RLG00000027244

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002776
Physical Location & Seq
Reverse (-)
9902467 .. 9908888
6422 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027244

Sequence Viewer

Length: 3066 bp
ATGATGTCCGAGATGTCAATGCGATCCATGCTTAAGAACAACGGCGATTACAGTGAAGATTTGAGCCTTCTGATTCGTCAGCAGAGGCAAGAAGTGAGTGAGCGAGAGAAGGAGCTCAATCCATATAGAAGTGGGTCGGCCCCACCAACCGTAGAAGGGTCGCTGAACGCCGTCGGCGGTCTTTTTGACGCCGTTAACGGCTTTGCGAGCGAGGAGGAGCTAAGGGCTGATCCGGCTTATCATAGCTACTACTACGCCAATGTCAATCTCAATCCGCGGCTACCGCCGCCGCTGAGGTCCAAGGAGGACTGGCGGTTTGCGCAGCGGGGCGGCGCCGGAGGAGGTTCTGGGGTCGGAGGCATTGGGGATAGGAGGAAAGGCGGGAGGAGTGGTGGTGGTGAAGGGTCTCTTTTTTCGGTGCAGCCGGAGAATGGGGCGGCGGCGAGGAATGGAGAGTGGGGCGGAGATGGGCTGATTGGGTTGCCCGGGTTGGGCCTTGGGAGTAGACAGAAGAGTATTGCCGAGATTCTTCAGGATGATATACAGAATACAACTGGCTCAAGGCACCCTTCTCGTCCAGCTAGCCGGAATGCATTTGATGATGGAGTTGAAACTTCAGATACCCAGTATGCTCAGATGCATCGTGATTTGGCATCTTTAGATGCTTTGCGCTCTGGTGGAAATAAGCAGGGTATGTCTGCTGCCCAGAACTTTGGTTCCTCGGGCTCTCATACTTATGCTTCTGCTCTAGGTGGTTCACTGTCAAGAAGCACCACACCAGATCCTCAGCTTGTATCTAGGGCTCCTAGTCCCCGGATTCCACCAGTTGGAGGAGGAAGAGCTCCTTCCACTGATAAAAATAGTGTCGGTGGTCAAAATTCATATAATGGTATCGCATCCAATGTGAAAGAGTCTGCAGATCTGGTAGCTGCCTTATCTGGTATGAACCTGTCAAAAAACAGCAGGATGCATGAAGAGAATCATGCACATTCACAGATTCAAGGTGATAACCACTTTGATATGCAAGGGGATCGGAACCATATTAAACAAAATTCATATATGAACAAGCCTGATTCAGGGAATTATCATTTGCATTCTGTTTCCCAGTCTACCAAAAACTCTTATCAGAATATGGCCAAAGGCAGTGGATTTGGGAGGGACCAGAACAACCCTTCACTGATGTCTGATGGGCTAGCTGAAATTAACAATCCTGCTGTATCTTCTGCTAACTCATACTTGAGAGGACCCTCTGCCCCAGCTCTTAATGGCCGAGGAAGCTCACTCTCTCACTATCAGAATGTAGACGGTATGAATTCCTCATTTGCAAACTATGGGTTAGCTGGGTATTCTGTTAGTCCATCGTCACCCTCCATGATGGGGAGCCCTCTGGGTAATGGTAATTTACCACCCTTGTTTGAAAATGCTGCTGCTGTTTCTGCAATGGGTGGCTTGGACTCTGGAGCCTTTGGAGGAGGTATGTCTCTGGGACCAAATTTGTTAGCAGCAGCTGCTGAATTGCAGAATATGGGGAGGGGTGGCAATCACACTGCCGGAGGTGCTCTTCAGATGCCCCTGATGGACCCCTTGTATCTGCAGTATTTAAGATCAAATGAGTATGCTGCTGCACAGCTTGCATCCCTTCATGACCCCACAGCAGACAGGGAAGGCATGTATATGGACTTTCTGGGGTTGCAAAAAGCTTATCTGGGACAATTACTTTCGCCTCAAAAATCACAGTTTGGTGCTCCCTACATGGGTAAGTCTGGTAGCTTGAATCATGGTTATTATGGAAATCCAGCATTTGGGCTTGGTATGTCTTATTCTGGAAGTCCCCTTCTTCCAAACTCTCCCGTTGGACCAGGTAGTCCTGTCAGGCACAGTGACAGGAACTTGCGTTTTTCTTCGGGGATGAGGAACATGAGTGGAGGTCTCATGGGAGCTTGGCACTCTGAAACTGGTGGAAATTTCGATGAAAGTTTTGCATCCTCTTTGCTAGATGAGTTCAAGAGCAATAAGACTAAATGTTTTGAACTTTCAGAAATTTCAGGCCATGTTGTTGAGTTCAGTGCGGACCAGTATGGGAGTAGGTTCATTCAACAGAAACTTGAAACTGCCACAACCGAAGAAAAGAACATGGTTTTTGATGAAATTATGCCCCAAGCCCTTTCTTTGATGACTGATGTGTTTGGTAATTATGTGATTCAGAAGTTTTTCGAGCACGGTTCTGCAGCACAAATTAGAGAACTGGCTGACCAGCTCACTGGGCATGTGCTGACCCTTAGCCTTCAAATGTATGGCTGTCGAGTGATCCAGAAGGCTATAGAAGTTGTTGATCTGGACCAGCAGACTCAAATGGTCACAGAACTGGATGGTCACATTATGCGTTGTGTTCGTGATCAAAATGGGAACCATGTCATCCAGAAGTGTATTGAATGTATACCTGAAGATGCTATCCAGTTTGTTGTTTCAACCTTTTATGATCAAGTTGTGACACTGTCAACTCATCCATATGGTTGTCGTGTCATACAGAGAATTTTGGAGCACTGCCATGATCCCAAAACGCAGCAGATAATGATGGATGAGATTTTGCATGCTGTTTGCACTTTGGCACAAGATCAGTATGGAAATTATGTTGTGCAGCATGTGCTGGAGCATGGAAAGCCAGACGAGCGCTCAGATATAATTAGGAAACTAACTGGGCAGATTGTTCAAATGAGCCAGCAGAAATTTGCCTCCAATGTTATAGAAAAGTGTTTAACTTTTGGAACTCTTGCTGAGCGCCAGGCCCTTGTAACTGAGATGCTCGGTACCACTGATGAAAACGAACCCCTTCAGGCAATGATGAAGGACCAGTTCGCAAATTATGTTGTACAGAAAGTTTTGGAAACTTGTGATGACCAGCAACTTGAACTAATCCTTAACCGTATTAAGGTTCATCTGAATGCTTTGAAGAAGTATACTTATGGGAAGCATATAGTTGCACGAGTAGAGAAACTTGTTGCTGCTGGAGAGAAGAGGATTAGCATCCTGACTCCACAATGTCCACCTGCTACTGCTACTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1022

Amino Acids

110.34

Weight (kDa)

5.94

Isoelectric Point (pI)

49.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 267 - 679 4.6e-150 Nucleic acid binding protein NABP
PUF PF00806 684 - 718 8e-12 Pumilio-family RNA binding repeat
PUF PF00806 721 - 753 4.7e-08 Pumilio-family RNA binding repeat
PUF PF00806 756 - 789 1.9e-09 Pumilio-family RNA binding repeat
PUF PF00806 828 - 863 2.3e-13 Pumilio-family RNA binding repeat
PUF PF00806 866 - 898 1.4e-09 Pumilio-family RNA binding repeat
PUF PF00806 901 - 934 2.1e-07 Pumilio-family RNA binding repeat
PUF PF00806 947 - 976 9.3e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3055
AasI GACNNNNNNGTC 1 cut(s) 1863
Acc16I TGCGCA 1 cut(s) 321
Acc36I ACCTGC 1 cut(s) 3055
Acc65I GGTACC 1 cut(s) 2807
AccB1I GGYRCC 3 cut(s) 332, 564, 2807
AccB7I CCANNNNNTGG 2 cut(s) 827, 1802
AccI GTMKAC 5 cut(s) 505, 1109, 1302, 2437, 2957
AccII CGCG 1 cut(s) 277
AclWI GGATC 6 cut(s) 18, 224, 776, 1038, 2302, 2546
AcoI YGGCCR 2 cut(s) 1134, 1267
AcsI RAATTY 8 cut(s) 877, 1051, 1312, 1492, 1963, 2040, 2532, 2726
AcuI CTGAAG 5 cut(s) 515, 600, 1547, 2463, 2816
AcyI GRCGYC 2 cut(s) 189, 333
AfaI GTAC 2 cut(s) 2809, 2871
AfeI AGCGCT 1 cut(s) 2672
AflII CTTAAG 1 cut(s) 32
AjnI CCWGG 2 cut(s) 1858, 2781
AloI GAACNNNNNNTCC 2 cut(s) 701, 733
Alw21I GWGCWC 6 cut(s) 117, 844, 1561, 1747, 2220, 2544
Alw26I GTCTC 3 cut(s) 411, 1485, 1934
AlwI GGATC 6 cut(s) 18, 224, 776, 1038, 2302, 2546
AlwNI CAGNNNCTG 2 cut(s) 1508, 1511
Ama87I CYCGRG 2 cut(s) 485, 721
Aor51HI AGCGCT 1 cut(s) 2672
AoxI GGCC 6 cut(s) 138, 493, 1134, 1267, 2047, 2784
ApoI RAATTY 8 cut(s) 877, 1051, 1312, 1492, 1963, 2040, 2532, 2726
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
Asp700I GAANNNNTTC 2 cut(s) 2210, 2829
Asp718I GGTACC 1 cut(s) 2807
AspLEI GCGC 5 cut(s) 322, 335, 672, 2673, 2781
AsuC2I CCSGG 3 cut(s) 486, 487, 814
AsuHPI GGTGA 3 cut(s) 410, 1016, 1356
AsuNHI GCTAGC 2 cut(s) 581, 1192
AvaI CYCGRG 2 cut(s) 485, 721
AvaII GGWCC 9 cut(s) 297, 1159, 1244, 1487, 1579, 1856, 2071, 2338, 2848
BalI TGGCCA 1 cut(s) 1136
BanI GGYRCC 3 cut(s) 332, 564, 2807
BanII GRGCYC 5 cut(s) 117, 728, 805, 844, 1385
BauI CACGAG 1 cut(s) 2982
Bbv12I GWGCWC 6 cut(s) 117, 844, 1561, 1747, 2220, 2544
BbvCI CCTCAGC 2 cut(s) 293, 786
BccI CCATC 8 cut(s) 461, 596, 1181, 1366, 1369, 1570, 2363, 2569
BceAI ACGGC 4 cut(s) 58, 155, 176, 214
BcgI CGANNNNNNTGC 4 cut(s) 554, 588, 1013, 1047
BciT130I CCWGG 2 cut(s) 1860, 2783
BclI TGATCA 2 cut(s) 2395, 2479
BcnI CCSGG 3 cut(s) 486, 487, 814
BcoDI GTCTC 3 cut(s) 411, 1485, 1934
BfaI CTAG 6 cut(s) 582, 749, 798, 807, 1193, 1994
BfmI CTRYAG 4 cut(s) 915, 1592, 2226, 2319
BfoI RGCGCY 3 cut(s) 336, 2674, 2782
BfrI CTTAAG 1 cut(s) 32
BfuAI ACCTGC 1 cut(s) 3055
BglII AGATCT 1 cut(s) 919
BlpI GCTNAGC 1 cut(s) 2775
Bme1390I CCNGG 5 cut(s) 486, 487, 814, 1860, 2783
Bme18I GGWCC 9 cut(s) 297, 1159, 1244, 1487, 1579, 1856, 2071, 2338, 2848
BmeT110I CYCGRG 2 cut(s) 485, 721
BmrFI CCNGG 5 cut(s) 486, 487, 814, 1860, 2783
BmrI ACTGGG 4 cut(s) 619, 1099, 2271, 2706
BmtI GCTAGC 2 cut(s) 585, 1196
BmuI ACTGGG 4 cut(s) 619, 1099, 2271, 2706
BpmI CTGGAG 3 cut(s) 1479, 2669, 3027
Bpu10I CCTNAGC 4 cut(s) 221, 293, 786, 2279
Bpu1102I GCTNAGC 1 cut(s) 2775
BpuEI CTTGAG 2 cut(s) 544, 1258
BpuMI CCSGG 3 cut(s) 486, 487, 814
BsaBI GATNNNNATC 1 cut(s) 3023
BsaHI GRCGYC 2 cut(s) 189, 333
BsaI GGTCTC 2 cut(s) 411, 1934
BsaJI CCNNGG 7 cut(s) 275, 300, 485, 496, 720, 812, 1270
BsaXI ACNNNNNCTCC 4 cut(s) 376, 379, 406, 409
Bse3DI GCAATG 2 cut(s) 1446, 2844
Bse8I GATNNNNATC 1 cut(s) 3023
BseBI CCWGG 2 cut(s) 1860, 2783
BseDI CCNNGG 7 cut(s) 275, 300, 485, 496, 720, 812, 1270
BseJI GATNNNNATC 1 cut(s) 3023
BseMI GCAATG 2 cut(s) 1446, 2844
BseMII CTCAG 6 cut(s) 284, 647, 800, 2688, 2766, 2787
BseRI GAGGAG 6 cut(s) 227, 230, 354, 400, 846, 1485
BseYI CCCAGC 2 cut(s) 1255, 1340
BsgI GTGCAG 3 cut(s) 440, 1608, 2657
Bsh1236I CGCG 1 cut(s) 277
BshFI GGCC 6 cut(s) 140, 495, 1136, 1269, 2049, 2786
BshNI GGYRCC 3 cut(s) 332, 564, 2807
BsiHKAI GWGCWC 6 cut(s) 117, 844, 1561, 1747, 2220, 2544
BsiHKCI CYCGRG 2 cut(s) 485, 721
BsiSI CCGG 7 cut(s) 233, 336, 425, 486, 586, 814, 1551
BslFI GGGAC 5 cut(s) 795, 1172, 1500, 1722, 1815
BsmAI GTCTC 3 cut(s) 411, 1485, 1934
BsmFI GGGAC 5 cut(s) 795, 1172, 1500, 1722, 1815
BsmI GAATGC 3 cut(s) 595, 1093, 2947
BsnI GGCC 6 cut(s) 140, 495, 1136, 1269, 2049, 2786
Bso31I GGTCTC 2 cut(s) 411, 1934
BsoBI CYCGRG 2 cut(s) 485, 721
Bsp1286I GDGCHC 9 cut(s) 117, 728, 805, 844, 1385, 1561, 1747, 2220, 2544
Bsp1407I TGTACA 1 cut(s) 2869
Bsp1720I GCTNAGC 1 cut(s) 2775
BspANI GGCC 6 cut(s) 140, 495, 1136, 1269, 2049, 2786
BspCNI CTCAG 6 cut(s) 285, 646, 799, 2687, 2767, 2788
BspFNI CGCG 1 cut(s) 277
BspHI TCATGA 1 cut(s) 1642
BspMAI CTGCAG 3 cut(s) 919, 1596, 2230
BspMI ACCTGC 1 cut(s) 3055
BspOI GCTAGC 2 cut(s) 585, 1196
BspPI GGATC 6 cut(s) 18, 224, 776, 1038, 2302, 2546
BspQI GCTCTTC 2 cut(s) 832, 1566
BspT107I GGYRCC 3 cut(s) 332, 564, 2807
BspTI CTTAAG 1 cut(s) 32
BspTNI GGTCTC 2 cut(s) 411, 1934
BsrDI GCAATG 2 cut(s) 1446, 2844
BsrGI TGTACA 1 cut(s) 2869
BssECI CCNNGG 7 cut(s) 275, 300, 485, 496, 720, 812, 1270
BssNAI GTATAC 2 cut(s) 2438, 2958
BssNI GRCGYC 2 cut(s) 189, 333
BssSI CACGAG 1 cut(s) 2982
BssT1I CCWWGG 2 cut(s) 300, 496
Bst1107I GTATAC 2 cut(s) 2438, 2958
Bst2BI CACGAG 1 cut(s) 2982
Bst2UI CCWGG 2 cut(s) 1860, 2783
Bst4CI ACNGT 9 cut(s) 53, 151, 762, 1307, 1737, 1880, 2222, 2496, 2924
Bst6I CTCTTC 5 cut(s) 506, 832, 969, 1566, 3008
BstACI GRCGYC 2 cut(s) 189, 333
BstAFI CTTAAG 1 cut(s) 32
BstAPI GCANNNNNTGC 3 cut(s) 1508, 1631, 2644
BstAUI TGTACA 1 cut(s) 2869
BstC8I GCNNGC 6 cut(s) 208, 583, 1194, 1632, 2592, 2720
BstDEI CTNAG 9 cut(s) 221, 293, 633, 786, 2279, 2674, 2775, 2796, 3063
BstDSI CCRYGG 1 cut(s) 275
BstENI CCTNNNNNAGG 1 cut(s) 1074
BstFNI CGCG 1 cut(s) 277
BstH2I RGCGCY 3 cut(s) 336, 2674, 2782
BstHHI GCGC 5 cut(s) 322, 335, 672, 2673, 2781
BstMAI GTCTC 3 cut(s) 411, 1485, 1934
BstNI CCWGG 2 cut(s) 1860, 2783
BstNSI RCATGY 4 cut(s) 1672, 2270, 2594, 2645
BstSCI CCNGG 5 cut(s) 484, 485, 812, 1858, 2781
BstSFI CTRYAG 4 cut(s) 915, 1592, 2226, 2319
BstUI CGCG 1 cut(s) 277
BstX2I RGATCY 2 cut(s) 781, 919
BstXI CCANNNNNNTGG 2 cut(s) 713, 2261
BstYI RGATCY 2 cut(s) 781, 919
BstZ17I GTATAC 2 cut(s) 2438, 2958
BsuRI GGCC 6 cut(s) 140, 495, 1136, 1269, 2049, 2786
BtgI CCRYGG 1 cut(s) 275
BtsI GCAGTG 3 cut(s) 1150, 1545, 2542
BveI ACCTGC 1 cut(s) 3055
Cac8I GCNNGC 6 cut(s) 208, 583, 1194, 1632, 2592, 2720
CaiI CAGNNNCTG 2 cut(s) 1508, 1511
CciI TCATGA 1 cut(s) 1642
CfoI GCGC 5 cut(s) 322, 335, 672, 2673, 2781
Cfr42I CCGCGG 1 cut(s) 278
Cfr9I CCCGGG 1 cut(s) 485
CseI GACGC 1 cut(s) 197
CsiI ACCWGGT 1 cut(s) 1858
Csp6I GTAC 2 cut(s) 2808, 2870
CspCI CAANNNNNGTGG 2 cut(s) 1126, 1161
CviQI GTAC 2 cut(s) 2808, 2870
DdeI CTNAG 9 cut(s) 221, 293, 633, 786, 2279, 2674, 2775, 2796, 3063
DinI GGCGCC 1 cut(s) 334
DrdI GACNNNNNNGTC 1 cut(s) 1863
DseDI GACNNNNNNGTC 1 cut(s) 1863
EaeI YGGCCR 2 cut(s) 1134, 1267
Eam1104I CTCTTC 5 cut(s) 506, 832, 969, 1566, 3008
EarI CTCTTC 5 cut(s) 506, 832, 969, 1566, 3008
EciI GGCGGA 1 cut(s) 477
Ecl136II GAGCTC 2 cut(s) 115, 842
Eco130I CCWWGG 2 cut(s) 300, 496
Eco24I GRGCYC 5 cut(s) 117, 728, 805, 844, 1385
Eco31I GGTCTC 2 cut(s) 411, 1934
Eco47I GGWCC 9 cut(s) 297, 1159, 1244, 1487, 1579, 1856, 2071, 2338, 2848
Eco47III AGCGCT 1 cut(s) 2672
Eco53kI GAGCTC 2 cut(s) 115, 842
Eco57I CTGAAG 5 cut(s) 515, 600, 1547, 2463, 2816
Eco88I CYCGRG 2 cut(s) 485, 721
EcoICRI GAGCTC 2 cut(s) 115, 842
EcoNI CCTNNNNNAGG 1 cut(s) 1074
EcoO109I RGGNCCY 2 cut(s) 1244, 2785
EcoRI GAATTC 1 cut(s) 1312
EcoRII CCWGG 2 cut(s) 1858, 2781
EcoT14I CCWWGG 2 cut(s) 300, 496
EcoT22I ATGCAT 3 cut(s) 595, 642, 972
EcoT38I GRGCYC 5 cut(s) 117, 728, 805, 844, 1385
EgeI GGCGCC 1 cut(s) 334
EheI GGCGCC 1 cut(s) 334
ErhI CCWWGG 2 cut(s) 300, 496
FalI AAGNNNNNCTT 6 cut(s) 393, 425, 553, 585, 829, 861
FaqI GGGAC 5 cut(s) 795, 1172, 1500, 1722, 1815
FauI CCCGC 2 cut(s) 318, 374
FauNDI CATATG 1 cut(s) 2509
FbaI TGATCA 2 cut(s) 2395, 2479
FblI GTMKAC 5 cut(s) 505, 1109, 1302, 2437, 2957
FriOI GRGCYC 5 cut(s) 117, 728, 805, 844, 1385
FspBI CTAG 6 cut(s) 582, 749, 798, 807, 1193, 1994
FspI TGCGCA 1 cut(s) 321
GlaI GCGC 5 cut(s) 321, 334, 671, 2672, 2780
GsaI CCCAGC 2 cut(s) 1259, 1344
GsuI CTGGAG 3 cut(s) 1479, 2669, 3027
HaeII RGCGCY 3 cut(s) 336, 2674, 2782
HaeIII GGCC 6 cut(s) 140, 495, 1136, 1269, 2049, 2786
HapII CCGG 7 cut(s) 233, 336, 425, 486, 586, 814, 1551
HgaI GACGC 1 cut(s) 197
HhaI GCGC 5 cut(s) 322, 335, 672, 2673, 2781
Hin1I GRCGYC 2 cut(s) 189, 333
Hin6I GCGC 5 cut(s) 320, 333, 670, 2671, 2779
HinP1I GCGC 5 cut(s) 320, 333, 670, 2671, 2779
HincII GTYRAC 2 cut(s) 196, 2499
HindII GTYRAC 2 cut(s) 196, 2499
HindIII AAGCTT 1 cut(s) 1698
HpaI GTTAAC 1 cut(s) 196
HpaII CCGG 7 cut(s) 233, 336, 425, 486, 586, 814, 1551
HphI GGTGA 3 cut(s) 410, 1016, 1356
Hpy166II GTNNAC 9 cut(s) 196, 506, 758, 1110, 1303, 2438, 2499, 2958, 3044
Hpy8I GTNNAC 9 cut(s) 196, 506, 758, 1110, 1303, 2438, 2499, 2958, 3044
Hpy99I CGWCG 1 cut(s) 176
HpyCH4III ACNGT 9 cut(s) 53, 151, 762, 1307, 1737, 1880, 2222, 2496, 2924
HpyF3I CTNAG 9 cut(s) 221, 293, 633, 786, 2279, 2674, 2775, 2796, 3063
Hsp92I GRCGYC 2 cut(s) 189, 333
HspAI GCGC 5 cut(s) 320, 333, 670, 2671, 2779
KasI GGCGCC 1 cut(s) 332
KpnI GGTACC 1 cut(s) 2811
Ksp22I TGATCA 2 cut(s) 2395, 2479
KspAI GTTAAC 1 cut(s) 196
KspI CCGCGG 1 cut(s) 278
LguI GCTCTTC 2 cut(s) 832, 1566
MabI ACCWGGT 1 cut(s) 1858
MaeI CTAG 6 cut(s) 582, 749, 798, 807, 1193, 1994
MaeIII GTNAC 6 cut(s) 1362, 1880, 2356, 2372, 2488, 2791
MflI RGATCY 2 cut(s) 781, 919
MhlI GDGCHC 9 cut(s) 117, 728, 805, 844, 1385, 1561, 1747, 2220, 2544
MlsI TGGCCA 1 cut(s) 1136
MluNI TGGCCA 1 cut(s) 1136
Mly113I GGCGCC 1 cut(s) 333
MlyI GAGTC 4 cut(s) 920, 1448, 2341, 3025
MmeI TCCRAC 3 cut(s) 334, 808, 1834
Mox20I TGGCCA 1 cut(s) 1136
Mph1103I ATGCAT 3 cut(s) 595, 642, 972
MroXI GAANNNNTTC 2 cut(s) 2210, 2829
MscI TGGCCA 1 cut(s) 1136
MseI TTAA 9 cut(s) 33, 195, 1044, 1203, 1263, 1601, 2756, 2919, 2928
MslI CAYNNNNRTG 5 cut(s) 735, 1673, 2508, 2547, 2940
Msp20I TGGCCA 1 cut(s) 1136
MspA1I CMGCKG 4 cut(s) 277, 292, 325, 1508
MspCI CTTAAG 1 cut(s) 32
MspI CCGG 7 cut(s) 233, 336, 425, 486, 586, 814, 1551
MspR9I CCNGG 5 cut(s) 486, 487, 814, 1860, 2783
Mva1269I GAATGC 3 cut(s) 595, 1093, 2947
MvaI CCWGG 2 cut(s) 1860, 2783
MvnI CGCG 1 cut(s) 277
NarI GGCGCC 1 cut(s) 333
NciI CCSGG 3 cut(s) 486, 487, 814
NdeI CATATG 1 cut(s) 2509
NheI GCTAGC 2 cut(s) 581, 1192
NmeAIII GCCGAG 2 cut(s) 547, 1295
NmuCI GTSAC 5 cut(s) 1362, 1880, 2356, 2372, 2488
NsbI TGCGCA 1 cut(s) 321
NsiI ATGCAT 3 cut(s) 595, 642, 972
NspI RCATGY 4 cut(s) 1672, 2270, 2594, 2645
PaeI GCATGC 1 cut(s) 2594
PagI TCATGA 1 cut(s) 1642
PaqCI CACCTGC 1 cut(s) 3055
PciSI GCTCTTC 2 cut(s) 832, 1566
PctI GAATGC 3 cut(s) 595, 1093, 2947
PdmI GAANNNNTTC 2 cut(s) 2210, 2829
PfeI GAWTC 8 cut(s) 73, 526, 817, 979, 997, 1073, 1774, 2200
PflFI GACNNNGTC 1 cut(s) 2494
PflMI CCANNNNNTGG 2 cut(s) 827, 1802
PleI GAGTC 4 cut(s) 919, 1448, 2341, 3025
PluTI GGCGCC 1 cut(s) 336
PpsI GAGTC 4 cut(s) 919, 1448, 2341, 3025
PpuMI RGGWCCY 1 cut(s) 1244
Psp124BI GAGCTC 2 cut(s) 117, 844
Psp5II RGGWCCY 1 cut(s) 1244
Psp6I CCWGG 2 cut(s) 1858, 2781
PspFI CCCAGC 2 cut(s) 1255, 1340
PspGI CCWGG 2 cut(s) 1858, 2781
PspPPI RGGWCCY 1 cut(s) 1244
PstI CTGCAG 3 cut(s) 919, 1596, 2230
PstNI CAGNNNCTG 2 cut(s) 1508, 1511
PsuI RGATCY 2 cut(s) 781, 919
PsyI GACNNNGTC 1 cut(s) 2494
PvuII CAGCTG 1 cut(s) 1508
RsaI GTAC 2 cut(s) 2809, 2871
RsaNI GTAC 2 cut(s) 2808, 2870
RseI CAYNNNNRTG 5 cut(s) 735, 1673, 2508, 2547, 2940
SacI GAGCTC 2 cut(s) 117, 844
SacII CCGCGG 1 cut(s) 278
SapI GCTCTTC 2 cut(s) 832, 1566
SaqAI TTAA 9 cut(s) 33, 195, 1044, 1203, 1263, 1601, 2756, 2919, 2928
SchI GAGTC 4 cut(s) 920, 1448, 2341, 3025
ScrFI CCNGG 5 cut(s) 486, 487, 814, 1860, 2783
SduI GDGCHC 9 cut(s) 117, 728, 805, 844, 1385, 1561, 1747, 2220, 2544
SexAI ACCWGGT 1 cut(s) 1858
SfcI CTRYAG 4 cut(s) 915, 1592, 2226, 2319
SfoI GGCGCC 1 cut(s) 334
Sfr303I CCGCGG 1 cut(s) 278
SgrBI CCGCGG 1 cut(s) 278
SinI GGWCC 9 cut(s) 297, 1159, 1244, 1487, 1579, 1856, 2071, 2338, 2848
SmaI CCCGGG 1 cut(s) 487
SmiMI CAYNNNNRTG 5 cut(s) 735, 1673, 2508, 2547, 2940
SmlI CTYRAG 3 cut(s) 32, 559, 1237
SmoI CTYRAG 3 cut(s) 32, 559, 1237
SphI GCATGC 1 cut(s) 2594
SspDI GGCGCC 1 cut(s) 332
SspMI CTAG 6 cut(s) 582, 749, 798, 807, 1193, 1994
SstI GAGCTC 2 cut(s) 117, 844
StyD4I CCNGG 5 cut(s) 484, 485, 812, 1858, 2781
StyI CCWWGG 2 cut(s) 300, 496
TaaI ACNGT 9 cut(s) 53, 151, 762, 1307, 1737, 1880, 2222, 2496, 2924
TaqI TCGA 3 cut(s) 1968, 2214, 2302
TatI WGTACW 1 cut(s) 2869
TauI GCSGC 6 cut(s) 280, 289, 292, 333, 440, 443
TfiI GAWTC 8 cut(s) 73, 526, 817, 979, 997, 1073, 1774, 2200
Tru1I TTAA 9 cut(s) 33, 195, 1044, 1203, 1263, 1601, 2756, 2919, 2928
Tru9I TTAA 9 cut(s) 33, 195, 1044, 1203, 1263, 1601, 2756, 2919, 2928
TseFI GTSAC 5 cut(s) 1362, 1880, 2356, 2372, 2488
Tsp45I GTSAC 5 cut(s) 1362, 1880, 2356, 2372, 2488
TspMI CCCGGG 1 cut(s) 485
Tth111I GACNNNGTC 1 cut(s) 2494
Van91I CCANNNNNTGG 2 cut(s) 827, 1802
Vha464I CTTAAG 1 cut(s) 32
VpaK11BI GGWCC 9 cut(s) 297, 1159, 1244, 1487, 1579, 1856, 2071, 2338, 2848
XagI CCTNNNNNAGG 1 cut(s) 1074
XapI RAATTY 8 cut(s) 877, 1051, 1312, 1492, 1963, 2040, 2532, 2726
XceI RCATGY 4 cut(s) 1672, 2270, 2594, 2645
XmaI CCCGGG 1 cut(s) 485
XmiI GTMKAC 5 cut(s) 505, 1109, 1302, 2437, 2957
XmnI GAANNNNTTC 2 cut(s) 2210, 2829
XspI CTAG 6 cut(s) 582, 749, 798, 807, 1193, 1994
Zsp2I ATGCAT 3 cut(s) 595, 642, 972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.