FvH4_7g22880

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002776
Physical Location & Seq
Forward (+)
17977228 .. 17984714
7487 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g22880.t1

Sequence Viewer

Length: 2880 bp
ATGGTTACTGATACGTATTCGAAGATGATGTCCGAGATGTCAATGCGATCCATGCTCAAGAACAACGGCGGCGATTACAGTGAAGATTTGAGTCTGTTGATTCGTCAGCAGAGGCAAGAAGTGAGTGACAGAGAGAAGGAGCTCAATCCATATCGAAGTGGGTCGGCCCCACCAACCGTTGAAGGGTCCCTGAACGCCGTCGGCGGCCTTGTAGACGACGTTAACACCGAGGAGGAGCTCCGGTCTGATCCGGCTTATCATAAGTTTTACTATGCAAATGTCAATCTCAATCCGCGGCTGCCGCCGCCGATGAGGTCCAAGGAGGAGTGGCGGTTTGCGCAGCGGGGCGGCGGCGGAGGAGGGTCTGGGGTTGGAGGCATTGGGGATAGGAGGAAAGGCGGGAGGGGTGGTGGTGAAGGTTCGCTTTTTTCGGTGCAGCCGGAGAATGGGGCGGCGGCAAGGAATGGAGAGTGGGGAGGAGATGGGCTGATTGGGTTGCCCGGGTTGGGGCTTGGGAGTAGGCAGAAGAGTATTGCGGAGATTCTTCAGGATGATATACAGAATACAAGTGGCTCAAGGCACCCTTCTCGCCCAGCTAGCCGGAATGCGTTTGATGATGGTGTTGAAACTTCAGACACCCAGTATGCTCAGATGCATCGTGATCTGGCAGCTTTAGATGCTTTGCGCTCTGGTGGAAATAAGCAGGGTTTGTCTGCTGCCCAGAACTTCGGTTCCTCGGGTTCTCATACTTATGCTTCTGCTCTAGGTGGTTCACTGTCAAGAAGCACCACACCAGATCCTCAGCTTGTATCTAGGGCTCCTAGTCCTCGGATTCCAACTGTTGGAGGAGGAAGAGCTTCTTCCACTGATAAAAATAATGTCAGTGGTCAAAATACGTATAATGGCATCACAGCCAATGTGAATGAGTCTGCAGATCTGGTTGCTGCATTATCTGGTATGAACCTGTCAAAGAACGGCAGGATGCATGAAGAGAATCTTGCACATTCACAGATTCAAGGTGATAACCACTTTGATATGCAAGGGGATCGGAACCATATCAAACAAAATTCATATATGAACAAGTCCCATCTGCATTCTGTTTCCCAGTCTACCAAAAACTCTTATCAGAATATGGCCAAAGGTAGTGGATTTGGGAGGGATCAGAACAACCCTTCATTGATGTCTGATGGGCTAGCTGAAATTAATAATCCTGCTGTATCATCTGCTAACTCATACTTGAGAGGACCCTCTTTACCAGCTCTTAATGGCAGAGGAAGCTCAGTCTCTCATTATCAGAATGTAGACAATATGAATTCCTCATATGCGAACTATGGGTTGGCTGGGTATCCTGTTAGTCCATCGTCACCCTCTATGATGGGGAGCCCACTGGGTAATGGTAATTTACCACCCTTGTTTGAAAATGCTGCTGCTGCCTCTGCAATGAGTGGCTTGGACTCTGGAGCTTTTGGAGGAGGCATGTATATGGACTTGCTGGGGCTGCAGAAAGCTTATCTGGGACAATTGCTTTCGCCTCAAAAGTCACAGTTTGGTGCTCCCTACATGGGTAAATCTGGTAGCTTGAATCATGGTTATTATGGAAATCCAGCATTTGGGCTCGGCATGTCTTATTCTGGAAACCCCCTTCTCCCAAACTCTCCCGTGGGACCAGGTAGTCCTGTTAGGCATAGTGACAGGAACATACGTTTTTCTTCGGGGATGAGGAACATGAGTGGAGGTCTCATGGGAGCTTGGCACTCAGAAACTGGTGGAAACTTCGATGATAGTTTTGCATCCTCTTTGCTGGATGAGTTCAAGAGCAATAAGACTAAATGTTTTGAACTTTCAGAAATTTCTGGCCATGTTGTTGAGTTCAGTGCGGACCAGTATGGGAGTCGGTTCATTCAACAGAAGCTTGAAACTGCCACAACCGAAGAAAAGAACATGGTTTTTGATGAAATTATGCCCCAAGCCCTCTCTTTGATGACCGATGTGTTCGGTAATTATGTGATTCAGAAGTTTTTCGAGCATGGTTCTGCAGCACAAATTAGAGAACTCGCTGATCAGCTCACTGGGCATGTGCTGACCCTAAGCCTTCAAATGTATGGCTGTCGAGTGATCCAGAAGGCTATAGAAGTTGTTGATCTGGACCAGCAGACTCAAATGGTCACAGAACTGGATGGACACATTATGCGTTGTGTTCGTGATCAGAATGGGAACCATGTCATCCAGAAGTGTATTGAATGTATACCTGAAGATGCTATCCAGTTTGTTGTTTCAACCTTTTATGATCAGGTTGTGACACTGTCAACTCATCCATATGGTTGTCGTGTCATACAGAGAATTTTGGAGCACTGCCATGATCCCAACACACAGCAGATAATGATGGATGAGATTCTGCATGCTGTTTGCACTTTGGCACAAGATCAGTATGGAAATTATGTTGTACAGCATGTGCTGGAGCATGGAAAGCCAGATGAGCGCTCAGATATAATTAGGAAGCTAACTGGGCAGATTGTTCAAATGAGCCAGCAGAAATTTGCCTCCAATGTTATAGAAAAGTGTTTAACTTTTGGAACTCTTGCTGAGCGCCAGGCCCTTGTCACTGAGATGCTTGGTACCACTGATGAAAACGAACCCCTTCAGGCAATGATGAAGGACCAGTTCGCAAATTATGTTGTACAAAAAGTTTTGGAAACTTGTGATGACCAGCAACTTGAGCTAATCCTTAACCGTATTAAGGTTCATCTGAATGCTCTGAAGAAGTATACTTATGGGAAGCATATAGTTGCACGTGTAGAGAAACTTGTTGCTGCTGGAGAAAAGAGGATTAGCATCCTGACTCCACAATGTCCACCTGCTACTGCTACTGCTACTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

960

Amino Acids

103.94

Weight (kDa)

5.95

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 272 - 491 2.6e-55 Nucleic acid binding protein NABP
NABP PF07990 489 - 615 1.1e-65 Nucleic acid binding protein NABP
PUF PF00806 620 - 654 7.4e-12 Pumilio-family RNA binding repeat
PUF PF00806 657 - 689 4.3e-08 Pumilio-family RNA binding repeat
PUF PF00806 692 - 725 1.8e-09 Pumilio-family RNA binding repeat
PUF PF00806 764 - 799 1.8e-13 Pumilio-family RNA binding repeat
PUF PF00806 802 - 834 1.3e-09 Pumilio-family RNA binding repeat
PUF PF00806 837 - 870 1.9e-07 Pumilio-family RNA binding repeat
PUF PF00806 883 - 912 8.6e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2863
AasI GACNNNNNNGTC 1 cut(s) 1671
Acc16I TGCGCA 1 cut(s) 339
Acc36I ACCTGC 1 cut(s) 2863
Acc65I GGTACC 1 cut(s) 2615
AccB1I GGYRCC 2 cut(s) 579, 2615
AccB7I CCANNNNNTGG 2 cut(s) 842, 1610
AccI GTMKAC 5 cut(s) 213, 1109, 1302, 2245, 2765
AccII CGCG 1 cut(s) 295
AclWI GGATC 7 cut(s) 42, 242, 791, 1053, 1167, 2110, 2354
AcoI YGGCCR 2 cut(s) 1134, 1855
AcsI RAATTY 5 cut(s) 1066, 1312, 1848, 2340, 2534
AcuI CTGAAG 5 cut(s) 530, 615, 2271, 2624, 2777
AcvI CACGTG 1 cut(s) 2792
AfaI GTAC 3 cut(s) 2445, 2617, 2679
AfeI AGCGCT 1 cut(s) 2480
AfiI CCNNNNNNNGG 8 cut(s) 183, 446, 506, 507, 842, 1562, 1610, 2737
AflIII ACRYGT 1 cut(s) 2791
AjnI CCWGG 2 cut(s) 1666, 2589
AjuI GAANNNNNNNTTGG 2 cut(s) 1319, 1351
AloI GAACNNNNNNTCC 2 cut(s) 716, 748
Alw21I GWGCWC 4 cut(s) 144, 240, 1555, 2352
Alw26I GTCTC 2 cut(s) 1288, 1742
AlwI GGATC 7 cut(s) 42, 242, 791, 1053, 1167, 2110, 2354
AlwNI CAGNNNCTG 1 cut(s) 1763
Ama87I CYCGRG 2 cut(s) 500, 736
Aor51HI AGCGCT 1 cut(s) 2480
AoxI GGCC 5 cut(s) 165, 205, 1134, 1855, 2592
ApoI RAATTY 5 cut(s) 1066, 1312, 1848, 2340, 2534
ArsI GACNNNNNNTTYG 2 cut(s) 14, 46
AseI ATTAAT 1 cut(s) 1203
Asp700I GAANNNNTTC 3 cut(s) 856, 2018, 2637
Asp718I GGTACC 1 cut(s) 2615
AspLEI GCGC 4 cut(s) 340, 687, 2481, 2589
AspS9I GGNCC 9 cut(s) 166, 186, 315, 1244, 1664, 1879, 2146, 2593, 2656
AsuC2I CCSGG 2 cut(s) 501, 502
AsuHPI GGTGA 3 cut(s) 425, 1031, 1356
AsuII TTCGAA 1 cut(s) 20
AsuNHI GCTAGC 2 cut(s) 596, 1192
AvaI CYCGRG 2 cut(s) 500, 736
AvaII GGWCC 7 cut(s) 186, 315, 1244, 1664, 1879, 2146, 2656
BalI TGGCCA 2 cut(s) 1136, 1857
BanI GGYRCC 2 cut(s) 579, 2615
BanII GRGCYC 5 cut(s) 144, 240, 820, 1385, 1617
BbrPI CACGTG 1 cut(s) 2792
Bbv12I GWGCWC 4 cut(s) 144, 240, 1555, 2352
BbvCI CCTCAGC 1 cut(s) 801
BccI CCATC 8 cut(s) 476, 611, 1095, 1181, 1366, 1369, 2171, 2377
BceAI ACGGC 3 cut(s) 82, 182, 991
BcgI CGANNNNNNTGC 4 cut(s) 569, 603, 1028, 1062
BciT130I CCWGG 2 cut(s) 1668, 2591
BciVI GTATCC 1 cut(s) 1356
BclI TGATCA 3 cut(s) 2059, 2203, 2287
BcnI CCSGG 2 cut(s) 501, 502
BcoDI GTCTC 2 cut(s) 1288, 1742
BfaI CTAG 5 cut(s) 597, 764, 813, 822, 1193
BfmI CTRYAG 4 cut(s) 930, 1499, 2034, 2127
BfoI RGCGCY 2 cut(s) 2482, 2590
BfuAI ACCTGC 1 cut(s) 2863
BfuI GTATCC 1 cut(s) 1356
BglII AGATCT 1 cut(s) 934
BlpI GCTNAGC 1 cut(s) 2583
Bme1390I CCNGG 4 cut(s) 501, 502, 1668, 2591
Bme18I GGWCC 7 cut(s) 186, 315, 1244, 1664, 1879, 2146, 2656
BmeT110I CYCGRG 2 cut(s) 500, 736
BmgT120I GGNCC 9 cut(s) 166, 186, 315, 1244, 1664, 1879, 2146, 2593, 2656
BmrFI CCNGG 4 cut(s) 501, 502, 1668, 2591
BmrI ACTGGG 5 cut(s) 634, 1099, 1397, 2079, 2514
BmsI GCATC 9 cut(s) 642, 664, 667, 915, 972, 1799, 2245, 2598, 2841
BmtI GCTAGC 2 cut(s) 600, 1196
BmuI ACTGGG 5 cut(s) 634, 1099, 1397, 2079, 2514
BpmI CTGGAG 3 cut(s) 1479, 2477, 2835
Bpu10I CCTNAGC 2 cut(s) 801, 2087
Bpu1102I GCTNAGC 1 cut(s) 2583
Bpu14I TTCGAA 1 cut(s) 20
BpuEI CTTGAG 4 cut(s) 41, 559, 1258, 2735
BpuMI CCSGG 2 cut(s) 501, 502
BsaAI YACGTR 3 cut(s) 15, 897, 2792
BsaBI GATNNNNATC 1 cut(s) 2831
BsaI GGTCTC 1 cut(s) 1742
BsaJI CCNNGG 7 cut(s) 228, 293, 318, 500, 735, 827, 1659
BsaWI WCCGGW 1 cut(s) 240
BsaXI ACNNNNNCTCC 6 cut(s) 227, 257, 317, 347, 394, 424
Bsc4I CCNNNNNNNGG 8 cut(s) 183, 446, 506, 507, 842, 1562, 1610, 2737
Bse3DI GCAATG 2 cut(s) 1446, 2652
Bse8I GATNNNNATC 1 cut(s) 2831
BseBI CCWGG 2 cut(s) 1668, 2591
BseDI CCNNGG 7 cut(s) 228, 293, 318, 500, 735, 827, 1659
BseJI GATNNNNATC 1 cut(s) 2831
BseLI CCNNNNNNNGG 8 cut(s) 183, 446, 506, 507, 842, 1562, 1610, 2737
BseMI GCAATG 2 cut(s) 1446, 2652
BseMII CTCAG 7 cut(s) 662, 815, 1293, 1770, 2496, 2574, 2595
BseRI GAGGAG 7 cut(s) 245, 248, 338, 372, 492, 861, 1485
BseYI CCCAGC 3 cut(s) 592, 1340, 1492
BsgI GTGCAG 1 cut(s) 455
Bsh1236I CGCG 1 cut(s) 295
BshFI GGCC 5 cut(s) 167, 207, 1136, 1857, 2594
BshNI GGYRCC 2 cut(s) 579, 2615
BsiHKAI GWGCWC 4 cut(s) 144, 240, 1555, 2352
BsiHKCI CYCGRG 2 cut(s) 500, 736
BsiSI CCGG 5 cut(s) 241, 251, 440, 501, 601
BslFI GGGAC 4 cut(s) 172, 1069, 1530, 1677
BslI CCNNNNNNNGG 8 cut(s) 183, 446, 506, 507, 842, 1562, 1610, 2737
BsmAI GTCTC 2 cut(s) 1288, 1742
BsmFI GGGAC 4 cut(s) 172, 1069, 1530, 1677
BsmI GAATGC 3 cut(s) 610, 1093, 2755
BsnI GGCC 5 cut(s) 167, 207, 1136, 1857, 2594
Bso31I GGTCTC 1 cut(s) 1742
BsoBI CYCGRG 2 cut(s) 500, 736
Bsp119I TTCGAA 1 cut(s) 20
Bsp1286I GDGCHC 7 cut(s) 144, 240, 820, 1385, 1555, 1617, 2352
Bsp1407I TGTACA 2 cut(s) 2443, 2677
Bsp1720I GCTNAGC 1 cut(s) 2583
BspANI GGCC 5 cut(s) 167, 207, 1136, 1857, 2594
BspCNI CTCAG 7 cut(s) 661, 814, 1292, 1769, 2495, 2575, 2596
BspFNI CGCG 1 cut(s) 295
BspMAI CTGCAG 3 cut(s) 934, 1503, 2038
BspMI ACCTGC 1 cut(s) 2863
BspOI GCTAGC 2 cut(s) 600, 1196
BspPI GGATC 7 cut(s) 42, 242, 791, 1053, 1167, 2110, 2354
BspQI GCTCTTC 1 cut(s) 847
BspT104I TTCGAA 1 cut(s) 20
BspT107I GGYRCC 2 cut(s) 579, 2615
BspTNI GGTCTC 1 cut(s) 1742
BsrDI GCAATG 2 cut(s) 1446, 2652
BsrGI TGTACA 2 cut(s) 2443, 2677
BssECI CCNNGG 7 cut(s) 228, 293, 318, 500, 735, 827, 1659
BssNAI GTATAC 2 cut(s) 2246, 2766
BssT1I CCWWGG 1 cut(s) 318
Bst1107I GTATAC 2 cut(s) 2246, 2766
Bst2UI CCWGG 2 cut(s) 1668, 2591
Bst4CI ACNGT 7 cut(s) 80, 178, 777, 841, 1545, 2304, 2732
Bst6I CTCTTC 3 cut(s) 521, 847, 984
BstAUI TGTACA 2 cut(s) 2443, 2677
BstBAI YACGTR 3 cut(s) 15, 897, 2792
BstBI TTCGAA 1 cut(s) 20
BstC8I GCNNGC 4 cut(s) 598, 1194, 2400, 2528
BstDEI CTNAG 9 cut(s) 648, 801, 1279, 1756, 2087, 2482, 2583, 2604, 2877
BstDSI CCRYGG 2 cut(s) 293, 1659
BstFNI CGCG 1 cut(s) 295
BstH2I RGCGCY 2 cut(s) 2482, 2590
BstHHI GCGC 4 cut(s) 340, 687, 2481, 2589
BstMAI GTCTC 2 cut(s) 1288, 1742
BstNI CCWGG 2 cut(s) 1668, 2591
BstNSI RCATGY 5 cut(s) 1480, 1624, 2078, 2402, 2453
BstSCI CCNGG 4 cut(s) 499, 500, 1666, 2589
BstSFI CTRYAG 4 cut(s) 930, 1499, 2034, 2127
BstSNI TACGTA 2 cut(s) 15, 897
BstUI CGCG 1 cut(s) 295
BstX2I RGATCY 2 cut(s) 796, 934
BstYI RGATCY 2 cut(s) 796, 934
BstZ17I GTATAC 2 cut(s) 2246, 2766
BsuI GTATCC 1 cut(s) 1356
BsuRI GGCC 5 cut(s) 167, 207, 1136, 1857, 2594
BtgI CCRYGG 2 cut(s) 293, 1659
BtsI GCAGTG 1 cut(s) 2350
BveI ACCTGC 1 cut(s) 2863
Cac8I GCNNGC 4 cut(s) 598, 1194, 2400, 2528
CaiI CAGNNNCTG 1 cut(s) 1763
CfoI GCGC 4 cut(s) 340, 687, 2481, 2589
Cfr13I GGNCC 9 cut(s) 166, 186, 315, 1244, 1664, 1879, 2146, 2593, 2656
Cfr42I CCGCGG 1 cut(s) 296
Cfr9I CCCGGG 1 cut(s) 500
CsiI ACCWGGT 1 cut(s) 1666
Csp6I GTAC 3 cut(s) 2444, 2616, 2678
CspCI CAANNNNNGTGG 2 cut(s) 1126, 1161
CviQI GTAC 3 cut(s) 2444, 2616, 2678
DdeI CTNAG 9 cut(s) 648, 801, 1279, 1756, 2087, 2482, 2583, 2604, 2877
DrdI GACNNNNNNGTC 1 cut(s) 1671
DseDI GACNNNNNNGTC 1 cut(s) 1671
EaeI YGGCCR 2 cut(s) 1134, 1855
Eam1104I CTCTTC 3 cut(s) 521, 847, 984
EarI CTCTTC 3 cut(s) 521, 847, 984
EciI GGCGGA 1 cut(s) 369
Ecl136II GAGCTC 2 cut(s) 142, 238
Eco105I TACGTA 2 cut(s) 15, 897
Eco130I CCWWGG 1 cut(s) 318
Eco24I GRGCYC 5 cut(s) 144, 240, 820, 1385, 1617
Eco31I GGTCTC 1 cut(s) 1742
Eco47I GGWCC 7 cut(s) 186, 315, 1244, 1664, 1879, 2146, 2656
Eco47III AGCGCT 1 cut(s) 2480
Eco53kI GAGCTC 2 cut(s) 142, 238
Eco57I CTGAAG 5 cut(s) 530, 615, 2271, 2624, 2777
Eco72I CACGTG 1 cut(s) 2792
Eco88I CYCGRG 2 cut(s) 500, 736
EcoICRI GAGCTC 2 cut(s) 142, 238
EcoO109I RGGNCCY 3 cut(s) 186, 1244, 2593
EcoRI GAATTC 1 cut(s) 1312
EcoRII CCWGG 2 cut(s) 1666, 2589
EcoT14I CCWWGG 1 cut(s) 318
EcoT22I ATGCAT 2 cut(s) 657, 987
EcoT38I GRGCYC 5 cut(s) 144, 240, 820, 1385, 1617
ErhI CCWWGG 1 cut(s) 318
FalI AAGNNNNNCTT 8 cut(s) 408, 440, 568, 600, 844, 876, 981, 1013
FaqI GGGAC 4 cut(s) 172, 1069, 1530, 1677
FauI CCCGC 2 cut(s) 336, 392
FauNDI CATATG 2 cut(s) 1321, 2317
FbaI TGATCA 3 cut(s) 2059, 2203, 2287
FblI GTMKAC 5 cut(s) 213, 1109, 1302, 2245, 2765
FriOI GRGCYC 5 cut(s) 144, 240, 820, 1385, 1617
FspBI CTAG 5 cut(s) 597, 764, 813, 822, 1193
FspI TGCGCA 1 cut(s) 339
GlaI GCGC 4 cut(s) 339, 686, 2480, 2588
GsaI CCCAGC 3 cut(s) 596, 1344, 1496
GsuI CTGGAG 3 cut(s) 1479, 2477, 2835
HaeII RGCGCY 2 cut(s) 2482, 2590
HaeIII GGCC 5 cut(s) 167, 207, 1136, 1857, 2594
HapII CCGG 5 cut(s) 241, 251, 440, 501, 601
HhaI GCGC 4 cut(s) 340, 687, 2481, 2589
Hin6I GCGC 4 cut(s) 338, 685, 2479, 2587
HinP1I GCGC 4 cut(s) 338, 685, 2479, 2587
HincII GTYRAC 2 cut(s) 223, 2307
HindII GTYRAC 2 cut(s) 223, 2307
HindIII AAGCTT 2 cut(s) 1506, 1910
HpaI GTTAAC 1 cut(s) 223
HpaII CCGG 5 cut(s) 241, 251, 440, 501, 601
HphI GGTGA 3 cut(s) 425, 1031, 1356
Hpy166II GTNNAC 9 cut(s) 214, 223, 773, 1110, 1303, 2246, 2307, 2766, 2852
Hpy8I GTNNAC 9 cut(s) 214, 223, 773, 1110, 1303, 2246, 2307, 2766, 2852
Hpy99I CGWCG 2 cut(s) 203, 221
HpyCH4III ACNGT 7 cut(s) 80, 178, 777, 841, 1545, 2304, 2732
HpyCH4IV ACGT 5 cut(s) 14, 219, 896, 1702, 2791
HpyF3I CTNAG 9 cut(s) 648, 801, 1279, 1756, 2087, 2482, 2583, 2604, 2877
HpySE526I ACGT 5 cut(s) 14, 219, 896, 1702, 2791
HspAI GCGC 4 cut(s) 338, 685, 2479, 2587
KflI GGGWCCC 1 cut(s) 186
KpnI GGTACC 1 cut(s) 2619
Ksp22I TGATCA 3 cut(s) 2059, 2203, 2287
KspAI GTTAAC 1 cut(s) 223
KspI CCGCGG 1 cut(s) 296
LguI GCTCTTC 1 cut(s) 847
LweI GCATC 9 cut(s) 642, 664, 667, 915, 972, 1799, 2245, 2598, 2841
MabI ACCWGGT 1 cut(s) 1666
MaeI CTAG 5 cut(s) 597, 764, 813, 822, 1193
MaeII ACGT 5 cut(s) 14, 219, 896, 1702, 2791
MaeIII GTNAC 8 cut(s) 4, 125, 1362, 1539, 1688, 2164, 2296, 2599
MfeI CAATTG 1 cut(s) 1520
MflI RGATCY 2 cut(s) 796, 934
MhlI GDGCHC 7 cut(s) 144, 240, 820, 1385, 1555, 1617, 2352
MlsI TGGCCA 2 cut(s) 1136, 1857
MluNI TGGCCA 2 cut(s) 1136, 1857
MlyI GAGTC 6 cut(s) 100, 935, 1448, 1900, 2149, 2833
MmeI TCCRAC 3 cut(s) 352, 823, 860
Mox20I TGGCCA 2 cut(s) 1136, 1857
Mph1103I ATGCAT 2 cut(s) 657, 987
MroXI GAANNNNTTC 3 cut(s) 856, 2018, 2637
MscI TGGCCA 2 cut(s) 1136, 1857
MseI TTAA 6 cut(s) 222, 1203, 1263, 2564, 2727, 2736
MslI CAYNNNNRTG 6 cut(s) 750, 1481, 2316, 2355, 2606, 2748
Msp20I TGGCCA 2 cut(s) 1136, 1857
MspA1I CMGCKG 2 cut(s) 295, 343
MspI CCGG 5 cut(s) 241, 251, 440, 501, 601
MspR9I CCNGG 4 cut(s) 501, 502, 1668, 2591
MunI CAATTG 1 cut(s) 1520
Mva1269I GAATGC 3 cut(s) 610, 1093, 2755
MvaI CCWGG 2 cut(s) 1668, 2591
MvnI CGCG 1 cut(s) 295
NciI CCSGG 2 cut(s) 501, 502
NdeI CATATG 2 cut(s) 1321, 2317
NheI GCTAGC 2 cut(s) 596, 1192
NmeAIII GCCGAG 1 cut(s) 1596
NmuCI GTSAC 7 cut(s) 125, 1362, 1539, 1688, 2164, 2296, 2599
NsbI TGCGCA 1 cut(s) 339
NsiI ATGCAT 2 cut(s) 657, 987
NspI RCATGY 5 cut(s) 1480, 1624, 2078, 2402, 2453
NspV TTCGAA 1 cut(s) 20
PaeI GCATGC 1 cut(s) 2402
PaqCI CACCTGC 1 cut(s) 2863
PciSI GCTCTTC 1 cut(s) 847
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PctI GAATGC 3 cut(s) 610, 1093, 2755
PdmI GAANNNNTTC 3 cut(s) 856, 2018, 2637
PfeI GAWTC 8 cut(s) 100, 541, 832, 994, 1012, 1582, 2008, 2392
PflFI GACNNNGTC 1 cut(s) 2302
PflMI CCANNNNNTGG 2 cut(s) 842, 1610
PleI GAGTC 6 cut(s) 99, 934, 1448, 1899, 2149, 2833
PmaCI CACGTG 1 cut(s) 2792
PmlI CACGTG 1 cut(s) 2792
PpsI GAGTC 6 cut(s) 99, 934, 1448, 1899, 2149, 2833
Ppu21I YACGTR 3 cut(s) 15, 897, 2792
PpuMI RGGWCCY 2 cut(s) 186, 1244
PshBI ATTAAT 1 cut(s) 1203
Psp124BI GAGCTC 2 cut(s) 144, 240
Psp5II RGGWCCY 2 cut(s) 186, 1244
Psp6I CCWGG 2 cut(s) 1666, 2589
PspCI CACGTG 1 cut(s) 2792
PspFI CCCAGC 3 cut(s) 592, 1340, 1492
PspGI CCWGG 2 cut(s) 1666, 2589
PspPI GGNCC 9 cut(s) 166, 186, 315, 1244, 1664, 1879, 2146, 2593, 2656
PspPPI RGGWCCY 2 cut(s) 186, 1244
PstI CTGCAG 3 cut(s) 934, 1503, 2038
PstNI CAGNNNCTG 1 cut(s) 1763
PsuI RGATCY 2 cut(s) 796, 934
PsyI GACNNNGTC 1 cut(s) 2302
RsaI GTAC 3 cut(s) 2445, 2617, 2679
RsaNI GTAC 3 cut(s) 2444, 2616, 2678
RseI CAYNNNNRTG 6 cut(s) 750, 1481, 2316, 2355, 2606, 2748
SacI GAGCTC 2 cut(s) 144, 240
SacII CCGCGG 1 cut(s) 296
SapI GCTCTTC 1 cut(s) 847
SaqAI TTAA 6 cut(s) 222, 1203, 1263, 2564, 2727, 2736
Sau96I GGNCC 9 cut(s) 166, 186, 315, 1244, 1664, 1879, 2146, 2593, 2656
SchI GAGTC 6 cut(s) 100, 935, 1448, 1900, 2149, 2833
ScrFI CCNGG 4 cut(s) 501, 502, 1668, 2591
SduI GDGCHC 7 cut(s) 144, 240, 820, 1385, 1555, 1617, 2352
SexAI ACCWGGT 1 cut(s) 1666
SfaNI GCATC 9 cut(s) 642, 664, 667, 915, 972, 1799, 2245, 2598, 2841
SfcI CTRYAG 4 cut(s) 930, 1499, 2034, 2127
Sfr303I CCGCGG 1 cut(s) 296
SfuI TTCGAA 1 cut(s) 20
SgrBI CCGCGG 1 cut(s) 296
SinI GGWCC 7 cut(s) 186, 315, 1244, 1664, 1879, 2146, 2656
SmaI CCCGGG 1 cut(s) 502
SmiMI CAYNNNNRTG 6 cut(s) 750, 1481, 2316, 2355, 2606, 2748
SmlI CTYRAG 4 cut(s) 56, 574, 1237, 2714
SmoI CTYRAG 4 cut(s) 56, 574, 1237, 2714
SnaBI TACGTA 2 cut(s) 15, 897
SphI GCATGC 1 cut(s) 2402
SspMI CTAG 5 cut(s) 597, 764, 813, 822, 1193
SstI GAGCTC 2 cut(s) 144, 240
StyD4I CCNGG 4 cut(s) 499, 500, 1666, 2589
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 7 cut(s) 80, 178, 777, 841, 1545, 2304, 2732
TaiI ACGT 5 cut(s) 17, 222, 899, 1705, 2794
TaqI TCGA 5 cut(s) 20, 154, 1776, 2022, 2110
TaqII GACCGA 1 cut(s) 2000
TatI WGTACW 2 cut(s) 2443, 2677
TauI GCSGC 9 cut(s) 72, 207, 298, 304, 307, 351, 354, 455, 458
TfiI GAWTC 8 cut(s) 100, 541, 832, 994, 1012, 1582, 2008, 2392
Tru1I TTAA 6 cut(s) 222, 1203, 1263, 2564, 2727, 2736
Tru9I TTAA 6 cut(s) 222, 1203, 1263, 2564, 2727, 2736
TseFI GTSAC 7 cut(s) 125, 1362, 1539, 1688, 2164, 2296, 2599
Tsp45I GTSAC 7 cut(s) 125, 1362, 1539, 1688, 2164, 2296, 2599
TspMI CCCGGG 1 cut(s) 500
Tth111I GACNNNGTC 1 cut(s) 2302
Van91I CCANNNNNTGG 2 cut(s) 842, 1610
VpaK11BI GGWCC 7 cut(s) 186, 315, 1244, 1664, 1879, 2146, 2656
VspI ATTAAT 1 cut(s) 1203
XapI RAATTY 5 cut(s) 1066, 1312, 1848, 2340, 2534
XceI RCATGY 5 cut(s) 1480, 1624, 2078, 2402, 2453
XmaI CCCGGG 1 cut(s) 500
XmiI GTMKAC 5 cut(s) 213, 1109, 1302, 2245, 2765
XmnI GAANNNNTTC 3 cut(s) 856, 2018, 2637
XspI CTAG 5 cut(s) 597, 764, 813, 822, 1193
Zsp2I ATGCAT 2 cut(s) 657, 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.