Rh5BG451000

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002776
Physical Location & Seq
Forward (+)
72443137 .. 72449999
6863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG451000.1

Sequence Viewer

Length: 3276 bp
ATGTTGTCTGAATTGGGGAGAAGGCCAATGCTTGGAGGTAACGAGGGTTCGTTTGGGGATGAATTTGAGAAGGAGATTGGTATGTTGCTTAGGGAGCAGCGTCGGCAGGAGGCTGATGATCGTGAGAGTGACCTGAATATTTACAGGAGTGGGTCGGCGCCGCCGACTGTGGAGGGTTCTTTGAATGCGGTGGGAGGGTTGTTTGCTGGCGGTGGAAGTGGAGGTGGAGGGGGAGGGGGAGGGGGAGGGGGAGGGGGAGGAGGGGGTGTTGGCGGTGGTGGGGGTGCTGGGTCTGTTCTTTCGGAGTTTCCTGGAGCTAAGAATGGGAATGGGTTTGCGTCGGAGGAGGAGCTGAGGTCTGATCCAGCTTATCTGCAATACTATTACTCGAATGTGAATCTGAATCCGAGGCTTCCGCCTCCTTTGTTGTCGAAGGAGGATTGGAGGTATGCACAGAGGATGAAAGGAGGGAGTTCTGTTTTGGGTGGGATTGGGGACAGGAGGAAAGTGAACAGGGCTGATGATGCTAGTGGTGGGAGATCCATGTTTTCGATGCCGCCAGGGTTTAACTCGAGGAAACAAGAGAGTGAGGTGGAGCCGGACAAAGTGCGTGGCTCAGCGGAGTGGGGTGTTGATGGGCTAATCGGTTTGCCAGGTTTAGGACTTGGCAACAAGCAAAAAAGCCTTGCTGAAATCTTTCAGGATGACTTGGGGCGTACGACTCCTGTTCCTGGGCTTCCTTCTCGTCCAGCCAGCCGTAATGCATTTGATGAAAATGTTGAGGGACTTGGTTCTGCTGAAGCAGACCTGGCTCATTTGCGCCGTGACTTGATGACATCTGATGCTCTAAGATCTGGTGCAAATGGCCAGGGCATGTCTGCAGCCCAAAGCATGGGCCCACCTTCTTCATACTCCTATGCTGCAGCTCTAGGTGCTTCCTTGTCAAGAAGCACCACTCCTGATCCTCAGATTGTTGCTAGGGCTCCTAGTCCTTGCCTTACACCTATTGGAGGAGGAAGAGTTAGTGCTTCAGAAAAGAGAGGCATTTCTAGTCCAAGCTCCTTTAATGCTGTCTCATCTGGCATTAATGAGTCTGGAGATCTGGTAGCTGCCTTATCCACCATGAATTTGTCTTCAAATGGTGTAATAGATGATGATCACCATTTGCCATCACAGATCAAACAGGATGTGATTGATCACCAGAATTATCTCTTTGGTCTGCAAGGTGCTGATAGTCATGCTAAGCAACTTGCTTACTTAAAGAAGTCTGAATCTGGGAATATACATATGCCTTCGCCACAGTCGGCAAAAGGTTCGTACTCGGATTTGGGTAAGAGCAATGGGGGTGGGTCAGATCTGAACAACTCATCCCCTGACAGGCAAGTTGAGCTGCAAAAATCTGCTGTTCCTTCTAGTAACTTGTATAAAGGATCATCTGCATCGAATCTTAATGGTGGAGGTGGATTGCATAATCAGTATCAGCAAGTGGATAATGCAAATTCATCATTTTCTAACTATGGTTTAAGTGGCTACTCTATGAATCCAGCCTTGGCATCCATGGTGGCTAGCCAACTTGGTACTGGTAATCTGCCTCCTCTGTTTGAAAATGTTGCTGCAGCATCAGCTATGGTACCTCCTGGAATGGATTCAAGAGTACTTGGAGGGGGTTTGGCTTCTGGACCAAACCTAGCAGCTGCTGCATCTGATTCACATAATCTTGGTAGATTAGGGAGTCCGATTGCGGGGACCGGTCTCCAGGCGCCTTATGTTGATCCTATGTATCTTCAATACTTGAGGACATCAGAGTATGCTGCAGCACAGCTTGCTGCCCTGAATGATCCTTCTGTGGATAGGAACTACTTAGGTAATTCATACATGAATTTACTTGAACTTCAAAAGGCGTATCTTGGAGCCTTGCTGTCACCTCAGAAGTCACAGTATGGTGTTGGTGTTCCCTTGGGGGGTAAGACTGGTGGTTCAAATCATCATGGTTATTATGGAAATCATGCCTTTGGCATGTCTTATCCAGGAAGTCCCATGGCAAGTCCCGTTATTCCCAACTCTCCAGTTGCCCCTGGTAGTCCAATGAGGCACAATGATCTTAATATGTGTTATCCTTCTGGGATGAGGAATTTGAATTTGGGTGGGAGTGTCATGGGACCATGGCATCTGGATTCTGGTTGTAACATAGATGAGACCTTTGCATCATCACTCTTAGAAGAGTTTAAGAGCAACAAAGCCAAGTCTTTTGAACTTTTGGAAATCGGAGGACATGTTGTGGAATTCAGTGCGGATCAATACGGGAGTCGGTTTATTCAACAAAAACTTGAAACAGCCACTACGGATGAGAAAAACATGGTTTACCAGGAAATCATGCCTCAAGCTCTTGCTCTAATGACTGATGTGTTTGGTAATTATGTGATTCAAAAGTTTTTTGAGCACGGGCTTCCATCTCAAAGAAGGGAATTGGCCAACAAGCTTTTTGGTCATGTGCTGACACTTAGCCTTCAAATGTATGGTTGTCGGGTGATACAGAAGGCAATAGAAGTTGTTGATCTGGACCAGAAGATCAAAATGGTTGAAGAACTTGACGGCCATGTCATGCGCTGTGTGCGTGACCAGAATGGGAACCATGTCATTCAGAAGTGTATTGAATGTGTCCCTGAAGAGGCCATTCATTTTATAGTCTCAACGTTTTTTGATCAAGTTGTGACCCTGTCCACTCATCCATATGGGTGTCGTGTGATACAGAGAGTGCTTGAGCACTGCAATGACCAGAATACTCAGAGCAAAGTTATGGATGAGATTTTAGGAGCTGTTAGCATGCTGGCACAAGATCAGTATGGCAATTATGTTGTTCAGCATGTACTGGAGCATGGAAAGCCACATGAGCGTTCTGCTATAATAAAGGAATTAGCTGGGAAGATAGTCCAAATGAGTCAACAGAAGTTTGCCTCTAATGTTGTAGAGAAATGCTTAGCTTTTGGTGGTCCTGCTGAAAGGGAATTACTAGTGAATGAGATGCTTGGCACTACTGATGAAAACGAGCCTCTTCAGGCAATGATGAAAGATCAGTTTGCAAACTACGTTGTACAGAAAGTGTTGGAGACTTGTGATGACCAACAACGCGAGCTGATTCTTTCACGAATTAAAGTTCATTTGAATGCATTGAAGAAATACACTTACGGGAAGCATATCGTGGCTCGTGTAGAAAAACTTGTTGCTGCTGGGGAAAGGAGAGTTGCTGCTGCTCAGTTGGCTCCTCACCCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1091

Amino Acids

116.19

Weight (kDa)

5.83

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 327 - 752 8.2e-172 Nucleic acid binding protein NABP
PUF PF00806 758 - 792 2.9e-10 Pumilio-family RNA binding repeat
PUF PF00806 796 - 827 1.3e-07 Pumilio-family RNA binding repeat
PUF PF00806 830 - 863 1.8e-09 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 866 - 947 1.6e-07 NOP9-like PUF repeat domain
PUF PF00806 872 - 896 1e-05 Pumilio-family RNA binding repeat
PUF PF00806 902 - 937 7.4e-13 Pumilio-family RNA binding repeat
PUF PF00806 942 - 972 8.5e-10 Pumilio-family RNA binding repeat
PUF PF00806 976 - 1009 4.2e-07 Pumilio-family RNA binding repeat
PUF PF00806 1021 - 1050 2e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2597
Acc65I GGTACC 1 cut(s) 1630
AccB1I GGYRCC 3 cut(s) 157, 1630, 1759
AccB7I CCANNNNNTGG 2 cut(s) 32, 892
AccII CGCG 1 cut(s) 3129
AciI CCGC 9 cut(s) 161, 188, 210, 273, 416, 557, 620, 1742, 2291
AclI AACGTT 1 cut(s) 2693
AclWI GGATC 7 cut(s) 356, 534, 956, 1438, 1766, 1832, 2301
AcoI YGGCCR 3 cut(s) 865, 2469, 2593
AcsI RAATTY 7 cut(s) 62, 1126, 1498, 1879, 2131, 2137, 2282
AcuI CTGAAG 4 cut(s) 819, 1014, 2685, 3038
AcyI GRCGYC 2 cut(s) 158, 1760
AfaI GTAC 7 cut(s) 718, 1319, 1579, 1632, 1656, 2868, 3093
AfiI CCNNNNNNNGG 9 cut(s) 32, 659, 731, 892, 1010, 1377, 1742, 1961, 2668
AflIII ACRYGT 1 cut(s) 2272
AgeI ACCGGT 1 cut(s) 1748
AhlI ACTAGT 1 cut(s) 3010
AjuI GAANNNNNNNTTGG 4 cut(s) 1532, 1564, 2123, 2155
AloI GAACNNNNNNTCC 2 cut(s) 1352, 1384
Alw21I GWGCWC 2 cut(s) 2442, 2766
Alw26I GTCTC 5 cut(s) 1078, 1757, 2189, 2692, 3101
AlwI GGATC 7 cut(s) 356, 534, 956, 1438, 1766, 1832, 2301
AlwNI CAGNNNCTG 1 cut(s) 1697
Ama87I CYCGRG 1 cut(s) 571
AoxI GGCC 6 cut(s) 23, 865, 895, 2469, 2593, 2670
ApaI GGGCCC 1 cut(s) 899
ApoI RAATTY 7 cut(s) 62, 1126, 1498, 1879, 2131, 2137, 2282
AseI ATTAAT 1 cut(s) 1086
AsiGI ACCGGT 1 cut(s) 1748
Asp700I GAANNNNTTC 2 cut(s) 696, 1645
Asp718I GGTACC 1 cut(s) 1630
AspLEI GCGC 4 cut(s) 160, 822, 1762, 2607
AspS9I GGNCC 7 cut(s) 895, 896, 1679, 1746, 2159, 2560, 2990
AsuHPI GGTGA 5 cut(s) 1151, 1190, 1914, 2539, 3257
AsuNHI GCTAGC 1 cut(s) 1565
AvaI CYCGRG 1 cut(s) 571
AvaII GGWCC 5 cut(s) 1679, 1746, 2159, 2560, 2990
BaeGI GKGCMC 1 cut(s) 899
BalI TGGCCA 2 cut(s) 867, 2471
BanI GGYRCC 3 cut(s) 157, 1630, 1759
BanII GRGCYC 2 cut(s) 899, 985
BauI CACGAG 1 cut(s) 3204
BbsI GAAGAC 1 cut(s) 1125
Bbv12I GWGCWC 2 cut(s) 2442, 2766
BbvCI CCTCAGC 1 cut(s) 353
BccI CCATC 3 cut(s) 629, 1177, 2458
BceAI ACGGC 3 cut(s) 741, 807, 2608
BcgI CGANNNNNNTGC 2 cut(s) 1296, 1330
BclI TGATCA 3 cut(s) 1156, 1195, 2701
BcoDI GTCTC 5 cut(s) 1078, 1757, 2189, 2692, 3101
BcuI ACTAGT 1 cut(s) 3010
BfaI CTAG 9 cut(s) 528, 929, 978, 987, 1050, 1413, 1566, 1688, 3011
BfmI CTRYAG 4 cut(s) 879, 921, 1614, 1812
BfoI RGCGCY 2 cut(s) 161, 1763
BglII AGATCT 3 cut(s) 851, 1099, 1354
BlpI GCTNAGC 3 cut(s) 616, 1242, 2977
BmcAI AGTACT 1 cut(s) 1656
Bme18I GGWCC 5 cut(s) 1679, 1746, 2159, 2560, 2990
BmeT110I CYCGRG 1 cut(s) 571
BmgT120I GGNCC 7 cut(s) 895, 896, 1679, 1746, 2159, 2560, 2990
BmtI GCTAGC 1 cut(s) 1569
BpiI GAAGAC 1 cut(s) 1125
BpmI CTGGAG 5 cut(s) 333, 1116, 1739, 2049, 2891
Bpu10I CCTNAGC 2 cut(s) 89, 353
Bpu1102I GCTNAGC 3 cut(s) 616, 1242, 2977
BpuEI CTTGAG 3 cut(s) 1813, 2364, 2780
BsaBI GATNNNNATC 1 cut(s) 1155
BsaHI GRCGYC 2 cut(s) 158, 1760
BsaI GGTCTC 2 cut(s) 1757, 2189
BsaWI WCCGGW 1 cut(s) 1748
Bsc4I CCNNNNNNNGG 9 cut(s) 32, 659, 731, 892, 1010, 1377, 1742, 1961, 2668
Bse118I RCCGGY 1 cut(s) 1748
Bse1I ACTGG 4 cut(s) 1585, 1975, 2066, 2874
Bse3DI GCAATG 3 cut(s) 1345, 2776, 3066
Bse8I GATNNNNATC 1 cut(s) 1155
BseJI GATNNNNATC 1 cut(s) 1155
BseLI CCNNNNNNNGG 9 cut(s) 32, 659, 731, 892, 1010, 1377, 1742, 1961, 2668
BseMI GCAATG 3 cut(s) 1345, 2776, 3066
BseMII CTCAG 6 cut(s) 344, 630, 980, 1940, 2798, 3266
BseNI ACTGG 4 cut(s) 1585, 1975, 2066, 2874
BseRI GAGGAG 6 cut(s) 273, 359, 362, 1026, 1584, 3252
BseSI GKGCMC 1 cut(s) 899
BseYI CCCAGC 3 cut(s) 287, 2918, 3227
Bsh1236I CGCG 1 cut(s) 3129
BshFI GGCC 6 cut(s) 25, 867, 897, 2471, 2595, 2672
BshNI GGYRCC 3 cut(s) 157, 1630, 1759
BshTI ACCGGT 1 cut(s) 1748
BsiHKAI GWGCWC 2 cut(s) 2442, 2766
BsiHKCI CYCGRG 1 cut(s) 571
BsiSI CCGG 2 cut(s) 599, 1749
BsiWI CGTACG 1 cut(s) 716
BslFI GGGAC 7 cut(s) 509, 798, 1759, 2019, 2031, 2172, 2645
BslI CCNNNNNNNGG 9 cut(s) 32, 659, 731, 892, 1010, 1377, 1742, 1961, 2668
BsmAI GTCTC 5 cut(s) 1078, 1757, 2189, 2692, 3101
BsmFI GGGAC 7 cut(s) 509, 798, 1759, 2019, 2031, 2172, 2645
BsmI GAATGC 2 cut(s) 190, 3169
BsnI GGCC 6 cut(s) 25, 867, 897, 2471, 2595, 2672
Bso31I GGTCTC 2 cut(s) 1757, 2189
BsoBI CYCGRG 1 cut(s) 571
Bsp120I GGGCCC 1 cut(s) 895
Bsp1286I GDGCHC 4 cut(s) 899, 985, 2442, 2766
Bsp1407I TGTACA 1 cut(s) 3091
Bsp1720I GCTNAGC 3 cut(s) 616, 1242, 2977
Bsp19I CCATGG 3 cut(s) 1557, 2037, 2162
BspACI CCGC 9 cut(s) 161, 188, 210, 273, 416, 557, 620, 1742, 2291
BspANI GGCC 6 cut(s) 25, 867, 897, 2471, 2595, 2672
BspCNI CTCAG 6 cut(s) 345, 629, 979, 1939, 2797, 3265
BspFNI CGCG 1 cut(s) 3129
BspMAI CTGCAG 4 cut(s) 883, 925, 1618, 1816
BspOI GCTAGC 1 cut(s) 1569
BspPI GGATC 7 cut(s) 356, 534, 956, 1438, 1766, 1832, 2301
BspT107I GGYRCC 3 cut(s) 157, 1630, 1759
BspTNI GGTCTC 2 cut(s) 1757, 2189
BsrDI GCAATG 3 cut(s) 1345, 2776, 3066
BsrFI RCCGGY 1 cut(s) 1748
BsrGI TGTACA 1 cut(s) 3091
BsrI ACTGG 4 cut(s) 1585, 1975, 2066, 2874
BssAI RCCGGY 1 cut(s) 1748
BssNI GRCGYC 2 cut(s) 158, 1760
BssSI CACGAG 1 cut(s) 3204
BssT1I CCWWGG 5 cut(s) 1548, 1557, 1956, 2037, 2162
Bst2BI CACGAG 1 cut(s) 3204
Bst4CI ACNGT 3 cut(s) 169, 1302, 1938
Bst6I CTCTTC 4 cut(s) 1012, 2214, 2661, 3057
BstACI GRCGYC 2 cut(s) 158, 1760
BstAPI GCANNNNNTGC 2 cut(s) 1697, 1823
BstAUI TGTACA 1 cut(s) 3091
BstC8I GCNNGC 7 cut(s) 208, 754, 1567, 1824, 2825, 2829, 3131
BstDSI CCRYGG 3 cut(s) 1557, 2037, 2162
BstENI CCTNNNNNAGG 1 cut(s) 1008
BstFNI CGCG 1 cut(s) 3129
BstH2I RGCGCY 2 cut(s) 161, 1763
BstHHI GCGC 4 cut(s) 160, 822, 1762, 2607
BstMAI GTCTC 5 cut(s) 1078, 1757, 2189, 2692, 3101
BstNSI RCATGY 5 cut(s) 877, 2020, 2276, 2827, 2867
BstSFI CTRYAG 4 cut(s) 879, 921, 1614, 1812
BstSLI GKGCMC 1 cut(s) 899
BstUI CGCG 1 cut(s) 3129
BstV2I GAAGAC 1 cut(s) 1125
BstX2I RGATCY 4 cut(s) 539, 851, 1099, 1354
BstYI RGATCY 4 cut(s) 539, 851, 1099, 1354
BsuRI GGCC 6 cut(s) 25, 867, 897, 2471, 2595, 2672
BtgI CCRYGG 3 cut(s) 1557, 2037, 2162
BtsI GCAGTG 1 cut(s) 2764
BtsIMutI CAGTG 2 cut(s) 2293, 2764
Cac8I GCNNGC 7 cut(s) 208, 754, 1567, 1824, 2825, 2829, 3131
CaiI CAGNNNCTG 1 cut(s) 1697
CfoI GCGC 4 cut(s) 160, 822, 1762, 2607
Cfr10I RCCGGY 1 cut(s) 1748
Cfr13I GGNCC 7 cut(s) 895, 896, 1679, 1746, 2159, 2560, 2990
CseI GACGC 2 cut(s) 89, 327
Csp6I GTAC 7 cut(s) 717, 1318, 1578, 1631, 1655, 2867, 3092
CspAI ACCGGT 1 cut(s) 1748
CspCI CAANNNNNGTGG 4 cut(s) 592, 627, 1327, 1362
CviQI GTAC 7 cut(s) 717, 1318, 1578, 1631, 1655, 2867, 3092
DinI GGCGCC 2 cut(s) 159, 1761
DrdI GACNNNNNNGTC 1 cut(s) 2597
DseDI GACNNNNNNGTC 1 cut(s) 2597
EaeI YGGCCR 3 cut(s) 865, 2469, 2593
Eam1104I CTCTTC 4 cut(s) 1012, 2214, 2661, 3057
EarI CTCTTC 4 cut(s) 1012, 2214, 2661, 3057
EciI GGCGGA 1 cut(s) 405
Eco130I CCWWGG 5 cut(s) 1548, 1557, 1956, 2037, 2162
Eco24I GRGCYC 2 cut(s) 899, 985
Eco31I GGTCTC 2 cut(s) 1757, 2189
Eco47I GGWCC 5 cut(s) 1679, 1746, 2159, 2560, 2990
Eco57I CTGAAG 4 cut(s) 819, 1014, 2685, 3038
Eco88I CYCGRG 1 cut(s) 571
EcoNI CCTNNNNNAGG 1 cut(s) 1008
EcoRI GAATTC 1 cut(s) 2282
EcoT14I CCWWGG 5 cut(s) 1548, 1557, 1956, 2037, 2162
EcoT22I ATGCAT 2 cut(s) 766, 3169
EcoT38I GRGCYC 2 cut(s) 899, 985
EgeI GGCGCC 2 cut(s) 159, 1761
EheI GGCGCC 2 cut(s) 159, 1761
ErhI CCWWGG 5 cut(s) 1548, 1557, 1956, 2037, 2162
FaqI GGGAC 7 cut(s) 509, 798, 1759, 2019, 2031, 2172, 2645
FauI CCCGC 1 cut(s) 1735
FauNDI CATATG 2 cut(s) 1287, 2731
FbaI TGATCA 3 cut(s) 1156, 1195, 2701
FriOI GRGCYC 2 cut(s) 899, 985
FspBI CTAG 9 cut(s) 528, 929, 978, 987, 1050, 1413, 1566, 1688, 3011
GlaI GCGC 4 cut(s) 159, 821, 1761, 2606
GsaI CCCAGC 3 cut(s) 291, 2922, 3231
GsuI CTGGAG 5 cut(s) 333, 1116, 1739, 2049, 2891
HaeII RGCGCY 2 cut(s) 161, 1763
HaeIII GGCC 6 cut(s) 25, 867, 897, 2471, 2595, 2672
HapII CCGG 2 cut(s) 599, 1749
HgaI GACGC 2 cut(s) 89, 327
HhaI GCGC 4 cut(s) 160, 822, 1762, 2607
Hin1I GRCGYC 2 cut(s) 158, 1760
Hin6I GCGC 4 cut(s) 158, 820, 1760, 2605
HinP1I GCGC 4 cut(s) 158, 820, 1760, 2605
HincII GTYRAC 1 cut(s) 2942
HindII GTYRAC 1 cut(s) 2942
HindIII AAGCTT 1 cut(s) 2477
HpaII CCGG 2 cut(s) 599, 1749
HphI GGTGA 5 cut(s) 1151, 1190, 1914, 2539, 3257
Hpy166II GTNNAC 4 cut(s) 511, 2362, 2721, 2942
Hpy8I GTNNAC 4 cut(s) 511, 2362, 2721, 2942
Hpy99I CGWCG 2 cut(s) 105, 343
HpyCH4III ACNGT 3 cut(s) 169, 1302, 1938
HpyCH4IV ACGT 2 cut(s) 2693, 3087
HpySE526I ACGT 2 cut(s) 2693, 3087
Hsp92I GRCGYC 2 cut(s) 158, 1760
HspAI GCGC 4 cut(s) 158, 820, 1760, 2605
KasI GGCGCC 2 cut(s) 157, 1759
KpnI GGTACC 1 cut(s) 1634
Ksp22I TGATCA 3 cut(s) 1156, 1195, 2701
MaeI CTAG 9 cut(s) 528, 929, 978, 987, 1050, 1413, 1566, 1688, 3011
MaeII ACGT 2 cut(s) 2693, 3087
MaeIII GTNAC 9 cut(s) 38, 128, 824, 1415, 1920, 1932, 2183, 2615, 2710
MflI RGATCY 4 cut(s) 539, 851, 1099, 1354
MhlI GDGCHC 4 cut(s) 899, 985, 2442, 2766
MlsI TGGCCA 2 cut(s) 867, 2471
MluNI TGGCCA 2 cut(s) 867, 2471
Mly113I GGCGCC 2 cut(s) 158, 1760
MlyI GAGTC 5 cut(s) 715, 1100, 1741, 2314, 2947
MmeI TCCRAC 2 cut(s) 321, 3084
Mox20I TGGCCA 2 cut(s) 867, 2471
Mph1103I ATGCAT 2 cut(s) 766, 3169
MroXI GAANNNNTTC 2 cut(s) 696, 1645
MscI TGGCCA 2 cut(s) 867, 2471
MseI TTAA 9 cut(s) 567, 1065, 1086, 1259, 1449, 1523, 2103, 2226, 3150
MslI CAYNNNNRTG 4 cut(s) 2730, 2734, 2769, 3162
Msp20I TGGCCA 2 cut(s) 867, 2471
MspA1I CMGCKG 2 cut(s) 620, 1694
MspI CCGG 2 cut(s) 599, 1749
Mva1269I GAATGC 2 cut(s) 190, 3169
MvnI CGCG 1 cut(s) 3129
NarI GGCGCC 2 cut(s) 158, 1760
NcoI CCATGG 3 cut(s) 1557, 2037, 2162
NdeI CATATG 2 cut(s) 1287, 2731
NheI GCTAGC 1 cut(s) 1565
NmuCI GTSAC 6 cut(s) 128, 824, 1920, 1932, 2615, 2710
NsiI ATGCAT 2 cut(s) 766, 3169
NspI RCATGY 5 cut(s) 877, 2020, 2276, 2827, 2867
PaeI GCATGC 1 cut(s) 2827
PaeR7I CTCGAG 1 cut(s) 571
PciI ACATGT 1 cut(s) 2272
PcsI WCGNNNNNNNCGW 1 cut(s) 161
PctI GAATGC 2 cut(s) 190, 3169
PdmI GAANNNNTTC 2 cut(s) 696, 1645
Pfl23II CGTACG 1 cut(s) 716
PflFI GACNNNGTC 1 cut(s) 2716
PflMI CCANNNNNTGG 2 cut(s) 32, 892
PfoI TCCNGGA 3 cut(s) 310, 1636, 2026
PinAI ACCGGT 1 cut(s) 1748
PleI GAGTC 5 cut(s) 715, 1099, 1740, 2313, 2946
PluTI GGCGCC 2 cut(s) 161, 1763
PpsI GAGTC 5 cut(s) 715, 1099, 1740, 2313, 2946
PscI ACATGT 1 cut(s) 2272
PshBI ATTAAT 1 cut(s) 1086
Psp1406I AACGTT 1 cut(s) 2693
PspFI CCCAGC 3 cut(s) 287, 2918, 3227
PspLI CGTACG 1 cut(s) 716
PspOMI GGGCCC 1 cut(s) 895
PspPI GGNCC 7 cut(s) 895, 896, 1679, 1746, 2159, 2560, 2990
PspXI VCTCGAGB 1 cut(s) 571
PsrI GAACNNNNNNTAC 2 cut(s) 31, 63
PstI CTGCAG 4 cut(s) 883, 925, 1618, 1816
PstNI CAGNNNCTG 1 cut(s) 1697
PsuI RGATCY 4 cut(s) 539, 851, 1099, 1354
PsyI GACNNNGTC 1 cut(s) 2716
PvuII CAGCTG 1 cut(s) 1694
RsaI GTAC 7 cut(s) 718, 1319, 1579, 1632, 1656, 2868, 3093
RsaNI GTAC 7 cut(s) 717, 1318, 1578, 1631, 1655, 2867, 3092
RseI CAYNNNNRTG 4 cut(s) 2730, 2734, 2769, 3162
SaqAI TTAA 9 cut(s) 567, 1065, 1086, 1259, 1449, 1523, 2103, 2226, 3150
Sau96I GGNCC 7 cut(s) 895, 896, 1679, 1746, 2159, 2560, 2990
ScaI AGTACT 1 cut(s) 1656
SchI GAGTC 5 cut(s) 715, 1100, 1741, 2314, 2947
SduI GDGCHC 4 cut(s) 899, 985, 2442, 2766
SfcI CTRYAG 4 cut(s) 879, 921, 1614, 1812
SfoI GGCGCC 2 cut(s) 159, 1761
Sfr274I CTCGAG 1 cut(s) 571
SinI GGWCC 5 cut(s) 1679, 1746, 2159, 2560, 2990
SlaI CTCGAG 1 cut(s) 571
SmiMI CAYNNNNRTG 4 cut(s) 2730, 2734, 2769, 3162
SmlI CTYRAG 4 cut(s) 571, 1792, 2379, 2759
SmoI CTYRAG 4 cut(s) 571, 1792, 2379, 2759
SpeI ACTAGT 1 cut(s) 3010
SphI GCATGC 1 cut(s) 2827
SsiI CCGC 9 cut(s) 161, 188, 210, 273, 416, 557, 620, 1742, 2291
SspDI GGCGCC 2 cut(s) 157, 1759
SspI AATATT 1 cut(s) 139
SspMI CTAG 9 cut(s) 528, 929, 978, 987, 1050, 1413, 1566, 1688, 3011
StyI CCWWGG 5 cut(s) 1548, 1557, 1956, 2037, 2162
TaaI ACNGT 3 cut(s) 169, 1302, 1938
TaiI ACGT 2 cut(s) 2696, 3090
TaqI TCGA 5 cut(s) 389, 431, 551, 572, 1442
TatI WGTACW 3 cut(s) 1654, 2866, 3091
TauI GCSGC 2 cut(s) 163, 559
Tru1I TTAA 9 cut(s) 567, 1065, 1086, 1259, 1449, 1523, 2103, 2226, 3150
Tru9I TTAA 9 cut(s) 567, 1065, 1086, 1259, 1449, 1523, 2103, 2226, 3150
TscAI CASTG 2 cut(s) 2293, 2771
TseFI GTSAC 6 cut(s) 128, 824, 1920, 1932, 2615, 2710
Tsp45I GTSAC 6 cut(s) 128, 824, 1920, 1932, 2615, 2710
TspGWI ACGGA 1 cut(s) 2357
TspRI CASTG 2 cut(s) 2293, 2771
Tth111I GACNNNGTC 1 cut(s) 2716
Van91I CCANNNNNTGG 2 cut(s) 32, 892
VpaK11BI GGWCC 5 cut(s) 1679, 1746, 2159, 2560, 2990
VspI ATTAAT 1 cut(s) 1086
XagI CCTNNNNNAGG 1 cut(s) 1008
XapI RAATTY 7 cut(s) 62, 1126, 1498, 1879, 2131, 2137, 2282
XceI RCATGY 5 cut(s) 877, 2020, 2276, 2827, 2867
XcmI CCANNNNNNNNNTGG 1 cut(s) 1577
XhoI CTCGAG 1 cut(s) 571
XmnI GAANNNNTTC 2 cut(s) 696, 1645
XspI CTAG 9 cut(s) 528, 929, 978, 987, 1050, 1413, 1566, 1688, 3011
ZrmI AGTACT 1 cut(s) 1656
Zsp2I ATGCAT 2 cut(s) 766, 3169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.