MD03G1087000.v1.1

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002776
Physical Location & Seq
Reverse (-)
7089779 .. 7097555
7777 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1087000.v1.1.491

Sequence Viewer

Length: 3405 bp
ATGTTGTCGGAATTAGGAAGGAGGCCAATGCTCGGAGGAAACGAGGGTTCCTTTGGGGATGAATTCGAGAAGGAGATTGGGATGCTGCTGAGGGAGCAGCGTAGGCAAGACGCCGATGATCGCGAAAGTGAGCTGAATATATATAGAAGTGGATCAGCACCTCCCACTGTGGAGGGTTCTTTGAATGCGGTCGGTGGGTTGTTTGCTGGTGGCGGTGGCGGCGGTGGCGGTGGCGGTGCTGGTGCCGCCTTTTCGGAGTTTCCTGGAGCTAAGAATGGAAATGGGTTCGAGTCCGAAGAGGAGCTTAGGTCTGATCCTGCTTATATTCAATACTATTACTCGAATGTGAACCTGAATCCGCGGCTTCCGCCTCCTTTGCTATCGAAGGAGGATTGGAGGTTTGCACAAAGGATGAAAGGAGGAGGGAGTTCTGTGTTGGGTGGGATAGGAGACAGGAGGAAAGTGAATAGGGTTGACGAGGCCAGCGGAAGATCGCTGTTTTCGATGCCGCCGGGTTTCAACTCAAGGAAACAAGAGAGTGAGACCGAGTCGGACAAAGTGCGTGGCTCAGCAGAGTGGGGTGTTGATGGACTGATTGGTCTGCCAGGTTTAGGACTTGGTAACAAACAAAAGAGCCTTGCCGAAATCTTTCAGGATGACTTGGGTCGTGCTGCTCCTGTTTCTGGGCATCCTTCTCGTCCAGCAAGCAGGAATGCATTTGATGACAATGCAGAGTCTGTTGGTTCTGCTGAATCTGATCTAGCTCATTTGCGCCGTGACTTGATGACTTCTGATGCTCTACGATCTAGTGCAAATGGCCAGGGCTCATCTGCAGCACAAAGTATGGGCCCGCCTTCGTCATATTCGTATGCTGCAGCCCTAGGCGCTTCCTTATCAAGAAGCACCACTCCTGATCCTCAAGTTGTTGCTAGGGCTCCTAGTCCTTGCCTCACACCTATTGGAGGAGGACGGGTTGGTGCTTCAGAAAAGAGAGGCATTAGTAGTCCGAGTTCCTTCAATGGTGTCTCATCTGGCAGGAATGAGTCTGGGGATCTGGTGGGCGCGTTTTCCAGCATGAACTTGTCTGCAAATGGTGTAAAAGATGATGAGAGCCATTTGCCATCACAGATTAAGCAAGATGCAGATGATCACCAGAATTATCTCTTTGGTCTTCAAGGTGGCGAGAATCATGCCAGGCAGCTTGCATACCTCAAGAAGTCTGAATCTGGGCATATGCACATGCCTTCTGCTCCACACTCTGCAAAAGGTTCATACACAGATTTGGGTAAGAGCAATGGCGGTGGTTCAGATTCCTCTGATAGGCAAGTTGAACTGCAAAAGTCTGCTGTTTCTTCGGGTAACTTGTACTCGAAGGGATCTCCCACATCCAATCTTAATGGTGGGGGTGGCTTGCTTCATCAGTATCAGCAAGTGGATCATGCGAACTCACCATTTCCTAACTATGGTTTAAGTGGGTACTCTATGAATCCAGCGTTGGCGTCCATGGTGGCTAGCCAACTTGGCACTGGTAATCTTCCACCCTTGTTTGAATCAGCTATGGGATCCCCTGGAATGGATTCAAGAGCGCTTGGAGGCCGTATGTCCTCTGGACCAAACCTAGCAGCTGCTGCAAATGAATCACATAATCTTGGTGGCTTGGGGAGTCCGATTGCAGGGAGTGGTCTTCAGGCTCCCTTTGTTGATCCTATGTATCTTCAGTACTTGAGGACATCTGAGTATGCTGCAGCACAGCTTGCTGCTCTTAATGATCCTTCTGTGGATAGGAACTACTTGGGTAATTCATACATGAATTTACTGGAACTTCAAAAGGCGTATCTTGGAGCCTTGCTGTCACCACAGAAGTCACAGTATGGTGTTCCTATGGTTGGCAAGTCTGGTGGTTCTAATCATCAGGGGTATTATGGAAATCCTGCATTTGGTCTAGGCATGTCTTATCCTGGAAGTCCTCCTGTTATTCCTAACTCTCCAGTTGGACCAGGTACTCCTATTAGGCACAATGAACTCAATATGTGTTATCCCTCTGGGATGAGGAATTTAGCTCCCTGGCACCTGGATGGTGGATGTAACATAGATGAAAGTTTTGCTTCTTCACTCTTAGAAGAATTCAAGAGCAACAAAGCCAAGTCTTTTGAACTTTCAGAGATCGGTGGACATGTTGTGGAGTTCAGTGCGGATCAGTATGGGAGTCGTTTTATTCAACAAAAACTTGAAACTGCCACTACAGAAGAGAAAAACATGGTTTATCAAGAAATCATGCCTCAAGCTCTTGCTCTAATGACTGATGTGTTTGGTAATTATGTGATTCAAAAGTTTTTTGAGCATGGACTTCAACCTCAGAGAAGGGAATTGGCCAACAAGCTTTTCAGTCATGTACTGACACTTAGCCTTCAAATGTATGGTTGTCGGGTGATCCAGAAGGCAATAGAAGTTGTAGATCTGGACCAAAAGATCAAAATGGTTGAAGAACTTGACGGTCATGTCATGCGCTGCGTACGTGATCAGAATGGGAACCATGTCATCCAGAAGTGTATTGAATGTGTCCCTGAAGAGGCCATTCGTTTTATTGTGTCAACATTTTTTGATCAAGTTGTGACCCTTTCAACCCATCCATATGGGTGTCGTGTGATACAGAGAGTTCTTGAGCACTGCAAAGATGAGAATACACAGAGTAAAGTTATGGATGAGATTCTAGGAGCTGTTAGCATGTTGGCACAAGATCAGTATGGCAATTATGTCGTTCAGCATGTGCTGGAGCATGGAAAACCGCATGAGCGCTCTGCTATCATAAAAGAATTAGCTGGGAAAATAGTCCAAATGAGTCAGCAGAAGTTTGCCTCCAATGTAGTAGAGAAGTGTTTAACTTTTGGAGGTCCTGTTGAGCGTGAATTGCTAGTGAATGAGATGCTTGGCACTACTGATGAAAATGAGCCTCTTCAGGCAATGATGAAGGATCAATTTGCAAACTATGTTGTACAAAAAGTGTTGGAGACTTGTGATGACCAACAACGTGAACTGATTCTTTCCCGAATTAAAGTCCATTTGAATGCATTAAAGAAATACACTTATGGAAAGCATATTGTTGCTCGTGTAGAAAAACTTGTTGCTGCTGGGGGAGAGTTGCTCAGTCTGCTCAGCACCCTGCTTAGGCGGTGTAGGAAAATAAAGTTGTTTGTACAGCTAACTGAGGCTAGTGTGTGTTTACTCTCCCTTCGTTGTCGTCTCTCTCCCTCGTATCTAGAGCTACTACCGGCTCTGCCTATCTTTCCGAAGACAGGTACGAGGCGGTTTGGAAATAAAGTTGTTTCTTCTATGTACTGGAAAACTGTACATTGTGTAGTTTTAAAGCTTATACGTAGAGTCGAAGCTGGCTGTGGGTTCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1135

Amino Acids

122.47

Weight (kDa)

6.48

Isoelectric Point (pI)

50.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 311 - 720 5.8e-163 Nucleic acid binding protein NABP
PUF PF00806 725 - 759 1e-10 Pumilio-family RNA binding repeat
PUF PF00806 763 - 794 2.4e-07 Pumilio-family RNA binding repeat
PUF PF00806 797 - 830 1.9e-09 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 833 - 914 5.6e-08 NOP9-like PUF repeat domain
PUF PF00806 839 - 863 4.3e-06 Pumilio-family RNA binding repeat
PUF PF00806 869 - 904 9.1e-13 Pumilio-family RNA binding repeat
PUF PF00806 909 - 939 8.8e-10 Pumilio-family RNA binding repeat
PUF PF00806 943 - 976 6.2e-07 Pumilio-family RNA binding repeat
PUF PF00806 988 - 1017 2.1e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 597, 2498
AccB1I GGYRCC 2 cut(s) 242, 2067
AccII CGCG 3 cut(s) 123, 361, 1064
AcoI YGGCCR 2 cut(s) 817, 2370
AcsI RAATTY 4 cut(s) 62, 1810, 2053, 2123
AcuI CTGAAG 5 cut(s) 966, 1670, 1700, 2586, 2939
AcyI GRCGYC 2 cut(s) 111, 1499
AfeI AGCGCT 2 cut(s) 1587, 2795
AflIII ACRYGT 1 cut(s) 2173
AjnI CCWGG 9 cut(s) 262, 604, 819, 1193, 1567, 1957, 1996, 2063, 2070
AjuI GAANNNNNNNTTGG 4 cut(s) 1478, 1510, 2366, 2398
Alw21I GWGCWC 1 cut(s) 2667
Alw26I GTCTC 5 cut(s) 444, 536, 1030, 3002, 3245
AlwNI CAGNNNCTG 2 cut(s) 737, 1628
Aor51HI AGCGCT 2 cut(s) 1587, 2795
AoxI GGCC 7 cut(s) 23, 480, 817, 847, 1594, 2370, 2571
ApaI GGGCCC 1 cut(s) 851
ApoI RAATTY 4 cut(s) 62, 1810, 2053, 2123
Asp700I GAANNNNTTC 3 cut(s) 648, 1576, 3036
AspA2I CCTAGG 1 cut(s) 880
AspLEI GCGC 6 cut(s) 774, 887, 1064, 1588, 2508, 2796
AspS9I GGNCC 6 cut(s) 847, 848, 1610, 1994, 2461, 2891
AsuC2I CCSGG 1 cut(s) 513
AsuHPI GGTGA 4 cut(s) 1142, 1440, 1845, 2440
AsuNHI GCTAGC 1 cut(s) 1511
AvaII GGWCC 4 cut(s) 1610, 1994, 2461, 2891
AvrII CCTAGG 1 cut(s) 880
BaeGI GKGCMC 1 cut(s) 851
BalI TGGCCA 2 cut(s) 819, 2372
BamHI GGATCC 1 cut(s) 1562
BanI GGYRCC 2 cut(s) 242, 2067
BanII GRGCYC 3 cut(s) 827, 851, 937
BauI CACGAG 1 cut(s) 3105
BbsI GAAGAC 3 cut(s) 1163, 1676, 3296
Bbv12I GWGCWC 1 cut(s) 2667
BbvCI CCTCAGC 1 cut(s) 89
BccI CCATC 4 cut(s) 581, 1129, 2069, 2634
BceAI ACGGC 2 cut(s) 759, 1581
BcgI CGANNNNNNTGC 6 cut(s) 677, 711, 1172, 1206, 2737, 2771
BciT130I CCWGG 9 cut(s) 264, 606, 821, 1195, 1569, 1959, 1998, 2065, 2072
BclI TGATCA 3 cut(s) 1147, 2518, 2602
BcnI CCSGG 1 cut(s) 513
BcoDI GTCTC 5 cut(s) 444, 536, 1030, 3002, 3245
BfmI CTRYAG 4 cut(s) 831, 873, 1743, 2241
BfoI RGCGCY 3 cut(s) 888, 1589, 2797
BglI GCCNNNNNGGC 1 cut(s) 1521
BglII AGATCT 1 cut(s) 2455
BlnI CCTAGG 1 cut(s) 880
BlpI GCTNAGC 2 cut(s) 568, 3152
BmcAI AGTACT 1 cut(s) 1721
Bme18I GGWCC 4 cut(s) 1610, 1994, 2461, 2891
BmgT120I GGNCC 6 cut(s) 847, 848, 1610, 1994, 2461, 2891
BmiI GGNNCC 9 cut(s) 49, 244, 849, 936, 1564, 1692, 1843, 2069, 2531
BmsI GCATC 6 cut(s) 72, 495, 697, 784, 1129, 2913
BmtI GCTAGC 1 cut(s) 1515
BoxI GACNNNNGTC 1 cut(s) 663
BpiI GAAGAC 3 cut(s) 1163, 1676, 3296
BplI GAGNNNNNCTC 2 cut(s) 3126, 3158
BpmI CTGGAG 3 cut(s) 285, 1971, 2792
Bpu10I CCTNAGC 3 cut(s) 89, 305, 3164
Bpu1102I GCTNAGC 2 cut(s) 568, 3152
BpuEI CTTGAG 6 cut(s) 508, 903, 1196, 1744, 2265, 2681
BpuMI CCSGG 1 cut(s) 513
BsaAI YACGTR 2 cut(s) 2516, 3374
BsaHI GRCGYC 2 cut(s) 111, 1499
BsaI GGTCTC 1 cut(s) 536
BsaJI CCNNGG 6 cut(s) 359, 820, 880, 1503, 1567, 2063
BsaXI ACNNNNNCTCC 6 cut(s) 293, 323, 957, 987, 2880, 2910
Bse118I RCCGGY 1 cut(s) 3268
Bse1I ACTGG 4 cut(s) 1531, 1821, 1988, 3341
Bse3DI GCAATG 2 cut(s) 1300, 2967
BseBI CCWGG 9 cut(s) 264, 606, 821, 1195, 1569, 1959, 1998, 2065, 2072
BseDI CCNNGG 6 cut(s) 359, 820, 880, 1503, 1567, 2063
BseMI GCAATG 2 cut(s) 1300, 2967
BseMII CTCAG 7 cut(s) 80, 582, 1725, 2369, 3157, 3166, 3195
BseNI ACTGG 4 cut(s) 1531, 1821, 1988, 3341
BseRI GAGGAG 3 cut(s) 314, 435, 978
BseSI GKGCMC 1 cut(s) 851
BseYI CCCAGC 2 cut(s) 2819, 3128
Bsh1236I CGCG 3 cut(s) 123, 361, 1064
Bsh1285I CGRYCG 1 cut(s) 192
BshFI GGCC 7 cut(s) 25, 482, 819, 849, 1596, 2372, 2573
BshNI GGYRCC 2 cut(s) 242, 2067
BsiEI CGRYCG 1 cut(s) 192
BsiHKAI GWGCWC 1 cut(s) 2667
BsiSI CCGG 2 cut(s) 512, 3269
BsiWI CGTACG 1 cut(s) 2512
BslFI GGGAC 1 cut(s) 2546
BsmAI GTCTC 5 cut(s) 444, 536, 1030, 3002, 3245
BsmBI CGTCTC 1 cut(s) 3245
BsmFI GGGAC 1 cut(s) 2546
BsmI GAATGC 3 cut(s) 190, 718, 3070
BsnI GGCC 7 cut(s) 25, 482, 819, 849, 1596, 2372, 2573
Bso31I GGTCTC 1 cut(s) 536
Bsp120I GGGCCC 1 cut(s) 847
Bsp1286I GDGCHC 4 cut(s) 827, 851, 937, 2667
Bsp1407I TGTACA 3 cut(s) 2992, 3193, 3346
Bsp1720I GCTNAGC 2 cut(s) 568, 3152
Bsp19I CCATGG 1 cut(s) 1503
Bsp68I TCGCGA 1 cut(s) 123
BspANI GGCC 7 cut(s) 25, 482, 819, 849, 1596, 2372, 2573
BspCNI CTCAG 7 cut(s) 81, 581, 1726, 2368, 3156, 3165, 3196
BspFNI CGCG 3 cut(s) 123, 361, 1064
BspLI GGNNCC 9 cut(s) 49, 244, 849, 936, 1564, 1692, 1843, 2069, 2531
BspMAI CTGCAG 3 cut(s) 835, 877, 1747
BspOI GCTAGC 1 cut(s) 1515
BspT107I GGYRCC 2 cut(s) 242, 2067
BspTNI GGTCTC 1 cut(s) 536
BsrDI GCAATG 2 cut(s) 1300, 2967
BsrFI RCCGGY 1 cut(s) 3268
BsrGI TGTACA 3 cut(s) 2992, 3193, 3346
BsrI ACTGG 4 cut(s) 1531, 1821, 1988, 3341
BssAI RCCGGY 1 cut(s) 3268
BssECI CCNNGG 6 cut(s) 359, 820, 880, 1503, 1567, 2063
BssNI GRCGYC 2 cut(s) 111, 1499
BssSI CACGAG 1 cut(s) 3105
BssT1I CCWWGG 2 cut(s) 880, 1503
Bst2BI CACGAG 1 cut(s) 3105
Bst2UI CCWGG 9 cut(s) 264, 606, 821, 1195, 1569, 1959, 1998, 2065, 2072
Bst4CI ACNGT 4 cut(s) 169, 1869, 2495, 3346
Bst6I CTCTTC 4 cut(s) 291, 2241, 2562, 2958
BstACI GRCGYC 2 cut(s) 111, 1499
BstAPI GCANNNNNTGC 2 cut(s) 1628, 1754
BstAUI TGTACA 3 cut(s) 2992, 3193, 3346
BstBAI YACGTR 2 cut(s) 2516, 3374
BstC8I GCNNGC 8 cut(s) 484, 706, 851, 1203, 1412, 1513, 1755, 3388
BstDSI CCRYGG 2 cut(s) 359, 1503
BstENI CCTNNNNNAGG 2 cut(s) 960, 1318
BstFNI CGCG 3 cut(s) 123, 361, 1064
BstH2I RGCGCY 3 cut(s) 888, 1589, 2797
BstHHI GCGC 6 cut(s) 774, 887, 1064, 1588, 2508, 2796
BstMAI GTCTC 5 cut(s) 444, 536, 1030, 3002, 3245
BstMCI CGRYCG 1 cut(s) 192
BstNI CCWGG 9 cut(s) 264, 606, 821, 1195, 1569, 1959, 1998, 2065, 2072
BstNSI RCATGY 5 cut(s) 1243, 1951, 2177, 2728, 2768
BstPAI GACNNNNGTC 1 cut(s) 663
BstSFI CTRYAG 4 cut(s) 831, 873, 1743, 2241
BstSLI GKGCMC 1 cut(s) 851
BstSNI TACGTA 1 cut(s) 3374
BstUI CGCG 3 cut(s) 123, 361, 1064
BstV2I GAAGAC 3 cut(s) 1163, 1676, 3296
BstX2I RGATCY 4 cut(s) 1051, 1376, 1562, 2455
BstXI CCANNNNNNTGG 1 cut(s) 2633
BstYI RGATCY 4 cut(s) 1051, 1376, 1562, 2455
BsuRI GGCC 7 cut(s) 25, 482, 819, 849, 1596, 2372, 2573
BtgI CCRYGG 2 cut(s) 359, 1503
BtsI GCAGTG 1 cut(s) 2665
BtsIMutI CAGTG 4 cut(s) 165, 1524, 2194, 2665
BtuMI TCGCGA 1 cut(s) 123
Cac8I GCNNGC 8 cut(s) 484, 706, 851, 1203, 1412, 1513, 1755, 3388
CaiI CAGNNNCTG 2 cut(s) 737, 1628
CfoI GCGC 6 cut(s) 774, 887, 1064, 1588, 2508, 2796
Cfr10I RCCGGY 1 cut(s) 3268
Cfr13I GGNCC 6 cut(s) 847, 848, 1610, 1994, 2461, 2891
Cfr42I CCGCGG 1 cut(s) 362
CseI GACGC 2 cut(s) 119, 1488
CsiI ACCWGGT 1 cut(s) 1996
CspCI CAANNNNNGTGG 6 cut(s) 544, 579, 1282, 1317, 1879, 1914
DraI TTTAAA 1 cut(s) 3363
DrdI GACNNNNNNGTC 2 cut(s) 597, 2498
DseDI GACNNNNNNGTC 2 cut(s) 597, 2498
EaeI YGGCCR 2 cut(s) 817, 2370
Eam1104I CTCTTC 4 cut(s) 291, 2241, 2562, 2958
EarI CTCTTC 4 cut(s) 291, 2241, 2562, 2958
EciI GGCGGA 1 cut(s) 357
Eco105I TACGTA 1 cut(s) 3374
Eco130I CCWWGG 2 cut(s) 880, 1503
Eco24I GRGCYC 3 cut(s) 827, 851, 937
Eco31I GGTCTC 1 cut(s) 536
Eco47I GGWCC 4 cut(s) 1610, 1994, 2461, 2891
Eco47III AGCGCT 2 cut(s) 1587, 2795
Eco57I CTGAAG 5 cut(s) 966, 1670, 1700, 2586, 2939
EcoNI CCTNNNNNAGG 2 cut(s) 960, 1318
EcoO109I RGGNCCY 1 cut(s) 2891
EcoRI GAATTC 2 cut(s) 62, 2123
EcoRII CCWGG 9 cut(s) 262, 604, 819, 1193, 1567, 1957, 1996, 2063, 2070
EcoT14I CCWWGG 2 cut(s) 880, 1503
EcoT22I ATGCAT 2 cut(s) 718, 3070
EcoT38I GRGCYC 3 cut(s) 827, 851, 937
ErhI CCWWGG 2 cut(s) 880, 1503
Esp3I CGTCTC 1 cut(s) 3245
FalI AAGNNNNNCTT 4 cut(s) 288, 320, 2089, 2121
FaqI GGGAC 1 cut(s) 2546
FauI CCCGC 1 cut(s) 858
FauNDI CATATG 2 cut(s) 1233, 2632
FbaI TGATCA 3 cut(s) 1147, 2518, 2602
FriOI GRGCYC 3 cut(s) 827, 851, 937
GlaI GCGC 6 cut(s) 773, 886, 1063, 1587, 2507, 2795
GsaI CCCAGC 2 cut(s) 2823, 3132
GsuI CTGGAG 3 cut(s) 285, 1971, 2792
HaeII RGCGCY 3 cut(s) 888, 1589, 2797
HaeIII GGCC 7 cut(s) 25, 482, 819, 849, 1596, 2372, 2573
HapII CCGG 2 cut(s) 512, 3269
HgaI GACGC 2 cut(s) 119, 1488
HhaI GCGC 6 cut(s) 774, 887, 1064, 1588, 2508, 2796
Hin1I GRCGYC 2 cut(s) 111, 1499
Hin6I GCGC 6 cut(s) 772, 885, 1062, 1586, 2506, 2794
HinP1I GCGC 6 cut(s) 772, 885, 1062, 1586, 2506, 2794
HincII GTYRAC 2 cut(s) 475, 2592
HindII GTYRAC 2 cut(s) 475, 2592
HindIII AAGCTT 2 cut(s) 2378, 3365
HpaII CCGG 2 cut(s) 512, 3269
HphI GGTGA 4 cut(s) 1142, 1440, 1845, 2440
Hpy166II GTNNAC 6 cut(s) 349, 475, 2171, 2592, 3032, 3221
Hpy8I GTNNAC 6 cut(s) 349, 475, 2171, 2592, 3032, 3221
HpyCH4III ACNGT 4 cut(s) 169, 1869, 2495, 3346
HpyCH4IV ACGT 3 cut(s) 2515, 3028, 3373
HpySE526I ACGT 3 cut(s) 2515, 3028, 3373
Hsp92I GRCGYC 2 cut(s) 111, 1499
HspAI GCGC 6 cut(s) 772, 885, 1062, 1586, 2506, 2794
Ksp22I TGATCA 3 cut(s) 1147, 2518, 2602
KspI CCGCGG 1 cut(s) 362
LweI GCATC 6 cut(s) 72, 495, 697, 784, 1129, 2913
MabI ACCWGGT 1 cut(s) 1996
MaeII ACGT 3 cut(s) 2515, 3028, 3373
MaeIII GTNAC 7 cut(s) 620, 776, 1358, 1851, 1863, 2084, 2611
MflI RGATCY 4 cut(s) 1051, 1376, 1562, 2455
MhlI GDGCHC 4 cut(s) 827, 851, 937, 2667
MlsI TGGCCA 2 cut(s) 819, 2372
MluNI TGGCCA 2 cut(s) 819, 2372
MlyI GAGTC 8 cut(s) 299, 557, 743, 1052, 1672, 2215, 2848, 3387
MmeI TCCRAC 3 cut(s) 531, 1972, 2985
Mox20I TGGCCA 2 cut(s) 819, 2372
Mph1103I ATGCAT 2 cut(s) 718, 3070
MroXI GAANNNNTTC 3 cut(s) 648, 1576, 3036
MscI TGGCCA 2 cut(s) 819, 2372
MseI TTAA 8 cut(s) 1131, 1395, 1469, 1764, 2879, 3051, 3071, 3362
MslI CAYNNNNRTG 4 cut(s) 2073, 2631, 2635, 3063
Msp20I TGGCCA 2 cut(s) 819, 2372
MspA1I CMGCKG 3 cut(s) 361, 486, 1625
MspI CCGG 2 cut(s) 512, 3269
Mva1269I GAATGC 3 cut(s) 190, 718, 3070
MvaI CCWGG 9 cut(s) 264, 606, 821, 1195, 1569, 1959, 1998, 2065, 2072
MvnI CGCG 3 cut(s) 123, 361, 1064
NciI CCSGG 1 cut(s) 513
NcoI CCATGG 1 cut(s) 1503
NdeI CATATG 2 cut(s) 1233, 2632
NheI GCTAGC 1 cut(s) 1511
NlaIV GGNNCC 9 cut(s) 49, 244, 849, 936, 1564, 1692, 1843, 2069, 2531
NmuCI GTSAC 4 cut(s) 776, 1851, 1863, 2611
NruI TCGCGA 1 cut(s) 123
NsiI ATGCAT 2 cut(s) 718, 3070
NspI RCATGY 5 cut(s) 1243, 1951, 2177, 2728, 2768
PciI ACATGT 1 cut(s) 2173
PcsI WCGNNNNNNNCGW 3 cut(s) 39, 500, 863
PctI GAATGC 3 cut(s) 190, 718, 3070
PdmI GAANNNNTTC 3 cut(s) 648, 1576, 3036
Pfl23II CGTACG 1 cut(s) 2512
PflFI GACNNNGTC 1 cut(s) 547
PfoI TCCNGGA 2 cut(s) 262, 1957
PleI GAGTC 8 cut(s) 298, 556, 742, 1051, 1671, 2214, 2847, 3386
PpsI GAGTC 8 cut(s) 298, 556, 742, 1051, 1671, 2214, 2847, 3386
Ppu21I YACGTR 2 cut(s) 2516, 3374
PpuMI RGGWCCY 1 cut(s) 2891
PscI ACATGT 1 cut(s) 2173
PshAI GACNNNNGTC 1 cut(s) 663
Psp5II RGGWCCY 1 cut(s) 2891
Psp6I CCWGG 9 cut(s) 262, 604, 819, 1193, 1567, 1957, 1996, 2063, 2070
PspFI CCCAGC 2 cut(s) 2819, 3128
PspGI CCWGG 9 cut(s) 262, 604, 819, 1193, 1567, 1957, 1996, 2063, 2070
PspLI CGTACG 1 cut(s) 2512
PspN4I GGNNCC 9 cut(s) 49, 244, 849, 936, 1564, 1692, 1843, 2069, 2531
PspOMI GGGCCC 1 cut(s) 847
PspPI GGNCC 6 cut(s) 847, 848, 1610, 1994, 2461, 2891
PspPPI RGGWCCY 1 cut(s) 2891
PsrI GAACNNNNNNTAC 2 cut(s) 994, 1026
PstI CTGCAG 3 cut(s) 835, 877, 1747
PstNI CAGNNNCTG 2 cut(s) 737, 1628
PsuI RGATCY 4 cut(s) 1051, 1376, 1562, 2455
PsyI GACNNNGTC 1 cut(s) 547
PvuII CAGCTG 1 cut(s) 1625
RruI TCGCGA 1 cut(s) 123
RseI CAYNNNNRTG 4 cut(s) 2073, 2631, 2635, 3063
SacII CCGCGG 1 cut(s) 362
SaqAI TTAA 8 cut(s) 1131, 1395, 1469, 1764, 2879, 3051, 3071, 3362
Sau96I GGNCC 6 cut(s) 847, 848, 1610, 1994, 2461, 2891
ScaI AGTACT 1 cut(s) 1721
SchI GAGTC 8 cut(s) 299, 557, 743, 1052, 1672, 2215, 2848, 3387
SduI GDGCHC 4 cut(s) 827, 851, 937, 2667
SexAI ACCWGGT 1 cut(s) 1996
SfaNI GCATC 6 cut(s) 72, 495, 697, 784, 1129, 2913
SfcI CTRYAG 4 cut(s) 831, 873, 1743, 2241
Sfr303I CCGCGG 1 cut(s) 362
SgrBI CCGCGG 1 cut(s) 362
SinI GGWCC 4 cut(s) 1610, 1994, 2461, 2891
SmiMI CAYNNNNRTG 4 cut(s) 2073, 2631, 2635, 3063
SmlI CTYRAG 6 cut(s) 523, 918, 1211, 1723, 2280, 2660
SmoI CTYRAG 6 cut(s) 523, 918, 1211, 1723, 2280, 2660
SnaBI TACGTA 1 cut(s) 3374
StyI CCWWGG 2 cut(s) 880, 1503
TaaI ACNGT 4 cut(s) 169, 1869, 2495, 3346
TaiI ACGT 3 cut(s) 2518, 3031, 3376
TaqI TCGA 7 cut(s) 66, 288, 341, 383, 503, 1370, 3381
TaqII GACCGA 1 cut(s) 560
TatI WGTACW 7 cut(s) 1365, 1719, 2392, 2992, 3193, 3333, 3346
TauI GCSGC 4 cut(s) 222, 248, 364, 511
Tru1I TTAA 8 cut(s) 1131, 1395, 1469, 1764, 2879, 3051, 3071, 3362
Tru9I TTAA 8 cut(s) 1131, 1395, 1469, 1764, 2879, 3051, 3071, 3362
TscAI CASTG 4 cut(s) 172, 1531, 2194, 2672
TseFI GTSAC 4 cut(s) 776, 1851, 1863, 2611
Tsp45I GTSAC 4 cut(s) 776, 1851, 1863, 2611
TspRI CASTG 4 cut(s) 172, 1531, 2194, 2672
Tth111I GACNNNGTC 1 cut(s) 547
VpaK11BI GGWCC 4 cut(s) 1610, 1994, 2461, 2891
XagI CCTNNNNNAGG 2 cut(s) 960, 1318
XapI RAATTY 4 cut(s) 62, 1810, 2053, 2123
XbaI TCTAGA 1 cut(s) 3256
XceI RCATGY 5 cut(s) 1243, 1951, 2177, 2728, 2768
XcmI CCANNNNNNNNNTGG 1 cut(s) 1523
XmaJI CCTAGG 1 cut(s) 880
XmnI GAANNNNTTC 3 cut(s) 648, 1576, 3036
ZrmI AGTACT 1 cut(s) 1721
Zsp2I ATGCAT 2 cut(s) 718, 3070
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.