAAGTCTGGTAGCTTGAATCATGGTTATTATGGAAATCCAGCATTTGGGCTTGGTATGTCTTATTCTGGGAGTCCCCTTCTTCCAAACTCTCCCGTTGGACCAGGTAGTCCTGTCAGGCACAGTGACAGGAACTTGCGTTTTTCTTCGGGGATGAGGAACATGAGTGGAGGTCTCATGGGAGCTTGGCACTCTGAAACTGGTGGAAATTTCGATGAAAGTTTTGCATCCTCTTTGCTAGATGAGTTCAAGAGCAATAAGACTAAATGTTTTGAACTTTCAGAAATTTCGGGCCATGTTGTTGAGTTCAGTGCGGACCAGTATGGGAGTAGGTTCATTCAACAGAAACTTGAAACTGCCACAACCGAAGAAAAGAACATGGTTTTTGATGAAATTATGCCCCAAGCCCTTTCTTTGATGACTGATGTGTTTGGTAATTATGTGATTCAGAAGTTTTTCGAGCACGGTTCTGCAGCACAAATTAGAGAACTGGCTGACCAGCTCACTGGGCATGTGCTGACCCTTAGCCTTCAAATGTATGGCTGTCGAGTGATCCAGAAGGCTATAGAAGTTGTTGATCTGGACCAGCAGACTCAAATGGTCACAGAACTGGATGGTCACATTATGCGTTGTGTTCGTGATCAAAATGGGAACCATGTCATCCAGAAGTGTATTGAATGTATACCTGAAGATGCTATCCAGTTTGTTGTTTCAACCTTTTATGATCAAGTTGTGACACTGTCAACTCATCCATATGGTTGTCGTGTCATACAGAGAATTTTGGAGCACTGCCATGATCCCAAAACGCAGCAGATAATGATGGATGAGATTTTGCATGCTGTTTGCACTTTGGCACAAGATCAGTATGGAAATTATGTTGTGCAGCATGTGCTGGAGCATGGAATGCCAGACGAGCGCTCAGATATAATTAGGAAACTAACTGGGCAGATTGTTCAAATGAGCCAGCAGAAATTTGCCTCCAATGTTATAGAAAAGTGTTTAACTTTTGGAACTCTTGCTGAGCGCCAGGCCCTTGTAACTGAGATGCTCGGTACCACTGATGAAAACGAACCCCTTCAGGCAATGATGAAGGACCAGTTTGCAAATTATGTTGTACAGAAAGTTTTGGAAACTTGTGATGACCAGCAGCTTGAACTAATCCTTAACCGTATTAAGGTTCATCTGAATGCTCTGAAGAAGTATACTTATGGGAAGCATATAGTTGCACGTGTAGAGAAACTTGTTGCTGCTGGAGGTACTAGGATTAGCATCCTGACTCCACAATGTCCACCTGCTACTGCTACTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1029

Amino Acids

111.17

Weight (kDa)

5.89

Isoelectric Point (pI)

49.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 275 - 687 2.9e-150 Nucleic acid binding protein NABP
PUF PF00806 692 - 726 8e-12 Pumilio-family RNA binding repeat
PUF PF00806 729 - 761 4.7e-08 Pumilio-family RNA binding repeat
PUF PF00806 764 - 797 1.9e-09 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 794 - 879 1e-05 NOP9-like PUF repeat domain
PUF PF00806 836 - 871 2.4e-13 Pumilio-family RNA binding repeat
PUF PF00806 874 - 906 6.7e-09 Pumilio-family RNA binding repeat
PUF PF00806 909 - 942 2.1e-07 Pumilio-family RNA binding repeat
PUF PF00806 955 - 984 9.4e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3079
AasI GACNNNNNNGTC 1 cut(s) 1887
Acc16I TGCGCA 1 cut(s) 345
Acc36I ACCTGC 1 cut(s) 3079
Acc65I GGTACC 1 cut(s) 2831
AccB1I GGYRCC 2 cut(s) 588, 2831
AccB7I CCANNNNNTGG 2 cut(s) 851, 1826
AccI GTMKAC 3 cut(s) 1133, 2461, 2981
AccII CGCG 1 cut(s) 301
AclWI GGATC 6 cut(s) 42, 248, 800, 1062, 2326, 2570
AcoI YGGCCR 2 cut(s) 1158, 1291
AcsI RAATTY 8 cut(s) 901, 1075, 1336, 1516, 1987, 2064, 2556, 2750
AcuI CTGAAG 6 cut(s) 539, 624, 1571, 2487, 2840, 2993
AcvI CACGTG 1 cut(s) 3008
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 4 cut(s) 1259, 2833, 2895, 3037
AfeI AGCGCT 1 cut(s) 2696
AflII CTTAAG 1 cut(s) 56
AflIII ACRYGT 1 cut(s) 3007
AjnI CCWGG 2 cut(s) 1882, 2805
AloI GAACNNNNNNTCC 2 cut(s) 725, 757
Alw21I GWGCWC 6 cut(s) 141, 868, 1585, 1771, 2244, 2568
Alw26I GTCTC 3 cut(s) 1312, 1509, 1958
AlwI GGATC 6 cut(s) 42, 248, 800, 1062, 2326, 2570
AlwNI CAGNNNCTG 2 cut(s) 1532, 1535
Ama87I CYCGRG 3 cut(s) 509, 521, 745
Aor51HI AGCGCT 1 cut(s) 2696
AoxI GGCC 6 cut(s) 162, 517, 1158, 1291, 2071, 2808
ApoI RAATTY 8 cut(s) 901, 1075, 1336, 1516, 1987, 2064, 2556, 2750
ArsI GACNNNNNNTTYG 4 cut(s) 14, 46, 323, 355
Asp700I GAANNNNTTC 2 cut(s) 2234, 2853
Asp718I GGTACC 1 cut(s) 2831
AspLEI GCGC 5 cut(s) 346, 359, 696, 2697, 2805
AsuC2I CCSGG 3 cut(s) 510, 511, 838
AsuHPI GGTGA 3 cut(s) 434, 1040, 1380
AsuII TTCGAA 1 cut(s) 20
AsuNHI GCTAGC 2 cut(s) 605, 1216
AvaI CYCGRG 3 cut(s) 509, 521, 745
AvaII GGWCC 9 cut(s) 321, 1183, 1268, 1511, 1603, 1880, 2095, 2362, 2872
BalI TGGCCA 1 cut(s) 1160
BanI GGYRCC 2 cut(s) 588, 2831
BanII GRGCYC 5 cut(s) 141, 752, 829, 868, 1409
BbrPI CACGTG 1 cut(s) 3008
Bbv12I GWGCWC 6 cut(s) 141, 868, 1585, 1771, 2244, 2568
BbvCI CCTCAGC 2 cut(s) 317, 810
BccI CCATC 9 cut(s) 485, 620, 1205, 1322, 1390, 1393, 1594, 2387, 2593
BceAI ACGGC 4 cut(s) 82, 179, 200, 238
BcgI CGANNNNNNTGC 4 cut(s) 578, 612, 1037, 1071
BciT130I CCWGG 2 cut(s) 1884, 2807
BclI TGATCA 2 cut(s) 2419, 2503
BcnI CCSGG 3 cut(s) 510, 511, 838
BcoDI GTCTC 3 cut(s) 1312, 1509, 1958
BfaI CTAG 7 cut(s) 606, 773, 822, 831, 1217, 2018, 3039
BfmI CTRYAG 4 cut(s) 939, 1616, 2250, 2343
BfoI RGCGCY 3 cut(s) 360, 2698, 2806
BfrI CTTAAG 1 cut(s) 56
BfuAI ACCTGC 1 cut(s) 3079
BglII AGATCT 1 cut(s) 943
BlpI GCTNAGC 1 cut(s) 2799
Bme1390I CCNGG 5 cut(s) 510, 511, 838, 1884, 2807
Bme18I GGWCC 9 cut(s) 321, 1183, 1268, 1511, 1603, 1880, 2095, 2362, 2872
BmeT110I CYCGRG 3 cut(s) 509, 521, 745
BmrFI CCNGG 5 cut(s) 510, 511, 838, 1884, 2807
BmrI ACTGGG 5 cut(s) 643, 1123, 1421, 2295, 2730
BmtI GCTAGC 2 cut(s) 609, 1220
BmuI ACTGGG 5 cut(s) 643, 1123, 1421, 2295, 2730
BpmI CTGGAG 3 cut(s) 1503, 2693, 3051
Bpu10I CCTNAGC 4 cut(s) 245, 317, 810, 2303
Bpu1102I GCTNAGC 1 cut(s) 2799
Bpu14I TTCGAA 1 cut(s) 20
BpuEI CTTGAG 2 cut(s) 568, 1282
BpuMI CCSGG 3 cut(s) 510, 511, 838
BsaAI YACGTR 2 cut(s) 15, 3008
BsaBI GATNNNNATC 1 cut(s) 3047
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 1958
BsaJI CCNNGG 7 cut(s) 299, 324, 509, 520, 744, 836, 1294
BsaXI ACNNNNNCTCC 2 cut(s) 400, 430
Bse3DI GCAATG 2 cut(s) 1470, 2868
Bse8I GATNNNNATC 1 cut(s) 3047
BseBI CCWGG 2 cut(s) 1884, 2807
BseDI CCNNGG 7 cut(s) 299, 324, 509, 520, 744, 836, 1294
BseJI GATNNNNATC 1 cut(s) 3047
BseMI GCAATG 2 cut(s) 1470, 2868
BseMII CTCAG 7 cut(s) 308, 671, 824, 1317, 2712, 2790, 2811
BseRI GAGGAG 5 cut(s) 251, 254, 378, 870, 1509
BseYI CCCAGC 2 cut(s) 1279, 1364
BsgI GTGCAG 3 cut(s) 464, 1632, 2681
Bsh1236I CGCG 1 cut(s) 301
BshFI GGCC 6 cut(s) 164, 519, 1160, 1293, 2073, 2810
BshNI GGYRCC 2 cut(s) 588, 2831
BsiHKAI GWGCWC 6 cut(s) 141, 868, 1585, 1771, 2244, 2568
BsiHKCI CYCGRG 3 cut(s) 509, 521, 745
BsiSI CCGG 7 cut(s) 257, 360, 449, 510, 610, 838, 1575
BslFI GGGAC 5 cut(s) 819, 1196, 1524, 1746, 1839
BsmAI GTCTC 3 cut(s) 1312, 1509, 1958
BsmFI GGGAC 5 cut(s) 819, 1196, 1524, 1746, 1839
BsmI GAATGC 4 cut(s) 619, 1117, 2688, 2971
BsnI GGCC 6 cut(s) 164, 519, 1160, 1293, 2073, 2810
Bso31I GGTCTC 1 cut(s) 1958
BsoBI CYCGRG 3 cut(s) 509, 521, 745
Bsp119I TTCGAA 1 cut(s) 20
Bsp1286I GDGCHC 9 cut(s) 141, 752, 829, 868, 1409, 1585, 1771, 2244, 2568
Bsp1407I TGTACA 1 cut(s) 2893
Bsp1720I GCTNAGC 1 cut(s) 2799
BspANI GGCC 6 cut(s) 164, 519, 1160, 1293, 2073, 2810
BspCNI CTCAG 7 cut(s) 309, 670, 823, 1316, 2711, 2791, 2812
BspFNI CGCG 1 cut(s) 301
BspHI TCATGA 1 cut(s) 1666
BspMAI CTGCAG 3 cut(s) 943, 1620, 2254
BspMI ACCTGC 1 cut(s) 3079
BspOI GCTAGC 2 cut(s) 609, 1220
BspPI GGATC 6 cut(s) 42, 248, 800, 1062, 2326, 2570
BspQI GCTCTTC 2 cut(s) 856, 1590
BspT104I TTCGAA 1 cut(s) 20
BspT107I GGYRCC 2 cut(s) 588, 2831
BspTI CTTAAG 1 cut(s) 56
BspTNI GGTCTC 1 cut(s) 1958
BsrDI GCAATG 2 cut(s) 1470, 2868
BsrGI TGTACA 1 cut(s) 2893
BssECI CCNNGG 7 cut(s) 299, 324, 509, 520, 744, 836, 1294
BssNAI GTATAC 2 cut(s) 2462, 2982
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 1 cut(s) 324
Bst1107I GTATAC 2 cut(s) 2462, 2982
Bst2UI CCWGG 2 cut(s) 1884, 2807
Bst4CI ACNGT 8 cut(s) 77, 175, 786, 1761, 1904, 2246, 2520, 2948
Bst6I CTCTTC 4 cut(s) 530, 856, 993, 1590
BstACI GRCGYC 1 cut(s) 213
BstAFI CTTAAG 1 cut(s) 56
BstAPI GCANNNNNTGC 4 cut(s) 1532, 1655, 2668, 2683
BstAUI TGTACA 1 cut(s) 2893
BstBAI YACGTR 2 cut(s) 15, 3008
BstBI TTCGAA 1 cut(s) 20
BstC8I GCNNGC 6 cut(s) 232, 607, 1218, 1656, 2616, 2744
BstDSI CCRYGG 1 cut(s) 299
BstENI CCTNNNNNAGG 1 cut(s) 1098
BstFNI CGCG 1 cut(s) 301
BstH2I RGCGCY 3 cut(s) 360, 2698, 2806
BstHHI GCGC 5 cut(s) 346, 359, 696, 2697, 2805
BstMAI GTCTC 3 cut(s) 1312, 1509, 1958
BstNI CCWGG 2 cut(s) 1884, 2807
BstNSI RCATGY 4 cut(s) 1696, 2294, 2618, 2669
BstSCI CCNGG 5 cut(s) 508, 509, 836, 1882, 2805
BstSFI CTRYAG 4 cut(s) 939, 1616, 2250, 2343
BstSNI TACGTA 1 cut(s) 15
BstUI CGCG 1 cut(s) 301
BstX2I RGATCY 2 cut(s) 805, 943
BstXI CCANNNNNNTGG 2 cut(s) 737, 2285
BstYI RGATCY 2 cut(s) 805, 943
BstZ17I GTATAC 2 cut(s) 2462, 2982
BsuRI GGCC 6 cut(s) 164, 519, 1160, 1293, 2073, 2810
BtgI CCRYGG 1 cut(s) 299
BtsI GCAGTG 3 cut(s) 1174, 1569, 2566
BveI ACCTGC 1 cut(s) 3079
Cac8I GCNNGC 6 cut(s) 232, 607, 1218, 1656, 2616, 2744
CaiI CAGNNNCTG 2 cut(s) 1532, 1535
CciI TCATGA 1 cut(s) 1666
CfoI GCGC 5 cut(s) 346, 359, 696, 2697, 2805
Cfr42I CCGCGG 1 cut(s) 302
Cfr9I CCCGGG 1 cut(s) 509
CseI GACGC 1 cut(s) 221
CsiI ACCWGGT 1 cut(s) 1882
Csp6I GTAC 4 cut(s) 1258, 2832, 2894, 3036
CspCI CAANNNNNGTGG 2 cut(s) 1150, 1185
CviQI GTAC 4 cut(s) 1258, 2832, 2894, 3036
DrdI GACNNNNNNGTC 1 cut(s) 1887
DseDI GACNNNNNNGTC 1 cut(s) 1887
EaeI YGGCCR 2 cut(s) 1158, 1291
Eam1104I CTCTTC 4 cut(s) 530, 856, 993, 1590
EarI CTCTTC 4 cut(s) 530, 856, 993, 1590
EciI GGCGGA 2 cut(s) 501, 1575
Ecl136II GAGCTC 2 cut(s) 139, 866
Eco105I TACGTA 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 324
Eco24I GRGCYC 5 cut(s) 141, 752, 829, 868, 1409
Eco31I GGTCTC 1 cut(s) 1958
Eco47I GGWCC 9 cut(s) 321, 1183, 1268, 1511, 1603, 1880, 2095, 2362, 2872
Eco47III AGCGCT 1 cut(s) 2696
Eco53kI GAGCTC 2 cut(s) 139, 866
Eco57I CTGAAG 6 cut(s) 539, 624, 1571, 2487, 2840, 2993
Eco72I CACGTG 1 cut(s) 3008
Eco88I CYCGRG 3 cut(s) 509, 521, 745
EcoICRI GAGCTC 2 cut(s) 139, 866
EcoNI CCTNNNNNAGG 1 cut(s) 1098
EcoO109I RGGNCCY 2 cut(s) 1268, 2809
EcoRI GAATTC 1 cut(s) 1336
EcoRII CCWGG 2 cut(s) 1882, 2805
EcoT14I CCWWGG 1 cut(s) 324
EcoT22I ATGCAT 3 cut(s) 619, 666, 996
EcoT38I GRGCYC 5 cut(s) 141, 752, 829, 868, 1409
ErhI CCWWGG 1 cut(s) 324
FalI AAGNNNNNCTT 6 cut(s) 417, 449, 577, 609, 853, 885
FaqI GGGAC 5 cut(s) 819, 1196, 1524, 1746, 1839
FauI CCCGC 2 cut(s) 342, 398
FauNDI CATATG 1 cut(s) 2533
FbaI TGATCA 2 cut(s) 2419, 2503
FblI GTMKAC 3 cut(s) 1133, 2461, 2981
FriOI GRGCYC 5 cut(s) 141, 752, 829, 868, 1409
FspBI CTAG 7 cut(s) 606, 773, 822, 831, 1217, 2018, 3039
FspI TGCGCA 1 cut(s) 345
GlaI GCGC 5 cut(s) 345, 358, 695, 2696, 2804
GsaI CCCAGC 2 cut(s) 1283, 1368
GsuI CTGGAG 3 cut(s) 1503, 2693, 3051
HaeII RGCGCY 3 cut(s) 360, 2698, 2806
HaeIII GGCC 6 cut(s) 164, 519, 1160, 1293, 2073, 2810
HapII CCGG 7 cut(s) 257, 360, 449, 510, 610, 838, 1575
HgaI GACGC 1 cut(s) 221
HhaI GCGC 5 cut(s) 346, 359, 696, 2697, 2805
Hin1I GRCGYC 1 cut(s) 213
Hin6I GCGC 5 cut(s) 344, 357, 694, 2695, 2803
HinP1I GCGC 5 cut(s) 344, 357, 694, 2695, 2803
HincII GTYRAC 2 cut(s) 220, 2523
HindII GTYRAC 2 cut(s) 220, 2523
HindIII AAGCTT 1 cut(s) 1722
HpaI GTTAAC 1 cut(s) 220
HpaII CCGG 7 cut(s) 257, 360, 449, 510, 610, 838, 1575
HphI GGTGA 3 cut(s) 434, 1040, 1380
Hpy166II GTNNAC 7 cut(s) 220, 782, 1134, 2462, 2523, 2982, 3068
Hpy8I GTNNAC 7 cut(s) 220, 782, 1134, 2462, 2523, 2982, 3068
Hpy99I CGWCG 2 cut(s) 200, 215
HpyCH4III ACNGT 8 cut(s) 77, 175, 786, 1761, 1904, 2246, 2520, 2948
HpyCH4IV ACGT 2 cut(s) 14, 3007
HpySE526I ACGT 2 cut(s) 14, 3007
Hsp92I GRCGYC 1 cut(s) 213
HspAI GCGC 5 cut(s) 344, 357, 694, 2695, 2803
KpnI GGTACC 1 cut(s) 2835
Ksp22I TGATCA 2 cut(s) 2419, 2503
KspAI GTTAAC 1 cut(s) 220
KspI CCGCGG 1 cut(s) 302
LguI GCTCTTC 2 cut(s) 856, 1590
MabI ACCWGGT 1 cut(s) 1882
MaeI CTAG 7 cut(s) 606, 773, 822, 831, 1217, 2018, 3039
MaeII ACGT 2 cut(s) 14, 3007
MaeIII GTNAC 7 cut(s) 4, 1386, 1904, 2380, 2396, 2512, 2815
MflI RGATCY 2 cut(s) 805, 943
MhlI GDGCHC 9 cut(s) 141, 752, 829, 868, 1409, 1585, 1771, 2244, 2568
MlsI TGGCCA 1 cut(s) 1160
MluNI TGGCCA 1 cut(s) 1160
MlyI GAGTC 5 cut(s) 944, 1472, 1861, 2365, 3049
MmeI TCCRAC 3 cut(s) 358, 832, 1858
Mox20I TGGCCA 1 cut(s) 1160
Mph1103I ATGCAT 3 cut(s) 619, 666, 996
MroXI GAANNNNTTC 2 cut(s) 2234, 2853
MscI TGGCCA 1 cut(s) 1160
MseI TTAA 9 cut(s) 57, 219, 1068, 1227, 1287, 1625, 2780, 2943, 2952
MslI CAYNNNNRTG 5 cut(s) 759, 1697, 2532, 2571, 2964
Msp20I TGGCCA 1 cut(s) 1160
MspA1I CMGCKG 4 cut(s) 301, 316, 349, 1532
MspCI CTTAAG 1 cut(s) 56
MspI CCGG 7 cut(s) 257, 360, 449, 510, 610, 838, 1575
MspR9I CCNGG 5 cut(s) 510, 511, 838, 1884, 2807
Mva1269I GAATGC 4 cut(s) 619, 1117, 2688, 2971
MvaI CCWGG 2 cut(s) 1884, 2807
MvnI CGCG 1 cut(s) 301
NciI CCSGG 3 cut(s) 510, 511, 838
NdeI CATATG 1 cut(s) 2533
NheI GCTAGC 2 cut(s) 605, 1216
NmeAIII GCCGAG 2 cut(s) 571, 1319
NmuCI GTSAC 5 cut(s) 1386, 1904, 2380, 2396, 2512
NsbI TGCGCA 1 cut(s) 345
NsiI ATGCAT 3 cut(s) 619, 666, 996
NspI RCATGY 4 cut(s) 1696, 2294, 2618, 2669
NspV TTCGAA 1 cut(s) 20
PaeI GCATGC 1 cut(s) 2618
PagI TCATGA 1 cut(s) 1666
PaqCI CACCTGC 1 cut(s) 3079
PciSI GCTCTTC 2 cut(s) 856, 1590
PctI GAATGC 4 cut(s) 619, 1117, 2688, 2971
PdmI GAANNNNTTC 2 cut(s) 2234, 2853
PfeI GAWTC 8 cut(s) 97, 550, 841, 1003, 1021, 1097, 1798, 2224
PflFI GACNNNGTC 1 cut(s) 2518
PflMI CCANNNNNTGG 2 cut(s) 851, 1826
PleI GAGTC 5 cut(s) 943, 1472, 1860, 2365, 3049
PmaCI CACGTG 1 cut(s) 3008
PmlI CACGTG 1 cut(s) 3008
PpsI GAGTC 5 cut(s) 943, 1472, 1860, 2365, 3049
Ppu21I YACGTR 2 cut(s) 15, 3008
PpuMI RGGWCCY 1 cut(s) 1268
Psp124BI GAGCTC 2 cut(s) 141, 868
Psp5II RGGWCCY 1 cut(s) 1268
Psp6I CCWGG 2 cut(s) 1882, 2805
PspCI CACGTG 1 cut(s) 3008
PspFI CCCAGC 2 cut(s) 1279, 1364
PspGI CCWGG 2 cut(s) 1882, 2805
PspPPI RGGWCCY 1 cut(s) 1268
PstI CTGCAG 3 cut(s) 943, 1620, 2254
PstNI CAGNNNCTG 2 cut(s) 1532, 1535
PsuI RGATCY 2 cut(s) 805, 943
PsyI GACNNNGTC 1 cut(s) 2518
PvuII CAGCTG 1 cut(s) 1532
RsaI GTAC 4 cut(s) 1259, 2833, 2895, 3037
RsaNI GTAC 4 cut(s) 1258, 2832, 2894, 3036
RseI CAYNNNNRTG 5 cut(s) 759, 1697, 2532, 2571, 2964
SacI GAGCTC 2 cut(s) 141, 868
SacII CCGCGG 1 cut(s) 302
SapI GCTCTTC 2 cut(s) 856, 1590
SaqAI TTAA 9 cut(s) 57, 219, 1068, 1227, 1287, 1625, 2780, 2943, 2952
SchI GAGTC 5 cut(s) 944, 1472, 1861, 2365, 3049
ScrFI CCNGG 5 cut(s) 510, 511, 838, 1884, 2807
SduI GDGCHC 9 cut(s) 141, 752, 829, 868, 1409, 1585, 1771, 2244, 2568
SexAI ACCWGGT 1 cut(s) 1882
SfcI CTRYAG 4 cut(s) 939, 1616, 2250, 2343
Sfr303I CCGCGG 1 cut(s) 302
SfuI TTCGAA 1 cut(s) 20
SgrBI CCGCGG 1 cut(s) 302
SinI GGWCC 9 cut(s) 321, 1183, 1268, 1511, 1603, 1880, 2095, 2362, 2872
SmaI CCCGGG 1 cut(s) 511
SmiMI CAYNNNNRTG 5 cut(s) 759, 1697, 2532, 2571, 2964
SmlI CTYRAG 3 cut(s) 56, 583, 1261
SmoI CTYRAG 3 cut(s) 56, 583, 1261
SnaBI TACGTA 1 cut(s) 15
SphI GCATGC 1 cut(s) 2618
SspMI CTAG 7 cut(s) 606, 773, 822, 831, 1217, 2018, 3039
SstI GAGCTC 2 cut(s) 141, 868
StyD4I CCNGG 5 cut(s) 508, 509, 836, 1882, 2805
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 8 cut(s) 77, 175, 786, 1761, 1904, 2246, 2520, 2948
TaiI ACGT 2 cut(s) 17, 3010
TaqI TCGA 5 cut(s) 20, 210, 1992, 2238, 2326
TatI WGTACW 1 cut(s) 2893
TauI GCSGC 5 cut(s) 304, 313, 316, 464, 467
TfiI GAWTC 8 cut(s) 97, 550, 841, 1003, 1021, 1097, 1798, 2224
Tru1I TTAA 9 cut(s) 57, 219, 1068, 1227, 1287, 1625, 2780, 2943, 2952
Tru9I TTAA 9 cut(s) 57, 219, 1068, 1227, 1287, 1625, 2780, 2943, 2952
TseFI GTSAC 5 cut(s) 1386, 1904, 2380, 2396, 2512
Tsp45I GTSAC 5 cut(s) 1386, 1904, 2380, 2396, 2512
TspMI CCCGGG 1 cut(s) 509
Tth111I GACNNNGTC 1 cut(s) 2518
Van91I CCANNNNNTGG 2 cut(s) 851, 1826
Vha464I CTTAAG 1 cut(s) 56
VpaK11BI GGWCC 9 cut(s) 321, 1183, 1268, 1511, 1603, 1880, 2095, 2362, 2872
XagI CCTNNNNNAGG 1 cut(s) 1098
XapI RAATTY 8 cut(s) 901, 1075, 1336, 1516, 1987, 2064, 2556, 2750
XceI RCATGY 4 cut(s) 1696, 2294, 2618, 2669
XmaI CCCGGG 1 cut(s) 509
XmiI GTMKAC 3 cut(s) 1133, 2461, 2981
XmnI GAANNNNTTC 2 cut(s) 2234, 2853
XspI CTAG 7 cut(s) 606, 773, 822, 831, 1217, 2018, 3039
Zsp2I ATGCAT 3 cut(s) 619, 666, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.